H Ashida

Summary

Affiliation: Kobe University
Country: Japan

Publications

  1. doi request reprint Luteolin suppresses TCDD-induced wasting syndrome in a cultured adipocyte model
    Hitoshi Ashida
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan Electronic address
    Pestic Biochem Physiol 120:14-20. 2015
  2. doi request reprint Oolong, black and pu-erh tea suppresses adiposity in mice via activation of AMP-activated protein kinase
    Yoko Yamashita
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada Ku, Kobe 657 8501, Japan
    Food Funct 5:2420-9. 2014
  3. doi request reprint Ashitaba (Angelica keiskei) extract prevents adiposity in high-fat diet-fed C57BL/6 mice
    Tianshun Zhang
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Food Funct 6:135-45. 2015
  4. doi request reprint Chalcones suppress fatty acid-induced lipid accumulation through a LKB1/AMPK signaling pathway in HepG2 cells
    Tianshun Zhang
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Food Funct 5:1134-41. 2014
  5. pmc Ameliorative effects of polyunsaturated fatty acids against palmitic acid-induced insulin resistance in L6 skeletal muscle cells
    Keisuke Sawada
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657 8501, Japan
    Lipids Health Dis 11:36. 2012
  6. ncbi request reprint An update on the dietary ligands of the AhR
    Hitoshi Ashida
    Kobe University, Graduate School of Agricultural Science, Department of Agrobioscience, 1 1 Rokkodai cho, Kobe 657 8501, Nada Ku, Japan
    Expert Opin Drug Metab Toxicol 4:1429-47. 2008
  7. ncbi request reprint Anti-obesity actions of green tea: possible involvements in modulation of the glucose uptake system and suppression of the adipogenesis-related transcription factors
    Hitoshi Ashida
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Biofactors 22:135-40. 2004
  8. ncbi request reprint The heterocyclic amine, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole induces apoptosis in cocultures of rat parenchymal and nonparenchymal liver cells
    H Ashida
    Department of Biofunctional Chemistry, Division of Life Science, Graduate School of Science and Technology, Kobe University, Rokkodai cho 1, Nada Ku, Kobe 657 8501, Japan
    Toxicol Appl Pharmacol 177:59-67. 2001
  9. ncbi request reprint Detection of biomarkers for apoptosis in rat liver after perfusion with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1)
    H Ashida
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biosci Biotechnol Biochem 64:2021-4. 2000
  10. ncbi request reprint Effects of dietary lipid peroxidation products on hormonal responses in primary cultured hepatocytes of rats
    H Ashida
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biosci Biotechnol Biochem 61:2089-94. 1997

Collaborators

Detail Information

Publications71

  1. doi request reprint Luteolin suppresses TCDD-induced wasting syndrome in a cultured adipocyte model
    Hitoshi Ashida
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan Electronic address
    Pestic Biochem Physiol 120:14-20. 2015
    ..Moreover, luteolin was rapidly incorporated and accumulated in 3T3-L1 adipocytes. Thus, luteolin is an attractive compound for the prevention of TCDD-induced wasting syndrome. ..
  2. doi request reprint Oolong, black and pu-erh tea suppresses adiposity in mice via activation of AMP-activated protein kinase
    Yoko Yamashita
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada Ku, Kobe 657 8501, Japan
    Food Funct 5:2420-9. 2014
    ..Our results indicate that oolong, black and pu-erh tea, and in particular, black tea, suppresses adiposity via phosphorylation of the key metabolic regulator AMPK and increases browning of WAT. ..
  3. doi request reprint Ashitaba (Angelica keiskei) extract prevents adiposity in high-fat diet-fed C57BL/6 mice
    Tianshun Zhang
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Food Funct 6:135-45. 2015
    ..In conclusion, Ashitaba extract can possibly prevent adiposity through modulating lipid metabolism through phosphorylation of AMPK in adipose tissue and liver. ..
  4. doi request reprint Chalcones suppress fatty acid-induced lipid accumulation through a LKB1/AMPK signaling pathway in HepG2 cells
    Tianshun Zhang
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Food Funct 5:1134-41. 2014
    ..Collectively, we found that 4HD, XAG, CAR, and XAG attenuated lipid accumulation through activation of the LKB1/AMPK signaling pathway in HepG2 cells...
  5. pmc Ameliorative effects of polyunsaturated fatty acids against palmitic acid-induced insulin resistance in L6 skeletal muscle cells
    Keisuke Sawada
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657 8501, Japan
    Lipids Health Dis 11:36. 2012
    ..There is an increasing demand for compounds including drugs and functional foods that can prevent myocellular insulin resistance...
  6. ncbi request reprint An update on the dietary ligands of the AhR
    Hitoshi Ashida
    Kobe University, Graduate School of Agricultural Science, Department of Agrobioscience, 1 1 Rokkodai cho, Kobe 657 8501, Nada Ku, Japan
    Expert Opin Drug Metab Toxicol 4:1429-47. 2008
    ..Exposure to these environmental contaminants occurs mainly through diet. Recent articles demonstrated that certain food factors regulate the AhR transformation and expression of downstream drug-metabolizing enzymes...
  7. ncbi request reprint Anti-obesity actions of green tea: possible involvements in modulation of the glucose uptake system and suppression of the adipogenesis-related transcription factors
    Hitoshi Ashida
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Biofactors 22:135-40. 2004
    ....
  8. ncbi request reprint The heterocyclic amine, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole induces apoptosis in cocultures of rat parenchymal and nonparenchymal liver cells
    H Ashida
    Department of Biofunctional Chemistry, Division of Life Science, Graduate School of Science and Technology, Kobe University, Rokkodai cho 1, Nada Ku, Kobe 657 8501, Japan
    Toxicol Appl Pharmacol 177:59-67. 2001
    ..These results led to the conclusion that Trp-P-1 mainly drives the caspase-8-mediated pathway that involves Bid, accompanied by a delay in the p53/NF-kappa B-mediated side pathway that involves Bax, Bcl-2, and caspase-9...
  9. ncbi request reprint Detection of biomarkers for apoptosis in rat liver after perfusion with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1)
    H Ashida
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biosci Biotechnol Biochem 64:2021-4. 2000
    ..In addition, Trp-P-1 modulated the DNA binding activity of the apoptosis-related transcription factors, NF-kappaB and AP-1. These results imply a possibility that Trp-P-1 would induce apoptosis in vivo...
  10. ncbi request reprint Effects of dietary lipid peroxidation products on hormonal responses in primary cultured hepatocytes of rats
    H Ashida
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biosci Biotechnol Biochem 61:2089-94. 1997
    ..We conclude that primary cultured hepatocytes from the rats treated in vivo with dietary lipid peroxidation products retained symptoms of oxidative stress and had a low response to glucocorticoids...
  11. ncbi request reprint Tryptophan pyrolysis products, Trp-P-1 and Trp-P-2 induce apoptosis in primary cultured rat hepatocytes
    H Ashida
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biosci Biotechnol Biochem 62:2283-7. 1998
    ..3-Amino-1-methyl-5H-pyrido[4,3-b] indole (Trp-P-2) showed a moderate apoptotic effect, and other compounds had a similar but weaker effect...
  12. ncbi request reprint DNA-damaging carcinogen 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) induces apoptosis via caspase-9 in primary cultured rat hepatocytes
    B Shiotani
    Division of Life Science, Graduate School of Science and Technology and Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Carcinogenesis 22:693-700. 2001
    ..Furthermore, we detected NUC18-like endonuclease activity during apoptosis induced by Trp-P-1. These findings suggest that this apoptosis may have a role against heterocyclic amine-type carcinogens in normal cells...
  13. ncbi request reprint Dietary hydroperoxides of linoleic acid decompose to aldehydes in stomach before being absorbed into the body
    K Kanazawa
    Laboratory of Food and Nutritional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biochim Biophys Acta 1393:349-61. 1998
    ..These results show that the dietary LA-OOH is decomposed to aldehydes in the stomach and that aldehydes are partly absorbed into the body...
  14. doi request reprint Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice
    Rie Mukai
    Department of Agrobioscienec, Graduate School of Agricultural Science, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    J Agric Food Chem 56:10399-405. 2008
    ..CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body...
  15. ncbi request reprint Antimutagenicity of flavones and flavonols to heterocyclic amines by specific and strong inhibition of the cytochrome P450 1A family
    K Kanazawa
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biosci Biotechnol Biochem 62:970-7. 1998
    ..They were also able to act as antimutagens toward other indirect mutagens that were activated by P450 1A1...
  16. ncbi request reprint 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) induces caspase-dependent apoptosis in mononuclear cells
    T Hashimoto
    Division of Life Science, Graduaute School of Science and Technology, Kobe University, Japan
    Biochim Biophys Acta 1539:44-57. 2001
    ..A peptide inhibitor of caspase-8 completely suppressed activation of all other caspases, while an inhibitor of caspase-9 did not. These results indicated that caspase-8 may act as an apical caspase in the Trp-P-1-activated cascade...
  17. ncbi request reprint Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPARgamma2 and C/EBPalpha in 3T3-L1 cells
    Takashi Furuyashiki
    Department of Biofunctional Chemistry, Graduate School of Science and Technology, Kobe University, Japan
    Biosci Biotechnol Biochem 68:2353-9. 2004
    ..Thus, catechin suppressed adipocyte differentiation accompanied by the down-regulation of PPARgamma2, C/EBPalpha, and GLUT4. These results suggest that tea catechin prevents obesity through the suppression of adipocyte differentiation...
  18. ncbi request reprint Inhibition of P-glycoprotein enhances the suppressive effect of kaempferol on transformation of the aryl hydrocarbon receptor
    Rie Mukai
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Japan
    Biosci Biotechnol Biochem 73:1635-9. 2009
    ..In conclusion, inhibition of P-gp enhanced the suppressive effect of kaempferol on AhR transformation through an increase in the intracellular kaempferol concentration...
  19. ncbi request reprint Curcumin suppresses the transformation of an aryl hydrocarbon receptor through its phosphorylation
    Shin Nishiumi
    Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan
    Arch Biochem Biophys 466:267-73. 2007
    ..These results indicate that curcumin is able to bind to the AhR as a ligand, but suppresses its transformation by inhibiting the phosphorylation of AhR and Arnt, probably by PKC...
  20. ncbi request reprint Molokhia (Corchorus olitorius L.) extract suppresses transformation of the aryl hydrocarbon receptor induced by dioxins
    Shin Nishiumi
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Food Chem Toxicol 44:250-60. 2006
    ..These results indicate that molokhia is an attractive food for isolation and identification of a natural antagonist for the AhR...
  21. ncbi request reprint Black tea extract suppresses transformation of aryl hydrocarbon receptor induced by dioxin
    Itsuko Fukuda
    Laboratory of Food and Nutritional Chemistry, Faculty of Agriculture, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Biofactors 21:367-9. 2004
    ..9 microg/ml. The result suggests that intake of black tea has a potential to suppress the AhR transformation, leading protection from dioxin toxicity...
  22. doi request reprint Aryl hydrocarbon receptor-mediated induction of the cytosolic phospholipase A(2)alpha gene by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mouse hepatoma Hepa-1c1c7 cells
    Masaki Kinehara
    Department of Agrobioscience, Kobe University, Rokkodai, Nada Ku, Hyogo, Japan
    J Biosci Bioeng 108:277-81. 2009
    ....
  23. doi request reprint Inhibitory effects of caffeine and its metabolites on intracellular lipid accumulation in murine 3T3-L1 adipocytes
    Hideo Nakabayashi
    Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan
    Biofactors 34:293-302. 2008
    ....
  24. doi request reprint Suppression mechanisms of flavonoids on aryl hydrocarbon receptor-mediated signal transduction
    Rie Mukai
    Department of Agrobioscience, School of Agricultural Science, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Arch Biochem Biophys 501:134-41. 2010
    ..The inhibition of MEK/ERK phosphorylation is one of the mechanisms by which flavanones and catechins suppress the AhR-mediated signal transduction in Hepa-1c1c7 cells...
  25. ncbi request reprint Black tea theaflavins suppress dioxin-induced transformation of the aryl hydrocarbon receptor
    Itsuko Fukuda
    Division of Life Science, Graduate School of Science and Technology, Kobe University, Hyogo, Japan
    Biosci Biotechnol Biochem 69:883-90. 2005
    ..This suggests that theaflavins inhibit the binding of TCDD to the AhR and also the binding of the transformed AhR to the specific DNA-binding site as putative mechanisms...
  26. ncbi request reprint Purification and characterization of a mannose/glucose-specific lectin from Castanea crenata
    K Nomura
    Department of Plant Resource Science, Faculty of Agriculture, Kobe University, Japan
    Phytochemistry 49:667-73. 1998
    ..Some properties of CCA were similar to mannose/glucose-specific legume lectins, but our data suggest that the molecular structure of CCA is different...
  27. doi request reprint Structure-activity relationships of anthraquinones on the suppression of DNA-binding activity of the aryl hydrocarbon receptor induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin
    Itsuko Fukuda
    Research Center for Food Safety and Security, Graduate School of Agricultural Science, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    J Biosci Bioeng 107:296-300. 2009
    ..The findings of this study may be useful for the design of the novel antagonists of the AhR...
  28. ncbi request reprint Effects of combined administration of quercetin, rutin, and extract of white radish sprout rich in kaempferol glycosides on the metabolism in rats
    Takashi Hashimoto
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biosci Biotechnol Biochem 70:279-81. 2006
    ..The combined administration of these flavonoids changed the concentrations of the metabolites in plasma as compared with the concentrations after the administration of a single compound...
  29. ncbi request reprint Interaction between the aryl hydrocarbon receptor and its antagonists, flavonoids
    Itsuko Fukuda
    Research Center for Food Safety and Security, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657 8501, Japan
    Biochem Biophys Res Commun 359:822-7. 2007
    ..These results suggest that flavone, flavonol, and flavanone act as competitive antagonists of the AhR, while catechin associates with the AhRc and indirectly exhibits its antagonistic effects...
  30. doi request reprint Involvement of SREBPs in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced disruption of lipid metabolism in male guinea pig
    Shin Nishiumi
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Toxicol Appl Pharmacol 229:281-9. 2008
    ....
  31. pmc Genetic modification of Bacillus subtilis for production of D-chiro-inositol, an investigational drug candidate for treatment of type 2 diabetes and polycystic ovary syndrome
    Ken ichi Yoshida
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, 1 1 Rokkodai, Kobe, Hyogo 657 8501, Japan
    Appl Environ Microbiol 72:1310-5. 2006
    ..The mutant was able to convert at least 6% of input MI in the culture medium to DCI...
  32. doi request reprint myo-Inositol catabolism in Bacillus subtilis
    Ken ichi Yoshida
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University Kobe 657 8501, Japan
    J Biol Chem 283:10415-24. 2008
    ..The net result of the inositol catabolic pathway in B. subtilis is, thus, the conversion of myo-inositol to an equimolar mixture of dihydroxyacetone phosphate, acetyl-CoA, and CO(2)...
  33. ncbi request reprint Identification of a functional 2-keto-myo-inositol dehydratase gene of Sinorhizobium fredii USDA191 required for myo-inositol utilization
    Ken ichi Yoshida
    Department of Biofunctional Chemistry, Kobe University
    Biosci Biotechnol Biochem 70:2957-64. 2006
    ..USDA191 iolE partially complemented a B. subtilis iolE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myo-inositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and iolE...
  34. ncbi request reprint Suppression of cytochrome P450 1A1 expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mouse hepatoma hepa-1c1c7 cells treated with serum of (-)-epigallocatechin-3-gallate- and green tea extract-administered rats
    Itsuko Fukuda
    Research Center for Food Safety and Security, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan
    Biosci Biotechnol Biochem 73:1206-8. 2009
    ..In Hepa-1c1c7 cells, 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced CYP1A1 expression was suppressed by treatment with the rat serum. It is concluded that catechins can possibly modulate CYP1A1 expression...
  35. ncbi request reprint Anthocyan does not suppress transformation of aryl hydrocarbon receptor induced by dioxin
    Rie Mukai
    Laboratory of Food and Nutritional Chemistry, Faculty of Agriculture, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Biofactors 21:371-3. 2004
    ..All the compounds tested here at 5 microM unexpectedly failed to suppress the transformation induced by 0.1 nM TCDD, indicating that anthocyan does not have a potential to prevent dioxin toxicity...
  36. ncbi request reprint Pigments in green tea leaves (Camellia sinensis) suppress transformation of the aryl hydrocarbon receptor induced by dioxin
    Itsuko Fukuda
    Laboratory of Food and Nutritional Chemistry, Faculty of Agriculture, Kobe University, Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    J Agric Food Chem 52:2499-506. 2004
    ..7 microM. Thus, these pigments of green tea leaves have the potential to protect from dioxin toxicity through the suppression of AhR transformation...
  37. doi request reprint Identification of two scyllo-inositol dehydrogenases in Bacillus subtilis
    Tetsuro Morinaga
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Microbiology 156:1538-46. 2010
    ..The physiological role of IolW remains unclear, but it may be capable of producing SI from SIS with NADPH oxidation...
  38. doi request reprint 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs an insulin signaling pathway through the induction of tumor necrosis factor-alpha in adipocytes
    Shin Nishiumi
    Division of Gastroenterology, Department of Internal Medicine, Graduate School of Medicine, Kobe University, Kobe, Hyogo 650 0017, Japan
    Toxicol Sci 115:482-91. 2010
    ....
  39. ncbi request reprint Comparison in metabolic activity of cytochrome P450 1A1 on heterocyclic amines between human and rat
    K Kanazawa
    Laboratory of Food and Nutritional Chemistry, Faculty of Agriculture, Kobe University, Rokkodai, Nada Ku, Kobe 657 8501, Japan
    J Agric Food Chem 47:4956-61. 1999
    ..The two enzymes also had the same preference for the tested procarcinogens and the same affinities to the specific inhibitors such as flavonoids...
  40. ncbi request reprint Suppressive effect of polysaccharides from the edible and medicinal mushrooms, Lentinus edodes and Agaricus blazei, on the expression of cytochrome P450s in mice
    Takashi Hashimoto
    Division of Life Science, Graduate School of Science and Technology, Kobe University, Japan
    Biosci Biotechnol Biochem 66:1610-4. 2002
    ..Lentinan and ABPS suppressed both the constitutive and 3-methylcholanthrene-induced CYP1A expression and ethoxyresorufin-O-deethylation activity in the liver...
  41. ncbi request reprint Antagonistic and agonistic effects of indigoids on the transformation of an aryl hydrocarbon receptor
    Shin Nishiumi
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Arch Biochem Biophys 470:187-99. 2008
    ..These results indicate that indigoids are able to bind to the AhR as ligands and exhibit antagonistic effects at lower concentrations in mammalian cells...
  42. ncbi request reprint Differential substrate specificity of two inositol transporters of Bacillus subtilis
    Tetsuro Morinaga
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Japan
    Biosci Biotechnol Biochem 74:1312-4. 2010
    ..Conversely to IolT, IolF transported D-chiro-inositol more preferentially than myo-inositol. These results indicate that IolT and IolF are different in substrate specificity...
  43. doi request reprint Epigallocatechin gallate promotes GLUT4 translocation in skeletal muscle
    Manabu Ueda
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Japan
    Biochem Biophys Res Commun 377:286-90. 2008
    ..Therefore, EGCg may improve hyperglycemia by promoting GLUT4 translocation in skeletal muscle with partially different mechanism from insulin...
  44. ncbi request reprint Simultaneous determination of all polyphenols in vegetables, fruits, and teas
    Hiroyuki Sakakibara
    Department of Life Science, Graduate School of Science and Technology, Kobe University, Rokkodai, Nada Ku, Kobe 657 8501, Japan
    J Agric Food Chem 51:571-81. 2003
    ..The present method offers accuracy by avoiding the decomposition of polyphenols during hydrolysis, the ability to determine aglycons separately from glycosides, and information on simple polyphenol levels simultaneously...
  45. ncbi request reprint Screening of indigenous plants from Japan for modulating effects on transformation of the aryl hydrocarbon receptor
    Shin Nishiumi
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University Japan
    Asian Pac J Cancer Prev 7:208-20. 2006
    ..These results suggest that a large variety of plants in Japan contain various compounds modulating, mainly suppressing, AhR transformation...
  46. ncbi request reprint Fucogalactan isolated from Sarcodon aspratus elicits release of tumor necrosis factor-alpha and nitric oxide from murine macrophages
    M Mizuno
    Graduate School of Science and Technology, Kobe University, Japan
    Immunopharmacology 46:113-21. 2000
    ..3-fold. Also, fucogalactan showed dose dependence at concentrations from 5 to 500 microg/ml in NO production. Thus, fucogalactan does elicit the release of cytokines such as TNF-alpha and NO...
  47. ncbi request reprint Catabolic fate of dietary trilinoleoylglycerol hydroperoxides in rat gastrointestines
    K Kanazawa
    Laboratory of Food and Nutritional Chemistry, Faculty of Agriculture, Kobe University, Japan
    Biochim Biophys Acta 1393:336-48. 1998
    ..Thus, dietary TL-OOH was broken down in the stomach releasing, LA-OOH which decomposed further, and did not reach the intestines...
  48. ncbi request reprint High-throughput evaluation of aryl hydrocarbon receptor-binding sites selected via chromatin immunoprecipitation-based screening in Hepa-1c1c7 cells stimulated with 2,3,7,8-tetrachlorodibenzo-p-dioxin
    Masaki Kinehara
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Japan
    Genes Genet Syst 83:455-68. 2008
    ....
  49. ncbi request reprint Functional myo-inositol catabolic genes of Bacillus subtilis Natto are involved in depletion of pinitol in Natto (fermented soybean)
    Tetsuro Morinaga
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Hyogo, Japan
    Biosci Biotechnol Biochem 70:1913-20. 2006
    ..These results suggest that inactivating the MI catabolic genes might prevent PI consumption, retaining it in natto for enrichment of possible health-promoting properties...
  50. doi request reprint Transcriptional regulation of the Bacillus subtilis asnH operon and role of the 5'-proximal long sequence triplication in RNA stabilization
    Tetsuro Morinaga
    Department of Agrobioscience, Kobe University, 1 1 Rokkodai, Nada, Kobe 657 8501, Japan
    Microbiology 156:1632-41. 2010
    ..These results suggest that the 5'-region containing the LST segment could function as an mRNA stabilizer that prolongs the lifetime of the transcript to which it is fused...
  51. ncbi request reprint Structure and replication of cryptic plasmids, pMA1 and pMA2, from a unicellular cyanobacterium, Microcystis aeruginosa
    H Tominaga
    Laboratory of Applied Biologicial Science, Faculty of Agriculture, Shimane University, Japan
    Biosci Biotechnol Biochem 59:1217-20. 1995
    ..No rep protein was detected. Replication mechanisms of the plasmids are discussed...
  52. ncbi request reprint Apoptosis in the thymus after intraperitoneal injection of rats with Trp-P-1
    Takashi Hashimoto
    Division of Life Science, Graduate School of Science and Technology, Kobe University, Hyogo, Japan
    Environ Mol Mutagen 40:175-83. 2002
    ..Therefore, Trp-P-1 activates both pro- and anti-apoptotic signals in vivo in the immune system, particularly in the thymus, and the former signal overcomes the latter...
  53. ncbi request reprint Rapid preparation of a plasma membrane fraction from adipocytes and muscle cells: application to detection of translocated glucose transporter 4 on the plasma membrane
    Shin Nishiumi
    Graduate School of Agricultural Science, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    Biosci Biotechnol Biochem 71:2343-6. 2007
    ..It was found that IRbeta and GLUT4 levels on the plasma membrane decreased in adipose and muscle with intake of a 29% lard diet for 14 weeks. Hence, this method should be useful for rapid preparation of the plasma membrane fraction...
  54. ncbi request reprint D-pinitol and myo-inositol stimulate translocation of glucose transporter 4 in skeletal muscle of C57BL/6 mice
    Nhung Thuy Dang
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Japan
    Biosci Biotechnol Biochem 74:1062-7. 2010
    ..PI and MI, therefore, have the potential to prevent diabetes mellitus by reducing the postprandial blood glucose level and stimulating GLUT4 translocation in the skeletal muscle...
  55. ncbi request reprint Anthocyans fail to suppress transformation of aryl hydrocarbon receptor induced by dioxin
    Rie Mukai
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Hyogo, Japan
    Biosci Biotechnol Biochem 69:896-903. 2005
    ..It is concluded that anthocyans are not suitable candidates for protection from dioxin toxicity...
  56. ncbi request reprint 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (trp-P-1) is incorporated into rat splenocytes, thymocytes, and hepatocytes through monoamine transporters and induces apoptosis
    Takashi Hashimoto
    Division of Life Science, Graduate School of Science and Technology, Kobe University, Japan
    Biosci Biotechnol Biochem 66:1205-12. 2002
    ..These results demonstrated that Trp-P-1 is incorporated into the cells through monoamine transporters and induces apoptosis...
  57. ncbi request reprint Rutin-enhanced antibacterial activities of flavonoids against Bacillus cereus and Salmonella enteritidis
    Hidetoshi Arima
    Division of Life Science, Graduate School of Science and Technology, Kobe University, Japan
    Biosci Biotechnol Biochem 66:1009-14. 2002
    ..enteritidis was used as the test bacterium. The MIC value for kaempherol was markedly decreased by the addition of rutin. Morin inhibited DNA synthesis, and this effect was promoted by rutin at a concentration of 25 microg/ml...
  58. doi request reprint Tea catechins modulate the glucose transport system in 3T3-L1 adipocytes
    Manabu Ueda
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe 657 8501, Japan
    Food Funct 1:167-73. 2010
    ..Therefore, EC and EGC promote the translocation of GLUT4 through activation of PI3K, and Cg and EGCg inhibit insulin-induced translocation of GLUT4 by the insulin signaling pathway in 3T3-L1 cells...
  59. ncbi request reprint Decreased expression of Bcl-x protein during hepatocarcinogenesis induced exogenously and endogenously in rats
    Y Hatanaka
    Division of Life Science, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan
    Jpn J Cancer Res 92:1270-7. 2001
    ....
  60. ncbi request reprint Up-regulation of CD13/aminopeptidase N induced by phorbol ester is involved in redox regulation and tumor necrosis factor alpha production in HL-60 cells
    Yutaka Hatanaka
    Division of Life Science, Graduate School of Science and Technology, Kobe University, Japan
    Inflammation 26:175-81. 2002
    ....
  61. ncbi request reprint Evoking cytochrome P450 1A activity interferes with apoptosis induced by 3-amino-1,4-dimethyl-5H-pyrido [4,3-b]indole (Trp-P-1) in rat hepatocytes under the ex vivo system
    Bunsyo Shiotani
    Division of Life Science, Graduate School of Science and Technology, Kobe University, Japan
    Biosci Biotechnol Biochem 66:356-62. 2002
    ..Phenobarbital and pyridine did not affect Trp-P-1-induced apoptosis. These results suggested that evoking CYP 1A activity might interfere with apoptosis induced by Trp-P-1 in rat hepatocytes under the ex vivo system...
  62. ncbi request reprint 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) triggers apoptosis by DNA double-strand breaks caused by inhibition of topoisomerase I
    Bunsyo Shiotani
    Division of Life Science, Graduate School of Science and Technology, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Carcinogenesis 25:1149-55. 2004
    ..These results indicate that Trp-P-1-induced apoptosis was triggered by DNA DSBs through the inhibition of topoisomerase I but not the formation of DNA adducts...
  63. ncbi request reprint Antioxidative bioavailability of artepillin C in Brazilian propolis
    Kazuo Shimizu
    Department of Life Science, Graduate School of Science and Technology, Kobe University, Rokkodai, Nada Ku, Kobe 657 8501, Japan
    Arch Biochem Biophys 424:181-8. 2004
    ..Artepillin C is a bioavailable antioxidant...
  64. ncbi request reprint A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists
    Itsuko Fukuda
    Department of Life Science, Graduate School of Science and Technology, Kobe University, Rokkodai cho, Nada Ku, Kobe, Hyogo 657 8501, Japan
    J Immunol Methods 287:187-201. 2004
    ..These results indicate that SW-ELISA is a new and straightforward method for the detection of AhR transformation and will be useful in screening of agonists or antagonists for AhR...
  65. ncbi request reprint Lentinan from shiitake mushroom (Lentinus edodes) suppresses expression of cytochrome P450 1A subfamily in the mouse liver
    Takashi Okamoto
    Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Rokkodai cho 1, Nada Ku, Kobe 657 8501, Japan
    Biofactors 21:407-9. 2004
    ..This paper discusses on the effective lentinan dosage and route of administration for suppression of CYP1As...
  66. ncbi request reprint Suppression of dioxin mediated aryl hydrocarbon receptor transformation by ethanolic extracts of propolis
    Yong K Park
    State University of Campinas, College of Food Engineering UNICAMP, Department of Food Science, Laboratory of Food Biochemistry, SP, Brazil
    Biosci Biotechnol Biochem 68:935-8. 2004
    ..The IC(50) values of propolis group 3 and group 12 were 1.2 and 3.6 microg/ml, respectively, indicating that propolis showed stronger antagonistic effects as compared with vegetable extracts...
  67. ncbi request reprint Suppressive effects of ethanolic extracts from propolis and its main botanical origin on dioxin toxicity
    Yong K Park
    Department of Food Science, College of Food Engineering, State University of Campinas, P O Box 6177, Campinas, SP, Brazil
    J Agric Food Chem 53:10306-9. 2005
    ..These results indicate that not only propolis but also its botanical origin contains high amounts of flavonoid aglycones and that both of them are useful dietary sources for flavonoids with a potency to prevent dioxin toxicity...
  68. ncbi request reprint Cyanidin 3-glucoside ameliorates hyperglycemia and insulin sensitivity due to downregulation of retinol binding protein 4 expression in diabetic mice
    Rie Sasaki
    College of Bioscience and Biotechnology, Chubu University, Matsumoto cho, Kasugai, Aichi 487 8501, Japan
    Biochem Pharmacol 74:1619-27. 2007
    ..These findings indicate that C3G has significant potency in an anti-diabetic effect through the regulation of Glut4-RBP4 system and the related inflammatory adipocytokines...
  69. ncbi request reprint TCDD-induced CYP1A1 expression, an index of dioxin toxicity, is suppressed by flavonoids permeating the human intestinal Caco-2 cell monolayers
    Mika Hamada
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo ku, 113 8657, Japan
    J Agric Food Chem 54:8891-8. 2006
    ..It is proposed from these results that some flavonoids have the ability to suppress dioxin-induced AhR activity after permeating the human intestinal epithelial cell monolayer...
  70. ncbi request reprint N-acetyl-L-cysteine suppresses constitutive expression of CD11a/LFA-1alpha protein in myeloid lineage
    Kaoru Hashizume
    Department of Immunohistochemistry, DAKO Japan Company Ltd, Kakkyoyama 18, Nishinotoin Higashiiru, Shijo dori, Simogyo, Kyoto, Japan
    Leuk Res 26:939-44. 2002
    ....
  71. doi request reprint Multitargeted cancer prevention by quercetin
    Akira Murakami
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Cancer Lett 269:315-25. 2008
    ..Dietary quercetin is, therefore, a promising agent for cancer prevention and further research is warranted...