Gen Kondoh

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization
    Gen Kondoh
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, 2 2 Yamadaoka, Osaka 565 0871, Japan
    Nat Med 11:160-6. 2005
  2. doi request reprint Testicular Angiotensin-converting enzyme with different glycan modification: characterization on glycosylphosphatidylinositol-anchored protein releasing and dipeptidase activities
    Gen Kondoh
    Laboratory of Animal Experiments for Regeneration, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    J Biochem 145:115-21. 2009
  3. ncbi request reprint Dipeptidase-inactivated tACE action in vivo: selective inhibition of sperm-zona pellucida binding in the mouse
    Eishi Deguchi
    Institute for Frontier Medical Sciences, Kyoto University, Japan
    Biol Reprod 77:794-802. 2007
  4. pmc Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth
    Kojiro Yae
    Department of Social and Environmental Medicine H3, Osaka University, Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 26:6185-96. 2006
  5. ncbi request reprint Genome-wide phenotype analysis in ES cells by regulated disruption of Bloom's syndrome gene
    Kosuke Yusa
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Nature 429:896-9. 2004
  6. ncbi request reprint Ahnak/Desmoyokin is dispensable for proliferation, differentiation, and maintenance of integrity in mouse epidermis
    Michiyoshi Kouno
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan
    J Invest Dermatol 123:700-7. 2004
  7. ncbi request reprint Enhanced insulin sensitivity, energy expenditure and thermogenesis in adipose-specific Pten suppression in mice
    Nobuyasu Komazawa
    Department of Social and Environmental Medicine, Graduate School of Frontier Bioscience, Osaka University, 2 2 Yamadaoka, Suita, Osaka, Japan
    Nat Med 10:1208-15. 2004
  8. ncbi request reprint Tumorigenesis facilitated by Pten deficiency in the skin: evidence of p53-Pten complex formation on the initiation phase
    Nobuyasu Komazawa
    Department of Social and Environmental Medicine, Osaka University, Suita, 565 0871, Japan
    Cancer Sci 95:639-43. 2004
  9. pmc Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-specific Arnt-deficient mice affects skin barrier function
    Satoshi Takagi
    Department of Social and Environmental Medicine H3, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    J Clin Invest 112:1372-82. 2003
  10. pmc Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa
    Yoshitaka Fujihara
    Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 110:8111-6. 2013

Collaborators

Detail Information

Publications23

  1. ncbi request reprint Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization
    Gen Kondoh
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, 2 2 Yamadaoka, Osaka 565 0871, Japan
    Nat Med 11:160-6. 2005
    ..Moreover, peptidase-inactivated E414D mutant ACE and also PI-PLC rescued the egg-binding deficiency of Ace knockout sperms, implying that ACE plays a crucial role in fertilization through this activity...
  2. doi request reprint Testicular Angiotensin-converting enzyme with different glycan modification: characterization on glycosylphosphatidylinositol-anchored protein releasing and dipeptidase activities
    Gen Kondoh
    Laboratory of Animal Experiments for Regeneration, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    J Biochem 145:115-21. 2009
    ....
  3. ncbi request reprint Dipeptidase-inactivated tACE action in vivo: selective inhibition of sperm-zona pellucida binding in the mouse
    Eishi Deguchi
    Institute for Frontier Medical Sciences, Kyoto University, Japan
    Biol Reprod 77:794-802. 2007
    ..Our results indicate that the dipeptidase-inactivated tACE acts in vivo, suggesting that tACE contributes to fertilization as a dipeptidase at least in the epididymis...
  4. pmc Sleeping beauty transposon-based phenotypic analysis of mice: lack of Arpc3 results in defective trophoblast outgrowth
    Kojiro Yae
    Department of Social and Environmental Medicine H3, Osaka University, Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 26:6185-96. 2006
    ..These data indicate the importance of Arpc3 in the Arp2/3 complex for trophoblast outgrowth and suggest that Arpc3 may be indispensable for implantation...
  5. ncbi request reprint Genome-wide phenotype analysis in ES cells by regulated disruption of Bloom's syndrome gene
    Kosuke Yusa
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Suita, Osaka 565 0871, Japan
    Nature 429:896-9. 2004
    ..Our results indicate that phenotype-based genetic screening with Blm(tet) is very efficient and raises possibilities for identifying gene functions in ES cells...
  6. ncbi request reprint Ahnak/Desmoyokin is dispensable for proliferation, differentiation, and maintenance of integrity in mouse epidermis
    Michiyoshi Kouno
    Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan
    J Invest Dermatol 123:700-7. 2004
    ..These results lead us to conclude that Ahnak/Desmoyokin deficiency has only a minimal effect on epidermal cell adhesion, tumorigenesis, cell proliferation and differentiation, and overall mouse development...
  7. ncbi request reprint Enhanced insulin sensitivity, energy expenditure and thermogenesis in adipose-specific Pten suppression in mice
    Nobuyasu Komazawa
    Department of Social and Environmental Medicine, Graduate School of Frontier Bioscience, Osaka University, 2 2 Yamadaoka, Suita, Osaka, Japan
    Nat Med 10:1208-15. 2004
    ..Our results suggest that altered expression of adipose Pten could regulate insulin sensitivity and energy expenditure. Suppression of adipose Pten may become a beneficial strategy to treat type 2 diabetes and obesity...
  8. ncbi request reprint Tumorigenesis facilitated by Pten deficiency in the skin: evidence of p53-Pten complex formation on the initiation phase
    Nobuyasu Komazawa
    Department of Social and Environmental Medicine, Osaka University, Suita, 565 0871, Japan
    Cancer Sci 95:639-43. 2004
    ..These observations suggest that Pten is involved in rapid recruitment of p53 in the tumor initiation phase...
  9. pmc Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-specific Arnt-deficient mice affects skin barrier function
    Satoshi Takagi
    Department of Social and Environmental Medicine H3, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    J Clin Invest 112:1372-82. 2003
    ..These results indicate that proper ceramide compositions through 4-desaturation regulated by ARNT are crucial for maintaining the epidermal barrier function...
  10. pmc Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa
    Yoshitaka Fujihara
    Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 110:8111-6. 2013
    ..The finding of TEX101 as a unique specific substrate for ACE may provide a potential target for the production of an awaited contraceptive medicine for men...
  11. ncbi request reprint Lipocalin 2 binds to membrane phosphatidylethanolamine to induce lipid raft movement in a PKA-dependent manner and modulates sperm maturation
    Hitomi Watanabe
    Laboratory of Animal Experiments for Regeneration, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Development 141:2157-64. 2014
    ..These observations imply that mammals possess a mode for sperm maturation in addition to the albumin-mediated pathway...
  12. pmc An in vitro ES cell-based clock recapitulation assay model identifies CK2α as an endogenous clock regulator
    Yasuhiro Umemura
    Department of Physiology and Systems Bioscience, Kyoto Prefectural University of Medicine, Kyoto, Japan
    PLoS ONE 8:e67241. 2013
    ..This assay was successfully applied for the phenotype analysis of homozygous mutant ES cells, demonstrating that an ES cell-based in vitro assay is available for circadian genetic screening. ..
  13. doi request reprint Mammalian carboxylesterase (CES) releases GPI-anchored proteins from the cell surface upon lipid raft fluidization
    Kaoru Orihashi
    Laboratory of Animal Experiments for Regeneration, Institute for Frontier Medical Sciences, Kyoto University, 53 Syogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
    Biol Chem 393:169-76. 2012
    ..The released product contained a GPI component, indicating that GPI-AP was released by cleavage in GPI. These results revealed for the first time that CES recognizes and catalyzes macromolecule GPI-AP as well as small molecules...
  14. ncbi request reprint Development of glycosylphosphatidylinositol-anchored enhanced green fluorescent protein. One-step visualization of GPI fate in global tissues and ubiquitous cell surface marking
    Gen Kondoh
    Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine, Osaka, Japan
    Methods Mol Biol 183:215-24. 2002
  15. doi request reprint Mouse sperm undergo GPI-anchored protein release associated with lipid raft reorganization and acrosome reaction to acquire fertility
    Hitomi Watanabe
    Laboratory of Animal Experiments for Regeneration, Institute for Frontier Medical Sciences, Kyoto University and CREST Program, Japan Science and Technology Society, 53 Syogoin Kawahara cho, Kyoto 606 8507, Japan
    J Cell Sci 124:2573-81. 2011
    ..These results suggest that sperm undergoing GPI-anchored protein release associated with reorganization of lipid rafts and the acrosome reaction acquire fertilization potential...
  16. pmc Characterization of Sleeping Beauty transposition and its application to genetic screening in mice
    Kyoji Horie
    Collaborative Research Center for Advanced Science and Technology, Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 23:9189-207. 2003
    ..Our results indicate that the SB transposon system has unique features that complement other mutagenesis approaches...
  17. doi request reprint Fezf1 is required for penetration of the basal lamina by olfactory axons to promote olfactory development
    Yasuhito Watanabe
    Osaka Bioscience Institute, Suita, Osaka 565 0874, Japan
    J Comp Neurol 515:565-84. 2009
    ..These data indicate that Fezf1 is required for the penetration of olfactory axons through the CNS basal lamina before they innervate the OB...
  18. doi request reprint The essential functions of adipo-osteogenic progenitors as the hematopoietic stem and progenitor cell niche
    Yoshiki Omatsu
    Department of Immunobiology and Hematology, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto, Japan
    Immunity 33:387-99. 2010
    ..Thus, the niche composed of adipo-osteogenic progenitors is required for proliferation of HSCs and lymphoid and erythroid progenitors, as well as maintenance of HSCs in an undifferentiated state...
  19. pmc Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis
    Takashi Tanaka
    Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 108:10579-84. 2011
    ....
  20. pmc p38alpha mitogen-activated protein kinase plays a critical role in cardiomyocyte survival but not in cardiac hypertrophic growth in response to pressure overload
    Kazuhiko Nishida
    Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Cell Biol 24:10611-20. 2004
    ..These results demonstrate that p38alpha plays a critical role in the cardiomyocyte survival pathway in response to pressure overload, while cardiac hypertrophic growth is unaffected despite its dramatic down-regulation...
  21. ncbi request reprint Rapid compensation for glycosylphosphatidylinositol anchor deficient keratinocytes after birth: visualization of glycosylphosphatidylinositol-anchored proteins in situ
    Xing Hua Gao
    Department of Social and Environmental Medicine H3 and Department of Dermatology, Graduate School of Medicine, Osaka University, Japan
    J Invest Dermatol 118:998-1002. 2002
    ..These observations suggest that the GPI-anchoring is associated with the barrier function as well as with organized differentiation of the epidermis after birth...
  22. doi request reprint The molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation
    Yusaku Masago
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, 606 8507, Japan
    J Cell Sci 125:1118-28. 2012
    ..These results demonstrate that Hsp47 is indispensable for well-organized cartilage and normal endochondral bone formation...
  23. doi request reprint The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline
    Masanobu Shoji
    Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Dev Cell 17:775-87. 2009
    ....