Masato Nakagawa

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. doi request reprint Reprogramming of somatic cells to pluripotency
    Masato Nakagawa
    Center for iPS Research and Application Institute for Integrated Cell Material Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, 606 8507, Japan
    Adv Exp Med Biol 695:215-24. 2010
  2. pmc Promotion of direct reprogramming by transformation-deficient Myc
    Masato Nakagawa
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 107:14152-7. 2010
  3. doi request reprint Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
  4. pmc Suppression of induced pluripotent stem cell generation by the p53-p21 pathway
    Hyenjong Hong
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 460:1132-5. 2009
  5. doi request reprint Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblasts
    Noriko Tsubooka
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Genes Cells 14:683-94. 2009
  6. pmc Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells
    Masanori Imamura
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    BMC Dev Biol 6:34. 2006
  7. doi request reprint Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
  8. doi request reprint Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
    Shogo Nagata
    Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Genes Cells 14:1395-404. 2009
  9. doi request reprint A more efficient method to generate integration-free human iPS cells
    Keisuke Okita
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    Nat Methods 8:409-12. 2011
  10. doi request reprint Human and mouse induced pluripotent stem cells are differentially reprogrammed in response to kinase inhibitors
    Kunio Hirano
    Laboratory of Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Stem Cells Dev 21:1287-98. 2012

Collaborators

Detail Information

Publications30

  1. doi request reprint Reprogramming of somatic cells to pluripotency
    Masato Nakagawa
    Center for iPS Research and Application Institute for Integrated Cell Material Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, 606 8507, Japan
    Adv Exp Med Biol 695:215-24. 2010
    ....
  2. pmc Promotion of direct reprogramming by transformation-deficient Myc
    Masato Nakagawa
    Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 107:14152-7. 2010
    ..These data demonstrate that different functional moieties of the Myc proto-oncogene products are involved in the transformation and promotion of directed reprogramming...
  3. doi request reprint Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
    ..The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine...
  4. pmc Suppression of induced pluripotent stem cell generation by the p53-p21 pathway
    Hyenjong Hong
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nature 460:1132-5. 2009
    ..Functional analyses of these genes demonstrate that the p53-p21 pathway serves as a barrier not only in tumorigenicity, but also in iPS cell generation...
  5. doi request reprint Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblasts
    Noriko Tsubooka
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Genes Cells 14:683-94. 2009
    ..These results demonstrated that Sall4 plays positive roles in the generation of pluripotent stem cells from blastocysts and fibroblasts...
  6. pmc Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells
    Masanori Imamura
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    BMC Dev Biol 6:34. 2006
    ..We previously identified a set of genes called ECATs (ES cell-associated transcripts) that are expressed at high levels in mouse ES cells. Here, we examine the expression and DNA methylation of ECATs in somatic cells and germ cells...
  7. doi request reprint Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
    ..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required...
  8. doi request reprint Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cells
    Shogo Nagata
    Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Genes Cells 14:1395-404. 2009
    ....
  9. doi request reprint A more efficient method to generate integration-free human iPS cells
    Keisuke Okita
    Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    Nat Methods 8:409-12. 2011
    ..This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future...
  10. doi request reprint Human and mouse induced pluripotent stem cells are differentially reprogrammed in response to kinase inhibitors
    Kunio Hirano
    Laboratory of Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Stem Cells Dev 21:1287-98. 2012
    ..Thus, 2i-hiPSCs, which are nonteratoma forming PNSCs, may represent a safe source of cells for neural research and regenerative medicine...
  11. ncbi request reprint Induction of pluripotent stem cells from fibroblast cultures
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara machi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Protoc 2:3081-9. 2007
    ..The cells, named induced pluripotent stem (iPS) cells, can be differentiated into three germ layers and committed to chimeric mice. Here we describe detailed methods and tips for the generation of iPS cells...
  12. ncbi request reprint Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
    Masato Nakagawa
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Nat Biotechnol 26:101-6. 2008
    ..The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC...
  13. doi request reprint Variation in the safety of induced pluripotent stem cell lines
    Kyoko Miura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
    Nat Biotechnol 27:743-5. 2009
    ..In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells...
  14. pmc A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
    Masato Nakagawa
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Sci Rep 4:3594. 2014
    ..These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system. ..
  15. doi request reprint X-linked severe combined immunodeficiency (X-SCID) rats for xeno-transplantation and behavioral evaluation
    Bumpei Samata
    Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
    J Neurosci Methods 243:68-77. 2015
    ..In the case of such xeno-transplantation, however, immunosuppression is needed for good survival of the grafted cells...
  16. pmc Essential roles of ECAT15-2/Dppa2 in functional lung development
    Tomonori Nakamura
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
    Mol Cell Biol 31:4366-78. 2011
    ..These data suggest that ECAT15-2 has important roles in lung development, where it is no longer expressed, by leaving epigenetic marks from earlier developmental stages...
  17. doi request reprint Orderly hematopoietic development of induced pluripotent stem cells via Flk-1(+) hemoangiogenic progenitors
    Akira Niwa
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    J Cell Physiol 221:367-77. 2009
    ..This finding highlights the potential use of iPS cells in clinical areas such as regenerative medicine, disease investigation, and drug screening...
  18. doi request reprint Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells
    Kotaro Sasaki
    Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan JST, ERATO, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Cell Stem Cell 17:178-94. 2015
    ..Together, these findings provide a foundation for understanding and reconstituting human germ cell development in vitro...
  19. pmc Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5)
    Hisayuki Amano
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    BMC Dev Biol 6:11. 2006
    ..Previous studies identified genomic clones containing the mouse ESG1 gene and five pseudogenes. However, their chromosomal localizations or physiological functions have not been determined...
  20. ncbi request reprint Genetic diversity of fragmented populations of Polygala reinii (Polygalaceae), a perennial herb endemic to Japan
    Masato Nakagawa
    Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    J Plant Res 117:355-61. 2004
    ..On the other hand, the geographical differentiation between the Japan Sea and Pacific Ocean sides was found in P. reinii, suggesting the influence of postglacial migration on the establishment of the genetic structure of this species...
  21. doi request reprint Fine-scale genetic structure within plots of Polygala reinii (Polygalaceae) having an ant-dispersal seed
    Masato Nakagawa
    The Kyoto University Museum, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8502, Japan
    J Plant Res 123:355-62. 2010
    ..reinii...
  22. ncbi request reprint Ploidy, geographical distribution and morphological differentiation of Parasenecio auriculata (Senecioneae; Asteraceae) in Japan
    Masato Nakagawa
    Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606 8502, Japan
    J Plant Res 119:51-61. 2006
    ..Such a geographical pattern is possibly attributable to the differentiation of climatic preference among cytotypes and varieties, and may have been established in association with the climatic cline along the Japanese archipelago...
  23. pmc SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression
    Tomonori Nakamura
    Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan JST, ERATO, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Nucleic Acids Res 43:e60. 2015
    ..We propose that SC3-seq might be used as a powerful strategy for single-cell transcriptome analysis in a broad range of investigations in biomedical sciences. ..
  24. pmc Isolation of human induced pluripotent stem cell-derived dopaminergic progenitors by cell sorting for successful transplantation
    Daisuke Doi
    Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, 606 8507 Kyoto, Japan
    Stem Cell Reports 2:337-50. 2014
    ..Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application. ..
  25. ncbi request reprint Differential membrane localization of ERas and Rheb, two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University and CREST, Japan Science and Technology Agency, Kyoto 606 8507, Japan
    J Biol Chem 280:32768-74. 2005
    ..Rheb also shares the same membrane-targeting pathway but because of the absence of palmitoylation is located on endomembranes...
  26. pmc Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-A
    Masataka Fujiwara
    Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e16734. 2011
    ..These results provide a technological basis to obtain functional cardiomyocytes from iPSCs...
  27. pmc Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media
    Makoto Fukuta
    Department of Tissue Regeneration, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan
    PLoS ONE 9:e112291. 2014
    ..Our simple and robust induction protocol using small molecule compounds with defined media enabled the generation of hNCCs as an intermediate material producing terminally differentiated cells for cell-based innovative medicine. ..
  28. doi request reprint Rapid and deep profiling of human induced pluripotent stem cell proteome by one-shot NanoLC-MS/MS analysis with meter-scale monolithic silica columns
    Ryota Yamana
    Department of Molecular and Cellular BioAnalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Proteome Res 12:214-21. 2013
    ..Our results indicate that this simplified one-shot proteomics approach with long monolithic columns is advantageous for rapid, deep, sensitive, and reproducible proteome analysis...
  29. ncbi request reprint Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells
    Masayoshi Maruyama
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 280:24371-9. 2005
    ..Taken together, these data demonstrate differential roles for Sox15 and Sox2 in transcriptional control in mouse ES cells...
  30. doi request reprint Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons
    Shin Il Kim
    Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, 606 8507, Japan
    Methods Mol Biol 1357:111-31. 2016
    ..In this chapter, we outline the features of a panel of "All-in-One" PB transposons designed for drug-inducible gene expression and provide guidelines to establish and validate populations or clones of transgenic hiPSCs. ..