Ken ichi Sato

Summary

Affiliation: Kobe University
Country: Japan

Publications

  1. ncbi request reprint Association of c-Src with p52Shc in mitotic NIH3T3 cells as revealed by Src-Shc binding site-specific antibodies
    Ken ichi Sato
    Research Center for Environmental Genomics, Kobe University, Nada, Kobe 657 8501, Japan
    J Biochem 137:61-7. 2005
  2. ncbi request reprint Reconstitution of Src-dependent phospholipase Cgamma phosphorylation and transient calcium release by using membrane rafts and cell-free extracts from Xenopus eggs
    Ken ichi Sato
    Research Center for Environmental Genomics, Faculty of Science, Kobe University, Nada, Kobe 657 8501, Japan
    J Biol Chem 278:38413-20. 2003
  3. ncbi request reprint Src-dependent phosphorylation of the EGF receptor Tyr-845 mediates Stat-p21waf1 pathway in A431 cells
    Ken ichi Sato
    Research Centre for Environmental Genomics, Department of Biology, Faculty of Science, Kobe University, Nada, Kobe 657 8501, Japan
    Genes Cells 8:995-1003. 2003
  4. ncbi request reprint Low density detergent-insoluble membrane of Xenopus eggs: subcellular microdomain for tyrosine kinase signaling in fertilization
    Ken ichi Sato
    Research Center for Environmental Genomics, Kobe University, Nada, Kobe 657 8501 Japan
    Development 129:885-96. 2002
  5. ncbi request reprint Hydrogen peroxide induces Src family tyrosine kinase-dependent activation of Xenopus eggs
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Dev Growth Differ 43:55-72. 2001
  6. ncbi request reprint Adaptor protein Shc undergoes translocation and mediates up-regulation of the tyrosine kinase c-Src in EGF-stimulated A431 cells
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, and Department of Biology, Faculty of Science, Kobe University, Kobe 657 8501, Japan
    Genes Cells 5:749-64. 2000
  7. ncbi request reprint Tyrosine kinase-dependent activation of phospholipase Cgamma is required for calcium transient in Xenopus egg fertilization
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Nada, Japan
    Dev Biol 224:453-69. 2000
  8. ncbi request reprint Fertilization signalling and protein-tyrosine kinases
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Nada, Japan
    Comp Biochem Physiol B Biochem Mol Biol 126:129-48. 2000
  9. ncbi request reprint Evidence for the involvement of a Src-related tyrosine kinase in Xenopus egg activation
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Kobe, 657 8501, Japan
    Dev Biol 209:308-20. 1999
  10. ncbi request reprint c-Src and phosphatidylinositol 3-kinase are involved in NGF-dependent tyrosine phosphorylation of Shc in PC12 cells
    K Sato
    Laboratory of Molecular Biology, Kobe University, Japan
    Biochem Biophys Res Commun 250:223-8. 1998

Detail Information

Publications40

  1. ncbi request reprint Association of c-Src with p52Shc in mitotic NIH3T3 cells as revealed by Src-Shc binding site-specific antibodies
    Ken ichi Sato
    Research Center for Environmental Genomics, Kobe University, Nada, Kobe 657 8501, Japan
    J Biochem 137:61-7. 2005
    ..These results support the idea that dephosphorylation-independent activation of c-Src by Shc is mediated by a molecular interaction involving the c-Src IDA region...
  2. ncbi request reprint Reconstitution of Src-dependent phospholipase Cgamma phosphorylation and transient calcium release by using membrane rafts and cell-free extracts from Xenopus eggs
    Ken ichi Sato
    Research Center for Environmental Genomics, Faculty of Science, Kobe University, Nada, Kobe 657 8501, Japan
    J Biol Chem 278:38413-20. 2003
    ..The development of such experimental platforms will allow us to dissect the molecular mechanism of sperm-dependent activation of raft-associated Src and subsequent up-regulation of PLCgamma and egg activation machinery in Xenopus eggs...
  3. ncbi request reprint Src-dependent phosphorylation of the EGF receptor Tyr-845 mediates Stat-p21waf1 pathway in A431 cells
    Ken ichi Sato
    Research Centre for Environmental Genomics, Department of Biology, Faculty of Science, Kobe University, Nada, Kobe 657 8501, Japan
    Genes Cells 8:995-1003. 2003
    ....
  4. ncbi request reprint Low density detergent-insoluble membrane of Xenopus eggs: subcellular microdomain for tyrosine kinase signaling in fertilization
    Ken ichi Sato
    Research Center for Environmental Genomics, Kobe University, Nada, Kobe 657 8501 Japan
    Development 129:885-96. 2002
    ..These findings suggest that the egg LD-DIM might serve as subcellular microdomain for tyrosine kinase signaling in Xenopus egg fertilization...
  5. ncbi request reprint Hydrogen peroxide induces Src family tyrosine kinase-dependent activation of Xenopus eggs
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Dev Growth Differ 43:55-72. 2001
    ..Together, the present study demonstrated that H2O2 can mimic, at least in part, early events of Xenopus egg activation that require an SFK-dependent PLC pathway...
  6. ncbi request reprint Adaptor protein Shc undergoes translocation and mediates up-regulation of the tyrosine kinase c-Src in EGF-stimulated A431 cells
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, and Department of Biology, Faculty of Science, Kobe University, Kobe 657 8501, Japan
    Genes Cells 5:749-64. 2000
    ..In our previous studies, Shc was shown to be a substrate of the tyrosine kinase c-Src in vitro and in vivo...
  7. ncbi request reprint Tyrosine kinase-dependent activation of phospholipase Cgamma is required for calcium transient in Xenopus egg fertilization
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Nada, Japan
    Dev Biol 224:453-69. 2000
    ..These results support the idea that Xenopus egg fertilization requires Src-family PTK-dependent PLCgamma activity that acts upstream of the calcium-dependent signaling pathway...
  8. ncbi request reprint Fertilization signalling and protein-tyrosine kinases
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Nada, Japan
    Comp Biochem Physiol B Biochem Mol Biol 126:129-48. 2000
    ..In this review, we will summarize the recent findings that begin to uncover a 'missing link' between sperm-egg interaction and egg activation with emphasis on the role of egg protein-tyrosine kinases (PTKs) in Xenopus egg fertilization...
  9. ncbi request reprint Evidence for the involvement of a Src-related tyrosine kinase in Xenopus egg activation
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Kobe, 657 8501, Japan
    Dev Biol 209:308-20. 1999
    ..Taken together, these data suggest that the tyrosine kinase Xyk plays an important role in the early events of Xenopus egg activation in a manner independent or upstream of calcium signaling...
  10. ncbi request reprint c-Src and phosphatidylinositol 3-kinase are involved in NGF-dependent tyrosine phosphorylation of Shc in PC12 cells
    K Sato
    Laboratory of Molecular Biology, Kobe University, Japan
    Biochem Biophys Res Commun 250:223-8. 1998
    ..These results suggest that coordinate action of PtdIns 3-kinase and/or PtdIns 3,4,5-trisphosphate and c-Src can function as positive regulator in tyrosine phosphorylation of Shc in vitro and in vivo...
  11. ncbi request reprint Signal transduction pathways leading to Ca2+ release in a vertebrate model system: lessons from Xenopus eggs
    Ken ichi Sato
    Laboratory of Molecular Biology, The Research Center for Environmental Genomics, Kobe University, Kobe 657 8501, Japan
    Semin Cell Dev Biol 17:285-92. 2006
    ..Finally, we address roles of egg lipid/membrane microdomains or 'rafts' as a platform for the sperm-egg membrane interaction and subsequent signaling events of egg activation...
  12. ncbi request reprint Towards the molecular dissection of fertilization signaling: Our functional genomic/proteomic strategies
    Ken ichi Sato
    Research Center for Environmental Genomics, Kobe University, Japan
    Proteomics 2:1079-89. 2002
    ....
  13. ncbi request reprint Molecular identification and characterization of Xenopus egg uroplakin III, an egg raft-associated transmembrane protein that is tyrosine-phosphorylated upon fertilization
    Keiichi Sakakibara
    Graduate School of Science and Technology, Kobe University, Nada, Japan
    J Biol Chem 280:15029-37. 2005
    ..Thus, xUPIII represents an egg raft-associated protein that is likely involved in sperm-egg interaction as well as subsequent Src-dependent intracellular events of egg activation in Xenopus...
  14. ncbi request reprint Uroplakin III, a novel Src substrate in Xenopus egg rafts, is a target for sperm protease essential for fertilization
    A K M Mahbub Hasan
    Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Dev Biol 286:483-92. 2005
    ..Our results highlight novel mechanisms of fertilization signaling by which xUPIII serves as a potential target for sperm protease essential for fertilization...
  15. ncbi request reprint Adaptor protein Shc is an isoform-specific direct activator of the tyrosine kinase c-Src
    Ken ichi Sato
    Research Center for Environmental Genomics, Department of Biology, Faculty of Science, Kobe University, Nada, Kobe 657 8501, Japan
    J Biol Chem 277:29568-76. 2002
    ..Using this mutant, we have confirmed that the Shc-mediated c-Src activation triggers Stat-p21/WAF1/Cip1 pathway that has been implicated in the cell cycle arrest and apoptosis of epidermal growth factor-stimulated A431 cells...
  16. ncbi request reprint Studying fertilization in cell-free extracts: focusing on membrane/lipid raft functions and proteomics
    Ken ichi Sato
    Research Center for Environmental Genomics, Kobe University, Japan
    Methods Mol Biol 322:395-411. 2006
    ..e., whole egg proteome) of fertilization in a cell-free manner. As one of these proteomics approaches, we provide a protocol for molecular identification of Xenopus egg raft proteins using mass spectrometry and database mining...
  17. ncbi request reprint Activation of AtMEK1, an Arabidopsis mitogen-activated protein kinase kinase, in vitro and in vivo: analysis of active mutants expressed in E. coli and generation of the active form in stress response in seedlings
    Daisuke Matsuoka
    Graduate School of Science and Technology, Department of Biological and Environmental Science, Faculty of Agriculture, Kobe University, Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Plant J 29:637-47. 2002
    ..These data indicate that the AtMEK1 becomes an active form through phosphorylation and activates its downstream target ATMPK4 in stress response in Arabidopsis...
  18. doi request reprint Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs
    Alexander A Tokmakov
    Graduate School of Science, Kobe University, Nada, Kobe, Japan
    Methods 51:177-82. 2010
    ..Herein, we describe protocols for studying molecular mechanisms of egg fertilization/activation using cell-free extracts and membrane rafts prepared from metaphase-arrested Xenopus eggs...
  19. ncbi request reprint Generation of an antibody specific to Xenopus fertilized eggs by subtractive immunization
    Keiichi Sakakibara
    Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Genes Cells 10:345-56. 2005
    ..More generally, subtractive immunization may work as a strategy to uncover cellular events that operate during different cellular conditions of interest...
  20. ncbi request reprint Expression, phosphorylation, and mRNA-binding of heterogeneous nuclear ribonucleoprotein K in Xenopus oocytes, eggs, and early embryos
    Tetsushi Iwasaki
    Research Center for Environmental Genomics, Kobe University, Kobe 657 8501, Japan
    Dev Growth Differ 50:23-40. 2008
    ..Taken together, it is attractive to suggest that hnRNP K is in association with certain pools of maternal mRNAs whose translational activation are modulated by the Src/MAPK phosphorylation of hnRNP K during oocyte-egg-embryo transition...
  21. ncbi request reprint Molecular dissection of egg fertilization signaling with the aid of tyrosine kinase-specific inhibitor and activator strategies
    Ken ichi Sato
    Research Center for Environmental Genomics, Kobe University, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan
    Biochim Biophys Acta 1697:103-21. 2004
    ..We also summarize recent advances in understanding the role of the Src family kinases in egg fertilization of other model organisms, and discuss future directions of the field...
  22. ncbi request reprint Tyrosine phosphorylation of p145met mediated by EGFR and Src is required for serum-independent survival of human bladder carcinoma cells
    Natsumi Yamamoto
    Graduate School of Science and Technology, Kobe University, Nada, Kobe, Japan
    J Cell Sci 119:4623-33. 2006
    ..These results suggest that the serum-independent growth of 5637 cells involves the transmembrane signaling cascade via EGFR ligand(s) (but not HGF), EGFR, Src and p145(met)...
  23. ncbi request reprint Characterization of Xenopus egg membrane microdomains containing uroplakin Ib/III complex: roles of their molecular interactions for subcellular localization and signal transduction
    A K M Mahbub Hasan
    Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Genes Cells 12:251-67. 2007
    ....
  24. pmc Evidence that phosphatidylinositol 3-kinase is involved in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization
    Gunay Mammadova
    The Graduate School of Science, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    BMC Dev Biol 9:68. 2009
    ..However, the roles of egg-associated PI 3-kinase and Akt, especially in signal transduction at fertilization, are not well understood...
  25. ncbi request reprint Phylogeny of vertebrate Src tyrosine kinases revealed by the epitope region of mAb327
    Tetsushi Iwasaki
    Research Center for Environmental Genomics, Kobe University, Nada, Kobe 657 8501
    J Biochem 139:347-54. 2006
    ....
  26. ncbi request reprint Inhibition and activation of c-Src: the head and tail of a coin
    Yasuo Fukami
    Department of Biology, Faculty of Science, Kobe University, Nada, 657 8501, Kobe, Japan
    Pharmacol Ther 93:263-70. 2002
    ..Our results highlight a novel and general mode of kinase regulation that may be applied not only to SFKs, but to other PTKs and Ser/Thr kinases as well...
  27. ncbi request reprint Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1-AtMEK1 pathway by wounding
    Toto Hadiarto
    Graduate School of Science and Technology, Kobe University, Rokkodai cho, Nada Ku, Japan
    Planta 223:708-13. 2006
    ..Furthermore, analysis using anti-AtMEKK1 and anti-AtMEK1 antibodies revealed that the interaction between the two proteins was signal dependent. These results suggest the presence of AtMEKK1-AtMEK1 pathway induced by wounding...
  28. ncbi request reprint Analysis of terminal sugar moieties and species-specificities of acrosome reaction-inducing substance in Xenopus (ARISX)
    Yasushi Ueda
    Research Center for Environmental Genomics, Kobe University, Kobe 657 8501, Japan
    Dev Growth Differ 49:591-601. 2007
    ..The resulting limited population of sperm from a non-Xenopus species underwent acrosome reaction, implying a weak species-specificity of ARISX...
  29. pmc 12-O-tetradecanoylphorbol-13-acetate inhibits melanoma growth by inactivation of STAT3 through protein kinase C-activated tyrosine phosphatase(s)
    Masahiro Oka
    Division of Dermatology, Department of Internal Related, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    J Biol Chem 284:30416-23. 2009
    ..Because TPA did not affect c-Src activity, we conclude that the growth inhibitory effect of TPA on melanoma cells is mediated through inactivation of STAT3 by a PKC-activated tyrosine phosphatase(s)...
  30. ncbi request reprint Inhibition of MAPK pathway by a synthetic peptide corresponding to the activation segment of MAPK
    A A Tokmakov
    Biosignal Research Center, Kobe University, Nada, Kobe, 657 8501, Japan
    Biochem Biophys Res Commun 252:214-9. 1998
    ..These results depict IDA MAPK peptide as a selective inhibitor of the MAPK pathway that can be used for the investigations of MAPK-mediated signaling...
  31. ncbi request reprint Tyrosine residues 239 and 240 of Shc are phosphatidylinositol 4,5-bisphosphate-dependent phosphorylation sites by c-Src
    K Sato
    Laboratory of Molecular Biology, Kobe University, Japan
    Biochem Biophys Res Commun 240:399-404. 1997
    ..These results highlight the difference between c-Src and v-Src or EGF receptor and suggest that c-Src can phosphorylate predominantly on Tyr239/240 of Shc only when Shc PTB domain is bound to PtdIns(4,5)P2...
  32. ncbi request reprint Involvement of protein-tyrosine phosphorylation and dephosphorylation in sperm-induced Xenopus egg activation
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Nada, Japan
    FEBS Lett 424:113-8. 1998
    ..These results suggest that both protein-tyrosine phosphorylation and dephosphorylation pathways play an important role in the sperm-induced Xenopus egg activation...
  33. ncbi request reprint Phosphatidylinositol 4,5-bisphosphate stimulates phosphorylation of the adaptor protein Shc by c-Src
    K Sato
    Laboratory of Molecular Biology, Biosignal Research Center, Kobe University, Nada, Japan
    FEBS Lett 410:136-40. 1997
    ..These results suggest that PtdIns(4,5)P2 can act as a regulator of phosphorylation of Shc by c-Src through its binding to Shc...
  34. ncbi request reprint Directional dependence of osteoblastic calcium response to mechanical stimuli
    T Adachi
    Department of Mechanical Engineering, Faculty of Engineering, Kobe University, Nada, 657 8501, Kobe, Japan
    Biomech Model Mechanobiol 2:73-82. 2003
    ....
  35. ncbi request reprint Activation of the 20S proteasome of Xenopus oocytes by cardiolipin: blockage of the activation of trypsin-like activity by the substrate
    S Yamada
    Department of Biology and Geosciences, Faculty of Science, Shizuoka University, Japan
    Biosci Biotechnol Biochem 62:1264-6. 1998
    ..These results suggest that the trypsin-type substrate produces a conformational change in the enzyme in a concentration-dependent manner which makes the activator sites inaccessible to cardiolipin...
  36. pmc Proteolytic processing of phospholipase Czeta and [Ca2+]i oscillations during mammalian fertilization
    Manabu Kurokawa
    Paige Laboratory, Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    Dev Biol 312:407-18. 2007
    ..Our results support the hypothesis that PLCzeta is the sole mammalian sperm factor and that its linker region may have important regulatory functions during mammalian fertilization...
  37. ncbi request reprint Functional, biochemical, and chromatographic characterization of the complete [Ca2+]i oscillation-inducing activity of porcine sperm
    Manabu Kurokawa
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    Dev Biol 285:376-92. 2005
    ....
  38. ncbi request reprint Mammalian fertilization: from sperm factor to phospholipase Czeta
    Manabu Kurokawa
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01373, USA
    Biol Cell 96:37-45. 2004
    ....
  39. ncbi request reprint Regulation of Src kinase activity during Xenopus oocyte maturation
    Alexander Tokmakov
    Genomic Sciences Center, RIKEN Yokohama Institute, 1 7 22 Suehiro cho, Yokohama, Kanagawa 230 0045, Japan
    Dev Biol 278:289-300. 2005
    ..Our data suggest that the Src kinase acts through the MAPK pathway to accelerate oocyte maturation...
  40. ncbi request reprint Evidence that activation of Src family kinase is not required for fertilization-associated [Ca2+]i oscillations in mouse eggs
    Manabu Kurokawa
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    Reproduction 127:441-54. 2004
    ..We can therefore conclude that activation of an SFK is neither necessary nor sufficient for triggering fertilization-induced [Ca2+]i oscillations...