T Fukamizo

Summary

Affiliation: Kinki University
Country: Japan

Publications

  1. ncbi request reprint Chitosanase from Streptomyces sp. strain N174: a comparative review of its structure and function
    T Fukamizo
    Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, Nara, Japan
    Biochem Cell Biol 75:687-96. 1997
  2. ncbi request reprint Binding of a highly de-N-acetylated chitosan to Japanese pheasan lysozyme as measured by 1H-NMR spectroscopy
    T Fukamizo
    Department of Food and Nutrition, Kinki University, Nakamachi, Nara, Japan
    Biosci Biotechnol Biochem 65:1766-73. 2001
  3. ncbi request reprint Chitin-related enzymes in agro-biosciences
    Yasuyuki Arakane
    Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University, Buk Gu, Gwangju, Korea
    Curr Drug Targets 13:442-70. 2012
  4. pmc Novel β-N-acetylglucosaminidases from Vibrio harveyi 650: cloning, expression, enzymatic properties, and subsite identification
    Wipa Suginta
    Biochemistry Electrochemistry Research Unit, School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
    BMC Biochem 11:40. 2010
  5. ncbi request reprint Chitinase from Autographa californica multiple nucleopolyhedrovirus: rapid purification from Sf-9 medium and mode of action
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University
    Biosci Biotechnol Biochem 75:1763-9. 2011
  6. doi request reprint A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds (Hordeum vulgare L.)
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University, Nara, Japan
    Biochim Biophys Acta 1794:1159-67. 2009
  7. doi request reprint Enzymatic hydrolysis of 1,3-1,4-beta-glucosyl oligosaccharides by 1,3-1,4-beta-glucanase from Synechocystis PCC6803: a comparison with assays using polymer and chromophoric oligosaccharide substrates
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Arch Biochem Biophys 478:187-94. 2008
  8. ncbi request reprint Bacillus circulans MH-K1 chitosanase: amino acid residues responsible for substrate binding
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University, 3327 204 Nakamachi, Nara 631 8505
    J Biochem 138:563-9. 2005
  9. ncbi request reprint Theoretical calculation of pKa reveals an important role of Arg205 in the activity and stability of Streptomyces sp. N174 chitosanase
    T Fukamizo
    Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, Nara, Japan
    J Biol Chem 275:25633-40. 2000
  10. ncbi request reprint State of imidazole side chain of hen lysozyme modified with histamine and Japanese quail lysozyme. A study by immobilized metal ion affinity chromatography
    T Fukamizo
    Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, Nara, Japan
    Biosci Biotechnol Biochem 62:2239-41. 1998

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Chitosanase from Streptomyces sp. strain N174: a comparative review of its structure and function
    T Fukamizo
    Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, Nara, Japan
    Biochem Cell Biol 75:687-96. 1997
    ..This information can be helpful for the design of new chitinolytic enzymes that can be applied to carbohydrate engineering, biological control of phytopathogens, and other fields including chitinous polysaccharide degradation...
  2. ncbi request reprint Binding of a highly de-N-acetylated chitosan to Japanese pheasan lysozyme as measured by 1H-NMR spectroscopy
    T Fukamizo
    Department of Food and Nutrition, Kinki University, Nakamachi, Nara, Japan
    Biosci Biotechnol Biochem 65:1766-73. 2001
    ..In addition, the C2H signal of His114 of JPL was not affected by the chitosan binding. These results suggest that the monoacetylated chitosan binds to subsites E and F through the left-sided binding mode...
  3. ncbi request reprint Chitin-related enzymes in agro-biosciences
    Yasuyuki Arakane
    Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University, Buk Gu, Gwangju, Korea
    Curr Drug Targets 13:442-70. 2012
    ..All of these studies have opened the way to efficiently utilize these enzyme tools for enhancing the agricultural productions...
  4. pmc Novel β-N-acetylglucosaminidases from Vibrio harveyi 650: cloning, expression, enzymatic properties, and subsite identification
    Wipa Suginta
    Biochemistry Electrochemistry Research Unit, School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
    BMC Biochem 11:40. 2010
    ..We have previously reported the structure and function of chitinase A from Vibrio harveyi 650. This study t reports the identification of two GlcNAcases from the same organism and their detailed functional characterization...
  5. ncbi request reprint Chitinase from Autographa californica multiple nucleopolyhedrovirus: rapid purification from Sf-9 medium and mode of action
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University
    Biosci Biotechnol Biochem 75:1763-9. 2011
    ..The AcMNPV chitinase processively hydrolyzes solid β-chitin in a manner similar to SmChiA. The processive mechanism of the enzyme appears to be advantageous in liquefaction of infected host larvae...
  6. doi request reprint A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds (Hordeum vulgare L.)
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University, Nara, Japan
    Biochim Biophys Acta 1794:1159-67. 2009
    ..These tryptophan residues are likely to interact with each other, resulting in the non-additivity of mutational effects...
  7. doi request reprint Enzymatic hydrolysis of 1,3-1,4-beta-glucosyl oligosaccharides by 1,3-1,4-beta-glucanase from Synechocystis PCC6803: a comparison with assays using polymer and chromophoric oligosaccharide substrates
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Arch Biochem Biophys 478:187-94. 2008
    ..It is likely that the kinetic data obtained from such chromophoric substrates do not always reflect the true enzymatic properties...
  8. ncbi request reprint Bacillus circulans MH-K1 chitosanase: amino acid residues responsible for substrate binding
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University, 3327 204 Nakamachi, Nara 631 8505
    J Biochem 138:563-9. 2005
    ..Thus, we conclude that the flexible loop containing Lys218 plays an important role in substrate binding, and that the role of Tyr148 is less critical, but still important, due to a stacking interaction or hydrogen bond...
  9. ncbi request reprint Theoretical calculation of pKa reveals an important role of Arg205 in the activity and stability of Streptomyces sp. N174 chitosanase
    T Fukamizo
    Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, Nara, Japan
    J Biol Chem 275:25633-40. 2000
    ....
  10. ncbi request reprint State of imidazole side chain of hen lysozyme modified with histamine and Japanese quail lysozyme. A study by immobilized metal ion affinity chromatography
    T Fukamizo
    Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, Nara, Japan
    Biosci Biotechnol Biochem 62:2239-41. 1998
    ....
  11. ncbi request reprint Chitinolytic enzymes: catalysis, substrate binding, and their application
    T Fukamizo
    Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Curr Protein Pept Sci 1:105-24. 2000
    ..Recent understanding of the catalytic and substrate binding mechanisms would be helpful as well for arrangement of a powerful strategy in such an application...
  12. ncbi request reprint Exchange of nucleoside monophosphate-binding domains in adenylate kinase and UMP/CMP kinase
    T Okajima
    Faculty of Agriculture, Kinki University, Nara, 631 8505, Japan
    J Biochem 124:359-67. 1998
    ....
  13. ncbi request reprint The role of Arg114 at subsites E and F in reactions catalyzed by hen egg-white lysozyme
    Shunsuke Kawamura
    Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto 869 1404, Japan
    Biosci Biotechnol Biochem 72:823-32. 2008
    ..We speculate that such a conformational change causes differences in substrate and acceptor binding at subsites E and F, lowering the efficiency of glycosyl transfer reaction of lysozyme...
  14. ncbi request reprint Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues, sugar recognition specificity, kinetics, and synergism
    Tamo Fukamizo
    Department of Advanced Bioscience, Kinki University, 3327 204 Nakamichi, Nara 631 8505, Japan
    Glycobiology 16:1064-72. 2006
    ..N174 endochitosanase, the highest synergy in the rate of product formation was observed at the molar ratio 2:1. Thus, GlcNase would be an efficient tool for industrial production of glucosamine monosaccharide...
  15. ncbi request reprint Rice chitinases: sugar recognition specificities of the individual subsites
    Chiye Sasaki
    Department of Advanced Bioscience, Kinki University, Nakamachi, Nara, Japan
    Glycobiology 16:1242-50. 2006
    ....
  16. doi request reprint Oligosaccharide hydrolysis by chitosanase enzymes monitored by real-time electrospray ionization-mass spectrometry
    Nicole Dennhart
    Analytical Research Group, Department for Basic Life Sciences, Technische Universitat Munchen, Weihenstephaner Berg 3, 85354 Freising Weihenstephan, Germany
    J Biotechnol 134:253-60. 2008
    ..N174 endochitosanase wild type and of two of its mutants. This technique establishes the fast and efficient determination of in vitro enzymatic activities of various enzyme systems...
  17. ncbi request reprint Role of acidic amino acid residues in chitooligosaccharide-binding to Streptomyces sp. N174 chitosanase
    Tomomi Katsumi
    Department of Advanced Bioscience, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Biochem Biophys Res Commun 338:1839-44. 2005
    ..The fluorescence quenching was very weak in D57A, suggesting a strong contribution of this residue to the oligosaccharide binding...
  18. ncbi request reprint Comparative study of the reaction mechanism of family 18 chitinases from plants and microbes
    Chiye Sasaki
    Laboratory of Enzyme System Science, Department of Food and Nutrition, Kinki University, Nakamachi, Nara 631 8505, Japan
    J Biochem 131:557-64. 2002
    ..Minor structural differences between plant and microbial enzymes appear to result in such functional variations, even though all of these chitinases are classified into the identical family of glycosyl hydrolases...
  19. ncbi request reprint Site-directed mutagenesis and functional analysis of an active site tryptophan of insect chitinase
    Hong Zhang
    Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
    Insect Biochem Mol Biol 32:1477-88. 2002
    ..The variations in kcat's among the mutated enzymes and the IC50 for the transition state inhibitor analog, allosamidin, indicate that W145 also influences formation of the transition state during catalysis...
  20. ncbi request reprint Family 19 chitinase from Aeromonas sp. No.10S-24: role of chitin-binding domain in the enzymatic activity
    Miki Kojima
    Laboratories of Enzyme System Science, Faculty of Agriculture, Kinki University, Nara 631 8505
    J Biochem 137:235-42. 2005
    ..The truncation of ChBDs was found to strongly affect the affinity at the (-1) site. This situation seems to result in the lower enzymatic activity of the ChBD-truncated enzyme toward the chitinous substrates...
  21. ncbi request reprint Family 19 chitinase from rice (Oryza sativa L.): substrate-binding subsites demonstrated by kinetic and molecular modeling studies
    Chiye Sasaki
    Laboratory of Enzyme System Science, Department of Food and Nutrition, Kinki University, 3327 204, Naka machi, Nara 631 8505, Japan
    Plant Mol Biol 52:43-52. 2003
    ....
  22. ncbi request reprint A novel type of family 19 chitinase from Aeromonas sp. No.10S-24. Cloning, sequence, expression, and the enzymatic properties
    Mitsuhiro Ueda
    Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan
    Eur J Biochem 270:2513-20. 2003
    ..Thus, the Aeromonas enzyme was found to be a novel type of family 19 chitinase in its structural and functional properties...
  23. ncbi request reprint Novel chitosanase from Streptomyces griseus HUT 6037 with transglycosylation activity
    Toshiaki Tanabe
    Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Saga 840 8502, Japan
    Biosci Biotechnol Biochem 67:354-64. 2003
    ..The deduced primary structure was similar to endoglucanase E-5 of Thermomonospora fusca, which enzyme belongs to family 5 of the glycosyl hydrolases. This is the first report of a family 5 chitosanase with transglycosylation activity...
  24. pmc Beta-1,4-glucanase-like protein from the cyanobacterium Synechocystis PCC6803 is a beta-1,3-1,4-glucanase and functions in salt stress tolerance
    Masahiro Tamoi
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Biochem J 405:139-46. 2007
    ..Under osmotic stress (100-400 mM sorbitol), there was no difference in growth between the wild-type and the Ssglc-disrupted mutant cells. These results suggest that SsGlc functions in salt stress tolerance in Synechocystis PCC6803...
  25. doi request reprint Role of the loop structure of the catalytic domain in rice class I chitinase
    Ryoji Mizuno
    Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata, Japan
    J Biochem 143:487-95. 2008
    ..Thus, we concluded that loop II is involved in sugar residue binding at the +3 subsite, but that Trp159 itself appears to contribute only partly to sugar residue interaction at the subsite...
  26. ncbi request reprint Chitosan-soyprotein interaction as determined by thermal unfolding experiments
    Tomoko Takeuchi
    Department of Advanced Bioscience, Kinki University, Nara 631 8505, Japan
    Biosci Biotechnol Biochem 70:1786-9. 2006
    ..We conclude that chitosan interacts with beta-conglycinin through electrostatic interactions between the positive charges of the chitosan polysaccharide and the negative charges of the protein surface...
  27. pmc Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases
    Nathalie Cote
    Centre d étude et de valorisation de la diversité microbienne, Departement de Biologie, Faculte des Sciences, Universite de Sherbrooke, Sherbrooke, QC, Canada, J1K 2R1
    Biochem J 394:675-86. 2006
    ..Alignments of several other GH2 members allowed the identification of yet another putative subfamily, characterized by a novel, serine-glutamate doublet at these positions...
  28. ncbi request reprint The Calvin cycle in cyanobacteria is regulated by CP12 via the NAD(H)/NADP(H) ratio under light/dark conditions
    Masahiro Tamoi
    Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Nakamachi, Nara 631 8505, Japan
    Plant J 42:504-13. 2005
    ..These data suggest that under light and dark conditions, the oligomerization of CP12 with PRK and GAPDH regulates the activities of both enzymes and thus the carbon flow from the Calvin cycle to the oxidative pentose phosphate cycle...
  29. ncbi request reprint Biospecific fractionation of chitosan
    Chiye Sasaki
    Laboratory of Enzyme System Science, Department of Food and Nutrition, Kinki University, 3327 204 Nakamachi, Nara 631 8505 Japan
    Biomacromolecules 4:1686-90. 2003
    ....
  30. ncbi request reprint Disruption of the gene encoding the ChiB1 chitinase of Aspergillus fumigatus and characterization of a recombinant gene product
    Alex K Jaques
    Division of Microbiology, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    Microbiology 149:2931-9. 2003
    ..fumigatus TIGR database (http://www.tigr.org/)...
  31. ncbi request reprint Kinetic studies on the hydrolysis of N-acetylated and N-deacetylated derivatives of 4-methylumbelliferyl chitobioside by the family 18 chitinases ChiA and ChiB from Serratia marcescens
    Yuji Honda
    National Food Research Institute, 2 1 12, Kannondai, Tsukuba, Ibaraki 305 8642, Japan
    J Biochem 133:253-8. 2003
    ..The reaction mechanism of family 18 chitinases is discussed based upon the results obtained from the hydrolysis of these compounds...
  32. ncbi request reprint 26 kDa endochitinase from barley seeds: an interaction of the ionizable side chains essential for catalysis
    Tsuneo Ohnishi
    Department of Advanced Bioscience, Kinki University, 3327 204 Nakamachi, Nara, 631 8505
    J Biochem 138:553-62. 2005
    ..N174 [Fukamizo et al. (2000) J. Biol. Chem. 275, 25633-25640]; hence, this type of interaction seems to be at least partly conserved in the catalytic cleft of other glycosyl hydrolases...