Kazuhiro Shiozaki


Affiliation: Kagoshima University
Country: Japan


  1. Honda A, Chigwechokha P, Kamada Futagami Y, Komatsu M, Shiozaki K. Unique nuclear localization of Nile tilapia (Oreochromis niloticus) Neu4 sialidase is regulated by nuclear transport receptor importin ?/?. Biochimie. 2018;149:92-104 pubmed publisher
    ..In summary, tilapia Neu4 is a unique sialidase localized at nucleus and its transport system into nucleus is regulated by importin. ..
  2. Chigwechokha P, Komatsu M, Itakura T, Shiozaki K. Nile Tilapia Neu3 sialidases: molecular cloning, functional characterization and expression in Oreochromis niloticus. Gene. 2014;552:155-64 pubmed publisher
    ..Based on these results, tilapia Neu3 exploration is an important step towards full understanding of a more comprehensive picture of Neu3 sub-family of proteins in fish. ..
  3. Chigwechokha P, Tabata M, Shinyoshi S, Oishi K, Araki K, Komatsu M, et al. Recombinant sialidase NanA (rNanA) cleaves α2-3 linked sialic acid of host cell surface N-linked glycoprotein to promote Edwardsiella tarda infection. Fish Shellfish Immunol. 2015;47:34-45 pubmed publisher
    ..Together, these results suggest that E. tarda may employ endogenous NanA to desialylate α2-3 glycoproteins on host cells, thus revealing one of the potential binding molecules during infection. ..
  4. Shiozaki K, Harasaki Y, Fukuda M, Yoshinaga A, Ryuzono S, Chigwechokha P, et al. Positive regulation of myoblast differentiation by medaka Neu3b sialidase through gangliosides desialylation. Biochimie. 2016;123:65-72 pubmed publisher
    ..These results suggested that Neu3b sialidase is a positive regulator for myoblast differentiation, similar with mammalian cytosolic sialidase Neu2. ..
  5. Yoshinaga A, Kajiya N, Oishi K, Kamada Y, Ikeda A, Chigwechokha P, et al. NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation. Eur J Pharmacol. 2016;782:21-9 pubmed publisher
    ..NEU3 inhibitory effect of naringin induced GM3 accumulation in HeLa and A549 cells, leading the attenuation of EGFR/ERK signaling accompanied with a decrease in cell growth. ..
  6. Shiozaki K, Ryuzono S, Matsushita N, Ikeda A, Takeshita K, Chigwechokha P, et al. Molecular cloning and biochemical characterization of medaka (Oryzias latipes) lysosomal neu4 sialidase. Fish Physiol Biochem. 2014;40:1461-72 pubmed publisher
    ..5 dpf. On the other hand, neu3b expression was drastically increased after hatching, suggesting that each sialidase may play a different role in embryonic development. ..
  7. Shinyoshi S, Kamada Y, Matsusaki K, Chigwechokha P, Tepparin S, Araki K, et al. Naringenin suppresses Edwardsiella tarda infection in GAKS cells by NanA sialidase inhibition. Fish Shellfish Immunol. 2017;61:86-92 pubmed publisher
    ..tarda in GAKS cells was declined by naringenin treatment. The present study exhibited the possibility of naringenin as an effective ingredient in fish diet for the inhibition of E. tarda infection. ..
  8. Ryuzono S, Takase R, Kamada Y, Ikenaga T, Chigwechokha P, Komatsu M, et al. Suppression of Neu1 sialidase delays the absorption of yolk sac in medaka (Oryzias latipes) accompanied with the accumulation of ?2-3 sialo-glycoproteins. Biochimie. 2017;135:63-71 pubmed publisher
    ..Our results suggest that medaka Neu1 could be crucial for embryonic development through the degradation of yolk sac nutrition. ..
  9. Shiozaki K, Takeshita K, Ikeda M, Ikeda A, Harasaki Y, Komatsu M, et al. Molecular cloning and biochemical characterization of two novel Neu3 sialidases, neu3a and neu3b, from medaka (Oryzias latipes). Biochimie. 2013;95:280-9 pubmed publisher
    ..These results and phylogenetic analysis suggested that the medaka neu3a is an evolutionally conserved sialidase with regard to enzymatic properties, whereas neu3b is likely to have originally evolved in medaka. ..

More Information


  1. Ryuzono S, Takase R, Oishi K, Ikeda A, Chigwechokha P, Funahashi A, et al. Lysosomal localization of Japanese medaka (Oryzias latipes) Neu1 sialidase and its highly conserved enzymatic profiles with human. Gene. 2016;575:513-523 pubmed publisher
    ..Moreover, the present study showed the possibility of medaka as a model animal of human NEU1 sialidase. ..