Research Topics
Genomes and Genes
| Masato NakagawaSummaryAffiliation: Kyoto University Country: Japan Publications
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Detail Information
Publications
Promotion of direct reprogramming by transformation-deficient MycMasato Nakagawa
Center for iPS Cell Research and Application, Kyoto University, Kyoto 606 8507, Japan
Proc Natl Acad Sci U S A 107:14152-7. 2010..These data demonstrate that different functional moieties of the Myc proto-oncogene products are involved in the transformation and promotion of directed reprogramming...
Reprogramming of somatic cells to pluripotencyMasato Nakagawa
Center for iPS Research and Application Institute for Integrated Cell Material Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, 606 8507, Japan
Adv Exp Med Biol 695:215-24. 2010....- Suppression of induced pluripotent stem cell generation by the p53-p21 pathwayHyenjong Hong
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
Nature 460:1132-5. 2009..Functional analyses of these genes demonstrate that the p53-p21 pathway serves as a barrier not only in tumorigenicity, but also in iPS cell generation... - Generation of mouse induced pluripotent stem cells without viral vectorsKeisuke Okita
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
Science 322:949-53. 2008..The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine... - Roles of Sall4 in the generation of pluripotent stem cells from blastocysts and fibroblastsNoriko Tsubooka
Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
Genes Cells 14:683-94. 2009..These results demonstrated that Sall4 plays positive roles in the generation of pluripotent stem cells from blastocysts and fibroblasts... - Generation of pluripotent stem cells from adult mouse liver and stomach cellsTakashi Aoi
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
Science 321:699-702. 2008..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required... - Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cellsMasanori Imamura
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
BMC Dev Biol 6:34. 2006..We previously identified a set of genes called ECATs (ES cell-associated transcripts) that are expressed at high levels in mouse ES cells. Here, we examine the expression and DNA methylation of ECATs in somatic cells and germ cells... - Efficient reprogramming of human and mouse primary extra-embryonic cells to pluripotent stem cellsShogo Nagata
Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
Genes Cells 14:1395-404. 2009.... - A more efficient method to generate integration-free human iPS cellsKeisuke Okita
Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
Nat Methods 8:409-12. 2011..This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future... - Human and mouse induced pluripotent stem cells are differentially reprogrammed in response to kinase inhibitorsKunio Hirano
Laboratory of Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Stem Cells Dev 21:1287-98. 2012..Thus, 2i-hiPSCs, which are nonteratoma forming PNSCs, may represent a safe source of cells for neural research and regenerative medicine... - Variation in the safety of induced pluripotent stem cell linesKyoko Miura
Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto, Japan
Nat Biotechnol 27:743-5. 2009..In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells... 
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblastsMasato Nakagawa
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
Nat Biotechnol 26:101-6. 2008..The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC...- Induction of pluripotent stem cells from fibroblast culturesKazutoshi Takahashi
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara machi, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
Nat Protoc 2:3081-9. 2007..The cells, named induced pluripotent stem (iPS) cells, can be differentiated into three germ layers and committed to chimeric mice. Here we describe detailed methods and tips for the generation of iPS cells... - Essential roles of ECAT15-2/Dppa2 in functional lung developmentTomonori Nakamura
Center for iPS Cell Research and Application CiRA, Kyoto University, Kyoto 606 8507, Japan
Mol Cell Biol 31:4366-78. 2011..These data suggest that ECAT15-2 has important roles in lung development, where it is no longer expressed, by leaving epigenetic marks from earlier developmental stages... - Orderly hematopoietic development of induced pluripotent stem cells via Flk-1(+) hemoangiogenic progenitorsAkira Niwa
Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
J Cell Physiol 221:367-77. 2009..This finding highlights the potential use of iPS cells in clinical areas such as regenerative medicine, disease investigation, and drug screening... - Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5)Hisayuki Amano
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
BMC Dev Biol 6:11. 2006..Previous studies identified genomic clones containing the mouse ESG1 gene and five pseudogenes. However, their chromosomal localizations or physiological functions have not been determined... - Fine-scale genetic structure within plots of Polygala reinii (Polygalaceae) having an ant-dispersal seedMasato Nakagawa
The Kyoto University Museum, Kyoto University, Yoshida Honmachi, Sakyo ku, Kyoto 606 8502, Japan
J Plant Res 123:355-62. 2010..reinii... - Genetic diversity of fragmented populations of Polygala reinii (Polygalaceae), a perennial herb endemic to JapanMasato Nakagawa
Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
J Plant Res 117:355-61. 2004..On the other hand, the geographical differentiation between the Japan Sea and Pacific Ocean sides was found in P. reinii, suggesting the influence of postglacial migration on the establishment of the genetic structure of this species... - Ploidy, geographical distribution and morphological differentiation of Parasenecio auriculata (Senecioneae; Asteraceae) in JapanMasato Nakagawa
Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606 8502, Japan
J Plant Res 119:51-61. 2006..Such a geographical pattern is possibly attributable to the differentiation of climatic preference among cytotypes and varieties, and may have been established in association with the climatic cline along the Japanese archipelago... - Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-AMasataka Fujiwara
Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
PLoS ONE 6:e16734. 2011..These results provide a technological basis to obtain functional cardiomyocytes from iPSCs... - Differential membrane localization of ERas and Rheb, two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathwayKazutoshi Takahashi
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University and CREST, Japan Science and Technology Agency, Kyoto 606 8507, Japan
J Biol Chem 280:32768-74. 2005..Rheb also shares the same membrane-targeting pathway but because of the absence of palmitoylation is located on endomembranes... - Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cellsMasayoshi Maruyama
Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
J Biol Chem 280:24371-9. 2005..Taken together, these data demonstrate differential roles for Sox15 and Sox2 in transcriptional control in mouse ES cells... - Rapid and deep profiling of human induced pluripotent stem cell proteome by one-shot NanoLC-MS/MS analysis with meter-scale monolithic silica columnsRyota Yamana
Department of Molecular and Cellular BioAnalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Proteome Res 12:214-21. 2013..Our results indicate that this simplified one-shot proteomics approach with long monolithic columns is advantageous for rapid, deep, sensitive, and reproducible proteome analysis...