Tomohisa Hasunuma

Summary

Affiliation: Kobe University
Country: Japan

Publications

  1. doi request reprint Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicel
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Microb Cell Fact 13:145. 2014
  2. doi request reprint Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan Electronic address
    J Biosci Bioeng 117:165-9. 2014
  3. pmc Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    J Exp Bot 64:2943-54. 2013
  4. doi request reprint Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells
    Takatoshi Sakamoto
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan
    J Biotechnol 158:203-10. 2012
  5. doi request reprint Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:2597-607. 2013
  6. doi request reprint Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Kobe 657 8501, Japan
    J Biotechnol 163:50-60. 2013
  7. doi request reprint Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe, 657 8501, Japan
    J Ind Microbiol Biotechnol 40:1039-50. 2013
  8. doi request reprint Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural
    Keisuke Fujitomi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 111:161-6. 2012
  9. doi request reprint Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids
    Tomoya Sanda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 102:7917-24. 2011
  10. doi request reprint Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes
    Shuhei Yanase
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 88:381-8. 2010

Collaborators

  • Akihiko Kondo
  • Fumio Matsuda
  • Yoshihiro Izumi
  • Jo Shu Chang
  • Chikahiro Miyake
  • Naoki Takaya
  • Takashi Sazuka
  • Prihardi Kahar
  • Takashi Osanai
  • Masanori Yamasaki
  • Ryosuke Yamada
  • Yota Tsuge
  • Kengo Sasaki
  • Shih Hsin Ho
  • Ku Syahidah Ku Ismail
  • Takatoshi Sakamoto
  • Chiaki Ogino
  • Yuki Matano
  • Hiroko Kato
  • Jun Ishii
  • Kiyotaka Y Hara
  • Yuri Sakihama
  • Hiroshi Teramura
  • Shimpei Aikawa
  • Shuhei Yanase
  • Kentaro Inokuma
  • Xiaoting Ye
  • Daisuke Sasaki
  • Tomoya Sanda
  • Hideo Kawaguchi
  • Michihiro Araki
  • Akihito Nakanishi
  • Ancy Joseph
  • Ginga Shimakawa
  • Hiroyuki Suga
  • Yoshimi Hori
  • Keisuke Fujitomi
  • Hiroyuki Morita
  • Tsutomu Tanaka
  • Hideki Fukuda
  • Hidemi Kitano
  • Kana Morikawa
  • Shigemitsu Kasuga
  • Ko Hirano
  • Takanobu Yoshida
  • Keiji Nishida
  • Ken ichi Oinuma
  • Kouji Uematsu
  • Satoko Niimi-Nakamura
  • Amane Makino
  • Mami Matsuda
  • Motonori Kudou
  • Masami Yokota Hirai
  • Satoshi Wakai
  • Naoko Okai
  • Xin Qing Zhao
  • Chun Yen Chen
  • Haruyo Hatanaka
  • Kento Nagata
  • Tomokazu Shirai
  • Kazuya Yoshimura
  • Hiroaki Suyama
  • Roumiana Tsenkova
  • Maria Vassileva
  • Shohei Kaneko
  • Hideo Noda

Detail Information

Publications46

  1. doi request reprint Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicel
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Microb Cell Fact 13:145. 2014
    ....
  2. doi request reprint Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan Electronic address
    J Biosci Bioeng 117:165-9. 2014
    ..The co-expression of TAL/ADH genes is one crucial strategy to fully utilize undetoxified lignocellulosic hydrolysate, leading to cost-competitive ethanol production. ..
  3. pmc Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    J Exp Bot 64:2943-54. 2013
    ..This dynamic metabolic profiling approach provided conclusive evidence of temporal alterations in the metabolic profile in cyanobacterial cells...
  4. doi request reprint Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells
    Takatoshi Sakamoto
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe 657 8501, Japan
    J Biotechnol 158:203-10. 2012
    ..41g/g, which corresponded to 82% of the theoretical yield. The cell surface-engineered strain was thus highly effective for consolidating the process of ethanol production from hemicellulosic materials...
  5. doi request reprint Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway
    Jun Ishii
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:2597-607. 2013
    ....
  6. doi request reprint Gene expression cross-profiling in genetically modified industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Kobe 657 8501, Japan
    J Biotechnol 163:50-60. 2013
    ..This report provides further transcriptomics information in the interest of producing a robust microorganism for high-temperature ethanol production utilizing xylose...
  7. doi request reprint Time-based comparative transcriptomics in engineered xylose-utilizing Saccharomyces cerevisiae identifies temperature-responsive genes during ethanol production
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai cho, Nada, Kobe, 657 8501, Japan
    J Ind Microbiol Biotechnol 40:1039-50. 2013
    ..We also speculate that the strong relationship between high temperature and increased xylitol accumulation represents the cell's mechanism to protect itself from heat degradation...
  8. doi request reprint Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural
    Keisuke Fujitomi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 111:161-6. 2012
    ..Together, our findings demonstrate that PHO13 deletion is a simple, but effective, approach for improving cellulosic bioethanol production by S. cerevisiae...
  9. doi request reprint Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids
    Tomoya Sanda
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 102:7917-24. 2011
    ..Long-term stability of ethanol production in the fermentation phase was not only attributed to the coexpression of TAL and FDH genes, but also the hybridization of haploid strains...
  10. doi request reprint Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes
    Shuhei Yanase
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 88:381-8. 2010
    ..2% of the theoretical yield. This indicates that high-temperature cellulose fermentation to ethanol can be efficiently accomplished using a recombinant K. marxianus strain displaying thermostable cellulolytic enzymes on the cell surface...
  11. doi request reprint Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration
    Kengo Sasaki
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Bioresour Technol 147:84-8. 2013
    ..cerevisiae was double (5.34-6.44 g L(-1)) that compared with fermentation of liquid hydrolysate before membrane separation (2.75 g L(-1))...
  12. ncbi request reprint Variation in biomass properties among rice diverse cultivars
    Fumio Matsuda
    Organization of Advanced Science and Technology, Kobe University, Kobe, Hyogo, Japan
    Biosci Biotechnol Biochem 75:1603-5. 2011
    ..The ethanol productivity from rice straws was evaluated employing a laboratory-scale method based on dilute acid-hydrolysis pretreatment. The results indicated significant variation in biomass properties among the cultivars...
  13. doi request reprint Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing
    Ryosuke Yamada
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Biotechnol Adv 31:754-63. 2013
    ..Recent developments in improvement of enzyme productivity by microorganisms and in improvement of the specific activity of cellulase are emphasized. ..
  14. doi request reprint Improvements in ethanol production from xylose by mating recombinant xylose-fermenting Saccharomyces cerevisiae strains
    Hiroko Kato
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 94:1585-92. 2012
    ..8). Thus, the simple hybridization technique facilitated an increase in the xylose fermentation activity...
  15. doi request reprint Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 90:997-1004. 2011
    ..The fermentation profile also indicated that the production of xylitol and glycerol, major by-products in xylose fermentation, was not affected by the upregulation of FDH activity...
  16. doi request reprint Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae
    Hiroyuki Suga
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Appl Microbiol Biotechnol 97:1669-78. 2013
    ..However, poor ethanol yield as well as increased production of xylitol was observed. These results demonstrate that the transhydrogenase function implemented in S. cerevisiae can regulate the redox state of yeast cells...
  17. pmc Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Microb Cell Fact 10:2. 2011
    ..Thus, enhanced basic research is expected to identify target genes for improved weak acid tolerance...
  18. doi request reprint Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Appl Microbiol Biotechnol 97:2231-7. 2013
    ..5 g/L, one quarter of the cellulose remaining using the wild-type strain, a result of the alleviation of irreversible adsorption of cellulases on the crystalline cellulose...
  19. doi request reprint Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 135:403-9. 2013
    ..Fermentation ability of the recombinant strain was successfully kept during a five-cycle repeated batch fermentation with 86.3% of theoretical yield based on starting biomass...
  20. doi request reprint Cocktail δ-integration of xylose assimilation genes for efficient ethanol production from xylose in Saccharomyces cerevisiae
    Hiroko Kato
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    J Biosci Bioeng 116:333-6. 2013
    ..Efficient strains with efficient ethanol production from xylose were successfully obtained by the fermentation test. ..
  21. doi request reprint Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass
    Yuki Matano
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 108:128-33. 2012
    ..This study demonstrates that cellulases displayed on the yeast cell surface are capable of hydrolyzing cellulose that was not hydrolyzed by commercial cellulases, leading to increased sugar utilization for improved ethanol production...
  22. ncbi request reprint Applications of yeast cell-surface display in bio-refinery
    Akihiko Kondo
    Graduate School of Engineering, Kobe University, Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Recent Pat Biotechnol 4:226-34. 2010
    ..This review gives an insight in to the recent technological developments in the production of bioenergy, i.e, bioethanol using surface engineered yeast...
  23. doi request reprint Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae
    Kengo Sasaki
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 675 8501, Japan
    Bioresour Technol 185:263-8. 2015
    ....
  24. doi request reprint A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Bioresour Technol 135:513-22. 2013
    ..This article reviews recent advances in the development of microorganisms for the production of renewable chemicals and advanced biofuels, as well as ethanol, from lignocellulosic materials through consolidated bioprocessing...
  25. doi request reprint Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae
    Kengo Sasaki
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 675 8501, Japan
    Bioresour Technol 169:380-6. 2014
    ..Following membrane process was employed: nanofiltration (NF) concentration → (dilution → NF concentration) × 2 times → enzymatic hydrolysis (EH) → ultrafiltration (UF) permeation [..
  26. doi request reprint Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803
    Ancy Joseph
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    FEBS Lett 588:466-71. 2014
    ..Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation. ..
  27. doi request reprint Near infrared spectroscopy as high-throughput technology for screening of xylose-fermenting recombinant Saccharomyces cerevisiae strains
    Hiroyuki Morita
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Anal Chem 83:4023-9. 2011
    ..21-1.49 g/L of standard error of prediction with calibration, prediction, limit of detection and limit of quantification in the range of 1.0-4.5 and 3.0-13.4 g/L, respectively...
  28. doi request reprint Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply
    Shimpei Aikawa
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Kobe 657 8501, Japan
    Bioresour Technol 108:211-5. 2012
    ..The outcome of this work supports A. platensis as a promising carbohydrate source for biorefinery...
  29. doi request reprint Development of microbial cell factories for bio-refinery through synthetic bioengineering
    Akihiko Kondo
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    J Biotechnol 163:204-16. 2013
    ....
  30. doi request reprint Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen
    Yoshihiro Izumi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nadaku, Kobe 657 8501, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 930:90-7. 2013
    ....
  31. doi request reprint Widely targeted metabolic profiling analysis of yeast central metabolites
    Hiroko Kato
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada, Kobe 657 8501, Japan
    J Biosci Bioeng 113:665-73. 2012
    ....
  32. doi request reprint Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity
    Xiaoting Ye
    Organization of Advanced Science and Technology, Kobe University, Nada, Kobe, 657 8501, Japan
    Microb Cell Fact 14:56. 2015
    ..To evaluate 45 genes involved in oxidative phosphorylation in Saccharomyces cerevisiae, we aimed to develop a simple and rapid method to measure mitochondrial ATP synthesis...
  33. doi request reprint Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions
    Yota Tsuge
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 98:8675-83. 2014
    ..These results indicate the presence of a single or multiple endogenous enzymes with broad and high affinity for furfural and co-factors in C. glutamicum ATCC13032. ..
  34. doi request reprint Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering
    Tomohisa Hasunuma
    Organization of Advanced Science and Technology, Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Biotechnol Adv 30:1207-18. 2012
    ....
  35. doi request reprint Zinc, magnesium, and calcium ion supplementation confers tolerance to acetic acid stress in industrial Saccharomyces cerevisiae utilizing xylose
    Ku Syahidah Ku Ismail
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan School of Bioprocess Engineering, Universiti Malaysia Perlis, Perlis, Malaysia
    Biotechnol J 9:1519-25. 2014
    ..Also up-regulation of HXT1 and TKL1 might enhance xylose consumption in the presence of acetic acid. Thus, the addition of ionic nutrients is a simple and cost-effective method to improve the acetic acid tolerance of S. cerevisiae. ..
  36. doi request reprint Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae
    Yuri Sakihama
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    J Biosci Bioeng 119:297-302. 2015
    ....
  37. doi request reprint Perspectives on engineering strategies for improving biofuel production from microalgae--a critical review
    Shih Hsin Ho
    Organization of Advanced Science and Technology, Graduate School of Engineering, Kobe University, 1 1 Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Biotechnol Adv 32:1448-59. 2014
    ..The aim of this review is to provide useful information to facilitate development of innovative and feasible operation technologies for effectively increasing the commercial viability of microalgae-based biofuel production. ..
  38. doi request reprint Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse
    Hideo Kawaguchi
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1 1 Rokkodaicho, Nada Ku, Kobe 657 8501, Japan
    Bioresour Technol 182:169-78. 2015
    ..These results suggest that gradual enzymatic hydrolysis during SSF enhances PhLA production under glucose limitation and reduces the accumulation of fermentation inhibitors, collectively leading to increased PhLA yield. ..
  39. doi request reprint Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in Synechocystis sp. PCC 6803
    Ginga Shimakawa
    a Faculty of Agriculture, Department of Biological and Environmental Science, Graduate School of Agricultural Science, Kobe University, Kobe, Japan
    Biosci Biotechnol Biochem 78:1997-2007. 2014
    ..These observations indicated that respiration provided the carbon source for regeneration of ribulose 1,5-bisphosphate in order to drive the rapid start of photosynthesis. ..
  40. doi request reprint Development of bio-based fine chemical production through synthetic bioengineering
    Kiyotaka Y Hara
    Organization of Advanced Science and Technology, Kobe University, Nada, Kobe, Japan
    Microb Cell Fact 13:173. 2014
    ..In the present review, we summarize recent studies on bio-based fine chemical production and assess the potential of synthetic bioengineering for further improving their productivity. ..
  41. doi request reprint Development of lipid productivities under different CO2 conditions of marine microalgae Chlamydomonas sp. JSC4
    Akihito Nakanishi
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Bioresour Technol 152:247-52. 2014
    ..This study demonstrated that a newly isolated marine green alga Chlamydomonas sp. JSC4 would be a feasible oil producer due to its high biomass production and lipid productivity under marine salinity. ..
  42. doi request reprint Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plants
    Tomohisa Hasunuma
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan
    Methods Mol Biol 643:213-27. 2010
    ....
  43. doi request reprint Efficient co-displaying and artificial ratio control of α-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains
    Kentaro Inokuma
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    Appl Microbiol Biotechnol 99:1655-63. 2015
    ..This strain yielded 22.5 ± 0.6 g/L of ethanol from 100 g/L of raw starch in 120 h of fermentation. ..
  44. doi request reprint Ethanol production from cellulosic materials using cellulase-expressing yeast
    Shuhei Yanase
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Kobe, Japan
    Biotechnol J 5:449-55. 2010
    ..1 g/L for EG-, CBH-, and BGL-displaying yeast, which was higher than that of EG- and CBH-secreting yeast (1.6 g/L ethanol). Our results show that cell surface display is more suitable for direct ethanol fermentation from cellulose...
  45. doi request reprint Recent advances in the metabolic engineering of Corynebacterium glutamicum for the production of lactate and succinate from renewable resources
    Yota Tsuge
    Organization of Advanced Science and Technology, Kobe University, 1 1 Rokkodai, Nada, Kobe, 657 8501, Japan
    J Ind Microbiol Biotechnol 42:375-89. 2015
    ..Here, we summarize current understanding in this field and recent metabolic engineering efforts to develop C. glutamicum strains that efficiently produce L- and D-lactate, and succinate from renewable resources. ..
  46. doi request reprint Engineering strategies for improving the CO2 fixation and carbohydrate productivity of Scenedesmus obliquus CNW-N used for bioethanol fermentation
    Shih Hsin Ho
    Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan
    Bioresour Technol 143:163-71. 2013
    ..Therefore, using the proposed carbohydrate-rich microalgal biomass both as the carbon sink and as the feedstock provides a feasible alternative to current carbon-reduction and bioethanol-production strategies...