Research Topics
Genomes and Genes | Yoshihiro AkimotoSummaryAffiliation: Kyorin University Country: Japan Publications
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Publications
Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including α-actinin 4Yoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
Clin Proteomics 8:15. 2011..abstract:..
Elevation of the post-translational modification of proteins by O-linked N-acetylglucosamine leads to deterioration of the glucose-stimulated insulin secretion in the pancreas of diabetic Goto-Kakizaki ratsYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Glycobiology 17:127-40. 2007..These results indicate that elevation of the O-GlcNAcylation of proteins leads to deterioration of insulin secretion in the pancreas of diabetic GK rats, further providing evidence for the role of O-GlcNAc in the insulin secretion...
Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephrotic syndromeMariko Shimizu
Department of Pediatrics, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Lab Invest 89:178-95. 2009....
Insulin exocytosis in Goto-Kakizaki rat beta-cells subjected to long-term glinide or sulfonylurea treatmentJunko Kawai
Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
Biochem J 412:93-101. 2008..We conclude that treatment of GK rats with nateglinide and glibenclamide varies in long-term effects on beta-cell functions; nateglinide treatment appears overall to be more beneficial...
Change in long-spacing collagen in Descemet's membrane of diabetic Goto-Kakizaki rats and its suppression by antidiabetic agentsYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
Exp Diabetes Res 2008:818341. 2008..Long-spacing collagen would thus be a useful indicator for studying diabetic changes in the cornea and the effect of antidiabetic agents...
The membrane-spanning domain of CD98 heavy chain promotes alpha(v)beta3 integrin signals in human extravillous trophoblastsMaryam Kabir-Salmani
Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka shi, Tokyo 181 8611, Japan
Mol Endocrinol 22:707-15. 2008..These results suggest that notoriously invasive activity of extravillous trophoblast is mediated by CD98hc, which promotes alpha(v)beta(3) integrin-dependent signals...
Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosisMica Ohara-Imaizumi
Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan
PLoS ONE 5:e15553. 2010..Therefore, to determine the role of CDKAL1 in insulin release from β cells, we studied insulin release profiles in CDKAL1 gene knockout (CDKAL1 KO) mice...
Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosisMica Ohara-Imaizumi
Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
J Cell Biol 177:695-705. 2007..We conclude that the first phase is Synt1A dependent but the second phase is not. This indicates that the two phases of insulin exocytosis differ spatially and mechanistically...
Amino acid transporter LAT3 is required for podocyte development and functionYuji Sekine
Kyorin University School of Medicine, Department of Pediatrics, Mitaka, Tokyo 181 8611, Japan
J Am Soc Nephrol 20:1586-96. 2009..Our data suggest that LAT3 may play a crucial role in the development and maintenance of podocyte structure and function by regulating protein synthesis and the actin cytoskeleton...
Imaging exocytosis of single glucagon-like peptide-1 containing granules in a murine enteroendocrine cell line with total internal reflection fluorescent microscopyMica Ohara-Imaizumi
Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
Biochem Biophys Res Commun 390:16-20. 2009..Closely similar to the insulin granule fusion from pancreatic beta cells, the regulated biphasic exocytosis from two types of granules may be a common mechanism in glucose-evoked hormone release from endocrine cells...
The struggle for energy in podocytes leads to nephrotic syndromeKunimasa Yan
Department of Pediatrics, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Cell Cycle 11:1504-11. 2012..Finally, we will discuss the potential significance of targeting the energy metabolism of podocytes to develop new therapeutic interventions for acquired nephrotic syndrome...
mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndromeNoriko Ito
Department of Pediatrics, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Lab Invest 91:1584-95. 2011..In conclusion, mTORC1 activation may perturb the regulatory system of energy metabolism primarily by promoting energy consumption and inducing the UPR, which underlie proteinuria in MCD...
Tissue transglutaminase at embryo-maternal interfaceMaryam Kabir-Salmani
Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
J Clin Endocrinol Metab 90:4694-702. 2005....
Properties of L-type amino acid transporter 1 in epidermal ovarian cancerMasahiko Kaji
Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Int J Gynecol Cancer 20:329-36. 2010....
Physiologic role of decidual beta1 integrin and focal adhesion kinase in embryonic implantationHideki Hanashi
Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
Endocr J 50:189-98. 2003..Thus, it was concluded that human decidual beta1 integrin and FAK participate in this final step of implantation...
Role of amino acid transporter LAT2 in the activation of mTORC1 pathway and the pathogenesis of crescentic glomerulonephritisRyota Kurayama
Department of Pediatrics, Kyorin University School of Medicine, Tokyo, Japan
Lab Invest 91:992-1006. 2011..Our data suggest that LAT2 may have a pivotal role in the pathogenesis of CGN by activating the mTORC1 pathway in the glomerular epithelial cells...
Elevated expression of O-GlcNAc-modified proteins and O-GlcNAc transferase in corneas of diabetic Goto-Kakizaki ratsYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Invest Ophthalmol Vis Sci 44:3802-9. 2003....
ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence miMica Ohara-Imaizumi
Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan
Mol Biol Cell 16:3289-300. 2005..Moreover, attenuation of ELKS expression by small interfering RNA reduced the glucose-evoked insulin release. These data suggest that the CAZ-related protein ELKS functions in insulin exocytosis from pancreatic beta cells...
Small guanosine triphospatase RhoA and Rho-associated kinase as regulators of trophoblast migrationShigetatsu Shiokawa
Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
J Clin Endocrinol Metab 87:5808-16. 2002..Suppression of RhoA-ROCK reduces trophoblast migration. These findings suggest that RhoA-ROCK signaling is a key regulator of trophoblast cell migration...
Protein characterization of NA+-independent system L amino acid transporter 3 in mice: a potential role in supply of branched-chain amino acids under nutrient starvationDaisuke Fukuhara
Department of Pediatrics, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
Am J Pathol 170:888-98. 2007....
O-GlcNAc modification of nucleocytoplasmic proteins and diabetesYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, 181 8611, Japan
Med Mol Morphol 38:84-91. 2005..In this article, we review the current data regarding the relationship between O-GlcNAc modification and diabetes...
Changes in distribution of the long form of type XII collagen during chicken corneal developmentYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Tokyo, Japan
J Histochem Cytochem 50:851-62. 2002....
Pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectinYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan
Methods Mol Biol 657:217-24. 2010..This chapter describes a pre-embedding method for carbohydrate localization in which tissue sections are incubated with horseradish peroxidase-conjugated lectin prior to embedding in resin...
Characterization of morphological and cytoskeletal changes in trophoblast cells induced by insulin-like growth factor-IMaryam Kabir-Salmani
Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan
J Clin Endocrinol Metab 87:5751-9. 2002....
Localization of the O-GlcNAc transferase and O-GlcNAc-modified proteins in rat cerebellar cortexYoshihiro Akimoto
Department of Anatomy, Kyorin University School of Medicine, Mitaka, 181-8611, Tokyo, Japan
Brain Res 966:194-205. 2003..Thus, by modulating the phosphorylation or protein associations of key regulatory and cytoskeletal proteins, O-GlcNAc is likely important to many functions of the cerebellum...
Alphavbeta3 integrin signaling pathway is involved in insulin-like growth factor I-stimulated human extravillous trophoblast cell migrationMaryam Kabir-Salmani
Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan
Endocrinology 144:1620-30. 2003..These findings indicated alpha(v)beta(3) integrin localization in the core of focal adhesions of EVT cells and that alpha(v)beta(3) integrin signaling pathways are activated in IGF-I-mediated migration of these cells...
