H Okano

Summary

Affiliation: Keio University
Country: Japan

Publications

  1. ncbi request reprint Chondroitinase ABC combined with neural stem/progenitor cell transplantation enhances graft cell migration and outgrowth of growth-associated protein-43-positive fibers after rat spinal cord injury
    Takeshi Ikegami
    Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
    Eur J Neurosci 22:3036-46. 2005
  2. ncbi request reprint [Transplantation of neural stem cells for spinal cord injury]
    Masaya Nakamura
    Department of Orthopedic Surgery, Keio University, School of Medicine
    Rinsho Shinkeigaku 45:874-6. 2005
  3. pmc Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP
    Hironori Kawahara
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo 160 8582, Japan
    J Cell Biol 181:639-53. 2008
  4. pmc Roles of ES cell-derived gliogenic neural stem/progenitor cells in functional recovery after spinal cord injury
    Gentaro Kumagai
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 4:e7706. 2009
  5. pmc Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation
    Yoko Ozawa
    Department of Ophthalmology, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Biol Chem 283:24561-70. 2008
  6. pmc Generation of human melanocytes from induced pluripotent stem cells
    Shigeki Ohta
    Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 6:e16182. 2011
  7. pmc Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells
    Masanao Tabuse
    Neuroimmunology Research Group, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Cancer 10:60. 2011
  8. pmc Musashi1 regulates breast tumor cell proliferation and is a prognostic indicator of poor survival
    Xiao Yang Wang
    Department of Oncology, Georgetown University, and Lombardi Comprehensive Cancer Center, Washington, DC 20007, USA
    Mol Cancer 9:221. 2010
  9. pmc Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells
    Satoshi Kawase
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, Japan
    Mol Brain 4:14. 2011
  10. pmc Galectin-1 is expressed in early-type neural progenitor cells and down-regulates neurogenesis in the adult hippocampus
    Yoichi Imaizumi
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Mol Brain 4:7. 2011

Detail Information

Publications117 found, 100 shown here

  1. ncbi request reprint Chondroitinase ABC combined with neural stem/progenitor cell transplantation enhances graft cell migration and outgrowth of growth-associated protein-43-positive fibers after rat spinal cord injury
    Takeshi Ikegami
    Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
    Eur J Neurosci 22:3036-46. 2005
    ....
  2. ncbi request reprint [Transplantation of neural stem cells for spinal cord injury]
    Masaya Nakamura
    Department of Orthopedic Surgery, Keio University, School of Medicine
    Rinsho Shinkeigaku 45:874-6. 2005
    ..These results indicate that in vitro expanded NSCs derived from human fetal spinal cord are useful sources for the therapeutics of spinal cord injury in primates...
  3. pmc Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP
    Hironori Kawahara
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo 160 8582, Japan
    J Cell Biol 181:639-53. 2008
    ..These results provide insight into the mechanism by which sequence-specific translational repression occurs in stem cells through the control of translation initiation...
  4. pmc Roles of ES cell-derived gliogenic neural stem/progenitor cells in functional recovery after spinal cord injury
    Gentaro Kumagai
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 4:e7706. 2009
    ..These findings suggest that gliogenic NS/PCs are effective for promoting the recovery from SCI, and provide essential insight into the mechanisms through which cellular transplantation leads to functional improvement after SCI...
  5. pmc Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation
    Yoko Ozawa
    Department of Ophthalmology, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Biol Chem 283:24561-70. 2008
    ..These findings suggest SOCS3 as a potential therapeutic target molecule for protecting photoreceptor cell function during inflammation...
  6. pmc Generation of human melanocytes from induced pluripotent stem cells
    Shigeki Ohta
    Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan
    PLoS ONE 6:e16182. 2011
    ..This in vitro differentiation system should prove useful for understanding human melanocyte biology and revealing the mechanism of various pigment cell disorders, including melanoma...
  7. pmc Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells
    Masanao Tabuse
    Neuroimmunology Research Group, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Cancer 10:60. 2011
    ..HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. They also behave as oncogenes in some malignancies...
  8. pmc Musashi1 regulates breast tumor cell proliferation and is a prognostic indicator of poor survival
    Xiao Yang Wang
    Department of Oncology, Georgetown University, and Lombardi Comprehensive Cancer Center, Washington, DC 20007, USA
    Mol Cancer 9:221. 2010
    ..It is unknown how Msi1 relates to other breast cancer markers, whether it denotes tumor initiating cells (TICs), and how it affects gene expression and tumor cell survival in breast cancer cells...
  9. pmc Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells
    Satoshi Kawase
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, Japan
    Mol Brain 4:14. 2011
    ..Based on previous findings, Msi1 is likely to be a key regulator for maintaining the characteristics of self-renewing stem cells. However, the mechanisms regulating Msi1 expression are not yet clear...
  10. pmc Galectin-1 is expressed in early-type neural progenitor cells and down-regulates neurogenesis in the adult hippocampus
    Yoichi Imaizumi
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Mol Brain 4:7. 2011
    ..However, little is known about its detailed expression profile in the NPCs and functions in adult neurogenesis in the DG...
  11. pmc Sox10-Venus mice: a new tool for real-time labeling of neural crest lineage cells and oligodendrocytes
    Shinsuke Shibata
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Brain 3:31. 2010
    ..We generated Sox10-Venus BAC transgenic mice to monitor Sox10 expression in both normal development and in pathological processes...
  12. pmc "Color Timer" mice: visualization of neuronal differentiation with fluorescent proteins
    Hiroaki Kanki
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Mol Brain 3:5. 2010
    ..This two-color initial version of the "Color Timer" mouse system will provide a powerful new tool for neurogenesis research...
  13. ncbi request reprint [RNA regulation in nervous system development and differentiation]
    Hideyuki Okano
    Tanpakushitsu Kakusan Koso 48:430-7. 2003
  14. ncbi request reprint Function of RNA-binding protein Musashi-1 in stem cells
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Exp Cell Res 306:349-56. 2005
    ..In addition to its functions in NSCs, the role of mammalian Musashi-1 protein in epithelial stem cells, including intestinal and mammary gland stem cells, is attracting increasing interest...
  15. ncbi request reprint Regeneration-based therapies for spinal cord injuries
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Neurochem Int 51:68-73. 2007
    ....
  16. ncbi request reprint Strategies toward CNS-regeneration using induced pluripotent stem cells
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 112 0012, Japan
    Genome Inform 23:217-20. 2009
    ..Surprisingly, SNSs derived from c-Myc minus iPS cells generated without drug selection showed robust tumorigenesis, in spite of their potential to contribute adult chimeric mice without tumor formation...
  17. doi request reprint Cell types to order: temporal specification of CNS stem cells
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 112 0012, Japan
    Curr Opin Neurobiol 19:112-9. 2009
    ..Here we review recent findings on the molecular mechanisms of epigenetic and transcription factor regulation, which are involved in temporal specification of CNS stem cells...
  18. pmc Neural stem cells: involvement in adult neurogenesis and CNS repair
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Philos Trans R Soc Lond B Biol Sci 363:2111-22. 2008
    ....
  19. ncbi request reprint Musashi: a translational regulator of cell fate
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Cell Sci 115:1355-9. 2002
    ..In mammals it might play an important role in activating Notch signalling by repressing translation of the Notch inhibitor m-Numb...
  20. pmc Neural stem cells and strategies for the regeneration of the central nervous system
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, Shinanomachi, Shinjuku ku, Tokyo, Japan
    Proc Jpn Acad Ser B Phys Biol Sci 86:438-50. 2010
    ....
  21. ncbi request reprint Regeneration of the central nervous system using endogenous repair mechanisms
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, Shinanomachi, Shinjuku ku, Tokyo, Japan
    J Neurochem 102:1459-65. 2007
    ..In this review, we will summarize the recent advances in regeneration-based therapeutic approaches using endogenous NSCs, including the results of our own collaborative groups...
  22. ncbi request reprint Neural stem cells: progression of basic research and perspective for clinical application
    Hideyuki Okano
    Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    Keio J Med 51:115-28. 2002
    ..In this review, recent findings on the stem cell biology of the CNS and strategies for its potential therapeutic application will be discussed...
  23. ncbi request reprint Neural stem cells and regeneration of injured spinal cord
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Kidney Int 68:1927-31. 2005
    ....
  24. ncbi request reprint Isolation and transplantation of dopaminergic neurons and neural stem cells
    Hideyuki Okano
    Department of Physiology, School of Medicine, Keio University, Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Parkinsonism Relat Disord 9:23-8. 2002
    ..Transplantation of the sorted cells resulted in recovery of a rat model of the PD. This strategy should be useful for developing new therapies for PD...
  25. ncbi request reprint Transplantation of in vitro-expanded fetal neural progenitor cells results in neurogenesis and functional recovery after spinal cord contusion injury in adult rats
    Y Ogawa
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurosci Res 69:925-33. 2002
    ..These results indicate that in vitro-expanded neurosphere cells derived from the fetal spinal cord are a potential source for transplantable material for treatment of spinal cord injury...
  26. ncbi request reprint Stem cell biology of the central nervous system
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurosci Res 69:698-707. 2002
    ....
  27. ncbi request reprint Transplantation of neural stem cells into the spinal cord after injury
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160 8582, Japan
    Semin Cell Dev Biol 14:191-8. 2003
    ..On the other hand, in the chronic stage after injury, glial scars form in the injured site that inhibit the regeneration of neuronal axons. Thus, we believe that the optimal timing of transplantation is 1-2 weeks after injury...
  28. ncbi request reprint Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery
    Yuji Mikami
    Neuroimmunology Research Group, Keio University School of Medicine, Tokyo, Japan
    J Neurosci Res 76:453-65. 2004
    ..Our results suggest that DC-implantation exerts trophic effects, including activation of endogenous NSPCs, leading to repair of the injured adult spinal cord...
  29. pmc The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA
    T Imai
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo 160 8582
    Mol Cell Biol 21:3888-900. 2001
    ..Furthermore, we found that Notch signaling activity was increased by Msi1 expression in connection with the posttranscriptional down-regulation of the m-numb gene...
  30. doi request reprint Environmental cue-dependent dopaminergic neuronal differentiation and functional effect of grafted neuroepithelial stem cells in parkinsonian brain
    Yutaka Mine
    Department of Neurosurgery, School of Medicine, Keio University, Tokyo, Japan
    Neurosurgery 65:741-53; discussion 753. 2009
    ....
  31. ncbi request reprint Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells
    Takahito Yoshizaki
    Department of Physiuology, Keio University School of Medicine, Tokyo, Japan
    Neurosci Lett 363:33-7. 2004
    ..This strategy of isolation and transplantation of ES-cell-derived DA neurons should be useful for cellular and molecular studies of DA neurons and for clinical application in the treatment of Parkinson's disease...
  32. ncbi request reprint Mapping spatio-temporal activation of Notch signaling during neurogenesis and gliogenesis in the developing mouse brain
    Akinori Tokunaga
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurochem 90:142-54. 2004
    ..Taken together, the present method has enabled us to determine the timing, gradients, and boundaries of the activation of Notch signaling...
  33. ncbi request reprint [Neural stem cells: their identification, isolation and potential therapeutic application]
    Hideyuki Okano
    Department of Physiology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582
    Seikagaku 74:17-26. 2002
  34. ncbi request reprint Blockade of interleukin-6 receptor suppresses reactive astrogliosis and ameliorates functional recovery in experimental spinal cord injury
    S Okada
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    J Neurosci Res 76:265-76. 2004
    ..These findings suggest that neutralization of IL-6 signaling in the acute phase of SCI represents an attractive option for the treatment of SCI...
  35. ncbi request reprint Establishment of graded spinal cord injury model in a nonhuman primate: the common marmoset
    A Iwanami
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    J Neurosci Res 80:172-81. 2005
    ....
  36. ncbi request reprint Comparison between fetal spinal-cord- and forebrain-derived neural stem/progenitor cells as a source of transplantation for spinal cord injury
    Kota Watanabe
    Department of Orthopaedic Surgery, Keio University, Tokyo, Japan
    Dev Neurosci 26:275-87. 2004
    ..In conclusion, forebrain-derived NSPCs could be used as an alternative to spinal-cord-derived NSPCs as a potential therapeutic agent for spinal cord injuries...
  37. ncbi request reprint Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum
    Toru Yamashita
    Bridgestone Laboratory of Developmental and Regenerative Neurobiology, Keio University School of Medicine, Shinanomachi, Tokyo 160 8582, Japan
    J Neurosci 26:6627-36. 2006
    ..These results highlight the role of the SVZ in neuronal regeneration after a stroke and its potential as an important therapeutic target for various neurological disorders...
  38. ncbi request reprint In vivo imaging of engrafted neural stem cells: its application in evaluating the optimal timing of transplantation for spinal cord injury
    Seiji Okada
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    FASEB J 19:1839-41. 2005
    ..Optimization of cell therapies can be greatly accelerated and refined by imaging, and the methods in the present study can be widely applied to various research fields of regeneration medicine, including transplantation study...
  39. pmc Therapeutic potential of appropriately evaluated safe-induced pluripotent stem cells for spinal cord injury
    Osahiko Tsuji
    Department of Physiology, School of Medicine, Keio University, Shinjuku, Tokyo 160 8582, Japan
    Proc Natl Acad Sci U S A 107:12704-9. 2010
    ..These findings suggest that pre-evaluated safe iPS clone-derived neural stem/progenitor cells may be a promising cell source for transplantation therapy for SCI...
  40. doi request reprint Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development
    Hayato Naka
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Nat Neurosci 11:1014-23. 2008
    ..These findings reveal a part of the timer mechanisms for generating diverse types of neurons and glial cells during CNS development...
  41. doi request reprint Transplantation of galectin-1-expressing human neural stem cells into the injured spinal cord of adult common marmosets
    Junichi Yamane
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    J Neurosci Res 88:1394-405. 2010
    ..These findings suggest that Gal-NS/PCs have better therapeutic potential than NS/PCs for SCI in nonhuman primates and that human Gal-NS/PC transplantation might be a feasible treatment for human SCI...
  42. ncbi request reprint Epidermal growth factor signaling mediated by grb2 associated binder1 is required for the spatiotemporally regulated proliferation of olig2-expressing progenitors in the embryonic spinal cord
    Yoshika Hayakawa-Yano
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Stem Cells 25:1410-22. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  43. ncbi request reprint Distinct functions of human numb isoforms revealed by misexpression in the neural stem cell lineage in the Drosophila larval brain
    Masako Toriya
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Dev Neurosci 28:142-55. 2006
    ....
  44. ncbi request reprint A selective Sema3A inhibitor enhances regenerative responses and functional recovery of the injured spinal cord
    Shinjiro Kaneko
    Department of Orthopaedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku ku, Tokyo 160 8582, Japan
    Nat Med 12:1380-9. 2006
    ..Thus, Sema3A is essential for the inhibition of axonal regeneration and other regenerative responses after spinal cord injury (SCI). These results support the possibility of using Sema3A inhibitors in the treatment of human SCI...
  45. ncbi request reprint Transplantation of human neural stem cells for spinal cord injury in primates
    A Iwanami
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    J Neurosci Res 80:182-90. 2005
    ..These findings show that NSPC transplantation was effective for SCI in primates and suggest that human NSPC transplantation could be a feasible treatment for human SCI...
  46. ncbi request reprint [Regulatory mechanisms of neural stem cell and strategies for therapy]
    Seiji Okada
    Department of Physiology, Keio University School of Medicine
    Nihon Rinsho 61:449-56. 2003
    ..In this article, we review some of the basic notions regarding the extracellular factors and signal transduction cascades involved in the differentiation and maintenance of NSCs...
  47. ncbi request reprint Flow cytometric analysis of neural stem cells in the developing and adult mouse brain
    Ayako Murayama
    Department of Physiology, Keio University School of Medicine, Shinanomachi, Shinjuku ku, Tokyo, Japan
    J Neurosci Res 69:837-47. 2002
    ..FACS analysis showed that SP cells from adults were included to some extent in the CD24(low)/PNA(low) fraction, where NSCs were greatly concentrated. Collectively, the characteristics of NSCs were not uniform and changed developmentally...
  48. doi request reprint Drosophila big brain does not act as a water channel, but mediates cell adhesion
    Kimiko Tatsumi
    Department of Pharmacology, School of Medicine, Keio University, Tokyo, Japan
    FEBS Lett 583:2077-82. 2009
    ..Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important for Drosophila neurogenesis...
  49. ncbi request reprint Involvement of Hu and heterogeneous nuclear ribonucleoprotein K in neuronal differentiation through p21 mRNA post-transcriptional regulation
    Masato Yano
    CREST JST and the Department of Physiology, Keio University School of Medicine, Tokyo 160 8582, Japan
    J Biol Chem 280:12690-9. 2005
    ....
  50. ncbi request reprint Establishment of three-dimensional culture of neural stem/progenitor cells in collagen Type-1 Gel
    Kota Watanabe
    Department of Orthopaedic Surgery, Keio University 35 Shinanomachi, Shinjuku, Tokyo 160 8582, Japan
    Restor Neurol Neurosci 25:109-17. 2007
    ..We, then, evaluated the ability of NSPCs to differentiate under the optimal condition...
  51. ncbi request reprint [Neural stem cells: the basic biology and prospects for brain repair]
    H Okano
    Department of Physiology, Keio University School of Medicine
    Rinsho Shinkeigaku 41:1036-40. 2001
    ....
  52. ncbi request reprint Downregulation of STAT3 activation is required for presumptive rod photoreceptor cells to differentiate in the postnatal retina
    Yoko Ozawa
    Department of Physiology, Keio University School of Medicine, Shinjuku, Tokyo, Japan
    Mol Cell Neurosci 26:258-70. 2004
    ..Thus, we conclude that downregulation of STAT3 activation is required, but insufficient, for rod photoreceptor cell differentiation in the postnatal retina...
  53. pmc The neural stem/progenitor cell marker nestin is expressed in proliferative endothelial cells, but not in mature vasculature
    Sayuri Suzuki
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Histochem Cytochem 58:721-30. 2010
    ..Immunohistochemistry showed nestin expression in neovascular cells from two distinct murine models. Our results demonstrate that nestin can be used as a marker protein for neovascularization...
  54. ncbi request reprint [Regeneration of central nervous system: its concept and strategy]
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine
    Rinsho Shinkeigaku 43:824-6. 2003
    ..In this review, we would like to take Parkinson's disease and spinal cord injury as examples to discuss actual strategy aiming for CNS-regeneration...
  55. ncbi request reprint Human neural stem/progenitor cells, expanded in long-term neurosphere culture, promote functional recovery after focal ischemia in Mongolian gerbils
    Satoru Ishibashi
    Department of Neurology and Neurological Science, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan
    J Neurosci Res 78:215-23. 2004
    ..These results indicate that in vitro-expanded human neurosphere cells are a potential source for transplantable material for treatment of stroke...
  56. ncbi request reprint Expression of a candidate marker for progenitor cells, Musashi-1, in the proliferative regions of human antrum and its decreased expression in intestinal metaplasia
    Y Akasaka
    Department of Pathology, School of Medicine, Toho University, Tokyo, Japan
    Histopathology 47:348-56. 2005
    ..The aim of the present study was to clarify whether Musashi-1 (Msi-1), which has recently been proposed as a stem cell marker in mouse intestine, serves as a marker for progenitor cells in human stomach...
  57. ncbi request reprint Enhanced therapeutic efficacy of G207 for the treatment of glioma through Musashi1 promoter retargeting of gamma34.5-mediated virulence
    R Kanai
    Molecular Neurosurgery Laboratory, School of Medicine, Keio University, Tokyo, Japan
    Gene Ther 13:106-16. 2006
    ..These results suggest that transcriptional regulation of ICP34.5 by P/musashi1 can be used to target HSV-1 virulence toward gliomas while maintaining the desirable neuroattenuated phenotype...
  58. ncbi request reprint tincar encodes a novel transmembrane protein expressed in the Tinman-expressing cardioblasts of Drosophila
    Yuki Hirota
    Department of Physiology, Keio University School of Medicine, Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Gene Expr Patterns 2:323-7. 2002
    ..In the non-Tin-expressing pairs of cardioblasts, tinc transcription seemed to be repressed by Seven-up...
  59. pmc The RNA-binding protein HuD regulates neuronal cell identity and maturation
    Wado Akamatsu
    Department of Physiology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Proc Natl Acad Sci U S A 102:4625-30. 2005
    ..Taken together, the results suggest that HuD is required at multiple points during neuronal development, including negative regulation of proliferative activity and neuronal cell-fate acquisition of neural stem/progenitor cells...
  60. ncbi request reprint Sox21 is a repressor of neuronal differentiation and is antagonized by YB-1
    Hiroyuki Ohba
    CREST JST and Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Neurosci Lett 358:157-60. 2004
    ..These results suggest that Sox21 is a repressor of neuronal differentiation in the developing nervous system...
  61. doi request reprint Evaluation of human fetal neural stem/progenitor cells as a source for cell replacement therapy for neurological disorders: properties and tumorigenicity after long-term in vitro maintenance
    Daisuke Ogawa
    Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
    J Neurosci Res 87:307-17. 2009
    ..These findings support the idea that hNS/PCs may represent a nontumorigenic, safe, and appropriate cell source for regenerative therapies for neurological disorders...
  62. pmc Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow
    Satoru Morikawa
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    J Exp Med 206:2483-96. 2009
    ..Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities...
  63. ncbi request reprint Growth and differentiation potential of main- and side-population cells derived from murine skeletal muscle
    Tetsuro Tamaki
    Department of Physiology, Division of Human Structure and Function, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259 1193, Japan
    Exp Cell Res 291:83-90. 2003
    ..These results indicate that the MP cell population is about 99.9% responsible for the reported in vitro myogenic-endothelial responses of skeletal muscle-derived cells...
  64. ncbi request reprint [Regeneration of the central nervous system using iPS cell-technologies]
    Hideyuki Okano
    Department of Physiology, Keio University School of Medicine
    Rinsho Shinkeigaku 49:825-6. 2009
    ..As a result, we found that properly pre-evaluated iPS clone-derived neural stem/progenitor cells may be a promising cell source for future transplantation therapy of SCI...
  65. doi request reprint Musashi1 as a marker of reactive astrocytes after transient focal brain ischemia
    Koichi Oki
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Neurosci Res 66:390-5. 2010
    ..However, the difference in the distributions of Msi1 and Nestin suggests that some of their features may differ in reactive astrocytes...
  66. pmc Cardiac neural crest cells contribute to the dormant multipotent stem cell in the mammalian heart
    Yuichi Tomita
    Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, Tokyo, Japan
    J Cell Biol 170:1135-46. 2005
    ....
  67. ncbi request reprint Blockade of interleukin-6 signaling aggravates ischemic cerebral damage in mice: possible involvement of Stat3 activation in the protection of neurons
    Toru Yamashita
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurochem 94:459-68. 2005
    ..These results suggest that endogenous IL-6 plays a critical role in preventing damaged neurons from undergoing apoptosis in the acute phase of cerebral ischemia and that its role may be mediated by Stat3 activation...
  68. ncbi request reprint Unexpectedly efficient homing capacity of purified murine hematopoietic stem cells
    Yumi Matsuzaki
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, 160 8582, Tokyo, Japan
    Immunity 20:87-93. 2004
    ..This led us to propose high marrow-seeding efficiency as a specific characteristic of primitive HSCs, in addition to their self-renewal and multipotent capacity...
  69. doi request reprint Regulation of adult neural progenitor cells by Galectin-1/beta1 Integrin interaction
    Masanori Sakaguchi
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    J Neurochem 113:1516-24. 2010
    ..These results suggest that the interaction between beta1 Integrin and Galectin-1 plays an important role in regulating the number of adult NPCs through mechanisms including cell adhesion...
  70. ncbi request reprint Musashi-1 expression in postnatal mouse olfactory epithelium
    Kenta Watanabe
    Department of Otolaryngology, Faculty of Medicine, University of Tokyo, Tokyo, Japan
    Neuroreport 18:641-4. 2007
    ..These findings are consistent with the fact that globose basal cells are proliferating olfactory precursor cells and that their ability to generate new neurons decreases with aging...
  71. doi request reprint Atlas of the developing brain of the marmoset monkey constructed using magnetic resonance histology
    K Hikishima
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Neuroscience 230:102-13. 2013
    ..The multidimensional atlas and the MRI tools developed herein are anticipated to further our understanding of the developing primate brain...
  72. ncbi request reprint Brain from bone: efficient "meta-differentiation" of marrow stroma-derived mature osteoblasts to neurons with Noggin or a demethylating agent
    J Kohyama
    Department of Pathology, Keio University School of Medicine, Tokyo, Japan
    Differentiation 68:235-44. 2001
    ..Marrow stroma is therefore a potential source of cells for neural cell transplantation...
  73. ncbi request reprint Role of IL-6 in spinal cord injury in a mouse model
    Masaya Nakamura
    Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo 160 8582, Japan
    Clin Rev Allergy Immunol 28:197-204. 2005
    ..This article presents the data from this investigation and discusses the relationship between IL-6 signals and spinal cord injury...
  74. ncbi request reprint New neurons follow the flow of cerebrospinal fluid in the adult brain
    Kazunobu Sawamoto
    Department of Neurological Surgery and Developmental and Stem Cell Biology Program, University of California San Francisco, San Francisco, CA 94143, USA
    Science 311:629-32. 2006
    ..Results suggest that polarized epithelial cells contribute important vectorial information for guidance of young, migrating neurons...
  75. ncbi request reprint Two distinct stem cell lineages in murine bone marrow
    Yoko Koide
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Stem Cells 25:1213-21. 2007
    ..The results may reinforce the urgent need for a more comprehensive view of the mesenchymal stem cell identity and characteristics. Disclosure of potential conflicts of interest is found at the end of this article...
  76. doi request reprint Spatiotemporal recapitulation of central nervous system development by murine embryonic stem cell-derived neural stem/progenitor cells
    Yohei Okada
    Department of Physiology, Keio University, School of Medicine, Shinjuku ku, Tokyo, Japan
    Stem Cells 26:3086-98. 2008
    ....
  77. ncbi request reprint Potential functional neural repair with grafted neural stem cells of early embryonic neuroepithelial origin
    Koichi Uchida
    Department of Neurosurgery, School of Medicine, Keio University, Tokyo 160 8582, Japan
    Neurosci Res 52:276-86. 2005
    ..Neuroepithelial stem cells are therefore an attractive candidate as a source of donor material for intracerebral grafting in functional repair...
  78. ncbi request reprint Visualization of spatiotemporal activation of Notch signaling: live monitoring and significance in neural development
    Jun Kohyama
    Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Dev Biol 286:311-25. 2005
    ....
  79. ncbi request reprint Grafted swine neuroepithelial stem cells can form myelinated axons and both efferent and afferent synapses with xenogeneic rat neurons
    Koichi Uchida
    Department of Neurosurgery, School of Medicine, Keio University, Shinjuku, Tokyo, Japan
    J Neurosci Res 72:661-9. 2003
    ....
  80. ncbi request reprint Expression of Musashi-1 in the rat stomach and changes during mucosal injury and restitution
    Hiroshi Nagata
    Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    FEBS Lett 580:27-33. 2006
    ..We conclude that a subpopulation of parietal cells acts as a source of Musashi-1, which contributes to rapid re-epithelization by restoration of stem cells and regulation of cell differentiation...
  81. pmc Effect of κ-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells
    N Kuzumaki
    Department of Physiology, Keio University, School of Medicine, 35 Shinanomachi Shinjuku ku, Tokyo 160 8582, Japan
    Br J Cancer 106:1148-52. 2012
    ..It is becoming increasingly recognised that opioids are responsible for tumour growth. However, the effects of opioids on tumour growth have been controversial...
  82. doi request reprint Population-averaged standard template brain atlas for the common marmoset (Callithrix jacchus)
    K Hikishima
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Neuroimage 54:2741-9. 2011
    ..2 mm)(3) at 7-Tesla and DARTEL algorithm in SPM8. Whole brain templates are available at International Neuroinformatics Japan Node website, http://brainatlas.brain.riken.jp/marmoset/...
  83. pmc Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production
    H Fukuda
    Department of Pathology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo 160 0016, Japan
    Br J Cancer 105:1615-24. 2011
    ..Although matrix metalloproteinases (MMPs) are implicated in tumourigenesis and cancer progression, the role of MMP-13 in melanoma cell metastases is poorly understood...
  84. ncbi request reprint Expression and function of galectin-1 in adult neural stem cells
    M Sakaguchi
    Department of Physiology, Keio University School of Medicine, Shinanomachi, Tokyo, Japan
    Cell Mol Life Sci 64:1254-8. 2007
    ..This evidence raises the possibility that glycans play roles in the proliferation of adult NSCs...
  85. ncbi request reprint In vivo imaging in humanized mice
    H Masuda
    Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Curr Top Microbiol Immunol 324:179-96. 2008
    ..Finally, we introduce a humanized mouse and a novel in vivo imaging system that we have developed. We also discuss real-time observations of reconstructs and clinical manifestations...
  86. ncbi request reprint Expression of the neural RNA-binding protein Musashi1 in human gliomas
    M Toda
    Department of Physiology, Keio University School of Medicine, Tokyo, Japan
    Glia 34:1-7. 2001
    ..These results suggest that primary CNS tumors may share gene expression patterns with primitive, undifferentiated CNS cells and that Musashi1 may be a useful marker for the diagnosis of CNS tumors...
  87. ncbi request reprint Anti-pressor effect of a Chinese-Japanese herbal medicine, saiko-ka-ryukotsu-borei-to on hemodynamics in rabbits
    H Okano
    Department of Physiological Hygiene, National Institute of Public Health, Tokyo, Japan
    In Vivo 13:333-7. 1999
    ..These results suggest that the anti-pressor effect of TJ-12 might be apparently attributable to the inhibition of NA-induced vasoconstriction...
  88. ncbi request reprint Implication of cyclooxygenase-2 on enhanced proliferation of neural progenitor cells in the adult mouse hippocampus after ischemia
    Tsutomu Sasaki
    Division of Strokology, Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Osaka, Japan
    J Neurosci Res 72:461-71. 2003
    ..These results demonstrate that COX-2 is an important modulator in enhancement of proliferation of neural progenitor cells after ischemia...
  89. pmc Musculin/MyoR is expressed in kidney side population cells and can regulate their function
    Keiichi Hishikawa
    Department of Clinical Renal Regeneration, Graduate School of Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 8655, Japan
    J Cell Biol 169:921-8. 2005
    ..Our results suggest that musculin/MyoR may play important roles not only in developmental processes but also in regenerative processes in adult tissue...
  90. ncbi request reprint Enhanced proliferation of progenitor cells in the subventricular zone and limited neuronal production in the striatum and neocortex of adult macaque monkeys after global cerebral ischemia
    Anton B Tonchev
    Department of Restorative Neurosurgery, Division of Neuroscience, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Japan
    J Neurosci Res 81:776-88. 2005
    ..Taken together, our data suggest an enhanced neurogenic response in the adult primate telencephalon after a cerebral ischemic insult...
  91. ncbi request reprint Differential expression of Musashi1 and nestin in the adult rat hippocampus after ischemia
    Yoshiki Yagita
    Division of Strokology, Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita City, Osaka, Japan
    J Neurosci Res 69:750-6. 2002
    ..In contrast, very few nestin-positive cells were labeled by BrdU. Our results suggest that neuronal progenitor cells in the SGZ expressed Msi1 but not nestin...
  92. ncbi request reprint Drosophila homeodomain protein REPO controls glial differentiation by cooperating with ETS and BTB transcription factors
    Yoshihiro Yuasa
    Division of Neuroanatomy D12, Department of Neuroscience, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    Development 130:2419-28. 2003
    ..In addition to promoting glial differentiation, we found that REPO is also necessary to suppress neuronal development, cooperating with TTK69. We propose that REPO plays a key role in both glial development and diversification...
  93. ncbi request reprint Differential proliferative response in the postischemic hippocampus, temporal cortex, and olfactory bulb of young adult macaque monkeys
    Anton B Tonchev
    Department of Neurosurgery, Kanazawa University Graduate School of Medical Science, Kanazawa City, Japan
    Glia 42:209-24. 2003
    ..Their number and distribution were unaltered by ischemia. Our results demonstrate that cell proliferation and differentiation in the adult macaque brain and olfactory bulb are differentially affected by a common insult...
  94. ncbi request reprint Prospective characterization of neural stem cells by flow cytometry analysis using a combination of surface markers
    Masako Nagato
    Department of Pediatrics, Faculty of Medicine, Kyoto University, Kyoto, Japan
    J Neurosci Res 80:456-66. 2005
    ..These results indicate the usefulness of syndecan-1, Notch-1, and integrin-beta1 as NSC markers...
  95. ncbi request reprint Evaluation of in vitro proliferative activity of human fetal neural stem/progenitor cells using indirect measurements of viable cells based on cellular metabolic activity
    Yonehiro Kanemura
    Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, Amagasaki, Hyogo, Japan
    J Neurosci Res 69:869-79. 2002
    ..Our findings indicated that indirect measurements of viable cells based on metabolic activity, especially the ATP assay, are very effective and reproducible ways to determine the numbers of viable human NSPCs in intact neurospheres...
  96. ncbi request reprint Nestin enhancer requirements for expression in normal and injured adult CNS
    Clas B Johansson
    Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, Stockholm, Sweden
    J Neurosci Res 69:784-94. 2002
    ..Collectively, this suggests a species difference for the nestin enhancer function in adult CNS and that elements outside the second intron enhancer are required for the full injury response in vivo...
  97. ncbi request reprint Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells
    Mohammed Omedul Islam
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Hyogo 661 0974, Japan
    FEBS Lett 579:3473-80. 2005
    ..ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker...
  98. ncbi request reprint Identification of Musashi1-positive cells in human normal and neoplastic neuroepithelial tissues by immunohistochemical methods
    Yonehiro Kanemura
    Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, Osaka, Japan
    Methods Mol Biol 198:273-81. 2002
  99. ncbi request reprint Proliferation of neural and neuronal progenitors after global brain ischemia in young adult macaque monkeys
    Anton B Tonchev
    Department of Neurosurgery, Division of Neuroscience, Kanazawa University Graduate School of Medical Science, Kanazawa, 920 8641, Japan
    Mol Cell Neurosci 23:292-301. 2003
    ..Our results suggest that ischemia activates endogenous neuronal and glial precursors residing in diverse locations of the adult primate central nervous system...
  100. ncbi request reprint Bone marrow-derived cells express matrix metalloproteinases and contribute to regression of liver fibrosis in mice
    Reiichi Higashiyama
    Liver Fibrosis Research Unit, Department of Community Health, Tokai University School of Medicine, Isehara, Japan
    Hepatology 45:213-22. 2007
    ..CONCLUSION: Autologous BM cells contribute to the spontaneous regression of liver fibrosis, and their therapeutic derivation could be a new treatment strategy for intractable liver fibrosis...
  101. ncbi request reprint Components of the transcriptional Mediator complex are required for asymmetric cell division in C. elegans
    Akinori Yoda
    Division of Neuroanatomy, Osaka University Graduate School of Medicine, Kobe University, Kobe 650 0017, Japan
    Development 132:1885-93. 2005
    ..These results suggest that LET-19 and DPY-22 in the Mediator complex repress the transcription of Wnt target genes...