Toshihisa Ohtsuka

Summary

Affiliation: KAN Research Institute
Country: Japan

Publications

  1. ncbi request reprint Gas6, a new regulator of chondrogenic differentiation from mesenchymal cells
    Hiraku Motomura
    Department of Orthopaedic Surgery, Faculty of Medicine, University of Toyama, Toyama 930 0194, Japan
    Biochem Biophys Res Commun 357:997-1003. 2007
  2. pmc Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1
    Toshihisa Ohtsuka
    KAN Research Institute Inc, Kyoto 600 8815, Japan
    J Cell Biol 158:577-90. 2002
  3. pmc Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
    Etsuko Takao-Rikitsu
    KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
    J Cell Biol 164:301-11. 2004
  4. ncbi request reprint SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release
    Eiji Inoue
    KAN Research Institute, Kyoto 600 8815, Japan
    Neuron 50:261-75. 2006
  5. ncbi request reprint ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells
    Eiji Inoue
    KAN Research Institute, Kyoto 600 8815, Japan
    Genes Cells 11:659-72. 2006
  6. ncbi request reprint Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina
    Maki Deguchi-Tawarada
    KAN Research Institute, Kyoto 600 8815, Japan
    J Comp Neurol 495:480-96. 2006
  7. ncbi request reprint CAST2: identification and characterization of a protein structurally related to the presynaptic cytomatrix protein CAST
    Maki Deguchi-Tawarada
    KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
    Genes Cells 9:15-23. 2004
  8. ncbi request reprint Nectin-like molecule-1/TSLL1/SynCAM3: a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes
    Shigeki Kakunaga
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Cell Sci 118:1267-77. 2005
  9. ncbi request reprint Three-dimensional architecture of presynaptic terminal cytomatrix
    Lea Siksou
    INSERM U789, Ecole Normale Superieure, 75005 Paris, France
    J Neurosci 27:6868-77. 2007
  10. ncbi request reprint The active zone protein CAST directly associates with Ligand-of-Numb protein X
    Susumu Higa
    Department of Clinical and Molecular Pathology, Graduate School of Medicine Faculty of Medicine, University of Toyama, Toyama 930 0194, Japan
    Biochem Biophys Res Commun 354:686-92. 2007

Collaborators

  • Yoshimi Takai
  • Mitsutoshi Setou
  • Hung Teh Kao
  • Yuko Mimori-Kiyosue
  • Frank Grosveld
  • Akari Hagiwara
  • Shinya Nagamatsu
  • Antoine Triller
  • E D Gundelfinger
  • Yoshihiro Akimoto
  • H Y Gaisano
  • Niels Galjart
  • Maki Deguchi-Tawarada
  • Eiji Inoue
  • Isao Kitajima
  • Etsuko Takao-Rikitsu
  • Susumu Higa
  • Marie Inoue
  • Takashi Tokoro
  • Ikuko Yao
  • Ilya Grigoriev
  • Hiraku Motomura
  • Lea Siksou
  • Edwin P Kwan
  • Anna Akhmanova
  • Jeroen Demmers
  • Hiroshi Takagi
  • Gideon Lansbergen
  • Shigeki Kakunaga
  • Mica Ohara-Imaizumi
  • Sumiko Mochida
  • Wataru Ikeda
  • Maiko Inagaki
  • Shigeki Yuasa
  • Hideki Niimi
  • Ivan V Maly
  • Phebe S Wulf
  • Tomoki Chiba
  • Thomas Boudier
  • Anna Fejtova
  • Yoshiyuki Fukuda
  • Nobuhiro Morone
  • Ken Hatanaka
  • Showbu Sato
  • Laura Sheu
  • Paul Greengard
  • Hiroshi Ageta
  • Jean Pierre Lechaire
  • Kaoru Inokuchi
  • DaniĆ«l Splinter
  • Tomoaki Kahyo
  • Tomoatsu Kimura
  • Grant R MacGregor
  • Li Xie
  • Mauro Modesti
  • Keiji Tanaka
  • Philippe Rostaing
  • Casper C Hoogenraad
  • Serge Marty
  • Nanda Keijzer
  • Kazuhito Sugimori
  • Adriaan B Houtsmuller
  • Kosei Takeuchi
  • Yoko Nakamichi
  • Akira Mizoguchi
  • Satsuki Matsushima
  • Toshiteru Kikuta
  • Hayato Kawakami
  • Shinsuke Itoh
  • Shintaro Nagai
  • Chiyono Nishiwaki
  • Takashi Watanabe
  • Masakazu Takeuchi
  • Kenji Irie

Detail Information

Publications19

  1. ncbi request reprint Gas6, a new regulator of chondrogenic differentiation from mesenchymal cells
    Hiraku Motomura
    Department of Orthopaedic Surgery, Faculty of Medicine, University of Toyama, Toyama 930 0194, Japan
    Biochem Biophys Res Commun 357:997-1003. 2007
    ..Gas6 signaling activated the phosphorylation of ERK1/2, SAPK/JNK, and Akt, but not p38 MAPK. These results suggest that Gas6 negatively regulates chondrogenic differentiation, at least through the MAPK pathway...
  2. pmc Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1
    Toshihisa Ohtsuka
    KAN Research Institute Inc, Kyoto 600 8815, Japan
    J Cell Biol 158:577-90. 2002
    ..These results suggest that these CAZ proteins are at least partly transported on the same vesicles during synapse formation...
  3. pmc Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
    Etsuko Takao-Rikitsu
    KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
    J Cell Biol 164:301-11. 2004
    ..These results indicate that CAST serves as a key component of the CAZ structure and is involved in neurotransmitter release by binding these CAZ proteins...
  4. ncbi request reprint SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release
    Eiji Inoue
    KAN Research Institute, Kyoto 600 8815, Japan
    Neuron 50:261-75. 2006
    ..Furthermore, SAD-B phosphorylates the active zone protein RIM1 but not Munc13-1. These results suggest that mammalian SAD kinase presynaptically regulates neurotransmitter release...
  5. ncbi request reprint ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells
    Eiji Inoue
    KAN Research Institute, Kyoto 600 8815, Japan
    Genes Cells 11:659-72. 2006
    ..These results suggest that ELKS is involved in Ca(2+)-dependent exocytosis from PC12 cells at least partly via the RIM2-Munc13-1 pathway...
  6. ncbi request reprint Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina
    Maki Deguchi-Tawarada
    KAN Research Institute, Kyoto 600 8815, Japan
    J Comp Neurol 495:480-96. 2006
    ..Both proteins also localized in the vicinity of the presynaptic plasma membrane of conventional synapses in the retina. These results indicated that CAST and ELKS were novel components of the presynaptic apparatus of mouse retina...
  7. ncbi request reprint CAST2: identification and characterization of a protein structurally related to the presynaptic cytomatrix protein CAST
    Maki Deguchi-Tawarada
    KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
    Genes Cells 9:15-23. 2004
    ..Accordingly, the original CAST is tentatively re-named CAST1. These results indicate that CAST2 is a new component of the CAZ and, together with CAST1, may be involved in the formation of the CAZ structure...
  8. ncbi request reprint Nectin-like molecule-1/TSLL1/SynCAM3: a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes
    Shigeki Kakunaga
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
    J Cell Sci 118:1267-77. 2005
    ....
  9. ncbi request reprint Three-dimensional architecture of presynaptic terminal cytomatrix
    Lea Siksou
    INSERM U789, Ecole Normale Superieure, 75005 Paris, France
    J Neurosci 27:6868-77. 2007
    ..This 3D analysis reveals the morphological constraints exerted by the presynaptic molecular scaffold. SVs are tightly interconnected in the axonal bouton, and this network is preferentially connected to the AZ...
  10. ncbi request reprint The active zone protein CAST directly associates with Ligand-of-Numb protein X
    Susumu Higa
    Department of Clinical and Molecular Pathology, Graduate School of Medicine Faculty of Medicine, University of Toyama, Toyama 930 0194, Japan
    Biochem Biophys Res Commun 354:686-92. 2007
    ..Moreover, exogenously expressed LNX was partially colocalized with endogenous CAST in the axonal varicosities of cultured rat hippocampal neurons. These results suggest that CAST and LNX might form a protein complex in neurons...
  11. ncbi request reprint [Structure and function of the presynaptic active zones]
    Toshihisa Ohtsuka
    Department of Clinical and Molecular Pathology, Faculty of Medicine Graduate School of Medicine, University of Toyama, Sugitani 2630, Toyama 930 0194, Japan
    Seikagaku 78:979-86. 2006
  12. pmc ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence mi
    Mica Ohara-Imaizumi
    Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181 8611, Japan
    Mol Biol Cell 16:3289-300. 2005
    ..Moreover, attenuation of ELKS expression by small interfering RNA reduced the glucose-evoked insulin release. These data suggest that the CAZ-related protein ELKS functions in insulin exocytosis from pancreatic beta cells...
  13. pmc SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release
    Ikuko Yao
    Mitsubishi Kagaku Institute of Life Sciences MITILS, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
    Cell 130:943-57. 2007
    ..Thus, SCRAPPER has an essential function in regulating proteasome-mediated degradation of RIM1 required for synaptic tuning...
  14. ncbi request reprint CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta
    Gideon Lansbergen
    MGC Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
    Dev Cell 11:21-32. 2006
    ..We propose that LL5beta and ELKS can form a PIP3-regulated cortical platform to which CLASPs attach distal microtubule ends...
  15. ncbi request reprint Nectin-dependent localization of ZO-1 at puncta adhaerentia junctions between the mossy fiber terminals and the dendrites of the pyramidal cells in the CA3 area of adult mouse hippocampus
    Maiko Inagaki
    Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
    J Comp Neurol 460:514-24. 2003
    ..These results indicate that ZO-1 colocalizes with nectin and afadin at the PAs and that the nectin-afadin system is involved in the localization of ZO-1...
  16. ncbi request reprint Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labeling
    Akari Hagiwara
    Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki 444 8787, Japan
    J Comp Neurol 489:195-216. 2005
    ..These results support the involvement of the t-SNARE proteins in exocytotic fusion in the AZ and the role of CAST in specialization of the membrane domain for the AZ...
  17. ncbi request reprint Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscular junctions
    Takashi Tokoro
    Department of Clinical and Molecular Pathology, Graduate School of Medicine Faculty of Medicine, University of Toyama, Sugitani, Toyama, Japan
    Neuroreport 18:313-6. 2007
    ..These results suggest that at least ELKS and Piccolo, but not CAST, are components of neuromuscular junction synapses in the peripheral nervous system...
  18. ncbi request reprint Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue of exocytotic defects in Munc13-1 deficient pancreatic beta-cells
    Edwin P Kwan
    Department of Physiology, University of Toronto, Toronto, Ontario, Canada
    Diabetes 56:2579-88. 2007
    ..We had reported that haplodeficient Munc13-1(+/-) mouse islet beta-cells exhibited reduced insulin secretion, causing glucose intolerance. Munc13-1 binds Epac2 and Rim2, but their functional interactions remain unclear...
  19. ncbi request reprint Rab6 regulates transport and targeting of exocytotic carriers
    Ilya Grigoriev
    Department of Cell Biology, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands
    Dev Cell 13:305-14. 2007
    ..Our data demonstrate that although Rab6 is not essential for secretion, it controls the organization of exocytosis within the cellular space...