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Genomes and Genes | Shigeyuki KawaiSummaryAffiliation: Kyoto University Country: Japan Publications
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Publications
Mitochondrial genomic dysfunction causes dephosphorylation of Sch9 in the yeast Saccharomyces cerevisiaeShigeyuki Kawai
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
Eukaryot Cell 10:1367-9. 2011....
Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H)Shigeyuki Kawai
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
Biosci Biotechnol Biochem 72:919-30. 2008..Research on NAD kinase also led to the discovery of archaeal NADP phosphatase. In this review, we summarize the physiological functions, applications, and structure of NAD kinase, and the way we discovered archaeal NADP phosphatase...- [Function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H)]Shigeyuki Kawai
Tanpakushitsu Kakusan Koso 52:243-8. 2007 - Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiaeFeng Shi
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
FEBS J 272:3337-49. 2005..cerevisiae did not. The complementation was putatively attributed to an ability of Leu2p of C. glabrata to use NADP as a coenzyme and to supply NADPH... - Structural determinants of discrimination of NAD+ from NADH in yeast mitochondrial NADH kinase Pos5Takuya Ando
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
J Biol Chem 286:29984-92. 2011..Thus, Arg-293 of Pos5 is a major determinant of NADH selectivity. Moreover, Ser-272 appears to assist Arg-293 in achieving the appropriate conformation... - Hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase familyShigeyuki Kawai
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
J Biosci Bioeng 99:320-30. 2005.... - Cytosolic NADP phosphatases I and II from Arthrobacter sp. strain KM: implication in regulation of NAD+/NADP+ balanceShigeyuki Kawai
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan
J Basic Microbiol 44:185-96. 2004..Comparison of the features of NADPases I and II with those of other acid phosphatases possessing NADPase activity suggested that NADPases I and II are novel enzymes participating in regulation of the NAD+/NADP+ balance in the cytosol... - Two sources of mitochondrial NADPH in the yeast Saccharomyces cerevisiaeHikaru Miyagi
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
J Biol Chem 284:7553-60. 2009.... - MJ0917 in archaeon Methanococcus jannaschii is a novel NADP phosphatase/NAD kinaseShigeyuki Kawai
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
J Biol Chem 280:39200-7. 2005..The evolutionary process during which MJ0917 arose is also discussed... - NADP(H) phosphatase activities of archaeal inositol monophosphatase and eubacterial 3'-phosphoadenosine 5'-phosphate phosphataseChikako Fukuda
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
Appl Environ Microbiol 73:5447-52. 2007..However, the kinetic values of CysQ and the known phenotype of the cysQ mutant indicated that CysQ functions physiologically as 3'-phosphoadenosine 5'-phosphate phosphatase rather than as NADP(H) phosphatase... - Transformation of Saccharomyces cerevisiae and other fungi: methods and possible underlying mechanismShigeyuki Kawai
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Bioeng Bugs 1:395-403. 2010..Both lithium acetate and heat shock, which enhance the transformation efficiency of intact cells but not that of spheroplasts, probably help DNA to pass through the cell wall... 
NADPH regulates human NAD kinase, a NADP⁺-biosynthetic enzymeKazuto Ohashi
Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
Mol Cell Biochem 355:57-64. 2011..Furthermore, these attributes may provide a clue to design a novel drug against Mycobacterium tuberculosis in which this bacterial NADK is potently inhibited by NADP(+)...- NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complexShigetarou Mori
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
Biochem Biophys Res Commun 327:500-8. 2005..The two residues and the residues involved in NAD-binding were conserved. However, residues corresponding to the Ppnk-flexible loop were not conserved, making us to speculate that the Ppnk-flexible loop may be Ppnk-specific... - Crystal structure of bacterial inorganic polyphosphate/ATP-glucomannokinase. Insights into kinase evolutionTakako Mukai
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan
J Biol Chem 279:50591-600. 2004.... - Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteriaYuji Aso
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan
Nat Biotechnol 24:188-9. 2006..In both cases the bioremediation capability of the organisms was substantially increased pointing at the potential of this approach as a general strategy to improve bacterial degradation of hazardous compounds in the environment... - Molecular conversion of NAD kinase to NADH kinase through single amino acid residue substitutionShigetarou Mori
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
J Biol Chem 280:24104-12. 2005..The significance of the conservation of the residue at the position in 207 NAD kinase homologues is also discussed... - Overexpression, purification, and characterization of ATP-NAD kinase of Sphingomonas sp. A1Akihito Ochiai
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, 611-0011, Japan
Protein Expr Purif 36:124-30. 2004..Taken together with the report that the NAD kinase of Bacillus subtilis is activated by quinolinic acid [J. Bacteriol. 185 (2003) 4844], it is indicated that the regulatory patterns of NAD kinases differ even among bacterial NAD kinases... - Bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applicationsWataru Hashimoto
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
Bioeng Bugs 1:97-109. 2010..Furthermore, strain A1 cells transformed with Zymomonas mobilis genes for pyruvate decarboxylase and alcohol dehydrogenase II produce considerable amounts of biofuel ethanol from alginate when grown statically... - The role of cell wall revealed by the visualization of Saccharomyces cerevisiae transformationTuan Anh Pham
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611 0011, Japan
Curr Microbiol 62:956-61. 2011.... - Primary structure of inorganic polyphosphate/ATP-NAD kinase from Micrococcus flavus, and occurrence of substrate inorganic polyphosphate for the enzymeShigeyuki Kawai
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan
Biosci Biotechnol Biochem 67:1751-60. 2003..flavus, suggesting that the ability of the enzyme to use inorganic polyphosphate is of physiological significance and is not an evolutionary trait alone... - Superchannel of bacteria: biological significance and new horizonsKousaku Murata
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
Biosci Biotechnol Biochem 72:265-77. 2008.... - Crystallization and preliminary X-ray analysis of inorganic polyphosphate/ATP-glucomannokinase from Arthrobacter sp. strain KMTakako Mukai
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan
Acta Crystallogr D Biol Crystallogr 59:1662-4. 2003..2, b = 83.7, c = 103.8 A. Assuming two molecules per asymmetric unit, V(sol) is 0.49. X-ray diffraction data to 2.83 A have been collected from a single crystal... - Transcriptional and metabolic response in yeast Saccharomyces cerevisiae cells during polyethylene glycol-dependent transformationShigeyuki Kawai
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
J Basic Microbiol 49:73-81. 2009.... - Molecular insights on DNA delivery into Saccharomyces cerevisiaeShigeyuki Kawai
Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan
Biochem Biophys Res Commun 317:100-7. 2004..We also propose that DNA is taken up via an endocytosis-like event, being at least partially different from well-known endocytosis... - Visualization of the synergistic effect of lithium acetate and single-stranded carrier DNA on Saccharomyces cerevisiae transformationTuan Anh Pham
Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611 0011, Japan
Curr Genet 57:233-9. 2011..Together, the two compounds synergistically enhance the transformation efficiency and frequency... - Identification and characterization of a human mitochondrial NAD kinaseKazuto Ohashi
Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
Nat Commun 3:1248. 2012..These observations provide insights into eukaryotic NADP(+) biosynthesis, which has pivotal roles in cells and organelles... - Significance of sulfiredoxin/peroxiredoxin and mitochondrial respiratory chain in response to and protection from 100% O(2) in Saccharomyces cerevisiaeHiroki Fujiwara
Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
Mitochondrion 13:52-8. 2013.... - Characterization and molecular cloning of a novel enzyme, inorganic polyphosphate/ATP-glucomannokinase, of Arthrobacter sp. strain KMTakako Mukai
Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan
Appl Environ Microbiol 69:3849-57. 2003..Alignment of these homologous proteins revealed seven conserved regions. The mannose and poly(P) binding sites of poly(P)/ATP-glucomannokinase are discussed...