Ryuichi Nishinakamura


Affiliation: Kumamoto University
Country: Japan


  1. Yoshimura Y, Taguchi A, Tanigawa S, Yatsuda J, Kamba T, Takahashi S, et al. Manipulation of Nephron-Patterning Signals Enables Selective Induction of Podocytes from Human Pluripotent Stem Cells. J Am Soc Nephrol. 2019;30:304-321 pubmed publisher
    ..These PSC-derived podocytes show molecular, morphologic, and functional characteristics of podocytes, and offer a new resource for disease modeling and nephrotoxicity testing. ..
  2. Yoshimura Y, Taguchi A, Nishinakamura R. Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells. Methods Mol Biol. 2019;1926:87-102 pubmed publisher
  3. Murakami Y, Naganuma H, Tanigawa S, Fujimori T, Eto M, Nishinakamura R. Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation. Sci Rep. 2019;9:1172 pubmed publisher
    ..Thus, our protocol will serve as a useful platform for identification of renal endothelial precursors and induction of these precursors from pluripotent stem cells. ..
  4. Tanigawa S, Islam M, Sharmin S, Naganuma H, Yoshimura Y, Haque F, et al. Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes. Stem Cell Reports. 2018;11:727-740 pubmed publisher
    ..Thus, these kidney organoids from patient-derived iPSCs identified SD abnormalities in the podocytes at the initial phase of congenital nephrotic disease. ..
  5. Sharmin S, Taguchi A, Kaku Y, Yoshimura Y, Ohmori T, Sakuma T, et al. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation. J Am Soc Nephrol. 2016;27:1778-91 pubmed publisher
    ..Therefore, the podocytes generated from iPS cells retain the podocyte-specific molecular and structural features, which will be useful for dissecting human glomerular development and diseases. ..
  6. Tanigawa S, Taguchi A, Sharma N, Perantoni A, Nishinakamura R. Selective In Vitro Propagation of Nephron Progenitors Derived from Embryos and Pluripotent Stem Cells. Cell Rep. 2016;15:801-813 pubmed publisher
    ..Thus, we have established in vitro conditions for promoting the propagation of nephron progenitors, which will be essential for dissecting the mechanisms of kidney organogenesis and for regenerative medicine. ..
  7. Nishinakamura R, Sharmin S, Taguchi A. Induction of nephron progenitors and glomeruli from human pluripotent stem cells. Pediatr Nephrol. 2017;32:195-200 pubmed publisher
    ..Other laboratories have reported different protocols to induce nephron structures from human iPSCs in vitro. These findings will accelerate our understanding of kidney development and diseases in humans. ..
  8. Tanigawa S, Nishinakamura R. Expanding nephron progenitors in vitro: a step toward regenerative medicine in nephrology. Kidney Int. 2016;90:925-927 pubmed publisher
    ..A recent publication reports the first successful attempt to propagate human nephron progenitors while retaining their potential to form both glomeruli and renal tubules. ..
  9. Yoshimura Y, Taguchi A, Nishinakamura R. Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells. Methods Mol Biol. 2017;1597:179-193 pubmed publisher
    ..We also provide a method to generate 3D kidney tissue with vascularized glomeruli upon transplantation. ..

More Information


  1. Haque F, Kaku Y, Fujimura S, Ohmori T, Adelstein R, Nishinakamura R. Non-muscle myosin II deletion in the developing kidney causes ureter-bladder misconnection and apical extrusion of the nephric duct lineage epithelia. Dev Biol. 2017;427:121-130 pubmed publisher
    ..Thus, myosin II is essential for maintaining the apicobasal integrity of the developing kidney epithelia independently of Ret signaling. ..
  2. Taguchi A, Kaku Y, Ohmori T, Sharmin S, Ogawa M, Sasaki H, et al. Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells. Cell Stem Cell. 2014;14:53-67 pubmed publisher
    ..Thus, by reevaluating the developmental origins of metanephric progenitors, we have provided key insights into kidney specification in vivo and taken important steps toward kidney organogenesis in vitro. ..
  3. Recuenco M, Ohmori T, Tanigawa S, Taguchi A, Fujimura S, Conti M, et al. Nonmuscle Myosin II Regulates the Morphogenesis of Metanephric Mesenchyme-Derived Immature Nephrons. J Am Soc Nephrol. 2015;26:1081-91 pubmed publisher
    ..Our data also suggest that Myh9 deletion in mice results in failure to maintain renal tubules but not in glomerulosclerosis. ..
  4. Kaku Y, Taguchi A, Tanigawa S, Haque F, Sakuma T, Yamamoto T, et al. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells. Sci Rep. 2017;7:4554 pubmed publisher
  5. Taguchi A, Nishinakamura R. Higher-Order Kidney Organogenesis from Pluripotent Stem Cells. Cell Stem Cell. 2017;21:730-746.e6 pubmed publisher
    ..This selective induction and reassembly strategy will be a powerful approach to recapitulate organotypic architecture in PSC-derived organoids. ..
  6. Chiga M, Ohmori T, Ohba T, Katabuchi H, Nishinakamura R. Preformed Wolffian duct regulates Müllerian duct elongation independently of canonical Wnt signaling or Lhx1 expression. Int J Dev Biol. 2014;58:663-8 pubmed publisher
    ..These results suggest that the Wolffian duct regulates Müllerian duct elongation by currently unidentified mechanisms that are independent of canonical Wnt signaling or Lhx1 expression. ..
  7. Kanda S, Tanigawa S, Ohmori T, Taguchi A, Kudo K, Suzuki Y, et al. Sall1 maintains nephron progenitors and nascent nephrons by acting as both an activator and a repressor. J Am Soc Nephrol. 2014;25:2584-95 pubmed publisher
  8. Nishinakamura R, Takasato M. Human development, heredity and evolution. Development. 2017;144:2099-2103 pubmed publisher
    ..This Meeting Review provides a summary of this timely and exciting symposium, which has convinced us that we are moving into the era of science targeted on humans. ..