Hitoshi Sakaguchi

Summary

Affiliation: Kao Corporation
Country: Japan

Publications

  1. ncbi request reprint The relationship between CD86/CD54 expression and THP-1 cell viability in an in vitro skin sensitization test--human cell line activation test (h-CLAT)
    Hitoshi Sakaguchi
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi, Japan
    Cell Biol Toxicol 25:109-26. 2009
  2. ncbi request reprint Development of an in vitro skin sensitization test using human cell lines; human Cell Line Activation Test (h-CLAT). II. An inter-laboratory study of the h-CLAT
    H Sakaguchi
    Safety and Microbial Control Research Center, Kao Corporation, 2606 Akabane, Ichikai Maich, Haga Gun Tochigi 321 3497, Japan
    Toxicol In Vitro 20:774-84. 2006
  3. ncbi request reprint Prediction of preservative sensitization potential using surface marker CD86 and/or CD54 expression on human cell line, THP-1
    Hitoshi Sakaguchi
    Kao Corporation, Safety and Microbial Control Research Center, 2606 Akabane, Ichikai machi, Haga gun, Tochigi, 321 3497, Japan
    Arch Dermatol Res 298:427-37. 2007
  4. doi request reprint Validation study of the Short Time Exposure (STE) test to assess the eye irritation potential of chemicals
    Hitoshi Sakaguchi
    Kao Corporation, Safety Science Research Laboratories, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 25:796-809. 2011
  5. ncbi request reprint Predicting skin sensitization potential and inter-laboratory reproducibility of a human Cell Line Activation Test (h-CLAT) in the European Cosmetics Association (COLIPA) ring trials
    Hitoshi Sakaguchi
    Kao Corporation, Safety Science Research Laboratories, Haga gun, Tochigi, Japan
    Toxicol In Vitro 24:1810-20. 2010
  6. ncbi request reprint Role of TNF-alpha and extracellular ATP in THP-1 cell activation following allergen exposure
    Masaaki Miyazawa
    Kao Corporation, Safety Science Research Laboratories, Haga gun, Tochigi, Japan
    J Toxicol Sci 33:71-83. 2008
  7. ncbi request reprint Prediction of skin sensitization potency of chemicals by human Cell Line Activation Test (h-CLAT) and an attempt at classifying skin sensitization potency
    Yuko Nukada
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 26:1150-60. 2012
  8. ncbi request reprint Two-stage bottom-up tiered approach combining several alternatives for identification of eye irritation potential of chemicals including insoluble or volatile substances
    Kazuhiko Hayashi
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 26:1199-208. 2012
  9. doi request reprint Predictive performance for human skin sensitizing potential of the human cell line activation test (h-CLAT)
    Yuko Nukada
    Kao Corporation, Safety Science Research Laboratories, Tochigi 321 3497, Japan
    Contact Dermatitis 65:343-53. 2011
  10. doi request reprint An interlaboratory study of the short time exposure (STE) test using SIRC cells for predicting eye irritation potential
    Yutaka Takahashi
    Kao Corporation, Haga, Tochigi, Japan
    Cutan Ocul Toxicol 29:77-90. 2010

Detail Information

Publications24

  1. ncbi request reprint The relationship between CD86/CD54 expression and THP-1 cell viability in an in vitro skin sensitization test--human cell line activation test (h-CLAT)
    Hitoshi Sakaguchi
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi, Japan
    Cell Biol Toxicol 25:109-26. 2009
    ..These findings suggest that h-CLAT would be a good robust in vitro skin sensitization test...
  2. ncbi request reprint Development of an in vitro skin sensitization test using human cell lines; human Cell Line Activation Test (h-CLAT). II. An inter-laboratory study of the h-CLAT
    H Sakaguchi
    Safety and Microbial Control Research Center, Kao Corporation, 2606 Akabane, Ichikai Maich, Haga Gun Tochigi 321 3497, Japan
    Toxicol In Vitro 20:774-84. 2006
    ....
  3. ncbi request reprint Prediction of preservative sensitization potential using surface marker CD86 and/or CD54 expression on human cell line, THP-1
    Hitoshi Sakaguchi
    Kao Corporation, Safety and Microbial Control Research Center, 2606 Akabane, Ichikai machi, Haga gun, Tochigi, 321 3497, Japan
    Arch Dermatol Res 298:427-37. 2007
    ..These data suggest that h-CLAT, using THP-1 cells, may be able to predict the allergy potential of preservatives and possibility classify the potency of an allergen...
  4. doi request reprint Validation study of the Short Time Exposure (STE) test to assess the eye irritation potential of chemicals
    Hitoshi Sakaguchi
    Kao Corporation, Safety Science Research Laboratories, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 25:796-809. 2011
    ..Based on these findings, the STE test is a promising alternative eye irritation test that could be easily standardized...
  5. ncbi request reprint Predicting skin sensitization potential and inter-laboratory reproducibility of a human Cell Line Activation Test (h-CLAT) in the European Cosmetics Association (COLIPA) ring trials
    Hitoshi Sakaguchi
    Kao Corporation, Safety Science Research Laboratories, Haga gun, Tochigi, Japan
    Toxicol In Vitro 24:1810-20. 2010
    ..The data also support initiating formal pre-validation of the h-CLAT in order to fully understand the capabilities and limitations of the assay...
  6. ncbi request reprint Role of TNF-alpha and extracellular ATP in THP-1 cell activation following allergen exposure
    Masaaki Miyazawa
    Kao Corporation, Safety Science Research Laboratories, Haga gun, Tochigi, Japan
    J Toxicol Sci 33:71-83. 2008
    ..Therefore, we postulate that not only TNF-alpha but also extracellular ATP may contribute to cell activation following allergen stimulation, which might reflect the mechanism by which DCs respond to allergens...
  7. ncbi request reprint Prediction of skin sensitization potency of chemicals by human Cell Line Activation Test (h-CLAT) and an attempt at classifying skin sensitization potency
    Yuko Nukada
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 26:1150-60. 2012
    ..From these data, the h-CLAT values may be one of the useful tools to predict the allergic potency of chemicals...
  8. ncbi request reprint Two-stage bottom-up tiered approach combining several alternatives for identification of eye irritation potential of chemicals including insoluble or volatile substances
    Kazuhiko Hayashi
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 26:1199-208. 2012
    ..2), and Category 1 (Cat. 1)) for a wide range of test chemicals regardless of solubility...
  9. doi request reprint Predictive performance for human skin sensitizing potential of the human cell line activation test (h-CLAT)
    Yuko Nukada
    Kao Corporation, Safety Science Research Laboratories, Tochigi 321 3497, Japan
    Contact Dermatitis 65:343-53. 2011
    ....
  10. doi request reprint An interlaboratory study of the short time exposure (STE) test using SIRC cells for predicting eye irritation potential
    Yutaka Takahashi
    Kao Corporation, Haga, Tochigi, Japan
    Cutan Ocul Toxicol 29:77-90. 2010
    ..From the above data, excellent transferability, high between-laboratory reproducibility, and high predictive capacity of the STE test were observed in the interlaboratory study by 2 laboratories...
  11. ncbi request reprint Inter-laboratory study of short time exposure (STE) test for predicting eye irritation potential of chemicals and correspondence to globally harmonized system (GHS) classification
    Yutaka Takahashi
    Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan
    J Toxicol Sci 34:611-26. 2009
    ..0% at all laboratories). These results suggest that the STE test possessed easy transferability, reproducibility, good predictive performance...
  12. ncbi request reprint Production of IL-8 in THP-1 cells following contact allergen stimulation via mitogen-activated protein kinase activation or tumor necrosis factor-alpha production
    Yuko Nukada
    Kao Corporation, Safety Science Research Laboratories, Tochigi, Japan
    J Toxicol Sci 33:175-85. 2008
    ..A better understanding of the mechanism of DC activation in vitro might lead to the clarification of the in vivo skin sensitization mechanism...
  13. ncbi request reprint Role of MAPK signaling pathway in the activation of dendritic type cell line, THP-1, induced by DNCB and NiSO4
    Masaaki Miyazawa
    Kao Corporation, Safety Science Research Laboratories, Haga gun, Tochigi, Japan
    J Toxicol Sci 33:51-9. 2008
    ..These data indicate that both DNCB and NiSO(4) activate p38 MAPK and ERK, and thereby stimulate TNF-alpha release and phenotypic changes through the different signal transduction pathways...
  14. doi request reprint Development of the short time exposure (STE) test: an in vitro eye irritation test using SIRC cells
    Yutaka Takahashi
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai Maich, Haga Gun Tochigi 321 3497, Japan
    Toxicol In Vitro 22:760-70. 2008
    ..The STE test demonstrated itself to be simple, promising, have great potential, be of value, and to be an easily standardized alternative eye irritation test...
  15. ncbi request reprint Phenotypic alterations and cytokine production in THP-1 cells in response to allergens
    Masaaki Miyazawa
    Safety and Microbial Control Research Center, Kao Corporation, 2606 Akabane, Ichikai Maich, Haga Gun Tochigi 321 3497, Japan
    Toxicol In Vitro 21:428-37. 2007
    ..It is suggested that THP-1 cells, which could develop several DC-like properties, are suitable for identifying sensitizing potential of chemicals...
  16. ncbi request reprint Development of a new in vitro skin sensitization assay (Epidermal Sensitization Assay; EpiSensA) using reconstructed human epidermis
    Kazutoshi Saito
    Kao Corporation, Safety Science Research Laboratories, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 27:2213-24. 2013
    ..These results suggested that the RhE-based assay, termed epidermal sensitization assay (EpiSensA), could be an useful skin sensitization assay with a broad applicability domain including pre-/pro-haptens. ..
  17. ncbi request reprint Predictive performance of the human Cell Line Activation Test (h-CLAT) for lipophilic chemicals with high octanol-water partition coefficients
    Osamu Takenouchi
    Safety Science Research Laboratories, Kao Corporation, Japan
    J Toxicol Sci 38:599-609. 2013
    ..Our results demonstrated that the h-CLAT could predict sensitizing potential of various chemicals, which contain lipophilic chemicals using a large-scale chemical dataset. ..
  18. doi request reprint Data integration of non-animal tests for the development of a test battery to predict the skin sensitizing potential and potency of chemicals
    Yuko Nukada
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 27:609-18. 2013
    ..Our data not only demonstrates that h-CLAT can be part of a test battery with other methods but also supports the practical utility of a tiered system when h-CLAT and DPRA are the first screening methods for skin sensitization...
  19. ncbi request reprint Definition of the applicability domain of the Short Time Exposure (STE) test for predicting the eye irritation of chemicals
    Kazuhiko Hayashi
    Safety Science Research Laboratories, Kao Corporation, Ichikai machi, Haga gun, Tochigi, Japan
    Altern Lab Anim 41:157-71. 2013
    ..Overall, we are encouraged by the performance and improved accuracy of the STE test...
  20. doi request reprint The Short Time Exposure (STE) test for predicting eye irritation potential: intra-laboratory reproducibility and correspondence to globally harmonized system (GHS) and EU eye irritation classification for 109 chemicals
    Yutaka Takahashi
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 25:1425-34. 2011
    ..Based on the above results, STE test was considered to be a promising alternative method for assessing eye irritation that has high intra-laboratory reproducibility as well as an excellent predictability of eye irritation...
  21. ncbi request reprint A tiered approach combining the short time exposure (STE) test and the bovine corneal opacity and permeability (BCOP) assay for predicting eye irritation potential of chemicals
    Kazuhiko Hayashi
    Safety Science Research Laboratories, Kao Corporation, Tochigi, Japan
    J Toxicol Sci 37:269-80. 2012
    ..From these results, the tiered approach of combining the data analysis of the STE test and BCOP assay might be a promising alternative eye irritation test strategy...
  22. ncbi request reprint The relationship between CD86 and CD54 protein expression and cytotoxicity following stimulation with contact allergen in THP-1 cells
    Yuko Nukada
    Kao Corporation, 2606 Akabane, Haga gun, Tochigi 321 3497, Japan
    J Toxicol Sci 36:313-24. 2011
    ..Altogether, these results suggest that cytotoxicity induced by contact allergens may result in apoptosis and may also be stimulated in parallel with protein expression through an intracellular signal or signals...
  23. doi request reprint Development of an in vitro skin sensitization test based on ROS production in THP-1 cells
    Kazutoshi Saito
    Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai machi, Haga gun, Tochigi 321 3497, Japan
    Toxicol In Vitro 27:857-63. 2013
    ..These results indicated that the THP-1 cell-based ROS assay was a rapid and highly sensitive detection system able to predict skin sensitizing potentials and potency of chemicals...
  24. ncbi request reprint Depigmentation of the skin induced by 4-(4-hydroxyphenyl)-2-butanol is spontaneously re-pigmented in brown and black guinea pigs
    Yasutaka Kuroda
    Safety Science Research Laboratories, Kao Corporation
    J Toxicol Sci 39:615-23. 2014
    ..These data indicate that skin depigmentation is induced by the toxicity of HPB to epidermal melanocytes, and that the induced skin depigmentation can recover by terminating the application of HPB. ..