Haruhiko Siomi

Summary

Affiliation: Keio University
Country: Japan

Publications

  1. pmc Is canalization more than just a beautiful idea?
    Kaoru Sato
    Department of Molecular Biology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    Genome Biol 11:109. 2010
  2. ncbi request reprint Posttranscriptional regulation of microRNA biogenesis in animals
    Haruhiko Siomi
    Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Cell 38:323-32. 2010
  3. ncbi request reprint Transposable elements, RNA silencing, and their impacts on the genome throughout evolution
    Haruhiko Siomi
    Department of Molecular Biology Keio University School of Medicine, Tokyo, Japan
    Uirusu 58:55-60. 2008
  4. pmc Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila
    Kuniaki Saito
    Keio University School of Medicine, Tokyo, Japan
    Genes Dev 24:2493-8. 2010
  5. pmc Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis
    Akihiro Nagao
    Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
    RNA 16:2503-15. 2010
  6. ncbi request reprint A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila
    Kuniaki Saito
    Keio University School of Medicine, Tokyo 160 8582, Japan
    Nature 461:1296-9. 2009
  7. pmc Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in Drosophila
    Keita Miyoshi
    Keio University School of Medicine, Tokyo 160 8582, Japan
    RNA 16:506-15. 2010
  8. pmc Maelstrom coordinates microtubule organization during Drosophila oogenesis through interaction with components of the MTOC
    Kaoru Sato
    Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
    Genes Dev 25:2361-73. 2011
  9. ncbi request reprint Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila
    Kazumichi M Nishida
    Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Mol Cell 49:680-91. 2013
  10. doi request reprint A direct role for Hsp90 in pre-RISC formation in Drosophila
    Tomohiro Miyoshi
    Keio University School of Medicine, Tokyo, Japan
    Nat Struct Mol Biol 17:1024-6. 2010

Collaborators

Detail Information

Publications26

  1. pmc Is canalization more than just a beautiful idea?
    Kaoru Sato
    Department of Molecular Biology, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    Genome Biol 11:109. 2010
    ..A new study suggests that the buffering function of Hsp90 could instead be due to its repression of de novo transposon-mediated mutagenesis...
  2. ncbi request reprint Posttranscriptional regulation of microRNA biogenesis in animals
    Haruhiko Siomi
    Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Cell 38:323-32. 2010
    ..Here, we discuss recent studies that shed light on how multiple steps in the miRNA biogenesis pathway are regulated to modulate miRNA function in animals...
  3. ncbi request reprint Transposable elements, RNA silencing, and their impacts on the genome throughout evolution
    Haruhiko Siomi
    Department of Molecular Biology Keio University School of Medicine, Tokyo, Japan
    Uirusu 58:55-60. 2008
    ..Complex interactions between TEs and RNA silencing machineries have been co-opted to regulate cellular genes...
  4. pmc Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila
    Kuniaki Saito
    Keio University School of Medicine, Tokyo, Japan
    Genes Dev 24:2493-8. 2010
    ..Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing...
  5. pmc Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis
    Akihiro Nagao
    Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
    RNA 16:2503-15. 2010
    ..These findings suggest that the impacts of armi mutants on the operation of the piRNA pathway are variable in germ cells of fly testes...
  6. ncbi request reprint A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila
    Kuniaki Saito
    Keio University School of Medicine, Tokyo 160 8582, Japan
    Nature 461:1296-9. 2009
    ..Thus, in gonadal somatic cells, tj gives rise simultaneously to two different molecules: the TJ protein, which activates Piwi expression, and piRNAs, which define the Piwi targets for silencing...
  7. pmc Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in Drosophila
    Keita Miyoshi
    Keio University School of Medicine, Tokyo 160 8582, Japan
    RNA 16:506-15. 2010
    ..These results suggest that the precursor RNA structure determines the biogenesis mechanism of esiRNAs and miRNAs, thereby implicating hairpin structures with long stems as intermediates in the evolution of Drosophila miRNA...
  8. pmc Maelstrom coordinates microtubule organization during Drosophila oogenesis through interaction with components of the MTOC
    Kaoru Sato
    Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
    Genes Dev 25:2361-73. 2011
    ..These findings suggest a model in which Mael serves as a platform that nucleates other MTOC components to form a functional MTOC in early oocyte development, which is independent of Chk2 activation and DNA damage signaling...
  9. ncbi request reprint Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila
    Kazumichi M Nishida
    Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Mol Cell 49:680-91. 2013
    ..The functions of R2D2 are required to avoid endo-siRNA misdirection to Ago1, because Ago1 is capable of loading incompletely complementary miRNA duplexes and endo-siRNA duplexes...
  10. doi request reprint A direct role for Hsp90 in pre-RISC formation in Drosophila
    Tomohiro Miyoshi
    Keio University School of Medicine, Tokyo, Japan
    Nat Struct Mol Biol 17:1024-6. 2010
    ....
  11. doi request reprint Chapter 16. How to define targets for small guide RNAs in RNA silencing: a biochemical approach
    Mikiko C Siomi
    Keio University School of Medicine, Tokyo, Japan JST, CREST, Saitama, Japan
    Methods Enzymol 449:345-55. 2008
    ..The method discussed is useful for defining targets for the small RNAs that function in RNA silencing...
  12. ncbi request reprint piRNA-mediated silencing in Drosophila germlines
    Mikiko C Siomi
    Keio University School of Medicine, Tokyo 160 8582, Japan
    Semin Cell Dev Biol 21:754-9. 2010
    ....
  13. pmc DmGTSF1 is necessary for Piwi-piRISC-mediated transcriptional transposon silencing in the Drosophila ovary
    Hitoshi Ohtani
    Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Genes Dev 27:1656-61. 2013
    ..We suggest that DmGTSF1, a nuclear Piwi interactor, is an integral factor in Piwi-piRISC-mediated transcriptional silencing. ..
  14. pmc Gender-Specific Hierarchy in Nuage Localization of PIWI-Interacting RNA Factors in Drosophila
    Akihiro Nagao
    Siomi Lab, Department of Molecular Biology, Keio University School of Medicine Tokyo, Japan
    Front Genet 2:55. 2011
    ..This suggests that the functional requirement of Krimp in piRNA biogenesis may be different in male and female gonads...
  15. pmc How does the royal family of Tudor rule the PIWI-interacting RNA pathway?
    Mikiko C Siomi
    Keio University School of Medicine, Tokyo 160 8582, Japan
    Genes Dev 24:636-46. 2010
    ..Although Tud domains in Tud family members are known to be sDMA-binding modules, involvement of the Tudor family at the molecular level in the piRNA pathway has only recently come into focus...
  16. pmc Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlines
    Kazumichi M Nishida
    Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
    EMBO J 28:3820-31. 2009
    ..Thus, in germline cells, piRNAs are quality-controlled by dPRMT5 that modifies PIWI proteins, in tight association with Tud...
  17. doi request reprint Many ways to generate microRNA-like small RNAs: non-canonical pathways for microRNA production
    Keita Miyoshi
    Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Genet Genomics 284:95-103. 2010
    ....
  18. doi request reprint Biochemical analyzes of endogenous argonaute complexes immunopurified with anti-Argonaute monoclonal antibodies
    Keita Miyoshi
    Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
    Methods Mol Biol 725:29-43. 2011
    ..Furthermore, we will also explain how various parameters, such as antibody properties and buffer conditions, can alter the production and interpretation of experimental data...
  19. pmc Characterization of the miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathway
    Keita Miyoshi
    School of Medicine, Keio University, Tokyo 160 8582, Japan
    RNA 15:1282-91. 2009
    ..The AGO1-PIWI mutant showed no Slicer activity but associates with mature miRNA. These results indicate that these domains are required differently for miRLC and miRISC formation in the miRNA pathway...
  20. doi request reprint Purification of dFMR1-containing complexes using tandem affinity purification
    Keita Miyoshi
    Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
    Methods Mol Biol 1010:111-21. 2013
    ..In this chapter, we describe a tandem affinity purification method to isolate the protein components and small RNAs in the dFMR1-associated complexes...
  21. doi request reprint On the road to reading the RNA-interference code
    Haruhiko Siomi
    Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Nature 457:396-404. 2009
    ....
  22. ncbi request reprint Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate
    Takamasa Hirano
    Department of Molecular Biology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    RNA 20:1223-37. 2014
    ....
  23. ncbi request reprint Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly
    Yukiko Murota
    Institute for Genome Research, University of Tokushima, Tokushima 770 8503, Japan Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Cell Rep 8:103-13. 2014
    ..We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells. ..
  24. doi request reprint Gatekeepers for Piwi-piRNA complexes to enter the nucleus
    Hirotsugu Ishizu
    Department of Molecular Biology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    Curr Opin Genet Dev 21:484-90. 2011
    ..This mechanism involves gatekeepers for a functional Piwi-piRNA complex to be imported, which parallels with the Tetrahymena Twi1p-scan RNA pathway used to carry out the programmed DNA elimination...
  25. doi request reprint How selfish retrotransposons are silenced in Drosophila germline and somatic cells
    Mikiko C Siomi
    Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo, Japan
    FEBS Lett 582:2473-8. 2008
    ..In this review, we address RNA silencing mechanisms that silence retrotransposons, a subset of TEs, and discuss how germline and somatic cells are equipped with different retrotransposon silencing mechanisms...
  26. ncbi request reprint Stress signaling etches heritable marks on chromatin
    Haruhiko Siomi
    Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Cell 145:1005-7. 2011
    ..Seong et al. (2011) now reveal how stress-induced signaling through dATF-2 disrupts heterochromatin and leaves heritable marks that influence patterns of gene expression in subsequent generations...