Research Topics
Genomes and Genes
| Kazuhisa NakayamaSummaryAffiliation: Kyoto University Country: Japan Publications
| Collaborators
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Detail Information
Publications
Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulationChisa Shinotsuka
Institute of Biological Sciences and Gene Research Center, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba Science City, Ibaraki 305 8572, Japan
J Biol Chem 277:9468-73. 2002..These observations indicate that BFA-induced AP-1 dissociation from TGN membranes and tubulation of TGN membranes are not coupled events and suggest that a BFA target other than ARF-GEFs exists in the cell...- The structure and function of GGAs, the traffic controllers at the TGN sorting crossroadsKazuhisa Nakayama
Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8501, Japan
Cell Struct Funct 28:431-42. 2003..Together with or independent of the adaptor protein complex AP-1, GGAs regulate selective transport of cargo proteins, such as mannose 6-phosphate receptors, from the trans-Golgi network to endosomes mediated by clathrin-coated vesicles... - [Regulation of membrane traffic by ubiquitin]Kazuhisa Nakayama
Tanpakushitsu Kakusan Koso 51:1325-30. 2006 - Guanine nucleotide-exchange factors for arf GTPases: their diverse functions in membrane trafficHye Won Shin
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Biochem 136:761-7. 2004..Here we review the structures and functions of Arf-GEFs by focusing on the regulation of membrane traffic... - Membrane traffic: editorial overviewKazuhisa Nakayama
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Biochem 136:751-3. 2004..This Minireview series composed of eight articles highlights the recent progress in this rapidly expanding research field... - Differential effects of depletion of ARL1 and ARFRP1 on membrane trafficking between the trans-Golgi network and endosomesKirika Nishimoto-Morita
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Biol Chem 284:10583-92. 2009..Furthermore, we have obtained evidence suggesting that a SNARE complex containing Vti1a, syntaxin 6, and syntaxin 16 is involved in Shiga toxin transport downstream of ARL1... - Recruitment of Tom1L1/Srcasm to endosomes and the midbody by Tsg101Yuko Yanagida-Ishizaki
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
Cell Struct Funct 33:91-100. 2008..Taken into account the topological equivalency among the events of the MVB formation, viral egress from the cell, and cytokinesis, the data obtained here suggest that Tom1L1 is implicated in these three distinct cellular processes... - Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomesRay Ishizaki
Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
Mol Biol Cell 19:2650-60. 2008..Taken together with previous reports, these observations indicate that BIG2 and BIG1 play redundant roles in trafficking between the TGN and endosomes that involves the AP-1 complex... - Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4Hideaki Yamamoto
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
Mol Biol Cell 21:2746-55. 2010..Depletion of Rab14 or RUFY1, as well as Rab4, inhibits efficient recycling of endocytosed transferrin, suggesting that Rab14 and Rab4 regulate endosomal functions through cooperative interactions with their dual effector, RUFY1... - Distinct roles of Rab11 and Arf6 in the regulation of Rab11-FIP3/arfophilin-1 localization in mitotic cellsSenye Takahashi
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Japan
Genes Cells 16:938-50. 2011.... - Involvement of CD44 in mast cell proliferation during terminal differentiationHirotsugu Takano
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
Lab Invest 89:446-55. 2009..These results suggest that CD44 plays a crucial role in the regulation of the cutaneous mast cell number... - GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasisKouhei Takashima
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
Cell Struct Funct 36:223-35. 2011..On the basis of these results, we propose other mechanisms how the GBF1-Arf-COPI-ArfGAP transport machinery is implicated in the regulation of lipid homeostasis... - Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane traffickingHye Won Shin
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
J Cell Sci 118:4039-48. 2005.... - AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factorsRay Ishizaki
Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
Genes Cells 11:949-59. 2006.... - Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not ArfsZhiqiu Man
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
J Biol Chem 286:11569-78. 2011..These observations indicate that arfaptins are recruited onto trans-Golgi membranes by interacting with Arl1, and capable of inducing membrane deformation via their BAR domains... - Three homologous ArfGAPs participate in coat protein I-mediated transportAkina Saitoh
Graduate School of Pharmaceutical Sciences and Career Path Promotion Unit for Young Life Scientists, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Biol Chem 284:13948-57. 2009..On the basis of these observations, we conclude that ArfGAP1, ArfGAP2, and ArfGAP3 have overlapping roles in regulating COPI function in Golgi-to-ER retrograde transport... - [GGAs: structure and function of a novel family of clathrin adaptors involved in membrane trafficking between the TGN and endosomes]Kazuhisa Nakayama
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Seikagaku 77:1367-81. 2005 - Functional involvement of TMF/ARA160 in Rab6-dependent retrograde membrane trafficJunko Yamane
Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto, Japan
Exp Cell Res 313:3472-85. 2007..These observations suggest critical roles for TMF in two Rab6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER... - Retroendocytosis pathway of ABCA1/apoA-I contributes to HDL formationYuya Azuma
Kyoto University, Japan
Genes Cells 14:191-204. 2009..These results suggest that the retroendocytosis pathway of ABCA1/apoA-I contributes to HDL formation when excess lipoprotein-derived cholesterol has accumulated in cells... - Restriction of mast cell proliferation through hyaluronan synthesis by co-cultured fibroblastsHirotsugu Takano
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
Biol Pharm Bull 35:408-12. 2012..These findings suggest that the extracellular matrix containing hyaluronan may have a potential to restrict proliferation of cutaneous mast cells in a CD44-independent manner... - Dual control of membrane targeting by PtdIns(4)P and ARFHye-Won Shin
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Trends Biochem Sci 29:513-5. 2004.... - Expression of BIG2 and analysis of its function in mammalian cellsHye-Won Shin
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
Methods Enzymol 404:206-15. 2005..This article describes methods used for examining the function of BIG2 including basic protocols, which are not conventional because of cytotoxicity of BIG2 in Escherichia coli cells and its low expression efficiency in mammalian cells... - Recruitment of clathrin onto endosomes by the Tom1-Tollip complexYohei Katoh
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
Biochem Biophys Res Commun 341:143-9. 2006..These results indicate that, in conjunction with Tollip, Tom1 family proteins play an important role in recruiting clathrin onto endosomes and suggest that they modulate endosomal functions... - ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50 protein-independent mannerHiroyuki Takatsu
Career Path Promotion Unit for Young Life Scientists, Kyoto University, Sakyo ku, Kyoto, Japan
J Biol Chem 286:38159-67. 2011..These results indicate that ATP9B is able to exit the ER and localize to the TGN independently of CDC50 proteins and that this protein contains a Golgi localization signal in its N-terminal cytoplasmic region... - Analysis of Arf interaction with GGAs in vitro and in vivoKazuhisa Nakayama
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
Methods Enzymol 404:367-77. 2005..This chapter describes methods to examine the interaction between Arfs and GGAs and to analyze the cellular function of GGAs regulated by Arfs... - Role of ADP-ribosylation factor 6 (ARF6) in gastric acid secretionJun Matsukawa
Laboratory of Cell Signaling, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113 0033, Japan
J Biol Chem 278:36470-5. 2003..These results suggest that ARF6 regulates gastric acid secretion in parietal cell and that the GTP hydrolysis cycle of ARF6 is essential for the activation pathway... - Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cellsHirotsugu Takano
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
FEBS Lett 582:1444-50. 2008..The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs... - Activation of histidine decarboxylase through post-translational cleavage by caspase-9 in a mouse mastocytoma P-815Kazuyuki Furuta
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Biol Chem 282:13438-46. 2007..These results suggest that, in P-815 cells, histamine synthesis is augmented through the post-translational cleavage of HDC, which is mediated by caspase-9... - Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding abilitySatomi Yogosawa
Department of Neurochemistry, National Institute of Neuroscience, NCNP, Kodaira, Tokyo 187 8502, Japan
Biochem Biophys Res Commun 350:82-90. 2006..These results suggest that the ubiquitin binding and ubiquitylation of GGA3-GAT domain are mutually inseparable through a ubiquitin ligase activity of hVPS18... - Dominant-negative mutant of BIG2, an ARF-guanine nucleotide exchange factor, specifically affects membrane trafficking from the trans-Golgi network through inhibiting membrane association of AP-1 and GGA coat proteinsChisa Shinotsuka
Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
Biochem Biophys Res Commun 294:254-60. 2002..These observations indicate that BIG2 is involved in trafficking from the TGN by regulating membrane association of AP-1 and GGA through activating ARF... - Molecular basis for autoregulatory interaction between GAE domain and hinge region of GGA1Michio Inoue
Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
Traffic 8:904-13. 2007.... - Structural basis for Rab11-dependent membrane recruitment of a family of Rab11-interacting protein 3 (FIP3)/Arfophilin-1Tomoo Shiba
Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Tsukuba, Ibaraki 305 0801, Japan
Proc Natl Acad Sci U S A 103:15416-21. 2006..The bivalent interaction of FIP3 with Rab11 at the C terminus allows FIP3 to coordinately function with other binding partners, including ARFs... - Convergence of cell cycle regulation and growth factor signals on GRASP65Shin ichiro Yoshimura
Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Japan
J Biol Chem 280:23048-56. 2005..The convergence of cell cycle regulation and growth factor signals on GRASP65 Ser-277 suggests that GRASP65 may function as a signal integrator controlling the cell growth... - Membrane recruitment of effector proteins by Arf and Rab GTPasesMasato Kawasaki
Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK, Tsukuba, Ibaraki 305-0801, Japan
Curr Opin Struct Biol 15:681-9. 2005.... - Gamma-adaptin interacts directly with Rabaptin-5 through its ear domainYoko Shiba
Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
J Biochem 131:327-36. 2002..These results suggest that the gamma1-adaptin-Rabaptin-5 interaction may play a role in membrane trafficking between the TGN and endosomes... - GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factorsHiroyuki Takatsu
Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
Biochem J 365:369-78. 2002..In contrast, on the basis of similar experiments, ARF6 (Class III) may be involved in recruitment of GGA proteins to other compartments, possibly early endosomes... - Structural basis for the accessory protein recruitment by the gamma-adaptin ear domainTerukazu Nogi
Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
Nat Struct Biol 9:527-31. 2002.... - GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coatKazumasa Kawamoto
Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
Traffic 3:483-95. 2002..These observations indicate that GBF1 is involved in the formation of COPI-coated vesicles from the cis-Golgi or the pre-Golgi intermediate compartment through activating ADP-ribosylation factors... - Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transportTomoo Shiba
Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
Nat Struct Biol 10:386-93. 2003..These data reveal a molecular mechanism underlying membrane recruitment of adaptor proteins by ARF-GTP... - Predominant expression of the short form of GGA3 in human cell lines and tissuesMasaki Wakasugi
Department of Cell Biology and Neuroscience A1, Osaka University Graduate School of Medicine, Osaka, Japan
Biochem Biophys Res Commun 306:687-92. 2003..These results indicate that GGA3-S which would not be directly involved in the cargo protein recognition is predominantly expressed in human tissues except the brain and in cell lines... - GAT (GGA and Tom1) domain responsible for ubiquitin binding and ubiquitinationYoko Shiba
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
J Biol Chem 279:7105-11. 2004.... - Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ERAbbas Shakoori
Division of Molecular Membrane Biology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan
Biochem Biophys Res Commun 312:850-7. 2003..These results suggest that FinGERs function in maintenance of the Golgi structure and/or transport between the ER and the Golgi apparatus... - Tollip and Tom1 form a complex and recruit ubiquitin-conjugated proteins onto early endosomesYohei Katoh
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8572, Japan
J Biol Chem 279:24435-43. 2004..On the other hand, Tollip was localized on early endosomes and recruited Tom1 and ubiquitinated proteins. These observations suggest that Tollip and Tom1 form a complex and regulate endosomal trafficking of ubiquitinated proteins... - Stabilin-1 localizes to endosomes and the trans-Golgi network in human macrophages and interacts with GGA adaptorsJulia Kzhyshkowska
Department of Dermatology, University Medical Centre Mannheim, University of Heidelberg, Theodor Kutzer Ufer 1 3, 68167 Mannheim, Germany
J Leukoc Biol 76:1151-61. 2004.... - BIG2, a guanine nucleotide exchange factor for ADP-ribosylation factors: its localization to recycling endosomes and implication in the endosome integrityHye-Won Shin
Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa 920-0934, Japan
Mol Biol Cell 15:5283-94. 2004..These observations together indicate that BIG2 is implicated in the structural integrity of the recycling endosome through activating class I ARFs... - Structure determination of GGA-GAE and gamma1-ear in complex with peptides: crystallization of low-affinity complexes in membrane trafficYusuke Yamada
Structural Biology Research Center, Photon Factory, High Energy Accelerator Research Organization, Japan
Acta Crystallogr D Biol Crystallogr 61:731-6. 2005..The peptide-tagged gamma1-ear readily crystallized and the crystal diffracted far better, 1.9-2.2 A resolution, compared with the co-crystallized complex, giving significantly more details without affecting the overall complex structure... - Molecular mechanism of ubiquitin recognition by GGA3 GAT domainMasato Kawasaki
Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK, Tsukuba, Ibaraki 305-0801, Japan
Genes Cells 10:639-54. 2005..The double-sided ubiquitin binding provides the GAT domain with higher efficiency in recognizing ubiquitinated receptors for lysosomal receptor degradation... - Structural basis for recognition of acidic-cluster dileucine sequence by GGA1Tomoo Shiba
Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
Nature 415:937-41. 2002..This recognition mechanism provides the basis for regulation of protein transport from the TGN to endosomes/lysosomes, which is shared by sortilin and low-density lipoprotein receptor-related protein... - Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cellsShigetsune Matsuya
Division of Histology, Department of Cell Biology, Tohoku University Graduate School of Medicine, Sendai, Japan
J Neurochem 93:674-85. 2005..These findings suggest that EFA6C may be involved in the regulation of the membrane dynamics of the somatodendritic compartments of Purkinje cells through the activation of ARF6... - Structural basis for recognition of ubiquitinated cargo by Tom1-GAT domainMasato Akutsu
Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK, Tsukuba, Ibaraki, Japan
FEBS Lett 579:5385-91. 2005..The double-sided ubiquitin binding enhances the efficiency of recognition of ubiquitinated proteins by Tom1...