Kimihiko Mizutani

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi Structure of aluminium-bound ovotransferrin at 2.15 Angstroms resolution
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Japan
    Acta Crystallogr D Biol Crystallogr 61:1636-42. 2005
  2. ncbi X-ray analysis of bilirubin oxidase from Myrothecium verrucaria at 2.3 A resolution using a twinned crystal
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:765-70. 2010
  3. ncbi X-ray structures of transferrins and related proteins
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochim Biophys Acta 1820:203-11. 2012
  4. ncbi Thermostabilization of ovotransferrin by anions for pasteurization of liquid egg white
    Kimihiko Mizutani
    Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Biosci Biotechnol Biochem 70:1839-45. 2006
  5. ncbi Structural and functional characterization of recombinant human serum transferrin secreted from Pichia pastoris
    Kimihiko Mizutani
    Graduate School of Agriculture, Kyoto University, Uji, Japan
    Biosci Biotechnol Biochem 74:309-15. 2010
  6. ncbi High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Protein Expr Purif 77:1-8. 2011
  7. ncbi Iron-binding process in the amino- and carboxyl-terminal lobes of ovotransferrin: quantitative studies utilizing single Fe3+-binding mutants
    Ikuko Okamoto
    Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 43:11118-25. 2004
  8. ncbi Structural and functional characterization of ovotransferrin produced by Pichia pastoris
    Kimihiko Mizutani
    The Graduate School of Agriculture, Kyoto University, Japan
    Biosci Biotechnol Biochem 68:376-83. 2004
  9. ncbi Crystal structures of protein glutaminase and its pro forms converted into enzyme-substrate complex
    Ryota Hashizume
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611 0011, Japan
    J Biol Chem 286:38691-702. 2011
  10. ncbi Structure of β-1,4-mannanase from the common sea hare Aplysia kurodai at 1.05 Å resolution
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1164-8. 2012

Collaborators

Detail Information

Publications10

  1. ncbi Structure of aluminium-bound ovotransferrin at 2.15 Angstroms resolution
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Japan
    Acta Crystallogr D Biol Crystallogr 61:1636-42. 2005
    ..These findings support the participation of transferrin in the transport of Al(3+) ions in vivo...
  2. ncbi X-ray analysis of bilirubin oxidase from Myrothecium verrucaria at 2.3 A resolution using a twinned crystal
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:765-70. 2010
    ....
  3. ncbi X-ray structures of transferrins and related proteins
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochim Biophys Acta 1820:203-11. 2012
    ..Transferrins are a group of iron-binding proteins including serum transferrin, lactoferrin and ovotransferrin...
  4. ncbi Thermostabilization of ovotransferrin by anions for pasteurization of liquid egg white
    Kimihiko Mizutani
    Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Biosci Biotechnol Biochem 70:1839-45. 2006
    ..These binding sites may have been concerned with sulfate binding (not bicarbonate binding) that is found in the crystal structure of apo-form of oTf, since the bicarbonate anion did not raise T(m)...
  5. ncbi Structural and functional characterization of recombinant human serum transferrin secreted from Pichia pastoris
    Kimihiko Mizutani
    Graduate School of Agriculture, Kyoto University, Uji, Japan
    Biosci Biotechnol Biochem 74:309-15. 2010
    ....
  6. ncbi High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Protein Expr Purif 77:1-8. 2011
    ..cerevisiae) were constructed, and the expression of proteins was analyzed by fluorescence spectra, fluorescence microscopy, and SDS-PAGE (in-gel fluorescence detection)...
  7. ncbi Iron-binding process in the amino- and carboxyl-terminal lobes of ovotransferrin: quantitative studies utilizing single Fe3+-binding mutants
    Ikuko Okamoto
    Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 43:11118-25. 2004
    ..25 kcal/mol) and upon the secondary C-lobe iron binding in the monoferric N-lobe (DeltaG(AB), -6.45 kcal/mol)...
  8. ncbi Structural and functional characterization of ovotransferrin produced by Pichia pastoris
    Kimihiko Mizutani
    The Graduate School of Agriculture, Kyoto University, Japan
    Biosci Biotechnol Biochem 68:376-83. 2004
    ....
  9. ncbi Crystal structures of protein glutaminase and its pro forms converted into enzyme-substrate complex
    Ryota Hashizume
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611 0011, Japan
    J Biol Chem 286:38691-702. 2011
    ..5 Å resolution were found to correspond to the enzyme-substrate complex and an S-acyl intermediate. Based on these structures, the catalytic mechanism of protein glutaminase is proposed...
  10. ncbi Structure of β-1,4-mannanase from the common sea hare Aplysia kurodai at 1.05 Å resolution
    Kimihiko Mizutani
    Laboratory of Applied Structural Biology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1164-8. 2012
    ....