K Matsuzaki

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Interaction between amyloid beta-protein aggregates and membranes
    Atsuko Kakio
    Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pept Sci 10:612-21. 2004
  2. ncbi request reprint "Click peptide" based on the "o-acyl isopeptide method": control of A beta1-42 production from a photo-triggered A beta1-42 analogue
    Atsuhiko Taniguchi
    Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, Kyoto Pharmaceutical University, Yamashina ku, Kyoto 607 8412, Japan
    J Am Chem Soc 128:696-7. 2006
  3. pmc Formation of Toxic Amyloid Fibrils by Amyloid β-Protein on Ganglioside Clusters
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Int J Alzheimers Dis 2011:956104. 2011
  4. ncbi request reprint Magainins as paradigm for the mode of action of pore forming polypeptides
    K Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1376:391-400. 1998
  5. ncbi request reprint Why and how are peptide-lipid interactions utilized for self defence?
    K Matsuzaki
    Kyoto University, Graduate School of Biostudies, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Soc Trans 29:598-601. 2001
  6. ncbi request reprint [Ganglioside cluster-mediated aggregation and cytotoxicity of amyloid beta-peptide: molecular mechanism and inhibition]
    Katsumi Matsuzaki
    Kyoto University, Kyoto, Japan
    Yakugaku Zasshi 130:511-5. 2010
  7. ncbi request reprint [Interactions between amyloid beta--protein and gangliosides]
    Katsumi Matsuzaki
    Tanpakushitsu Kakusan Koso 47:351-6. 2002
  8. doi request reprint Control of cell selectivity of antimicrobial peptides
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1788:1687-92. 2009
  9. doi request reprint Design, synthesis, and biophysical properties of a helical Abeta1-42 analog: Inhibition of fibrillogenesis and cytotoxicity
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Biophys Res Commun 371:777-80. 2008
  10. ncbi request reprint Physicochemical interactions of amyloid beta-peptide with lipid bilayers
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1768:1935-42. 2007

Collaborators

Detail Information

Publications63

  1. ncbi request reprint Interaction between amyloid beta-protein aggregates and membranes
    Atsuko Kakio
    Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pept Sci 10:612-21. 2004
    ..Fluorescence experiments revealed that Abeta aggregated in buffer did not show any affinity for membranes...
  2. ncbi request reprint "Click peptide" based on the "o-acyl isopeptide method": control of A beta1-42 production from a photo-triggered A beta1-42 analogue
    Atsuhiko Taniguchi
    Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, Kyoto Pharmaceutical University, Yamashina ku, Kyoto 607 8412, Japan
    J Am Chem Soc 128:696-7. 2006
    ..This method provides a novel system useful for investigating the dynamic biological functions of Abeta1-42 in AD by inducible activation of Abeta1-42 self-assembly...
  3. pmc Formation of Toxic Amyloid Fibrils by Amyloid β-Protein on Ganglioside Clusters
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Int J Alzheimers Dis 2011:956104. 2011
    ..Furthermore, differences between Aβ-(1-40) and Aβ-(1-42) in membrane interaction and amyloidogenesis are also emphasized...
  4. ncbi request reprint Magainins as paradigm for the mode of action of pore forming polypeptides
    K Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1376:391-400. 1998
    ..A novel mode of action is discussed in detail, i.e., the formation of a dynamic peptide-lipid supramolecular pore, which allows the mutually coupled transbilayer transport of ions, lipids, and peptides per se...
  5. ncbi request reprint Why and how are peptide-lipid interactions utilized for self defence?
    K Matsuzaki
    Kyoto University, Graduate School of Biostudies, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Soc Trans 29:598-601. 2001
    ..The peptide molecule is internalized upon the disintegration of the pore. Several anti-microbial peptides are known to work synergistically...
  6. ncbi request reprint [Ganglioside cluster-mediated aggregation and cytotoxicity of amyloid beta-peptide: molecular mechanism and inhibition]
    Katsumi Matsuzaki
    Kyoto University, Kyoto, Japan
    Yakugaku Zasshi 130:511-5. 2010
    ..Furthermore, nordihydroguaiaretic acid was found to effectively inhibit the ganglioside-induced amyloidogenesis by preventing the binding of Abeta to the membrane...
  7. ncbi request reprint [Interactions between amyloid beta--protein and gangliosides]
    Katsumi Matsuzaki
    Tanpakushitsu Kakusan Koso 47:351-6. 2002
  8. doi request reprint Control of cell selectivity of antimicrobial peptides
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1788:1687-92. 2009
    ..Pros and cons of these approaches are discussed...
  9. doi request reprint Design, synthesis, and biophysical properties of a helical Abeta1-42 analog: Inhibition of fibrillogenesis and cytotoxicity
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Biophys Res Commun 371:777-80. 2008
    ..Thus, our helical Abeta1-42 is not only a model peptide to investigate the role of helical intermediates in fibrillization by Abeta, but also an inhibitor of Abeta-induced cytotoxicity...
  10. ncbi request reprint Physicochemical interactions of amyloid beta-peptide with lipid bilayers
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1768:1935-42. 2007
    ..The importance of ganglioside clusters in the aggregation of Abeta is emphasized. Finally, a hypothetical physicochemical cascade in the pathogenesis of the disease is proposed...
  11. ncbi request reprint Inhibitors of amyloid beta-protein aggregation mediated by GM1-containing raft-like membranes
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1768:122-30. 2007
    ..Furthermore, a comparison of the inhibitory effects of various compounds between aqueous-phase and GM1-mediated aggregation of Abeta suggested that the two aggregation processes are not identical...
  12. ncbi request reprint Optical characterization of liposomes by right angle light scattering and turbidity measurement
    K Matsuzaki
    Graduate School of Biostudies and Graduate School of Pharmaceutical Sciences, Kyoto, Japan
    Biochim Biophys Acta 1467:219-26. 2000
    ....
  13. ncbi request reprint Why and how are peptide-lipid interactions utilized for self-defense? Magainins and tachyplesins as archetypes
    K Matsuzaki
    Graduate School of Biostudies, Kyoto University, Yoshida Shimoadachi Cho 46 29, Sakyo ku, Kyoto, Japan
    Biochim Biophys Acta 1462:1-10. 1999
    ....
  14. ncbi request reprint Interactions of an antimicrobial peptide, magainin 2, with lipopolysaccharide-containing liposomes as a model for outer membranes of gram-negative bacteria
    K Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
    FEBS Lett 449:221-4. 1999
    ..This system seems to be useful for investigating the molecular details of peptide-lipopolysaccharide interactions...
  15. ncbi request reprint Modulation of magainin 2-lipid bilayer interactions by peptide charge
    K Matsuzaki
    Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Japan
    Biochemistry 36:2104-11. 1997
    ..The charge distribution of wild-type magainin 2 was found to be so designed as to exhibit the maximal lytic activity by simultaneously achieving a strong binding and a moderate pore stability...
  16. ncbi request reprint Interactions of an antimicrobial peptide, magainin 2, with outer and inner membranes of Gram-negative bacteria
    K Matsuzaki
    Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Japan
    Biochim Biophys Acta 1327:119-30. 1997
    ..Substitution of Trp for Phe simultaneously increased the bactericidal activity and the hemolytic activity. A strategy to develop potent antimicrobial peptides was discussed on the basis of these results...
  17. pmc Pore formation and translocation of melittin
    K Matsuzaki
    Faculty of Pharmaceutical Sciences, Kyoto University, Japan
    Biophys J 73:831-8. 1997
    ..A novel model for the melittin pore formation was discussed in comparison with other pore-forming peptides...
  18. ncbi request reprint Relationship of membrane curvature to the formation of pores by magainin 2
    K Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
    Biochemistry 37:11856-63. 1998
    ..These results suggest that the peptide imposes positive curvature strain, facilitating the formation of a torus-type pore, and that the presence of negative curvature-inducing lipids inhibits pore formation...
  19. ncbi request reprint Mechanism of synergism between antimicrobial peptides magainin 2 and PGLa
    K Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
    Biochemistry 37:15144-53. 1998
    ..Therefore, the synergism is a consequence of the formation of a potent heterosupramolecular complex, which is characterized by fast pore formation and moderate pore stability...
  20. ncbi request reprint [Pivotal role of ganglioside in aggregation of amyloid beta-protein]
    Katsumi Matsuzaki
    Tanpakushitsu Kakusan Koso 49:2463-7. 2004
  21. ncbi request reprint Interactions of amyloid beta-peptide (1-40) with ganglioside-containing membranes
    K Matsuzaki
    Graduate School of Pharmaceutical Science, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 38:4137-42. 1999
    ..These results suggest that Abeta (1-40) imposes negative curvature strain on ganglioside-containing lipid bilayers, disturbing the structure and function of the membranes...
  22. ncbi request reprint An antimicrobial peptide, magainin 2, induced rapid flip-flop of phospholipids coupled with pore formation and peptide translocation
    K Matsuzaki
    Faculty of Pharmaceutical Sciences, Kyoto University, Japan
    Biochemistry 35:11361-8. 1996
    ..A simple theoretical calculation could explain the coupling of the flip-flop with the permeabilization...
  23. ncbi request reprint Bacteria-selective synergism between the antimicrobial peptides alpha-helical magainin 2 and cyclic beta-sheet tachyplesin I: toward cocktail therapy
    S Kobayashi
    Graduate Schools of Pharmaceutical Sciences and Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
    Biochemistry 40:14330-5. 2001
    ..These novel observations suggested the possibility of the development of cocktail therapeutic regimens using combinations of antimicrobial peptides...
  24. ncbi request reprint Transbilayer transport of ions and lipids coupled with mastoparan X translocation
    K Matsuzaki
    Faculty of Pharmaceutical Sciences, Kyoto University, Japan
    Biochemistry 35:8450-6. 1996
    ..A novel model of the mastoparan-lipid interactions was proposed to explain these observations...
  25. ncbi request reprint Cholesterol-dependent formation of GM1 ganglioside-bound amyloid beta-protein, an endogenous seed for Alzheimer amyloid
    A Kakio
    Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
    J Biol Chem 276:24985-90. 2001
    ..The results of the present study strongly suggested that increases in intramembrane cholesterol content, which are likely to occur during aging, appear to be a risk factor for amyloid fibril formation...
  26. doi request reprint Formation of toxic Abeta(1-40) fibrils on GM1 ganglioside-containing membranes mimicking lipid rafts: polymorphisms in Abeta(1-40) fibrils
    Takuma Okada
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Mol Biol 382:1066-74. 2008
    ..This study suggests that toxic Abeta(1-40) species formed on membranes are not soluble oligomers but amyloid fibrils and that Abeta(1-40) fibrils exhibit polymorphisms...
  27. ncbi request reprint Formation of amyloids by Abeta-(1-42) on NGF-differentiated PC12 cells: roles of gangliosides and cholesterol
    Masaki Wakabayashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, 606 8501, Japan
    J Mol Biol 371:924-33. 2007
    ..Our system using NGF-differentiated PC12 cells and Congo red is useful for screening inhibitors of the formation of amyloids by and cytotoxicity of Abeta...
  28. ncbi request reprint Formation of a membrane-active form of amyloid beta-protein in raft-like model membranes
    Atsuko Kakio
    Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Biophys Res Commun 303:514-8. 2003
    ..These results suggest that the lipid raft containing a ganglioside cluster serves as a conformational catalyst or a chaperon generating a membrane-active form of A beta with seeding ability...
  29. ncbi request reprint Interactions of amyloid beta-protein with various gangliosides in raft-like membranes: importance of GM1 ganglioside-bound form as an endogenous seed for Alzheimer amyloid
    Atsuko Kakio
    Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 41:7385-90. 2002
    ..GM1-Abeta exhibited the strongest seeding potential, especially under beta-sheet-forming conditions. This study suggested that lipid composition including gangliosides and cholesterol strictly controls amyloid formation...
  30. ncbi request reprint Formation of toxic fibrils of Alzheimer's amyloid beta-protein-(1-40) by monosialoganglioside GM1, a neuronal membrane component
    Takuma Okada
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    J Mol Biol 371:481-9. 2007
    ..Our study suggests an exacerbation mechanism of AD and an importance of polymorphisms in Abeta fibrils during the pathogenesis of the disease...
  31. doi request reprint Quality of life improvements in dialysis patients receiving darbepoetin alfa
    Shunichi Fukuhara
    Department of Epidemiology and Healthcare Research, Kyoto University Graduate School of Medicine and Public Health, Kyoto, Japan
    Ther Apher Dial 12:72-7. 2008
    ..Vitality increased significantly only in those patients with the greatest increases in Hb concentration and in those with higher final Hb concentrations...
  32. ncbi request reprint GM1 ganglioside-mediated accumulation of amyloid beta-protein on cell membranes
    Masaki Wakabayashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Biophys Res Commun 328:1019-23. 2005
    ..Cholesterol depletion significantly reduced Abeta accumulation. These observations corroborate the GM1-mediated Abeta accumulation model...
  33. ncbi request reprint Inhibition of the formation of amyloid beta-protein fibrils using biocompatible nanogels as artificial chaperones
    Keisuke Ikeda
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, 606 8501, Japan
    FEBS Lett 580:6587-95. 2006
    ..In addition, CHPNH(2) nanogels protected PC12 cells from Abeta toxicity...
  34. doi request reprint Ganglioside-induced amyloid formation by human islet amyloid polypeptide in lipid rafts
    Masaki Wakabayashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
    FEBS Lett 583:2854-8. 2009
    ..The depletion of gangliosides or cholesterol significantly reduced the amount of amyloid deposited. These results clearly showed that the formation of amyloid fibrils was mediated by gangliosides in lipid rafts...
  35. ncbi request reprint Membrane translocation mechanism of the antimicrobial peptide buforin 2
    Satoe Kobayashi
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 43:15610-6. 2004
    ..The presence of Pro(11) distorts the helix, concentrating basic amino acid residues in a limited amphipathic region, thus destabilizing the pore by enhanced electrostatic repulsion, enabling efficient translocation...
  36. ncbi request reprint "Click peptide": pH-triggered in situ production and aggregation of monomer Abeta1-42
    Atsuhiko Taniguchi
    Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina ku, Kyoto, Japan
    Chembiochem 10:710-5. 2009
    ..This click peptide strategy should provide a reliable experimental system to investigate the pathological role of Abeta1-42 in Alzheimer's disease...
  37. doi request reprint A disulfide-linked amyloid-beta peptide dimer forms a protofibril-like oligomer through a distinct pathway from amyloid fibril formation
    Takahiro Yamaguchi
    Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 49:7100-7. 2010
    ..The results emphasize the importance of the conformational flexibility of the Abeta molecule and a balance in the association and dissociation rate for the formation of rigid amyloid fibrils...
  38. ncbi request reprint Abeta polymerization through interaction with membrane gangliosides
    Katsumi Matsuzaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1801:868-77. 2010
    ..In this review, we focus on the recent progress of GAbeta studies and highlight the possibility that ganglioside binding is the initial and common step in the development of a part of human misfolding-type amyloidoses, including AD...
  39. ncbi request reprint Topological stability and self-association of a completely hydrophobic model transmembrane helix in lipid bilayers
    Yoshiaki Yano
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 41:3073-80. 2002
    ..Electron spin resonance spectra of 1-palmitoyl-2-stearoyl-(14-doxyl)-sn-glycero-3-phosphocholine demonstrated the presence of a motionally restricted component at lower temperatures...
  40. ncbi request reprint Position-dependent hydrophobicity of the antimicrobial magainin peptide affects the mode of peptide-lipid interactions and selective toxicity
    Tomoya Tachi
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 41:10723-31. 2002
    ..These observations demonstrate that the hydrophobicity of the peptide also controls the mode of action and is dependent on the position of the hydrophobic amino acids in the peptide sequence...
  41. ncbi request reprint Membrane insertion and dissociation processes of a model transmembrane helix
    Yoshiaki Yano
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 41:12407-13. 2002
    ..On the basis of these observations, a model for the helix insertion/dissociation processes was discussed...
  42. doi request reprint Driving force of binding of amyloid beta-protein to lipid bilayers
    Keisuke Ikeda
    Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Biophys Res Commun 370:525-9. 2008
    ..These results suggested that hydrogen-bonding as well as hydrophobic interactions with sugar groups of glycolipids drive the membrane binding of Abeta-(1-40)...
  43. doi request reprint Controlled production of amyloid beta peptide from a photo-triggered, water-soluble precursor "click peptide"
    Atsuhiko Taniguchi
    Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, Kyoto Pharmaceutical University, Kyoto, Japan
    Chembiochem 9:3055-65. 2008
    ....
  44. ncbi request reprint Action mechanism of tachyplesin I and effects of PEGylation
    Yuichi Imura
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1768:1160-9. 2007
    ..Furthermore, a reduction in DNA binding by PEGylation may also contribute to the weak activity of the PEGylated peptide...
  45. ncbi request reprint High-performance frontal analysis of the binding of thyroxine enantiomers to human serum albumin
    Tomoko Kimura
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, 606 8501, Japan
    Pharm Res 22:667-75. 2005
    ..The aim of this study was to characterize the binding property between thyroxine and human serum albumin (HSA) qualitatively and enantioselectively using high-performance frontal analysis (HPFA)...
  46. ncbi request reprint Measurement of thermodynamic parameters for hydrophobic mismatch 1: self-association of a transmembrane helix
    Yoshiaki Yano
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 45:3370-8. 2006
    ..The new concept of dipole-dipole interaction as a basic driving force of helix dimerization will become a basis for understanding the structural and functional modifications in response to hydrophobic mismatch...
  47. ncbi request reprint Translocation of analogues of the antimicrobial peptides magainin and buforin across human cell membranes
    Kenta Takeshima
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Japan
    J Biol Chem 278:1310-5. 2003
    ..However, the mechanism appeared to be different from that of the parent peptide...
  48. ncbi request reprint Biantennary glycans as well as genetic variants of alpha1-acid glycoprotein control the enantioselectivity and binding affinity of oxybutynin
    Tomoko Kimura
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Pharm Res 23:1038-42. 2006
    ..The purpose of this study was to investigate the role of biantennary branching glycans of alpha1-acid glycoprotein (AGP) and its genetic variants in the enantioselective binding of oxybutynin (OXY)...
  49. ncbi request reprint Measurement of thermodynamic parameters for hydrophobic mismatch 2: intermembrane transfer of a transmembrane helix
    Yoshiaki Yano
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 45:3379-85. 2006
    ....
  50. ncbi request reprint Action mechanism of PEGylated magainin 2 analogue peptide
    Yuichi Imura
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochim Biophys Acta 1768:2578-85. 2007
    ..At the same time, the antimicrobial activity was weakened only by 4 fold. The effects of PEGylation on the activity of magainin were compared with those for tachyplesin...
  51. ncbi request reprint Simultaneous determination of unbound thyroid hormones in human plasma using high performance frontal analysis with electrochemical detection
    Tomoko Kimura
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Pharm Biomed Anal 38:204-9. 2005
    ..The CV% values of intra-day and inter-day assays (n=15) were less than 14.9% for T4, 14.5% for T3 and 13.2% for rT3. The present system was also applied to a competitive binding study of these thyroid hormones in human plasma...
  52. doi request reprint Design of a soluble transmembrane helix for measurements of water-membrane partitioning
    Yoshiaki Yano
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Phys Chem B 114:1925-31. 2010
    ..6 kJ mol(-1). Thus, the transmembrane helix was found to be a useful template for thermodynamic measurements of the partitioning of amino acids from water to the hydrophobic core of membranes...
  53. pmc Magainin 2 in action: distinct modes of membrane permeabilization in living bacterial and mammalian cells
    Yuichi Imura
    Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biophys J 95:5757-65. 2008
    ..Thus, the distinct membrane-permeabilizing processes of magainin 2 in bacterial and mammalian cells were, to the best of our knowledge, visualized and characterized in detail for the first time...
  54. doi request reprint Intracellular third loop-C-terminal tail interaction in prostaglandin EP3beta receptor
    Yoshiaki Yano
    Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan
    Biochem Biophys Res Commun 371:846-9. 2008
    ..These results demonstrate for the first time specific interaction between the intracellular third loop and the C-terminus. A model is proposed for the activation of the receptor...
  55. ncbi request reprint Interaction of a magainin-PGLa hybrid peptide with membranes: insight into the mechanism of synergism
    Minoru Nishida
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 46:14284-90. 2007
    ..Thus, the separate production of mutually recognizing peptides without cross-linking appears to be a good way to increase selective toxicity...
  56. ncbi request reprint Environment- and mutation-dependent aggregation behavior of Alzheimer amyloid beta-protein
    Naoki Yamamoto
    Department of Dementia Research, National Institute for Longevity Sciences, Obu, Japan
    J Neurochem 90:62-9. 2004
    ..These results suggest that there are favorable local environments for fibrillogenesis of amyloid beta-protein...
  57. ncbi request reprint Cholesterol-dependent aggregation of amyloid beta-protein
    Katsuhiko Yanagisawa
    Department of Dementia Research, National Institute for Longevity Sciences, 36 3 Gengo, Morioka, Obu 474 8522, Japan
    Ann N Y Acad Sci 977:384-6. 2002
    ..The results of our current studies may provide a new insight into the molecular mechanism underlying the cholesterol-dependent development of AD...
  58. ncbi request reprint Cinnamycin (Ro 09-0198) promotes cell binding and toxicity by inducing transbilayer lipid movement
    Asami Makino
    Supra Biomolecular System Research Group, RIKEN Institute of Physical and Chemical Research, Frontier Research System, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    J Biol Chem 278:3204-9. 2003
    ..These results suggest that cinnamycin promotes its own binding to the cell and causes toxicity by inducing transbilayer lipid movement...
  59. ncbi request reprint 'Click peptide': a novel 'O-acyl isopeptide method' for peptide synthesis and chemical biology-oriented synthesis of amyloid beta peptide analogues
    Youhei Sohma
    Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina ku, Kyoto 607 8412
    J Pept Sci 12:823-8. 2006
    ....
  60. ncbi request reprint Amyloid beta-protein (Abeta)1-40 protects neurons from damage induced by Abeta1-42 in culture and in rat brain
    Kun Zou
    Department of Dementia Research, National Institute for Longevity Sciences, Aichi, Japan
    J Neurochem 87:609-19. 2003
    ..Our data suggest a mechanism by which elevated Abeta1-42/Abeta1-40 ratio accelerates the development of Alzheimer's disease (AD) in familial AD...
  61. ncbi request reprint A seed for Alzheimer amyloid in the brain
    Hideki Hayashi
    Department of Dementia Research, National Institute for Longevity Sciences, Obu 474 8522, Japan
    J Neurosci 24:4894-902. 2004
    ..These results imply a mechanism underlying the onset of AD and suggest that an endogenous seed can be a target of therapeutic strategy...
  62. ncbi request reprint Specific interactions of the antimicrobial peptide cyclic beta-sheet tachyplesin I with lipopolysaccharides
    Yutaka Hirakura
    Advanced Research Center for Human Sciences, Waseda University, Nishi Tokyo, Tokyo 202 0021, Japan
    Biochim Biophys Acta 1562:32-6. 2002
    ..The recognition site was the lipid A moiety and the cyclic structure was crucial to this specific binding. The cyclic structure also endowed the peptide with very rapid outer membrane (OM) permeabilization...
  63. ncbi request reprint Cross-seeding of wild-type and hereditary variant-type amyloid beta-proteins in the presence of gangliosides
    Naoki Yamamoto
    Department of Alzheimer s Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Morioka, Obu, Japan
    J Neurochem 95:1167-76. 2005
    ..This study suggests that cross-seeding can occur between wild and hereditary variant-type amyloid beta-proteins despite differences in their amino acid sequences...