Norihiro Kobayashi

Summary

Affiliation: Kobe Pharmaceutical University
Country: Japan

Publications

  1. doi Anti-estradiol-17beta single-chain Fv fragments: Generation, characterization, gene randomization, and optimized phage display
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1 Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Steroids 73:1485-99. 2008
  2. doi Toward in vivo imaging of heart disease using a radiolabeled single-chain Fv fragment targeting tenascin-C
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Anal Chem 83:9123-30. 2011
  3. ncbi [Antibody engineering for advanced molecular recognition]
    Norihiro Kobayashi
    Kobe Pharmaceutical University, Higashinada ku, Kobe City, Japan
    Yakugaku Zasshi 127:41-2. 2007
  4. doi "Cleavable" hapten-biotin conjugates: preparation and use for the generation of anti-steroid single-domain antibody fragments
    Norihiro Kobayashi
    Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Anal Biochem 387:257-66. 2009
  5. ncbi [Antibody engineering-based approach for hapten immunometric assays with high sensitivity]
    Norihiro Kobayashi
    Kobe Pharmaceutical University, Higashinada ku, Kobe City, Japan
    Yakugaku Zasshi 127:55-69. 2007
  6. doi Two-step in vitro antibody affinity maturation enables estradiol-17beta assays with more than 10-fold higher sensitivity
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Anal Chem 82:1027-38. 2010
  7. doi Oligosaccharide-assisted direct immunosensing of small molecules
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Anal Chem 82:4333-6. 2010
  8. doi Antibody engineering toward high-sensitivity high-throughput immunosensing of small molecules
    Norihiro Kobayashi
    Kobe Pharmaceutical University, Motoyama Kitamachi, Higashinada ku, Japan
    Analyst 136:642-51. 2011
  9. ncbi Generation of a single-chain Fv fragment for the monitoring of deoxycholic acid residues anchored on endogenous proteins
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Steroids 70:285-94. 2005
  10. ncbi Immunoenzymometric assay for a small molecule,11-deoxycortisol, with attomole-range sensitivity employing an scFv-enzyme fusion protein and anti-idiotype antibodies
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Kobe, 658 8558, Japan
    Anal Chem 78:2244-53. 2006

Collaborators

Detail Information

Publications34

  1. doi Anti-estradiol-17beta single-chain Fv fragments: Generation, characterization, gene randomization, and optimized phage display
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1 Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Steroids 73:1485-99. 2008
    ..A soluble scFv clone (scFv#m1-e7) with a mutated amino acid (I-->V) in CDR L1, isolated from this library, showed threefold higher affinity (K(a)=2.6 x 10(8)M(-1)) than that of scFv#4-4...
  2. doi Toward in vivo imaging of heart disease using a radiolabeled single-chain Fv fragment targeting tenascin-C
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Anal Chem 83:9123-30. 2011
    ..Single photon emission computed tomography (SPECT) provided in vivo cardiac images that coincided with the ex vivo observations. Our results will promote advances in diagnostic strategies for heart disease...
  3. ncbi [Antibody engineering for advanced molecular recognition]
    Norihiro Kobayashi
    Kobe Pharmaceutical University, Higashinada ku, Kobe City, Japan
    Yakugaku Zasshi 127:41-2. 2007
  4. doi "Cleavable" hapten-biotin conjugates: preparation and use for the generation of anti-steroid single-domain antibody fragments
    Norihiro Kobayashi
    Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Anal Biochem 387:257-66. 2009
    ..Thorough washing off of nonspecific phages and subsequent DTT treatment provided a phagemid clone that displayed a mutated sdAb with improved binding properties...
  5. ncbi [Antibody engineering-based approach for hapten immunometric assays with high sensitivity]
    Norihiro Kobayashi
    Kobe Pharmaceutical University, Higashinada ku, Kobe City, Japan
    Yakugaku Zasshi 127:55-69. 2007
    ..This review surveys the background and recent approach for subfemtomole-range determination of haptens using novel immunometric assay methods. Our studies for the development of hapten immunometric assays are also described...
  6. doi Two-step in vitro antibody affinity maturation enables estradiol-17beta assays with more than 10-fold higher sensitivity
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Anal Chem 82:1027-38. 2010
    ..47 pg (1.7 fmol)/assay] in a competitive enzyme-linked immunosorbent assay. Cross-reactivity with selected E(2)-related endogenous steroids strongly suggested that scFv#m2-c4 has improved specificity compared to conventional antibodies...
  7. doi Oligosaccharide-assisted direct immunosensing of small molecules
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Anal Chem 82:4333-6. 2010
    ..These scFvs enabled novel "semisandwich-type" immunometric assays of haptens with nanomole-range sensitivities...
  8. doi Antibody engineering toward high-sensitivity high-throughput immunosensing of small molecules
    Norihiro Kobayashi
    Kobe Pharmaceutical University, Motoyama Kitamachi, Higashinada ku, Japan
    Analyst 136:642-51. 2011
    ..In this review, we summarize recent successful developments and applications of engineered antibodies for sensitive and high-throughput hapten sensing...
  9. ncbi Generation of a single-chain Fv fragment for the monitoring of deoxycholic acid residues anchored on endogenous proteins
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Steroids 70:285-94. 2005
    ....
  10. ncbi Immunoenzymometric assay for a small molecule,11-deoxycortisol, with attomole-range sensitivity employing an scFv-enzyme fusion protein and anti-idiotype antibodies
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1, Motoyama Kitamachi, Kobe, 658 8558, Japan
    Anal Chem 78:2244-53. 2006
    ..This IEMA afforded an extremely low detection limit (20 amol/assay), a very wide measurable range, and practical specificity. The plasma 11-DC levels determined for healthy subjects were validated as reliable...
  11. doi Correlation between reduction potentials and inhibitions of Epstein-Barr virus activation by anthraquinone derivatives
    Junko Koyama
    Faculty of Pharmaceutical Sciences, Kobe Pharmaceutical University, Higashinada, Kobe 658 8558, Japan
    Bioorg Med Chem Lett 18:4106-9. 2008
    ..It was further shown that the correlation could be enhanced by including LUMO energy and the number of hydroxy groups as additional parameters...
  12. doi Development of an LC-ESI-MS/MS method for the determination of histamine: application to the quantitative measurement of histamine degranulation by KU812 cells
    Junko Koyama
    Kobe Pharmaceutical University, Higashinada, Kobe 658 8558, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 877:207-12. 2009
    ..Of these compounds, taxiresinol and isotaxiresinol were more inhibitory than sodium cromoglicate...
  13. ncbi Dissociation-independent selection of high-affinity anti-hapten phage antibodies using cleavable biotin-conjugated haptens
    Norihiro Kobayashi
    Kobe Pharmaceutical University, 4 19 1 Motoyama Kitamachi, Higashinada ku, Kobe 658 8558, Japan
    Anal Biochem 347:287-96. 2005
    ..This method will be applicable for selection of extra-high-affinity phage antibodies against a wide variety of haptens...
  14. doi Characterization of emodin metabolites in Raji cells by LC-APCI-MS/MS
    Junko Koyama
    Kobe Pharmaceutical University, Higashinada, Kobe 658 8558, Japan
    Bioorg Med Chem 17:7493-9. 2009
    ..In addition to the major metabolite 8-O-methylemodin, four other metabolites were assigned as omega-hydroxyemodin, 3-O-methyl-omega-hydroxyemodin, 3-O-methylemodin (physcion), and chrysophanol...
  15. ncbi Simultaneous determination of anthraquinones in rhubarb by high-performance liquid chromatography and capillary electrophoresis
    Junko Koyama
    Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Chromatogr A 1145:183-9. 2007
    ....
  16. ncbi Correlation between reduction potentials and inhibitory effects on Epstein-Barr virus activation by emodin derivatives
    Junko Koyama
    Faculty of Pharmaceutical Sciences, Kobe Pharmaceutical University, Higashinada, Kobe 658 8558, Japan
    Cancer Lett 241:263-7. 2006
    ..The electronic properties, i.e. LUMO energy and atomic charges of carbon at the 9-position (C(9)) and oxygen at the 11-position (O(11)), may also be useful for estimating the inhibitory effect on EBV-EA activation...
  17. ncbi Correlation between reduction potentials and inhibitory effects on Epstein-Barr virus activation of poly-substituted anthraquinones
    Junko Koyama
    Faculty of Pharmaceutical Sciences, Kobe Pharmaceutical University, Higashinada, Kobe 658 8558, Japan
    Cancer Lett 225:193-8. 2005
    ..It has been further shown that the correlation can be enhanced by introducing log P as an additional parameter...
  18. ncbi Simultaneous determination of anthraquinones, their 8-beta-D-glucosides, and sennosides of Rhei Rhizoma by capillary electrophoresis
    Junko Koyama
    Kobe Pharmaceutical University, Kobe 658 8558, Japan
    Chem Pharm Bull (Tokyo) 53:573-5. 2005
    ..The running electrolyte used in this method was 0.005 M alpha-cyclodextrin in 0.03 M borate buffer (pH 10.0) containing 20% acetonitrile, with an applied voltage of 20 kV...
  19. ncbi Structure-activity relations of azafluorenone and azaanthraquinone as antimicrobial compounds
    Junko Koyama
    Faculty of Pharmaceutical Sciences, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Bioorg Med Chem Lett 15:1079-82. 2005
    ..In addition, correlations among reduction potential, hydrophobic parameter, and antimicrobial activity were discussed...
  20. ncbi Correlation of redox potentials and inhibitory effects on Epstein-Barr virus activation of 2-azaanthraquinones
    Junko Koyama
    Faculty of Pharmaceutical Sciences, Kobe Pharmaceutical University, Higashinada, Kobe 658 8558, Japan
    Cancer Lett 212:1-6. 2004
    ..It has been further shown that the correlation can be enhanced by introducing an electronic properties, i.e. the atomic charges at the C5 and O12 atoms in the quinone skeleton ring and the HOMO energy as additional parameters...
  21. ncbi Correlation of redox potentials and inhibitory effects on Epstein-Barr virus activation of naphthoquinones
    Junko Koyama
    Faculty of Pharmaceutical Sciences, Kobe Pharmaceutical University, Higashinada, Kobe 658, Japan
    Cancer Lett 201:25-30. 2003
    ..It has been further shown that the correlation can be enhanced by introducing an electronic property, i.e. the atomic charges at the C(4) and O(10) atoms in the quinone skeleton ring as additional parameters...
  22. ncbi Capillary electrophoresis for simultaneous determination of emodin, chrysophanol, and their 8-beta-D-glucosides
    Junko Koyama
    Kobe Pharmaceutical University, Higashinada ku, Japan
    Chem Pharm Bull (Tokyo) 51:418-20. 2003
    ..The running electrolyte used in this method was 0.005 M alpha-cyclodextrin in 0.03 M borate buffer (pH 10.5) containing 10% acetonitrile, with an applied voltage of 20 kV...
  23. doi "Breeding" diagnostic antibodies for higher assay performance: a 250-fold affinity-matured antibody mutant targeting a small biomarker
    Hiroyuki Oyama
    Kobe Pharmaceutical University, Higashinada ku, Kobe, Japan
    Anal Chem 85:4930-7. 2013
    ..The average human serum E2 levels, determined after acetonitrile extraction, were compatible with reported reference ranges. The present results will prompt a new era for preparing diagnostic reagents...
  24. doi Correlation between oxidation potentials and inhibitory effects on Epstein-Barr virus activation of flavonoids
    Junko Koyama
    Faculty of Pharmaceutical Sciences, Kobe Pharmaceutical University, Higashinada, Kobe 658 8558, Japan
    Cancer Lett 263:61-6. 2008
    ..Furthermore, multiple regression analysis revealed that the solvent-accessible surface area (SASA) was a useful parameter for estimating the inhibitory effects of flavonoids on EBV-EA activation...
  25. ncbi [Hapten immunoassays: current status and future prospect]
    Norihiro Kobayashi
    Department of Bioanalytical Chemistry, Kobe Pharmaceutical University, Kobe, Japan
    Rinsho Byori 56:525-35. 2008
    ..This review summarizes the current status and future prospect of "hapten immunoassays"...
  26. ncbi Antiallergic activity of aqueous extracts and constituents of Taxus yunnanensis
    Junko Koyama
    Kobe Pharmaceutical University, Japan
    Biol Pharm Bull 29:2310-2. 2006
    ..A new neolignan, 2-[2-hydroxy-5-(3-hydroxypropyl)-3-methoxyphenyl]-1-(4-hydroxy-3-methoxyphenyl)propane-1,3-diol, was isolated from the wood of Taxus yunnanensis...
  27. ncbi Analysis of the antigen binding site of anti-deoxycholate monoclonal antibody using a novel affinity labeling reagent, acyl adenylate
    Nariyasu Mano
    Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba ku, Sendai 980 8578, Japan
    Biochemistry 43:2041-8. 2004
    ..In addition, LC/ESI-MS/MS analysis of the labeled peptide obtained by enzymatic digestion and affinity extraction allowed the identification of the structure surrounding the antigen binding site...
  28. ncbi Monoclonal anti-idiotype antibodies recognizing the variable region of a high-affinity antibody against 11-deoxycortisol. Production, characterization and application to a sensitive noncompetitive immunoassay
    Norihiro Kobayashi
    Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba ku, 980 8578, Sendai, Japan
    J Immunol Methods 274:63-75. 2003
    ..Appropriate serum 11-DC levels were obtained for normal subjects [0.16+/-0.09 (S.D.) microg/l (n=6), ranging from 0.086 to 0.316 microg/l] using the present assay system...
  29. ncbi [Noncompetitive immunoenzymometric assays for a hapten molecule employing anti-idiotype antibodies]
    Toshifumni Niwa
    Department of Medical Technology, School of Health Sciences Tohoku University, Sendai, Japan
    Rinsho Byori 56:536-43. 2008
    ..The detection limit of this novel immunometric assay was approximately 500-times lower than a competitive radioimmunoassay based on the corresponding anti-11-DC antibody...
  30. ncbi A nonenzymatic modification of the amino-terminal domain of histone H3 by bile acid acyl adenylate
    Nariyasu Mano
    Graduate School of Pharmaceutical Sciences, Tohoku University, 101 Seiryo machi, Aobayama, Aoba ku, Sendai 980 8578, Japan
    J Biol Chem 279:55034-41. 2004
    ..This novel modification, formation of a bile acid adduct on the histone H3 amino-terminal tail domain through an active acyl adenylate, may relate to the carcinogenesis-promoting effects of secondary bile acids...
  31. ncbi Enzyme-linked immunosorbent assay for monitoring toxic dioxin congeners in milk based on a newly generated monoclonal anti-dioxin antibody
    Mitsunobu Okuyama
    Food and Drug Safety Center, Hatano Research Institute, 729 5, Ochiai, Hadano, Kanagawa 257 8523, Japan
    Anal Chem 76:1948-56. 2004
    ....
  32. ncbi Generation of a novel monoclonal antibody against cortisol-[C-4]-bovine serum albumin conjugate: application to enzyme-linked immunosorbent assay for urinary and serum cortisol
    Norihiro Kobayashi
    Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba ku, Sendai 980 8578, Japan
    Anal Sci 18:1309-14. 2002
    ..0 +/- 17.0 microg/day (n = 7) and 80.8 +/- 38.9 ng/mL (n = 10), respectively, both of which are in the reference range...
  33. ncbi A monoclonal antibody-based enzyme-linked immunosorbent assay of glycolithocholic acid sulfate in human urine for liver function test
    Norihiro Kobayashi
    Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba ku, 980 8578, Sendai, Japan
    Steroids 67:827-33. 2002
    ..The present 'monoclonal ELISA' is predicted to be useful as a novel noninvasive diagnostic tool for liver function and hepatobiliary diseases...
  34. ncbi Single-chain Fv fragments derived from an anti-11-deoxycortisol antibody. Affinity, specificity, and idiotype analysis
    Norihiro Kobayashi
    Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Sendai, Japan
    Steroids 67:733-42. 2002
    ..These results suggest that the present scFvs retain the three-dimensional structure of the paratope of the original monoclonal antibody...