Kimihiko Mizutani

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Structure of aluminium-bound ovotransferrin at 2.15 Angstroms resolution
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Japan
    Acta Crystallogr D Biol Crystallogr 61:1636-42. 2005
  2. pmc X-ray analysis of bilirubin oxidase from Myrothecium verrucaria at 2.3 A resolution using a twinned crystal
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:765-70. 2010
  3. doi request reprint Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris
    Kimihiko Mizutani
    Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Biochim Biophys Acta 1824:954-62. 2012
  4. doi request reprint X-ray structures of transferrins and related proteins
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochim Biophys Acta 1820:203-11. 2012
  5. ncbi request reprint Thermostabilization of ovotransferrin by anions for pasteurization of liquid egg white
    Kimihiko Mizutani
    Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Biosci Biotechnol Biochem 70:1839-45. 2006
  6. ncbi request reprint Structural and functional characterization of recombinant human serum transferrin secreted from Pichia pastoris
    Kimihiko Mizutani
    Graduate School of Agriculture, Kyoto University, Uji, Japan
    Biosci Biotechnol Biochem 74:309-15. 2010
  7. doi request reprint High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Protein Expr Purif 77:1-8. 2011
  8. ncbi request reprint Iron-binding process in the amino- and carboxyl-terminal lobes of ovotransferrin: quantitative studies utilizing single Fe3+-binding mutants
    Ikuko Okamoto
    Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 43:11118-25. 2004
  9. ncbi request reprint Structural and functional characterization of ovotransferrin produced by Pichia pastoris
    Kimihiko Mizutani
    The Graduate School of Agriculture, Kyoto University, Japan
    Biosci Biotechnol Biochem 68:376-83. 2004
  10. pmc Crystal structures of protein glutaminase and its pro forms converted into enzyme-substrate complex
    Ryota Hashizume
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611 0011, Japan
    J Biol Chem 286:38691-702. 2011

Collaborators

Detail Information

Publications11

  1. ncbi request reprint Structure of aluminium-bound ovotransferrin at 2.15 Angstroms resolution
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Japan
    Acta Crystallogr D Biol Crystallogr 61:1636-42. 2005
    ..These findings support the participation of transferrin in the transport of Al(3+) ions in vivo...
  2. pmc X-ray analysis of bilirubin oxidase from Myrothecium verrucaria at 2.3 A resolution using a twinned crystal
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:765-70. 2010
    ....
  3. doi request reprint Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris
    Kimihiko Mizutani
    Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Biochim Biophys Acta 1824:954-62. 2012
    ..Using this plasmid, high-throughput construction of an expression system by homologous recombination in P. pastoris cells, previously reported for membrane proteins, was successfully applied to the secretory protein...
  4. doi request reprint X-ray structures of transferrins and related proteins
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochim Biophys Acta 1820:203-11. 2012
    ..Transferrins are a group of iron-binding proteins including serum transferrin, lactoferrin and ovotransferrin...
  5. ncbi request reprint Thermostabilization of ovotransferrin by anions for pasteurization of liquid egg white
    Kimihiko Mizutani
    Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Biosci Biotechnol Biochem 70:1839-45. 2006
    ..These binding sites may have been concerned with sulfate binding (not bicarbonate binding) that is found in the crystal structure of apo-form of oTf, since the bicarbonate anion did not raise T(m)...
  6. ncbi request reprint Structural and functional characterization of recombinant human serum transferrin secreted from Pichia pastoris
    Kimihiko Mizutani
    Graduate School of Agriculture, Kyoto University, Uji, Japan
    Biosci Biotechnol Biochem 74:309-15. 2010
    ....
  7. doi request reprint High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Protein Expr Purif 77:1-8. 2011
    ..cerevisiae) were constructed, and the expression of proteins was analyzed by fluorescence spectra, fluorescence microscopy, and SDS-PAGE (in-gel fluorescence detection)...
  8. ncbi request reprint Iron-binding process in the amino- and carboxyl-terminal lobes of ovotransferrin: quantitative studies utilizing single Fe3+-binding mutants
    Ikuko Okamoto
    Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 43:11118-25. 2004
    ..25 kcal/mol) and upon the secondary C-lobe iron binding in the monoferric N-lobe (DeltaG(AB), -6.45 kcal/mol)...
  9. ncbi request reprint Structural and functional characterization of ovotransferrin produced by Pichia pastoris
    Kimihiko Mizutani
    The Graduate School of Agriculture, Kyoto University, Japan
    Biosci Biotechnol Biochem 68:376-83. 2004
    ....
  10. pmc Crystal structures of protein glutaminase and its pro forms converted into enzyme-substrate complex
    Ryota Hashizume
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611 0011, Japan
    J Biol Chem 286:38691-702. 2011
    ..5 Å resolution were found to correspond to the enzyme-substrate complex and an S-acyl intermediate. Based on these structures, the catalytic mechanism of protein glutaminase is proposed...
  11. pmc Structure of β-1,4-mannanase from the common sea hare Aplysia kurodai at 1.05 Å resolution
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1164-8. 2012
    ....