Shigeyuki Kawai

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. doi request reprint Bacterial pyruvate production from alginate, a promising carbon source from marine brown macroalgae
    Shigeyuki Kawai
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan Electronic address
    J Biosci Bioeng 117:269-74. 2014
  2. pmc Mitochondrial genomic dysfunction causes dephosphorylation of Sch9 in the yeast Saccharomyces cerevisiae
    Shigeyuki Kawai
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Eukaryot Cell 10:1367-9. 2011
  3. ncbi request reprint Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H)
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biosci Biotechnol Biochem 72:919-30. 2008
  4. ncbi request reprint [Function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H)]
    Shigeyuki Kawai
    Tanpakushitsu Kakusan Koso 52:243-8. 2007
  5. ncbi request reprint Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae
    Feng Shi
    Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    FEBS J 272:3337-49. 2005
  6. pmc Structural determinants of discrimination of NAD+ from NADH in yeast mitochondrial NADH kinase Pos5
    Takuya Ando
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    J Biol Chem 286:29984-92. 2011
  7. ncbi request reprint Hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase family
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biosci Bioeng 99:320-30. 2005
  8. ncbi request reprint Cytosolic NADP phosphatases I and II from Arthrobacter sp. strain KM: implication in regulation of NAD+/NADP+ balance
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Basic Microbiol 44:185-96. 2004
  9. pmc Two sources of mitochondrial NADPH in the yeast Saccharomyces cerevisiae
    Hikaru Miyagi
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 284:7553-60. 2009
  10. ncbi request reprint MJ0917 in archaeon Methanococcus jannaschii is a novel NADP phosphatase/NAD kinase
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 280:39200-7. 2005

Collaborators

Detail Information

Publications33

  1. doi request reprint Bacterial pyruvate production from alginate, a promising carbon source from marine brown macroalgae
    Shigeyuki Kawai
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan Electronic address
    J Biosci Bioeng 117:269-74. 2014
    ..Our study is the first report to demonstrate the production of other valuable compounds than ethanol from alginate, a promising marine macroalgae carbon source. ..
  2. pmc Mitochondrial genomic dysfunction causes dephosphorylation of Sch9 in the yeast Saccharomyces cerevisiae
    Shigeyuki Kawai
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Eukaryot Cell 10:1367-9. 2011
    ....
  3. ncbi request reprint Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H)
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biosci Biotechnol Biochem 72:919-30. 2008
    ..Research on NAD kinase also led to the discovery of archaeal NADP phosphatase. In this review, we summarize the physiological functions, applications, and structure of NAD kinase, and the way we discovered archaeal NADP phosphatase...
  4. ncbi request reprint [Function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H)]
    Shigeyuki Kawai
    Tanpakushitsu Kakusan Koso 52:243-8. 2007
  5. ncbi request reprint Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae
    Feng Shi
    Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    FEBS J 272:3337-49. 2005
    ..cerevisiae did not. The complementation was putatively attributed to an ability of Leu2p of C. glabrata to use NADP as a coenzyme and to supply NADPH...
  6. pmc Structural determinants of discrimination of NAD+ from NADH in yeast mitochondrial NADH kinase Pos5
    Takuya Ando
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    J Biol Chem 286:29984-92. 2011
    ..Thus, Arg-293 of Pos5 is a major determinant of NADH selectivity. Moreover, Ser-272 appears to assist Arg-293 in achieving the appropriate conformation...
  7. ncbi request reprint Hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase family
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biosci Bioeng 99:320-30. 2005
    ....
  8. ncbi request reprint Cytosolic NADP phosphatases I and II from Arthrobacter sp. strain KM: implication in regulation of NAD+/NADP+ balance
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Basic Microbiol 44:185-96. 2004
    ..Comparison of the features of NADPases I and II with those of other acid phosphatases possessing NADPase activity suggested that NADPases I and II are novel enzymes participating in regulation of the NAD+/NADP+ balance in the cytosol...
  9. pmc Two sources of mitochondrial NADPH in the yeast Saccharomyces cerevisiae
    Hikaru Miyagi
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 284:7553-60. 2009
    ....
  10. ncbi request reprint MJ0917 in archaeon Methanococcus jannaschii is a novel NADP phosphatase/NAD kinase
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 280:39200-7. 2005
    ..The evolutionary process during which MJ0917 arose is also discussed...
  11. doi request reprint NADPH regulates human NAD kinase, a NADP⁺-biosynthetic enzyme
    Kazuto Ohashi
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Mol Cell Biochem 355:57-64. 2011
    ..Furthermore, these attributes may provide a clue to design a novel drug against Mycobacterium tuberculosis in which this bacterial NADK is potently inhibited by NADP(+)...
  12. pmc NADP(H) phosphatase activities of archaeal inositol monophosphatase and eubacterial 3'-phosphoadenosine 5'-phosphate phosphatase
    Chikako Fukuda
    Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Environ Microbiol 73:5447-52. 2007
    ..However, the kinetic values of CysQ and the known phenotype of the cysQ mutant indicated that CysQ functions physiologically as 3'-phosphoadenosine 5'-phosphate phosphatase rather than as NADP(H) phosphatase...
  13. pmc Identification and characterization of a human mitochondrial NAD kinase
    Kazuto Ohashi
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Nat Commun 3:1248. 2012
    ..These observations provide insights into eukaryotic NADP(+) biosynthesis, which has pivotal roles in cells and organelles...
  14. pmc Transformation of Saccharomyces cerevisiae and other fungi: methods and possible underlying mechanism
    Shigeyuki Kawai
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Bioeng Bugs 1:395-403. 2010
    ..Both lithium acetate and heat shock, which enhance the transformation efficiency of intact cells but not that of spheroplasts, probably help DNA to pass through the cell wall...
  15. ncbi request reprint NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex
    Shigetarou Mori
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 327:500-8. 2005
    ..The two residues and the residues involved in NAD-binding were conserved. However, residues corresponding to the Ppnk-flexible loop were not conserved, making us to speculate that the Ppnk-flexible loop may be Ppnk-specific...
  16. ncbi request reprint Crystal structure of bacterial inorganic polyphosphate/ATP-glucomannokinase. Insights into kinase evolution
    Takako Mukai
    Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 279:50591-600. 2004
    ....
  17. ncbi request reprint Overexpression, purification, and characterization of ATP-NAD kinase of Sphingomonas sp. A1
    Akihito Ochiai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, 611 0011, Japan
    Protein Expr Purif 36:124-30. 2004
    ..Taken together with the report that the NAD kinase of Bacillus subtilis is activated by quinolinic acid [J. Bacteriol. 185 (2003) 4844], it is indicated that the regulatory patterns of NAD kinases differ even among bacterial NAD kinases...
  18. ncbi request reprint Molecular conversion of NAD kinase to NADH kinase through single amino acid residue substitution
    Shigetarou Mori
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 280:24104-12. 2005
    ..The significance of the conservation of the residue at the position in 207 NAD kinase homologues is also discussed...
  19. pmc Bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications
    Wataru Hashimoto
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    Bioeng Bugs 1:97-109. 2010
    ..Furthermore, strain A1 cells transformed with Zymomonas mobilis genes for pyruvate decarboxylase and alcohol dehydrogenase II produce considerable amounts of biofuel ethanol from alginate when grown statically...
  20. doi request reprint The role of cell wall revealed by the visualization of Saccharomyces cerevisiae transformation
    Tuan Anh Pham
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611 0011, Japan
    Curr Microbiol 62:956-61. 2011
    ....
  21. ncbi request reprint Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria
    Yuji Aso
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Nat Biotechnol 24:188-9. 2006
    ..In both cases the bioremediation capability of the organisms was substantially increased pointing at the potential of this approach as a general strategy to improve bacterial degradation of hazardous compounds in the environment...
  22. ncbi request reprint Primary structure of inorganic polyphosphate/ATP-NAD kinase from Micrococcus flavus, and occurrence of substrate inorganic polyphosphate for the enzyme
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biosci Biotechnol Biochem 67:1751-60. 2003
    ..flavus, suggesting that the ability of the enzyme to use inorganic polyphosphate is of physiological significance and is not an evolutionary trait alone...
  23. ncbi request reprint Superchannel of bacteria: biological significance and new horizons
    Kousaku Murata
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biosci Biotechnol Biochem 72:265-77. 2008
    ....
  24. pmc Secretion of quinolinic acid, an intermediate in the kynurenine pathway, for utilization in NAD+ biosynthesis in the yeast Saccharomyces cerevisiae
    Kazuto Ohashi
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    Eukaryot Cell 12:648-53. 2013
    ..Our data thus shed new light on the significance of quinolinic acid, which had previously been recognized only as an intermediate in the kynurenine pathway...
  25. doi request reprint Significance of sulfiredoxin/peroxiredoxin and mitochondrial respiratory chain in response to and protection from 100% O(2) in Saccharomyces cerevisiae
    Hiroki Fujiwara
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    Mitochondrion 13:52-8. 2013
    ....
  26. doi request reprint Transcriptional and metabolic response in yeast Saccharomyces cerevisiae cells during polyethylene glycol-dependent transformation
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    J Basic Microbiol 49:73-81. 2009
    ....
  27. ncbi request reprint Crystallization and preliminary X-ray analysis of inorganic polyphosphate/ATP-glucomannokinase from Arthrobacter sp. strain KM
    Takako Mukai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Acta Crystallogr D Biol Crystallogr 59:1662-4. 2003
    ..2, b = 83.7, c = 103.8 A. Assuming two molecules per asymmetric unit, V(sol) is 0.49. X-ray diffraction data to 2.83 A have been collected from a single crystal...
  28. ncbi request reprint Molecular insights on DNA delivery into Saccharomyces cerevisiae
    Shigeyuki Kawai
    Department of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 317:100-7. 2004
    ..We also propose that DNA is taken up via an endocytosis-like event, being at least partially different from well-known endocytosis...
  29. doi request reprint Visualization of the synergistic effect of lithium acetate and single-stranded carrier DNA on Saccharomyces cerevisiae transformation
    Tuan Anh Pham
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611 0011, Japan
    Curr Genet 57:233-9. 2011
    ..Together, the two compounds synergistically enhance the transformation efficiency and frequency...
  30. pmc Strategies for the production of high concentrations of bioethanol from seaweeds: production of high concentrations of bioethanol from seaweeds
    Mitsunori Yanagisawa
    Laboratory of Basic and Applied Molecular Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Bioengineered 4:224-35. 2013
    ..This review provides valuable information necessary for the production of high concentrations of ethanol from seaweeds. ..
  31. doi request reprint Production of ethanol from mannitol by the yeast strain Saccharomyces paradoxus NBRC 0259
    Anri Ota
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biosci Bioeng 116:327-32. 2013
    ..angophorae and E. coli KO11. As a result, we concluded that S. paradoxus NBRC 0259-3 strain is the most suitable yeast strain for the production of ethanol from mannitol. ..
  32. pmc Conferring the ability to utilize inorganic polyphosphate on ATP-specific NAD kinase
    Yusuke Nakamichi
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Sci Rep 3:2632. 2013
    ..Moreover, based on our results, we provide insight into the evolution of bacterial NADKs, in particular, how NADKs evolved from poly(P)/ATP-NADKs into ATP-specific NADKs...
  33. pmc Characterization and molecular cloning of a novel enzyme, inorganic polyphosphate/ATP-glucomannokinase, of Arthrobacter sp. strain KM
    Takako Mukai
    Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Environ Microbiol 69:3849-57. 2003
    ..Alignment of these homologous proteins revealed seven conserved regions. The mannose and poly(P) binding sites of poly(P)/ATP-glucomannokinase are discussed...