Shingo Izawa

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Stress response in yeast mRNA export factor: reversible changes in Rat8p localization are caused by ethanol stress but not heat shock
    Reiko Takemura
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Cell Sci 117:4189-97. 2004
  2. doi request reprint Heat shock and ethanol stress provoke distinctly different responses in 3'-processing and nuclear export of HSP mRNA in Saccharomyces cerevisiae
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611 0011, Japan
    Biochem J 414:111-9. 2008
  3. doi request reprint Vacuolar morphology of Saccharomyces cerevisiae during the process of wine making and Japanese sake brewing
    Shingo Izawa
    Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606 8585, Japan
    Appl Microbiol Biotechnol 88:277-82. 2010
  4. ncbi request reprint Improvement of tolerance to freeze-thaw stress of baker's yeast by cultivation with soy peptides
    Shingo Izawa
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611 0011, Japan
    Appl Microbiol Biotechnol 75:533-7. 2007
  5. ncbi request reprint Msn2p/Msn4p-activation is essential for the recovery from freezing stress in yeast
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 352:750-5. 2007
  6. ncbi request reprint Asr1, an alcohol-responsive factor of Saccharomyces cerevisiae, is dispensable for alcoholic fermentation
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Microbiol Biotechnol 72:560-5. 2006
  7. pmc Characterization of the export of bulk poly(A)+ mRNA in Saccharomyces cerevisiae during the wine-making process
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Environ Microbiol 71:2179-82. 2005
  8. ncbi request reprint Characterization of Rat8 localization and mRNA export in Saccharomyces cerevisiae during the brewing of Japanese sake
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Microbiol Biotechnol 69:86-91. 2005
  9. ncbi request reprint Formation of cytoplasmic P-bodies in sake yeast during Japanese sake brewing and wine making
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Japan
    Biosci Biotechnol Biochem 71:2800-7. 2007
  10. ncbi request reprint Gle2p is essential to induce adaptation of the export of bulk poly(A)+ mRNA to heat shock in Saccharomyces cerevisiae
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 279:35469-78. 2004

Collaborators

Detail Information

Publications38

  1. ncbi request reprint Stress response in yeast mRNA export factor: reversible changes in Rat8p localization are caused by ethanol stress but not heat shock
    Reiko Takemura
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Cell Sci 117:4189-97. 2004
    ....
  2. doi request reprint Heat shock and ethanol stress provoke distinctly different responses in 3'-processing and nuclear export of HSP mRNA in Saccharomyces cerevisiae
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611 0011, Japan
    Biochem J 414:111-9. 2008
    ..Furthermore, these results also suggest that hyperadenylation and nuclear retention of mRNAs might be used as a means to control eukaryotic gene expression under stressed conditions...
  3. doi request reprint Vacuolar morphology of Saccharomyces cerevisiae during the process of wine making and Japanese sake brewing
    Shingo Izawa
    Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606 8585, Japan
    Appl Microbiol Biotechnol 88:277-82. 2010
    ....
  4. ncbi request reprint Improvement of tolerance to freeze-thaw stress of baker's yeast by cultivation with soy peptides
    Shingo Izawa
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611 0011, Japan
    Appl Microbiol Biotechnol 75:533-7. 2007
    ..These findings promote the utilization of soy peptides as ingredients of culture media to improve the quality of baker's yeast...
  5. ncbi request reprint Msn2p/Msn4p-activation is essential for the recovery from freezing stress in yeast
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 352:750-5. 2007
    ..Since almost no expression of Msn2p/Msn4p-target genes was induced before thawing, these results indicate that Msn2p and Msn4p play a role during the recovery process from freezing...
  6. ncbi request reprint Asr1, an alcohol-responsive factor of Saccharomyces cerevisiae, is dispensable for alcoholic fermentation
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Microbiol Biotechnol 72:560-5. 2006
    ..In addition, we could not reconfirm the hypersensitivity of Asr1-deficient mutants to alcohol and sodium dodecyl sulfate. Instead, we conclude that Asr1 is not required and nor important for tolerance to alcohol stress...
  7. pmc Characterization of the export of bulk poly(A)+ mRNA in Saccharomyces cerevisiae during the wine-making process
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Environ Microbiol 71:2179-82. 2005
    ..Here we characterized the transport of mRNA during wine making and found that bulk poly(A)+ mRNA accumulated in the nucleus as fermentation progressed...
  8. ncbi request reprint Characterization of Rat8 localization and mRNA export in Saccharomyces cerevisiae during the brewing of Japanese sake
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Microbiol Biotechnol 69:86-91. 2005
    ..These results provide new information about the transport of mRNA in yeast cells during actual alcoholic fermentation...
  9. ncbi request reprint Formation of cytoplasmic P-bodies in sake yeast during Japanese sake brewing and wine making
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Japan
    Biosci Biotechnol Biochem 71:2800-7. 2007
    ..These findings suggest differences in the rate of translation and the cytoplasmic mRNA flux during the sake brewing and wine making processes...
  10. ncbi request reprint Gle2p is essential to induce adaptation of the export of bulk poly(A)+ mRNA to heat shock in Saccharomyces cerevisiae
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 279:35469-78. 2004
    ....
  11. doi request reprint Calcineurin/Crz1 destabilizes Msn2 and Msn4 in the nucleus in response to Ca(2+) in Saccharomyces cerevisiae
    Yoshifumi Takatsume
    Kyoto University, Uji, Japan
    Biochem J 427:275-87. 2010
    ..We propose that Crz1 destabilizes Msn2/Msn4 in the nuclei of cells in response to Ca2+ signalling...
  12. pmc Green tea polyphenols function as prooxidants to activate oxidative-stress-responsive transcription factors in yeasts
    Kazuhiro Maeta
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Environ Microbiol 73:572-80. 2007
    ..Additionally, we show that EGCG and GTE produce H(2)O(2) in a weak alkaline medium. Finally, we conclude that tea polyphenols are able to act as prooxidants to cause a response to oxidative stress in yeasts under certain conditions...
  13. doi request reprint Methylglyoxal activates Gcn2 to phosphorylate eIF2alpha independently of the TOR pathway in Saccharomyces cerevisiae
    Wataru Nomura
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611 0011, Japan
    Appl Microbiol Biotechnol 86:1887-94. 2010
    ..We determined the correlation between methylglyoxal and TOR kinase activity and found that phosphorylation of eIF2alpha by methylglyoxal occurred independently of the target of rapamycin (TOR) pathway...
  14. doi request reprint Role of Gcn4 for adaptation to methylglyoxal in Saccharomyces cerevisiae: methylglyoxal attenuates protein synthesis through phosphorylation of eIF2alpha
    Wataru Nomura
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 376:738-42. 2008
    ..We found that adaptation to methylglyoxal was impaired in gcn4Delta cells, indicating the expression of certain genes regulated by Gcn4 to be important for the adaptive response to methylglyoxal...
  15. ncbi request reprint Release of thioredoxin from Saccharomyces cerevisiae with environmental stimuli: solubilization of thioredoxin with ethanol
    Yoko Takeuchi
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Microbiol Biotechnol 75:1393-9. 2007
    ..The intracellular localization of thioredoxin may influence the efficiency of its release from yeast cells with ethanol...
  16. ncbi request reprint Distinct regulatory mechanism of yeast GPX2 encoding phospholipid hydroperoxide glutathione peroxidase by oxidative stress and a calcineurin/Crz1-mediated Ca2+ signaling pathway
    Daisuke Tsuzi
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    FEBS Lett 569:301-6. 2004
    ..Neither Yap1 nor Skn7 was required for Ca(2+)-dependent induction of GPX2, therefore, distinct regulation for the oxidative stress response and Ca(2+) signal response for GPX2 exists in yeast cells...
  17. pmc Efficient extraction of thioreodoxin from Saccharomyces cerevisiae by ethanol
    Yoshiharu Inoue
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Appl Environ Microbiol 73:1672-5. 2007
    ..We also found that Japanese sake contains a considerable amount of thioredoxin...
  18. doi request reprint Regulatory mechanism for expression of GPX1 in response to glucose starvation and Ca in Saccharomyces cerevisiae: involvement of Snf1 and Ras/cAMP pathway in Ca signaling
    Takumi Ohdate
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Genes Cells 15:59-75. 2010
    ..As the timing of the initiation of Ca(2+)-induced expression of GPX1 was retarded in an snf1Delta mutant, the activation of Snf1 seems pivotal to the early-stage-response of GPX1 to Ca(2+)...
  19. ncbi request reprint Unique regulation of glyoxalase I activity during osmotic stress response in the fission yeast Schizosaccharomyces pombe: neither the mRNA nor the protein level of glyoxalase I increase under conditions that enhance its activity
    Yoshifumi Takatsume
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Arch Microbiol 183:224-7. 2005
    ..In addition, glyoxalase I activity was increased in stress-activated protein kinase-deficient mutants (wis1 and spc1). We present evidence for the post-translational regulation of glyoxalase I by osmotic stress in the fission yeast...
  20. ncbi request reprint Regulation of the yeast phospholipid hydroperoxide glutathione peroxidase GPX2 by oxidative stress is mediated by Yap1 and Skn7
    Daisuke Tsuzi
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    FEBS Lett 565:148-54. 2004
    ..We determine the optimal sequence for Skn7 to regulate GPX2 under conditions of oxidative stress to be 5'-GGC(C/T)GGC-3', and we designate this sequence the oxidative stress-responsive Skn7 response element...
  21. ncbi request reprint Deficiency in the glycerol channel Fps1p confers increased freeze tolerance to yeast cells: application of the fps1delta mutant to frozen dough technology
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611 0011, Japan
    Appl Microbiol Biotechnol 66:303-5. 2004
    ..These results suggest that the fps1delta mutant is a useful strain for developing better frozen-dough with a commercial advantage...
  22. ncbi request reprint Methylglyoxal as a signal initiator for activation of the stress-activated protein kinase cascade in the fission yeast Schizosaccharomyces pombe
    Yoshifumi Takatsume
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 281:9086-92. 2006
    ..pombe has an alternative module(s) that directs the MG signal to the SAPK pathway via Mcs4. Additionally, we found that the transcription factor Pap1 is concentrated in the nucleus in response to MG, independent of the Spc1-SAPK pathway...
  23. pmc Activity of the Yap1 transcription factor in Saccharomyces cerevisiae is modulated by methylglyoxal, a metabolite derived from glycolysis
    Kazuhiro Maeta
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Mol Cell Biol 24:8753-64. 2004
    ..Moreover, we show that nucleocytoplasmic localization of Yap1 closely correlates with growth phase and intracellular MG level. We propose a novel regulatory pathway underlying Yap1 activation by a natural metabolite in the cell...
  24. ncbi request reprint Methylglyoxal, a metabolite derived from glycolysis, functions as a signal initiator of the high osmolarity glycerol-mitogen-activated protein kinase cascade and calcineurin/Crz1-mediated pathway in Saccharomyces cerevisiae
    Kazuhiro Maeta
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 280:253-60. 2005
    ..Moreover, MG activated the uptake of Ca(2+) in yeast cells, thereby stimulating the calcineurin/Crz1-mediated Ca(2+) signaling pathway. We propose that MG functions as a signal initiator in yeast...
  25. ncbi request reprint Modulation of Spc1 stress-activated protein kinase activity by methylglyoxal through inhibition of protein phosphatase in the fission yeast Schizosaccharomyces pombe
    Yoshifumi Takatsume
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 363:942-7. 2007
    ..In addition, we found that methylglyoxal inhibits human protein tyrosine phosphatase 1B (PTP1B) also. We propose a model for the regulation of the activity of the Spc1-SAPK signaling pathway by methylglyoxal in S. pombe...
  26. ncbi request reprint GPX2, encoding a phospholipid hydroperoxide glutathione peroxidase homologue, codes for an atypical 2-Cys peroxiredoxin in Saccharomyces cerevisiae
    Tomoaki Tanaka
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 280:42078-87. 2005
    ..Gpx2 is likely to play an important role in the protection of cells from oxidative stress in the presence of Ca2+...
  27. ncbi request reprint Enrichment of yeast thioredoxin by green tea extract through activation of Yap1 transcription factor in Saccharomyces cerevisiae
    Yoshifumi Takatsume
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Agric Food Chem 53:332-7. 2005
    ..Maximal production of TRX was achieved in a medium containing 0.1% green tea extract at pH 7.6. We discuss the underlying mechanism by which green tea extract activates Yap1...
  28. pmc Reduction of glucose uptake through inhibition of hexose transporters and enhancement of their endocytosis by methylglyoxal in Saccharomyces cerevisiae
    Aya Yoshida
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 287:701-11. 2012
    ..Meanwhile, the internalization of hexose transporters following treatment with methylglyoxal was delayed in a mutant defective in protein kinase C...
  29. ncbi request reprint Diagnosis of cell death induced by methylglyoxal, a metabolite derived from glycolysis, in Saccharomyces cerevisiae
    Kazuhiro Maeta
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    FEMS Microbiol Lett 243:87-92. 2005
    ..We demonstrate that MG at high concentrations provokes necrotic cell death without the generation of reactive oxygen species in S. cerevisiae...
  30. doi request reprint Modification of yeast characteristics by soy peptides: cultivation with soy peptides represses the formation of lipid bodies
    Kayo Ikeda
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan
    Appl Microbiol Biotechnol 89:1971-7. 2011
    ..These results indicate that soy peptides affect the lipid metabolism in yeast cells, and also demonstrate a potentiality of edible natural ingredients as modifiers of the characteristics of food microorganisms...
  31. pmc Vanillin inhibits translation and induces messenger ribonucleoprotein (mRNP) granule formation in saccharomyces cerevisiae: application and validation of high-content, image-based profiling
    Aya Iwaki
    the Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan
    PLoS ONE 8:e61748. 2013
    ..Processing bodies and stress granules, which are composed of non-translating mRNAs and various proteins, were formed after treatment with vanillin. These results suggest that vanillin represses translation in yeast cells...
  32. ncbi request reprint Intracellular glycerol influences resistance to freeze stress in Saccharomyces cerevisiae: analysis of a quadruple mutant in glycerol dehydrogenase genes and glycerol-enriched cells
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto 611 0011, Japan
    Appl Microbiol Biotechnol 66:108-14. 2004
    ..These results suggest the possibility of using intracellular-glycerol-enriched cells to develop better frozen dough...
  33. pmc Biomass conversion inhibitors furfural and 5-hydroxymethylfurfural induce formation of messenger RNP granules and attenuate translation activity in Saccharomyces cerevisiae
    Aya Iwaki
    Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan
    Appl Environ Microbiol 79:1661-7. 2013
    ....
  34. doi request reprint Temperature dependent N-glycosylation of plasma membrane heat shock protein Hsp30p in Saccharomyces cerevisiae
    Ken ichi Kamo
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 420:119-23. 2012
    ..Since limited information is available on stress-responsive or condition-induced N-glycosylation, our findings provide new insight into the regulation of cellular stress response in yeast...
  35. doi request reprint Severe ethanol stress induces assembly of stress granules in Saccharomyces cerevisiae
    Kenta Kato
    Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan
    Yeast 28:339-47. 2011
    ....
  36. doi request reprint Post-transcriptional regulation of gene expression in yeast under ethanol stress
    Shingo Izawa
    Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Gokasho, Uji City, Kyoto 611 0011, Japan
    Biotechnol Appl Biochem 53:93-9. 2009
    ..The present minireview outlines the regulation of yeast gene expression at the mRNA-processing and nuclear-export steps under ethanol-stressed conditions, highlighting their usefulness in the brewing industry...
  37. ncbi request reprint Identification of thermostable glyoxalase I in the fission yeast Schizosaccharomyces pombe
    Yoshifumi Takatsume
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Arch Microbiol 181:371-7. 2004
    ..The glyoxalase I of S. pombe exhibited fairly high thermal stability, and almost 100% activity was retained after incubating the enzyme at 60 degrees C for 4 h...
  38. doi request reprint Adaptive response in stress granule formation and bulk translational repression upon a combined stress of mild heat shock and mild ethanol stress in yeast
    Yosuke Yamamoto
    Laboratory of Microbial Technology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto, 606 8585, Japan
    Genes Cells 18:974-84. 2013
    ..However, the pretreated cells still exhibited a severe repression of translation activity. These findings provide novel and fundamental insight into the regulation of yeast stress granules. ..