Yasuhito Shirai

Summary

Affiliation: Kobe University
Country: Japan

Publications

  1. ncbi request reprint Diacylglycerol kinase as a possible therapeutic target for neuronal diseases
    Yasuhito Shirai
    Laboratory of Chemistry and Utilization of Animal Production Resources, Applied Chemistry in Bioscience Division, Graduate School of Agricultural Science, Kobe University, Rokkodai cho 1 1, Nada Ku, 657 8501 Kobe, Japan
    J Biomed Sci 21:28. 2014
  2. pmc A novel PIP2 binding of epsilonPKC and its contribution to the neurite induction ability
    Yasuhito Shirai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe, Japan
    J Neurochem 102:1635-44. 2007
  3. pmc The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos
    Shuji Ueda
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan
    BMC Dev Biol 13:35. 2013
  4. ncbi request reprint Protein kinase Cepsilon: function in neurons
    Yasuhito Shirai
    Biosignal Research Center, Kobe University, Kobe, Japan
    FEBS J 275:3988-94. 2008
  5. ncbi request reprint Small GTP-binding proteins in the brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori: involvement in the secretion of prothoracicotropic hormone
    Y Shirai
    Laboratory of Molecular Pharmacology, Kobe University, Hyogo, Japan
    Arch Insect Biochem Physiol 38:177-84. 1998
  6. ncbi request reprint [Analysis of molecular mechanism regulating spatio-temporal localization and activity of protein kinase C and diacylglycerol kinase using live imaging]
    Yasuhito Shirai
    Biosignal Research Center, Kobe University, Nada, Japan
    Nihon Yakurigaku Zasshi 123:189-96. 2004
  7. pmc Direct binding of RalA to PKCη and its crucial role in morphological change during keratinocyte differentiation
    Yasuhito Shirai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Mol Biol Cell 22:1340-52. 2011
  8. doi request reprint Enzymological analysis of mutant protein kinase Cgamma causing spinocerebellar ataxia type 14 and dysfunction in Ca2+ homeostasis
    Naoko Adachi
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    J Biol Chem 283:19854-63. 2008
  9. doi request reprint Ca2+ oscillation induced by P2Y2 receptor activation and its regulation by a neuron-specific subtype of PKC (gammaPKC)
    Noriaki Ashida
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Rokkodai cho 1 1, Nada Ku, Kobe 657 8501, Japan
    Neurosci Lett 446:123-8. 2008
  10. ncbi request reprint Phosphorylation and up-regulation of diacylglycerol kinase gamma via its interaction with protein kinase C gamma
    Yasuto Yamaguchi
    Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    J Biol Chem 281:31627-37. 2006

Collaborators

Detail Information

Publications49

  1. ncbi request reprint Diacylglycerol kinase as a possible therapeutic target for neuronal diseases
    Yasuhito Shirai
    Laboratory of Chemistry and Utilization of Animal Production Resources, Applied Chemistry in Bioscience Division, Graduate School of Agricultural Science, Kobe University, Rokkodai cho 1 1, Nada Ku, 657 8501 Kobe, Japan
    J Biomed Sci 21:28. 2014
    ..Here, we introduce DGKs with their structural motifs, summarize the enzymatic properties and neuronal functions, and discuss the possibility of DGKs as a therapeutic target of the neuronal diseases. ..
  2. pmc A novel PIP2 binding of epsilonPKC and its contribution to the neurite induction ability
    Yasuhito Shirai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe, Japan
    J Neurochem 102:1635-44. 2007
    ..In conclusion, epsilonPKC directly binds to PIP(2) mainly through pseudo-substrate and common region 1, contributing to the neurite induction activity...
  3. pmc The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos
    Shuji Ueda
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan
    BMC Dev Biol 13:35. 2013
    ..The isoform DGKθ has a unique domain structure and is the sole member of type V DGK. To reveal the spatial and temporal expression of DGKθ we performed immunohistochemical staining on paraffin sections of mouse embryos...
  4. ncbi request reprint Protein kinase Cepsilon: function in neurons
    Yasuhito Shirai
    Biosignal Research Center, Kobe University, Kobe, Japan
    FEBS J 275:3988-94. 2008
    ..In this review, we focus on the involvement of protein kinase Cepsilon in neurite outgrowth, presynaptic regulation, alcohol actions, ischemic preconditioning and pain...
  5. ncbi request reprint Small GTP-binding proteins in the brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori: involvement in the secretion of prothoracicotropic hormone
    Y Shirai
    Laboratory of Molecular Pharmacology, Kobe University, Hyogo, Japan
    Arch Insect Biochem Physiol 38:177-84. 1998
    ..These results indicated that the small G-proteins may possibly contribute to PTTH release in Bombyx mori...
  6. ncbi request reprint [Analysis of molecular mechanism regulating spatio-temporal localization and activity of protein kinase C and diacylglycerol kinase using live imaging]
    Yasuhito Shirai
    Biosignal Research Center, Kobe University, Nada, Japan
    Nihon Yakurigaku Zasshi 123:189-96. 2004
    ..This review summarizes diversity in targeting of PKC and DGK and the molecular mechanisms regulating their targeting...
  7. pmc Direct binding of RalA to PKCη and its crucial role in morphological change during keratinocyte differentiation
    Yasuhito Shirai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Mol Biol Cell 22:1340-52. 2011
    ..These results provide a new insight into the molecular mechanism of cytoskeletal regulation leading to drastic change of cell shape...
  8. doi request reprint Enzymological analysis of mutant protein kinase Cgamma causing spinocerebellar ataxia type 14 and dysfunction in Ca2+ homeostasis
    Naoko Adachi
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    J Biol Chem 283:19854-63. 2008
    ..Such an alteration in Ca2+ homeostasis and Ca2+-mediated signaling in Purkinje cells may contribute to the neurodegeneration characteristic of SCA14...
  9. doi request reprint Ca2+ oscillation induced by P2Y2 receptor activation and its regulation by a neuron-specific subtype of PKC (gammaPKC)
    Noriaki Ashida
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Rokkodai cho 1 1, Nada Ku, Kobe 657 8501, Japan
    Neurosci Lett 446:123-8. 2008
    ..This [Ca2]i oscillation was distinct from that induced by metabotropic glutamate receptor 5 (mGluR5) stimulation in the frequency (72.3 +/- 5.3 mHz) and in the regulatory mechanism...
  10. ncbi request reprint Phosphorylation and up-regulation of diacylglycerol kinase gamma via its interaction with protein kinase C gamma
    Yasuto Yamaguchi
    Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    J Biol Chem 281:31627-37. 2006
    ....
  11. pmc c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase α by phosphorylation at Tyr-218
    Takehiro Matsubara
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Rokkodai cho 1 1, Nada Ku, Kobe 657 8501, Japan
    J Biol Chem 287:5507-17. 2012
    ....
  12. ncbi request reprint Ceramide-induced apoptosis by translocation, phosphorylation, and activation of protein kinase Cdelta in the Golgi complex
    Taketoshi Kajimoto
    Laboratories of Molecular Pharmacology and Biochemistry, Biosignal Research Center, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    J Biol Chem 279:12668-76. 2004
    ..We demonstrate here that the targeting mechanism of PKCdelta, dual regulation of both its activation and translocation to the Golgi complex, is critical for the ceramide-induced apoptotic event...
  13. ncbi request reprint Novel variants of murine serotonin transporter mRNA and the promoter activity of its upstream site
    Kazuo Sakai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, 1 1, Rokko dai, Nada, 657 8501, Kobe, Japan
    Neurosci Lett 342:175-8. 2003
    ..These three promoter regions may play a role in the transcription of the 5-HTT and could offer a model of the regulation of 5-HTT production in humans and further the pathogenesis of depression...
  14. pmc Novel PKCα-mediated phosphorylation site(s) on cofilin and their potential role in terminating histamine release
    Megumi Sakuma
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe, Japan
    Mol Biol Cell 23:3707-21. 2012
    ....
  15. ncbi request reprint Isoform-specific membrane targeting mechanism of Rac during Fc gamma R-mediated phagocytosis: positive charge-dependent and independent targeting mechanism of Rac to the phagosome
    Takehiko Ueyama
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe, Japan
    J Immunol 175:2381-90. 2005
    ..In addition to this mechanism, Rac2 has a positive charge-independent mechanism in which Rac2 initially targets to endomembranes and then these endomembranes fuse to the phagosome...
  16. pmc A regulated adaptor function of p40phox: distinct p67phox membrane targeting by p40phox and by p47phox
    Takehiko Ueyama
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Mol Biol Cell 18:441-54. 2007
    ..Thus, both p40(phox) and p47(phox) function as diverse p67(phox) "carrier proteins" regulated by the unmasking of membrane-targeting domains in distinct mechanisms...
  17. ncbi request reprint Importance of chroman ring and tyrosine phosphorylation in the subtype-specific translocation and activation of diacylglycerol kinase alpha by D-alpha-tocopherol
    Rika Fukunaga-Takenaka
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Nadaku, Kobe 657 8501, Japan
    Genes Cells 10:311-9. 2005
    ..These results demonstrate that tyrosine phosphorylation on Tyr-334 and chroman ring are important for the D-alpha-tocopherol-induced translocation of DGKalpha...
  18. ncbi request reprint Both the C1 domain and a basic amino acid cluster at the C-terminus are important for the neurite and branch induction ability of DGKβ
    Takuya Kano
    Laboratory of Chemistry and Utilization of Animal Production Resources, Department of Applied Chemistry in Bioscience, Graduate School of Agricultural Science, Kobe University, Rokkodai cho 1 1, Nada Ku, Kobe 657 8501, Japan
    Biochem Biophys Res Commun 447:89-94. 2014
    ..These results indicate that in addition to kinase activity, plasma membrane localization via the C1 domain and basic amino acids at the C-terminus were indispensable for neurite induction by DGKβ...
  19. ncbi request reprint Activation and translocation of PKCdelta is necessary for VEGF-induced ERK activation through KDR in HEK293T cells
    Masamitsu Kuriyama
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Biochem Biophys Res Commun 325:843-51. 2004
    ..These results indicate that PKCdelta is involved in VEGF-KDR-induced ERK activation via C1B domain...
  20. ncbi request reprint Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC
    Motoi Uchino
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    J Biol Chem 279:2254-61. 2004
    ..deltaPKC, but not gammaPKC, phosphorylates mGluR5 Thr(840), leading to the blockade of both Ca2+ oscillations and gammaPKC cycling. This subtype-specific targeting proposes the molecular basis of the multiple functions of PKC...
  21. doi request reprint Suppression mechanisms of flavonoids on aryl hydrocarbon receptor-mediated signal transduction
    Rie Mukai
    Department of Agrobioscience, School of Agricultural Science, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Arch Biochem Biophys 501:134-41. 2010
    ..The inhibition of MEK/ERK phosphorylation is one of the mechanisms by which flavanones and catechins suppress the AhR-mediated signal transduction in Hepa-1c1c7 cells...
  22. ncbi request reprint Regulation of clathrin-dependent endocytosis by diacylglycerol kinase delta: importance of kinase activity and binding to AP2alpha
    Takumi Kawasaki
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Biochem J 409:471-9. 2008
    ..These results demonstrate that binding between DGKdelta2 and AP2alpha is involved in the transferrin internalization and that DGK activity is also necessary for the regulation of the endocytic process...
  23. ncbi request reprint Subtype- and species-specific knockdown of PKC using short interfering RNA
    Naoki Irie
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Japan
    Biochem Biophys Res Commun 298:738-43. 2002
    ....
  24. ncbi request reprint Nuclear transportation of diacylglycerol kinase gamma and its possible function in the nucleus
    Takehiro Matsubara
    Laboratory of Molecular Pharmacology and Laboratory of Biochemistry, Biosignal Research Center, Rokkodai cho 1 1, Nada Ku, Kobe 657 8501
    J Biol Chem 281:6152-64. 2006
    ..The cells expressing the kinase-negative mutant of DGKgamma were larger in size and showed slower growth rate, and the S phase of the cells was extended. These findings implicate that nuclear DGKgamma regulates cell cycle...
  25. ncbi request reprint Immunocytochemical localization of a neuron-specific diacylglycerol kinase beta and gamma in the developing rat brain
    Naoko Adachi
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Brain Res Mol Brain Res 139:288-99. 2005
    ..DGKgamma localized in the cytosol while DGKbeta localized along the membrane structure. These findings suggest that each DGK subtype has a spatio-temporally different function in the developmental neurons...
  26. ncbi request reprint Phospholipase A2 products retain a neuron specific gamma isoform of PKC on the plasma membrane through the C1 domain--a molecular mechanism for sustained enzyme activity
    Keiko Yagi
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Rokko dai Nada, Kobe 657 8501, Japan
    Neurochem Int 45:39-47. 2004
    ..The importance of PLA2 product and C1 domain for the retention of gammaPKC on the membrane was also confirmed using neuronal cell line, suggesting that these are part of molecular machinery for sustaining enzyme activity in neurons...
  27. ncbi request reprint Diacylglycerol kinase γ regulates antigen-induced mast cell degranulation by mediating Ca(2+) influxes
    Megumi Sakuma
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Biochem Biophys Res Commun 445:340-5. 2014
    ..These results suggest that DGKγ regulates mast cell degranulation after FcεRI cross-linking through mobilization of intracellular Ca(2+) through Ca(2+) influxes. ..
  28. doi request reprint Both C1B domain and pseudosubstrate region are necessary for saturated fatty acid-induced translocation of εPKC to the plasma membrane: distinct role of intramolecular domains for different translocation
    Takaaki Nishimoto
    The Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Rokkodai cho 1 1, Nada Ku, Kobe 657 8501, Japan
    Biochem Biophys Res Commun 432:384-8. 2013
    ..The results suggest that different domains of PKC mediate distinct translocation depending on different stimulations, contributing to their subtype- and stimulation-specific functions...
  29. pmc Essential role of neuron-enriched diacylglycerol kinase (DGK), DGKbeta in neurite spine formation, contributing to cognitive function
    Yasuhito Shirai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe, Japan
    PLoS ONE 5:e11602. 2010
    ..However, its physiological roles in neurons and higher brain function have not been elucidated...
  30. ncbi request reprint Superoxide production at phagosomal cup/phagosome through beta I protein kinase C during Fc gamma R-mediated phagocytosis in microglia
    Takehiko Ueyama
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe, Japan
    J Immunol 173:4582-9. 2004
    ..This study also suggests that DGKbeta plays a prominent role in regulation of O(2)(-) production during FcgammaR-mediated phagocytosis...
  31. ncbi request reprint The silencer activity of the novel human serotonin transporter linked polymorphic regions
    Kazuo Sakai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, 1 1 Rokko dai, Nada, Kobe 657 8501, Japan
    Neurosci Lett 327:13-6. 2002
    ..The categorized genotypic or allelic frequencies was not significantly different between the mood disorder and the control. No significant difference was detected either when analyzed by silencer activities of each allelic variant...
  32. ncbi request reprint Activation mechanisms of protein kinase C: maturation, catalytic activation, and targeting
    Yasuhito Shirai
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501
    J Biochem 132:663-8. 2002
    ..This review summarizes the evidence for the role of these three events in the isotype-specific activation of PKC, with particular emphasis on catalytic activation and targeting by lipid mediators...
  33. ncbi request reprint Importance of C1B domain for lipid messenger-induced targeting of protein kinase C
    Kaori Kashiwagi
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    J Biol Chem 277:18037-45. 2002
    ..Thus, through the C1B domain, AA and ceramide induce different patterns of epsilonPKC translocation and the C1B domain defines the subtype specific sensitivity of PKCs to lipid second messengers...
  34. ncbi request reprint Protein kinase C gamma (PKC gamma): function of neuron specific isotype
    Naoaki Saito
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657 8501
    J Biochem 132:683-7. 2002
    ..Furthermore, a point mutation in the PKC gamma gene may contribute to retinitis pigmentosa and Parkinsonian syndrome. This article reviews the specific functions of this neuron-specific isotype of PKC in neuronal signal transduction...
  35. ncbi request reprint Prothoracicotropic hormone-producing neurosecretory cells in the silkworm, Bombyx mori, express a muscarinic acetylcholine receptor
    Y Aizono
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Hyogo, Japan
    Brain Res 763:131-6. 1997
    ..These results indicated that PTTH-producing neurosecretory cells of Bombyx mori expressed an mAChR, and that muscarinic, cholinergic transmission might directly regulate PTTH release from neurosecretory cells...
  36. ncbi request reprint Inflammatory cytokine tumor necrosis factor-alpha enhances nerve growth factor production in human keratinocytes, HaCaT cells
    Koji Takaoka
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Japan
    J Pharmacol Sci 111:381-91. 2009
    ....
  37. pmc Subcellular localization of flavonol aglycone in hepatocytes visualized by confocal laser scanning fluorescence microscope
    Rie Mukai
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada Ku, Kobe, 657 8501, Japan
    Cytotechnology 59:177-82. 2009
    ..The proposed method provided evidence that flavonol aglycones are incorporated into, and accumulated in the nucleus of, hepatocytes...
  38. ncbi request reprint Distinct regulatory mechanism for p70 S6 kinase beta from that for p70 S6 kinase alpha
    T Minami
    Biosignal Research Center, Kobe University, Kobe 657 8501, Japan
    Genes Cells 6:1003-15. 2001
    ..This includes the critical phosphorylation sites, Thr252, Ser394 and Thr412 in p70alpha1, which correspond to Thr241, Ser383 and Thr401 in p70beta1, respectively. However, the regulatory mechanism for p70beta remains to be elucidated...
  39. pmc Protein kinase C-alpha mediates TNF release process in RBL-2H3 mast cells
    Ihab T Abdel-Raheem
    Department of Pharmacology, Faculty of Pharmacy, Al Azhar University, Assiut 71511, Egypt
    Br J Pharmacol 145:415-23. 2005
    ..6 These results suggest that (alpha)PKC mediates the TNF release process and azelastine inhibits TNF release by selectively interfering with the recruitment of (alpha)PKC in the pathway activated by ionomycin in RBL-2H3 cells...
  40. pmc A role for PKC-epsilon in Fc gammaR-mediated phagocytosis by RAW 264.7 cells
    Elaine C Larsen
    Centers for Cell Biology and Cancer Research, Albany Medical College, Albany, NY 12208, USA
    J Cell Biol 159:939-44. 2002
    ..These results demonstrate a role for PKC- epsilon in Fc gammaR-mediated phagocytosis that is independent of its effects on actin assembly...
  41. ncbi request reprint Propagation of gammaPKC translocation along the dendrites of Purkinje cell in gammaPKC-GFP transgenic mice
    Norio Sakai
    Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    Genes Cells 9:945-57. 2004
    ..The findings may provide us with a new insight for understanding molecular mechanisms of the synaptic plasticity including cerebellar long-term depression...
  42. ncbi request reprint Localization and functional interrelationships among cytosolic Group IV, secreted Group V, and Ca2+-independent Group VI phospholipase A2s in P388D1 macrophages using GFP/RFP constructs
    Yasuhito Shirai
    Department of Chemistry and Biochemistry, School of Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0601, USA
    Biochim Biophys Acta 1735:119-29. 2005
    ..These new data illustrate the subcellular localization changes that accompany the distinct roles that each of the three kinds of PLA(2) present in P388D(1) macrophages play in AA mobilization...
  43. ncbi request reprint Lck-dependent tyrosine phosphorylation of diacylglycerol kinase alpha regulates its membrane association in T cells
    Ernesto Merino
    Department of Immunology and Oncology, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Madrid, Spain
    J Immunol 180:5805-15. 2008
    ..The concerted action of these two signals results in transient, receptor-regulated DGKalpha relocalization to the site at which it exerts its function as a negative modulator of DAG-dependent signals...
  44. ncbi request reprint Synthesis and phorbol ester binding of the cysteine-rich domains of diacylglycerol kinase (DGK) isozymes. DGKgamma and DGKbeta are new targets of tumor-promoting phorbol esters
    Mayumi Shindo
    Applied Biosystems Japan Ltd, 4 5 4 Hacchobori, Chuo Ku, Tokyo 104 0032, Japan
    J Biol Chem 278:18448-54. 2003
    ..These results indicate that 12-O-tetradecanoylphorbol 13-acetate binds to C1A of DGKgamma to cause its translocation...
  45. ncbi request reprint [Analysis of PKC targeting mechanism using PKC fused with fluorescent proteins]
    Norio Sakai
    Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Japan
    Nihon Yakurigaku Zasshi 121:421-34. 2003
    ..We have further analyzed PKC functions in the central nervous system using transgenic mice, which can express PKC-GFP in a brain-region-specific manner...
  46. ncbi request reprint Phosphorylation of PKC activation loop plays an important role in receptor-mediated translocation of PKC
    Takahiro Seki
    Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734 8551, Japan
    Genes Cells 10:225-39. 2005
    ..Our present study suggests that the activation loop phosphorylation plays an important role in receptor-mediated PKC targeting...
  47. ncbi request reprint Localization of group V phospholipase A2 in caveolin-enriched granules in activated P388D1 macrophage-like cells
    María A Balboa
    Department of Chemistry and Biochemistry, School of Medicine, University of California at San Diego, La Jolla, California 92093 0601, USA
    J Biol Chem 278:48059-65. 2003
    ....
  48. doi request reprint Reactive oxygen species derived from NOX1/NADPH oxidase enhance inflammatory pain
    Masakazu Ibi
    Department of Pharmacology and Anatomy and Developmental Biology, Kyoto Prefectural University of Medicine, Kyoto 602 8566, Japan
    J Neurosci 28:9486-94. 2008
    ..These findings indicate that NOX1/NADPH oxidase accelerates the translocation of PKCepsilon in DRG neurons, thereby enhancing the TRPV1 activity and the sensitivity to painful stimuli...
  49. ncbi request reprint [Molecular mechanisms of PKC targeting]
    Yasuhito Shirai
    Tanpakushitsu Kakusan Koso 48:1241-7. 2003