Naoyuki Miyashita

..The purpose of this study was to clarify the prevalence and clinical characteristics of macrolide-resistant M. pneumoniae in adolescent patients with community-acquired pneumonia...

..In the present study, we evaluated the accuracy and usefulness of the JRS scoring system for diagnosing atypical pneumonia in different age groups...

..In addition, to clarify the strategy for treatment of NHCAP, we investigated the role of atypical pathogens...

..To determine the efficacy of macrolides against MR M. pneumoniae pneumonia, microbiological and clinical efficacies were compared during the antibiotic treatment...

..Mycoplasma pneumoniae is one of the most common causes of community-acquired pneumonia (CAP). This infection occurs predominantly in school-aged children and younger adults, but may also occur in the elderly...

..Our multiplex PCR assay could potentially be used as a diagnostic and epidemiological tool. Further prospective studies are needed to establish its clinical usefulness...

..The sensitivity and specificity of the MySet test were 100% and 92.9%, respectively. The MySet test is rapid and simple to use and is thought to be a useful tool for the selection of appropriate antibiotic therapy...

..To evaluate an enzyme immunoassay (EIA) (AniLab C. pneumoniae) for detecting anti-Chlamydophila pneumoniae-specific IgM antibody, by comparing it with an ELISA, Hitazyme C. pneumoniae, and a micro-immunofluorescence (MIF) test...

..The usefulness of sputum Gram stain and culture in guiding microbiological diagnosis of community-acquired pneumonia (CAP) is controversial. We evaluated the role of sputum examination at a university teaching hospital...

..No difference was detected for any of the agents tested, indicating that the 7.5-kb common plasmid is unrelated to the drug resistance of C. trachomatis...

..The study evaluated a newly developed ELISA (Hitazyme Chlamydophila pneumoniae) for detecting anti-C. pneumoniae-specific IgM antibody, by comparing the ELISA assay to a microimmunofluorescence (MIF) test and immunoblotting...

..5% and 90.9%, respectively. Our results indicated that the differentiation of pneumonia in the JRS guidelines is useful for the diagnosis of M. pneumoniae pneumonia, but difficult to apply to the diagnosis of C. pneumoniae pneumonia...

..pneumoniae acute infections and persistent ones or nasopharyngeal carriage. In addition, the detection of C. pneumoniae DNA in PBMCs does not seem to be a suitable method for the diagnosis of acute respiratory C. pneumoniae infections...

..pneumoniae and Haemophilus influenzae was higher among the hospitalized patients. Recognition of these results will allow prompt and appropriate antimicrobial therapy to be provided using Japanese CAP guidelines...

..Since repeated infection can certainly be documented in many clinical settings, persistence is thought to also play a role...

..Physicians and other health care providers in long-term care facilities should consider C. pneumoniae in the differential diagnosis of an outbreak of respiratory infection...

..pneumoniae pneumonia from Streptococcus pneumoniae pneumonia...

..To investigate the in vitro activity of cethromycin, a new ketolide, against Chlamydia pneumoniae...

..These data indicate that the higher C. pneumoniae and C. trachomatis seroprevalence among HIV-infected subjects is probably due to an HIV risk factor, such as promiscuous sexual behavior, rather than to HIV infection itself...

..To investigate the prevalence of asymptomatic infection with Chlamydia pneumoniae in subjectively healthy adults...

..No difference was detected for any of the antibiotics tested, indicating that the 7.5-kb common plasmid is unrelated to the drug susceptibility of C. trachomatis...

..These results appear to indicate that telithromycin is an effective antibiotic that should play some role in the treatment of respiratory tract infections caused by C. pneumoniae...

..031 to 0.125 microg/ml. Sitafloxacin had an excellent therapeutic effect on experimental Chlamydia psittaci pneumonia and was more potent than tosufloxacin, ofloxacin, and ciproflxacin, although slightly less potent than sparfloxacin...

..To clarify the endemic and epidemic status of Chlamydia pneumoniae in Japan, the incidence of anti-C pneumoniae antibody was evaluated over a period of 10 years...

..The minimal chlamydiacidal concentration of moxifloxacin ranged from 0.031 to 0.125 microg/ml. These results suggest that moxifloxacin has potential effects against Chlamydia species...

..To investigate the clinical presentation of community-acquired Chlamydia pneumoniae pneumonia in adults...

..In Japan, macrolide-resistant M. pneumoniae infections are common in pediatric patients but not in adults. However, physicians should pay attention to macrolide-resistant M. pneumoniae not only in children but also in adults...

..pneumoniae infection may cause persistent cough in adults. Furthermore, these data also indicate that it may be necessary to eradicate the organism when C. pneumoniae is detected by culture and/or PCR in patients with persistent cough...

..The delayed administration of adequate antibiotics may contribute to the severity of M. pneumoniae pneumonia. Early corticosteroid therapy with adequate antibiotics should be considered...

..If there is a correlation of three or four parameters in the JRS scoring system, chest computed tomography (CT) findings are helpful for the presumptive diagnosis of M. pneumoniae pneumonia...

..To prevent and control the outbreak, we conducted a survey of the laboratory staff and their family members, and we investigated the clinical features of adult pertussis...

..pneumoniae infections. Further studies are needed, to determine an appropriate cut-off level and the possible causes of the false-positive results in the ELISA kit, such as other underlying conditions...

..In conclusion, both blood assays seemed to be more useful than TST for the identification of patients with active TB disease. However, neither of the 2 blood tests appears to provide any certainty regarding the cure of infection...

..To investigate the characteristic clinical features of chronic necrotizing pulmonary aspergillosis (CNPA) as a complication of pulmonary Mycobacterium avium complex (MAC) disease...

..Although the positive rate on QFT-2G test decreased with age in adults, it is thought to be a useful supportive diagnostic method for active TB disease compared to the TST, except in younger patients aged < or =9 years old...

..pneumoniae pneumonia concomitant with other bacteria. If the patient is less than 60 years old and some guideline headings are excluded, we think it would be possible to distinguish between C. pneumoniae and bacterial pneumonia...

..Histopathological examination of transbronchial biopsy specimens demonstrated cryptococcal organisms. After fluconazole therapy for 4 months, the infiltrate had decreased in size and the bronchial polypoid lesions had disappeared...

..We report a rare case with a solitary nodule caused by pulmonary Mycobacterium intracellulare infectious disease which is indicative of lung cancer...

..However, there was an overlap in the IFN-gamma levels between active TB disease and LTBI groups. Therefore, it would be difficult to use the QFT-2G test to completely discriminate active TB disease from LTBI...

..Therefore, we studied the usefulness of two commercial IFN-gamma assays (QuantiFERON TB-2G (QFT-2G) and T-SPOT.TB tests) for monitoring clinical efficacy...

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..Although the ATS has not yet recommended routine drug susceptibility testing of CAM, we believe that drug susceptibility testing of CAM should be performed before the initial treatment is undertaken for pulmonary MAC disease...

..We have reported a peculiar case of pulmonary M. intracellulare disease, detected at the onset of left secondary pneumothorax caused by pulmonary emphysema, which required differentiation from pulmonary adenocarcinoma...

..To evaluate clinical findings of patients with a solitary pulmonary nodule in Japan caused by pulmonary Mycobacterium avium complex (MAC) disease...

..It can be measured with on- and off-line methods. In the latter, sample storage is possible; this is useful in Japan, because the number of facilities in which this instrument has been installed is limited...

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..As the causative pathogen, Mycobacterium avium complex (MAC) disease should be considered in the differential diagnosis of a rapidly increasing solitary nodule through this peculiar case of pulmonary MAC disease...

..We must perform the acid-fast bacilli examination for patients who have a fever and continuous cough, and antituberculous drugs should be administered as soon as possible when the results are positive...

..An aging population, increased use of immunosuppressive therapy and an increase in patients with risk factors for tuberculosis have induced changes in the clinical features of pulmonary tuberculosis in Japan...

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..pneumoniae pneumonia is rarely encountered, although self-limiting upper respiratory tract infections due to C. pneumoniae are common. Thus, most self-limiting C. pneumoniae pneumonia may be missed when symptoms are minimal...

..Then, the specificity of new MK assay was improved by using improved alkaline pretreatment reagent, without decreasing the sensitivity. Thus, the present findings indicate that the new MK assay is clinically quite useful...

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..Because penicillin antibiotics were administered for a long time for all patients after obtaining a definite diagnosis, the prognosis was good and there were no relapse...

..Our results indicated that C. pneumoniae may not be the primary cause of community-acquired pneumonia but it might descript the normal clearance mechanisms, enabling other pathogens to invade...

..Finally, to measure plasma beta-glucan levels accurately, non-specific reactions should be excluded as possible by further improvement of measurement methods...

..Concerning treatment, although corticosteroid therapy was administered, his clinical symptoms and abnormal chest shadow have not changed...

..To evaluate the clinical utility of the T-SPOT.TB test for patients with indeterminate results on the QFT-2G test...

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..Smear test of the resected tissue was positive for acid-fast bacilli and culture was positive for mycobacteria, which was identified as Mycobacterium kansasii. There are a few case reports of solitary nodule due to M. kansasii...

..The urinary antigen detection kit for Streptococcus pneumoniae was tested...

..To evaluate transitional changes in QuantiFERON TB-2G (QFT-2G) test results in the serial testing on the same patients and to reevaluate the optimal threshold of positive response of QFT-2G test as a cure of TB infection...

..By so doing, unnecessary initiation of anti fugal therapy in response to non-specific reactions can be avoided. Further prospective and radical studies of non-specific reactions in the alkaline-kinetic assay are necessary...

..The QFT-2G test may be useful as a rapid supportive diagnostic method for pulmonary infection due to MK possessing the same MTB-specific antigen...

..We prospectively evaluated the usefulness of the QuantiFERON TB-2G (QFT-2G) compared to that of tuberculin skin test (TST) as a supportive method of diagnosing pulmonary tuberculosis (TB) without invasive examinations...

..Atypical pneumonia occupies an important position in community-acquired pneumonia. The aim of this study was to examine whether making a diagnosis of atypical pneumonia is possible based upon the Japanese Respiratory Society guidelines...

..To investigate the current status of patients with tuberculosis in Kawasaki Medical School Hospital which has closed tuberculosis isolation ward and review the nosocomial tuberculosis infection control...

..pneumoniae. Some Asian countries have their own guidelines for community-acquired pneumonia, but these are written in their own languages...

..We report this case because there have been no previous reports in the literature of infectious lung bulla caused by M. intracellulare although there have been several reports dealing with lung bulla caused by Mycobacterium tuberculosis...

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..However, early diagnosis using bronchoscopy and early treatment seems to be useful, when Mycobacterium tuberculosis is not detected in the sputum...

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..to analyze MTB-specific T-cells in pleural effusion mononuclear cells by ELISpot is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of tuberculous pleuritis...

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..These data indicate that new treatment strategies may be necessary to eradicate the organism in patients prone to persistent infection...

..To evaluate the usefulness of QuantiFERON TB-2G (QFT-2G) for detecting latent tuberculosis infection (LTBI) in a contact investigation of health care workers...

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..bertholletiae) complicating an immunocompromised state was diagnosed. Ultra-thin bronchoscopy is useful to diagnose complex pulmonary infections and more research is needed to verify its clinical indications and utility...

..The analysis of MTB-specific T-cells in PEMCs by ELISPOT may be useful for rapid decision-making in anti-tuberculous treatment...

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..In addition, the JRS guidelines have recommended short-term usage of antibiotics of an appropriate dose and pathogen-specific treatment using rapid diagnostic methods if possible...

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..The results of PPK analysis with the dosage management also support the efficacy of GFLX...

..A larger number of cases should be evaluated to clarify the characteristics of these new kits...

..Herein, we have reported a rare case of histological diagnosis of pulmonary adenosquamous cell carcinoma with cavity formation...

..In spite of influenza vaccination inoculation in 20% of patients with influenza in 2004-2005 and 2005-2006, influenza spread. The prognosis of these patients, however, was comparatively good...