Kaoru Takegawa

Summary

Affiliation: Kagawa University
Country: Japan

Publications

  1. doi request reprint Identification and characterization of a gene required for alpha1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe
    Yuka Ikeda
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa, Japan
    FEMS Yeast Res 9:115-25. 2009
  2. pmc Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761 0795, Japan
    Microbiology 153:2753-64. 2007
  3. ncbi request reprint Cloning, sequencing, and expression of Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase in Escherichia coli
    K Takegawa
    Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Miki cho, Japan
    Arch Biochem Biophys 338:22-8. 1997
  4. ncbi request reprint Vesicle-mediated protein transport pathways to the vacuole in Schizosaccharomyces pombe
    Kaoru Takegawa
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Cell Struct Funct 28:399-417. 2003
  5. ncbi request reprint Heterologous expression and characterization of Schizosaccharomyces pombe vacuolar carboxypeptidase Y in Saccharomyces cerevisiae
    Kaoru Takegawa
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Curr Genet 42:252-9. 2003
  6. ncbi request reprint Identification of a SNARE protein required for vacuolar protein transport in Schizosaccharomyces pombe
    Kaoru Takegawa
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Japan
    Biochem Biophys Res Commun 311:77-82. 2003
  7. ncbi request reprint Vacuolar protein sorting receptor in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Microbiology 152:1523-32. 2006
  8. doi request reprint The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity
    Ken Ichi Yoritsune
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan
    FEBS Lett 587:917-21. 2013
  9. doi request reprint Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation
    Hiroyuki Mukaiyama
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Microbiology 155:3816-26. 2009
  10. ncbi request reprint Loss of a GPI-anchored membrane protein Aah3p causes a defect in vacuolar protein sorting in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa, Japan
    Biosci Biotechnol Biochem 71:623-6. 2007

Collaborators

Detail Information

Publications84

  1. doi request reprint Identification and characterization of a gene required for alpha1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe
    Yuka Ikeda
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa, Japan
    FEMS Yeast Res 9:115-25. 2009
    ..An Omh1-GFP fusion protein was found to be localized in the Golgi apparatus. These results indicate that Omh1p plays a major role in extending alpha1,2-linked mannose in the O-glycan pathway in S. pombe...
  2. pmc Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761 0795, Japan
    Microbiology 153:2753-64. 2007
    ..Phenotypic analyses suggested that Sst2p is a class E Vps protein. Taken together, these results suggest that sorting into multivesicular bodies is dependent on class E Vps proteins, including Sst2p, in Sch. pombe...
  3. ncbi request reprint Cloning, sequencing, and expression of Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase in Escherichia coli
    K Takegawa
    Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Miki cho, Japan
    Arch Biochem Biophys 338:22-8. 1997
    ..N-terminal sequence analysis revealed that native and recombinant Endo-A have the same N-terminus. Recombinant and native Endo-A also showed very similar optimum pH profiles and transglycosylation activity...
  4. ncbi request reprint Vesicle-mediated protein transport pathways to the vacuole in Schizosaccharomyces pombe
    Kaoru Takegawa
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Cell Struct Funct 28:399-417. 2003
    ..pombe. This review focuses on the recent advances in vesicle-mediated protein transport to the vacuole in S. pombe...
  5. ncbi request reprint Heterologous expression and characterization of Schizosaccharomyces pombe vacuolar carboxypeptidase Y in Saccharomyces cerevisiae
    Kaoru Takegawa
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Curr Genet 42:252-9. 2003
    ..These results suggested that there are two different binding sites for SpCPY and ScCPY on Vps10p and that the binding of SpCPY to Vps10p interferes with the binding of a ScCPY-invertase fusion protein...
  6. ncbi request reprint Identification of a SNARE protein required for vacuolar protein transport in Schizosaccharomyces pombe
    Kaoru Takegawa
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Japan
    Biochem Biophys Res Commun 311:77-82. 2003
    ..These results showed that Fsv1p is mainly involved in vesicle-mediated protein transport between the Golgi and vacuole in S. pombe cells...
  7. ncbi request reprint Vacuolar protein sorting receptor in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Microbiology 152:1523-32. 2006
    ..These results indicate that the vps26(+), vps29(+) and vps35(+) gene products are required for retrograde transport of SpVps10p from the prevacuolar compartment back to the Golgi in Sch. pombe cells...
  8. doi request reprint The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity
    Ken Ichi Yoritsune
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan
    FEBS Lett 587:917-21. 2013
    ..The recombinant Pvg1p transferred pyruvyl residues from phosphoenolpyruvate specifically to β-linked galactose...
  9. doi request reprint Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation
    Hiroyuki Mukaiyama
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Microbiology 155:3816-26. 2009
    ..Taking advantage of this partial sporulation ability of fission yeast, we sought evidence for the existence of a recycling system for nitrogen sources during starvation...
  10. ncbi request reprint Loss of a GPI-anchored membrane protein Aah3p causes a defect in vacuolar protein sorting in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa, Japan
    Biosci Biotechnol Biochem 71:623-6. 2007
    ..These results indicate that aah3Delta had a defect in the retrograde transport of Vps10p, and that Aah3p is the first S. pombe specific protein required for vacuolar protein sorting...
  11. doi request reprint Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma-membrane proteins in Schizosaccharomyces pombe
    Mai Nakase
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    J Cell Sci 123:1578-87. 2010
    ..These results suggest that MIPC has multiple functions not only in the maintenance of cell and vacuole morphology but also in vesicular trafficking in fission yeast...
  12. doi request reprint Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe
    Soracom Chardwiriyapreecha
    Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University, Ehime, Japan
    FEBS Lett 584:2339-45. 2010
    ..Our results show that under diverse growth conditions Avt5p is involved in vacuolar transport of a selective set of amino acids...
  13. ncbi request reprint Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Yeast 20:845-55. 2003
    ..These results are consistent with Vps33p being necessary for the morphogenesis of vacuoles and subsequent expression of vacuolar functions in Sz. pombe cells...
  14. ncbi request reprint Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe
    Yasuko Fujita
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa, Japan
    FEMS Yeast Res 6:883-7. 2006
    ..2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe...
  15. ncbi request reprint Development of a genetic transformation system using new selectable markers for fission yeast Schizosaccharomyces pombe
    Yasuko Fujita
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Yeast 22:193-202. 2005
    ..The new Sz. pombe host strain will be a useful tool for molecular genetic studies of Sz. pombe where multiple recombinant modifications or multiple mutations are needed...
  16. doi request reprint Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe
    Takao Ohashi
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6 10 1 Hakozaki, Fukuoka 812 8581, Japan
    J Biotechnol 150:348-56. 2010
    ....
  17. doi request reprint Structural analysis of α1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple α-galactosyltransferase genes disruptions
    Takao Ohashi
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Higashi ku, Fukuoka 812 8581, Japan
    Glycobiology 21:340-51. 2011
    ....
  18. ncbi request reprint Atg22p, a vacuolar membrane protein involved in the amino acid compartmentalization of Schizosaccharomyces pombe
    Naoko Sugimoto
    Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University, Matsuyama, Japan
    Biosci Biotechnol Biochem 75:385-7. 2011
    ..The uptake of several amino acids, such as lysine, histidine, and arginine, was impaired in atg22Δ cells. S. pombe Atg22p plays an important role in the compartmentalization of amino acids...
  19. ncbi request reprint Characterization of end4+, a gene required for endocytosis in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Yeast 21:867-81. 2004
    ....
  20. doi request reprint Ght2⁺ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    Appl Microbiol Biotechnol 97:4957-64. 2013
    ..These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose...
  21. ncbi request reprint CUE domain-containing protein Vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe
    Mai Nakase
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan
    Biosci Biotechnol Biochem 76:652-9. 2012
    ..Heterologous expression of the budding yeast VPS9 gene corrected the CPY mis-sorting defect in vps901Δ cells. These findings suggest that the VPS9 domain of Vps901p is required for vacuolar protein trafficking in S. pombe...
  22. doi request reprint Schizosaccharomyces pombe Pep12p is required for vacuolar protein transport and vacuolar homotypic fusion
    Akira Hosomi
    Department of Life Sciences, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    J Biosci Bioeng 112:309-14. 2011
    ..The S. pombe pep12Δ mutation phenocopies that of vps33Δ, suggesting that both Pep12p and Vps33p act at the same membrane fusion step in S. pombe, and both mutations cause vacuolar deficiency...
  23. doi request reprint Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Microbiology 154:830-41. 2008
    ..Furthermore, in fission yeast, ergosterol is important for plasma membrane structure and function and for localization of plasma membrane proteins...
  24. ncbi request reprint Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe
    Naotaka Tanaka
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Biochem Biophys Res Commun 330:813-20. 2005
    ..These results indicate that O-glycosylation initiated by Ogm proteins plays crucial physiological roles and can serve as a sorting determinant for protein transport of membrane glycoproteins in S. pombe...
  25. doi request reprint Ethanol-inducible gene expression using gld1 (+) promoter in the fission yeast Schizosaccharomyces pombe
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka, 812 8581, Japan
    Appl Microbiol Biotechnol 97:6835-43. 2013
    ..The pEG1 gene expression system is an effective tool for the production of heterologous proteins under glucose-limiting conditions, including medium containing glycerol as a carbon source. ..
  26. doi request reprint Identification of a galactose-specific flocculin essential for non-sexual flocculation and filamentous growth in Schizosaccharomyces pombe
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    Mol Microbiol 82:1531-44. 2011
    ..The newly identified flocculin Gsf2 in fission yeast was not only required for non-sexual flocculation but was also required for adhesion and filamentous growth through recognition of galactose residues on cell surface glycoconjugates...
  27. doi request reprint Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p
    Mai Nakase
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan
    Microbiology 158:659-73. 2012
    ..These results indicate that ubiquitination is an important determinant for localization and regulation of the Aat1p permease in S. pombe...
  28. ncbi request reprint Mitochondrial ABC transporter Atm1p is required for protection against oxidative stress and vacuolar functions in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Japan
    Biosci Biotechnol Biochem 69:2109-16. 2005
    ..GFP-tagged Atm1p was observed to be localized in the mitochondria. These data strongly suggest that fission yeast Atm1p was not only involved in protection against oxidative stress, but also played a role in vacuolar functions...
  29. ncbi request reprint An alpha-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe
    Tomotake Morita
    Department of Life Sciences, Faculty of Agriculture, Kagawa University
    Biosci Biotechnol Biochem 70:1454-63. 2006
    ..Taken together, these results indicate that Aah3p is a GPI-anchored protein and is required for cell and ascal wall integrity in S. pombe...
  30. ncbi request reprint Function analysis of conserved amino acid residues in a Mn(2+)-dependent protein phosphatase, Pph3, from Myxococcus xanthus
    Yumi Mori
    Department of Applied Biological Science, Kagawa University, Miki cho, Kagawa, Japan
    J Biochem 152:269-74. 2012
    ....
  31. doi request reprint Autophagy in the fission yeast Schizosaccharomyces pombe
    Hiroyuki Mukaiyama
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
    FEBS Lett 584:1327-34. 2010
    ..Most of the nitrogen used for de novo protein synthesis during sporulation derives from the autophagic protein degradation system. This review focuses on the recent advances in the role of autophagy in fission yeast...
  32. ncbi request reprint The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe
    Tomohiko Matsuzawa
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa, 761 0795, Japan
    Appl Microbiol Biotechnol 87:715-27. 2010
    ..We found that gld1 (+) was regulated by glucose repression and that it was derepressed in scr1 Delta and tup12 Delta strains...
  33. ncbi request reprint Vba2p, a vacuolar membrane protein involved in basic amino acid transport in Schizosaccharomyces pombe
    Naoko Sugimoto
    Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University, Matsuyama, Japan
    Biosci Biotechnol Biochem 74:2166-9. 2010
    ..A transient increase in lysine uptake under nitrogen starvation was lowered by this mutation. These findings suggest that Vba2p is involved in basic amino acid transport in S. pombe under diverse conditions...
  34. ncbi request reprint PXA domain-containing protein Pxa1 is required for normal vacuole function and morphology in Schizosaccharomyces pombe
    Akira Hosomi
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Japan
    Biosci Biotechnol Biochem 72:548-56. 2008
    ..Taken together, these results indicate that Pxa1 protein is required for normal vacuole function and morphology in S. pombe...
  35. doi request reprint Expression of budding yeast IPT1 produces mannosyldiinositol phosphorylceramide in fission yeast and inhibits cell growth
    Mai Nakase
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
    Microbiology 158:1219-28. 2012
    ..These results indicate that M(IP)(2)C is toxic to Schiz. pombe and that fission yeast possesses an unknown mechanism to effectively extrude toxic sphingolipids from cells...
  36. doi request reprint N- and O-linked oligosaccharides completely lack galactose residues in the gms1och1 mutant of Schizosaccharomyces pombe
    Takao Ohashi
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    Appl Microbiol Biotechnol 86:263-72. 2010
    ....
  37. ncbi request reprint The och1 mutant of Schizosaccharomyces pombe produces galactosylated core structures of N-linked oligosaccharides
    Takao Ohashi
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Japan
    Biosci Biotechnol Biochem 73:407-14. 2009
    ..The structural heterogeneity arose mainly from the addition of alpha1,2- and alpha1,3-Gal residues to the Man(9)GlcNAc(2) core structure...
  38. doi request reprint New insights into galactose metabolism by Schizosaccharomyces pombe: isolation and characterization of a galactose-assimilating mutant
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    J Biosci Bioeng 111:158-66. 2011
    ..We suggest that gal7+, gal10+, and gal1+ are localized close to a chromosomal terminal repressed by gene silencing in S. pombe. In contrast, gene silencing was defective in the FG2-8 strain making galactose assimilation possible...
  39. doi request reprint The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
    FEMS Yeast Res 13:259-66. 2013
    ..Taken together, these results suggest that nonsexual flocculation in fission yeast is negatively controlled by Gsf1, which controls expression of mbx2(+) and gsf2(+) ...
  40. doi request reprint Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka, Japan
    Appl Microbiol Biotechnol 95:441-9. 2012
    ..We suggest that S. pombe has a novel ethanol and/or 1-propanol recognition mechanism that upregulates glycerol utilization during glucose starvation...
  41. ncbi request reprint PhpA, a tyrosine phosphatase of Myxococcus xanthus, is involved in the production of exopolysaccharide
    Yumi Mori
    Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Kagawa 761 0795, Japan
    Microbiology 158:2546-55. 2012
    ..The EPS production assay showed that the phpA mutant produced an increased amount of EPS in comparison with the wild-type. These results indicate that PhpA may negatively regulate the production of EPS in M. xanthus...
  42. pmc Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
    Eukaryot Cell 11:159-67. 2012
    ..These results indicate that Ssp2 regulates phosphorylation and subcellular localization of Scr1 in response to glucose...
  43. pmc Protein O-mannosyltransferases B and C support hyphal development and differentiation in Aspergillus nidulans
    Masatoshi Goto
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6 10 1 Hakozaki, Fukuoka 812 8581, Japan
    Eukaryot Cell 8:1465-74. 2009
    ....
  44. ncbi request reprint A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Microbiology 152:2309-21. 2006
    ....
  45. ncbi request reprint A set of loxP marker cassettes for Cre-mediated multiple gene disruption in Schizosaccharomyces pombe
    Tomoko Iwaki
    Department of Life Sciences, Faculty of Agriculture, Kagawa University
    Biosci Biotechnol Biochem 68:545-50. 2004
    ..The use of the Cre-loxP system proved to be a practical strategy to excise a marker gene for repeated use in S. pombe...
  46. doi request reprint Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system
    Kaoru Takegawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    Biotechnol Appl Biochem 53:227-35. 2009
    ..This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains...
  47. ncbi request reprint Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe
    Yasuko Fujita
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Ikenobe, Japan
    Biosci Biotechnol Biochem 68:306-11. 2004
    ..pombe cells. We constructed a bacterial-S. pombe shuttle vector containing cys1a+ as a selective marker gene. The combination of the cysteine auxotroph and new vector could be useful for the expression of a heterologous protein...
  48. ncbi request reprint Identification of the fnx1+ and fnx2+ genes for vacuolar amino acid transporters in Schizosaccharomyces pombe
    Soracom Chardwiriyapreecha
    Faculty of Agriculture, Ehime University, Ehime 790 8566, Japan
    FEBS Lett 582:2225-30. 2008
    ..In both Deltafnx1 and Deltafnx2 mutant cells, uptake of lysine, isoleucine or asparagine was impaired. These results suggest that fnx1(+) and fnx2(+) are involved in vacuolar amino acid uptake in S. pombe...
  49. pmc A Myxococcus xanthus bacterial tyrosine kinase, BtkA, is required for the formation of mature spores
    Yoshio Kimura
    Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa, Japan
    J Bacteriol 193:5853-7. 2011
    ..The btkA mutant was unable to complete maturation to heat- and sonication-resistant spores under both starvation- and glycerol-induced developmental conditions...
  50. ncbi request reprint Homocysteine accumulation causes a defect in purine biosynthesis: further characterization of Schizosaccharomyces pombe methionine auxotrophs
    Yasuko Fujita
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761 0795, Japan
    Microbiology 152:397-404. 2006
    ..cerevisiae cystathionine pathway decreased total homocysteine levels. These data demonstrate that accumulation of homocysteine causes a defect in purine biosynthesis in the met26 mutant...
  51. doi request reprint Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast
    Shotaro Suzuki
    Department of Applied Biological Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Microbiology 156:708-18. 2010
    ..pombe...
  52. pmc MADS box transcription factor Mbx2/Pvg4 regulates invasive growth and flocculation by inducing gsf2+ expression in fission yeast
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Fukuoka, Japan
    Eukaryot Cell 11:151-8. 2012
    ..In addition, we found that fission yeast Mbx2 induces the nonsexual flocculation of budding yeast by the activation of FLO1...
  53. pmc Genome sequence of Pantoea agglomerans strain IG1
    Tomohiko Matsuzawa
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
    J Bacteriol 194:1258-9. 2012
    ..This genome sequence represents a substantial step toward the elucidation of pathways for production of lipopolysaccharides...
  54. doi request reprint Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3',5'- and 2',3'-cAMP phosphodiesterase, and phosphatase activities
    Yoshio Kimura
    Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa, Japan
    FEBS Lett 583:443-8. 2009
    ..The enzyme activities of PdeA and PdeB were stimulated by 50 microM Mn(2+) or Co(2+). The K(m) values of PdeA and PdeB for 3',5'-cAMP, 2',3'-cAMP, 5'-ATP, and 5'-AMP were in the low micromolar range (1.4-12.5 microM)...
  55. ncbi request reprint Identification of the catalytic acid base residue of arthrobacter endo-beta-N-acetylglucosaminidase by chemical rescue of an inactive mutant
    Kiyotaka Fujita
    Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima, Japan
    J Biochem 142:301-6. 2007
    ..This is the first identification of the catalytic residue for GH family 85 endo-beta-N-acetylglucosaminidases...
  56. pmc Enzymatic and functional analysis of a protein phosphatase, Pph3, from Myxococcus xanthus
    Yoshio Kimura
    Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa, Japan 761 0795
    J Bacteriol 193:2657-61. 2011
    ..The pph3 mutant under starvation conditions formed immature fruiting bodies and reduced sporulation...
  57. pmc Glycine betaine biosynthesized from glycine provides an osmolyte for cell growth and spore germination during osmotic stress in Myxococcus xanthus
    Yoshio Kimura
    Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa, Japan 761 0795
    J Bacteriol 192:1467-70. 2010
    ..Disruption of the M. xanthus gsmA (MXAN 7068) or sdmA (MXAN 3190) gene, encoding Gsm or Sdm homologue proteins, respectively, generated mutants that exhibited a longer lag period of growth and delayed spore germination under osmostress...
  58. doi request reprint A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling
    Kayo Hasehira
    Department of Functional Glycomics, Life Science Research Center, Kagawa University, 1750 1 Ikenobe, Miki cho, Kita gun, Kagawa, Japan
    J Biochem 147:501-9. 2010
    ..7 mg psicose/g leaf. The result proved feasibility of the method even for natural products. Because the system is a comprehensive tool, it should help answer questions concerning the biosyntheses and functions of rare hexose sugars...
  59. pmc An adenylyl cyclase, CyaA, of Myxococcus xanthus functions in signal transduction during osmotic stress
    Yoshio Kimura
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa, Japan 761 0795
    J Bacteriol 184:3578-85. 2002
    ..These results suggested that M. xanthus CyaA functions mainly as an ionic osmosensor during spore germination and that CyaA is also required for osmotic tolerance in fruiting formation and sporulation...
  60. pmc An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus
    Yoshio Kimura
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa, Japan 761 0795
    J Bacteriol 187:3593-8. 2005
    ..Under nonionic osmostress, the cyaB mutant exhibited reduced spore germination; however, the germination rate of the cyaB mutant was significantly higher than that of the cyaA mutant...
  61. ncbi request reprint A simple and efficient procedure for transformation of Schizosaccharomyces pombe
    Tomotake Morita
    Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    Yeast 21:613-7. 2004
    ..The method is simple and time-saving, making it especially useful for a large number of samples and when a high transformation efficiency is not required...
  62. pmc Genome sequence of the white koji mold Aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu
    Taiki Futagami
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan
    Eukaryot Cell 10:1586-7. 2011
    ..Analysis of the sequence may provide insight into the properties of this fungus that make it superior for use in shochu production, leading to the further development of A. kawachii for industrial applications...
  63. doi request reprint Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus
    Yoshio Kimura
    Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki cho, Kagawa 761 0795, Japan
    J Biosci Bioeng 107:10-5. 2009
    ..Phosphorylation of Kemptide was inhibited by a specific PKA inhibitor peptide, PKI(5-24), and the IC(50) and K(i) values for inhibition of the SpkA activity were 117 nM and 36 nM, respectively...
  64. ncbi request reprint Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "dehalococcoides" strains
    Taiki Futagami
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan
    Biosci Biotechnol Biochem 75:1268-74. 2011
    ..These results suggest that methanogens and sulfate-reducing bacteria can play a significant role in dechlorination by "Dehalococcoides."..
  65. ncbi request reprint Enzymatic synthesis of neoglycoconjugates by transglycosylation with endo-beta-N-acetylglucosaminidase A
    Kaoru Takegawa
    Department of Life Sciences, Kagawa University, Mikicho, Kita gun, Kagawa 761 0795, Japan
    Methods Enzymol 362:64-74. 2003
  66. doi request reprint Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses
    Shin ichi Nakakita
    Department of Functional Glycomics, Life Science Research Center, Kagawa University, 1750 1 Ikenobe, Miki cho, Kita gun, Kagawa 761 0793, Japan
    J Chromatogr A 1216:5112-5. 2009
    ..Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses...
  67. pmc A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast
    Kyotaro Hirashima
    ASPEX Division, Asahi Glass Co, Ltd Hazawa cho, Yokohama, Kanagawa 221 8755, Japan
    Nucleic Acids Res 34:e11. 2006
    ..For this reason, it is expected that the system will be applicable to various yeasts...
  68. ncbi request reprint Characterization of endo-beta-N-acetylglucosaminidase from alkaliphilic Bacillus halodurans C-125
    Kiyotaka Fujita
    Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
    Biosci Biotechnol Biochem 68:1059-66. 2004
    ..Although the C-terminal tail region is not essential for enzyme activity, the sequence is also conserved among endo-beta-N-acetylglucosaminidases of various origins...
  69. ncbi request reprint Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in Schizosaccharomyces pombe
    Masayuki Onishi
    Laboratory of Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Yeast 20:193-206. 2003
    ..pombe...
  70. pmc Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast
    Ayako Kita
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Mol Biol Cell 15:2920-31. 2004
    ....
  71. ncbi request reprint Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe
    Takako Koga
    Laboratory of Biological Chemistry, Graduate School of Agricultural and Life Science, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Genes Cells 9:561-74. 2004
    ..These results suggest that Vps5p and Vps17p are the targets of PtdIns(3)P in vesicle transport required for onset of the forespore membrane formation...
  72. pmc Role of the Rab GTP-binding protein Ypt3 in the fission yeast exocytic pathway and its connection to calcineurin function
    Hong Cheng
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Japan
    Mol Biol Cell 13:2963-76. 2002
    ..These results suggest that Ypt3 is required at multiple steps of the exocytic pathway and its mutation affects diverse cellular processes and that calcineurin is functionally connected to these cellular processes...
  73. pmc Schizosaccharomyces pombe Sst4p, a conserved Vps27/Hrs homolog, functions downstream of phosphatidylinositol 3-kinase Pik3p to mediate proper spore formation
    Masayuki Onishi
    Laboratory of Biological Chemistry, Graduate School of Agricultural and Life Science, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    Eukaryot Cell 6:2343-53. 2007
    ..These results suggest that Sst4p is a downstream factor of PtdIns 3-kinase and functions in forespore membrane formation...
  74. ncbi request reprint A role for fission yeast Rab GTPase Ypt7p in sporulation
    Jun Kashiwazaki
    Department of Biology, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    Cell Struct Funct 30:43-9. 2005
    ..Vacuoles in sporulating cells were found to undergo extensive homotypic vacuole fusion to form a few large compartments occupying the entire cytoplasm of asci. This extensive vacuole fusion depended on Ypt7p...
  75. ncbi request reprint Pmr1, a P-type ATPase, and Pdt1, an Nramp homologue, cooperatively regulate cell morphogenesis in fission yeast: the importance of Mn2+ homeostasis
    Takuya Maeda
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
    Genes Cells 9:71-82. 2004
    ..These results suggest that Pmr1 and Pdt1 cooperatively regulate cell morphogenesis through the control of Mn2+ homeostasis, and that calcineurin functions as a Mn2+ sensor as well as a Mn2+ homeostasis regulator...
  76. pmc Valproic acid affects membrane trafficking and cell-wall integrity in fission yeast
    Makoto Miyatake
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe, Hyogo 650 0017, Japan
    Genetics 175:1695-705. 2007
    ..Altogether, our results suggest that VPA affects membrane trafficking, which leads to the enhanced sensitivity to cell-wall damage in fission yeast...
  77. ncbi request reprint Isolation of suppressor mutants of phosphatidylinositol 3-phosphate 5-kinase deficient cells in Schizosaccharomyces pombe
    Masayuki Onishi
    Laboratory of Biological Chemistry, Graduate School of Agricultural and Life Science, University of Tokyo, 1 1 1 Yayoi, Tokyo 113 8657, Japan
    Biosci Biotechnol Biochem 67:1772-9. 2003
    ..Since many of these gene products are homologous to the proteins involved in vesicle transport, sterility caused by inactivation of ste12 may be due to a disordered vesicle transport system...
  78. ncbi request reprint Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants
    Md Muniruzzaman Goldar
    Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690 8504, Japan
    Biosci Biotechnol Biochem 69:1422-6. 2005
    ..cerevisiae. Thus S. pombe moc1/sds23 and S. cerevisiae SDS23 or SDS24 are interchangeable with each other, but their disruptants are phenotypically dissimilar...
  79. ncbi request reprint Genetic and functional interaction between Ryh1 and Ypt3: two Rab GTPases that function in S. pombe secretory pathway
    Yi He
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Genes Cells 11:207-21. 2006
    ..These results suggest that Ryh1 is involved in the secretory pathway and may have a potential overlapping function with Ypt3 in addition to its role in recycling...
  80. ncbi request reprint Schizosaccharomyces pombe minimum genome factory
    Yuko Giga-Hama
    ASPEX Division, Asahi Glass Co, Ltd, Hazawa cho, Yokohama, Kanagawa 221 8755, Japan
    Biotechnol Appl Biochem 46:147-55. 2007
    ..By preparing strains in which large chromosomal regions have been deleted and then combining strains defective in various functional genes, the establishment of effective hosts will become possible...
  81. ncbi request reprint Disruption of the plr1+ gene encoding pyridoxal reductase of Schizosaccharomyces pombe
    Tomotake Morita
    Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783 8502
    J Biochem 135:225-30. 2004
    ..The results showed that PL reductase encoded by the plr1(+ )gene is involved in the excretion of PL after reducing it to PN, and may not participate in the salvage pathway for PLP synthesis...
  82. ncbi request reprint Chemo-enzymatic synthesis of eel calcitonin glycosylated at two sites with the same and different carbohydrate structures
    Katsuji Haneda
    The Noguchi Institute, 1 8 1, Kaga, Itabashi, Tokyo 173 0003, Japan
    Carbohydr Res 341:181-90. 2006
    ..The effect appeared to be predictable from the results of mono-N-glycosylated calcitonin derivatives...
  83. ncbi request reprint Galactinol synthase gene of Coptis japonica is involved in berberine tolerance
    Kojiro Takanashi
    Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611 0011, Japan
    Biosci Biotechnol Biochem 72:398-405. 2008
    ..japonica cDNA library. One cDNA clone, which conferred clear berberine tolerance, encoded galactinol synthase (CjGolS). The possible role of CjGolS in berberine tolerance is discussed...
  84. ncbi request reprint Six new amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast
    Yan Ma
    Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo 650 0017, Japan
    Curr Genet 52:97-105. 2007
    ..17c (named cys2 (+)). The cys2 mutation required cysteine rather than methionine. These new tools, specifically, new selectable markers, will be useful in further genetic and biological studies in fission yeast...