Kei Miyamoto

Summary

Affiliation: University of Cambridge
Location: Cambridge, UK

Publications

  1. ncbi request reprint Effects of synchronization of donor cell cycle on embryonic development and DNA synthesis in porcine nuclear transfer embryos
    Kei Miyamoto
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    J Reprod Dev 53:237-46. 2007
  2. doi request reprint Epigenetic factors influencing resistance to nuclear reprogramming
    Vincent Pasque
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Trends Genet 27:516-25. 2011
  3. pmc Mechanisms of nuclear reprogramming by eggs and oocytes: a deterministic process?
    Jerome Jullien
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Rev Mol Cell Biol 12:453-9. 2011
  4. pmc Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes
    Kei Miyamoto
    Wellcome Trust, Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom
    Genes Dev 25:946-58. 2011
  5. pmc Nuclear actin and transcriptional activation
    Kei Miyamoto
    Wellcome Trust Cancer Research UK Gurdon Institute University of Cambridge Tennis Court Road, Cambridge UK
    Commun Integr Biol 4:582-3. 2011
  6. doi request reprint Efficiencies and mechanisms of nuclear reprogramming
    V Pasque
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Cambridge CB2 1QN, United Kingdom
    Cold Spring Harb Symp Quant Biol 75:189-200. 2010
  7. doi request reprint Cell-free extracts from mammalian oocytes partially induce nuclear reprogramming in somatic cells
    Kei Miyamoto
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto, Japan
    Biol Reprod 80:935-43. 2009
  8. doi request reprint Reversible membrane permeabilization of mammalian cells treated with digitonin and its use for inducing nuclear reprogramming by Xenopus egg extracts
    Kei Miyamoto
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto 606 8502, Japan
    Cloning Stem Cells 10:535-42. 2008
  9. ncbi request reprint Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts
    Kei Miyamoto
    Laboratory of Reproductive Biology, Kyoto University, Kyoto, Japan
    Mol Reprod Dev 74:1268-77. 2007
  10. pmc Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos
    Kei Miyamoto
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 108:7040-5. 2011

Detail Information

Publications10

  1. ncbi request reprint Effects of synchronization of donor cell cycle on embryonic development and DNA synthesis in porcine nuclear transfer embryos
    Kei Miyamoto
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    J Reprod Dev 53:237-46. 2007
    ..The SCNT system using G1-synchronized cells, in terms of their highly uniform and viable cell states, can be useful for studying the reprogramming processes and embryonic development of SCNT embryos...
  2. doi request reprint Epigenetic factors influencing resistance to nuclear reprogramming
    Vincent Pasque
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Trends Genet 27:516-25. 2011
    ..We see this as a step towards understanding the mechanisms by which nuclear reprogramming takes place...
  3. pmc Mechanisms of nuclear reprogramming by eggs and oocytes: a deterministic process?
    Jerome Jullien
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Rev Mol Cell Biol 12:453-9. 2011
    ..These events occur in an ordered manner and on a defined timescale, indicating that reprogramming by nuclear transfer and by cell fusion rely on deterministic processes...
  4. pmc Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes
    Kei Miyamoto
    Wellcome Trust, Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom
    Genes Dev 25:946-58. 2011
    ..This is the first report showing that naturally stored actin in an oocyte nucleus helps transcriptional reprogramming in a polymerization-dependent manner...
  5. pmc Nuclear actin and transcriptional activation
    Kei Miyamoto
    Wellcome Trust Cancer Research UK Gurdon Institute University of Cambridge Tennis Court Road, Cambridge UK
    Commun Integr Biol 4:582-3. 2011
    ..This study emphasizes a significant contribution of nuclear actin in transcriptional activation. Here, we discuss possible roles of nuclear actin in Xenopus oocytes and in other cell types in the context of transcriptional activation...
  6. doi request reprint Efficiencies and mechanisms of nuclear reprogramming
    V Pasque
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, Cambridge CB2 1QN, United Kingdom
    Cold Spring Harb Symp Quant Biol 75:189-200. 2010
    ..We comment on the mechanisms that lead to successful somatic-cell reprogramming and on those that resist in helping to maintain the differentiated state of somatic cells...
  7. doi request reprint Cell-free extracts from mammalian oocytes partially induce nuclear reprogramming in somatic cells
    Kei Miyamoto
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto, Japan
    Biol Reprod 80:935-43. 2009
    ..These results indicate that cell-free extracts derived from GV- and MII-oocytes could be useful for studying the mechanisms involved in nuclear reprogramming...
  8. doi request reprint Reversible membrane permeabilization of mammalian cells treated with digitonin and its use for inducing nuclear reprogramming by Xenopus egg extracts
    Kei Miyamoto
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto 606 8502, Japan
    Cloning Stem Cells 10:535-42. 2008
    ..Our results suggest that reversible permeabilization with digitonin can be used to induce nuclear reprogramming and to activate pluripotent genes by a cell-free system...
  9. ncbi request reprint Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts
    Kei Miyamoto
    Laboratory of Reproductive Biology, Kyoto University, Kyoto, Japan
    Mol Reprod Dev 74:1268-77. 2007
    ..A system using egg extracts may be useful for understanding the mechanisms and processes of dedifferentiation and reprogramming of mammalian somatic cells after nuclear transfer...
  10. pmc Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos
    Kei Miyamoto
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 108:7040-5. 2011
    ..This study presents a means for identifying natural reprogramming factors in mammalian oocytes and a unique insight into the mechanisms underlying reprogramming by nuclear transfer...