Hirofumi Noguchi

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi BETA2/NeuroD protein transduction requires cell surface heparan sulfate proteoglycans
    Hirofumi Noguchi
    Diabetes Research Institute Japan, Toyoake, Aichi 470 1192, Japan
    Hum Gene Ther 18:10-7. 2007
  2. ncbi Ductal injection of preservation solution increases islet yields in islet isolation and improves islet graft function
    Hirofumi Noguchi
    Transplantation Unit, Kyoto University Hospital, Kyoto 606 8507, Japan
    Cell Transplant 17:69-81. 2008
  3. ncbi Cell permeable peptide of JNK inhibitor prevents islet apoptosis immediately after isolation and improves islet graft function
    Hirofumi Noguchi
    Department of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Am J Transplant 5:1848-55. 2005
  4. ncbi Immunosuppression for islet transplantation
    Hirofumi Noguchi
    Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Acta Med Okayama 60:71-6. 2006
  5. ncbi Protein transduction technology: a novel therapeutic perspective
    Hirofumi Noguchi
    Diabetes Research Institute Kyoto, Kyoto 606 8507, Japan
    Acta Med Okayama 60:1-11. 2006
  6. ncbi Modified two-layer preservation method (M-Kyoto/PFC) improves islet yields in islet isolation
    H Noguchi
    Diabetes Research Institute Kyoto, Kyoto University Graduate School of Medicine, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Am J Transplant 6:496-504. 2006
  7. ncbi PDX-1 protein is internalized by lipid raft-dependent macropinocytosis
    Hirofumi Noguchi
    Department of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Sakyo ku, Japan
    Cell Transplant 14:637-45. 2005
  8. ncbi BETA2/NeuroD protein can be transduced into cells due to an arginine- and lysine-rich sequence
    Hirofumi Noguchi
    Dept of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Sakyo ku, Kyoto 606 8507, Japan
    Diabetes 54:2859-66. 2005
  9. ncbi Mechanism of PDX-1 protein transduction
    Hirofumi Noguchi
    Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Biochem Biophys Res Commun 332:68-74. 2005
  10. ncbi Comparison of trypsin inhibitors in preservation solution for islet isolation
    Hirofumi Noguchi
    Transplantation Unit, Kyoto University Hospital, Kyoto 606 8507, Japan
    Cell Transplant 18:541-7. 2009

Collaborators

Detail Information

Publications68

  1. ncbi BETA2/NeuroD protein transduction requires cell surface heparan sulfate proteoglycans
    Hirofumi Noguchi
    Diabetes Research Institute Japan, Toyoake, Aichi 470 1192, Japan
    Hum Gene Ther 18:10-7. 2007
    ....
  2. ncbi Ductal injection of preservation solution increases islet yields in islet isolation and improves islet graft function
    Hirofumi Noguchi
    Transplantation Unit, Kyoto University Hospital, Kyoto 606 8507, Japan
    Cell Transplant 17:69-81. 2008
    ..Based on these data, this technique is now used for clinical islet transplantation from non-heart-beating donor pancreata or living donor pancreas...
  3. ncbi Cell permeable peptide of JNK inhibitor prevents islet apoptosis immediately after isolation and improves islet graft function
    Hirofumi Noguchi
    Department of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Am J Transplant 5:1848-55. 2005
    ..These findings suggest that peptide drugs could be useful for the prevention of the impairment of islet cells and lead to improvement in the outcomes for pancreatic islet transplantation...
  4. ncbi Immunosuppression for islet transplantation
    Hirofumi Noguchi
    Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700 8558, Japan
    Acta Med Okayama 60:71-6. 2006
    ..In this review, we show some of the most recent contributions to the advancement of knowledge in this field...
  5. ncbi Protein transduction technology: a novel therapeutic perspective
    Hirofumi Noguchi
    Diabetes Research Institute Kyoto, Kyoto 606 8507, Japan
    Acta Med Okayama 60:1-11. 2006
    ..The protein transduction systems have opened up several possibilities, not only for the development of new peptide/protein drugs but also for consideration of their physiological and developmental implications...
  6. ncbi Modified two-layer preservation method (M-Kyoto/PFC) improves islet yields in islet isolation
    H Noguchi
    Diabetes Research Institute Kyoto, Kyoto University Graduate School of Medicine, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Am J Transplant 6:496-504. 2006
    ..In conclusion, the advantages of M-Kyoto solution are trypsin inhibition and less collagenase inhibition. Based on these data, we now use M-Kyoto solution for clinical islet transplantation from nonheart-beating donor pancreata...
  7. ncbi PDX-1 protein is internalized by lipid raft-dependent macropinocytosis
    Hirofumi Noguchi
    Department of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Sakyo ku, Japan
    Cell Transplant 14:637-45. 2005
    ..Taken together, these observations provide the mechanism of PDX-1 protein transduction and suggest that the protein transduction system could work for experimental and therapeutic strategies...
  8. ncbi BETA2/NeuroD protein can be transduced into cells due to an arginine- and lysine-rich sequence
    Hirofumi Noguchi
    Dept of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Sakyo ku, Kyoto 606 8507, Japan
    Diabetes 54:2859-66. 2005
    ..These data suggest that BETA2/NeuroD protein transduction could be a safe and valuable strategy for enhancing insulin gene transcription without requiring gene transfer technology...
  9. ncbi Mechanism of PDX-1 protein transduction
    Hirofumi Noguchi
    Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Biochem Biophys Res Commun 332:68-74. 2005
    ....
  10. ncbi Comparison of trypsin inhibitors in preservation solution for islet isolation
    Hirofumi Noguchi
    Transplantation Unit, Kyoto University Hospital, Kyoto 606 8507, Japan
    Cell Transplant 18:541-7. 2009
    ..These data suggest that ET-Kyoto with ulinastatin was the better combination for pancreas preservation than ET-Kyoto with Pefabloc. Based on these data, we now use ET-Kyoto solution with ulinastatin for clinical islet transplantation...
  11. ncbi Reestablishment of microenvironment is necessary to maintain in vitro and in vivo human islet function
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 17:111-9. 2008
    ..These studies indicate that the reestablishment of the cell-matrix interactions by a synthetic matrix in the immediate postisolation period is a useful tool to maintain islet functions in vitro and in vivo...
  12. doi Establishment of an immortalized porcine liver cell line JSNK-1 with retroviral transduction of SV40T
    Javed M Shahid
    Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Cell Transplant 19:849-56. 2010
    ..In conclusion, JSNK-1 cells would be valuable in the study of liver stellate cell pathophysiology and contribute to the optimization of hepatic differentiation of iPS cells...
  13. ncbi Living donor islet transplantation, the alternative approach to overcome the obstacles limiting transplant
    Yasuhiro Iwanaga
    Department of Transplantation and Immunology, Kyoto University Graduate School of Medicine, 54 Kawara cho Shogoin, Sakyo ku, Kyoto, Japan 606 8507
    Ann N Y Acad Sci 1079:335-9. 2006
    ..These are important factors in overcoming the obstacles limiting islet transplantation. We believe that the living donor islet transplantation may become an additional option in treating insulin-dependent diabetes...
  14. ncbi Amelioration of diabetes in mice after single-donor islet transplantation using the controlled release of gelatinized FGF-2
    Jorge David Rivas-Carrillo
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 15:939-44. 2006
    ..In contrast, the transplantation of islets without FGF-2 supplementation showed poor revascularization and failed to control the blood glucose levels in the diabetic mice...
  15. ncbi Secretory unit of islet in transplantation (SUIT) and engrafted islet rate (EIR) indexes are useful for evaluating single islet transplantation
    Hirofumi Noguchi
    Transplantation Unit, Kyoto University Hospital, Kyoto 606 8507, Japan
    Cell Transplant 17:121-8. 2008
    ..The SUIT and EIR indexes are thus considered to be useful values for evaluating islet transplantation, especially for single islet transplantation...
  16. ncbi Neovascularization induced around an artificial device implanted in the abdomen by the use of gelatinized fibroblast growth factor 2
    Takeshi Yuasa
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 18:683-8. 2009
    ..There were only minor instances of fibrosis and inflammation reaction around the BAP, thus indicating the BAP that we are currently developing to have an excellent histocompatibility...
  17. ncbi Maintenance of neovascularization at the implantation site of an artificial device by bFGF and endothelial cell transplant
    Atsushi Miki
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Cell Transplant 15:893-901. 2006
    ..We believe that the present study will contribute to BAP development for the treatment of diabetes...
  18. ncbi Laparoscopy-assisted creation of a liver failure model in pigs
    Takeshi Yuasa
    Department of Gastroenterological Surgery, Transplant, and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Cell Transplant 17:187-93. 2008
    ..We believe that our model is the first report on creation of a reliable model for liver failure in pigs to assess the efficacy of liver-targeted cell therapies...
  19. ncbi Effective islet isolation method with extremely high islet yields from adult pigs
    Yukihide Yonekawa
    Department of Transplantation and Immunology, Graduate School of Medicine, Kyoto University, Sakyo ku, Japan
    Cell Transplant 14:757-62. 2005
    ..It should produce useful information for human islet isolation and transplantation, and might be applied to single donor clinical xenogeneic transplantation...
  20. ncbi Comparison of M-Kyoto solution and histidine-tryptophan-ketoglutarate solution with a trypsin inhibitor for pancreas preservation in islet transplantation
    Hirofumi Noguchi
    Transplantation Unit, Kyoto University Hospital, Shogoin, Kyoto, Japan
    Transplantation 84:655-8. 2007
    ..In conclusion, M-Kyoto/PFC solution is better for pancreas preservation before islet isolation than M-HTK/PFC solution...
  21. pmc Isolation and propagation of a human CD133(-) colon tumor-derived cell line with tumorigenic and angiogenic properties
    Nalu Navarro-Alvarez
    Department of Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Cell Transplant 19:865-77. 2010
    ..In addition, NANK secreted high amounts of important angiogenic cytokines. These results provide a novel and extensive model in human CSC for studying the generation and maintenance of phenotypic heterogeneity in CRC...
  22. pmc Treatment of acute liver failure in mice by hepatocyte xenotransplantation
    Tsuyoshi Yamamoto
    Department of Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Cell Transplant 19:799-806. 2010
    ..Hepatocytes culture in a truly 3D network allows in vivo maintaining of differentiated functions, and once transplanted between widely divergent species can function to correct acute liver failure in mice and prolong their survival...
  23. doi Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice
    Hiroyuki Nakahara
    Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Transplantation 88:346-53. 2009
    ..The hMSCs may provide a potential therapy to restore damaged tissues or organs of mesenchymal origin; however, a drawback is their limited life span in vitro...
  24. ncbi Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts
    Atsushi Miki
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Cell Transplant 15:325-34. 2006
    ..A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system...
  25. doi A novel gene expression system for detecting viable bladder cancer cells
    Hideo Ueki
    Center for Gene and Cell Therapy, Okayama University, Okayama 700 8558, Japan
    Int J Oncol 41:135-40. 2012
    ..The current system can be applied for in vitro detection of bladder cancer cells in urine and other types of cancer cells disseminated in vivo...
  26. doi Attenuation of murine graft-versus-host disease by a tea polyphenol
    Jun Kanamune
    Transplant Unit, Kyoto University Hospital, Kyoto, Japan
    Cell Transplant 21:909-18. 2012
    ..These results strongly suggest that EGCG attenuates GVHD by both blocking specific cell surface molecules and affecting the donor T-cell proliferation pathways...
  27. ncbi A new mouse model for intraportal islet transplantation with limited hepatic lobe as a graft site
    Yukihide Yonekawa
    Department of Transplantation and Immunology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Transplantation 82:712-5. 2006
    ..This model can be applied to investigate both the specific graft-recipient interaction in the liver and the islet graft contribution to the control of diabetes...
  28. ncbi Successful islet transplantation from nonheartbeating donor pancreata using modified Ricordi islet isolation method
    Shinichi Matsumoto
    Kyoto University Hospital Transplantation Unit, Kyoto, Japan
    Transplantation 82:460-5. 2006
    ..The goal of this study was to improve the efficacy of islet transplantation using nonheartbeating donors (NHBDs)...
  29. ncbi Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with Tamoxifen-mediated self-recombination
    Toshinori Totsugawa
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    J Hepatol 47:74-82. 2007
    ..Hepatocyte transplantation and bioartificial liver treatment are attractive alternatives to liver transplantation. The availability of well-characterized human hepatocyte lines facilitates such cell therapies...
  30. doi Preclinical biodistribution and safety study of reduced expression in immortalized cells/Dickkopf-3-encoding adenoviral vector for prostate cancer gene therapy
    Morito Sugimoto
    Department of Urology, Okayama University, Okayama 700 8558, Japan
    Oncol Rep 28:1645-52. 2012
    ..Based on these preclinical experiments, adenovirus-mediated in situ REIC/Dkk-3 gene therapy is considered to be safe for use as a treatment for human prostate cancer...
  31. ncbi Photo-acceleration of protein release from endosome in the protein transduction system
    Masayuki Matsushita
    Department of Physiology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    FEBS Lett 572:221-6. 2004
    ..Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system...
  32. ncbi Pancreatic islet transplantation for treating diabetes
    Shinichi Matsumoto
    Transplantation Unit, Kyoto University Hospital, Diabetes Research Institute Kyoto, Shogoin, Kyoto 606 8507, Japan
    Expert Opin Biol Ther 6:23-37. 2006
    ....
  33. ncbi Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes
    Alejandro Soto-Gutierrez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Nat Biotechnol 24:1412-9. 2006
    ..After functioning in the BAL, ES cell-derived hepatocytes developed characteristics nearly identical to those of primary hepatocytes...
  34. ncbi PDX-1 protein containing its own antennapedia-like protein transduction domain can transduce pancreatic duct and islet cells
    Hirofumi Noguchi
    Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 52:1732-7. 2003
    ....
  35. ncbi A single transplantation of the islets can produce glycemic stability and reduction of basal insulin requirement
    Mariko Sassa
    Department of Diabetes and Clinical Nutrition, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Diabetes Res Clin Pract 73:235-40. 2006
    ....
  36. ncbi Protein transduction technology offers a novel therapeutic approach for diabetes
    Hirofumi Noguchi
    Diabetes Research Institute Kyoto, Kyoto, Japan
    J Hepatobiliary Pancreat Surg 13:306-13. 2006
    ..Protein transduction technology has opened up several possibilities for the development of new peptide/protein drugs for the treatment of diabetes...
  37. ncbi Free jejunal graft for hypopharyngeal and esophageal reconstruction
    Yasuhiro Shirakawa
    Department of Gastroenterological Surgery, Transplant, and Surgical Oncology, Graduate School of Medicine and Dentistry, Okayama University, 2 5 1 Shikatacho, Okayama 700 8558, Japan
    Langenbecks Arch Surg 389:387-90. 2004
    ..This study assessed the techniques of the free jejunal graft for the reconstruction of hypopharynx or cervical esophagus and discussed the main aspects related to those procedures...
  38. doi Transplantation of osteogenically differentiated mouse iPS cells for bone repair
    Takahiro Hayashi
    Department of Orthopeadic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    Cell Transplant 21:591-600. 2012
    ..It is believed that irradiation before transplantation is an effective way to inhibit teratoma formation...
  39. ncbi Procurement of the human pancreas for pancreatic islet transplantation from marginal cadaver donors
    Hideo Nagata
    Department of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Transplantation 82:327-31. 2006
    ..The specific aim of this study was to develop an effective technique for the procurement of pancreas for PIT from nonheart-beating-donor (NHBDs)...
  40. ncbi A human beta-cell line for transplantation therapy to control type 1 diabetes
    Michiki Narushima
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Nat Biotechnol 23:1274-82. 2005
    ..The establishment of this cell line is one step toward a potential cure of diabetes by transplantation...
  41. ncbi Tea polyphenol inhibits allostimulation in mixed lymphocyte culture
    Jong yoon Kim
    Department of Simulation Medical Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell Transplant 16:75-83. 2007
    ..Other possible mechanisms behind the immunosuppressive effect and a potential use of EGCG treatment of donor tissues in transplantation medicine are discussed...
  42. ncbi Controlled expansion of human endothelial cell populations by Cre-loxP-based reversible immortalization
    Hirofumi Noguchi
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Hum Gene Ther 13:321-34. 2002
    ..This study extends the utility of the reversible immortalization procedure and provides a means to expand primary human ECs of various sources for basic studies and possible cell and gene therapies...
  43. ncbi [Islet transplantation]
    Hirofumi Noguchi
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    Nihon Rinsho 69:2209-13. 2011
    ..Recent progress in the search for new sources of beta cells has opened up several possibilities for the development of new treatments for diabetes...
  44. ncbi Insulin independence after living-donor distal pancreatectomy and islet allotransplantation
    Shinichi Matsumoto
    Kyoto University Hospital Transplantation Unit, Kyoto Japan
    Lancet 365:1642-4. 2005
    ..The donor had no complications and both women showed healthy glucose tolerance. Transplantation of living-donor islets from the distal pancreas can be sufficient to reverse brittle diabetes...
  45. doi Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1
    Kazuhiko Ochiai
    Innovation Center Okayama for Nanobio Targeted Therapy, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan
    Biochem Biophys Res Commun 412:391-5. 2011
    ..The link between REIC/Dkk-3 and Tctex-1 may be of significance for understanding the molecular functions of the proteins in ER stress signaling and intracellular dynein motor dynamics, respectively...
  46. pmc Diagnosis and evaluation of nonalcoholic fatty liver disease
    Mikako Obika
    Department of General Internal Medicine, Okayama University Hospital, 2 5 1 Shikata cho, Okayama 700 8558, Japan
    Exp Diabetes Res 2012:145754. 2012
    ..Therefore, new investigations of the pathogenesis of NAFLD are necessary to develop useful biomarkers that could provide a reliable noninvasive alternative to liver biopsy...
  47. ncbi Three cases of adenocarcinoma arising in extremely long-segment Barrett's esophagus
    Toshiya Fujiwara
    Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Graduate School of Medicine and Dentistry, Okayama University, 2 5 1 Shikata cho, Okayama, Japan
    Dig Dis Sci 51:533-8. 2006
  48. ncbi Primary malignant melanoma of the esophagus: long-term survival following pre- and postoperative adjuvant hormone/chemotherapy
    Hirokazu Uetsuka
    Department of Gastroenterological Surgery, Transplant, and Surgical Oncology, Graduate School of Medicine and Dentistry, Okayama University, 2 5 1 Shikatacho, Okayama 700 8558, Japan
    Dig Dis Sci 49:1646-51. 2004
  49. ncbi Cell-permeable pentapeptide V5 inhibits apoptosis and enhances insulin secretion, allowing experimental single-donor islet transplantation in mice
    Jorge D Rivas-Carrillo
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Shikata cho, Okayama, Japan
    Diabetes 56:1259-67. 2007
    ..Following isolation and transplantation, islets are susceptible to apoptosis, which limits their function and probably long-term islet graft survival...
  50. ncbi Activation of c-Jun NH2-terminal kinase during islet isolation
    Hirofumi Noguchi
    Diabetes Research Institute Japan, Japan
    Endocr J 54:169-76. 2007
    ..These findings suggest that the control of JNK activation is important for pancreatic islet transplantation...
  51. ncbi Induction of pancreatic stem/progenitor cells into insulin-producing cells by adenoviral-mediated gene transfer technology
    Hirofumi Noguchi
    Diabetes Research Institute Japan, Toyoake, Aichi 470 1192, Japan
    Cell Transplant 15:929-38. 2006
    ..These data suggest that the overexpression of transcription factors, especially NeuroD, facilitates pancreatic stem/progenitor cell differentiation into insulin-producing cells...
  52. ncbi UW solution: a promising tool for cryopreservation of primarily isolated rat hepatocytes
    Jun Arikura
    Second Department of Surgery, Asahikawa Medical College, 2 1 1 1 Midorigaoka Higashi, Asahikawa 078 8510, Japan
    J Hepatobiliary Pancreat Surg 9:742-9. 2002
    ..Plating efficiency and ammonia clearance activity were improved in G1 hepatocytes compared to G2 and G3 cells. Lentiviral transfer of a LacZ gene was confirmed in the thawed hepatocytes after cryopreservation by an X-gal stain assay...
  53. ncbi Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers
    Takamasa Watanabe
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 75:1873-80. 2003
    ..Because the limited lifespan of human HSC is a major hurdle to their use, the authors report here the amplification of human HSC populations in vitro by retroviral transfer of human telomerase reverse transcriptase (hTERT)...
  54. ncbi A newly developed bioartificial pancreas successfully controls blood glucose in totally pancreatectomized diabetic pigs
    Hideaki Ikeda
    Department of Surgery, Graduate School of Medicine and Dentistry, Okayama University, Okayama, Japan
    Tissue Eng 12:1799-809. 2006
    ..These results indicate that our newly developed BAP may be a potential therapy for the treatment of diabetes in humans...
  55. ncbi A new cell-permeable peptide allows successful allogeneic islet transplantation in mice
    Hirofumi Noguchi
    Department of Physiology, Okayama University Graduate School of Medicine and Dentistry, 2 5 1 Shikata cho, Okayama, 700 8558 Japan
    Nat Med 10:305-9. 2004
    ..In addition, it did not affect insulin secretion, whereas FK506 caused a dose-dependent decrease in insulin secretion. Cell-permeable peptides can thus provide a new strategy for drug development and may eventually be useful clinically...
  56. ncbi Establishment of an immortalized human-liver endothelial cell line with SV40T and hTERT
    Toshihisa Matsumura
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 77:1357-65. 2004
    ..We also demonstrate excision of SV40T and hTERT with TAT-mediated Cre/loxP recombination and subsequent cell sorting...
  57. ncbi Analysis of donor- and isolation-related variables from non-heart-beating donors (NHBDs) using the Kyoto islet isolation method
    Xiaoling Liu
    General Surgery, First Clinical College of Harbin Medical University, Harbin, PR China
    Cell Transplant 17:649-56. 2008
    ..KIIM enables us to perform islet transplantation from NHBDs; however, the decision to use pancreata from older donors or those with chronic pancreatitis requires careful consideration...
  58. ncbi Recombinant sendai virus-mediated gene transfer to adipose tissue-derived stem cells (ASCs)
    Hiroshi Yukawa
    Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 461 0047, Japan
    Cell Transplant 17:43-50. 2008
    ..In conclusion, this is the first report describing the successful use of SeV-mediated gene transfer in ASCs, and the results indicate that SeV may thus provide advantages with respect to safety issues in gene therapy...
  59. ncbi Temporal decline in sirolimus elimination immediately after pancreatic islet transplantation
    Eriko Sato
    Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University
    Drug Metab Pharmacokinet 21:492-500. 2006
    ..A high trough concentration of sirolimus might increase the risk of hematological toxicy, and adjustment of the dosage for immunosuppressive treatment will be necessary in Japanese patients...
  60. ncbi Cell surface heparan sulfate proteoglycans mediate the internalization of PDX-1 protein
    Michiko Ueda
    Fujita Health University, Second Department of Surgery, Aichi 470 1192, Japan
    Cell Transplant 17:91-7. 2008
    ....
  61. ncbi Pancreatic islet cell transplantation: update and new developments
    Nicholas Onaca
    Transplant Services, Baylor Regional Transplant Institute, Baylor University Medical Center, Dallas, TX 75246, USA
    Nutr Clin Pract 22:485-93. 2007
    ....
  62. ncbi Role of PDX-1 and MafA as a potential therapeutic target for diabetes
    Hideaki Kaneto
    Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Osaka, Japan
    Diabetes Res Clin Pract 77:S127-37. 2007
    ..Taken together, PDX-1 and MafA play a crucial role in inducing surrogate beta-cells and could be a therapeutic target for diabetes...
  63. ncbi Prolonged survival of mice with acute liver failure with transplantation of monkey hepatocytes cultured with an antiapoptotic pentapeptide V5
    Kimiaki Tanaka
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 81:427-37. 2006
    ..We here report the usefulness of an antiapoptotic pentapeptide V5, composed of Val-Pro-Met-Leu-Lys, in the monkey hepatocyte cultures...
  64. ncbi Follow-up study of the first successful living donor islet transplantation
    Shinichi Matsumoto
    2nd Department of Surgery, Fujita Health University, Aichi, Japan
    Transplantation 82:1629-33. 2006
    ..However, we have very limited number of such donors in Japan; therefore, it is not allowed to perform islet transplantation with brain-dead donors. In order to perform islet transplantation in Japan, we need to seek new donor resources...
  65. ncbi Construction and transplantation of an engineered hepatic tissue using a polyaminourethane-coated nonwoven polytetrafluoroethylene fabric
    Alejandro Soto-Gutierrez
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
    Transplantation 83:129-37. 2007
    ..Here, we evaluate the construction and transplantation of an engineered hepatic tissue (EHT) using primary isolated hepatocytes cultured onto polyaminourethane (PAU)-coated, nonwoven polytetrafluoroethylene (PTFE) fabric...
  66. ncbi Lentiviral transfer of the LacZ gene into human endothelial cells and human bone marrow mesenchymal stem cells
    Toshinori Totsugawa
    Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Japan
    Cell Transplant 11:481-8. 2002
    ..These findings provide evidence that lentiviral vectors are efficient tools for gene transfer and expression in human endothelial cells and stem cells that could be useful for tissue engineering...
  67. ncbi Transduction of immortalized human hepatocytes with p21 to enhance differentiated phenotypes
    Takemi Kunieda
    Department of Medicine, Kawasaki Medical School, Kurashiki, Okayama, Japan
    Cell Transplant 11:421-8. 2002
    ..We here show that exogenous expression of p21 augments cellular differentiation in immortalized human NKNT-3 cells...
  68. ncbi Cryopreservation of mouse adipose tissue-derived stem/progenitor cells
    Koichi Oishi
    Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 466 8550, Japan
    Cell Transplant 17:35-41. 2008
    ..These data suggests that Cell Banker 2 is the most effective cryopreservation solution for ASCs and that cryopreserved as well as noncryopreserved ASCs could be applied for regenerative medicine...