Hiroshi Kitagawa

Summary

Affiliation: Kobe Pharmaceutical University
Country: Japan

Publications

  1. pmc Human glycosaminoglycan glucuronyltransferase I gene and a related processed pseudogene: genomic structure, chromosomal mapping and characterization
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 358:539-46. 2001
  2. doi request reprint Sulfation of the galactose residues in the glycosaminoglycan-protein linkage region by recombinant human chondroitin 6-O-sulfotransferase-1
    Hiroshi Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 283:27438-43. 2008
  3. ncbi request reprint Expression of rib-1, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes, is indispensable for heparan sulfate synthesis and embryonic morphogenesis
    Hiroshi Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 282:8533-44. 2007
  4. ncbi request reprint Heparan sulfate polymerization in Drosophila
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 281:1929-34. 2006
  5. ncbi request reprint Nematode chondroitin polymerizing factor showing cell-/organ-specific expression is indispensable for chondroitin synthesis and embryonic cell division
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 279:53755-61. 2004
  6. ncbi request reprint Identification and characterization of three Drosophila melanogaster glucuronyltransferases responsible for the synthesis of the conserved glycosaminoglycan-protein linkage region of proteoglycans. Two novel homologs exhibit broad specificity toward oligo
    Byung Taek Kim
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:9116-24. 2003
  7. doi request reprint Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: chondroitin polymerization is achieved by multiple enzyme complexes consisting of chondroitin synthase family members
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 283:11396-406. 2008
  8. doi request reprint FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region
    Toshiyasu Koike
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 421:157-62. 2009
  9. ncbi request reprint Molecular cloning and expression of a second chondroitin N-acetylgalactosaminyltransferase involved in the initiation and elongation of chondroitin/dermatan sulfate
    Toru Uyama
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:3072-8. 2003
  10. pmc EXTL2, a member of the EXT family of tumor suppressors, controls glycosaminoglycan biosynthesis in a xylose kinase-dependent manner
    Satomi Nadanaka
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 288:9321-33. 2013

Collaborators

Detail Information

Publications68

  1. pmc Human glycosaminoglycan glucuronyltransferase I gene and a related processed pseudogene: genomic structure, chromosomal mapping and characterization
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 358:539-46. 2001
    ..The pseudogene was localized to chromosome 3. The human genome therefore contains two related GlcAT-I genes that are located on separate chromosomes...
  2. doi request reprint Sulfation of the galactose residues in the glycosaminoglycan-protein linkage region by recombinant human chondroitin 6-O-sulfotransferase-1
    Hiroshi Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 283:27438-43. 2008
    ..This is the first identification of the sulfotransferase responsible for the sulfation of galactose residues in the glycosaminoglycan-protein linkage region...
  3. ncbi request reprint Expression of rib-1, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes, is indispensable for heparan sulfate synthesis and embryonic morphogenesis
    Hiroshi Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 282:8533-44. 2007
    ..These findings also show that heparan sulfate is essential for post-gastrulation morphogenic movement of embryonic cells and is indispensable for ensuring the normal spatial organization of differentiated tissues and organs...
  4. ncbi request reprint Heparan sulfate polymerization in Drosophila
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 281:1929-34. 2006
    ..Thus, all three EXT members in Drosophila, TTV, SOTV, and BOTV, are required for the biosynthesis of full-length HS in Drosophila...
  5. ncbi request reprint Nematode chondroitin polymerizing factor showing cell-/organ-specific expression is indispensable for chondroitin synthesis and embryonic cell division
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 279:53755-61. 2004
    ..These findings are consistent with the notion that chondroitin is involved in the organogenesis of the vulva and maturation of the gonad and also indicative of an involvement in distal tip cell migration and neural development...
  6. ncbi request reprint Identification and characterization of three Drosophila melanogaster glucuronyltransferases responsible for the synthesis of the conserved glycosaminoglycan-protein linkage region of proteoglycans. Two novel homologs exhibit broad specificity toward oligo
    Byung Taek Kim
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:9116-24. 2003
    ....
  7. doi request reprint Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: chondroitin polymerization is achieved by multiple enzyme complexes consisting of chondroitin synthase family members
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 283:11396-406. 2008
    ..Based on these results, we renamed CSGlcA-T chondroitin synthase-3 (ChSy-3)...
  8. doi request reprint FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region
    Toshiyasu Koike
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 421:157-62. 2009
    ..These results suggest that FAM20B regulates the number of glycosaminoglycan chains by phosphorylating the xylose residue in the glycosaminoglycan-protein linkage region of proteoglycans...
  9. ncbi request reprint Molecular cloning and expression of a second chondroitin N-acetylgalactosaminyltransferase involved in the initiation and elongation of chondroitin/dermatan sulfate
    Toru Uyama
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:3072-8. 2003
    ....
  10. pmc EXTL2, a member of the EXT family of tumor suppressors, controls glycosaminoglycan biosynthesis in a xylose kinase-dependent manner
    Satomi Nadanaka
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 288:9321-33. 2013
    ..These results indicate that EXTL2 functions to suppress GAG biosynthesis that is enhanced by a xylose kinase and that the EXTL2-dependent mechanism that regulates GAG biosynthesis might be a "quality control system" for proteoglycans...
  11. ncbi request reprint Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization
    Hiroshi Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:23666-71. 2003
    ..Thus, the ChPF is required for chondroitin polymerizing activity of mammalian ChSy...
  12. ncbi request reprint [Biosynthesis of the sulfated glycosaminoglycans in relation to human diseases]
    Hiroshi Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    Seikagaku 76:1175-90. 2004
  13. ncbi request reprint In vitro heparan sulfate polymerization: crucial roles of core protein moieties of primer substrates in addition to the EXT1-EXT2 interaction
    Byung Taek Kim
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyasmakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:41618-23. 2003
    ....
  14. ncbi request reprint Functions of chondroitin sulfate/dermatan sulfate chains in brain development. Critical roles of E and iE disaccharide units recognized by a single chain antibody GD3G7
    Anurag Purushothaman
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 282:19442-52. 2007
    ..Four discrete decasaccharide epitopic sequences were identified. The antibody GD3G7 has broad applications in investigations of CS/DS chains during the central nervous system's development and under various pathological conditions...
  15. pmc Impairment of embryonic cell division and glycosaminoglycan biosynthesis in glucuronyltransferase-I-deficient mice
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 285:12190-6. 2010
    ..Taken together, these results suggest that chondroitin sulfate in mammals, as with non-sulfated chondroitin in C. elegans, is indispensable for embryonic cell division...
  16. ncbi request reprint Specificities of three distinct human chondroitin/dermatan N-acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor: implication of differential roles in dermatan sulfate biosynthesis
    Tadahisa Mikami
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 278:36115-27. 2003
    ..The isolated oligosaccharide structures also suggest that 4-O-sulfation promotes subsequent 4-O-sulfation of GalNAc in the neighboring disaccharide unit...
  17. doi request reprint Chondroitin 4-O-sulfotransferase-1 regulates the chain length of chondroitin sulfate in co-operation with chondroitin N-acetylgalactosaminyltransferase-2
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, Kobe 658 8558, Japan
    Biochem J 434:321-31. 2011
    ..Overall, these results suggest that the chain length of CS is regulated by C4ST-1 and ChGn-2 and that the enzymatic activities of these proteins play a critical role in CS elongation...
  18. pmc 2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis
    Yuko Tone
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 283:16801-7. 2008
    ..The results indicate the possible involvement of these modifications in the processing and maturation of the growing linkage region oligosaccharide required for the assembly of GAG chains...
  19. ncbi request reprint Recent advances in the structural biology of chondroitin sulfate and dermatan sulfate
    Kazuyuki Sugahara
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, 658 8558, Kobe, Japan
    Curr Opin Struct Biol 13:612-20. 2003
    ....
  20. doi request reprint Chondroitin 4-O-sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish
    Shuji Mizumoto
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 419:387-99. 2009
    ..These results suggest that 4-O-sulfated CS formed by C4ST-1 is essential for somitic muscle differentiation and motor axon guidance in zebrafish development...
  21. ncbi request reprint [Biosynthetic mechanism of the bioactive sulfated glycosaminoglycans]
    Hiroshi Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    Yakugaku Zasshi 122:435-50. 2002
    ....
  22. doi request reprint Chondroitin 4-O-sulfotransferase-1 modulates Wnt-3a signaling through control of E disaccharide expression of chondroitin sulfate
    Satomi Nadanaka
    Department of Biochemistry, Kobe Pharmaceutical University, Kobe 658 8558, Japan
    J Biol Chem 283:27333-43. 2008
    ..These results suggest that CS-E-like structures synthesized by C4ST-1 participate in Wnt-3a signaling and modulate the physiological events caused by Wnt-3a signals...
  23. pmc Down-regulation of chondroitin 4-O-sulfotransferase-1 by Wnt signaling triggers diffusion of Wnt-3a
    Satomi Nadanaka
    Department of Biochemistry, Kobe Pharmaceutical University, Kobe 658 8558, Japan
    J Biol Chem 286:4199-208. 2011
    ..These results demonstrated that C4ST-1 is a key downstream target of Wnt signaling that regulates Wnt diffusion from Wnt-producing cells...
  24. pmc Chondroitin sulfate is a crucial determinant for skeletal muscle development/regeneration and improvement of muscular dystrophies
    Tadahisa Mikami
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 287:38531-42. 2012
    ..Our data suggest that the control of CS abundance is a promising new therapeutic approach for the treatment of skeletal muscle injury and progressive muscular dystrophies...
  25. ncbi request reprint Chondroitin 4-O-sulfotransferase-1 regulates E disaccharide expression of chondroitin sulfate required for herpes simplex virus infectivity
    Toru Uyama
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 281:38668-74. 2006
    ..These results suggest that C4ST-1 regulates the expression of the E disaccharide unit and the length of CS chains, the features that facilitate infection of cells by HSV-1...
  26. pmc Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 403:545-52. 2007
    ..Altogether these results suggest that chondroitin polymerization is achieved by multiple combinations of ChSy-1, CSS3 and ChPF. Based on these characteristics, we have renamed CSS3 ChSy-2 (chondroitin synthase-2)...
  27. doi request reprint Biosynthesis of heparan sulfate in EXT1-deficient cells
    Megumi Okada
    Department of Biochemistry, Kobe Pharmaceutical University, Kobe 658 8558, Japan
    Biochem J 428:463-71. 2010
    ..These findings indicate that the transfer of the first GlcNAc residue to the linkage region by EXTL2 is critically required for the biosynthesis of HS in cells deficient in EXT1...
  28. doi request reprint Roles of EXTL2, a member of the EXT family of tumour suppressors, in liver injury and regeneration processes
    Satomi Nadanaka
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 454:133-45. 2013
    ..Taken together, the results of the present study indicated that EXTL2-mediated regulation of GAG synthesis was important to the tissue regeneration processes that follow liver injury. ..
  29. ncbi request reprint Demonstration of a novel gene DEXT3 of Drosophila melanogaster as the essential N-acetylglucosamine transferase in the heparan sulfate biosynthesis: chain initiation and elongation
    Byung Taek Kim
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 277:13659-65. 2002
    ..These results suggest the essential roles of DEXT3, its human ortholog EXTL3, and the C. elegans ortholog rib-2 in the biosynthesis of heparan sulfate and heparin, if present, in the respective organisms...
  30. doi request reprint Persistent cortical plasticity by upregulation of chondroitin 6-sulfation
    Shinji Miyata
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe, Japan
    Nat Neurosci 15:414-22, S1-2. 2012
    ..Our results indicate that the critical period for cortical plasticity is regulated by the 4S/6S ratio of CSPGs, which determines the maturation of parvalbumin-expressing interneurons...
  31. doi request reprint Chondroitin sulfate-E fine-tunes osteoblast differentiation via ERK1/2, Smad3 and Smad1/5/8 signaling by binding to N-cadherin and cadherin-11
    Toshiyasu Koike
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem Biophys Res Commun 420:523-9. 2012
    ..Collectively, these results suggest that CS-E is a selective ligand for the potential CS receptors, N-cadherin and cadherin-11, leading to osteoblast differentiation of MC3T3-E1 cells...
  32. doi request reprint Chondroitin 4-O-sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    Biochem J 441:697-705. 2012
    ..Taken together, the results of the present study indicate that C4ST-2 plays a key role in regulating levels of CS synthesized via ChGn-1...
  33. doi request reprint Mice deficient in glucuronyltransferase-I
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, Kobe, Japan
    Prog Mol Biol Transl Sci 93:19-34. 2010
    ..Here, we summarize the recent knowledge on the roles of GlcAT-I in mammalian GAG biosynthesis and embryonic cell division using GlcAT-I knock-out mice...
  34. doi request reprint A chondroitin synthase-1 (ChSy-1) missense mutation in a patient with neuropathy impairs the elongation of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1
    Tomomi Izumikawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe, Japan
    Biochim Biophys Acta 1830:4806-12. 2013
    ..Chondroitin synthase-1 has two glycosyltransferase activities: it acts as a GlcUA and a GalNAc transferase and is responsible for adding repeated disaccharide units to growing chondroitin sulfate chains...
  35. doi request reprint Heparan sulphate biosynthesis and disease
    Satomi Nadanaka
    Department of Biochemistry, Kobe Pharmaceutical University, Kobe 658 8558, Japan
    J Biochem 144:7-14. 2008
    ..This review suggests that HS biosynthetic enzymes would be potential candidates for drug targets in various diseases...
  36. doi request reprint Contactin-1 is a functional receptor for neuroregulatory chondroitin sulfate-E
    Tadahisa Mikami
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558 and CREST, The Japan Science and Technology Agency, Kawaguchi 332 0012, Japan
    J Biol Chem 284:4494-9. 2009
    ..Our data provide the first evidence that biological functions of CS are exerted through the CS receptor-mediated signaling pathway(s)...
  37. ncbi request reprint Molecular cloning and expression of glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658, Japan
    J Biol Chem 273:6615-8. 1998
    ....
  38. ncbi request reprint Recent advances in the study of the biosynthesis and functions of sulfated glycosaminoglycans
    K Sugahara
    Kobe Pharmaceutical University, Department of Biochemistry, Higashinada ku, 658 8558, Kobe, Japan
    Curr Opin Struct Biol 10:518-27. 2000
    ..These biosynthetic mechanisms are crucial to the expression and regulation of the biological functions of glycosaminoglycans in development and pathophysiology...
  39. ncbi request reprint Heparin and heparan sulfate biosynthesis
    Kazuyuki Sugahara
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    IUBMB Life 54:163-75. 2002
    ....
  40. ncbi request reprint Characterization of recombinant human glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans
    Y Tone
    Department of Biochemistry, Kobe Pharmaceutical University, 4 19 1 Motoyamakita machi, Higashinada ku, Kobe, Japan
    FEBS Lett 459:415-20. 1999
    ..These results suggested that the high expression of the GlcAT-I gene rendered the cells capable of synthesizing the HNK-1 epitope...
  41. doi request reprint Biosynthesis and function of chondroitin sulfate
    Tadahisa Mikami
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe, Japan
    Biochim Biophys Acta 1830:4719-33. 2013
    ..Comprehensive understanding of the regulatory mechanisms for CS biosynthesis and its catabolic processes is required in order to understand those functions...
  42. ncbi request reprint Molecular cloning and expression of human chondroitin N-acetylgalactosaminyltransferase: the key enzyme for chain initiation and elongation of chondroitin/dermatan sulfate on the protein linkage region tetrasaccharide shared by heparin/heparan sulfate
    Toru Uyama
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 277:8841-6. 2002
    ..Thus, more than two distinct enzymes forming the novel gene family are required for chain initiation and elongation in chondroitin/dermatan sulfate as in the biosynthesis of heparin/heparan sulfate...
  43. ncbi request reprint Structural variations in the glycosaminoglycan-protein linkage region of recombinant decorin expressed in Chinese hamster ovary cells
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Japan
    Glycobiology 7:1175-80. 1997
    ....
  44. ncbi request reprint Molecular cloning and expression of a human chondroitin synthase
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 276:38721-6. 2001
    ..Thus, we demonstrated that analogous to human heparan sulfate polymerases, the single polypeptide chondroitin synthase possesses two glycosyltransferase activities required for chain polymerization...
  45. ncbi request reprint N-acetylgalactosamine (GalNAc) transfer to the common carbohydrate-protein linkage region of sulfated glycosaminoglycans. Identification of UDP-GalNAc:chondro-oligosaccharide alpha-N-acetylgalactosaminyltransferase in fetal bovine serum
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Japan
    J Biol Chem 270:22190-5. 1995
    ..These results suggest that this novel serum enzyme is a UDP-GalNAc:chondro-oligosaccharide alpha 1-3- or 1-4-N-acetylgalactosaminyltransferase. The possibility of involvement of this enzyme in glycosaminoglycan biosynthesis is discussed...
  46. ncbi request reprint Microanalysis of glycosaminoglycan-derived disaccharides labeled with the fluorophore 2-aminoacridone by capillary electrophoresis and high-performance liquid chromatography
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Japan
    Anal Biochem 232:114-21. 1995
    ....
  47. ncbi request reprint Functional expression and genomic structure of human chondroitin 6-sulfotransferase
    K Tsutsumi
    Department of Biochemistry, Kobe Pharmaceutical University, Japan
    FEBS Lett 441:235-41. 1998
    ..The human C6ST gene spans more than 20 kb and consists of three exons. The protein-coding domain of the C6ST gene is divided into two discrete exons...
  48. ncbi request reprint The tumor suppressor EXT-like gene EXTL2 encodes an alpha1, 4-N-acetylhexosaminyltransferase that transfers N-acetylgalactosamine and N-acetylglucosamine to the common glycosaminoglycan-protein linkage region. The key enzyme for the chain initiation of he
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 274:13933-7. 1999
    ....
  49. ncbi request reprint Molecular cloning and expression of a novel chondroitin 6-O-sulfotransferase
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 275:21075-80. 2000
    ....
  50. ncbi request reprint rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate
    H Kitagawa
    Department of Biochemistry, Kobe Pharmaceutical University, Higashinada ku, Kobe 658 8558, Japan
    J Biol Chem 276:4834-8. 2001
    ..These findings suggest that the biosynthetic mechanism of heparan sulfate in C. elegans is distinct from that reported for the mammalian system...
  51. ncbi request reprint [Systematic analysis of glycome functions of the nematode Caenorhabditis elegans]
    Kazuya Nomura
    Tanpakushitsu Kakusan Koso 48:1057-63. 2003
  52. ncbi request reprint Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans
    Souhei Mizuguchi
    Department of Biology, Faculty of Sciences 33, Kyushu University, Fukuoka 812 8581, Japan
    Nature 423:443-8. 2003
    ..Our findings show that chondroitin is required for embryonic cytokinesis and cell division...
  53. ncbi request reprint [Proteoglycan core glycosyltransferases]
    Toru Uyama
    Tanpakushitsu Kakusan Koso 48:1019-26. 2003
  54. ncbi request reprint Crystal structure of an alpha 1,4-N-acetylhexosaminyltransferase (EXTL2), a member of the exostosin gene family involved in heparan sulfate biosynthesis
    Lars C Pedersen
    Pharmacogenetics Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institute of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 278:14420-8. 2003
    ..These structures represent the first structures from the exostosin gene family and provide important insight into the mechanisms of alpha1,4-N-acetylhexosaminyl transfer in heparan biosynthesis...
  55. pmc Loss of chondroitin 6-O-sulfotransferase-1 function results in severe human chondrodysplasia with progressive spinal involvement
    Holger Thiele
    Institute of Medical Genetics, Charite University Hospital, Humboldt University, Berlin 13353, Germany
    Proc Natl Acad Sci U S A 101:10155-60. 2004
    ..Our results indicate that the mutation in CHST3 described here causes a specific but generalized defect of CS chain sulfation resulting in chondrodysplasia with major involvement of the spine...
  56. ncbi request reprint Chondroitin sulfate characterized by the E-disaccharide unit is a potent inhibitor of herpes simplex virus infectivity and provides the virus binding sites on gro2C cells
    Kicki Bergefall
    Department of Clinical Virology, Goteborg University, Sweden
    J Biol Chem 280:32193-9. 2005
    ..Knowledge of the determinants of antiviral properties of CS-E will help in the development of inhibitors of herpes simplex virus infections in humans...
  57. ncbi request reprint Essential roles of 3'-phosphoadenosine 5'-phosphosulfate synthase in embryonic and larval development of the nematode Caenorhabditis elegans
    Katsufumi Dejima
    Department of Biology, Faculty of Sciences 33, Kyushu University, Fukuoka 812 8581, Japan
    J Biol Chem 281:11431-40. 2006
    ..elegans. Furthermore, reporter analysis showed that pps-1 is expressed in the epidermis and several gland cells but not in neurons and muscles, indicating that PAPS in the neurons and muscles is provided by other cells...
  58. ncbi request reprint Differentiation of epithelial cells to M cells in response to bacterial colonization on the follicle-associated epithelium of Peyer's patch in rat small intestine
    Keigi Chin
    Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Japan
    J Vet Med Sci 68:1023-8. 2006
    ..The uptake of bacteria by M cells might contribute the regulation of the development of indigenous bacterial colonies in the small intestine...
  59. ncbi request reprint Age-related change and its sex differences in histoarchitecture of the hypothalamic suprachiasmatic nucleus of F344/N rats
    Shinji Tsukahara
    Graduate School of Science and Technology, Kobe University, Nada Ku, Kobe 657 8501, Japan
    Exp Gerontol 40:147-55. 2005
    ..However, there was no significant increase in the density of the SCN in adult males. These results suggest that morphological changes in neuronal and astroglial cells occur in the SCN with aging in a sex-specific manner...
  60. ncbi request reprint The cellular differentiation of M cells from crypt undifferentiated epithelial cells into microvillous epithelial cells in follicle-associated epithelia of chicken cecal tonsils
    Hiroshi Kitagawa
    Department of Life Science, Graduate School of Science and Technology, Kobe University, Japan
    J Vet Med Sci 65:171-8. 2003
    ..These findings suggest that M cells are directly derived from the undifferentiated crypt epithelial cells, not fall into apoptotic cell death and further differentiate into MV in the FAE of chicken cecal tonsils...
  61. ncbi request reprint Progression of the dose-related effects of estrogenic endocrine disruptors, an important factor in declining fertility, differs between the hypothalamo-pituitary axis and reproductive organs of male mice
    Katsuhiko Warita
    Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe, Japan
    J Vet Med Sci 68:1257-67. 2006
    ..This finding suggests that endocrine disruptors have an important pronounced effect on StAR gene expression...
  62. ncbi request reprint C. elegans pharyngeal morphogenesis requires both de novo synthesis of pyrimidines and synthesis of heparan sulfate proteoglycans
    Dawn M Franks
    Department of Biological Sciences and the Laboratory for Molecular Biology, University of Illinois at Chicago, 900 S Ashland Avenue, Chicago, IL 60607, USA
    Dev Biol 296:409-20. 2006
    ..We hypothesize that HSPGs are necessary for pharyngeal isthmus elongation, and pyr-1 functions upstream of proteoglycan synthesizing enzymes by providing precursors of UDP-sugars essential for HSPG synthesis...
  63. ncbi request reprint Apoptosis induced by an alkylated purine, 6-dimethylaminopurine, and changes in gene expression in human lymphoma U937 cells
    Masao Nakashima
    Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
    Anticancer Res 28:609-20. 2008
    ....
  64. ncbi request reprint The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus
    Keiki Sugimoto
    Department of Respiratory Medicine, The University of Tokyo Hospital, Tokyo, Japan
    Biochem Biophys Res Commun 359:497-502. 2007
    ..The elevated level of histone methylation resulted in the downregulation of the p16(Ink4a) gene. These results suggest PIWIL4 plays important roles in the chromatin-modifying pathway in human somatic cells...
  65. ncbi request reprint [Chondroitin sugars in embryonic cell division of the nematode C. elegans]
    Souhei Mizuguchi
    Tanpakushitsu Kakusan Koso 49:141-7. 2004
  66. ncbi request reprint Ultrastructural study on the differentiation and the fate of M cells in follicle-associated epithelium of rat Peyer's patch
    Sachiko Onishi
    Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe, Japan
    J Vet Med Sci 69:501-8. 2007
    ....
  67. ncbi request reprint Immunohistochemical study on the delayed progression of epithelial apoptosis in follicle-associated epithelium of rat Peyer's patch
    Sachiko Onishi
    Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    J Vet Med Sci 69:1123-9. 2007
    ....
  68. ncbi request reprint Postnatal apoptosis, development, and sex difference in the lateral septum of rats
    Shinji Tsukahara
    Graduate School of Science and Technology, Kobe University, Nada Ku, Kobe 657 8501, Japan
    J Comp Neurol 475:177-87. 2004
    ..They also indicate that sex difference in caspase-3-independent apoptosis contributes to morphological sexual differentiation of the LSi...