Tomohisa Hasunuma

Summary

Affiliation: Kobe University
Country: Japan

Publications

  1. doi request reprint Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan Electronic address
    J Biosci Bioeng 117:165-9. 2014
  2. pmc Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    J Exp Bot 64:2943-54. 2013
  3. doi request reprint Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells
    Takatoshi Sakamoto
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan
    J Biotechnol 158:203-10. 2012
  4. doi request reprint Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:2597-607. 2013
  5. doi request reprint Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Kobe 657 8501, Japan
    J Biotechnol 163:50-60. 2013
  6. doi request reprint Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 90:997-1004. 2011
  7. doi request reprint Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains
    Hiroko Kato
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 94:1585-92. 2012
  8. doi request reprint Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural
    Keisuke Fujitomi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 111:161-6. 2012
  9. doi request reprint Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae
    Hiroyuki Suga
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 97:1669-78. 2013
  10. doi request reprint Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 135:403-9. 2013

Collaborators

  • Akihiko Kondo
  • Fumio Matsuda
  • Yoshihiro Izumi
  • Jo Shu Chang
  • Takashi Osanai
  • Chikahiro Miyake
  • Masanori Yamasaki
  • Ryosuke Yamada
  • Yuki Matano
  • Hiroko Kato
  • Ku Syahidah Ku Ismail
  • Shimpei Aikawa
  • Takatoshi Sakamoto
  • Shih Hsin Ho
  • Kengo Sasaki
  • Jun Ishii
  • Tomoya Sanda
  • Ancy Joseph
  • Akihito Nakanishi
  • Hiroshi Teramura
  • Hiroyuki Suga
  • Keisuke Fujitomi
  • Hiroyuki Morita
  • Masami Yokota Hirai
  • Chun Yen Chen
  • Tomokazu Shirai
  • Yuri Sakihama
  • Chiaki Ogino
  • Kento Nagata
  • Daisuke Sasaki
  • Kazuya Yoshimura
  • Haruyo Hatanaka
  • Hiroaki Suyama
  • Yoshimi Hori
  • Roumiana Tsenkova
  • Maria Vassileva

Detail Information

Publications30

  1. doi request reprint Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan Electronic address
    J Biosci Bioeng 117:165-9. 2014
    ..The co-expression of TAL/ADH genes is one crucial strategy to fully utilize undetoxified lignocellulosic hydrolysate, leading to cost-competitive ethanol production. ..
  2. pmc Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    J Exp Bot 64:2943-54. 2013
    ..This dynamic metabolic profiling approach provided conclusive evidence of temporal alterations in the metabolic profile in cyanobacterial cells...
  3. doi request reprint Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells
    Takatoshi Sakamoto
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan
    J Biotechnol 158:203-10. 2012
    ..41g/g, which corresponded to 82% of the theoretical yield. The cell surface-engineered strain was thus highly effective for consolidating the process of ethanol production from hemicellulosic materials...
  4. doi request reprint Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:2597-607. 2013
    ....
  5. doi request reprint Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Kobe 657 8501, Japan
    J Biotechnol 163:50-60. 2013
    ..This report provides further transcriptomics information in the interest of producing a robust microorganism for high-temperature ethanol production utilizing xylose...
  6. doi request reprint Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 90:997-1004. 2011
    ..The fermentation profile also indicated that the production of xylitol and glycerol, major by-products in xylose fermentation, was not affected by the upregulation of FDH activity...
  7. doi request reprint Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains
    Hiroko Kato
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 94:1585-92. 2012
    ..8). Thus, the simple hybridization technique facilitated an increase in the xylose fermentation activity...
  8. doi request reprint Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural
    Keisuke Fujitomi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 111:161-6. 2012
    ..Together, our findings demonstrate that PHO13 deletion is a simple, but effective, approach for improving cellulosic bioethanol production by S. cerevisiae...
  9. doi request reprint Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae
    Hiroyuki Suga
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 97:1669-78. 2013
    ..However, poor ethanol yield as well as increased production of xylitol was observed. These results demonstrate that the transhydrogenase function implemented in S. cerevisiae can regulate the redox state of yeast cells...
  10. doi request reprint Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 135:403-9. 2013
    ..Fermentation ability of the recombinant strain was successfully kept during a five-cycle repeated batch fermentation with 86.3% of theoretical yield based on starting biomass...
  11. doi request reprint Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids
    Tomoya Sanda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 102:7917-24. 2011
    ..Long-term stability of ethanol production in the fermentation phase was not only attributed to the coexpression of TAL and FDH genes, but also the hybridization of haploid strains...
  12. doi request reprint Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:2231-7. 2013
    ..5 g/L, one quarter of the cellulose remaining using the wild-type strain, a result of the alleviation of irreversible adsorption of cellulases on the crystalline cellulose...
  13. doi request reprint A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 135:513-22. 2013
    ..This article reviews recent advances in the development of microorganisms for the production of renewable chemicals and advanced biofuels, as well as ethanol, from lignocellulosic materials through consolidated bioprocessing...
  14. ncbi request reprint Variation in biomass properties among rice diverse cultivars
    Fumio Matsuda
    Organization of Advanced Science and Technology, Kobe University, Kobe, Hyogo, Japan
    Biosci Biotechnol Biochem 75:1603-5. 2011
    ..The ethanol productivity from rice straws was evaluated employing a laboratory-scale method based on dilute acid-hydrolysis pretreatment. The results indicated significant variation in biomass properties among the cultivars...
  15. doi request reprint Development of microbial cell factories for bio-refinery through synthetic bioengineering
    Akihiko Kondo
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    J Biotechnol 163:204-16. 2013
    ....
  16. doi request reprint Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply
    Shimpei Aikawa
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Kobe 657 8501, Japan
    Bioresour Technol 108:211-5. 2012
    ..The outcome of this work supports A. platensis as a promising carbohydrate source for biorefinery...
  17. doi request reprint Widely targeted metabolic profiling analysis of yeast central metabolites
    Hiroko Kato
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    J Biosci Bioeng 113:665-73. 2012
    ....
  18. doi request reprint Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen
    Yoshihiro Izumi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nadaku, Kobe 657 8501, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 930:90-7. 2013
    ....
  19. doi request reprint Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803
    Ancy Joseph
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    FEBS Lett 588:466-71. 2014
    ..Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation. ..
  20. doi request reprint Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 108:128-33. 2012
    ..This study demonstrates that cellulases displayed on the yeast cell surface are capable of hydrolyzing cellulose that was not hydrolyzed by commercial cellulases, leading to increased sugar utilization for improved ethanol production...
  21. doi request reprint Near infrared spectroscopy as high-throughput technology for screening of xylose-fermenting recombinant Saccharomyces cerevisiae strains
    Hiroyuki Morita
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Anal Chem 83:4023-9. 2011
    ..21-1.49 g/L of standard error of prediction with calibration, prediction, limit of detection and limit of quantification in the range of 1.0-4.5 and 3.0-13.4 g/L, respectively...
  22. doi request reprint Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Biotechnol Adv 30:1207-18. 2012
    ....
  23. doi request reprint Cocktail δ-integration of xylose assimilation genes for efficient ethanol production from xylose in Saccharomyces cerevisiae
    Hiroko Kato
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    J Biosci Bioeng 116:333-6. 2013
    ..Efficient strains with efficient ethanol production from xylose were successfully obtained by the fermentation test. ..
  24. doi request reprint Development of lipid productivities under different CO2 conditions of marine microalgae Chlamydomonas sp. JSC4
    Akihito Nakanishi
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Bioresour Technol 152:247-52. 2014
    ..This study demonstrated that a newly isolated marine green alga Chlamydomonas sp. JSC4 would be a feasible oil producer due to its high biomass production and lipid productivity under marine salinity. ..
  25. doi request reprint Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration
    Kengo Sasaki
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Bioresour Technol 147:84-8. 2013
    ..cerevisiae was double (5.34-6.44 g L(-1)) that compared with fermentation of liquid hydrolysate before membrane separation (2.75 g L(-1))...
  26. doi request reprint Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe, 657 8501, Japan
    J Ind Microbiol Biotechnol 40:1039-50. 2013
    ..We also speculate that the strong relationship between high temperature and increased xylitol accumulation represents the cell's mechanism to protect itself from heat degradation...
  27. pmc Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Microb Cell Fact 10:2. 2011
    ..Thus, enhanced basic research is expected to identify target genes for improved weak acid tolerance...
  28. doi request reprint Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing
    Ryosuke Yamada
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Biotechnol Adv 31:754-63. 2013
    ..Recent developments in improvement of enzyme productivity by microorganisms and in improvement of the specific activity of cellulase are emphasized. ..
  29. doi request reprint Engineering strategies for improving the CO2 fixation and carbohydrate productivity of Scenedesmus obliquus CNW-N used for bioethanol fermentation
    Shih Hsin Ho
    Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan
    Bioresour Technol 143:163-71. 2013
    ..Therefore, using the proposed carbohydrate-rich microalgal biomass both as the carbon sink and as the feedstock provides a feasible alternative to current carbon-reduction and bioethanol-production strategies...
  30. doi request reprint Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plants
    Tomohisa Hasunuma
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan
    Methods Mol Biol 643:213-27. 2010
    ....