Research Topics
Species | Toru TakeoSummaryAffiliation: Kumamoto University Location: Kumamoto, Japan Publications
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Detail Information
Publications
Methyl-beta-cyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cellsToru Takeo
Department of Clinical Chemistry and Informatics, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862 0973, Japan
Biol Reprod 78:546-51. 2008..In summary, we suggest that MBCD is useful for developing an IVF method for frozen/thawed C57BL/6 mouse sperm achieving a high fertilization rate, being involved in the capacity to sequester cholesterol from sperm membrane...
Decrease of fertilizing ability of mouse spermatozoa after freezing and thawing is related to cellular injuryHirofumi Nishizono
Kyudo Company Limited, Kumamoto 861-0104, Japan
Biol Reprod 71:973-8. 2004..7%; FR, 17.0%; BALB/cA: FAS, 67.2%; FR, 24.2%; and DBA/2N: FAS, 10.2%; FR, 93.6%), and damage to spermatozoa was localized particularly in the acrosome of the head and mitochondria...
LGR4 regulates the postnatal development and integrity of male reproductive tracts in miceTakayuki Hoshii
Division of Developmental Genetics, Institute of Molecular Embryology and Genetics, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860 0811, Japan
Biol Reprod 76:303-13. 2007..These results indicate that Lgr4 has pivotal roles to play in the regulation of ESR1 expression, the control of duct elongation through BM remodeling, and the regional differentiation of the caput epididymidis...
Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperatureToru Takeo
Division of Reproductive Engineering, Center for Animal Resources and Development CARD, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
Cryobiology 58:196-202. 2009..In summary, short-term storage and transport of vitrified/warmed 2-cell embryos in M2 medium at a cold temperature can maintain their ability to develop into live young...
Combination medium of cryoprotective agents containing L-glutamine and methyl-{beta}-cyclodextrin in a preincubation medium yields a high fertilization rate for cryopreserved C57BL/6J mouse spermT Takeo
Center for Animal Resources and Development, Kumamoto University, Japan
Lab Anim 44:132-7. 2010..Furthermore, the new method provides a reliable archiving and reproducing system for genetically-engineered mice using sperm cryopreservation...
Short-term storage and transport at cold temperatures of 2-cell mouse embryos produced by cryopreserved spermToru Takeo
Division of Reproductive Engineering, Center for Animal Resources and Development CARD, Kumamoto University, Kumamoto, Japan
J Am Assoc Lab Anim Sci 49:415-9. 2010....
Novel migrating mouse neural crest cell assay system utilizing P0-Cre/EGFP fluorescent time-lapse imagingMinoru Kawakami
Division of Developmental Genetics, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto City, Kumamoto, 860 0811, Japan
BMC Dev Biol 11:68. 2011..Their long-range and precision-guided migration is one of their most striking characteristics. We previously reported that P0-Cre/CAG-CAT-lacZ double-transgenic mice showed significant lacZ expression in tissues derived from NCCs...
