Shinya Nagamatsu

Summary

Affiliation: Kyorin University
Country: Japan

Publications

  1. doi request reprint Insulin exocytosis in Goto-Kakizaki rat beta-cells subjected to long-term glinide or sulfonylurea treatment
    Junko Kawai
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Biochem J 412:93-101. 2008
  2. pmc Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    J Cell Biol 177:695-705. 2007
  3. ncbi request reprint Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules
    S Nagamatsu
    Departments of Biochemistry, Clinical Pathology, Internal Medicine III, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    J Cell Sci 114:219-227. 2001
  4. ncbi request reprint Adenovirus-mediated preproinsulin gene transfer into adipose tissues ameliorates hyperglycemia in obese diabetic KKA(y) mice
    S Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan
    FEBS Lett 509:106-10. 2001
  5. ncbi request reprint Cell biology. IP7 debut in insulin release
    Shinya Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    Science 318:1249-50. 2007
  6. ncbi request reprint Imaging docking and fusion of insulin granules induced by antidiabetes agents: sulfonylurea and glinide drugs preferentially mediate the fusion of newcomer, but not previously docked, insulin granules
    Shinya Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    Diabetes 55:2819-25. 2006
  7. ncbi request reprint Decreased expression of t-SNARE, syntaxin 1, and SNAP-25 in pancreatic beta-cells is involved in impaired insulin secretion from diabetic GK rat islets: restoration of decreased t-SNARE proteins improves impaired insulin secretion
    S Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Diabetes 48:2367-73. 1999
  8. ncbi request reprint alpha-soluble N-ethylmaleimide-sensitive factor attachment protein is expressed in pancreatic beta cells and functions in insulin but not gamma-aminobutyric acid secretion
    S Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611 Japan
    J Biol Chem 274:8053-60. 1999
  9. pmc ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence mi
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Mol Biol Cell 16:3289-300. 2005
  10. ncbi request reprint PPAR-gamma overexpression selectively suppresses insulin secretory capacity in isolated pancreatic islets through induction of UCP-2 protein
    Eisuke Ito
    Third Department of Internal Medicine, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
    Biochem Biophys Res Commun 324:810-4. 2004

Collaborators

Detail Information

Publications77

  1. doi request reprint Insulin exocytosis in Goto-Kakizaki rat beta-cells subjected to long-term glinide or sulfonylurea treatment
    Junko Kawai
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Biochem J 412:93-101. 2008
    ..We conclude that treatment of GK rats with nateglinide and glibenclamide varies in long-term effects on beta-cell functions; nateglinide treatment appears overall to be more beneficial...
  2. pmc Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    J Cell Biol 177:695-705. 2007
    ..We conclude that the first phase is Synt1A dependent but the second phase is not. This indicates that the two phases of insulin exocytosis differ spatially and mechanistically...
  3. ncbi request reprint Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules
    S Nagamatsu
    Departments of Biochemistry, Clinical Pathology, Internal Medicine III, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    J Cell Sci 114:219-227. 2001
    ....
  4. ncbi request reprint Adenovirus-mediated preproinsulin gene transfer into adipose tissues ameliorates hyperglycemia in obese diabetic KKA(y) mice
    S Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan
    FEBS Lett 509:106-10. 2001
    ..This strategy could provide a novel and, most importantly, a simple and convenient gene therapy for obese type 2 diabetes patients...
  5. ncbi request reprint Cell biology. IP7 debut in insulin release
    Shinya Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    Science 318:1249-50. 2007
  6. ncbi request reprint Imaging docking and fusion of insulin granules induced by antidiabetes agents: sulfonylurea and glinide drugs preferentially mediate the fusion of newcomer, but not previously docked, insulin granules
    Shinya Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    Diabetes 55:2819-25. 2006
    ....
  7. ncbi request reprint Decreased expression of t-SNARE, syntaxin 1, and SNAP-25 in pancreatic beta-cells is involved in impaired insulin secretion from diabetic GK rat islets: restoration of decreased t-SNARE proteins improves impaired insulin secretion
    S Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Diabetes 48:2367-73. 1999
    ..2 mmol/l glucose) insulin release was evident between them. We conclude that decreased expression of t-SNAREs in GK rat islets is in part the defect responsible for impaired insulin secretion...
  8. ncbi request reprint alpha-soluble N-ethylmaleimide-sensitive factor attachment protein is expressed in pancreatic beta cells and functions in insulin but not gamma-aminobutyric acid secretion
    S Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611 Japan
    J Biol Chem 274:8053-60. 1999
    ....
  9. pmc ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence mi
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Mol Biol Cell 16:3289-300. 2005
    ..Moreover, attenuation of ELKS expression by small interfering RNA reduced the glucose-evoked insulin release. These data suggest that the CAZ-related protein ELKS functions in insulin exocytosis from pancreatic beta cells...
  10. ncbi request reprint PPAR-gamma overexpression selectively suppresses insulin secretory capacity in isolated pancreatic islets through induction of UCP-2 protein
    Eisuke Ito
    Third Department of Internal Medicine, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
    Biochem Biophys Res Commun 324:810-4. 2004
    ..And it is possible that its site of action can be located in the energy-consuming exocytotic process of insulin secretory granules, and that the reduction of ATP production through increased UCP-2 reduces insulin exocytosis...
  11. ncbi request reprint Site of docking and fusion of insulin secretory granules in live MIN6 beta cells analyzed by TAT-conjugated anti-syntaxin 1 antibody and total internal reflection fluorescence microscopy
    Mica Ohara-Imaizumi
    Department of Biochemistry II, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    J Biol Chem 279:8403-8. 2004
    ....
  12. pmc TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic beta-cells: different behaviour of granule motion between normal and Goto-Kakizaki diabetic rat beta-cells
    Mica Ohara-Imaizumi
    Department of Biochemistry II, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    Biochem J 381:13-8. 2004
    ..Thus our results indicate that the decreased number of docked insulin granules accounts for the impaired insulin release during the first phase of insulin release in diabetic GK rat beta-cells...
  13. ncbi request reprint Gliclazide protects 3T3L1 adipocytes against insulin resistance induced by hydrogen peroxide with restoration of GLUT4 translocation
    Tatsuhiro Shimoyama
    Third Department of Internal Medicine, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Metabolism 55:722-30. 2006
    ....
  14. ncbi request reprint Functions of pancreatic beta cells and adipocytes in bombesin receptor subtype-3-deficient mice
    Yoko Nakamichi
    Department of Biochemistry II, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    Biochem Biophys Res Commun 318:698-703. 2004
    ..We concluded that impaired glucose metabolism observed in BRS-3-deficient mice was mainly caused by impaired GLUT4 translocation in adipocytes...
  15. ncbi request reprint Docking and fusion of insulin secretory granules in SUR1 knock out mouse beta-cells observed by total internal reflection fluorescence microscopy
    Toshiteru Kikuta
    Department of Biochemistry, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
    FEBS Lett 579:1602-6. 2005
    ..fusion with the plasma membrane. Thus, the impaired docking and fusion results in decreased insulin exocytosis from Sur1KO beta-cells...
  16. pmc Glinide, but not sulfonylurea, can evoke insulin exocytosis by repetitive stimulation: imaging analysis of insulin exocytosis by secretagogue-induced repetitive stimulations
    Kyota Aoyagi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Exp Diabetes Res 2009:278762. 2009
    ..Our data suggest that the effect of glinide on insulin secretory mechanisms is similar to that of glucose...
  17. ncbi request reprint Activators of AMP-activated protein kinase enhance GLUT4 translocation and its glucose transport activity in 3T3-L1 adipocytes
    Shinya Yamaguchi
    Third Department of Internal Medicine, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Am J Physiol Endocrinol Metab 289:E643-9. 2005
    ....
  18. doi request reprint JNK- and IkappaB-dependent pathways regulate MCP-1 but not adiponectin release from artificially hypertrophied 3T3-L1 adipocytes preloaded with palmitate in vitro
    Kazuto Takahashi
    Third Department of Internal Medicine, Kyorin University School of Medicine, Tokyo, Japan
    Am J Physiol Endocrinol Metab 294:E898-909. 2008
    ..In contrast, the parallel increase in adiponectin expression appears to be related to the PPARgamma ligand properties of palmitate...
  19. ncbi request reprint PPAR-gamma overexpression suppresses glucose-induced proinsulin biosynthesis and insulin release synergistically with pioglitazone in MIN6 cells
    Yoko Nakamichi
    Department of Biochemistry II, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    Biochem Biophys Res Commun 306:832-6. 2003
    ..Our results demonstrate that PPAR-gamma plays a negative role in pancreatic beta cells...
  20. ncbi request reprint Pioglitazone improves insulin secretory capacity and prevents the loss of beta-cell mass in obese diabetic db/db mice: Possible protection of beta cells from oxidative stress
    Hitoshi Ishida
    Third Department of Internal Medicine, Kyorin University School of Medicine, Tokyo, Japan
    Metabolism 53:488-94. 2004
    ..Treatment with pioglitazone thus protects against beta-cell damage and would be useful for restoration of insulin secretory capacity in obese diabetes individuals...
  21. ncbi request reprint Transduction of MIN6 beta cells with TAT-syntaxin SNARE motif inhibits insulin exocytosis in biphasic insulin release in a distinct mechanism analyzed by evanescent wave microscopy
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    J Biol Chem 277:50805-11. 2002
    ..Thus, syntaxin-mediated SNARE assembly modulates insulin exocytosis in biphasic insulin release in a distinct way...
  22. ncbi request reprint Alphavbeta3 integrin signaling pathway is involved in insulin-like growth factor I-stimulated human extravillous trophoblast cell migration
    Maryam Kabir-Salmani
    Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Endocrinology 144:1620-30. 2003
    ..These findings indicated alpha(v)beta(3) integrin localization in the core of focal adhesions of EVT cells and that alpha(v)beta(3) integrin signaling pathways are activated in IGF-I-mediated migration of these cells...
  23. ncbi request reprint An insulin-related peptide expressed in 3T3L1 adipocytes is localized in GLUT4 vesicles and secreted in response to exogenous insulin, which augments the insulin-stimulated glucose uptake
    Yoko Nakamichi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    J Cell Sci 116:73-9. 2003
    ..Thus, the data support our hypothesis, indicating that we could construct the insulin-regulated insulin release system in adipocytes by introducing the preproinsulin gene...
  24. pmc Monitoring of exocytosis and endocytosis of insulin secretory granules in the pancreatic beta-cell line MIN6 using pH-sensitive green fluorescent protein (pHluorin) and confocal laser microscopy
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Biochem J 363:73-80. 2002
    ..Thus we demonstrate the visualized motion of insulin granule exocytosis/endocytosis using the pH-sensitive marker, pHluorin...
  25. doi request reprint Imaging exocytosis of single insulin secretory granules with TIRF microscopy
    Shinya Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan
    Methods Mol Biol 440:259-68. 2008
    ..In this review, we describe the method of TIRF for visualizing insulin exocytosis in pancreatic beta-cells...
  26. pmc Change in long-spacing collagen in Descemet's membrane of diabetic Goto-Kakizaki rats and its suppression by antidiabetic agents
    Yoshihiro Akimoto
    Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Exp Diabetes Res 2008:818341. 2008
    ..Long-spacing collagen would thus be a useful indicator for studying diabetic changes in the cornea and the effect of antidiabetic agents...
  27. pmc Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan
    PLoS ONE 5:e15553. 2010
    ..Therefore, to determine the role of CDKAL1 in insulin release from β cells, we studied insulin release profiles in CDKAL1 gene knockout (CDKAL1 KO) mice...
  28. doi request reprint Imaging exocytosis of single glucagon-like peptide-1 containing granules in a murine enteroendocrine cell line with total internal reflection fluorescent microscopy
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Biochem Biophys Res Commun 390:16-20. 2009
    ..Closely similar to the insulin granule fusion from pancreatic beta cells, the regulated biphasic exocytosis from two types of granules may be a common mechanism in glucose-evoked hormone release from endocrine cells...
  29. doi request reprint Pattern of rise in subplasma membrane Ca2+ concentration determines type of fusing insulin granules in pancreatic beta cells
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
    Biochem Biophys Res Commun 385:291-5. 2009
    ..These data suggest that the pattern of the [Ca2+](PM) rise directly determines the types of fusing granules...
  30. ncbi request reprint The cytokine interleukin-1beta reduces the docking and fusion of insulin granules in pancreatic beta-cells, preferentially decreasing the first phase of exocytosis
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
    J Biol Chem 279:41271-4. 2004
    ..This suggests that beta-cell exposure to immune mediators during the course of insulitis might be responsible for the loss of first phase insulin release...
  31. pmc Serotonin regulates glucose-stimulated insulin secretion from pancreatic β cells during pregnancy
    Mica Ohara-Imaizumi
    Departments of Biochemistry and Cell Physiology Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Proc Natl Acad Sci U S A 110:19420-5. 2013
    ..Thus, our data indicate that serotonin, acting in a paracrine/autocrine manner through Htr3, lowers the β cell threshold for glucose and plays an essential role in the increased GSIS of pregnancy. ..
  32. doi request reprint Insulin/phosphoinositide 3-kinase pathway accelerates the glucose-induced first-phase insulin secretion through TrpV2 recruitment in pancreatic β-cells
    Kyota Aoyagi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Biochem J 432:375-86. 2010
    ..Thus our results demonstrate that insulin treatment induced the acceleration of the exocytotic response during the glucose-induced first-phase response by the insertion of TrpV2 into the plasma membrane in a PI3K-dependent manner...
  33. ncbi request reprint Insulinotropic action of glutamate is dependent on the inhibition of ATP-sensitive potassium channel activities in MIN 6 beta cells
    Hidenori Katsuta
    Third Department of Internal Medicine, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
    Biochem Biophys Res Commun 311:660-4. 2003
    ..01). These results indicate that the insulinotropic action of glutamate in beta cells could be derived from the inhibition of K(ATP) channel activities, probably due to generation of messengers via intracellular metabolism such as ATP...
  34. pmc Acute inhibition of PI3K-PDK1-Akt pathway potentiates insulin secretion through upregulation of newcomer granule fusions in pancreatic β-cells
    Kyota Aoyagi
    Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan
    PLoS ONE 7:e47381. 2012
    ..These data suggest that the PI3K-PDK1-Akt pathway plays a significant role in newcomer granule fusions, probably through an alteration of the dynamics of the intracellular insulin granules...
  35. ncbi request reprint Physiologic role of decidual beta1 integrin and focal adhesion kinase in embryonic implantation
    Hideki Hanashi
    Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Endocr J 50:189-98. 2003
    ..Thus, it was concluded that human decidual beta1 integrin and FAK participate in this final step of implantation...
  36. ncbi request reprint [Visualizing exocytosis of insulin granules with evanescent wave microscopy]
    Mica Ohara-Imaizumi
    Department of Biochemistry II, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611
    Seikagaku 75:49-54. 2003
  37. ncbi request reprint Elevation of the post-translational modification of proteins by O-linked N-acetylglucosamine leads to deterioration of the glucose-stimulated insulin secretion in the pancreas of diabetic Goto-Kakizaki rats
    Yoshihiro Akimoto
    Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Glycobiology 17:127-40. 2007
    ..These results indicate that elevation of the O-GlcNAcylation of proteins leads to deterioration of insulin secretion in the pancreas of diabetic GK rats, further providing evidence for the role of O-GlcNAc in the insulin secretion...
  38. ncbi request reprint Pancreatic endocrine function and glucose transporter (GLUT)-2 expression in rat acute pancreatitis
    Nobutsugu Abe
    First Department of Surgery, Kyorin University School of Medicine, Mitaka City, Tokyo, Japan
    Pancreas 25:149-53. 2002
    ..Impairment in pancreatic endocrine function is believed to play an important role in the development of glucose intolerance in acute pancreatitis...
  39. ncbi request reprint Osteoclastic function is accelerated in male patients with type 2 diabetes mellitus: the preventive role of osteoclastogenesis inhibitory factor/osteoprotegerin (OCIF/OPG) on the decrease of bone mineral density
    Kiyoshi Suzuki
    Third Department of Internal Medicine, Kyorin University School of Medicine, 6 20 2 Shinkawa, Mitaka, Tokyo 181 8611, Japan
    Diabetes Res Clin Pract 68:117-25. 2005
    ..It seems probable that OCIF/OPG has a suppressive role on the increased bone resorption to prevent further loss of the skeletal bone mass in type 2 diabetic patients...
  40. ncbi request reprint Small guanosine triphospatase RhoA and Rho-associated kinase as regulators of trophoblast migration
    Shigetatsu Shiokawa
    Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    J Clin Endocrinol Metab 87:5808-16. 2002
    ..Suppression of RhoA-ROCK reduces trophoblast migration. These findings suggest that RhoA-ROCK signaling is a key regulator of trophoblast cell migration...
  41. ncbi request reprint Insulin exocytotic mechanism by imaging technique
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, USA
    J Biochem 140:1-5. 2006
    ..Here we review recent results of our studies into docking and fusion of insulin granules analyzed by TIRF system...
  42. doi request reprint DPP-4 inhibitor des-F-sitagliptin treatment increased insulin exocytosis from db/db mice β cells
    Shinya Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Biochem Biophys Res Commun 412:556-60. 2011
    ..Thus, our data demonstrated that 2-weeks des-F-sitagliptin-treatment increased the fusion events of insulin granules, probably via increased number of docked insulin granules and that of syntaxin 1 clusters...
  43. ncbi request reprint Regulation of resident and newcomer insulin granules by calcium and SNARE proteins
    Kyota Aoyagi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Front Biosci (Landmark Ed) 16:1197-210. 2011
    ..In this review we discuss the mechanism of insulin exocytosis mainly from a point of view of imaging techniques...
  44. ncbi request reprint TIRF microscopy analysis of the mechanism of insulin exocytosis
    Shinya Nagamatsu
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Endocr J 53:433-40. 2006
  45. ncbi request reprint Mastoparan stimulates GABA release from MIN6 cells: relationship between SNARE proteins and mastoparan action
    M Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan
    Biochem Biophys Res Commun 289:1025-30. 2001
    ..Our data indicate that mastoparan stimulates GABA exocytosis via vesicular transport; however, SNARE proteins are differently involved in the exocytosis of insulin and GABA...
  46. ncbi request reprint Ménétrier's disease in a patient with Helicobacter pylori infection is linked to elevated glucagon-like peptide-2 activity
    Kazuhiro Watanabe
    Third Department of Internal Medicine, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Scand J Gastroenterol 40:477-81. 2005
    ..These observations suggest a possible link between excess systemic endogenous production of GLP-2, a gut hormone that induces mucosal growth, and the hypertrophic gastropathy in a Ménétrier's disease patient with H. pylori infection...
  47. ncbi request reprint Acute inhibition of proinsulin biosynthesis at the translational level by palmitic acid
    H Katahira
    Third Department of Internal Medicine, Kyorin University School of Medicine, Tokyo, Japan
    Biochem Biophys Res Commun 282:507-10. 2001
    ..Thus, our data indicate that PA primarily suppresses glucose-induced proinsulin biosynthesis within 1 h at the translational level...
  48. ncbi request reprint Renin-angiotensin system modulates oxidative stress-induced endothelial cell apoptosis in rats
    Masahiro Akishita
    Department of Geriatric Medicine, Kyorin University School of Medicine, Tokyo, Japan
    Hypertension 45:1188-93. 2005
    ..These results suggest that AT1 receptor signaling augments EC apoptosis in the process of oxidative stress-induced vascular injury...
  49. ncbi request reprint Imaging exocytosis of single insulin secretory granules with evanescent wave microscopy: distinct behavior of granule motion in biphasic insulin release
    Mica Ohara-Imaizumi
    Department of Biochemistry and the Department of Internal Medicine III, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    J Biol Chem 277:3805-8. 2002
    ..Thus, our data revealed the distinct behavior of the insulin granule motion during the 1st and 2nd phase of release...
  50. ncbi request reprint A prospective randomized trial of either famotidine or omeprazole for the prevention of bleeding after endoscopic mucosal resection and the healing of endoscopic mucosal resection-induced ulceration
    Y Yamaguchi
    The Third Department of Internal Medicine, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Aliment Pharmacol Ther 21:111-5. 2005
    ..However, uncertain whether an histamine2-receptor antagonist or proton-pump inhibitor is more effective...
  51. doi request reprint Effects of exercise training on adipogenesis of stromal-vascular fraction cells in rat epididymal white adipose tissue
    T Sakurai
    Department of Molecular Predictive Medicine and Sport Science, Kyorin University, Tokyo, Japan
    Acta Physiol (Oxf) 200:325-38. 2010
    ....
  52. ncbi request reprint Increases in plasma ACTH and cortisol after hypertonic saline infusion in patients with central diabetes insipidus
    E Itagaki
    Third Department of Internal Medicine, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    J Clin Endocrinol Metab 86:5749-54. 2001
    ..It seems possible that the increase in plasma osmolality promotes ACTH secretion in DI patients through AVP and/or urocortin via the hypophyseal portal system, independent of the AVP secretion from magnocellular neurons...
  53. ncbi request reprint Activation and apoptosis of murine peritoneal macrophages by acute cold stress
    T Kizaki
    Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Tokyo, Mitaka, 181 8611, Japan
    Biochem Biophys Res Commun 283:700-6. 2001
    ..DNA fragmentation and Annexin-V(+) cells were observed in peritoneal exudate cells from acute-cold stressed mice. Thus, cold stress activated macrophages but these macrophages were destined to be eliminated by apoptosis...
  54. ncbi request reprint Submucosal tumor (SMT)-like esophageal squamous cell carcinoma with gastric metastasis
    T Kishino
    Third Department of Internal Medicine, Kyorin University School of Medicine, 6 20 2, Shinkawa, Mitaka shi, Tokyo, 181 8611, Japan
    Hepatogastroenterology 47:1581-4. 2000
    ..It is necessary to recognize this complication so that appropriate therapy can be carried out on patients with esophageal cancer, and the situation needs to be carefully evaluated, including the stomach, both before and after treatment...
  55. ncbi request reprint Negative regulation of LPS-stimulated expression of inducible nitric oxide synthase by AP-1 in macrophage cell line J774A.1
    T Kizaki
    Department of Molecular Predictive Medicine and Sport Science, Kyorin University, Mitaka, Japan
    Biochem Biophys Res Commun 289:1031-8. 2001
    ....
  56. ncbi request reprint Characterization of morphological and cytoskeletal changes in trophoblast cells induced by insulin-like growth factor-I
    Maryam Kabir-Salmani
    Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    J Clin Endocrinol Metab 87:5751-9. 2002
    ....
  57. ncbi request reprint Structure, evolution, and liver-specific expression of sterol 12alpha-hydroxylase P450 (CYP8B)
    H Ishida
    Department of Biochemistry, Hiroshima University School of Dentistry, Minami Ku, Hiroshima, 734 8553, Japan
    J Biochem 126:19-25. 1999
    ..The enhanced CYP8B mRNA expression in streptozotocin-induced diabetic rats was significantly decreased by insulin within 3 h of its administration. These facts demonstrate a regulatory role of insulin in CYP8B expression as a suppressor...
  58. ncbi request reprint Helicobacter pylori and acetylsalicylic acid synergistically accelerate apoptosis via Fas antigen pathway in rabbit gastric epithelial cells
    Kazuhiro Watanabe
    Third Department of Internal Medicine, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
    Dig Dis Sci 47:809-17. 2002
    ..The results suggest that H. pylori and NSAID induce gastric cell injury as apoptosis via the Fas antigen pathway in a synergistic manner and that this effect is particularly strong when NSAID treatment follows H. pylori infection...
  59. pmc Comparative study of endotoxin-induced hypotension in kininogen-deficient rats with that in normal rats
    A Ueno
    Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
    Br J Pharmacol 114:1250-6. 1995
    ..In contrast, formation of endogenous PAF contributes to both the acute and the delayed hypotension afforded by endotoxin in vivo...
  60. pmc Role of inflammatory mediators in lipid A analogue (ONO-4007)-induced vascular permeability change in mouse skin
    H Ishida
    Department of Anesthesiology, Nippon Medical School, Tokyo, Japan
    Br J Pharmacol 130:1235-40. 2000
    ..Although ONO-4007 mimics LPS in increasing vascular permeability, mechanisms of permeability change elicited by ONO-4007 were not identical to those of LPS...
  61. ncbi request reprint Endoscopic treatment of hemorrhagic gastric ulcer in patients aged 80 years or more
    Y Yamaguchi
    Third Department of Internal Medicine, Kyorin University School of Medicine, Tokyo, Japan 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan
    Hepatogastroenterology 48:1195-8. 2001
    ..Endoscopic hemostasis for hemorrhagic gastric ulcer is effective and safe, even for such elderly patients...
  62. ncbi request reprint Expression of membrane-bound transferrin-like protein p97 on the cell surface of chondrocytes
    T Kawamoto
    Department of Biochemistry, Hiroshima University School of Dentistry, Japan
    Eur J Biochem 256:503-9. 1998
    ..p97 transcript was detected in all zones of the cartilage but the level was relatively low in the hypertrophic zone. These findings suggest that p97 is involved in maintaining the cell surface characteristics of chondrocytes...
  63. ncbi request reprint Endoscopic hemoclipping for upper GI bleeding due to Mallory-Weiss syndrome
    Y Yamaguchi
    Third Department of Internal Medicine, Kyorin University School of Medicine, Tokyo, Japan
    Gastrointest Endosc 53:427-30. 2001
    ..CONCLUSION: Endoscopic hemoclipping provided an effective and safe modality for obtaining hemostasis when bleeding is due to MWS...
  64. ncbi request reprint Dissection of the effects of tumor necrosis factor-alpha and class II gene polymorphisms within the MHC on murine systemic lupus erythematosus (SLE)
    T Fujimura
    Department of Pathology, Juntendo University School of Medicine, Tokyo, Japan
    Int Immunol 10:1467-72. 1998
    ..It seems likely that both class II and TNF-alpha gene polymorphisms are functioning as H-2-linked predisposing genetic elements, and that the TNF-alpha polymorphism acts to modulate an initial process of the renal disease...
  65. ncbi request reprint Molecular cloning of a novel alpha2,3-sialyltransferase (ST3Gal VI) that sialylates type II lactosamine structures on glycoproteins and glycolipids
    T Okajima
    Department of Biochemistry, Nagoya University School of Medicine, Tsurumai, Nagoya 466 0065, Japan
    J Biol Chem 274:11479-86. 1999
    ..Consequently, these data indicated that this enzyme is involved in the synthesis of sialyl-paragloboside, a precursor of sialyl-Lewis X determinant...
  66. ncbi request reprint Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase
    H Ishida
    Department of Biochemistry, Hiroshima University School of Dentistry, Japan
    J Biol Chem 267:21319-23. 1992
    ..However, an immunoblotting experiment showed no correlation between the enzyme activity and the amount of protein, suggesting that post-translational modification may occur...
  67. ncbi request reprint A lipid A analog ONO-4007 induces tolerance to plasma leakage in mice
    H Ishida
    Department of Anesthesiology, Nippon Medical School, Tokyo, Japan
    Inflamm Res 51:38-43. 2002
    ..The effects of pretreatment with ONO-4007, a lipid A analog, on cutaneous plasma leakage induced by ONO-4007, lipopolysaccharide (LPS) and inflammatory mediators were investigated...
  68. ncbi request reprint Efficacy of IgM anti-blood type antibody monitoring by enzyme-linked immunosorbent assay after renal transplantation across the blood barrier: high-dose immunoglobulin administration blocks IgM rather than IgG anti-blood type antibodies
    H Ishida
    Department of Urology, Tokyo Women s Medical University, Tokyo, Japan
    Transplant Proc 36:2178-81. 2004
    ..Blocking of IgM seems to play an important role in improving ABO-incompatible grafts...
  69. pmc Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation
    Kazuo Kasai
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    J Clin Invest 115:388-96. 2005
    ....
  70. ncbi request reprint [Visualizing exocytosis of insulin granules with total internal reflection fluorescence microscopy]
    Mica Ohara-Imaizumi
    Nihon Yakurigaku Zasshi 126:399-405. 2005
  71. pmc Secretory granules are recaptured largely intact after stimulated exocytosis in cultured endocrine cells
    Justin W Taraska
    Vollum Institute, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
    Proc Natl Acad Sci U S A 100:2070-5. 2003
    ..Reacidification and dimming could be reversed by application of NH(4)Cl. We conclude that most granules reseal in <10 s after releasing cargo, and that these empty or partially empty granules are recaptured otherwise intact...
  72. ncbi request reprint Rab27 effector granuphilin promotes the plasma membrane targeting of insulin granules via interaction with syntaxin 1a
    Seiji Torii
    Laboratory of Gene Analysis and Laboratory of Gene Engineering, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371 8512, Japan
    J Biol Chem 279:22532-8. 2004
    ..The complex formation of these three proteins may contribute to the specificity of the targeting process during the exocytosis of insulin granules...
  73. ncbi request reprint [Molecular mechanism of insulin exocytosis and the role of SNARE proteins]
    Shinya Nagamatsu
    Department of Biochemistry II, Kyorin University School of Medicine
    Nihon Rinsho 60:213-8. 2002
  74. ncbi request reprint Protein kinase Cdelta plays a non-redundant role in insulin secretion in pancreatic beta cells
    Toyoyoshi Uchida
    Department of Medicine, Metabolism and Endocrinology, Juntendo University School of Medicine, 2 1 1, Tokyo 113 8421, Japan
    J Biol Chem 282:2707-16. 2007
    ..We conclude that PKCdelta plays a non-redundant role in glucose-stimulated insulin secretion. The impaired insulin secretion in PKCdelta null mice is associated with reduced phosphorylation of Munc18-1...
  75. ncbi request reprint EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets
    Irena Konstantinova
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Cell 129:359-70. 2007
    ..Thus, we explain how beta cell communication in pancreatic islets conversely affects basal and glucose-stimulated insulin secretion to improve glucose homeostasis...
  76. ncbi request reprint Decreased marble burying behavior in female mice lacking neuromedin-B receptor (NMB-R) implies the involvement of NMB/NMB-R in 5-HT neuron function
    Kazuyuki Yamada
    Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187 8502, Japan
    Brain Res 942:71-8. 2002
    ..These behavioral and biological results suggest that NMB/NMB-R may modulate 5-HT neuronal activity by affecting DR function...
  77. ncbi request reprint Maturity-onset diabetes of the young resulting from a novel mutation in the HNF-4alpha gene
    Kazuo Hara
    Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo
    Intern Med 41:848-52. 2002
    ..Genetic testing enabled early diagnosis of diabetes in the patient's 20-year-old daughter, which we consider to be important for the prevention of diabetic complications...