Toshiharu Yagi

Summary

Affiliation: Kochi University
Country: Japan

Publications

  1. ncbi request reprint Contents of all forms of vitamin B6, pyridoxine-β-glucoside and 4-pyridoxic acid in mature milk of Japanese women according to 4-pyridoxolactone-conversion high performance liquid chromatography
    Toshiharu Yagi
    Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, Japan
    J Nutr Sci Vitaminol (Tokyo) 59:9-15. 2013
  2. ncbi request reprint Development of simultaneous enzymatic assay method for all six individual vitamin B6 forms and pyridoxine-beta-glucoside
    Toshiharu Yagi
    Faculty of Agriculture, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, Japan
    J Nutr Sci Vitaminol (Tokyo) 56:157-63. 2010
  3. ncbi request reprint Identification of a new tetrameric pyridoxal 4-dehydrogenase as the second enzyme in the degradation pathway for pyridoxine in a nitrogen-fixing symbiotic bacterium, Mesorhizobium loti
    Nana Yokochi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    Arch Biochem Biophys 452:1-8. 2006
  4. ncbi request reprint Gene identification and characterization of the pyridoxine degradative enzyme alpha-(N-acetylaminomethylene)succinic acid amidohydrolase from Mesorhizobium loti MAFF303099
    Baiqiang Yuan
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi, Japan
    J Nutr Sci Vitaminol (Tokyo) 54:185-90. 2008
  5. doi request reprint Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase
    Nana Yokochi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    J Biochem 145:493-503. 2009
  6. ncbi request reprint Molecular cloning, expression, and properties of an unusual aldo-keto reductase family enzyme, pyridoxal 4-dehydrogenase, that catalyzes irreversible oxidation of pyridoxal
    Nana Yokochi
    Department of Bioresources Science, Faculty of Agriculture and Research Institute of Molecular Genetics, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    J Biol Chem 279:37377-84. 2004
  7. ncbi request reprint Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti MAFF303099
    Yu Yoshikane
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    J Biol Chem 283:1120-7. 2008
  8. doi request reprint Gene identification and characterization of the pyridoxine degradative enzyme 4-pyridoxic acid dehydrogenase from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti MAFF303099
    Fei Ge
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783 8502, Japan
    J Biochem 143:603-9. 2008
  9. ncbi request reprint Determination of individual vitamin B(6) compounds based on enzymatic conversion to 4-pyridoxolactone
    Saki Nishimura
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Kochi, Japan
    J Nutr Sci Vitaminol (Tokyo) 54:18-24. 2008
  10. ncbi request reprint Disruption of the plr1+ gene encoding pyridoxal reductase of Schizosaccharomyces pombe
    Tomotake Morita
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783 8502
    J Biochem 135:225-30. 2004

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Contents of all forms of vitamin B6, pyridoxine-β-glucoside and 4-pyridoxic acid in mature milk of Japanese women according to 4-pyridoxolactone-conversion high performance liquid chromatography
    Toshiharu Yagi
    Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, Japan
    J Nutr Sci Vitaminol (Tokyo) 59:9-15. 2013
    ..The total content of vitamin B6 forms, and predominant presence of pyridoxal among other vitamin B6 forms in the Japanese women's milk samples shared similar characteristics with American women's milk samples...
  2. ncbi request reprint Development of simultaneous enzymatic assay method for all six individual vitamin B6 forms and pyridoxine-beta-glucoside
    Toshiharu Yagi
    Faculty of Agriculture, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, Japan
    J Nutr Sci Vitaminol (Tokyo) 56:157-63. 2010
    ..The contents of 4-pyridoxic acid and pyridoxal correlate well with a correlation coefficient of 0.98. On the other hand, the content of pyridoxamine did not correlate with that of 4-pyridoxic acid...
  3. ncbi request reprint Identification of a new tetrameric pyridoxal 4-dehydrogenase as the second enzyme in the degradation pathway for pyridoxine in a nitrogen-fixing symbiotic bacterium, Mesorhizobium loti
    Nana Yokochi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    Arch Biochem Biophys 452:1-8. 2006
    ..Thus, it was found that PLDH is essential for the assimilation of vitamin B6 compounds and the second step enzyme in their degradation pathway in M. loti...
  4. ncbi request reprint Gene identification and characterization of the pyridoxine degradative enzyme alpha-(N-acetylaminomethylene)succinic acid amidohydrolase from Mesorhizobium loti MAFF303099
    Baiqiang Yuan
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi, Japan
    J Nutr Sci Vitaminol (Tokyo) 54:185-90. 2008
    ....
  5. doi request reprint Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase
    Nana Yokochi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    J Biochem 145:493-503. 2009
    ..The E149Q one was stable in contrast to the corresponding human 3-hydroxyacyl-CoA dehydrogenase mutant, and showed unique pH optima depending on the co-substrates used for the reaction...
  6. ncbi request reprint Molecular cloning, expression, and properties of an unusual aldo-keto reductase family enzyme, pyridoxal 4-dehydrogenase, that catalyzes irreversible oxidation of pyridoxal
    Nana Yokochi
    Department of Bioresources Science, Faculty of Agriculture and Research Institute of Molecular Genetics, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    J Biol Chem 279:37377-84. 2004
    ..Furthermore, pyridoxal 4-dehydrogenase prefers NAD(+) to NADP(+) as a cofactor, although AKRs generally show higher affinities for the latter...
  7. ncbi request reprint Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti MAFF303099
    Yu Yoshikane
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    J Biol Chem 283:1120-7. 2008
    ..The reaction mechanism of the enzyme is discussed based on the structures and kinetics results...
  8. doi request reprint Gene identification and characterization of the pyridoxine degradative enzyme 4-pyridoxic acid dehydrogenase from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti MAFF303099
    Fei Ge
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783 8502, Japan
    J Biochem 143:603-9. 2008
    ..5 and 30 degrees C, and 29 muM, respectively. The enzyme was a glucose-methanol-choline (GMC) family protein with two signature patterns, FAD-binding residues, a putative active site histidine residue and a probable transmembrane segment...
  9. ncbi request reprint Determination of individual vitamin B(6) compounds based on enzymatic conversion to 4-pyridoxolactone
    Saki Nishimura
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Kochi, Japan
    J Nutr Sci Vitaminol (Tokyo) 54:18-24. 2008
    ..Pyridoxine 5'-phosphate and pyridoxine-beta-glucoside were not separately determined here. Three food samples were analyzed by this method...
  10. ncbi request reprint Disruption of the plr1+ gene encoding pyridoxal reductase of Schizosaccharomyces pombe
    Tomotake Morita
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783 8502
    J Biochem 135:225-30. 2004
    ..The results showed that PL reductase encoded by the plr1(+ )gene is involved in the excretion of PL after reducing it to PN, and may not participate in the salvage pathway for PLP synthesis...
  11. ncbi request reprint Molecular cloning, identification and characterization of 2-methyl-3-hydroxypyridine-5-carboxylic-acid-dioxygenase-coding gene from the nitrogen-fixing symbiotic bacterium Mesorhizobium loti
    Baiqiang Yuan
    Department of Bioresources Science, Faculty of Agriculture, Nankoku, Kochi 783 8502, Japan
    J Biosci Bioeng 102:504-10. 2006
    ..From the infection experiment of a symbiotic plant Lotus japonicus with an M. loti mlr6788 gene disruptant, MHPCO was demonstrated to be dispensable for at least nodule formation on roots of seedlings in symbiosis...
  12. doi request reprint Synthesis of 4-pyridoxolactone from pyridoxine using a combination of transformed Escherichia coli cells
    Asuka Tamura
    Department of Agriculture, Faculty of Agriculture, Kochi University, 200 Monobe Otsu, Nankoku, Kochi, Japan
    J Biosci Bioeng 106:460-5. 2008
    ..When starting with 80 mM of pyridoxine, it was necessary to add 0.5 mM or more of NAD(+) to complete the reaction...
  13. ncbi request reprint The nitrogen-fixing symbiotic bacterium Mesorhizobium loti has and expresses the gene encoding pyridoxine 4-oxidase involved in the degradation of vitamin B6
    Baiqiang Yuan
    Department of Bioresources Science, Faculty of Agriculture, Nankoku, Kochi 783 8502, Japan
    FEMS Microbiol Lett 234:225-30. 2004
    ..M. loti grown in a Rhizobium medium showed the enzyme activity. The results suggest that M. loti also contains the degradation pathway of vitamin B6...
  14. doi request reprint Crystal structure of pyridoxine 4-oxidase from Mesorhizobium loti
    Andrew Njagi Mugo
    Graduate School of Integral Arts and Science, Kochi University, Nankoku, Kochi, Japan
    Biochim Biophys Acta 1834:953-63. 2013
    ..The function of the novel proline residue was discussed...
  15. pmc Crystallization and preliminary X-ray analysis of 4-pyridoxolactonase from Mesorhizobium loti
    Sayoko Matsuda
    Faculty of Agriculture, Graduate School of Integral Arts and Science, Kochi University, Monobe, Nankoku, Kochi, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:886-9. 2009
    ..24, b = 39.35, c = 82.68 A, beta = 118.02 degrees. The calculated V(M) values suggested that the asymmetric unit contains one molecule in both crystal forms...
  16. pmc Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway I of pyridoxine
    Jun Kobayashi
    Faculty of Agriculture and Agricultural Science Program, Graduate School of Integral Arts and Science, Kochi University, Nankoku, Kochi 783 8502, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:829-31. 2009
    ..4 degrees , and diffraction data were collected to 2.7 A resolution. The V(M) value and calculation of the self-rotation function suggested that three dimers with a threefold symmetry were possibly present in the asymmetric unit...
  17. pmc Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti
    Huy Nhat Chu
    Faculty of Agriculture and Agricultural Science Program, Graduate School of Integral Arts and Science, Kochi University, Monobe, Nankoku, Kochi 783 8502, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:718-20. 2010
    ..9 A resolution and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 86.20, b = 51.11, c = 91.73 A, beta = 89.36 degrees. The calculated V(M) values suggested that the asymmetric unit contained four molecules...
  18. pmc Crystallization and preliminary X-ray analysis of pyridoxine 4-oxidase, the first enzyme in pyridoxine degradation pathway I
    Andrew Njagi Mugo
    Faculty of Agriculture and Agricultural Science Program, Graduate School of Integral Arts and Science, Kochi University, Nankoku, Kochi 783 8502, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:66-8. 2012
    ..38, b = 79.44, c = 136.43 Å, diffracted to 2.2 Å resolution. The calculated V(M) value (3.19 Å(3) Da(-1)) suggested that the asymmetric unit contained one molecule...
  19. ncbi request reprint 4-Pyridoxolactonase from a symbiotic nitrogen-fixing bacterium Mesorhizobium loti: cloning, expression, and characterization
    Junichi Funami
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    Biochim Biophys Acta 1753:234-9. 2005
    ..The alignment of amino acid sequences suggests that 4-pyridoxolactonase has three histidine residues probably involved in binding of Zn2+...
  20. ncbi request reprint Coenzyme precursor-assisted cooperative overexpression of an active pyridoxine 4-oxidase from Microbacterium luteolum
    Yu Yoshikane
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    Protein Expr Purif 34:243-8. 2004
    ..The overexpressed pyridoxine 4-oxidase was easily purified to homogeneity with two steps of the conventional column chromatography...
  21. doi request reprint The mll6786 gene encodes a repressor protein controlling the degradation pathway for vitamin B6 in Mesorhizobium loti
    Takayuki Nagase
    Faculty of Agriculture and Agricultural Science Program, Graduate School of Integral Arts and Science, Kochi University, Nankoku, Kochi, Japan
    FEMS Microbiol Lett 329:116-22. 2012
    ....
  22. ncbi request reprint The crystal structure of SDR-type pyridoxal 4-dehydrogenase of Mesorhizobium loti
    Huy Nhat Chu
    Faculty of Agriculture and Agricultural Science Program, Graduate School of Integral Arts and Science, Kochi University, Nankoku, Kochi, Japan
    Biosci Biotechnol Biochem 75:388-90. 2011
    ..Its crystal structure was elucidated for the first time. Molecular replacement with (S)-1-phenylthanol dehydrogenase (PDB code 2EW8) was adopted to determine the tertiary structure of the NAD(+)-bound enzyme...
  23. doi request reprint Topology of 4-Pyridoxic acid dehydrogenase in transformed Escherichia coli cells
    Fei Ge
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783 8502, Japan
    J Biochem 147:291-5. 2010
    ..coli cell membrane. Thus, it was suggested that the intact deduced transmembrane segment was necessary for 4-pyridoxic acid dehydrogenase to be localized on the bacterial cell membrane...
  24. pmc Molecular cloning, expression and characterization of pyridoxamine-pyruvate aminotransferase
    Yu Yoshikane
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    Biochem J 396:499-507. 2006
    ..The active-site structure and evolutionary aspects of the enzyme are discussed...
  25. ncbi request reprint Inhibition of diphenolase activity of tyrosinase by vitamin b(6) compounds
    Nana Yokochi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu 200, Nankoku, Kochi 783 8502, Japan
    J Agric Food Chem 51:2733-6. 2003
    ..AAP was also a mixed-type inhibitor. The results showed that the vitamin B(6) compounds inhibited the diphenolase activity by quenching ROS (probably singlet oxygen) generated during some reaction step of the diphenolase reaction...
  26. ncbi request reprint Crystallization and preliminary X-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway I of pyridoxine
    Nana Yokochi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783 8502, Japan
    Acta Crystallogr D Biol Crystallogr 60:2061-2. 2004
    ..The crystals were monoclinic and belonged to space group C2, with unit-cell parameters a = 107.0, b = 56.7, c = 130.2 A, beta = 103.6 degrees . Diffraction data were collected from a single crystal to 2.0 A...
  27. doi request reprint Individual vitamin B6 contents in selected Japanese sushi toppings
    Huong T V Do
    Faculty of Agriculture and Agricultural Science Program, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi, Japan
    Int J Food Sci Nutr 63:246-9. 2012
    ..Five portions of sushi with 20 g of fatty seawater sashimi toppings would supply with vitamin B(6) recommended by the Japanese Recommended Daily Allowance...
  28. doi request reprint Abnormality in expression levels of gluconeogenesis-related genes by high-dose supplementation with pyridoxamine in mice
    Huong Thi Viet Do
    Faculty of Agriculture and Agricultural Science Program, Graduate School of Integral Arts and Science, Kochi University, Nankoku, Kochi, Japan
    Int J Vitam Nutr Res 82:34-40. 2012
    ..Pyruvate challenge testing suggested that pyridoxamine supplementation enhanced gluconeogenic activity from pyruvate. The results showed that a high-dose of pyridoxamine may require a careful inquiry concerning its validity...
  29. ncbi request reprint Characterization of recombinant YakC of Schizosaccharomyces pombe showing YakC defines a new family of aldo-keto reductases
    Tomotake Morita
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Monobe Otsu Nankoku, Kochi 783 8502, Japan
    J Biochem 132:635-41. 2002
    ....
  30. ncbi request reprint An enzymatic fluorometric assay for pyridoxal with high specificity and sensitivity
    Yanee Trongpanich
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan
    Biosci Biotechnol Biochem 66:1152-4. 2002
    ..The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography...
  31. ncbi request reprint Purification and characterization of pyridoxal 4-dehydrogenase from Aureobacterium luteolum
    Yanee Trongpanich
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan
    Biosci Biotechnol Biochem 66:543-8. 2002
    ..The enzyme was much more tolerant to metal-chelating agents. Irreversibility of the enzymatic reaction was shared by the two enzymes. No aldehyde dehydrogenase showed similarity to the amino-terminal amino acid sequence of the enzyme...