Kouji Hirota

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. doi request reprint Stepwise chromatin remodelling by a cascade of transcription initiation of non-coding RNAs
    Kouji Hirota
    Cellular and Molecular Biology Laboratory, RIKEN Advanced Science Institute, Wako Shi, Saitama 351 0198, Japan
    Nature 456:130-4. 2008
  2. pmc Interference in DNA replication can cause mitotic chromosomal breakage unassociated with double-strand breaks
    Mari Fujita
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshidakonoe, Sakyo ku, Kyoto, Japan
    PLoS ONE 8:e60043. 2013
  3. ncbi request reprint Cascade transcription of mRNA-type long non-coding RNAs (mlonRNAs) and local chromatin remodeling
    Kouji Hirota
    Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Yoshida Konoe, Sakyo ku, Japan
    Epigenetics 4:5-7. 2009
  4. ncbi request reprint Compensatory functions and interdependency of the DNA-binding domain of BRCA2 with the BRCA1-PALB2-BRCA2 complex
    Muthana Al Abo
    Authors Affiliations Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Sakyo ku and Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku ku, Tokyo, Japan
    Cancer Res 74:797-807. 2014
  5. pmc Fission yeast global repressors regulate the specificity of chromatin alteration in response to distinct environmental stresses
    Kouji Hirota
    Genetic Dynamics Research Unit Laboratory, RIKEN Institute of Physical and Chemical Research, Wako Shi, Saitama 351 0198, Japan
    Nucleic Acids Res 32:855-62. 2004
  6. pmc The epistatic relationship between BRCA2 and the other RAD51 mediators in homologous recombination
    Yong Qing
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    PLoS Genet 7:e1002148. 2011
  7. pmc Reciprocal nuclear shuttling of two antagonizing Zn finger proteins modulates Tup family corepressor function to repress chromatin remodeling
    Kouji Hirota
    Genetic System Regulation Laboratory, RIKEN The Institute of Physical and Chemical Research, Discovery Research Institute, Wako Shi, Saitama 351 0198, Japan
    Eukaryot Cell 5:1980-9. 2006
  8. pmc Distinct chromatin modulators regulate the formation of accessible and repressive chromatin at the fission yeast recombination hotspot ade6-M26
    Kouji Hirota
    Shibata Distinguished Senior Scientist Laboratory, RIKEN Discovery Research Institute, Wako Shi, Saitama 351 0198, Japan
    Mol Biol Cell 19:1162-73. 2008
  9. pmc Involvement of SLX4 in interstrand cross-link repair is regulated by the Fanconi anemia pathway
    Kimiyo N Yamamoto
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 108:6492-6. 2011
  10. pmc Characterization of environmental chemicals with potential for DNA damage using isogenic DNA repair-deficient chicken DT40 cell lines
    Kimiyo N Yamamoto
    Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshidakonoe, Sakyo, Japan
    Environ Mol Mutagen 52:547-61. 2011

Collaborators

Detail Information

Publications21

  1. doi request reprint Stepwise chromatin remodelling by a cascade of transcription initiation of non-coding RNAs
    Kouji Hirota
    Cellular and Molecular Biology Laboratory, RIKEN Advanced Science Institute, Wako Shi, Saitama 351 0198, Japan
    Nature 456:130-4. 2008
    ..Our results demonstrate that transcription through the promoter region is required to make DNA sequences accessible to transcriptional activators and to RNAPII...
  2. pmc Interference in DNA replication can cause mitotic chromosomal breakage unassociated with double-strand breaks
    Mari Fujita
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshidakonoe, Sakyo ku, Kyoto, Japan
    PLoS ONE 8:e60043. 2013
    ....
  3. ncbi request reprint Cascade transcription of mRNA-type long non-coding RNAs (mlonRNAs) and local chromatin remodeling
    Kouji Hirota
    Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Yoshida Konoe, Sakyo ku, Japan
    Epigenetics 4:5-7. 2009
    ..Here, we hypothesize that RNAPII transcription of mlonRNAs disrupts chromatin array. This idea may provide a new clue to understand why so many species of ncRNAs are expressed in eukaryotic cells...
  4. ncbi request reprint Compensatory functions and interdependency of the DNA-binding domain of BRCA2 with the BRCA1-PALB2-BRCA2 complex
    Muthana Al Abo
    Authors Affiliations Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Sakyo ku and Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, Shinjuku ku, Tokyo, Japan
    Cancer Res 74:797-807. 2014
    ..This knowledge adds to our ability to accurately predict the efficacy of antimalignant therapies for patients carrying mutations in the BRCA2 gene...
  5. pmc Fission yeast global repressors regulate the specificity of chromatin alteration in response to distinct environmental stresses
    Kouji Hirota
    Genetic Dynamics Research Unit Laboratory, RIKEN Institute of Physical and Chemical Research, Wako Shi, Saitama 351 0198, Japan
    Nucleic Acids Res 32:855-62. 2004
    ..Such a role for these proteins may serve as a paradigm for the regulation of stress response in higher eukaryotes...
  6. pmc The epistatic relationship between BRCA2 and the other RAD51 mediators in homologous recombination
    Yong Qing
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    PLoS Genet 7:e1002148. 2011
    ..We suggest that BRCA2 might thus serve as a platform to recruit various RAD51 mediators at the appropriate position at the DNA-damage site...
  7. pmc Reciprocal nuclear shuttling of two antagonizing Zn finger proteins modulates Tup family corepressor function to repress chromatin remodeling
    Kouji Hirota
    Genetic System Regulation Laboratory, RIKEN The Institute of Physical and Chemical Research, Discovery Research Institute, Wako Shi, Saitama 351 0198, Japan
    Eukaryot Cell 5:1980-9. 2006
    ..This notion provides new insights into the molecular mechanisms of the Tup family corepressors in gene regulation...
  8. pmc Distinct chromatin modulators regulate the formation of accessible and repressive chromatin at the fission yeast recombination hotspot ade6-M26
    Kouji Hirota
    Shibata Distinguished Senior Scientist Laboratory, RIKEN Discovery Research Institute, Wako Shi, Saitama 351 0198, Japan
    Mol Biol Cell 19:1162-73. 2008
    ..Thus, we propose that multiple chromatin modulators regulate chromatin structure reversibly and participate in the regulation of both meiotic recombination and stress-induced transcription around CRE-like sequences...
  9. pmc Involvement of SLX4 in interstrand cross-link repair is regulated by the Fanconi anemia pathway
    Kimiyo N Yamamoto
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 108:6492-6. 2011
    ..Our findings thus suggest that ubiquitylated FANCD2 recruits SLX4 to DNA damage sites, where it mediates the resolution of recombination intermediates generated during the processing of ICLs...
  10. pmc Characterization of environmental chemicals with potential for DNA damage using isogenic DNA repair-deficient chicken DT40 cell lines
    Kimiyo N Yamamoto
    Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshidakonoe, Sakyo, Japan
    Environ Mol Mutagen 52:547-61. 2011
    ..The results demonstrate the utility of this DT40 screen for detecting genotoxic compounds, for characterizing the nature of the DNA damage, and potentially for analyzing mechanisms of mutagenesis...
  11. pmc Fission yeast Tup1-like repressors repress chromatin remodeling at the fbp1+ promoter and the ade6-M26 recombination hotspot
    Kouji Hirota
    Genetic Dynamics Research Unit Laboratory, The Institute of Physical and Chemical Research RIKEN, Wako Shi, Saitama 351 0198, Japan
    Genetics 165:505-15. 2003
    ..These observations suggest that these fission yeast Tup1-like corepressors repress chromatin remodeling at CRE-related sequences and that Rst2 antagonizes this function...
  12. pmc Structure-specific endonucleases xpf and mus81 play overlapping but essential roles in DNA repair by homologous recombination
    Koji Kikuchi
    Department of Radiation Genetics, and Frontier Technology Center, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto, Japan
    Cancer Res 73:4362-71. 2013
    ..In summary, Xpf and Mus81 have a substantially overlapping function in completion of HR...
  13. pmc KIAA1018/FAN1 nuclease protects cells against genomic instability induced by interstrand cross-linking agents
    Kazunori Yoshikiyo
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 107:21553-7. 2010
    ....
  14. doi request reprint Mutant cells defective in DNA repair pathways provide a sensitive high-throughput assay for genotoxicity
    Terry John Evans
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshidakonoe, Sakyo ku, Kyoto 606 8501, Japan
    DNA Repair (Amst) 9:1292-8. 2010
    ..Our panel of mutants is likely to be useful in screening large compound libraries for an emerging class of chemotherapeutic drugs, which includes inhibitors of DNA repair enzymes such as PARP and DNA polymerases...
  15. pmc Bloom DNA helicase facilitates homologous recombination between diverged homologous sequences
    Koji Kikuchi
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshidakonoe, Kyoto 606 8501, Japan
    J Biol Chem 284:26360-7. 2009
    ..Conversely, the overexpression of Blm, even an ATPase-defective mutant, strongly stimulated gene targeting. In summary, Blm promotes HR between diverged sequences through a novel ATPase-independent mechanism...
  16. doi request reprint A novel genotoxicity assay of carbon nanotubes using functional macrophage receptor with collagenous structure (MARCO)-expressing chicken B lymphocytes
    - Mohiuddin
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto, 606 8501, Japan
    Arch Toxicol 88:145-60. 2014
    ..Taken together, our novel assay system using the chimeric receptor-expressing DT40 cells provides a sensitive method to screen for genotoxicity of CNTs and possibly other nanomaterials. ..
  17. pmc The USP1/UAF1 complex promotes double-strand break repair through homologous recombination
    Junko Murai
    Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Mol Cell Biol 31:2462-9. 2011
    ..Disruption of NHEJ in UAF1-deficient cells restored cellular resistance to camptothecin and the PARP inhibitor. Our results indicate that the USP1/UAF1 complex promotes HR, at least in part by suppressing NHEJ...
  18. pmc DNA polymerases nu and theta are required for efficient immunoglobulin V gene diversification in chicken
    Masaoki Kohzaki
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 189:1117-27. 2010
    ..Together, these data define a role for Polnu and Pol in recombination and suggest that the DNA synthesis associated with Ig gene conversion is accounted for by three specialized DNA polymerases...
  19. pmc The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination
    Yuichiro Saito
    Division of Genome Repair Dynamics, Radiation Biology Center, Kyoto University, Yoshida Konoecho, Sakyo ku, Kyoto, Japan
    Sci Rep 3:2022. 2013
    ..Rad22 physically interacted with the BAG domain of Bag101, and a lack of this domain enhanced HR frequency. Similarly, radiation exposure triggered the dissociation of these proteins so that Rad22 was stabilized and able to enhance HR...
  20. doi request reprint Human replicative DNA polymerase δ can bypass T-T (6-4) ultraviolet photoproducts on template strands
    Takeo Narita
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshidakonoe, Sakyo ku, Kyoto 606 8501, Japan
    Genes Cells 15:1228-39. 2010
    ..This unexpected enzymatic property of Polδ in the bypass of UV photoproducts challenges the received notion that the accuracy of Polδ prevents bypassing damaged templates...
  21. pmc C-terminal region of activation-induced cytidine deaminase (AID) is required for efficient class switch recombination and gene conversion
    Somayeh Sabouri
    Departments of Immunology and Genomic Medicine and Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida Sakyo ku, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 111:2253-8. 2014
    ..Collectively, our data indicate that the C-terminal region of AID is required for efficient generation of DSB in CSR and GC and thus for the subsequent pairing of cleaved DNA ends during recombination in CSR. ..