S Futaki

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Controlling leucine-zipper partner recognition in cells through modification of a-g interactions
    Yusuke Azuma
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Chem Commun (Camb) 50:6364-7. 2014
  2. ncbi request reprint Arginine-rich peptides: methods of translocation through biological membranes
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Curr Pharm Des 19:2863-8. 2013
  3. doi request reprint Cell-penetrating peptides (CPPs) as a vector for the delivery of siRNAs into cells
    Ikuhiko Nakase
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Mol Biosyst 9:855-61. 2013
  4. doi request reprint Dipicolylamine as a unique structural switching element for helical peptides
    Yusuke Azuma
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Org Biomol Chem 10:6062-8. 2012
  5. ncbi request reprint Structural variety of membrane permeable peptides
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Curr Protein Pept Sci 4:87-96. 2003
  6. ncbi request reprint Membrane permeability commonly shared among arginine-rich peptides
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Mol Recognit 16:260-4. 2003
  7. ncbi request reprint Unique features of a pH-sensitive fusogenic peptide that improves the transfection efficiency of cationic liposomes
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Gene Med 7:1450-8. 2005
  8. ncbi request reprint Gramicidin-based channel systems for the detection of protein-ligand interaction
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioorg Med Chem 12:1343-50. 2004
  9. ncbi request reprint Arginine carrier peptide bearing Ni(II) chelator to promote cellular uptake of histidine-tagged proteins
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioconjug Chem 15:475-81. 2004
  10. ncbi request reprint RNase S complex bearing arginine-rich peptide and anti-HIV activity
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Mol Recognit 18:169-74. 2005

Collaborators

Detail Information

Publications88

  1. ncbi request reprint Controlling leucine-zipper partner recognition in cells through modification of a-g interactions
    Yusuke Azuma
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Chem Commun (Camb) 50:6364-7. 2014
    ....
  2. ncbi request reprint Arginine-rich peptides: methods of translocation through biological membranes
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Curr Pharm Des 19:2863-8. 2013
    ..In this review, we describe our current views of membrane translocation of CPPs, especially those rich in arginine, as these peptides represent one of the most frequently employed classes of CPPs...
  3. doi request reprint Cell-penetrating peptides (CPPs) as a vector for the delivery of siRNAs into cells
    Ikuhiko Nakase
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Mol Biosyst 9:855-61. 2013
    ..This review provides an overview of the current approaches and describes the potential of CPP-based siRNA delivery systems, specifically those using arginine-rich CPPs...
  4. doi request reprint Dipicolylamine as a unique structural switching element for helical peptides
    Yusuke Azuma
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Org Biomol Chem 10:6062-8. 2012
    ..This feature was utilized for the selective detection of Ni(ii) using the peptide-Fe(ii) complex. Repeated switching of the helical structure was also achieved by multiple additions of divalent metal ions to the peptide...
  5. ncbi request reprint Structural variety of membrane permeable peptides
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Curr Protein Pept Sci 4:87-96. 2003
    ..In this review, we present the structural variety of membrane permeable peptides and provide a survey of the findings on the translocation of these peptides through the cell membranes...
  6. ncbi request reprint Membrane permeability commonly shared among arginine-rich peptides
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Mol Recognit 16:260-4. 2003
    ..The structural and mechanistic features of the translocation of these membrane-permeable arginine-rich peptides are reviewed...
  7. ncbi request reprint Unique features of a pH-sensitive fusogenic peptide that improves the transfection efficiency of cationic liposomes
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Gene Med 7:1450-8. 2005
    ..Detailed studies on the methods of enhancement would broaden the horizon of its application...
  8. ncbi request reprint Gramicidin-based channel systems for the detection of protein-ligand interaction
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioorg Med Chem 12:1343-50. 2004
    ..Thus, high-affinity ligands upon tagging to gramicidin render the gramicidin-based sensor able to execute as a real-time monitoring system for protein-ligand interaction...
  9. ncbi request reprint Arginine carrier peptide bearing Ni(II) chelator to promote cellular uptake of histidine-tagged proteins
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioconjug Chem 15:475-81. 2004
    ..The apoptosis-inducing activity of the peptide thus delivered was compared with that of the PAD peptide covalently connected with the octaarginine peptide...
  10. ncbi request reprint RNase S complex bearing arginine-rich peptide and anti-HIV activity
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Mol Recognit 18:169-74. 2005
    ..The present study exemplified the applicability of the arginine-rich peptides to the intracellular targeting of non-covalent protein complexes and supramolecular assemblies for the research in chemical and cellular biology...
  11. ncbi request reprint Oligoarginine vectors for intracellular delivery: design and cellular-uptake mechanisms
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Japan
    Biopolymers 84:241-9. 2006
    ..In this review, the structural features of these arginine-rich peptide vectors have been focused on and the current understandings of their internalization mechanisms are summarized...
  12. ncbi request reprint Membrane-permeable arginine-rich peptides and the translocation mechanisms
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan Presto, Japan Science and Technology Corporation JST, Kawaguchi, Saitama 332 0012, Japan
    Adv Drug Deliv Rev 57:547-58. 2005
    ..This review summarizes the structures of these peptide vectors, especially the Tat and other arginine-rich peptides, and the current understanding of their internalization mechanisms...
  13. ncbi request reprint Arginine-rich peptides and their internalization mechanisms
    S Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Soc Trans 35:784-7. 2007
    ..This review summarizes the current theories on both endocytic and non-endocytic aspects of internalization of arginine-rich peptides...
  14. ncbi request reprint Ligand-induced extramembrane conformation switch controlling alamethicin assembly and the channel current
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan
    Chem Biodivers 4:1313-22. 2007
    ..Therefore, conformational changes in the extramembrane segments induced by the interaction with ligands can be reflected in the current levels...
  15. ncbi request reprint Arginine-rich peptides: potential for intracellular delivery of macromolecules and the mystery of the translocation mechanisms
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Int J Pharm 245:1-7. 2002
    ..In this review, the potential of these peptides for the intracellular delivery of macromolecules and the mystery of the translocation mechanisms are reviewed...
  16. ncbi request reprint [Membrane permeable basic peptides: the potential of these peptides for the intracellular delivery of proteins and drugs]
    Shiroh Futaki
    Tanpakushitsu Kakusan Koso 47:1415-9. 2002
  17. ncbi request reprint Alamethicin-leucine zipper hybrid peptide: a prototype for the design of artificial receptors and ion channels
    S Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Am Chem Soc 123:12127-34. 2001
    ..Employment of an extramembrane segment of a random conformation provided higher levels of channel conductance. The result exemplified the possibility of channel current control by a conformational switch of the extramembrane segments...
  18. ncbi request reprint Stearylated arginine-rich peptides: a new class of transfection systems
    S Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioconjug Chem 12:1005-11. 2001
    ..Also, a certain correlation was observed between the transfection efficiency of stearyl-(Arg)n peptides (stearyl-Rn: n = 4, 8, 12, 16) and the membrane permeability of the corresponding (Arg)n peptides (Rn)...
  19. ncbi request reprint Translocation of branched-chain arginine peptides through cell membranes: flexibility in the spatial disposition of positive charges in membrane-permeable peptides
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 41:7925-30. 2002
    ..The results strongly suggested that a linear structure was not indispensable for the translocation of arginine-rich peptides and that there could be considerable flexibility in the location of the arginine residue in the molecules...
  20. ncbi request reprint A 'cassette' RNase: site-selective cleavage of RNA by RNase S equipped with RNA-recognition segment
    S Futaki
    Institute for Chemical Research, Kyoto University, Uji, Japan
    Bioorg Med Chem Lett 11:1165-8. 2001
    ..The resulting RNase S bearing the RNA-recognition segment preferentially hydrolyzed a single position of the RNA stem-loop derived from the specific binding site for the Rev protein...
  21. ncbi request reprint Chemical ligation to obtain proteins comprising helices with individual amino acid sequences
    S Futaki
    Institute for Medicinal Resources, The University of Tokushima, Japan
    Bioorg Med Chem 7:187-92. 1999
    ..We will introduce our approach for the selective assembly of helical peptide segments on a peptide template to give four-helix-bundle proteins comprising individual helices...
  22. ncbi request reprint Arginine-rich peptides. An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery
    S Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 276:5836-40. 2001
    ..Using (Arg)(n) (n = 4-16) peptides, we also demonstrated that there would be an optimal number of arginine residues (n approximately 8) for the efficient translocation...
  23. doi request reprint Cellular internalization and distribution of arginine-rich peptides as a function of extracellular peptide concentration, serum, and plasma membrane associated proteoglycans
    Michie Kosuge
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioconjug Chem 19:656-64. 2008
    ..These results demonstrate that uptake mechanisms of these compounds are highly dependent on both the presence of serum and the effective extracellular peptide concentration...
  24. ncbi request reprint Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas)
    Kentaro Takayama
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Control Release 138:128-33. 2009
    ....
  25. ncbi request reprint Embodying a stable alpha-helical protein structure through efficient chemical ligation via thioether formation
    S Futaki
    Institute for Medicinal Resources, University of Tokushima, Japan
    Bioorg Med Chem 5:1883-91. 1997
    ..A trial of introducing an additional intramolecular disulfide cross-link was also examined. The resulting protein showed high stability in the chaotropic and thermal denaturation and in enzymatic degradation...
  26. ncbi request reprint Detection of protein-ligand interaction on the membranes using C-terminus biotin-tagged alamethicin
    Y Zhang
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioorg Med Chem 10:2635-9. 2002
    ..The system was also applicable to the detection of interaction of the biotin-tagged alamethicin with the anti-biotin antibody...
  27. ncbi request reprint Possible existence of common internalization mechanisms among arginine-rich peptides
    Tomoki Suzuki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Biol Chem 277:2437-43. 2002
    ..These results strongly suggest that the arginine-rich basic peptides share a certain part of the internalization pathway...
  28. ncbi request reprint Cellular uptake of arginine-rich peptides: roles for macropinocytosis and actin rearrangement
    Ikuhiko Nakase
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Mol Ther 10:1011-22. 2004
    ..The above observations suggest that penetratin and R8 peptides have distinct internalization mechanisms...
  29. doi request reprint Cytosolic targeting of macromolecules using a pH-dependent fusogenic peptide in combination with cationic liposomes
    Sachiko Kobayashi
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioconjug Chem 20:953-9. 2009
    ....
  30. pmc Cell-surface accumulation of flock house virus-derived peptide leads to efficient internalization via macropinocytosis
    Ikuhiko Nakase
    Institute for Chemical Research, Kyoto University, Kyoto, Japan
    Mol Ther 17:1868-76. 2009
    ..The remarkable methods of FHV peptide internalization thus highlighted the critical factors for internalizations of the arginine-rich CPPs...
  31. ncbi request reprint Alpha-helical linker of an artificial 6-zinc finger peptide contributes to selective DNA binding to a discontinuous recognition sequence
    Wei Yan
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 46:8517-24. 2007
    ..The engineering of a helix-structured linker in the 6-zinc finger peptides should be one of the most promising approaches for selectively targeting discontinuous recognition sites depending on their phase situations...
  32. ncbi request reprint Methodological and cellular aspects that govern the internalization mechanisms of arginine-rich cell-penetrating peptides
    Ikuhiko Nakase
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Adv Drug Deliv Rev 60:598-607. 2008
    ..Similarities and dissimilarities among the internalization methods of typical peptide vectors and other guanidino-rich vectors including branched-chain, beta-peptide, and sugar-based vectors, are also discussed...
  33. ncbi request reprint Total synthesis of artificial zinc-finger proteins: Problems and perspectives
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biopolymers 76:98-109. 2004
    ....
  34. ncbi request reprint Transmission of extramembrane conformational change into current: construction of metal-gated ion channel
    Tatsuto Kiwada
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Am Chem Soc 128:6010-1. 2006
    ..Interaction with Fe(III) induces the reversible conformational switch of the extramembrane segment in the artificial receptor channel, which is transmitted into membranes as an increase in channel current (ion flux)...
  35. ncbi request reprint Oligoarginine-based prodrugs with self-cleavable spacers for caco-2 cell permeation
    Kentaro Takayama
    Institute for Chemical Research, Kyoto University, Kyoto, Japan
    Chem Pharm Bull (Tokyo) 56:1515-20. 2008
    ....
  36. ncbi request reprint High-resolution multi-dimensional NMR spectroscopy of proteins in human cells
    Kohsuke Inomata
    Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Nishikyo ku, Kyoto 615 8510, Japan
    Nature 458:106-9. 2009
    ..Moreover, in-cell NMR spectroscopy demonstrates that ubiquitin has much higher hydrogen exchange rates in the intracellular environment, possibly due to multiple interactions with endogenous proteins...
  37. ncbi request reprint Interaction of arginine-rich peptides with membrane-associated proteoglycans is crucial for induction of actin organization and macropinocytosis
    Ikuhiko Nakase
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 46:492-501. 2007
    ..These data strongly suggest the possibility that the interaction of the arginine-rich peptides with the membrane-associated proteoglycans quickly activates the intracellular signals and induces actin organization and macropinocytotis...
  38. ncbi request reprint PDX-1 protein is internalized by lipid raft-dependent macropinocytosis
    Hirofumi Noguchi
    Department of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Sakyo ku, Japan
    Cell Transplant 14:637-45. 2005
    ..Taken together, these observations provide the mechanism of PDX-1 protein transduction and suggest that the protein transduction system could work for experimental and therapeutic strategies...
  39. ncbi request reprint Rev-derived peptides inhibit HIV-1 replication by antagonism of Rev and a co-receptor, CXCR4
    Kazuki Shimane
    Laboratory of Virus Control, Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Int J Biochem Cell Biol 42:1482-8. 2010
    ..Interestingly, similar motif of human lymphotropic virus type I Rex (Rex(1-21)) also exerted moderate anti-HIV-1 activity. These results indicate that arginine-rich peptide, Rev(34-50)-A(4)C exerts dual antagonism against CXCR4 and Rev...
  40. pmc Metal-assisted channel stabilization: disposition of a single histidine on the N-terminus of alamethicin yields channels with extraordinarily long lifetimes
    Daisuke Noshiro
    Institute for Chemical Research, Kyoto University, Kyoto, Japan
    Biophys J 98:1801-8. 2010
    ..Channel stability was greatly diminished by acetylation of the N-terminal amino group, indicating that the N-terminal amino group also plays an important role in metal coordination...
  41. ncbi request reprint [Artificial transcription factors based on multi-zinc finger motifs]
    Tatsuya Morisaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan
    Yakugaku Zasshi 130:45-8. 2010
    ..There is much interest in creating gene regulators, and the artificial transcription factors based on multi-zinc finger motifs could be a superior scaffold...
  42. doi request reprint Rapid transcriptional activity in vivo and slow DNA binding in vitro by an artificial multi-zinc finger protein
    Tatsuya Morisaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochemistry 47:10171-7. 2008
    ..There is much interest in creating therapeutic molecules, and these findings suggest the significant potential of multi-zinc finger proteins as a tool for an artificial gene regulator...
  43. doi request reprint Positive and negative cooperativity of modularly assembled zinc fingers
    Miki Imanishi
    Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan
    Biochem Biophys Res Commun 387:440-3. 2009
    ..Our results indicate that not only a positive cooperativity but also a context-dependent reduction in the DNA binding activity can be induced by assembling zinc finger modules...
  44. doi request reprint Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Sp1
    Miki Imanishi
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 400:625-30. 2010
    ..We propose a new molecular mechanism for zinc finger-zinc finger interactions that should contribute to our understanding of the bio-functional role of the interaction between GATA-1 and Sp1...
  45. ncbi request reprint Control of peptide structure and recognition by Fe(III)-induced helix destabilization
    Shiroh Futaki
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Am Chem Soc 126:15762-9. 2004
    ..Mutual recognition of the transcription factor Jun-derived leucine-zipper peptide segment with the Fos leucine-zipper segment containing Ida residues was also modulated in the presence of Fe(III)...
  46. ncbi request reprint Cell permeable peptide of JNK inhibitor prevents islet apoptosis immediately after isolation and improves islet graft function
    Hirofumi Noguchi
    Department of Transplantation and Immunology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Am J Transplant 5:1848-55. 2005
    ..These findings suggest that peptide drugs could be useful for the prevention of the impairment of islet cells and lead to improvement in the outcomes for pancreatic islet transplantation...
  47. ncbi request reprint Generation of reactive oxygen species and activation of NF-kappaB by non-Abeta component of Alzheimer's disease amyloid
    Seigo Tanaka
    Laboratory of Molecular Clinical Chemistry, Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan
    J Neurochem 82:305-15. 2002
    ..These results suggest that NAC fibrils increase mitochondrial ROS generation and activate NF-kappaB, thereby causing a differential change in gene expression between neurons and astrocytes in the AD brain...
  48. ncbi request reprint The structure of the promoter region for rat inducible nitric oxide synthase gene
    M Niwa
    Institute for Medicinal Resources, The University of Tokushima, Sho machi, Japan
    Life Sci 61:PL 45-9. 1997
    ..The sequences were highly homologous with the mouse iNOS gene. The regulation of the iNOS gene may be distinguished between rodent and mammalian...
  49. doi request reprint Novel system to achieve one-pot modification of cargo molecules with oligoarginine vectors for intracellular delivery
    Kentaro Takayama
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Bioconjug Chem 20:249-57. 2009
    ....
  50. ncbi request reprint Site-specific DNA cleavage by artificial zinc finger-type nuclease with cerium-binding peptide
    Takako Nakatsukasa
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 330:247-52. 2005
    ..The present result provides useful information for expanding the design strategy for artificial nucleases...
  51. ncbi request reprint Anionic fullerenes, calixarenes, coronenes, and pyrenes as activators of oligo/polyarginines in model membranes and live cells
    Florent Perret
    Department of Organic Chemistry, University of Geneva, Geneva, Switzerland, Institute for Chemical Research, Kyoto University and PRESTO, JST, Kyoto, Japan
    J Am Chem Soc 127:1114-5. 2005
    ..Applied to HeLa cells, pyrenebutyrate is shown to significantly increase the uptake of a fluorescently labeled octaarginine in a concentration-dependent manner...
  52. doi request reprint Expressed protein ligation for the preparation of fusion proteins with cell penetrating peptides for endotoxin removal and intracellular delivery
    Hao Hsin Yu
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochim Biophys Acta 1798:2249-57. 2010
    ..Fusion proteins of EGFP with R12 and the d-isomer of R12 prepared by EPL showed similar levels of cellular uptake compared to the fusion protein directly expressed in Escherichiacoli...
  53. ncbi request reprint Topological stability and self-association of a completely hydrophobic model transmembrane helix in lipid bilayers
    Yoshiaki Yano
    Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Biochemistry 41:3073-80. 2002
    ..Electron spin resonance spectra of 1-palmitoyl-2-stearoyl-(14-doxyl)-sn-glycero-3-phosphocholine demonstrated the presence of a motionally restricted component at lower temperatures...
  54. ncbi request reprint Benzoquinones inhibit the expression of inducible nitric oxide synthase gene
    M Niwa
    Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan
    Biochem Biophys Res Commun 239:367-71. 1997
    ..Benzoquinones may be a useful candidate for the development of a drug to treat disease due to iNOS gene over-expression...
  55. ncbi request reprint NMR investigation of the electrostatic effect in binding of a neuropeptide, achatin-I, to phosphatidylcholine bilayers
    Tomohiro Kimura
    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611 0011, Japan
    J Phys Chem B 111:3831-8. 2007
    ..Hence, the electrostatic effect of the side chain beta-CO2(-) in Asp4 floats achatin-I on the PC membrane surface, and the binding equilibrium is sensitively controlled by the ionization state of the N-terminal NH3(+)...
  56. ncbi request reprint Evaluation of two sperm antigens, rSMP-B and YWK-II, as targets for immunocontraception
    M Takikawa
    Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Japan
    Zygote 9:145-51. 2001
    ..A second sperm protein, YWK-II, is effective as an antifertility immunogen in experimental animals...
  57. ncbi request reprint beta-Microseminoprotein/prostatic secretory protein is a member of immunoglobulin binding factor family
    M Kamada
    Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, 3 18 15 Kuramoto cho, Tokushima 770 8503, Japan
    Biochim Biophys Acta 1388:101-10. 1998
    ..The present data clearly show that IgBF is a family composed of at least three isoforms. One of the members is beta-MSP/PSP94. This family should be designated as IgBF...
  58. ncbi request reprint Octaarginine- and octalysine-modified nanoparticles have different modes of endosomal escape
    Ayman El-Sayed
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita ku, Sapporo, Hokkaido 060 8012, Japan
    J Biol Chem 283:23450-61. 2008
    ..Therefore, it was concluded that R8 on the liposome surface stimulates efficient escape from endocytic vesicles via a fusion mechanism that works at both neutral and acidic pH; in contrast, K8 mediates escape mainly at neutral pH...
  59. doi request reprint An artificial virus-like nano carrier system: enhanced endosomal escape of nanoparticles via synergistic action of pH-sensitive fusogenic peptide derivatives
    Kentaro Sasaki
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, 060 0812, Japan
    Anal Bioanal Chem 391:2717-27. 2008
    ..We concluded that topological control was responsible for the synergistic effect of GALA derivatives introduced on Tf-PEG-L...
  60. ncbi request reprint Transferrin-modified liposomes equipped with a pH-sensitive fusogenic peptide: an artificial viral-like delivery system
    Tomoyuki Kakudo
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Biochemistry 43:5618-28. 2004
    ..With GALA present on the surface, intracellular trafficking of liposomes after receptor-mediated endocytosis could be successfully controlled...
  61. ncbi request reprint A multifunctional envelope-type nano device for novel gene delivery of siRNA plasmids
    Rumiko Moriguchi
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Int J Pharm 301:277-85. 2005
    ..These results demonstrate that MEND(Prot) is a promising gene delivery system for siRNA expression plasmids with less heterogeneity associated with the transfection...
  62. ncbi request reprint Direct observation of anion-mediated translocation of fluorescent oligoarginine carriers into and across bulk liquid and anionic bilayer membranes
    Naomi Sakai
    Department of Organic Chemistry, University of Geneva, 30, Quai Ernest Ansermet, 1211 Geneva 4, Switzerland
    Chembiochem 6:114-22. 2005
    ....
  63. ncbi request reprint Development of a non-viral multifunctional envelope-type nano device by a novel lipid film hydration method
    Kentaro Kogure
    CREST, Japan Science and Technology Agency JST, Japan
    J Control Release 98:317-23. 2004
    ..The MEND developed in this study represents a promising non-viral gene delivery system...
  64. ncbi request reprint pH-sensitive liposomes in nuclear targeting of macromolecules
    Rieko Tachibana
    Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi 1 78 1, 770 8505 Tokushima, Japan
    Methods Enzymol 372:349-61. 2003
  65. ncbi request reprint Synthetic copoly(Lys/Phe) and poly(Lys) translocate through lipid bilayer membranes
    Akira Shibata
    Faculty of Pharmaceutical Sciences, Tokushima University, Shomachi, Tokushima 770 8505, Japan
    Biochim Biophys Acta 1616:147-55. 2003
    ..We presented the first direct observation that the positively charged polypeptides, copoly(Lys/Phe) (MW: 41,500) and poly(Lys) (MW: 23,400), could translocate across the lipid bilayer membrane...
  66. ncbi request reprint Can nuclear localization signals enhance nuclear localization of plasmid DNA?
    Takeshi Nagasaki
    Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, SORST JST, Japan
    Bioconjug Chem 14:282-6. 2003
    ..Our findings suggest that stimulation of plasmid nuclear localization cannot be achieved merely by changing spacer length or chemically modifying plasmid DNA-NLS peptide conjugates. An additional mechanism must be involved...
  67. doi request reprint Octaarginine-modified liposomes: enhanced cellular uptake and controlled intracellular trafficking
    Ikramy A Khalil
    Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita ku, Sapporo, Hokkaido, Japan
    Int J Pharm 354:39-48. 2008
    ..In addition, the use of R8-modified liposomes and nano-particles in gene delivery is discussed...
  68. ncbi request reprint Membrane permeable peptide vectors: chemistry and functional design for the therapeutic applications
    Shiroh Futaki
    Adv Drug Deliv Rev 60:447. 2008
  69. ncbi request reprint Intracellular traffic and fate of protein transduction domains HIV-1 TAT peptide and octaarginine. Implications for their utilization as drug delivery vectors
    Saly Al-Taei
    Welsh School of Pharmacy, Cardiff University, Cardiff, CF10 3XF, Wales, United Kingdom
    Bioconjug Chem 17:90-100. 2006
    ..Disruption of Golgi and endoplasmic reticulum dynamics was without effect on peptide localization, suggesting that endosomes and lysosomes rather than these organelles are the major acceptor compartments for these molecules...
  70. ncbi request reprint Design, synthesis, and membrane-translocation studies of inositol-based transporters
    Kaustabh K Maiti
    Department of Chemistry, Pohang University of Science and Technology, Pohang 790 784, Korea
    Angew Chem Int Ed Engl 45:2907-12. 2006
  71. ncbi request reprint Evaluation of the nuclear delivery and intra-nuclear transcription of plasmid DNA condensed with micro (mu) and NLS-micro by cytoplasmic and nuclear microinjection: a comparative study with poly-L-lysine
    Hidetaka Akita
    Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan
    J Gene Med 8:198-206. 2006
    ..In an attempt to achieve nuclear delivery, NLS-mu, a novel pDNA condenser, was prepared. This consists of mu, a highly potent polypeptide for condensing the pDNA, and a SV40 T antigen-derived nuclear localization signal (NLS(SV40))...
  72. ncbi request reprint Effects of cell-permeating peptide binding on the distribution of 125I-labeled Fab fragment in rats
    Shouju Kameyama
    Research Planning, Bipha Corporation, Chitose, Hokkaido 066 0051, Japan
    Bioconjug Chem 17:597-602. 2006
    ..These findings provide valuable information for the development of novel antibody pharmaceuticals and therapeutic systems...
  73. ncbi request reprint Cellular uptake and subsequent intracellular trafficking of R8-liposomes introduced at low temperature
    Akitada Iwasa
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060 0812, Japan
    Biochim Biophys Acta 1758:713-20. 2006
    ..In addition, the uptake route is closely related to the subsequent nuclear delivery process; the operation of an endogenous vesicular sorting system is advantageous for the nuclear delivery of pDNA...
  74. ncbi request reprint Effects of Na+/H+ exchanger inhibitors on subcellular localisation of endocytic organelles and intracellular dynamics of protein transduction domains HIV-TAT peptide and octaarginine
    Marjan Fretz
    Welsh School of Pharmacy, Redwood Building, Cardiff University, Cardiff, CF10 3XF, Wales, UK
    J Control Release 116:247-54. 2006
    ..At levels previously shown to inhibit uptake of HIV-TAT peptide and octaarginine in other cell lines, EIPA was without major effect on uptake of both peptides in KG1a cells...
  75. ncbi request reprint Direct and rapid cytosolic delivery using cell-penetrating peptides mediated by pyrenebutyrate
    Toshihide Takeuchi
    ACS Chem Biol 1:299-303. 2006
    ..Thus, coupling arginine-rich peptides with this hydrophobic anion dramatically improved their ability to translocate cellular membranes, suggesting the great impact of this approach on exploring and controlling cell function...
  76. pmc Temperature-, concentration- and cholesterol-dependent translocation of L- and D-octa-arginine across the plasma and nuclear membrane of CD34+ leukaemia cells
    Marjan M Fretz
    Welsh School of Pharmacy, Cardiff University, Cardiff CF10 3XF, Wales, UK
    Biochem J 403:335-42. 2007
    ..The data argue for two, temperature-dependent, uptake mechanism for these peptides and for the existence of a threshold concentration for endocytic uptake that when exceeded promotes direct translocation across the plasma membrane...
  77. ncbi request reprint High density of octaarginine stimulates macropinocytosis leading to efficient intracellular trafficking for gene expression
    Ikramy A Khalil
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido 060 0812
    J Biol Chem 281:3544-51. 2006
    ..These results show that the density of the R8 peptide on liposomes determines the uptake mechanism and that this is directly linked to intracellular trafficking, resulting in different levels of gene expression...
  78. ncbi request reprint Guanidine-containing molecular transporters: sorbitol-based transporters show high intracellular selectivity toward mitochondria
    Kaustabh K Maiti
    Department of Chemistry, Pohang University of Science and Technology, Pohang 790 784, Korea
    Angew Chem Int Ed Engl 46:5880-4. 2007
  79. ncbi request reprint Mitochondrial delivery of mastoparan with transferrin liposomes equipped with a pH-sensitive fusogenic peptide for selective cancer therapy
    Yuma Yamada
    Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060 0812, Japan
    Int J Pharm 303:1-7. 2005
    ..These results demonstrate the utility of MP in Tf-L with GALA for cancer therapy...
  80. ncbi request reprint Octaarginine-modified multifunctional envelope-type nano device for siRNA
    Yoshio Nakamura
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    J Control Release 119:360-7. 2007
    ..Consequently, R8-MEND can deliver condensed siRNA into cells to produce an efficient and persistent silencing effect with minimum cytotoxicity...
  81. ncbi request reprint New packaging method of mycobacterial cell wall using octaarginine-modified liposomes: enhanced uptake by and immunostimulatory activity of dendritic cells
    Atthachai Homhuan
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo, Hokkaido, Japan
    J Control Release 120:60-9. 2007
    ..We assert that R8-modified liposomes with mycobacterial CW incorporated should have tremendous potential as immune-potentiating agents...
  82. ncbi request reprint Acid wash in determining cellular uptake of Fab/cell-permeating peptide conjugates
    Shouju Kameyama
    Research Planning, Bipha Corporation, Chitose, Hokkaido, Japan
    Biopolymers 88:98-107. 2007
    ....
  83. ncbi request reprint Alpha,alpha-disubstituted glycines bearing a large hydrocarbon ring: peptide self-assembly through hydrophobic recognition
    Tomohiko Ohwada
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Chemistry 10:617-26. 2004
    ..The results of sedimentation equilibrium studies suggested that the peptide assembles into hexamers in the presence of 100 mM NaCl...
  84. ncbi request reprint Significant and prolonged antisense effect of a multifunctional envelope-type nano device encapsulating antisense oligodeoxynucleotide
    Yoshio Nakamura
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060 0812, Japan
    J Pharm Pharmacol 58:431-7. 2006
    ..In conclusion, we successfully developed an ODN-MEND with a high antisense effect using protamine as a DNA condensing as well as a decondensing agent...
  85. ncbi request reprint Distribution of immunoglobulin Fab fragment conjugated with HIV-1 REV peptide following intravenous administration in rats
    Shouju Kameyama
    Research Planning, Bipha Corporation, Chitose, Hokkaido 066 0051, Japan
    Mol Pharm 3:174-80. 2006
    ..With these features, cell-permeating peptides are expected to help the development of new antibody pharmaceuticals...
  86. ncbi request reprint Development of oligoarginine-drug conjugates linked to new peptidic self-cleavable spacers toward effective intestinal absorption
    Yoshio Hayashi
    Department of Medicinal Chemistry, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192 0392, Japan
    Bioorg Med Chem Lett 17:5129-32. 2007
    ..These novel self-cleavable spacers are promising for developments of the OACT system as means to potentially enhance intestinal absorption of parent drugs...
  87. ncbi request reprint Arginine magic with new counterions up the sleeve
    Masamichi Nishihara
    Department of Organic Chemistry, University of Geneva, Geneva, Switzerland
    Org Biomol Chem 3:1659-69. 2005
    ....
  88. ncbi request reprint Pharmacokinetic analysis of the tissue distribution of octaarginine modified liposomes in mice
    Diky Mudhakir
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Drug Metab Pharmacokinet 20:275-81. 2005
    ..Therefore, it is suggested that topology control of R8 is important to exhibit the PTD function...