Hideyuki Okano

Summary

Affiliation: Keio University
Country: Japan

Publications

  1. ncbi request reprint Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum
    Toru Yamashita
    Bridgestone Laboratory of Developmental and Regenerative Neurobiology, Keio University School of Medicine, Shinanomachi, Tokyo 160 8582, Japan
    J Neurosci 26:6627-36. 2006
  2. ncbi request reprint The first clinical trial in Tohoku University Hospital after the Great East Japan Earthquake: the heroic efforts of my friend, Professor Masashi Aoki
    Hideyuki Okano
    Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    Keio J Med 61:3-9. 2012
  3. pmc Beneficial compaction of spinal cord lesion by migrating astrocytes through glycogen synthase kinase-3 inhibition
    Francois Renault-Mihara
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    EMBO Mol Med 3:682-96. 2011
  4. pmc The semaphorin 3A inhibitor SM-345431 accelerates peripheral nerve regeneration and sensitivity in a murine corneal transplantation model
    Masahiro Omoto
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 7:e47716. 2012
  5. pmc RNA-binding protein Musashi1 modulates glioma cell growth through the post-transcriptional regulation of Notch and PI3 kinase/Akt signaling pathways
    Jun Muto
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    PLoS ONE 7:e33431. 2012
  6. pmc Prospective isolation of murine and human bone marrow mesenchymal stem cells based on surface markers
    Yo Mabuchi
    Department of Physiology, Keio University School of Medicine, Shinanomachi 35, Shinjuku ku, Tokyo 160 8582, Japan Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Yushima 1 5 45, Bunkyo ku, Tokyo 113 8510, Japan
    Stem Cells Int 2013:507301. 2013
  7. pmc MicroRNAs in Neural Stem Cells and Neurogenesis
    Hironori Kawahara
    Department of Physiology, Keio University School of Medicine Shinjuku, Tokyo, Japan
    Front Neurosci 6:30. 2012
  8. pmc iPS cell technologies: significance and applications to CNS regeneration and disease
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Brain 7:22. 2014
  9. pmc Reprogramming non-human primate somatic cells into functional neuronal cells by defined factors
    Zhi Zhou
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Brain 7:24. 2014
  10. pmc Inflammatory cascades mediate synapse elimination in spinal cord compression
    Morito Takano
    Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Neuroinflammation 11:40. 2014

Collaborators

Detail Information

Publications108 found, 100 shown here

  1. ncbi request reprint Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum
    Toru Yamashita
    Bridgestone Laboratory of Developmental and Regenerative Neurobiology, Keio University School of Medicine, Shinanomachi, Tokyo 160 8582, Japan
    J Neurosci 26:6627-36. 2006
    ..These results highlight the role of the SVZ in neuronal regeneration after a stroke and its potential as an important therapeutic target for various neurological disorders...
  2. ncbi request reprint The first clinical trial in Tohoku University Hospital after the Great East Japan Earthquake: the heroic efforts of my friend, Professor Masashi Aoki
    Hideyuki Okano
    Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    Keio J Med 61:3-9. 2012
    ..Thanks to his strong leadership and support from many reliable colleagues, we could finally start new clinical trials for ALS patients. In this article, I wish to introduce Professor Aoki's heroic efforts...
  3. pmc Beneficial compaction of spinal cord lesion by migrating astrocytes through glycogen synthase kinase-3 inhibition
    Francois Renault-Mihara
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    EMBO Mol Med 3:682-96. 2011
    ..Our results reveal a novel benefit of GSK-3 inhibition for SCI and suggest that the stimulation of astrocyte migration is a feasible therapeutic strategy for traumatic injury in the central nervous system...
  4. pmc The semaphorin 3A inhibitor SM-345431 accelerates peripheral nerve regeneration and sensitivity in a murine corneal transplantation model
    Masahiro Omoto
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 7:e47716. 2012
    ..We examined the efficacy of the semaphorin 3A inhibitor, SM-345431, in promoting regeneration of peripheral nerves in a mouse corneal transplantation model...
  5. pmc RNA-binding protein Musashi1 modulates glioma cell growth through the post-transcriptional regulation of Notch and PI3 kinase/Akt signaling pathways
    Jun Muto
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    PLoS ONE 7:e33431. 2012
    ..These results suggest that MSI1 increases the growth and/or survival of certain types of glioma cells by promoting the activation of both Notch and PI(3) kinase/Akt signaling...
  6. pmc Prospective isolation of murine and human bone marrow mesenchymal stem cells based on surface markers
    Yo Mabuchi
    Department of Physiology, Keio University School of Medicine, Shinanomachi 35, Shinjuku ku, Tokyo 160 8582, Japan Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Yushima 1 5 45, Bunkyo ku, Tokyo 113 8510, Japan
    Stem Cells Int 2013:507301. 2013
    ..The isolation of MSCs using selective markers (combination of PDGFR α and Sca-1) is crucial to address the many unanswered questions pertaining to these cells and has the potential to enhance their therapeutic potential enormously...
  7. pmc MicroRNAs in Neural Stem Cells and Neurogenesis
    Hironori Kawahara
    Department of Physiology, Keio University School of Medicine Shinjuku, Tokyo, Japan
    Front Neurosci 6:30. 2012
    ..Moreover, defects in core proteins involved in miRNA biogenesis including Drosha, DGCR8, and Dicer promote tumorigenesis. Thus, the study of not only mature miRNA function but also miRNA biogenesis steps is likely to be important...
  8. pmc iPS cell technologies: significance and applications to CNS regeneration and disease
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Brain 7:22. 2014
    ....
  9. pmc Reprogramming non-human primate somatic cells into functional neuronal cells by defined factors
    Zhi Zhou
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Brain 7:24. 2014
    ..The aim of the present study was to determine whether direct reprogramming technology was applicable to common marmosets...
  10. pmc Inflammatory cascades mediate synapse elimination in spinal cord compression
    Morito Takano
    Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Neuroinflammation 11:40. 2014
    ....
  11. pmc Rewiring of regenerated axons by combining treadmill training with semaphorin3A inhibition
    Liang Zhang
    Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160 8582, Japan
    Mol Brain 7:14. 2014
    ....
  12. pmc The long non-coding RNA nuclear-enriched abundant transcript 1_2 induces paraspeckle formation in the motor neuron during the early phase of amyotrophic lateral sclerosis
    Yoshinori Nishimoto
    Department of Physiology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Brain 6:31. 2013
    ..The aim of this study was to clarify the interaction of paraspeckles with ALS-associated RNA-binding proteins, and to identify increased occurrence of paraspeckles in the nucleus of ALS spinal motor neurons...
  13. pmc Mitochondrial dysfunction associated with increased oxidative stress and α-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue
    Yoichi Imaizumi
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Tokyo 160 8582, Japan
    Mol Brain 5:35. 2012
    ..The familial form of PD, PARK2, is caused by mutations in the parkin gene. parkin-knockout mouse models show some abnormalities, but they do not fully recapitulate the pathophysiology of human PARK2...
  14. pmc A human Dravet syndrome model from patient induced pluripotent stem cells
    Norimichi Higurashi
    Department of Pediatrics, School of Medicine, Fukuoka University, 45 1, 7 chome, Nanakuma, Jonan ku, Fukuoka 814 0180, Japan
    Mol Brain 6:19. 2013
    ..However, no such effort has been reported to date. We here report a cellular model for DS that utilizes patient-derived iPSCs...
  15. pmc Time-dependent changes in the microenvironment of injured spinal cord affects the therapeutic potential of neural stem cell transplantation for spinal cord injury
    Soraya Nishimura
    Department of Orthopaedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, 160 8582, Japan
    Mol Brain 6:3. 2013
    ..To address this question, NS/PC transplantation was performed after contusive spinal cord injury in adult mice at the sub-acute and chronic phases...
  16. doi request reprint Steps toward safe cell therapy using induced pluripotent stem cells
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160 8582, Japan
    Circ Res 112:523-33. 2013
    ..Insights on safety and preclinical use of iPSCs in cardiovascular repair model are also discussed...
  17. doi request reprint The common marmoset as a novel animal model system for biomedical and neuroscience research applications
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160 8582, Japan
    Semin Fetal Neonatal Med 17:336-40. 2012
    ..In this review, we summarize the recent progress of biomedical research and neuroscience using common marmoset as an excellent model system...
  18. pmc Impaired spatial and contextual memory formation in galectin-1 deficient mice
    Masanori Sakaguchi
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, Ontario, Canada
    Mol Brain 4:33. 2011
    ..Together, these data reveal a role for galectin-carbohydrate signalling in hippocampal memory function...
  19. doi request reprint Strategic approaches to regeneration of a damaged central nervous system
    Hideyuki Okano
    Department of Physiology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo, Japan
    Cornea 30:S15-8. 2011
    ..To achieve regeneration of a damaged CNS, our main strategies encompass: (1) axonal regeneration, (2) replenishment of lost neural cells, and (3) functional recovery. In this review, achievements using these approaches will be outlined...
  20. doi request reprint Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets
    Junichi Yamane
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    J Neurosci Res 88:1394-405. 2010
    ..These findings suggest that Gal-NS/PCs have better therapeutic potential than NS/PCs for SCI in nonhuman primates and that human Gal-NS/PC transplantation might be a feasible treatment for human SCI...
  21. pmc Pre-evaluated safe human iPSC-derived neural stem cells promote functional recovery after spinal cord injury in common marmoset without tumorigenicity
    Yoshiomi Kobayashi
    Department of Orthopedic Surgery, School of Medicine, Keio University, Tokyo, Japan
    PLoS ONE 7:e52787. 2012
    ..Taken together, these results indicate that pre-evaluated safe hiPSC-NS/PCs are a potential source of cells for the treatment of SCI in the clinic...
  22. ncbi request reprint Blockade of interleukin-6 signaling aggravates ischemic cerebral damage in mice: possible involvement of Stat3 activation in the protection of neurons
    Toru Yamashita
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurochem 94:459-68. 2005
    ..These results suggest that endogenous IL-6 plays a critical role in preventing damaged neurons from undergoing apoptosis in the acute phase of cerebral ischemia and that its role may be mediated by Stat3 activation...
  23. pmc Angiotensin II type 1 receptor signaling contributes to synaptophysin degradation and neuronal dysfunction in the diabetic retina
    Toshihide Kurihara
    Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan
    Diabetes 57:2191-8. 2008
    ..The aim of the present study was to show the relationship of the renin-angiotensin system (RAS) with the synaptic vesicle protein synaptophysin and neuronal activity in the diabetic retina...
  24. ncbi request reprint Sox21 promotes hippocampal adult neurogenesis via the transcriptional repression of the Hes5 gene
    Satoru Matsuda
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    J Neurosci 32:12543-57. 2012
    ..Therefore, Sox21 controls hippocampal adult neurogenesis via transcriptional repression of the Hes5 gene...
  25. ncbi request reprint In vivo imaging of engrafted neural stem cells: its application in evaluating the optimal timing of transplantation for spinal cord injury
    Seiji Okada
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    FASEB J 19:1839-41. 2005
    ..Optimization of cell therapies can be greatly accelerated and refined by imaging, and the methods in the present study can be widely applied to various research fields of regeneration medicine, including transplantation study...
  26. ncbi request reprint Chondroitinase ABC combined with neural stem/progenitor cell transplantation enhances graft cell migration and outgrowth of growth-associated protein-43-positive fibers after rat spinal cord injury
    Takeshi Ikegami
    Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
    Eur J Neurosci 22:3036-46. 2005
    ....
  27. pmc Therapeutic potential of appropriately evaluated safe-induced pluripotent stem cells for spinal cord injury
    Osahiko Tsuji
    Department of Physiology, School of Medicine, Keio University, Shinjuku, Tokyo 160 8582, Japan
    Proc Natl Acad Sci U S A 107:12704-9. 2010
    ..These findings suggest that pre-evaluated safe iPS clone-derived neural stem/progenitor cells may be a promising cell source for transplantation therapy for SCI...
  28. ncbi request reprint Galectin-1 regulates neurogenesis in the subventricular zone and promotes functional recovery after stroke
    Satoru Ishibashi
    Department of Neurology and Neurological Science, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8510, Japan
    Exp Neurol 207:302-13. 2007
    ....
  29. pmc Fbxo45, a novel ubiquitin ligase, regulates synaptic activity
    Hirobumi Tada
    Department of Physiology, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Biol Chem 285:3840-9. 2010
    ..We also found that Fbxo45 induces the degradation of a synaptic vesicle-priming factor, Munc13-1. We propose that Fbxo45 plays an important role in the regulation of neurotransmission by modulating Munc13-1 at the synapse...
  30. pmc Schwann-spheres derived from injured peripheral nerves in adult mice--their in vitro characterization and therapeutic potential
    Takehiko Takagi
    Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 6:e21497. 2011
    ....
  31. doi request reprint Evaluation of human fetal neural stem/progenitor cells as a source for cell replacement therapy for neurological disorders: properties and tumorigenicity after long-term in vitro maintenance
    Daisuke Ogawa
    Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    J Neurosci Res 87:307-17. 2009
    ..These findings support the idea that hNS/PCs may represent a nontumorigenic, safe, and appropriate cell source for regenerative therapies for neurological disorders...
  32. pmc Roles of ES cell-derived gliogenic neural stem/progenitor cells in functional recovery after spinal cord injury
    Gentaro Kumagai
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 4:e7706. 2009
    ..These findings suggest that gliogenic NS/PCs are effective for promoting the recovery from SCI, and provide essential insight into the mechanisms through which cellular transplantation leads to functional improvement after SCI...
  33. doi request reprint Neural crest-derived stem cells migrate and differentiate into cardiomyocytes after myocardial infarction
    Yuichi Tamura
    Department of Cardiology, Keio University School of Medicine, Tokyo, Japan
    Arterioscler Thromb Vasc Biol 31:582-9. 2011
    ..Here, we examined the migration and differentiation potential of these cells on myocardial infarction (MI)...
  34. ncbi request reprint Prospective characterization of neural stem cells by flow cytometry analysis using a combination of surface markers
    Masako Nagato
    Department of Pediatrics, Faculty of Medicine, Kyoto University, Kyoto, Japan
    J Neurosci Res 80:456-66. 2005
    ..These results indicate the usefulness of syndecan-1, Notch-1, and integrin-beta1 as NSC markers...
  35. doi request reprint Significance of remyelination by neural stem/progenitor cells transplanted into the injured spinal cord
    Akimasa Yasuda
    Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo, Japan
    Stem Cells 29:1983-94. 2011
    ..These findings establish the biological importance of remyelination by graft-derived cells for functional recovery after the transplantation of NS/PCs into the injured spinal cord...
  36. pmc Grafted human-induced pluripotent stem-cell-derived neurospheres promote motor functional recovery after spinal cord injury in mice
    Satoshi Nori
    Department of Physiology, School of Medicine, Keio University, Shinjuku, Tokyo 160 8582, Japan
    Proc Natl Acad Sci U S A 108:16825-30. 2011
    ..No tumor formation was observed in the hiPSC-NS-grafted mice. These findings suggest that hiPSCs give rise to neural stem/progenitor cells that support improved function post-SCI and are a promising cell source for its treatment...
  37. ncbi request reprint Comparison between fetal spinal-cord- and forebrain-derived neural stem/progenitor cells as a source of transplantation for spinal cord injury
    Kota Watanabe
    Department of Orthopaedic Surgery, Keio University, Tokyo, Japan
    Dev Neurosci 26:275-87. 2004
    ..In conclusion, forebrain-derived NSPCs could be used as an alternative to spinal-cord-derived NSPCs as a potential therapeutic agent for spinal cord injuries...
  38. pmc FRS2α regulates Erk levels to control a self-renewal target Hes1 and proliferation of FGF-responsive neural stem/progenitor cells
    Takuya Sato
    Division of Systems Biomedical Technology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Stem Cells 28:1661-73. 2010
    ..Thus, FRS2α fine-tunes the FGF-signaling to control qualitatively different biological activities, self-renewal at least partly through Hes1 versus proliferation of NSPCs...
  39. doi request reprint Anti-IL-6-receptor antibody promotes repair of spinal cord injury by inducing microglia-dominant inflammation
    Masahiko Mukaino
    Department of Rehabilitation Medicine, Keio University School of Medicine, Shinjuku, Tokyo 160 8582, Japan
    Exp Neurol 224:403-14. 2010
    ..Our findings indicate that the functional improvement elicited by MR16-1 involves microglial functions, and provide new insights into the role of IL-6 signaling in the pathology of SCI...
  40. ncbi request reprint Environmental cue-dependent dopaminergic neuronal differentiation and functional effect of grafted neuroepithelial stem cells in parkinsonian brain
    Yutaka Mine
    Department of Neurosurgery, School of Medicine, Keio University, Tokyo, Japan
    Neurosurgery 65:741-53; discussion 753. 2009
    ....
  41. ncbi request reprint A selective Sema3A inhibitor enhances regenerative responses and functional recovery of the injured spinal cord
    Shinjiro Kaneko
    Department of Orthopaedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku ku, Tokyo 160 8582, Japan
    Nat Med 12:1380-9. 2006
    ..Thus, Sema3A is essential for the inhibition of axonal regeneration and other regenerative responses after spinal cord injury (SCI). These results support the possibility of using Sema3A inhibitors in the treatment of human SCI...
  42. ncbi request reprint Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery
    Yuji Mikami
    Neuroimmunology Research Group, Keio University School of Medicine, Tokyo, Japan
    J Neurosci Res 76:453-65. 2004
    ..Our results suggest that DC-implantation exerts trophic effects, including activation of endogenous NSPCs, leading to repair of the injured adult spinal cord...
  43. doi request reprint Efficient generation of mature cerebellar Purkinje cells from mouse embryonic stem cells
    Osamu Tao
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurosci Res 88:234-47. 2010
    ..Thus, EDPC precursors in SFEB can mature into PCs whose properties are comparable with those of native PCs in vitro...
  44. doi request reprint Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression
    Ken ichiro Kuwako
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Neuron 67:407-21. 2010
    ..Together, these findings indicate that Msi1-mediated posttranscriptional regulation of Robo3 controls midline crossing of precerebellar neurons...
  45. pmc Human hepatocyte growth factor promotes functional recovery in primates after spinal cord injury
    Kazuya Kitamura
    Department of Orthopedic Surgery, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    PLoS ONE 6:e27706. 2011
    ..In addition, this is the first presentation of a novel scale for assessing neurological motor performance in non-human primates after contusive cervical SCI...
  46. pmc Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow
    Satoru Morikawa
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    J Exp Med 206:2483-96. 2009
    ..Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities...
  47. pmc Stem cell-like properties of the endometrial side population: implication in endometrial regeneration
    Hirotaka Masuda
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 5:e10387. 2010
    ..The objective of this study was to isolate and characterize putative endometrial stem/progenitor cells and to address how they might be involved in the physiology of endometrium...
  48. pmc Efficient derivation of multipotent neural stem/progenitor cells from non-human primate embryonic stem cells
    Hiroko Shimada
    Department of Orthopedic Surgery, School of Medicine, Keio University, Tokyo, Japan
    PLoS ONE 7:e49469. 2012
    ....
  49. ncbi request reprint Potential functional neural repair with grafted neural stem cells of early embryonic neuroepithelial origin
    Koichi Uchida
    Department of Neurosurgery, School of Medicine, Keio University, Tokyo 160 8582, Japan
    Neurosci Res 52:276-86. 2005
    ..Neuroepithelial stem cells are therefore an attractive candidate as a source of donor material for intracerebral grafting in functional repair...
  50. ncbi request reprint Macrophage migration inhibitory factor (MIF) promotes cell survival and proliferation of neural stem/progenitor cells
    Shigeki Ohta
    Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Shinjuku ku, Japan
    J Cell Sci 125:3210-20. 2012
    ....
  51. ncbi request reprint SOCS3 is required to temporally fine-tune photoreceptor cell differentiation
    Yoko Ozawa
    Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Dev Biol 303:591-600. 2007
    ..Thus, SOCS3 can act as a temporal fine-tuner of STAT3 activation during photoreceptor cell differentiation...
  52. pmc Purified mesenchymal stem cells are an efficient source for iPS cell induction
    Kunimichi Niibe
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 6:e17610. 2011
    ..In particular, the choice of cell type most suitable for inducing high-quality iPS cells remains unclear...
  53. ncbi request reprint Distinct functions of human numb isoforms revealed by misexpression in the neural stem cell lineage in the Drosophila larval brain
    Masako Toriya
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Dev Neurosci 28:142-55. 2006
    ....
  54. ncbi request reprint Human neural stem/progenitor cells, expanded in long-term neurosphere culture, promote functional recovery after focal ischemia in Mongolian gerbils
    Satoru Ishibashi
    Department of Neurology and Neurological Science, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    J Neurosci Res 78:215-23. 2004
    ..These results indicate that in vitro-expanded human neurosphere cells are a potential source for transplantable material for treatment of stroke...
  55. ncbi request reprint Hepatocyte growth factor promotes endogenous repair and functional recovery after spinal cord injury
    Kazuya Kitamura
    Department of Orthopaedic Surgery, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    J Neurosci Res 85:2332-42. 2007
    ..These results suggest that HGF gene delivery to the injured spinal cord exerts multiple beneficial effects and enhances endogenous repair after SCI. This is the first study to demonstrate the efficacy of HGF for SCI...
  56. ncbi request reprint Visualization of spatiotemporal activation of Notch signaling: live monitoring and significance in neural development
    Jun Kohyama
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Dev Biol 286:311-25. 2005
    ....
  57. pmc Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells
    Satoru Yoshida
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 6:e28856. 2011
    ..However, in order to apply iPS cells to mitotic tissue, induction of tissue stem cells that give rise to progeny of the target organ is required...
  58. ncbi request reprint Conditional ablation of Stat3 or Socs3 discloses a dual role for reactive astrocytes after spinal cord injury
    Seiji Okada
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Nat Med 12:829-34. 2006
    ..These results suggest that Stat3 is a key regulator of reactive astrocytes in the healing process after SCI, providing a potential target for intervention in the treatment of CNS injury...
  59. ncbi request reprint Functional corneal endothelium derived from corneal stroma stem cells of neural crest origin by retinoic acid and Wnt/β-catenin signaling
    Shin Hatou
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
    Stem Cells Dev 22:828-39. 2013
    ..This protocol enables efficient production of corneal endothelium from corneal stromal stem cells by direct induction, which may lead to a novel stem cell therapy for corneal endothelial dysfunction...
  60. doi request reprint Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing
    Narihito Nagoshi
    Department of Physiology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo, Japan
    Glia 59:771-84. 2011
    ..This process, involving Schwann cell plasticity, is a novel repair mechanism for the lesioned mammalian spinal cord...
  61. ncbi request reprint Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation
    Masato Yano
    CREST JST and the Department of Physiology, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Biol Chem 280:12690-9. 2005
    ....
  62. pmc Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation
    Yoko Ozawa
    Department of Ophthalmology, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Biol Chem 283:24561-70. 2008
    ..These findings suggest SOCS3 as a potential therapeutic target molecule for protecting photoreceptor cell function during inflammation...
  63. doi request reprint Derivation of induced pluripotent stem cells by retroviral gene transduction in mammalian species
    Masanori Imamura
    Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    Methods Mol Biol 925:21-48. 2012
    ..The iPSC technology will serve as a valuable method for studying genomic imprinting, and conversely, the insights from these studies will offer valuable criteria to assess the potential of iPSCs...
  64. doi request reprint The formation of an angiogenic astrocyte template is regulated by the neuroretina in a HIF-1-dependent manner
    Ayako Nakamura-Ishizu
    Department of Cell Differentiation, The Sakaguchi Laboratory, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Dev Biol 363:106-14. 2012
    ..Our data demonstrates that the neuroretina but not astrocytes acts as a primary oxygen sensor which ultimately controls the retinal vascular development by regulating an angiogenic astrocyte template...
  65. ncbi request reprint Epidermal growth factor signaling mediated by grb2 associated binder1 is required for the spatiotemporally regulated proliferation of olig2-expressing progenitors in the embryonic spinal cord
    Yoshika Hayakawa-Yano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Stem Cells 25:1410-22. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  66. pmc von Hippel-Lindau protein regulates transition from the fetal to the adult circulatory system in retina
    Toshihide Kurihara
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Development 137:1563-71. 2010
    ..Our results suggest that not only macrophages but also tissue oxygen-sensing mechanisms regulate the transition from the fetal to the adult circulatory system in the retina...
  67. doi request reprint Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells
    Hiromi Kumamaru
    Department of Orthopedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Nat Commun 3:1140. 2012
    ..These results highlight the vulnerability of engrafted NSPCs to environmental force, while emphasizing the importance of in situ analysis in advancing the efficacy and safety of stem cell-based therapies...
  68. doi request reprint In vivo tracing of neural tracts in tiptoe walking Yoshimura mice by diffusion tensor tractography
    Morito Takano
    Department of Orthopaedic Surgery, Keio University, Shinanomachi 35, Shinjuku ku, Tokyo, Japan
    Spine (Phila Pa 1976) 38:E66-72. 2013
    ..Basic imaging experiment...
  69. doi request reprint Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad
    Narihito Nagoshi
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Cell Stem Cell 2:392-403. 2008
    ..The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease...
  70. pmc Transplantation of human neural stem/progenitor cells overexpressing galectin-1 improves functional recovery from focal brain ischemia in the Mongolian gerbil
    Junichi Yamane
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Mol Brain 4:35. 2011
    ..This study provides evidence for a potential application of hGal1 with hNSPCs-transplantation in the treatment of brain ischemia...
  71. doi request reprint Development of mesenchymal stem cells partially originate from the neural crest
    Satoru Morikawa
    Department of Physiology, Keio University School of Medicine, 35 Shinano machi, Shinjuku ku, Tokyo 160 8582, Japan
    Biochem Biophys Res Commun 379:1114-9. 2009
    ..Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest...
  72. ncbi request reprint Establishment of three-dimensional culture of neural stem/progenitor cells in collagen Type-1 Gel
    Kota Watanabe
    Department of Orthopaedic Surgery, Keio University 35 Shinanomachi, Shinjuku, Tokyo 160 8582, Japan
    Restor Neurol Neurosci 25:109-17. 2007
    ..We, then, evaluated the ability of NSPCs to differentiate under the optimal condition...
  73. doi request reprint Musashi1 as a marker of reactive astrocytes after transient focal brain ischemia
    Koichi Oki
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Neurosci Res 66:390-5. 2010
    ..However, the difference in the distributions of Msi1 and Nestin suggests that some of their features may differ in reactive astrocytes...
  74. doi request reprint Stromal cell-secreted factors promote the survival of embryonic stem cell-derived early neural stem/progenitor cells via the activation of MAPK and PI3K-Akt pathways
    Seiji Ishii
    Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    J Neurosci Res 88:722-34. 2010
    ..Thus, PA6CM appears to contain soluble factors that promote the survival of ES cell-derived early NS/PCs through the activation of the MAPK and PI3K-Akt pathways...
  75. doi request reprint Blockade of interleukin-6 signaling suppressed cochlear inflammatory response and improved hearing impairment in noise-damaged mice cochlea
    Kenichiro Wakabayashi
    Department of Otolaryngology, Head and Neck Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinju ku, Tokyo 160 8582, Japan
    Neurosci Res 66:345-52. 2010
    ..Anti-inflammatory cytokine therapy including IL-6 blockade would be a feasible novel therapeutic strategy for acute sensory neural hearing loss...
  76. doi request reprint Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development
    Hayato Naka
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Nat Neurosci 11:1014-23. 2008
    ..These findings reveal a part of the timer mechanisms for generating diverse types of neurons and glial cells during CNS development...
  77. ncbi request reprint Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells
    Takahito Yoshizaki
    Department of Physiuology, Keio University School of Medicine, Tokyo, Japan
    Neurosci Lett 363:33-7. 2004
    ..This strategy of isolation and transplantation of ES-cell-derived DA neurons should be useful for cellular and molecular studies of DA neurons and for clinical application in the treatment of Parkinson's disease...
  78. pmc Cardiac neural crest cells contribute to the dormant multipotent stem cell in the mammalian heart
    Yuichi Tomita
    Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo, Japan
    J Cell Biol 170:1135-46. 2005
    ....
  79. pmc Drosophila caspase transduces Shaggy/GSK-3beta kinase activity in neural precursor development
    Hirotaka Kanuka
    Department of Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo ku, Tokyo, Japan
    EMBO J 24:3793-806. 2005
    ..These results imply a novel role for caspases in modulating cell signaling pathways through substrate cleavage in neural precursor development...
  80. pmc A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells
    Masanori Sakaguchi
    Department of Physiology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Proc Natl Acad Sci U S A 103:7112-7. 2006
    ..Based on results from intraventricular infusion experiments and phenotypic analyses of knockout mice, we demonstrate that Galectin-1 is an endogenous factor that promotes the proliferation of NSCs in the adult brain...
  81. ncbi request reprint Augmented therapeutic efficacy of an oncolytic herpes simplex virus type 1 mutant expressing ICP34.5 under the transcriptional control of musashi1 promoter in the treatment of malignant glioma
    Ryuichi Kanai
    Molecular Neurosurgery Laboratory, Department of Neurosurgery, School of Medicine, Keio University, Tokyo 160 8582, Japan
    Hum Gene Ther 18:63-73. 2007
    ..These results suggest that oncolytic HSV-1 expressing ICP34.5 under the transcriptional control of the musashi1 gene promoter could be a promising therapeutic agent for the treatment of malignant glioma...
  82. pmc The neural stem/progenitor cell marker nestin is expressed in proliferative endothelial cells, but not in mature vasculature
    Sayuri Suzuki
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Histochem Cytochem 58:721-30. 2010
    ..Immunohistochemistry showed nestin expression in neovascular cells from two distinct murine models. Our results demonstrate that nestin can be used as a marker protein for neovascularization...
  83. pmc Interleukin-6 receptor-mediated activation of signal transducer and activator of transcription-3 (STAT3) promotes choroidal neovascularization
    Kanako Izumi-Nagai
    Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Am J Pathol 170:2149-58. 2007
    ....
  84. doi request reprint Transplantation of dendritic cells promotes functional recovery from spinal cord injury in common marmoset
    Masae Yaguchi
    Neuroimmunology Research Group, Keio University School of Medicine, Tokyo 160 8582, Japan
    Neurosci Res 65:384-92. 2009
    ..These results suggest DC therapy to possibly be beneficial in primates with SCI and that this treatment has potential for clinical application...
  85. doi request reprint Spatiotemporal recapitulation of central nervous system development by murine embryonic stem cell-derived neural stem/progenitor cells
    Yohei Okada
    Department of Physiology, Keio University, School of Medicine, Shinjuku ku, Tokyo, Japan
    Stem Cells 26:3086-98. 2008
    ....
  86. doi request reprint Induction of neural crest cells from mouse embryonic stem cells in a serum-free monolayer culture
    Yuko Aihara
    Department of Life Sciences Biology, Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan
    Int J Dev Biol 54:1287-94. 2010
    ..P0-6 cells cultured using this protocol expressed EGFP. This protocol could be used to help clarify the mechanisms by which cells differentiate into the NC lineage and to assist the development of applications for clinical therapy...
  87. ncbi request reprint Differential expression of Pax6 and Ngn2 between pair-generated cortical neurons
    Ayano Kawaguchi
    Laboratory for Cell Culture Development, Brain Science Institute, RIKEN, Wako, Saitama, Japan
    J Neurosci Res 78:784-95. 2004
    ..We suggest that pair progenitor cells contribute to the generation of neuronal diversity through cell-intrinsic heterogeneity and asymmetric division...
  88. pmc The dual origin of the peripheral olfactory system: placode and neural crest
    Hiroyuki Katoh
    Department of Orthopaedic Surgery, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    Mol Brain 4:34. 2011
    ....
  89. pmc Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies
    Shigeki Ohta
    Neuroimmunology Research Group, Keio University School of Medicine, Tokyo, Japan
    Immunology 123:566-74. 2008
    ..These results suggest that BM-derived DCs from the common marmoset may be useful for biological analysis and for preclinical studies on cell therapy for central nervous system diseases and cancer...
  90. pmc Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-signaling cytokines in rodents
    Hideaki Kanazawa
    Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    J Clin Invest 120:408-21. 2010
    ..Thus, CHF causes target-dependent cholinergic transdifferentiation of the cardiac SNS via gp130-signaling cytokines secreted from the failing myocardium...
  91. pmc Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells
    Satoshi Kawase
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, Japan
    Mol Brain 4:14. 2011
    ..Based on previous findings, Msi1 is likely to be a key regulator for maintaining the characteristics of self-renewing stem cells. However, the mechanisms regulating Msi1 expression are not yet clear...
  92. doi request reprint Comparative study of methods for administering neural stem/progenitor cells to treat spinal cord injury in mice
    Yuichiro Takahashi
    Department of Orthopaedic Surgery, School of Medicine, Keio University, Tokyo, Japan
    Cell Transplant 20:727-39. 2011
    ..In terms of grafted cell survival and safety, we conclude that the IL application of NS/PCs is the most effective and feasible method for transplanting NS/PCs into the SCI site...
  93. pmc Isolation and function of mouse tissue resident vascular precursors marked by myelin protein zero
    Yoshiaki Kubota
    Department of Cell Differentiation, The Sakaguchi Laboratory, School of Medicine, Keio University, Shinjuku ku, Tokyo 160 8582, Japan
    J Exp Med 208:949-60. 2011
    ..Thus, in postnatal mice, although growing vessels are formed primarily by angiogenesis from preexisting vessels, a minor population of arterial endothelia may be derived from tissue-resident vascular precursor cells...
  94. pmc Sox10-Venus mice: a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes
    Shinsuke Shibata
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Brain 3:31. 2010
    ..We generated Sox10-Venus BAC transgenic mice to monitor Sox10 expression in both normal development and in pathological processes...
  95. doi request reprint Gene targeting and subsequent site-specific transgenesis at the β-actin (ACTB) locus in common marmoset embryonic stem cells
    Seiji Shiozawa
    Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    Stem Cells Dev 20:1587-99. 2011
    ..Moreover, the creation of a transgene acceptor locus for site-specific transgenesis will be a powerful tool, similar to the ROSA26 locus in mice...
  96. doi request reprint Regulation of adult neural progenitor cells by Galectin-1/beta1 Integrin interaction
    Masanori Sakaguchi
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurochem 113:1516-24. 2010
    ..These results suggest that the interaction between beta1 Integrin and Galectin-1 plays an important role in regulating the number of adult NPCs through mechanisms including cell adhesion...
  97. pmc Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP
    Hironori Kawahara
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo 160 8582, Japan
    J Cell Biol 181:639-53. 2008
    ..These results provide insight into the mechanism by which sequence-specific translational repression occurs in stem cells through the control of translation initiation...
  98. ncbi request reprint Two distinct stem cell lineages in murine bone marrow
    Yoko Koide
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Stem Cells 25:1213-21. 2007
    ..The results may reinforce the urgent need for a more comprehensive view of the mesenchymal stem cell identity and characteristics. Disclosure of potential conflicts of interest is found at the end of this article...
  99. ncbi request reprint Beta-catenin signaling promotes proliferation of progenitor cells in the adult mouse subventricular zone
    Kazuhide Adachi
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Stem Cells 25:2827-36. 2007
    ..These results suggest that beta-catenin signaling plays a role in the proliferation of progenitor cells in the SVZ of the adult mouse brain...
  100. ncbi request reprint Mapping spatio-temporal activation of Notch signaling during neurogenesis and gliogenesis in the developing mouse brain
    Akinori Tokunaga
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurochem 90:142-54. 2004
    ..Taken together, the present method has enabled us to determine the timing, gradients, and boundaries of the activation of Notch signaling...
  101. ncbi request reprint Targeted expression of anti-apoptotic protein p35 in oligodendrocytes reduces delayed demyelination and functional impairment after spinal cord injury
    Mutsuhiro Tamura
    Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
    Glia 51:312-21. 2005
    ..Thus, the inhibition of OLG apoptosis decreased the extent of functional impairment after SCI. These findings suggest that the inhibition of OLG apoptosis may be a potential treatment for SCI...