Research Topics
Genomes and Genes
| T NagaseSummaryAffiliation: Kazusa DNA Research Institute Country: Japan Publications
| Collaborators
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Detail Information
Publications
Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones (supplement)Daisuke Nakajima
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 9:107-15. 2002- The Kazusa cDNA project for identification of unknown human transcriptsTakahiro Nagase
Department of Human Gene Research, Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan
C R Biol 326:959-66. 2003..In the second phase of the project, which aims at bridging the human genome and proteome using the output of the first phase, we are very carefully evaluating our cDNA clones and, when necessary, experimentally revising them... - CRM1-dependent, but not ARE-mediated, nuclear export of IFN-alpha1 mRNATominori Kimura
Department of Microbiology, Kansai Medical University, Moriguchi, Osaka 570 8506, Japan
J Cell Sci 117:2259-70. 2004..However, truncation of the 3' UTR did not negatively affect the nuclear export of IFN-alpha1 mRNA that lacked the ARE, unexpectedly indicating that this CRM1-dependent mRNA export may not be mediated via the ARE... - Temporal change in mKIAA gene expression during the early stage of retinoic acid-induced neurite outgrowthKazuhide Imai
Chiba Industry Advancement Center, 2 6 Nakase, Mihama ku, Chiba 261 7126, Japan
Gene 364:114-22. 2005..These results indicate that some mKIAA genes are apparently relevant to retinoic acid-induced neurite outgrowth... - Kazusa mammalian cDNA resources: towards functional characterization of KIAA gene productsTakahiro Nagase
Department of Human Gene Research, Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan
Brief Funct Genomic Proteomic 5:4-7. 2006..In this review, we describe the current status of the Kazusa mammalian cDNA resources and the future direction of the functional characterization of KIAA genes... - Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitroT Nagase
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 7:65-73. 2000.... - Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitroT Nagase
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 6:337-45. 1999.... - Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitroT Nagase
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 7:143-50. 2000.... - Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitroT Nagase
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 7:273-81. 2000.... - Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitroT Nagase
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 8:85-95. 2001.... 
Exploration of human ORFeome: high-throughput preparation of ORF clones and efficient characterization of their protein productsTakahiro Nagase
Department of Human Genome Research, Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
DNA Res 15:137-49. 2008..Results thus obtained have demonstrated that our Flexi ORF clones are efficient for the production of HaloTag-fusion proteins that can provide a new versatile set for a variety of functional analyses of human genes...- Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteinsT Nagase
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 8:179-87. 2001.... - Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteinsT Nagase
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 8:319-27. 2001.... - Characterization of cDNA clones in size-fractionated cDNA libraries from human brainN Seki
Kazusa DNA Research Institute, Chiba, Japan
DNA Res 4:345-9. 1997.... - Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitroT Nagase
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 6:63-70. 1999.... - Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screeningM Nakayama
Kazusa DNA Research Institute, 1532 3 Yana, Kisarazu, 292 0812, Japan
Genomics 51:27-34. 1998.... - Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1T Nagase
Kazusa DNA Research Institute, Chiba, Japan
DNA Res 2:37-43. 1995..4 kb, respectively. As judged by Northern hybridization profiles, small-sized cDNAs appeared to be expressed more ubiquitously and abundantly in various tissues, compared with that of medium-sized cDNAs... - Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteinsO Ohara
Kazusa DNA Research Institute, Chiba, Japan
DNA Res 4:53-9. 1997..The set of libraries constructed here should be very useful for the accumulation of sequence data on large proteins in the human brain... - Gene identification in 1.6-Mb region of the Down syndrome region on chromosome 21M Ohira
Laboratory of Gene Structure 1, Kazusa DNA Research Institute, Chiba, Japan
Genome Res 7:47-58. 1997..These nearly full-length cDNA sequences should facilitate understanding of the detailed genome structure of the DS region and help to elucidate their role in the etiology of DS... - Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mappingM Hirosawa
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 8:1-9. 2001..In conclusion, the mapping of ESTs derived from long cDNAs followed by sequencing of the entire cDNAs provided indispensable information for the precise annotation of genes on the genome together with ESTs derived from short cDNAs... - Characterization of a human homolog (BACH1) of the mouse Bach1 gene encoding a BTB-basic leucine zipper transcription factor and its mapping to chromosome 21q22.1M Ohira
Laboratory of Gene Structure, Kazusa DNA Research Institute, Chiba, Japan
Genomics 47:300-6. 1998..Both the prospective function and the chromosomal location suggest that this gene may be a DS candidate gene, contributing to certain DS phenotypes, and is possibly involved in certain features of monosomy 21... - Identification of three novel non-classical cadherin genes through comprehensive analysis of large cDNAsD Nakajima
Department of Human Gene Research, Kazusa DNA Research Institute, 1532 3 Yana, Kisarazu, 292 0812, Chiba, Japan
Brain Res Mol Brain Res 94:85-95. 2001.... - Characterization of long cDNA clones from human adult spleenA Hattori
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 7:357-66. 2000..The results indicated that spleen could be used as an additional source of human long cDNAs to complement the list of human genes... - HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing projectR Kikuno
Kazusa DNA Research Institute, 1532 3 Yana, Kisarazu, Chiba 292 0812, Japan
Nucleic Acids Res 28:331-2. 2000..HUGE is available through the WWW at http://www.kazusa.or.jp/huge.. - Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1 (supplement)T Nagase
Kazusa DNA Research Institute, Chiba, Japan
DNA Res 3:43-53. 1996 - Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1T Nagase
Kazusa DNA Research Institute, Chiba, Japan
DNA Res 3:17-24. 1996..Another novel repeat composed of alternating Arg and Glu was identified in KIAA0182. Northern hybridization analysis demonstrated that 10 genes are expressed in a cell- or tissue-specific manner... - Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1 (supplement)N Nomura
Kazusa DNA Research Institute, Chiba, Japan
DNA Res 1:251-62. 1994 - Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated librariesNoriko Okazaki
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan
DNA Res 10:167-80. 2003..Notably, a homology search against the public database indicated that 4 out of 13 novel cDNA clones were homologous to the disease-related genes... - Identification of a novel human gene containing the tetratricopeptide repeat domain from the Down syndrome region of chromosome 21M Ohira
Laboratory of Gene Structure 1, Kazusa DNA Research Institute, Chiba, Japan
DNA Res 3:9-16. 1996..The TPRD gene, the novel gene which was proved to be in the 1.6-Mb region and to have the interesting features described above, is a candidate for genes responsible for the DS phenotypes... - Identification and characterization of a new gene physically linked to the ATM geneT Imai
Genome Research Group, National Institute of Radiological Sciences, Chiba, Japan
Genome Res 6:439-47. 1996..It may be possible to propose the idea that the promoter region could be shared by both housekeeping genes and that each gene could influence the expression of the other... - Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clonesDaisuke Nakajima
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 9:99-106. 2002..The total length of the resultant extensions at amino- and carboxy-terminals of KIAA gene products reached 97,000 and 7,216 amino acid residues, respectively, and various protein domains were found in these extended portions... - Influence of the 3'-UTR-length of mKIAA cDNAs and their sequence features to the mRNA expression level in the brainNoriko Okazaki
Kazusa DNA Research Institute, Kazusa Kamatari, Kisarazu, Chiba, Japan
DNA Res 12:181-9. 2005..Furthermore, we searched sequence elements in the 3'-UTR possibly related with their expression and found some candidates regarding the brain-specific expression... - A comprehensive approach for establishment of the platform to analyze functions of KIAA proteins II: public release of inaugural version of InGaP database containing gene/protein expression profiles for 127 mouse KIAA genes/proteinsHisashi Koga
Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
DNA Res 11:293-304. 2004..This database will be a resource for the neuroscience community by seamlessly integrating the genomic and proteomic information about the mouse KIAA genes/proteins... - Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated librariesNoriko Okazaki
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan
DNA Res 11:205-18. 2004..Furthermore, 3 out of 4 proteins encoded in the novel cDNA clones showed moderate sequence similarity with human KIAA proteins, thus we could obtain new members of KIAA protein families through our mouse cDNA projects... - [Comprehensive analysis of human large cDNAs which directs the synthesis of large proteins]Osamu Ohara
Tanpakushitsu Kakusan Koso 47:991-6. 2002 - HUGE: a database for human KIAA proteins, a 2004 update integrating HUGEppi and ROUGEReiko Kikuno
Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba, 292 0818, Japan
Nucleic Acids Res 32:D502-4. 2004..ROUGE summarizes the results of computer-assisted analyses of approximately 1000 mouse homologues of human large cDNAs that we identified... - Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted methodOsamu Ohara
Department of Human Gene Research, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan
DNA Res 9:47-57. 2002..Taking all these results together, we here conclude that this new method for the construction of size-fractionated cDNA libraries makes it possible to analyze cDNAs efficiently and comprehensively... - HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing projectReiko Kikuno
Kazusa DNA Research Institute, 1532 3 Yana, Kisarazu, Chiba 292 0812, Japan
Nucleic Acids Res 30:166-8. 2002..More interestingly, in some cases, the ability to compare cDNA with genomic sequences allows us to identify candidate sites of RNA editing. HUGE is available on the World Wide Web at http://www.kazusa.or.jp/huge... - A human homolog of the mitochondrial protein import receptor Mom19 can assemble with the yeast mitochondrial receptor complexN Seki
Kazusa DNA Research Institute, Chiba, Japan
FEBS Lett 375:307-10. 1995..When synthesized in vitro, the human Mom19 homolog is targeted to isolated yeast mitochondria and specifically associates with the outer membrane receptor complex, suggesting that indeed a mitochondrial import receptor was identified... - Characterization of long cDNA clones from human adult spleen. II. The complete sequences of 81 cDNA clonesHiroyuki Jikuya
Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Chiba 292 0818, Japan
DNA Res 10:49-57. 2003.... - Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated librariesNoriko Okazaki
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan
DNA Res 11:127-35. 2004..Homology and/or motif search against public databases revealed new domains and/or motifs in 26 mFLJ gene products which provide additional speculation regarding the function of FLJ genes... - Complete sequencing and characterization of 21,243 full-length human cDNAsToshio Ota
Helix Research Institute, 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan
Nat Genet 36:40-5. 2004..About one-fourth of them (1,378) showed a clear pattern of splicing. The distribution of GC content of noncoding cDNAs was narrow and had a peak at approximately 42%, relatively low compared with that of protein-coding cDNAs... - Preparation of a set of expression-ready clones of mammalian long cDNAs encoding large proteins by the ORF trap cloning methodDaisuke Nakajima
Department of Human Gene Research, Kazusa DNA Research Institute, Kazusa-Kamatari, Kisarazu, Chiba, Japan
DNA Res 12:257-67. 2005.... - Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated librariesNoriko Okazaki
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan
DNA Res 9:179-88. 2002..The average size of the 100 cDNA sequences reached 5.1 kb and that of mouse KIAA-homologous proteins predicted from these cDNAs was 989 amino acid residues... 
The novel protein complex with SMARCAD1/KIAA1122 binds to the vicinity of TSSNoriko Okazaki
Laboratory of Medical Genomics, Department of Human Genome Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292 0818, Japan
J Mol Biol 382:257-65. 2008..From these findings, we propose a novel model for gene regulation via the SMARCAD1/KIAA1122 protein complex...- Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated librariesNoriko Okazaki
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan
DNA Res 10:35-48. 2003..Furthermore, we successfully mapped all the mouse KIAA cDNAs along the genome using a recently published mouse genome draft sequence... - KAP1-independent transcriptional repression of SCAN-KRAB-containing zinc finger proteinsYasuhide Itokawa
Department of Human Genome Research, Kazusa DNA Research Institute, 2 6 7 Kazusa Kamatari, Kisarazu, Chiba 292 0818, Japan
Biochem Biophys Res Commun 388:689-94. 2009..These results provide new insight into the functions of the members of the KRAB-ZNF family... - Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteinsKei Yamaguchi
Laboratory of Human Gene Research, Department of Human Genome Research, Kazusa DNA Research Institute, Chiba, Japan
Methods Mol Biol 577:121-31. 2009..Herein, we demonstrated that the method allows analysis of the intracellular protein stability as regulated by protein-degradation signals or an exogenously expressed E3 ubiquitin ligase... - Genome-wide expression analysis of mouse liver reveals CLOCK-regulated circadian output genesKatsutaka Oishi
Clock Cell Biology Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Central 6, 1 1 1 Higashi, Tsukuba, Ibaraki 305 8566, Japan
J Biol Chem 278:41519-27. 2003.... - Phosphorylation of clock protein PER1 regulates its circadian degradation in normal human fibroblastsKoyomi Miyazaki
Clock Cell Biology Group, IBRF Institute for Biological Resource and Function, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba Central 6, 1 1 1, Higashi, Tsukuba, 305 8566, Japan
Biochem J 380:95-103. 2004..These findings indicate that circadian hPER1 degradation through a proteasomal pathway can be regulated through phosphorylation by CKI, but not by subcellular localization... - Dynamic and coordinated expression profile of dbl-family guanine nucleotide exchange factors in the developing mouse brainMasato Yoshizawa
Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe cho, 606 8501 Kyoto, Japan
Gene Expr Patterns 3:375-81. 2003..Our study reveals the dynamic and coordinated expression profiles of the Dbl-GEFs and provides a basic framework for understanding the function of GEFs in neural development... - Genetic linkage map of the house musk shrew, Suncus murinus, constructed with PCR-based and RFLP markersSamuel Adjei
Laboratory of Animal Genetics, Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan
Exp Anim 57:129-34. 2008..The new map comprises 42 markers that are distributed into 12 linkage groups, two of which are assigned to chromosomes, and spans 403.5 cM with an average inter-marker distance of 13.5 cM... - Identification of new human mastermind proteins defines a family that consists of positive regulators for notch signalingSey En Lin
Department of Molecular and Tumor Pathology, Chiba University Graduate School of Medicine, 1 8 1 Inohana, Chuo Ku, Chiba 260 8670, Japan
J Biol Chem 277:50612-20. 2002..The multiplicity of Mam proteins in the mammalian system may help provide divergence to the strength of the Notch signals in different cell types... - Smurf1 directly targets hPEM-2, a GEF for Cdc42, via a novel combination of protein interaction modules in the ubiquitin-proteasome pathwayKei Yamaguchi
Graduate School of Science and Technology, Chiba University, 648 Matsudo, Matsudo, Chiba 271 8510, Japan
Biol Chem 389:405-13. 2008..Our discovery that hPEM-2 is, in addition to RhoA, a target protein of Smurf1 suggests that Smurf1 plays a crucial role in the spatiotemporal regulation of Rho GTPase family members... - Heterotrimeric G protein betagamma subunits stimulate FLJ00018, a guanine nucleotide exchange factor for Rac1 and Cdc42Hiroshi Ueda
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1 1, Gifu 501 1193, Japan
J Biol Chem 283:1946-53. 2008..These results provide evidence for a signaling pathway by which G(i)-coupled receptor specifically induces Rac and Cdc42 activation through direct interaction of Gbetagamma with FLJ00018... - The CAP-Gly domain of CYLD associates with the proline-rich sequence in NEMO/IKKgammaKohei Saito
RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
Structure 12:1719-28. 2004..Therefore, CAP-Gly is likely to be a novel proline-rich sequence binding domain with a mechanism different from that of the SH3 domain... - Utilization of mammalian cells for efficient and reliable evaluation of specificity of antibodies to unravel the cellular function of mKIAA proteinsAkiyuki Ozaki
Department of Biotechnology, Institute of Research and Innovation, 1201 Takada, Kashiwa, Chiba 277-0861, Japan
Gene 360:35-44. 2005..Importantly, these methods allow us to detect potential posttranslational modification of the mKIAA/KIAA proteins and to predict their biological function based on their subcellular localization... - Alternative splice variants encoding unstable protein domains exist in the human brainKeiichi Homma
Laboratory of Gene Product Informatics, Center for Information Biology DNA Data Bank of Japan, National Institute of Genetics, Research Organization of Information and Systems, Shizuoka 411 8540, Japan
J Mol Biol 343:1207-20. 2004..We propose that most unstable proteins encoded by alternative splice variants lack normal functions and are an evolutionary dead-end...