Hiroaki Higashitsuji

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. pmc Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells
    Yasuhiko Sumitomo
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    BMC Biotechnol 12:72. 2012
  2. ncbi request reprint The oncoprotein gankyrin interacts with RelA and suppresses NF-kappaB activity
    Hiroaki Higashitsuji
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 363:879-84. 2007
  3. ncbi request reprint A cleaved form of MAGE-A4 binds to Miz-1 and induces apoptosis in human cells
    Toshiharu Sakurai
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    J Biol Chem 279:15505-14. 2004
  4. ncbi request reprint The oncoprotein gankyrin binds to MDM2/HDM2, enhancing ubiquitylation and degradation of p53
    Hiroaki Higashitsuji
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    Cancer Cell 8:75-87. 2005
  5. ncbi request reprint Enhanced deacetylation of p53 by the anti-apoptotic protein HSCO in association with histone deacetylase 1
    Hisako Higashitsuji
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Kyoto 606 8507, Japan
    J Biol Chem 282:13716-25. 2007
  6. ncbi request reprint Association of gankyrin protein expression with early clinical stages and insulin-like growth factor-binding protein 5 expression in human hepatocellular carcinoma
    Atsushi Umemura
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Hepatology 47:493-502. 2008
  7. ncbi request reprint Apg-2 has a chaperone-like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas
    Kazuhisa Gotoh
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8507, Japan
    FEBS Lett 560:19-24. 2004
  8. ncbi request reprint Engineered long terminal repeats of retroviral vectors enhance transgene expression in hepatocytes in vitro and in vivo
    Kanji Yamaguchi
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 606 8507, Kyoto, Japan
    Mol Ther 8:796-803. 2003
  9. ncbi request reprint MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity
    Toshikazu Nagao
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Japan
    J Biol Chem 278:10668-74. 2003
  10. pmc Cold-inducible RNA-binding protein (Cirp) interacts with Dyrk1b/Mirk and promotes proliferation of immature male germ cells in mice
    Tomoko Masuda
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 109:10885-90. 2012

Collaborators

Detail Information

Publications18

  1. pmc Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells
    Yasuhiko Sumitomo
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    BMC Biotechnol 12:72. 2012
    ..To clarify the molecular mechanism underlying the induction and to exploit this to improve the productivity of recombinant proteins, we tried to identify the regulatory sequence(s) in the 5' flanking region of the mouse cirp gene...
  2. ncbi request reprint The oncoprotein gankyrin interacts with RelA and suppresses NF-kappaB activity
    Hiroaki Higashitsuji
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 363:879-84. 2007
    ..Thus, gankyrin binds to NF-kappaB and suppresses its activity at the transcription level by modulating acetylation via SIRT1...
  3. ncbi request reprint A cleaved form of MAGE-A4 binds to Miz-1 and induces apoptosis in human cells
    Toshiharu Sakurai
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    J Biol Chem 279:15505-14. 2004
    ..Furthermore, the finding that a C-terminal fragment with pro-apoptotic activity is generated from full-length MAGE-A4 after genotoxic stress in human cells suggests a novel function for MAGE-A4...
  4. ncbi request reprint The oncoprotein gankyrin binds to MDM2/HDM2, enhancing ubiquitylation and degradation of p53
    Hiroaki Higashitsuji
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    Cancer Cell 8:75-87. 2005
    ..Downregulation of gankyrin reduced amounts of Mdm2 and p53 associated with the 26S proteasome. Thus, gankyrin is a cofactor that increases the activities of Mdm2 on p53 and probably targets polyubiquitylated p53 into the 26S proteasome...
  5. ncbi request reprint Enhanced deacetylation of p53 by the anti-apoptotic protein HSCO in association with histone deacetylase 1
    Hisako Higashitsuji
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Kyoto 606 8507, Japan
    J Biol Chem 282:13716-25. 2007
    ..Treatment of hepatocellular carcinomas that retain wild-type p53 and overexpress HSCO with anti-HSCO agents might re-establish the p53 response and revert chemoresistance...
  6. ncbi request reprint Association of gankyrin protein expression with early clinical stages and insulin-like growth factor-binding protein 5 expression in human hepatocellular carcinoma
    Atsushi Umemura
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Hepatology 47:493-502. 2008
    ..629, P < 0.001). CONCLUSION: These results suggest that gankyrin plays an oncogenic role(s) mainly at the early stages of human hepatocarcinogenesis, and that IGFBP-5 inducible by gankyrin overexpression may be involved in it...
  7. ncbi request reprint Apg-2 has a chaperone-like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas
    Kazuhisa Gotoh
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8507, Japan
    FEBS Lett 560:19-24. 2004
    ..These results suggest that the Hsp110 family members play important roles in hepatocarcinogenesis through their chaperoning activities...
  8. ncbi request reprint Engineered long terminal repeats of retroviral vectors enhance transgene expression in hepatocytes in vitro and in vivo
    Kanji Yamaguchi
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 606 8507, Kyoto, Japan
    Mol Ther 8:796-803. 2003
    ..5- to 5-fold in comparison with a Moloney murine leukemia virus-based vector or a vector containing the wild-type LTR of SFFVp, respectively, in murine hepatocytes in vivo...
  9. ncbi request reprint MAGE-A4 interacts with the liver oncoprotein gankyrin and suppresses its tumorigenic activity
    Toshikazu Nagao
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Japan
    J Biol Chem 278:10668-74. 2003
    ..So far, the major focus of studies on the MAGE proteins has been on their potential for cancer immunotherapy. Our results may also shed light on novel functions for MAGE-A proteins...
  10. pmc Cold-inducible RNA-binding protein (Cirp) interacts with Dyrk1b/Mirk and promotes proliferation of immature male germ cells in mice
    Tomoko Masuda
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 109:10885-90. 2012
    ..Thus, our study demonstrates that Cirp functions to fine-tune the proliferation of undifferentiated spermatogonia by interacting with Dyrk1b...
  11. ncbi request reprint Gankyrin oncoprotein overexpression as a critical factor for tumor growth in human esophageal squamous cell carcinoma and its clinical significance
    Cristian M Ortiz
    Department of Surgery, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto, Japan
    Int J Cancer 122:325-32. 2008
    ..Gankyrin overexpression is associated with poor prognosis. It may play an important role in ESCC tumor progression and could be a potentially important therapeutic gene target in ESCC...
  12. doi request reprint Overexpression of gankyrin in mouse hepatocytes induces hemangioma by suppressing factor inhibiting hypoxia-inducible factor-1 (FIH-1) and activating hypoxia-inducible factor-1
    Yu Liu
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 432:22-7. 2013
    ..Thus, the present study clarified, at least partly, mechanisms of vascular tumorigenesis, and suggests that gankyrin might play a physiological role in hypoxic responses besides its roles as an oncoprotein...
  13. ncbi request reprint Low temperature protects mammalian cells from apoptosis initiated by various stimuli in vitro
    Toshiharu Sakurai
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 606 8507, Japan
    Exp Cell Res 309:264-72. 2005
    ..In conclusion, mild hypothermia protects cells from a variety of stress by p53-dependent and p53-independent mechanisms...
  14. ncbi request reprint Cirp protects against tumor necrosis factor-alpha-induced apoptosis via activation of extracellular signal-regulated kinase
    Toshiharu Sakurai
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, Kyoto 605 8507, Japan
    Biochim Biophys Acta 1763:290-5. 2006
    ..These data suggest that mild hypothermia protects cells from TNF-alpha-induced apoptosis, at least partly, via induction of Cirp, and that Cirp protects cells by activating the ERK pathway...
  15. ncbi request reprint A novel protein overexpressed in hepatoma accelerates export of NF-kappa B from the nucleus and inhibits p53-dependent apoptosis
    Hisako Higashitsuji
    Department of Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 54 Shogoin Kawaharacho, Sakyo ku, 606 8507, Kyoto, Japan
    Cancer Cell 2:335-46. 2002
    ..These results suggest that overexpression of HSCO suppresses p53-induced apoptosis by preventing nuclear localization of NF-kappa B during signaling and thus contributes to hepatocarcinogenesis...
  16. ncbi request reprint The oncoprotein gankyrin negatively regulates both p53 and RB by enhancing proteasomal degradation
    Hiroaki Higashitsuji
    Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Cell Cycle 4:1335-7. 2005
    ..Furthermore, inhibition of gankyrin induces apoptosis in cancer cells. Therefore, gankyrin is a promising target for potential anticancer therapeutic agents...
  17. ncbi request reprint Role of a novel oncogenic protein, gankyrin, in hepatocyte proliferation
    Akio Iwai
    Division of Gastroenterology and Hepatology, Department of Medicine, Graduate School of Medicine, Kyoto University, 54 Kawara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    J Gastroenterol 38:751-8. 2003
    ..We investigated the possible involvement of gankyrin in hepatocyte proliferation and examined the expression of gankyrin in the liver of patients with fulminant hepatic failure (FHF)...
  18. pmc MDM2 is a novel E3 ligase for HIV-1 Vif
    Taisuke Izumi
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Sakyo ku, Japan
    Retrovirology 6:1. 2009
    ..These data suggest that MDM2 is a regulator of HIV-1 replication and might be a novel therapeutic target for anti-HIV-1 drug...