Y Mizushina


Affiliation: Kobe Gakuin University
Country: Japan


  1. Mizushina Y, Kuriyama I, Yamazaki A, Akashi T, Yoshida H. Cycloartenyl trans-ferulate, a component of the bran byproduct of sake-brewing rice, inhibits mammalian DNA polymerase and suppresses inflammation. Food Chem. 2013;141:1000-7 pubmed publisher
    ..These results suggest that this byproduct formed during the sake-brewing process is useful as an anti-inflammatory agent. ..
  2. Mizushina Y, Kuriyama I, Nakahara T, Kawashima Y, Yoshida H. Inhibitory effects of ?-mangostin on mammalian DNA polymerase, topoisomerase, and human cancer cell proliferation. Food Chem Toxicol. 2013;59:793-800 pubmed publisher
    ..These results suggest that decreased proliferation by ?-mangostin may be a result of the inhibition of cellular topos rather than pols, and ?-mangostin might be an anticancer chemotherapeutic agent. ..
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    Mizushina Y, Kasai N, Iijima H, Sugawara F, Yoshida H, Sakaguchi K. Sulfo-quinovosyl-acyl-glycerol (SQAG), a eukaryotic DNA polymerase inhibitor and anti-cancer agent. Curr Med Chem Anticancer Agents. 2005;5:613-25 pubmed
    ..Based on these results, the molecular actions of SQAGs and drug design strategies for developing new anti-neoplastic agents were discussed. ..
  4. Mizushina Y, Motoshima H, Yamaguchi Y, Takeuchi T, Hirano K, Sugawara F, et al. 3-O-methylfunicone, a selective inhibitor of mammalian Y-family DNA polymerases from an Australian sea salt fungal strain. Mar Drugs. 2009;7:624-39 pubmed publisher
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    Mizushina Y, Akihisa T, Ukiya M, Hamasaki Y, Murakami Nakai C, Kuriyama I, et al. Structural analysis of isosteviol and related compounds as DNA polymerase and DNA topoisomerase inhibitors. Life Sci. 2005;77:2127-40 pubmed
    ..0%). The relationship between the structure of stevioside-based compounds and these activities were discussed. ..
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    Mizushina Y, Tsuzuki T, Eitsuka T, Miyazawa T, Kobayashi K, Ikawa H, et al. Inhibitory action of conjugated C18-fatty acids on DNA polymerases and DNA topoisomerases. Lipids. 2004;39:977-83 pubmed
    ..The 3-D structure of the active site of both DNA polymerases and topoisomerases must have had a pocket to join alpha-eleostearic acid, and this pocket was 12.03 A long and 9.24 A wide. ..
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    Mizushina Y, Murakami C, Takikawa H, Kasai N, Xu X, Mori K, et al. Molecular action mode of Hippospongic acid A, an inhibitor of gastrulation of starfish embryos. J Biochem. 2003;133:541-52 pubmed
    ..We also discussed the in vivo role of HA-A. ..
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    Mizushina Y, Kasai N, Sugawara F, Sakaguchi K. [Structural homology between molecular probes binding site of DNA polymerase beta and DNA topoisomerase II]. Tanpakushitsu Kakusan Koso. 2002;47:708-14 pubmed
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    Mizushina Y, Takeuchi T, Takakusagi Y, Yonezawa Y, Mizuno T, Yanagi K, et al. Coenzyme Q10 as a potent compound that inhibits Cdt1-geminin interaction. Biochim Biophys Acta. 2008;1780:203-13 pubmed
    ..The application of inhibitors for the formation of Cdt1-geminin complex is discussed. ..

More Information


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    Mizushina Y, Takeuchi T, Takakusagi Y, Sugawara F, Sakaguchi K, Yoshida H, et al. Inhibitory action of polyunsaturated fatty acids on Cdt1-geminin interaction. Int J Mol Med. 2008;21:281-90 pubmed
    ..These results suggested that, at least in vitro, oleic acid-containing cell membranes of the lipid bilayer inhibit Cdt1-geminin complex formation by binding to Cdt1 and thereby liberating Cdt1 from inhibition by geminin. ..
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    Mizushina Y. Specific inhibitors of mammalian DNA polymerase species. Biosci Biotechnol Biochem. 2009;73:1239-51 pubmed
    ..These compounds might be useful not only as "molecular probes" for pol research, but also as biomedical and chemotherapeutic drugs for anti-cancer or anti-inflammation. ..
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    Mizushina Y, Xu X, Asano N, Kasai N, Kato A, Takemura M, et al. The inhibitory action of pyrrolidine alkaloid, 1,4-dideoxy-1,4-imino-D-ribitol, on eukaryotic DNA polymerases. Biochem Biophys Res Commun. 2003;304:78-85 pubmed
    ..The relationship between the structure of DRB and the inhibition of eukaryotic DNA polymerases is discussed. ..
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    Mizushina Y, Kamisuki S, Kasai N, Ishidoh T, Shimazaki N, Takemura M, et al. Petasiphenol: a DNA polymerase lambda inhibitor. Biochemistry. 2002;41:14463-71 pubmed
    ..On the basis of these results, the pol lambda inhibitory mechanism of petasiphenol is discussed. ..
  5. Mizushina Y, Manita D, Takeuchi T, Sugawara F, Kumamoto Yonezawa Y, Matsui Y, et al. The inhibitory action of kohamaic acid A derivatives on mammalian DNA polymerase beta. Molecules. 2008;14:102-21 pubmed publisher
    ..The relationship between the three-dimensional molecular structures of KA-A-related compounds and these inhibitory activities is discussed. ..
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    Mizushina Y, Akihisa T, Ukiya M, Murakami C, Kuriyama I, Xu X, et al. A novel DNA topoisomerase inhibitor: dehydroebriconic acid, one of the lanostane-type triterpene acids from Poria cocos. Cancer Sci. 2004;95:354-60 pubmed
    ..6 and 38.4 microM, respectively. The cells were halted at the G1 phase in the cell cycle. The relation between the structure of triterpene acids and their inhibitory activities is discussed. ..
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    Mizushina Y, Kasai N, Miura K, Hanashima S, Takemura M, Yoshida H, et al. Structural relationship of lithocholic acid derivatives binding to the N-terminal 8-kDa domain of DNA polymerase beta. Biochemistry. 2004;43:10669-77 pubmed
    ..From these results, the structure-function relationship among pol beta and its selective inhibitors was discussed. ..
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    Mizushina Y, Murakami C, Yogi K, Ueda K, Ishidoh T, Takemura M, et al. Kohamaic acid A, a novel sesterterpenic acid, inhibits activities of DNA polymerases from deuterostomes. Biochim Biophys Acta. 2003;1648:55-61 pubmed
    ..beta. KA-A is an inhibitor of replicative DNA polymerases from the deuterostome species, and subsequently, the inhibition of the first cleavage of fertilized sea urchin eggs might occur as a result of the suppression of DNA replication. ..
  9. Mizushina Y, Takeuchi T, Hada T, Maeda N, Sugawara F, Yoshida H, et al. The inhibitory action of SQDG (sulfoquinovosyl diacylglycerol) from spinach on Cdt1-geminin interaction. Biochimie. 2008;90:947-56 pubmed publisher
    ..These data could help to further understanding of the structure and function of Cdt1. In addition, SQDG could be a clue to developing more appropriate inhibitors of Cdt1-geminin interactions. ..
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    Mizushina Y, Ishidoh T, Kamisuki S, Nakazawa S, Takemura M, Sugawara F, et al. Flavonoid glycoside: a new inhibitor of eukaryotic DNA polymerase alpha and a new carrier for inhibitor-affinity chromatography. Biochem Biophys Res Commun. 2003;301:480-7 pubmed
    ..We then made a KAG-conjugated column such as the epoxy-activated Sepharose 6B. In the column, pol alpha was selectively adsorbed and eluted. ..
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    Mizushina Y, Dairaku I, Yanaka N, Takeuchi T, Ishimaru C, Sugawara F, et al. Inhibitory action of polyunsaturated fatty acids on IMP dehydrogenase. Biochimie. 2007;89:581-90 pubmed
    ..Based on these results, the IMPDH-inhibitory mechanism of fatty acids is discussed. ..