Kiyokazu Agata

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. ncbi request reprint Two different evolutionary origins of stem cell systems and their molecular basis
    Kiyokazu Agata
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Semin Cell Dev Biol 17:503-9. 2006
  2. ncbi request reprint Unifying principles of regeneration I: Epimorphosis versus morphallaxis
    Kiyokazu Agata
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Dev Growth Differ 49:73-8. 2007
  3. pmc Intra-genomic GC heterogeneity in sauropsids: evolutionary insights from cDNA mapping and GC(3) profiling in snake
    Kazumi Matsubara
    Department of Information and Biological Sciences, Graduate School of Natural Sciences, Nagoya City University, 1 Yamanohata, Mizuho cho, Mizuho ku, Nagoya, Aichi 467 8501, Japan
    BMC Genomics 13:604. 2012
  4. doi request reprint Survey of the differences between regenerative and non-regenerative animals
    Kiyokazu Agata
    Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Dev Growth Differ 54:143-52. 2012
  5. pmc The innate immune repertoire in cnidaria--ancestral complexity and stochastic gene loss
    David J Miller
    ARC Centre of Excellence in Coral Reef Studies and Comparative Genomics Centre, James Cook University, Townsville, Queensland, Australia
    Genome Biol 8:R59. 2007
  6. ncbi request reprint [RNA world in stem cell systems]
    Kiyokazu Agata
    Tanpakushitsu Kakusan Koso 51:2562-7. 2006
  7. pmc Brain regeneration from pluripotent stem cells in planarian
    Kiyokazu Agata
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Philos Trans R Soc Lond B Biol Sci 363:2071-8. 2008
  8. doi request reprint The planarian P2X homolog in the regulation of asexual reproduction
    Toshihide Sakurai
    Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan
    Int J Dev Biol 56:173-82. 2012
  9. doi request reprint Comprehensive gene expression analyses in pluripotent stem cells of a planarian, Dugesia japonica
    Norito Shibata
    Global COE Program Evolution and Biodiversity, Graduate School of Science, Kyoto University, Japan
    Int J Dev Biol 56:93-102. 2012
  10. doi request reprint Regeneration in an evolutionarily primitive brain--the planarian Dugesia japonica model
    Yoshihiko Umesono
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto, 606 8502, Japan
    Eur J Neurosci 34:863-9. 2011

Collaborators

Detail Information

Publications75

  1. ncbi request reprint Two different evolutionary origins of stem cell systems and their molecular basis
    Kiyokazu Agata
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Semin Cell Dev Biol 17:503-9. 2006
    ..Here, we review the evidence for the molecular basis for the evolutionary origin of these two different stem cell systems...
  2. ncbi request reprint Unifying principles of regeneration I: Epimorphosis versus morphallaxis
    Kiyokazu Agata
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Dev Growth Differ 49:73-8. 2007
    ....
  3. pmc Intra-genomic GC heterogeneity in sauropsids: evolutionary insights from cDNA mapping and GC(3) profiling in snake
    Kazumi Matsubara
    Department of Information and Biological Sciences, Graduate School of Natural Sciences, Nagoya City University, 1 Yamanohata, Mizuho cho, Mizuho ku, Nagoya, Aichi 467 8501, Japan
    BMC Genomics 13:604. 2012
    ....
  4. doi request reprint Survey of the differences between regenerative and non-regenerative animals
    Kiyokazu Agata
    Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Dev Growth Differ 54:143-52. 2012
    ....
  5. pmc The innate immune repertoire in cnidaria--ancestral complexity and stochastic gene loss
    David J Miller
    ARC Centre of Excellence in Coral Reef Studies and Comparative Genomics Centre, James Cook University, Townsville, Queensland, Australia
    Genome Biol 8:R59. 2007
    ..Recently, whole genome sequences became available for two cnidarians, Hydra magnipapillata and Nematostella vectensis, and large expressed sequence tag (EST) datasets are available for these and for the coral Acropora millepora...
  6. ncbi request reprint [RNA world in stem cell systems]
    Kiyokazu Agata
    Tanpakushitsu Kakusan Koso 51:2562-7. 2006
  7. pmc Brain regeneration from pluripotent stem cells in planarian
    Kiyokazu Agata
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Philos Trans R Soc Lond B Biol Sci 363:2071-8. 2008
    ..Here we describe the molecular action of the nou-darake gene and propose a new model to explain brain regeneration and restriction in the head region of the planarians...
  8. doi request reprint The planarian P2X homolog in the regulation of asexual reproduction
    Toshihide Sakurai
    Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan
    Int J Dev Biol 56:173-82. 2012
    ..We also found that DjP2X-A is required for normal proliferation in starved animals. We propose that DjP2X-A modulates stem cell proliferation in response to the nutritional condition...
  9. doi request reprint Comprehensive gene expression analyses in pluripotent stem cells of a planarian, Dugesia japonica
    Norito Shibata
    Global COE Program Evolution and Biodiversity, Graduate School of Science, Kyoto University, Japan
    Int J Dev Biol 56:93-102. 2012
    ..Thus, using multidimensional gene expression analyses, we were able to obtain a valuable data set of neoblast-related genes and their expression patterns...
  10. doi request reprint Regeneration in an evolutionarily primitive brain--the planarian Dugesia japonica model
    Yoshihiko Umesono
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto, 606 8502, Japan
    Eur J Neurosci 34:863-9. 2011
    ..Our findings suggest the possible value of in vivo planarian studies for guiding regenerative medicine to treat neurodegenerative diseases via interlinking stem cell biology and regeneration biology...
  11. doi request reprint Piwi expression in archeocytes and choanocytes in demosponges: insights into the stem cell system in demosponges
    Noriko Funayama
    Laboratory of Molecular Developmental Biology, Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Evol Dev 12:275-87. 2010
    ..We discuss the possibility that choanocytes might represent the ancestral stem cells, whereas archeocytes might represent stem cells that further evolved in ancestral multicellular organisms...
  12. doi request reprint ERK signaling controls blastema cell differentiation during planarian regeneration
    Junichi Tasaki
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606 8502, Japan
    Development 138:2417-27. 2011
    ....
  13. doi request reprint Cellular and molecular dissection of pluripotent adult somatic stem cells in planarians
    Norito Shibata
    Global COE Program, Division of Biological Science, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Dev Growth Differ 52:27-41. 2010
    ..In this review, we introduce physiological and molecular features of the neoblast, and discuss how germline genes regulate planarian neoblasts and what differences exist between neoblasts and germline cells...
  14. doi request reprint Regeneration of dopaminergic neurons after 6-hydroxydopamine-induced lesion in planarian brain
    Kaneyasu Nishimura
    Department of Biophysics, Graduate School of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo ku, Kyoto, Japan
    J Neurochem 119:1217-31. 2011
    ..These new findings obtained from planarian regeneration provide hints about how to conduct cell-transplantation therapy for future regenerative medicine...
  15. doi request reprint Role of c-Jun N-terminal kinase activation in blastema formation during planarian regeneration
    Junichi Tasaki
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto 606 8502, Japan
    Dev Growth Differ 53:389-400. 2011
    ..These findings suggest that JNK signaling plays a crucial role in feeding into the blastema neoblasts for differentiation by regulating the G2/M transition in the cell cycle during planarian regeneration...
  16. doi request reprint Different requirements for conserved post-transcriptional regulators in planarian regeneration and stem cell maintenance
    Labib Rouhana
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Dev Biol 341:429-43. 2010
    ..We propose that planarian neoblasts maintain pluripotency by employing mechanisms of post-transcriptional regulation exhibited in germ cells and early development of most metazoans...
  17. pmc Planarian Hedgehog/Patched establishes anterior-posterior polarity by regulating Wnt signaling
    Shigenobu Yazawa
    Department of Biophysics, Division of Biological Sciences, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 106:22329-34. 2009
    ..We therefore propose that Hh signals direct head or tail regeneration according to the AP polarity, which is established by Hh signaling activity along the body's preexisting nervous system...
  18. ncbi request reprint Comparison of molecular and cellular events during lower jaw regeneration of newt (Cynops pyrrhogaster) and West African clawed frog (Xenopus tropicalis)
    Hiroshi Kurosaka
    Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan
    Dev Dyn 237:354-65. 2008
    ..These results suggest that the difference of the jaw-regenerating abilities between newts and frogs depends on the expression of MHC mRNA at the tip of the amputated jaw and the contribution of Pax7-positive cells...
  19. pmc Comparative transcriptome analysis between planarian Dugesia japonica and other platyhelminth species
    Osamu Nishimura
    Department of Biophysics and Global COE Program, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto, Japan
    BMC Genomics 13:289. 2012
    ..Here we conducted large-scale EST analysis of the head region of the planarian Dugesia japonica to construct a database of the head-region transcriptome, and then performed comparative analyses among related species...
  20. doi request reprint Expression and functional analysis of musashi-like genes in planarian CNS regeneration
    Sayaka Higuchi
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Mech Dev 125:631-45. 2008
    ..RNAi experiments suggested that these planarian musashi family genes might be involved in neural cell differentiation after neural cell-fate commitment...
  21. doi request reprint Planarians maintain a constant ratio of different cell types during changes in body size by using the stem cell system
    Hiroyuki Takeda
    Department of Biophysics, Graduate School of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo ku, Kyoto 606 8502, Japan
    Zoolog Sci 26:805-13. 2009
    ....
  22. doi request reprint Evolution and regeneration of the planarian central nervous system
    Yoshihiko Umesono
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Dev Growth Differ 51:185-95. 2009
    ..Our recent findings have provided mechanistic insights into stem cell biology and also evolutionary biology...
  23. doi request reprint Inhibition of MEK and GSK3 supports ES cell-like domed colony formation from avian and reptile embryos
    Shota Nakanoh
    Department of Biophysics, Graduate School of Science, Kyoto University Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Zoolog Sci 30:543-52. 2013
    ..However, gene expression analysis indicated the possibility that mammalian pluripotency transcription factors function differently in non-mammalian amniotes. ..
  24. doi request reprint Lens regenerates by means of similar processes and timeline in adults and larvae of the newt Cynops pyrrhogaster
    Takeshi Inoue
    Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan
    Dev Dyn 241:1575-83. 2012
    ..pyrrhogaster at the cellular level using molecular markers in the present study, we found that lens regeneration during the larval stage proceeded at similar speed and by means of similar steps to those in adults...
  25. pmc Genome features of "Dark-fly", a Drosophila line reared long-term in a dark environment
    Minako Izutsu
    Laboratory for Biodiversity, Global COE Program, Graduate School of Science, Kyoto University, Kyoto, Japan
    PLoS ONE 7:e33288. 2012
    ..Our results revealed unique features of the Dark-fly genome and provided a list of potential candidate genes involved in environmental adaptation...
  26. doi request reprint The active stem cell specific expression of sponge Musashi homolog EflMsiA suggests its involvement in maintaining the stem cell state
    Kazuko Okamoto
    Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
    Mech Dev 129:24-37. 2012
    ..Furthermore, these findings shed light on the mode of cell division of archeocytes and suggest that archeocytes divide symmetrically for both self-renewal and differentiation...
  27. doi request reprint Optic chiasm formation in planarian I: Cooperative netrin- and robo-mediated signals are required for the early stage of optic chiasm formation
    Hiroshi Yamamoto
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Dev Growth Differ 53:300-11. 2011
    ..These results suggest that two different collaborative or combinatory signals may be required for midline crossing at the early stage of chiasm formation during eye and brain regeneration...
  28. doi request reprint Toward understanding the morphogenesis of siliceous spicules in freshwater sponge: differential mRNA expression of spicule-type-specific silicatein genes in Ephydatia fluviatilis
    Kurato Mohri
    Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku Kyoto, Japan
    Dev Dyn 237:3024-39. 2008
    ....
  29. doi request reprint Bidirectional promoters are the major source of gene activation-associated non-coding RNAs in mammals
    Masahiro Uesaka
    Department of Biophysics and Global COE Program, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    BMC Genomics 15:35. 2014
    ..To examine the directional properties of transcription at the whole-genome level, we performed directional RNA-seq analysis of mouse and chimpanzee tissue samples...
  30. ncbi request reprint Expression patterns of Xenopus FGF receptor-like 1/nou-darake in early Xenopus development resemble those of planarian nou-darake and Xenopus FGF8
    Shuichi Hayashi
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan
    Dev Dyn 230:700-7. 2004
    ..In many of these regions, XFGFRL1/Xndk was coexpressed with XFGF8, indicating that XFGFRL1/Xndk is a member of the XFGF8 synexpression group, which includes sprouty, sef, and isthmin...
  31. ncbi request reprint Inhibitory effects of antiparkinsonian drugs and caspase inhibitors in a parkinsonian flatworm model
    Yoshihisa Kitamura
    Department of Neurobiology, Kyoto Pharmaceutical University, Kyoto, Japan
    J Pharmacol Sci 92:137-42. 2003
    ..These results suggest that the degeneration of planarian dopaminergic system induced by rotenone or MPTP may be mediated through caspase-like activation...
  32. doi request reprint Modelling the global distribution of fungal species: new insights into microbial cosmopolitanism
    Hirotoshi Sato
    Graduate School of Global Environmental Studies, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    Mol Ecol 21:5599-612. 2012
    ..We hypothesize that the degree of host/habitat specificity and the degree of isolation of potentially suitable habitats determine microbial biogeographic patterns...
  33. doi request reprint Staging of regeneration process of an arm of the feather star Oxycomanthus japonicus focusing on the oral-aboral boundary
    Tomoko F Shibata
    Misaki Marine Biological Station, Graduate School of Science, The University of Tokyo, Kanagawa, Japan
    Dev Dyn 239:2947-61. 2010
    ..The mode of morphogenesis of pinnules in arm regeneration suggests that the oral region functions as the primary patterning tissue for pinnules...
  34. ncbi request reprint [Molecular mechanism of brain regeneration and reconstruction of dopaminergic neural network in planarians]
    Kaneyasu Nishimura
    Department of Neurobiology, Kyoto Pharmaceutical University, 5 Nakauchi cho, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Brain Nerve 60:307-17. 2008
    ....
  35. ncbi request reprint [Nuclear reorganization during differentiation of the newt]
    Nobuyasu Maki
    Tanpakushitsu Kakusan Koso 51:2240-4. 2006
  36. ncbi request reprint Characterization and categorization of fluorescence activated cell sorted planarian stem cells by ultrastructural analysis
    Sayaka Higuchi
    RIKEN Center for Developmental Biology, Faculty of Science, Kobe University, Kobe, Japan
    Dev Growth Differ 49:571-81. 2007
    ..Interestingly, they were concentrated in the X2 fraction, containing G0/G1 phase cells. These results suggest that planarian stem cells are not homogeneous, but may consist of heterogeneous populations, like mammalian stem cells...
  37. ncbi request reprint Detection of apoptosis during planarian regeneration by the expression of apoptosis-related genes and TUNEL assay
    Jung Shan Hwang
    Center for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Gene 333:15-25. 2004
    ..We hypothesize that the presence of apoptosis in planarians may have a role in controlling cell numbers, eliminating unnecessary tissues or cells and remodeling the old tissues of regenerating body parts...
  38. ncbi request reprint [RNA-binding proteins in stem cell system of planarians]
    Maki Kashikawa
    Tanpakushitsu Kakusan Koso 48:438-43. 2003
  39. ncbi request reprint Identification and origin of the germline stem cells as revealed by the expression of nanos-related gene in planarians
    Kimihiro Sato
    Laboratory of Regeneration Biology, Graduate School of Life Science, University of Hyogo, 3 2 1 Koto, Kamigori, Akou gun, Hyogo 678 1297, Japan
    Dev Growth Differ 48:615-28. 2006
    ..However, Djnos-positive cells appeared in the newly formed trunk region from the head piece, suggesting that somatic stem cells can convert to germline stem cells...
  40. ncbi request reprint [Molecular approach to planarian stem cell system]
    Kiyokazu Agata
    Tanpakushitsu Kakusan Koso 50:706-10. 2005
  41. ncbi request reprint Planarian fibroblast growth factor receptor homologs expressed in stem cells and cephalic ganglions
    Kazuya Ogawa
    Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Garden City, Akou, Hyogo 678 1297, Japan
    Dev Growth Differ 44:191-204. 2002
    ..These results suggest that the DjFGFR1 protein may be involved in the signal systems controlling such aspects of planarian regeneration as differentiation/growth/migration of stem cells...
  42. ncbi request reprint Regeneration and gene regulation in planarians
    Kiyokazu Agata
    Evolutionary Regeneration Group, Center for Developmental Biology, RIKEN Kobe, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Curr Opin Genet Dev 13:492-6. 2003
    ..In particular, a gene knockdown method by RNA interference enabled great progress in identifying genes involved in regeneration and stem cell regulation...
  43. ncbi request reprint Rapid accumulation of nucleostemin in nucleolus during newt regeneration
    Nobuyasu Maki
    Center for Developmental Biology, RIKEN Kobe, Chuo Ku, Kobe, Japan
    Dev Dyn 236:941-50. 2007
    ..These findings suggest that nucleostemin plays a role in the dedifferentiation of newt cells and can provide crucial clues for addressing the molecular events at early steps of cellular dedifferentiation in newts...
  44. ncbi request reprint Clathrin-mediated endocytic signals are required for the regeneration of, as well as homeostasis in, the planarian CNS
    Takeshi Inoue
    Group for Evolutionary Regeneration Biology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Kobe, Japan
    Development 134:1679-89. 2007
    ..Thus, clathrin-mediated endocytic signals may be required for not only maintenance of neurons after synaptic formation, but also axonal extension at the early stage of neural differentiation...
  45. ncbi request reprint DjCBC-1, a conserved DEAD box RNA helicase of the RCK/p54/Me31B family, is a component of RNA-protein complexes in planarian stem cells and neurons
    Maki Yoshida-Kashikawa
    RIKEN Center for Developmental Biology, Hyogo, Japan
    Dev Dyn 236:3436-50. 2007
    ..These observations suggest that common molecular components of RNP complexes may be involved in the regulation of somatic and germline stem cells, and neurons in planarians...
  46. pmc Origin and evolutionary process of the CNS elucidated by comparative genomics analysis of planarian ESTs
    Katsuhiko Mineta
    Center for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Proc Natl Acad Sci U S A 100:7666-71. 2003
    ..This implies that the origin of nervous system-related genes greatly predated the emergence of the nervous system, and that these genes might have been recruited toward the nervous system...
  47. ncbi request reprint Search for the evolutionary origin of a brain: planarian brain characterized by microarray
    Masumi Nakazawa
    Center for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, Mishima, Japan
    Mol Biol Evol 20:784-91. 2003
    ..We demonstrate the complex cytoarchitecture of the planarian brain, despite its simple superficiality of the morphology...
  48. ncbi request reprint The Zoological Society Prize. Molecular and cellular approaches to planarian regeneration
    Kiyokazu Agata
    Center for Developmental Biology, RIKEN, Kobe, Japan
    Zoolog Sci 19:1391-2. 2002
  49. ncbi request reprint The expression of planarian brain factor homologs, DjFoxG and DjFoxD
    Satoshi Koinuma
    Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute of Technology, 3 2 1 Kouto, Kamigori cho, Ako gun, Hyogo 678 1297, Japan
    Gene Expr Patterns 3:21-7. 2003
    ..Strong expression was detected in the mid-anterior blastema. Thus, FoxG and FoxD homologs do exist in planarians, but are regulated differently than those in vertebrates...
  50. ncbi request reprint Mediolateral intercalation in planarians revealed by grafting experiments
    Yumi Saito
    Laboratory for Evolutionary Regeneration Biology, Center for Developmental Biology, RIKEN Kobe, Hyogo, Japan
    Dev Dyn 226:334-40. 2003
    ..quot; When the midline tissues were implanted into a lateral region, they induced a complete ectopic head, demonstrating that M-L intercalation may be sufficient to establish the L-R axis in planarians...
  51. ncbi request reprint Cultivation and characterization of planarian neuronal cells isolated by fluorescence activated cell sorting (FACS)
    Maki Asami
    Department of Biofunctional Chemistry, Faculty of Biomolecular Science, Okayama University Graduate School of Natural Science and Technology, Okayama, Japan
    Zoolog Sci 19:1257-65. 2002
    ..When they were cultured in vitro, they showed an ability to extend neurites on several types of extracellular matrices (ECMs), and, depending on the ECM type used, presented a high level of plasticity in morphology and gene expression...
  52. ncbi request reprint FGFR-related gene nou-darake restricts brain tissues to the head region of planarians
    Francesc Cebria
    Group for Evolutionary Regeneration Biology, Center for Developmental Biology RIKEN Kobe, 2 2 3 Minatojima minamimachi, Kobe 650 0047, Japan
    Nature 419:620-4. 2002
    ..Additionally, ndk inhibits FGF signalling in Xenopus embryos. The data suggest that ndk may modulate FGF signalling in stem cells to restrict brain tissues to the head region of planarians...
  53. ncbi request reprint Induction of a noggin-like gene by ectopic DV interaction during planarian regeneration
    Kazuya Ogawa
    Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo, Japan
    Dev Biol 250:59-70. 2002
    ..These results suggest that ectopic DV interaction may induce expression of Djnlg in the positional cue-producing cells, and that it might be involved in stimulation of blastema formation as well as DV patterning of the body...
  54. ncbi request reprint The body margin of the planarian Dugesia japonica: characterization by the expression of an intermediate filament gene
    Akira Tazaki
    Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Garden City, Akou gun, Hyogo 678 1297, Japan
    Dev Genes Evol 212:365-73. 2002
    ..The specific expression pattern of the DjIFb gene enabled us to monitor how the body margin forms during planarian regeneration...
  55. ncbi request reprint The expression of neural-specific genes reveals the structural and molecular complexity of the planarian central nervous system
    Francesc Cebria
    Evolutionary Regeneration Group, Center for Developmental Biology RIKEN Kobe, 2 2 3 Minatojima minamimachi, Chuo Ku, Japan
    Mech Dev 116:199-204. 2002
    ..This high level of regionalization in the planarian brain contrasts with its apparent simplicity at the morphological level...
  56. ncbi request reprint Expression of crystallin genes in embryonic and regenerating newt lenses
    Nobuhiko Mizuno
    Kondoh Differentiation Signaling Project ERATO, Institute for Molecular and Cellular Biology, Osaka University, 1 3 Yamadaoka, Suitashi, Japan
    Dev Growth Differ 44:251-6. 2002
    ....
  57. ncbi request reprint Dissecting planarian central nervous system regeneration by the expression of neural-specific genes
    Francesc Cebria
    Evolutionary Regeneration Biology Group, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Dev Growth Differ 44:135-46. 2002
    ..Three stages in planarian CNS regeneration are suggested...
  58. ncbi request reprint Morphological and functional recovery of the planarian photosensing system during head regeneration
    Takeshi Inoue
    Faculty of Biomolecular Science, Okayama University Graduate School of Natural Science and Technology, Japan
    Zoolog Sci 21:275-83. 2004
    ....
  59. ncbi request reprint Planarian cytochrome b(561): conservation of a six transmembrane structure and localization along the central and peripheral nervous system
    Akikazu Asada
    Department of Life Science, Faculty of Science, Himeji Institute of Technology, Kamigoori cho, Akou gun, Hyogo 678 1297, Japan
    J Biochem 131:175-82. 2002
    ..The present results suggest that the usage of ascorbate to supply electron equivalents to neuroendocrine-specific copper-containing monooxygenases is likely to have originated in organisms with a very simple nervous system...
  60. ncbi request reprint Wnt signaling is required for antero-posterior patterning of the planarian brain
    Chiyoko Kobayashi
    RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Dev Biol 306:714-24. 2007
    ..These observations suggest that the nou-darake/FGFR signal may be involved in brain rudiment formation during the early stage of head regeneration, and subsequently the DjwntA signal may function in A-P patterning of the brain rudiment...
  61. ncbi request reprint Establishment of high-resolution FISH mapping system and its application for molecular cytogenetic characterization of chromosomes in newt, Cynops pyrrhogaster (Urodela, Amphibia)
    Takahiro Murakami
    Department of Nutrition and Food Sciences, Ehime Women s College, 421 Ibuki, Uwajima, Ehime, Japan
    Chromosome Res 15:471-84. 2007
    ..pyrrhogaster and European newts; in contrast, there was species specificity of nucleotide sequences for centromere-specific satellite DNAs...
  62. ncbi request reprint Regeneration-dependent conditional gene knockdown (Readyknock) in planarian: demonstration of requirement for Djsnap-25 expression in the brain for negative phototactic behavior
    Tomomi Takano
    Center for Developmental Biology, RIKEN Kobe, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Dev Growth Differ 49:383-94. 2007
    ..Our approach demonstrates that planarians are a powerful model organism to study the molecular basis of the brain as an information-processing center...
  63. ncbi request reprint Reconstruction of dopaminergic neural network and locomotion function in planarian regenerates
    Kaneyasu Nishimura
    Department of Neurobiology, 21st Century COE Program, Kyoto Pharmaceutical University, Kyoto 607 8414, Japan
    Dev Neurobiol 67:1059-78. 2007
    ..In DjTH-knockdown planarians, muscle-mediated locomotion and behavior were significantly attenuated. These results suggest that DA neurons play a key role in the muscle-mediated movement in planarians...
  64. ncbi request reprint The mRNA-like noncoding RNA Gomafu constitutes a novel nuclear domain in a subset of neurons
    Masamitsu Sone
    Nakagawa Initiative Research Unit, RIKEN, 2 1 Hirosawa, Wako 351 0198, Japan
    J Cell Sci 120:2498-506. 2007
    ..We propose that Gomafu RNA represents a novel family of mRNA-like noncoding RNA that constitutes a cell-type-specific component of the nuclear matrix...
  65. ncbi request reprint Identification and distribution of tryptophan hydroxylase (TPH)-positive neurons in the planarian Dugesia japonica
    Kaneyasu Nishimura
    Department of Neurobiology and 21st Century COE Program, Kyoto Pharmaceutical University, Misasagi, Yamashina ku, Kyoto 607 8414, Japan
    Neurosci Res 59:101-6. 2007
    ..These results suggest that DjTPH is required for 5-HT biosynthesis, and DjTPH antibody is a useful marker for serotonergic neurons in planarians...
  66. ncbi request reprint Structure and function of primitive immunoglobulin superfamily neural cell adhesion molecules: a lesson from studies on planarian
    Eri Fusaoka
    Department of Biological Science, Nagoya University Graduate School of Science, Furocho, Chikusa ku, Nagoya 464 8602, Japan
    Genes Cells 11:541-55. 2006
    ..Functional analyses using RNA interference revealed that DjCAM is partly involved in axon formation, and that DjDSCAM plays crucial roles in neuronal cell migration, axon outgrowth, fasciculation and projection...
  67. ncbi request reprint Isolation of planarian X-ray-sensitive stem cells by fluorescence-activated cell sorting
    Tetsutaro Hayashi
    RIKEN Center for Developmental Biology, Minatojima Minamimachi, Chuo Ku, Kobe, Japan
    Dev Growth Differ 48:371-80. 2006
    ..This method of isolating X-ray-sensitive cells using FACS may provide a key tool for advancing our understanding of the stem cell system in planarians...
  68. ncbi request reprint Evolutionary embryology resurrected in Japan with a new molecular basis: Nori Satoh and the history of ascidian studies originating in Kyoto during the 20th century
    Shigeru Kuratani
    Laboratory for Evolutionary Morphology, Center for Developmental Biology, RIKEN, Kobe, Japan
    Int J Dev Biol 50:451-4. 2006
    ..He is not only known as a founder of molecular biology-based tunicate studies, but also for his world-wide service to education and his prestigious publications in international scientific journals...
  69. ncbi request reprint A wide-range phylogenetic analysis of Zic proteins: implications for correlations between protein structure conservation and body plan complexity
    Jun Aruga
    Laboratory for Comparative Neurogenesis, RIKEN Brain Science Institute, Wako 351 0198, Japan
    Genomics 87:783-92. 2006
    ..The result indicates that the ancestral bilateralian Zic protein had already acquired an entire set of conserved domains, but that this was lost and diverged in the platyhelminthes, nematodes, and urochordates...
  70. ncbi request reprint cDNA-based gene mapping and GC3 profiling in the soft-shelled turtle suggest a chromosomal size-dependent GC bias shared by sauropsids
    Shigehiro Kuraku
    Laboratory for Evolutionary Morphology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, 650 0047, Japan
    Chromosome Res 14:187-202. 2006
    ....
  71. ncbi request reprint Isolation of Ef silicatein and Ef lectin as molecular markers for sclerocytes and cells involved in innate immunity in the freshwater sponge Ephydatia fluviatilis
    Noriko Funayama
    Group for Evolutionary Regeneration Biology, Center for Developmental Biology, RIKEN Kobe, Minatojima Minami, Japan
    Zoolog Sci 22:1113-22. 2005
    ..Therefore, Ef lectin may be specifically expressed in differentiating and/or differentiated cells. We suggest Ef lectin as a marker for cells that assume innate immunity in freshwater sponges...
  72. ncbi request reprint Neural projections in planarian brain revealed by fluorescent dye tracing
    Keiji Okamoto
    Group for Evolutionary Regeneration Biology, Center for Developmental Biology, RIKEN Kobe, Japan
    Zoolog Sci 22:535-46. 2005
    ..Here, we report for the first time on neural connections in the planarian central nervous system after overcoming technical difficulties specific to flatworms...
  73. ncbi request reprint Isolation of the choanocyte in the fresh water sponge, Ephydatia fluviatilis and its lineage marker, Ef annexin
    Noriko Funayama
    Group for Evolutionary Regeneration Biology, Center for Developmental Biology, RIKEN Kobe, 2 2 3 Minatojima minami, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Dev Growth Differ 47:243-53. 2005
    ..This suggests that the single choanocyte chamber essentially originates from a single archeocyte...
  74. ncbi request reprint Crystal structure of the N-terminal RecA-like domain of a DEAD-box RNA helicase, the Dugesia japonica vasa-like gene B protein
    Kazuki Kurimoto
    RIKEN Genomic Sciences Center, Tsurumi, Yokohama 230 0045, Japan
    J Struct Biol 150:58-68. 2005
    ..Therefore, the structural features that we found are characteristic of the PL10-subfamily proteins and might contribute to their biological roles in germ-line development...
  75. ncbi request reprint The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system
    Hideyuki Ishizuka
    Department of Biological Sciences and Informatics, Keio University, Hiyoshi, Yokohama, Japan
    Zoolog Sci 24:31-7. 2007
    ..bio.keio.ac.jp/planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians...