Akihiko Kondo

Summary

Affiliation: Kobe University
Country: Japan

Publications

  1. pmc Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes
    Yuya Nishimura
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    J Nanobiotechnology 11:19. 2013
  2. pmc A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway
    Yuya Nishimura
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    J Nanobiotechnology 12:11. 2014
  3. pmc Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module
    Soichiro Tabuchi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 87:1783-9. 2010
  4. pmc Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose
    Ryosuke Yamada
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Microb Cell Fact 13:71. 2014
  5. pmc Cell wall trapping of autocrine peptides for human G-protein-coupled receptors on the yeast cell surface
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, Japan
    PLoS ONE 7:e37136. 2012
  6. pmc Bright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cells
    Yasuyuki Nakamura
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan
    PLoS ONE 8:e82237. 2013
  7. pmc Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance
    Fumio Matsuda
    RIKEN Center for Sustainable Resource Science, 1 7 22 Suehirocho, Turumi ku, Yokohama, Kanagawa 230 0045, Japan
    Microb Cell Fact 12:119. 2013
  8. pmc Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans
    Takaya Miyazaki
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Microb Cell Fact 12:88. 2013
  9. pmc Benzoic acid fermentation from starch and cellulose via a plant-like β-oxidation pathway in Streptomyces maritimus
    Shuhei Noda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Microb Cell Fact 11:49. 2012
  10. pmc Biogenic synthesis and characterization of gold nanoparticles by Escherichia coli K12 and its heterogeneous catalysis in degradation of 4-nitrophenol
    Sarvesh Kumar Srivastava
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, 657 8501, Nada, Kobe, Japan
    Nanoscale Res Lett 8:70. 2013

Collaborators

Detail Information

Publications102 found, 100 shown here

  1. pmc Targeting cancer cell-specific RNA interference by siRNA delivery using a complex carrier of affibody-displaying bio-nanocapsules and liposomes
    Yuya Nishimura
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    J Nanobiotechnology 11:19. 2013
    ..However, since siRNA is unstable in blood and unable to cross the cell membrane, encapsulation of siRNA into a carrier is required...
  2. pmc A display of pH-sensitive fusogenic GALA peptide facilitates endosomal escape from a Bio-nanocapsule via an endocytic uptake pathway
    Yuya Nishimura
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    J Nanobiotechnology 12:11. 2014
    ..To assist the endosomal escape of inclusions, a helper lipid with pH-sensitive fusogenic ability (1,2-dioleoyl-sn-glycero-3-phos phoethanolamine; DOPE) was conjugated with a ZHER2-BNC...
  3. pmc Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module
    Soichiro Tabuchi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 87:1783-9. 2010
    ..Together, these results show that CBM used as a first anchor protein enables the fusion of both the N and/or C terminus of a target protein...
  4. pmc Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose
    Ryosuke Yamada
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Microb Cell Fact 13:71. 2014
    ..Currently, kojic acid is produced from glucose. The efficient production of kojic acid using cellulose as an inexpensive substrate would help establish cost-effective kojic acid production...
  5. pmc Cell wall trapping of autocrine peptides for human G-protein-coupled receptors on the yeast cell surface
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, Japan
    PLoS ONE 7:e37136. 2012
    ..Our strategy may be applied to identify eligible peptides with agonistic activity for target human GPCRs...
  6. pmc Bright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cells
    Yasuyuki Nakamura
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan
    PLoS ONE 8:e82237. 2013
    ..Our system could be useful for identifying lead peptides with agonistic activity towards targeted human GPCR(s). ..
  7. pmc Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance
    Fumio Matsuda
    RIKEN Center for Sustainable Resource Science, 1 7 22 Suehirocho, Turumi ku, Yokohama, Kanagawa 230 0045, Japan
    Microb Cell Fact 12:119. 2013
    ..However, the isobutanol yields and titers attained by the developed strains need to be further improved through engineering of S. cerevisiae metabolism...
  8. pmc Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans
    Takaya Miyazaki
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Microb Cell Fact 12:88. 2013
    ..In this study, we focused on Streptomyces lividans as a host strain to secrete useful proteins, and screened for signal peptides from the biomass-degradation enzymes derived from Thermobifida fusca YX and S. lividans...
  9. pmc Benzoic acid fermentation from starch and cellulose via a plant-like β-oxidation pathway in Streptomyces maritimus
    Shuhei Noda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Microb Cell Fact 11:49. 2012
    ..In this study, we carried out benzoate fermentation using S. maritimus. In order to enhance benzoate productivity using cellulose as the carbon source, we constructed endo-glucanase secreting S. maritimus...
  10. pmc Biogenic synthesis and characterization of gold nanoparticles by Escherichia coli K12 and its heterogeneous catalysis in degradation of 4-nitrophenol
    Sarvesh Kumar Srivastava
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, 657 8501, Nada, Kobe, Japan
    Nanoscale Res Lett 8:70. 2013
    ..This is the first report on bacterial membrane-Au NP nanobiocomposite serving as an efficient heterogeneous catalyst in complete reduction of nitroaromatic pollutant in water...
  11. doi request reprint Development of microbial cell factories for bio-refinery through synthetic bioengineering
    Akihiko Kondo
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    J Biotechnol 163:204-16. 2013
    ....
  12. pmc Engineering strategy of yeast metabolism for higher alcohol production
    Fumio Matsuda
    Organization of Advanced Science and Technology, Kobe University, Rokkodaicho, Nada Ku, Kobe, Hyogo, Japan
    Microb Cell Fact 10:70. 2011
    ..Based on the simulation results, novel metabolic engineering strategies for improving higher alcohol production by S. cerevisiae were investigated by in silico modifications of the metabolic models of S. cerevisiae...
  13. ncbi request reprint Applications of yeast cell-surface display in bio-refinery
    Akihiko Kondo
    Graduate School of Engineering, Kobe University, Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Recent Pat Biotechnol 4:226-34. 2010
    ..This review gives an insight in to the recent technological developments in the production of bioenergy, i.e, bioethanol using surface engineered yeast...
  14. pmc Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression
    Ryosuke Yamada
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Biotechnol Biofuels 4:8. 2011
    ..abstract:..
  15. doi request reprint An integrative process model of enzymatic biodiesel production through ethanol fermentation of brown rice followed by lipase-catalyzed ethanolysis in a water-containing system
    Daisuke Adachi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Enzyme Microb Technol 52:118-22. 2013
    ..The developed process model, therefore, would contribute to biodiesel production from only biomass-derived feedstocks...
  16. doi request reprint D-lactic acid production from cellooligosaccharides and beta-glucan using L-LDH gene-deficient and endoglucanase-secreting Lactobacillus plantarum
    Kenji Okano
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Japan
    Appl Microbiol Biotechnol 85:643-50. 2010
    ..47 g/l of D: -lactic was produced with a high optical purity of 99.7%. This is the first report of direct lactic acid fermentation from beta-glucan and a cellooligosaccharide that is a more highly polymerized sugar than cellotriose...
  17. doi request reprint An energy-saving glutathione production method from low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae
    Kiyotaka Y Hara
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Japan
    Biotechnol J 7:686-9. 2012
    ..cerevisiae. The combination of the amylase-expressing yeast with low-temperature cooking is potentially applicable to a variety of energy-saving bio-production methods of chemicals from starchy bio-resources...
  18. doi request reprint Novel strategy for yeast construction using delta-integration and cell fusion to efficiently produce ethanol from raw starch
    Ryosuke Yamada
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 85:1491-8. 2010
    ..Our strategy for constructing yeasts resulted in the simultaneous overexpression of genes integrated into the genome and improvements in the practical potential of yeasts...
  19. doi request reprint Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B
    Daisuke Adachi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Kobe 657 8501, Japan
    Bioresour Technol 135:410-6. 2013
    ..Therefore, the presented reaction system using r-CALB shows promise for biodiesel production from plant oil hydrolysates...
  20. doi request reprint Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells
    Takatoshi Sakamoto
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan
    J Biotechnol 158:203-10. 2012
    ..41g/g, which corresponded to 82% of the theoretical yield. The cell surface-engineered strain was thus highly effective for consolidating the process of ethanol production from hemicellulosic materials...
  21. pmc Regulation of the display ratio of enzymes on the Saccharomyces cerevisiae cell surface by the immunoglobulin G and cellulosomal enzyme binding domains
    Junji Ito
    Department of Chemical Science and Engineering, Kobe University, Rokkodaicho, Nada Ku, Japan
    Appl Environ Microbiol 75:4149-54. 2009
    ..These results strongly indicated that the proposed strategy, the simultaneous display of two enzymes on the yeast cell surface, was accomplished by quantitatively controlling the display system using affinity binding...
  22. doi request reprint Enzyme-mediated site-specific antibody-protein modification using a ZZ domain as a linker
    Takayuki Sakamoto
    Department of Chemical Science and Engineering, Graduate School of Engineering, and Organization of Advanced Science and Technology, Kobe University, Nada, Kobe 657 8501, Japan
    Bioconjug Chem 21:2227-33. 2010
    ..These results suggest that the enzymatic approach with SrtA facilitates the construction of ZZ-proteins. Furthermore, mixing antibody and ZZ-proteins produces a wide variety of antibody-ZZ-proteins...
  23. ncbi request reprint Construction of an Aspergillus oryzae cell-surface display system using a putative GPI-anchored protein
    Takashi Adachi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo, 657 8501, Japan
    Appl Microbiol Biotechnol 81:711-9. 2008
    ..Based on these results, it was concluded that the MP1 protein can act as a GPI-anchor protein in A. oryzae, and the proposed cell-surface display system using MP1 allows for the display of heterogeneous and endogenous proteins...
  24. doi request reprint Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803
    Ancy Joseph
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    FEBS Lett 588:466-71. 2014
    ..Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation. ..
  25. doi request reprint Yeast-based fluorescence reporter assay of G protein-coupled receptor signalling for flow cytometric screening: FAR1-disruption recovers loss of episomal plasmid caused by signalling in yeast
    Jun Ishii
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Graduate School of Engineering, Kobe University, Japan
    J Biochem 143:667-74. 2008
    ..Our system will be a powerful tool for the quantitative and high-throughput GPCR screening of yeast-based combinatorial libraries using FCM...
  26. doi request reprint Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:2597-607. 2013
    ....
  27. doi request reprint Development of a glutathione production process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae
    Kiyotaka Y Hara
    Organization of Advanced Science and Technology, Kobe University, Nada Ku, Kobe, Japan
    Appl Microbiol Biotechnol 93:1495-502. 2012
    ..This protease-displaying yeast is potentially applicable to a variety of processes for the bio-production of value-added chemicals from proteinaceous biomass resources...
  28. pmc Efficient production of optically pure D-lactic acid from raw corn starch by using a genetically modified L-lactate dehydrogenase gene-deficient and alpha-amylase-secreting Lactobacillus plantarum strain
    Kenji Okano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Appl Environ Microbiol 75:462-7. 2009
    ..Using this strain, direct D-lactic acid fermentation from raw corn starch was accomplished in the absence of selective pressure by antibiotics. This is the first report of direct D-lactic acid fermentation from raw starch...
  29. doi request reprint Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes
    Shuhei Yanase
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 88:381-8. 2010
    ..2% of the theoretical yield. This indicates that high-temperature cellulose fermentation to ethanol can be efficiently accomplished using a recombinant K. marxianus strain displaying thermostable cellulolytic enzymes on the cell surface...
  30. ncbi request reprint Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe, 657 8501, Japan
    J Ind Microbiol Biotechnol 40:1039-50. 2013
    ..We also speculate that the strong relationship between high temperature and increased xylitol accumulation represents the cell's mechanism to protect itself from heat degradation...
  31. ncbi request reprint Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from Candida antarctica
    Sriappareddy Tamalampudi
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 75:387-95. 2007
    ....
  32. ncbi request reprint Direct production of L-lysine from raw corn starch by Corynebacterium glutamicum secreting Streptococcus bovis alpha-amylase using cspB promoter and signal sequence
    Toshihiro Tateno
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 77:533-41. 2007
    ..These results indicate that efficient simultaneous saccharification and fermentation of raw corn starch to L-lysine were achieved by C. glutamicum secreting AmyA using the cspB promoter and signal sequence...
  33. doi request reprint Improved homo L-lactic acid fermentation from xylose by abolishment of the phosphoketolase pathway and enhancement of the pentose phosphate pathway in genetically modified xylose-assimilating Lactococcus lactis
    Satoru Shinkawa
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 91:1537-44. 2011
    ..1 g/l of L-lactic acid from xylose with a high optical purity of 99.6% and a high yield of 1.58 (moles per mole xylose consumed) that is close to theoretical value of 1.67 from xylose...
  34. doi request reprint Rapid and efficient selection of yeast displaying a target protein using thermo-responsive magnetic nanoparticles
    Nobuo Fukuda
    Department of Chemical Science and Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Biotechnol Prog 24:352-7. 2008
    ..001% to nearly 100%. Our results suggest that magnetic separation will be useful for efficient exploration of novel protein-protein interactions and rapid isolation of biomolecules with novel functions...
  35. doi request reprint Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan Electronic address
    J Biosci Bioeng 117:165-9. 2014
    ..The co-expression of TAL/ADH genes is one crucial strategy to fully utilize undetoxified lignocellulosic hydrolysate, leading to cost-competitive ethanol production. ..
  36. ncbi request reprint Quantitative and dynamic analyses of G protein-coupled receptor signaling in yeast using Fus1, enhanced green fluorescence protein (EGFP), and His3 fusion protein
    Jun Ishii
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, Nada Ku, Japan
    Biotechnol Prog 22:954-60. 2006
    ..This method will be applicable to other comprehensive GPCR ligand screening methods...
  37. doi request reprint Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae
    Takashi Kondo
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, Japan
    J Biotechnol 159:32-7. 2012
    ..cerevisiae was elevated 13-fold, from 11 mg/l to 143 mg/l, and the yield was 6.6 mg/g glucose, which is higher than any previously reported value for S. cerevisiae...
  38. doi request reprint Granting specificity for breast cancer cells using a hepatitis B core particle with a HER2-targeted affibody molecule
    Yuya Nishimura
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    J Biochem 153:251-6. 2013
    ..These results show that the display of other types of affibody molecules on HBc particles would be an expandable strategy for targeting several kinds of cancer cells that would help enable a pinpoint DDS...
  39. ncbi request reprint Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilating yeast strain
    Satoshi Katahira
    Division of Molecular Science, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 72:1136-43. 2006
    ..These results demonstrate that the fermentation of the lignocellulose hydrolysate is performed efficiently by the recombinant Saccharomyces strain with abilities for xylose assimilation and cellooligosaccharide degradation...
  40. doi request reprint Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 90:997-1004. 2011
    ..The fermentation profile also indicated that the production of xylitol and glycerol, major by-products in xylose fermentation, was not affected by the upregulation of FDH activity...
  41. doi request reprint Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli
    Chihiro Takahashi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Enzyme Microb Technol 51:171-6. 2012
    ..37 ± 0.88 g/L after 72 h with a space-time yield of 0.172 g/L/h, which is the highest yield obtained to date for GABA from fermentation with glucose as a main carbon source...
  42. doi request reprint Direct and efficient ethanol production from high-yielding rice using a Saccharomyces cerevisiae strain that express amylases
    Ryosuke Yamada
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Enzyme Microb Technol 48:393-6. 2011
    ..This is first report of direct ethanol production on real biomass using an amylase-expressing yeast strain without any pretreatment or commercial enzyme addition...
  43. doi request reprint Water activity dependence of performance of surface-displayed lipase in yeast cells: a unique water requirement for enzymatic synthetic reaction in organic media
    Ayumi Yoshida
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Enzyme Microb Technol 48:334-8. 2011
    ..These results indicate that lipases displayed on yeast cells show a unique a(w) dependence probably because of the variation in cell surface characteristics...
  44. doi request reprint Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits
    Kenji Okano
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Japan
    Appl Microbiol Biotechnol 85:413-23. 2010
    ..In this review, the drawbacks as well as improvements of LA production by fermentation is discussed...
  45. ncbi request reprint Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering
    Seiji Shibasaki
    Department of Applied Chemistry, Kobe City College of Technology, Gakuenhigashi, Nishi ku, Kobe, 651 2194, Japan
    Appl Microbiol Biotechnol 75:821-8. 2007
    ..These results suggest that the ZZ-domain-displaying cell and Fc fusion protein-secreting cell can be applied to use in synergistic process of production and recovery of secreted recombinant proteins...
  46. doi request reprint Gγ recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions
    Nobuo Fukuda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada Ku, Kobe, Japan
    FEBS J 278:3086-94. 2011
    ..This new approach will be useful for exploring the numerous transient interactions that remain undefined because of the lack of powerful screening tools for their identification...
  47. ncbi request reprint High-efficiency recovery of target cells using improved yeast display system for detection of protein-protein interactions
    Nobuo Fukuda
    Department of Chemical Science and Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 76:151-8. 2007
    ..These results indicate that we can achieve a rapid and highly efficient isolation method for the target cells with FACSCalibur and that this method will further extend the application of flow cytometric sorting to library selections...
  48. doi request reprint A simple and immediate method for simultaneously evaluating expression level and plasmid maintenance in yeast
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, Japan
    J Biochem 145:701-8. 2009
    ..We show that the FCM analysis with GFP reporter is a suitable method to explore the hopeful expression vector and host strain or establish the several expression systems exhibiting the characteristic properties in yeast...
  49. ncbi request reprint Specific protein delivery to target cells by antibody-displaying bionanocapsules
    Naoya Kurata
    Department of Chemical Science and Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    J Biochem 144:701-7. 2008
    ..The engineered BNCs are a promising and powerful tool for efficient and cell-specific protein delivery...
  50. doi request reprint Biotinylated bionanocapsules for displaying diverse ligands toward cell-specific delivery
    Takuya Shishido
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada, Japan
    J Biochem 146:867-74. 2009
    ..Biotinylated BNC permit display of diverse ligands, and thus have potential as a versatile carrier for drug delivery to a variety of target cells...
  51. doi request reprint Role of N-terminal 28-amino-acid region of Rhizopus oryzae lipase in directing proteins to secretory pathway of Aspergillus oryzae
    Shinji Hama
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 79:1009-18. 2008
    ..These findings suggest that N28 facilitates protein translocation into ER and can be a promising candidate for improving heterologous protein production in A. oryzae...
  52. doi request reprint Ethanol production from cellulosic materials using cellulase-expressing yeast
    Shuhei Yanase
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Kobe, Japan
    Biotechnol J 5:449-55. 2010
    ..1 g/L for EG-, CBH-, and BGL-displaying yeast, which was higher than that of EG- and CBH-secreting yeast (1.6 g/L ethanol). Our results show that cell surface display is more suitable for direct ethanol fermentation from cellulose...
  53. pmc Improved production of homo-D-lactic acid via xylose fermentation by introduction of xylose assimilation genes and redirection of the phosphoketolase pathway to the pentose phosphate pathway in L-Lactate dehydrogenase gene-deficient Lactobacillus plantaru
    Kenji Okano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Appl Environ Microbiol 75:7858-61. 2009
    ..After 60 h of fermentation, 41.2 g/liter of d-lactic acid was produced from 50 g/liter of xylose...
  54. ncbi request reprint Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling
    Shota Togawa
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan
    J Biochem 147:867-73. 2010
    ..These results clearly show the importance of these asparagine residues in the agonist-specific signalling of hSSTR5, although it was not enough to identify the consequence of oligosaccharides...
  55. doi request reprint Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration
    Kengo Sasaki
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Bioresour Technol 147:84-8. 2013
    ..cerevisiae was double (5.34-6.44 g L(-1)) that compared with fermentation of liquid hydrolysate before membrane separation (2.75 g L(-1))...
  56. ncbi request reprint Improvement of cellulose-degrading ability of a yeast strain displaying Trichoderma reesei endoglucanase II by recombination of cellulose-binding domains
    Junji Ito
    Department of Chemical Science and Engineering, Faculty of Engineering, and Division of Molecular Science, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Biotechnol Prog 20:688-91. 2004
    ..This result indicates that the hydrolytic activity of yeast strains displaying recombinant EGII increases with increased binding ability to cellulose...
  57. pmc Display of alpha-amylase on the surface of Lactobacillus casei cells by use of the PgsA anchor protein, and production of lactic acid from starch
    Junya Narita
    Division of Molecular Science, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Appl Environ Microbiol 72:269-75. 2006
    ..These results indicate that efficient simultaneous saccharification and fermentation from soluble starch to lactic acid were carried out by recombinant L. casei cells with cell surface display of AmyA...
  58. doi request reprint A robust whole-cell biocatalyst that introduces a thermo- and solvent-tolerant lipase into Aspergillus oryzae cells: characterization and application to enzymatic biodiesel production
    Daisuke Adachi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Enzyme Microb Technol 52:331-5. 2013
    ....
  59. doi request reprint Site-specific protein labeling with amine-containing molecules using Lactobacillus plantarum sortase
    Takuya Matsumoto
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe, Japan
    Biotechnol J 7:642-8. 2012
    ..Our results demonstrate a promising method for expanding the capabilities of site-specific protein-small molecule modification...
  60. doi request reprint Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 108:128-33. 2012
    ..This study demonstrates that cellulases displayed on the yeast cell surface are capable of hydrolyzing cellulose that was not hydrolyzed by commercial cellulases, leading to increased sugar utilization for improved ethanol production...
  61. ncbi request reprint Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, Japan
    FEBS J 277:1982-95. 2010
    ....
  62. doi request reprint Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain
    Syun ichi Yamakawa
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 87:109-15. 2010
    ..26 U/ml during repeated fermentation. The highest ethanol productivity was 2.22 g/l/h at the fourth batch, and after ten cycles, ethanol productivity of more than 1.43 g/l/h was retained, as was alpha-amylase activity at 6.43 U/ml...
  63. doi request reprint Gene copy number and polyploidy on products formation in yeast
    Ryosuke Yamada
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 88:849-57. 2010
    ..In this mini-review, we focused on the effect of integrated gene copy number and the polyploidy on the productivity such as enzymatic activity and/or product yield...
  64. ncbi request reprint Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast
    Yusuke Iguchi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657 8501, Japan
    J Biochem 147:875-84. 2010
    ..This finding will be informative for constructing optimal yeast-based ligand screening systems to discriminate the cells on the basis of signalling levels...
  65. doi request reprint Glutamate production from β-glucan using endoglucanase-secreting Corynebacterium glutamicum
    Takeyuki Tsuchidate
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 90:895-901. 2011
    ..Using EG-secreting C. glutamicum, 178 mg/l of glutamate was produced from 15 g of β-glucan. This is the first report of glutamate fermentation from β-glucan using endoglucanase-secreting C. glutamicum...
  66. doi request reprint Creation of endoglucanase-secreting Streptomyces lividans for enzyme production using cellulose as the carbon source
    Shuhei Noda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:5711-20. 2013
    ..lividans transformant capable of coexpressing endoxylanase. The EG-secreting S. lividans transformants constructed here can be used to produce other useful compounds through cellulose fermentation...
  67. doi request reprint A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 135:513-22. 2013
    ..This article reviews recent advances in the development of microorganisms for the production of renewable chemicals and advanced biofuels, as well as ethanol, from lignocellulosic materials through consolidated bioprocessing...
  68. doi request reprint Sugar consumption and ethanol fermentation by transporter-overexpressed xylose-metabolizing Saccharomyces cerevisiae harboring a xyloseisomerase pathway
    Takanori Tanino
    Department of Chemical and Environmental Engineering, Graduate School of Engineering, Gunma University, Kiryu, Gunma, Japan
    J Biosci Bioeng 114:209-11. 2012
    ..cerevisiae harboring a xyloseisomerase-based pathway. Overexpression of transporter enhanced sugar consumption and ethanol production, and GXF1 was efficient for ethanol fermentation from both glucose and xylose...
  69. doi request reprint Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:2231-7. 2013
    ..5 g/L, one quarter of the cellulose remaining using the wild-type strain, a result of the alleviation of irreversible adsorption of cellulases on the crystalline cellulose...
  70. doi request reprint Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural
    Keisuke Fujitomi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 111:161-6. 2012
    ..Together, our findings demonstrate that PHO13 deletion is a simple, but effective, approach for improving cellulosic bioethanol production by S. cerevisiae...
  71. doi request reprint Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids
    Tomoya Sanda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 102:7917-24. 2011
    ..Long-term stability of ethanol production in the fermentation phase was not only attributed to the coexpression of TAL and FDH genes, but also the hybridization of haploid strains...
  72. pmc Cocktail delta-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains
    Ryosuke Yamada
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Microb Cell Fact 9:32. 2010
    ..To construct engineered yeast with efficient cellulose degradation, we developed a simple method to optimize cellulase expression levels, named cocktail delta-integration...
  73. doi request reprint Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction
    Tsutomu Tanaka
    Kobe University, Rokkodai cho, Nada, Japan
    J Biotechnol 145:79-83. 2010
    ..Our strategy provides a powerful tool for displaying functional molecules on yeast cell surfaces...
  74. doi request reprint The competitor-introduced Ggamma recruitment system, a new approach for screening affinity-enhanced proteins
    Nobuo Fukuda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan
    FEBS J 277:1704-12. 2010
    ..This new approach, called the competitor-introduced Ggamma recruitment system, will be useful for efficient discovery of rare valuable candidates hidden among excess ordinary ones...
  75. doi request reprint Direct production of cadaverine from soluble starch using Corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase
    Toshihiro Tateno
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 82:115-21. 2009
    ..Thus, we demonstrated that C. glutamicum has great potential to produce cadaverine from biomass resources...
  76. ncbi request reprint Construction of a Pichia pastoris cell-surface display system using Flo1p anchor system
    Takanori Tanino
    Division of Molecular Science and Material Engineering, Graduate School of Science and Technology, and Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, Nada Ku, Japan
    Biotechnol Prog 22:989-93. 2006
    ..Surprisingly, the activity of lipase displayed on the cells incubated at 60 degrees C was not only stable but also increased to about 6.5 times the initial value after 4 h incubation...
  77. ncbi request reprint Variation in biomass properties among rice diverse cultivars
    Fumio Matsuda
    Organization of Advanced Science and Technology, Kobe University, Kobe, Hyogo, Japan
    Biosci Biotechnol Biochem 75:1603-5. 2011
    ..The ethanol productivity from rice straws was evaluated employing a laboratory-scale method based on dilute acid-hydrolysis pretreatment. The results indicated significant variation in biomass properties among the cultivars...
  78. ncbi request reprint Deletion of MCD 4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae
    Jaruwan Maneesri
    Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, 3 3 138 Sugimoto, Sumiyoshi ku, Osaka 558 8585, Japan
    J Biosci Bioeng 99:354-60. 2005
    ..The result suggested that the mcd 4 deletion causes a decrease in GPI cell wall proteins levels. The mutation also caused a decrease in mannan levels and an increase in alkali-insoluble beta-1,6-glucan and chitin levels in the cell wall...
  79. doi request reprint Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst
    Hideyo Yoshida
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 91:1001-6. 2011
    ..6-fold) than either improvement individually for GSH enzymatic production using yeast. The improved whole-cell biocatalyst indicates its great potential for applications to other kinds of ATP-dependent bioproduction...
  80. doi request reprint Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells
    Takuya Shishido
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Bioorg Med Chem Lett 20:5726-31. 2010
    ..As a result, Z(HER2)-BNC-liposome complex demonstrated the delivery to HER2-expressing cells (SK-BR-3) with a high degree of specificity. This indicates that genetically engineered BNCs are promising carrier for cancer treatment...
  81. pmc Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Microb Cell Fact 10:2. 2011
    ..Thus, enhanced basic research is expected to identify target genes for improved weak acid tolerance...
  82. doi request reprint Ester synthesis reaction with CALB displaying yeast whole cell biocatalyst: effect of organic solvent and initial water content
    Takanori Tanino
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    J Biosci Bioeng 108:369-71. 2009
    ..Utilization of hydrophobic organic solvent improved the efficiency of the ester synthesis reaction. Also the initial water content was important for the expression of the ester synthesis activity of CALB displaying yeast whole cells...
  83. doi request reprint Protein-encapsulated bio-nanocapsules production with ER membrane localization sequences
    Yuya Nishimura
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    J Biotechnol 157:124-9. 2012
    ..This approach would be a useful method for encapsulating therapeutic candidate proteins into BNCs...
  84. doi request reprint Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains
    Hiroko Kato
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 94:1585-92. 2012
    ..8). Thus, the simple hybridization technique facilitated an increase in the xylose fermentation activity...
  85. pmc Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase
    Hisayori Shigechi
    Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 6547 8501, Japan
    Appl Environ Microbiol 70:5037-40. 2004
    ..In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch...
  86. ncbi request reprint Effect of flocculation on performance of arming yeast in direct ethanol fermentation
    Khaw Teik Seong
    Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka, 565 0871, Japan
    Appl Microbiol Biotechnol 73:60-6. 2006
    ....
  87. doi request reprint Homo-D-lactic acid production from mixed sugars using xylose-assimilating operon-integrated Lactobacillus plantarum
    Shogo Yoshida
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Japan
    Appl Microbiol Biotechnol 92:67-76. 2011
    ..This is the first report that describes homo-D-lactic acid fermentation from mixed sugars without carbon catabolite repression using the xylose-assimilating pathway integrated into lactic acid bacteria...
  88. doi request reprint Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production
    Daisuke Adachi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Bioresour Technol 102:6723-9. 2011
    ..Moreover, an ME content of more than 90% was maintained during 10 repeated batch cycles. The whole-cell biocatalyst developed in this work would be promising biocatalysts for efficient biodiesel production...
  89. doi request reprint Construction of a novel detection system for protein-protein interactions using yeast G-protein signaling
    Nobuo Fukuda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan
    FEBS J 276:2636-44. 2009
    ..In conclusion, our system appears to be a reliable and versatile technique for detection of protein-protein interactions, and may prove useful in future protein interaction studies...
  90. doi request reprint Improvement of a Candida antarctica lipase B-displaying yeast whole-cell biocatalyst and its application to the polyester synthesis reaction
    Takanori Tanino
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 82:59-66. 2009
    ..From these results, it appears that the use of CALB-displaying yeast cells could be useful for the polyester synthesis reaction, with reduced by-product production...
  91. pmc Creation of a cellooligosaccharide-assimilating Escherichia coli strain by displaying active beta-glucosidase on the cell surface via a novel anchor protein
    Tsutomu Tanaka
    Organization of Advanced Science and Technology, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Appl Environ Microbiol 77:6265-70. 2011
    ..coli cell surface using a novel anchor protein named Blc. This strain was grown successfully on 0.2% cellobiose, and the optical density at 600 nm (OD(600)) was 1.05 after 20 h...
  92. ncbi request reprint Lipase localization in Rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production
    Shinji Hama
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    J Biosci Bioeng 101:328-33. 2006
    ..oryzae cells...
  93. ncbi request reprint Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch
    Teik Seong Khaw
    Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka, 565 0871, Japan
    Appl Microbiol Biotechnol 70:573-9. 2006
    ..In all systems, the rate-limiting step was the conversion of starch to oligosaccharide because oligosaccharide and glucose were not accumulated throughout the fermentations...
  94. ncbi request reprint Improvement of protein production in lactic acid bacteria using 5'-untranslated leader sequence of slpA from Lactobacillus acidophilus. Improvement in protein production using UTLS
    Junya Narita
    Division of Molecular Science, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 73:366-73. 2006
    ..The current study showed that the improvement in protein production using the UTLS could be applied to the expression system in the lactic acid bacteria...
  95. ncbi request reprint Enhancement of activity of lipase-displaying yeast cells and their application to optical resolution of (R,S)-1-benzyloxy-3-chloro-2-propyl monosuccinate
    Yurie Nakamura
    Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, Nada Ku, Japan
    Biotechnol Prog 22:998-1002. 2006
    ..These results demonstrate that ROL-displaying yeast cells with enhanced activity by incubation in distilled water are very effective in industrial bioconversion processes...
  96. ncbi request reprint Enhancement of ethanol production by promoting surface contact between starch granules and arming yeast in direct ethanol fermentation
    Teik Seong Khaw
    Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biosci Bioeng 103:95-7. 2007
    ..These results indicate that the surface contact between starch granules and yeast cells is important for increasing the apparent specific activity of alpha-amylase, which was the rate-limiting factor of the direct fermentation...
  97. doi request reprint Over-production of various secretory-form proteins in Streptomyces lividans
    Shuhei Noda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Protein Expr Purif 73:198-202. 2010
    ..Three independent proteins were thus successfully expressed and purified, and we expect to use this system for the expression of other valuable heterologous proteins...
  98. pmc System using tandem repeats of the cA peptidoglycan-binding domain from Lactococcus lactis for display of both N- and C-terminal fusions on cell surfaces of lactic acid bacteria
    Kenji Okano
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Appl Environ Microbiol 74:1117-23. 2008
    ..In addition, cA-AmyA fusion proteins were successfully displayed on the cell surfaces of Lactobacillus plantarum and Lactobacillus casei...
  99. doi request reprint Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives
    Seiji Shibasaki
    Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, Minatojima, Chuo Ku, Kobe, 650 8530, Japan
    Appl Microbiol Biotechnol 80:735-43. 2008
    ..These demonstrations suggest that the yeast PCA system based on DHFR can be one of good, convenient, and inexpensive tools for investigating eukaryotic protein-protein interactions in vivo...
  100. ncbi request reprint Production of L-Lysine from starch by Corynebacterium glutamicum displaying alpha-amylase on its cell surface
    Toshihiro Tateno
    Department of Molecular Science and Material Engineering, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 74:1213-20. 2007
    ..The results shown in the current study demonstrate that C. glutamicum displaying alpha-amylase has a potential to directly convert soluble starch to amino acids...