H Nakayashiki

Summary

Affiliation: Kobe University
Country: Japan

Publications

  1. ncbi request reprint Degenerate MAGGY elements in a subgroup of Pyricularia grisea: a possible example of successful capture of a genetic invader by a fungal genome
    H Nakayashiki
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Japan
    Mol Gen Genet 261:958-66. 1999
  2. ncbi request reprint Evolution and diversification of RNA silencing proteins in fungi
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, 657 8501, Japan
    J Mol Evol 63:127-35. 2006
  3. doi request reprint Systematic functional analysis of calcium-signalling proteins in the genome of the rice-blast fungus, Magnaporthe oryzae, using a high-throughput RNA-silencing system
    Quoc Bao Nguyen
    Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, 1 1 Rokkodaicho, Nada, Kobe, Japan
    Mol Microbiol 68:1348-65. 2008
  4. ncbi request reprint Repeat-induced point mutation (RIP) in Magnaporthe grisea: implications for its sexual cycle in the natural field context
    Ken ichi Ikeda
    Labvoratory of Plant Pathology, Kobe University, 1 1 Rokkodaicho, Nada, 657 8501 Kobe, Japan
    Mol Microbiol 45:1355-64. 2002
  5. pmc siRNA-dependent and -independent post-transcriptional cosuppression of the LTR-retrotransposon MAGGY in the phytopathogenic fungus Magnaporthe oryzae
    Toshiki Murata
    Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Nucleic Acids Res 35:5987-94. 2007
  6. doi request reprint The Trickster in the genome: contribution and control of transposable elements
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Genes Cells 16:827-41. 2011
  7. ncbi request reprint The C-terminal chromodomain-like module in the integrase domain is crucial for high transposition efficiency of the retrotransposon MAGGY
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    FEBS Lett 579:488-92. 2005
  8. pmc Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains
    H Nakayashiki
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Nucleic Acids Res 29:4106-13. 2001
  9. ncbi request reprint RNA silencing as a tool for exploring gene function in ascomycete fungi
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodaicho, Nada, 657 8501 Kobe, Japan
    Fungal Genet Biol 42:275-83. 2005
  10. pmc Methylation is not the main force repressing the retrotransposon MAGGY in Magnaporthe grisea
    H Nakayashiki
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Kobe, 657 8501, Japan
    Nucleic Acids Res 29:1278-84. 2001

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Degenerate MAGGY elements in a subgroup of Pyricularia grisea: a possible example of successful capture of a genetic invader by a fungal genome
    H Nakayashiki
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Japan
    Mol Gen Genet 261:958-66. 1999
    ..The fact that certain members of the cluster of MAGGY carriers retain a single copy of a degenerate MAGGY element implies that the ancestor of these isolates successfully "captured" the invading MAGGY element...
  2. ncbi request reprint Evolution and diversification of RNA silencing proteins in fungi
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, 657 8501, Japan
    J Mol Evol 63:127-35. 2006
    ..Therefore, the origin of multiple RNA silencing pathways seems to be very ancient, likely having occurred prior to the divergence of the major fungal lineages...
  3. doi request reprint Systematic functional analysis of calcium-signalling proteins in the genome of the rice-blast fungus, Magnaporthe oryzae, using a high-throughput RNA-silencing system
    Quoc Bao Nguyen
    Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, 1 1 Rokkodaicho, Nada, Kobe, Japan
    Mol Microbiol 68:1348-65. 2008
    ..oryzae. These included several novel findings such as that Pmc1-, Spf1- and Neo1-like Ca(2+) pumps, calreticulin and calpactin heavy chain were essential for fungal pathogenicity...
  4. ncbi request reprint Repeat-induced point mutation (RIP) in Magnaporthe grisea: implications for its sexual cycle in the natural field context
    Ken ichi Ikeda
    Labvoratory of Plant Pathology, Kobe University, 1 1 Rokkodaicho, Nada, 657 8501 Kobe, Japan
    Mol Microbiol 45:1355-64. 2002
    ..These results imply that the sexual cycle of this fungus exists or existed in the natural field context...
  5. pmc siRNA-dependent and -independent post-transcriptional cosuppression of the LTR-retrotransposon MAGGY in the phytopathogenic fungus Magnaporthe oryzae
    Toshiki Murata
    Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Nucleic Acids Res 35:5987-94. 2007
    ..oryzae that possesses the RNA silencing machinery...
  6. doi request reprint The Trickster in the genome: contribution and control of transposable elements
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Genes Cells 16:827-41. 2011
    ..In this review, I focus on the contributions of TEs to various aspects of genome evolution. In addition, why TEs are specific targets for the genome defense mechanisms is discussed...
  7. ncbi request reprint The C-terminal chromodomain-like module in the integrase domain is crucial for high transposition efficiency of the retrotransposon MAGGY
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    FEBS Lett 579:488-92. 2005
    ..Our results indicate that the CLM may confer high transposition activity to the element...
  8. pmc Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains
    H Nakayashiki
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Nucleic Acids Res 29:4106-13. 2001
    ..This suggests that chromodomain-containing LTR retrotransposons and gammaretroviruses may share a common ancestor with the gag protein...
  9. ncbi request reprint RNA silencing as a tool for exploring gene function in ascomycete fungi
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodaicho, Nada, 657 8501 Kobe, Japan
    Fungal Genet Biol 42:275-83. 2005
    ..oryzae, indicating that this silencing vector should provide a useful reverse genetic tool in ascomycete fungi...
  10. pmc Methylation is not the main force repressing the retrotransposon MAGGY in Magnaporthe grisea
    H Nakayashiki
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Kobe, 657 8501, Japan
    Nucleic Acids Res 29:1278-84. 2001
    ..grisea with active or inactive MAGGY elements. Methylation of the MAGGY sequences was detected in some isolates but not in others. However, the methylation status did not correlate with the copy numbers and activity of the elements...
  11. ncbi request reprint RNA silencing in fungi: mechanisms and applications
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1, Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    FEBS Lett 579:5950-7. 2005
    ..Advantage and disadvantage of RNA silencing as a tool to explore gene function in fungi are discussed...
  12. doi request reprint RNA interference: roles in fungal biology
    Hitoshi Nakayashiki
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Curr Opin Microbiol 11:494-502. 2008
    ..In addition, we will briefly review recent discoveries on RNAi-related biological phenomena (RNA silencing) in fungi...
  13. ncbi request reprint Accumulation kinetics of viral gene products in cauliflower mosaic virus-infected turnip protoplasts
    K Kobayashi
    Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Japan
    Microbiol Immunol 42:65-9. 1998
    ..The results suggest that the expression of CaMV genes is differentially regulated...
  14. ncbi request reprint Comparative analyses of the distribution of various transposable elements in Pyricularia and their activity during and after the sexual cycle
    Y Eto
    Faculty of Agriculture, Kobe University, Japan
    Mol Gen Genet 264:565-77. 2001
    ..These data suggested that MAGGY was the most active of the elements tested for transposition in Pyricularia...
  15. ncbi request reprint One of the two Dicer-like proteins in the filamentous fungi Magnaporthe oryzae genome is responsible for hairpin RNA-triggered RNA silencing and related small interfering RNA accumulation
    Naoki Kadotani
    Laboratory of Plant Pathology, Faculty of Agriculture Kobe University, 1 1 Rokkodaicho, Nada, 657 8501 Kobe, Japan
    J Biol Chem 279:44467-74. 2004
    ....
  16. pmc Transposition of the retrotransposon MAGGY in heterologous species of filamentous fungi
    H Nakayashiki
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Kobe 657 8501, Japan
    Genetics 153:693-703. 1999
    ..lagenarium appeared to be rare. This is the first report that demonstrates active transposition of a fungal transposable element in heterologous hosts. Possible usage of MAGGY as a genetic tagging tool in filamentous fungi is discussed...
  17. ncbi request reprint Heat shock, copper sulfate and oxidative stress activate the retrotransposon MAGGY resident in the plant pathogenic fungus Magnaporthe grisea
    K Ikeda
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Nada, Japan
    Mol Genet Genomics 266:318-25. 2001
    ..The relationship between the activation of MAGGY by stress and phenotypic diversification in M. grisea, including variations in pathogenicity, is discussed...
  18. ncbi request reprint OARE-1, a Ty1-copia retrotransposon in oat activated by abiotic and biotic stresses
    Y Kimura
    Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, Rokkodai-cho, Nada-ku, Kobe, 657-8501 Japan
    Plant Cell Physiol 42:1345-54. 2001
    ..These results suggest that OARE-1 is highly sensitive to various abiotic and biotic stimuli leading to plant defense responses...
  19. ncbi request reprint Induction and signaling of an apoptotic response typified by DNA laddering in the defense response of oats to infection and elicitors
    Y Tada
    Graduate School of Natural Science and Technology, Kobe University, Japan
    Mol Plant Microbe Interact 14:477-86. 2001
    ..The results also demonstrated that the mediators involved in the induction of apoptosis depended on the type of stimuli, whereas p28 and serine and cysteine proteases commonly are associated with each elicitor-induced apoptosis...
  20. ncbi request reprint Evidence for a dual strategy in the expression of cauliflower mosaic virus open reading frames I and IV
    K Kobayashi
    Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Japan
    Microbiol Immunol 42:329-34. 1998
    ..These results suggest the involvement of subgenomic RNAs in the expression of CaMV ORFs I and IV, and the operation of a dual strategy in the expression of two viral genes...
  21. ncbi request reprint Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae
    I Chuma
    Faculty of Agriculture, Kobe University, 657 8501 Kobe, Japan
    Curr Genet 43:191-8. 2003
    ..An ectopic sister chromatid recombination may cause the size variation of the minichromosomes...
  22. ncbi request reprint Novel evidence for apoptotic cell response and differential signals in chromatin condensation and DNA cleavage in victorin-treated oats
    N Yao
    Laboratory of Plant Pathology, The Graduate School of Science and Technology, Kobe University, Rokkodai, Nada-ku, Kobe 657-8501, Japan
    Plant J 28:13-26. 2001
    ..Moreover, protein kinases were involved in chromatin condensation, but did not affect DNA digestion, suggesting that chromatin condensation and DNA cleavage are differentially regulated in the death process in oats...
  23. ncbi request reprint RNA silencing in the phytopathogenic fungus Magnaporthe oryzae
    Naoki Kadotani
    Laboratory of Plant Pathology, Kobe University, 1 1 Rokkodaicho, Nada, 657 8501 Kobe, Japan
    Mol Plant Microbe Interact 16:769-76. 2003
    ..Overall, these results indicate that RNA silencing operates in M. oryzae, which gives us a new tool for genome-wide gene analysis in this fungus...
  24. doi request reprint Evolution of the Eleusine subgroup of Pyricularia oryzae inferred from rearrangement at the Pwl1 locus
    Masaki Tanaka
    Laboratory of Plant Pathology, Graduate School of Agricultural Sciences, Kobe University, Nada, Kobe 657 8501, Japan
    Mol Plant Microbe Interact 23:771-83. 2010
    ..Based on these results, a model was constructed which illustrated possible pathways to the establishment of the Eleusine subgroup...
  25. ncbi request reprint Evolution of an avirulence gene, AVR1-CO39, concomitant with the evolution and differentiation of Magnaporthe oryzae
    Yukio Tosa
    Faculty of Agriculture, Graduate School of Science and Technology, Kobe University, Nada, Japan
    Mol Plant Microbe Interact 18:1148-60. 2005
    ..These results indicate that AVR1-CO39 was lost during the early stage of evolution of the Oryza-specific subgroup of P. oryzae. Interestingly, its corresponding resistance gene, Pi-CO39(t), is not widely distributed in Oryza spp...
  26. ncbi request reprint Speciation in Pyricularia inferred from multilocus phylogenetic analysis
    Kenji Hirata
    Graduate School of Agriculture, Kobe University, Nada, Kobe 657 8501, Japan
    Mycol Res 111:799-808. 2007
    ..They were interpreted by assuming that Pyricularia has evolved through repeated species radiation. The transition point other than the current species limit was considered to be the limit of the former species...
  27. ncbi request reprint Requirement of cauliflower mosaic virus open reading frame VI product for viral gene expression and multiplication in turnip protoplasts
    K Kobayashi
    Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University, Japan
    Microbiol Immunol 42:377-86. 1998
    ..These results suggest that P6 is involved in the stabilization of other viral gene products as well as in the activation of viral gene expression, and thus, is a prerequisite for CaMV multiplication...
  28. ncbi request reprint Apoptotic cell death is a common response to pathogen attack in oats
    Nan Yao
    Laboratory of Plant Pathology, The Graduate School of Science and Technology, Kobe University, Rokkodai, Japan
    Mol Plant Microbe Interact 15:1000-7. 2002
    ..Possible roles of apoptotic cell death in the susceptible and resistant interactions are discussed...
  29. pmc Transcriptional control and protein specialization have roles in the functional diversification of two dicer-like proteins in Magnaporthe oryzae
    Naoki Kadotani
    Laboratory of Plant Pathology, Kobe University, Kobe 657 8501, Japan
    Genetics 180:1245-9. 2008
    ....
  30. ncbi request reprint Nitric oxide and reactive oxygen species do not elicit hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oat
    Yasuomi Tada
    Graduate School of Natural Science and Technology, Kobe University, Kobe 657 8501, Japan
    Mol Plant Microbe Interact 17:245-53. 2004
    ..However, NO and O2- but not H2O2 are required for the onset of apoptotic cell death in the adjacent cells, where excess NO may exert its anti-apoptotic function by regulating cellular redox state...
  31. ncbi request reprint Specific cleavage of ribosomal RNA and mRNA during victorin-induced apoptotic cell death in oat
    Trinh X Hoat
    Laboratory of Plant Pathology, Graduate School of Science and Technology, Kobe University, Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Plant J 46:922-33. 2006
    ..The RNA degradation mostly, but not always, occurred in parallel with DNA laddering, but pharmacological studies indicated that these processes are regulated by different signaling pathways with some overlapping upstream signals...
  32. ncbi request reprint Microarray analysis of the gene expression profile induced by the endophytic plant growth-promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis
    Yanqing Wang
    Laboratory of Plant Pathology, Graduate School of Science and Technology, Kobe University, Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Mol Plant Microbe Interact 18:385-96. 2005
    ..Our results suggest that P. fluorescens FPT9601-T5 triggered plant responses in a manner similar to known PGPR and, at least in some aspects, to rhizobia...
  33. ncbi request reprint Mitochondrial oxidative burst involved in apoptotic response in oats
    Nan Yao
    Laboratory of Plant Pathology, Graduate School of Science and Technology, Kobe University, Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Plant J 30:567-79. 2002
    ..These findings indicate possible involvement of mitochondria, especially mitochondrial-derived ROS generation, as an important regulator in controlling apoptotic cell death in oats...
  34. ncbi request reprint Analysis of the involvement of hydroxyanthranilate hydroxycinnamoyltransferase and caffeoyl-CoA 3-O-methyltransferase in phytoalexin biosynthesis in oat
    Qian Yang
    Laboratory of Plant Pathology, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Mol Plant Microbe Interact 17:81-9. 2004
    ....
  35. ncbi request reprint Analysis of the structure of the AVR1-CO39 avirulence locus in virulent rice-infecting isolates of Magnaporthe grisea
    Mark L Farman
    Department of Plant Pathology, University of Wisconsin, Madison 53706, USA
    Mol Plant Microbe Interact 15:6-16. 2002
    ..These findings suggest that widespread virulence to 'CO39' among rice-infecting M. grisea isolates is due to ancestral rearrangements at the AVR1-CO39 locus that may have occurred early in the evolution of pathogenicity to rice...
  36. ncbi request reprint Induction of apoptotic cell death leads to the development of bacterial rot caused by Pseudomonas cichorii
    Akinori Kiba
    Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University, Nankoku, 783 8502 Japan
    Mol Plant Microbe Interact 19:112-22. 2006
    ..These results suggest that apoptotic cell death might be closely associated with the development of bacterial rot caused by P. cichorii...