Hiroshi Mizuno

Summary

Affiliation: Juntendo University School of Medicine
Country: Japan

Publications

  1. ncbi request reprint Adipose-derived stem cells: current findings and future perspectives
    Morikuni Tobita
    Department of Dentistry and Oral Surgery, Self Defense Force Hospital, Yokosuka, Japan
    Discov Med 11:160-70. 2011
  2. ncbi request reprint Adipose-derived stem and stromal cells for cell-based therapy: current status of preclinical studies and clinical trials
    Hiroshi Mizuno
    Juntendo University School of Medicine, Department of Plastic and Reconstructive Surgery, 2 1 1 Hongo Bunkyo ku, Tokyo 1138421, Japan
    Curr Opin Mol Ther 12:442-9. 2010
  3. doi request reprint Concise review: Adipose-derived stem cells as a novel tool for future regenerative medicine
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan
    Stem Cells 30:804-10. 2012
  4. ncbi request reprint Adipogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice-including relationship of sex differences
    Rei Ogawa
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, 1 1 5 Sendagi Bunkyo ku, Tokyo 113 8603, Japan
    Biochem Biophys Res Commun 319:511-7. 2004
  5. doi request reprint The effect of adipose-derived stem cells on ischemia-reperfusion injury: immunohistochemical and ultrastructural evaluation
    A Cagri Uysal
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Plast Reconstr Surg 124:804-15. 2009
  6. ncbi request reprint In vivo adipose tissue regeneration by adipose-derived stromal cells isolated from GFP transgenic mice
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Cells Tissues Organs 187:177-85. 2008
  7. doi request reprint Effect of mesenchymal stem cells on skin graft to flap prefabrication: an experimental study
    Cagri A Uysal
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Ann Plast Surg 65:237-44. 2010
  8. ncbi request reprint Chondrogenic and osteogenic differentiation of adipose-derived stem cells isolated from GFP transgenic mice
    Rei Ogawa
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    J Nippon Med Sch 71:240-1. 2004
  9. doi request reprint Comparison of readily available scaffolds for adipose tissue engineering using adipose-derived stem cells
    Yurie Itoi
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo 1138603, Japan
    J Plast Reconstr Aesthet Surg 63:858-64. 2010
  10. doi request reprint Periodontal tissue regeneration with adipose-derived stem cells
    Morikuni Tobita
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Tissue Eng Part A 14:945-53. 2008

Collaborators

Detail Information

Publications42

  1. ncbi request reprint Adipose-derived stem cells: current findings and future perspectives
    Morikuni Tobita
    Department of Dentistry and Oral Surgery, Self Defense Force Hospital, Yokosuka, Japan
    Discov Med 11:160-70. 2011
    ..This review will summarize data from in vitro studies, pre-clinical animal models, and recent clinical trials concerning the use of adipose-derived stem cells in regenerative medicine...
  2. ncbi request reprint Adipose-derived stem and stromal cells for cell-based therapy: current status of preclinical studies and clinical trials
    Hiroshi Mizuno
    Juntendo University School of Medicine, Department of Plastic and Reconstructive Surgery, 2 1 1 Hongo Bunkyo ku, Tokyo 1138421, Japan
    Curr Opin Mol Ther 12:442-9. 2010
    ..This review summarizes and discusses current preclinical and clinical data on the use of ASCs in regenerative medicine and discusses the future applications of such cell-based therapies...
  3. doi request reprint Concise review: Adipose-derived stem cells as a novel tool for future regenerative medicine
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan
    Stem Cells 30:804-10. 2012
    ..In addition, future directions for ASCs in terms of cell-based therapies and regenerative medicine are discussed...
  4. ncbi request reprint Adipogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice-including relationship of sex differences
    Rei Ogawa
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, 1 1 5 Sendagi Bunkyo ku, Tokyo 113 8603, Japan
    Biochem Biophys Res Commun 319:511-7. 2004
    ..89 times greater than those harvested from male mice. This suggests that the adipogenic differentiation of ASCs is closely related to sex differences...
  5. doi request reprint The effect of adipose-derived stem cells on ischemia-reperfusion injury: immunohistochemical and ultrastructural evaluation
    A Cagri Uysal
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Plast Reconstr Surg 124:804-15. 2009
    ....
  6. ncbi request reprint In vivo adipose tissue regeneration by adipose-derived stromal cells isolated from GFP transgenic mice
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Cells Tissues Organs 187:177-85. 2008
    ..ASCs may be an ideal source for adipose tissue regeneration, which may in turn play an important role in augmentation surgery in surgically treated cancer or trauma patients...
  7. doi request reprint Effect of mesenchymal stem cells on skin graft to flap prefabrication: an experimental study
    Cagri A Uysal
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Ann Plast Surg 65:237-44. 2010
    ..The Vascular Endothelial Growth Factor immunohistochemical staining was quantified. Furthermore, mesenchymal stem cells could be helpful in any prefabrication procedure in which neovascularization is indispensable...
  8. ncbi request reprint Chondrogenic and osteogenic differentiation of adipose-derived stem cells isolated from GFP transgenic mice
    Rei Ogawa
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    J Nippon Med Sch 71:240-1. 2004
    ..This cell population can be easily identified through fluorescence microscope, it should be an ideal source of ASCs for further experiments of stem cell biology and tissue engineering...
  9. doi request reprint Comparison of readily available scaffolds for adipose tissue engineering using adipose-derived stem cells
    Yurie Itoi
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo 1138603, Japan
    J Plast Reconstr Aesthet Surg 63:858-64. 2010
    ..PPAR-gamma2 gene expression in the induced ASCs in the type I collagen sponge was observed. These findings suggest that type I collagen sponge may be the most suitable among the three readily available scaffolds for adipogenesis...
  10. doi request reprint Periodontal tissue regeneration with adipose-derived stem cells
    Morikuni Tobita
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Tissue Eng Part A 14:945-53. 2008
    ..Because large amounts of human lipoaspirates are readily available, and their procurement induces only low morbidity, ASCs may be useful in future clinical cell-based therapy for periodontal disease...
  11. pmc Stimulatory Effect of Autologous Adipose Tissue-Derived Stromal Cells in an Atelocollagen Matrix on Wound Healing in Diabetic db/db Mice
    Masaki Nambu
    Department of Plastic and Reconstructive Surgery, National Defense Medical College, Saitama 359 8513, Japan
    J Tissue Eng 2011:158105. 2011
    ....
  12. doi request reprint Accelerated wound healing in healing-impaired db/db mice by autologous adipose tissue-derived stromal cells combined with atelocollagen matrix
    Masaki Nambu
    Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan
    Ann Plast Surg 62:317-21. 2009
    ..These results suggested that transplantation of autologous ATSCs-containing ACMS significantly accelerated wound healing in diabetic healing-impaired db/db mice...
  13. doi request reprint Differentiated and undifferentiated adipose-derived stem cells improve function in rats with peripheral nerve gaps
    Hakan Orbay
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    J Plast Reconstr Aesthet Surg 65:657-64. 2012
    ..05). These results reveal the therapeutic potential of adipose-derived stem cells in nerve reconstruction...
  14. doi request reprint Adipose-derived stem cells for periodontal tissue regeneration
    Morikuni Tobita
    Office of Surgeon General and Director of Medicine, Maritime Staff Office, Ministry of Defense, Tokyo, Japan
    Methods Mol Biol 702:461-70. 2011
    ..In this chapter, we introduce a rat periodontal tissue regeneration model using adipose-derived stem cells...
  15. doi request reprint Effects of adipose-derived stem cells on improving the viability of diced cartilage grafts
    Hakan Orbay
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Plast Reconstr Surg 129:369-77. 2012
    ..Diced cartilage grafts are useful for dorsal nasal augmentation; however, long-term resorption usually occurs. The aim of this study was to examine the effects of adipose-derived stem cells on the viability of diced cartilage grafts...
  16. doi request reprint Improved viability of random pattern skin flaps through the use of adipose-derived stem cells
    Feng Lu
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Plast Reconstr Surg 121:50-8. 2008
    ....
  17. doi request reprint Prefabrication of tissue engineered bone grafts: an experimental study
    Takahisa Okuda
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Ann Plast Surg 64:98-104. 2010
    ..5).The promising results obtained with prefabrication in tissue engineered bone grafts encourage the clinical application of this technology. Thus, prefabrication may be a useful technique in any engineered bone tissue transfer...
  18. doi request reprint Adipose-derived stem cells for skin regeneration
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, School of Medicine, Juntendo University, Tokyo, Japan
    Methods Mol Biol 702:453-9. 2011
    ..These findings suggest that ASCs may have a positive effect on wound healing and may be a useful tool for future cell-based therapy...
  19. ncbi request reprint Concentration of PDGF-AB, BB and TGF-β1 as valuable human serum parameters in adipose-derived stem cell proliferation
    Fonny Josh
    Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan
    J Nippon Med Sch 80:140-7. 2013
    ..The purpose of this study was to determine what types of HS, with respect to the concentrations of endogenous growth factors, could be made available for hASC proliferation...
  20. ncbi request reprint Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review
    Hiroshi Mizuno
    Department of Plastic, Reconstructive and Regenerative Surgery, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan
    J Nippon Med Sch 76:56-66. 2009
    ....
  21. doi request reprint Therapeutic angiogenesis by autologous bone marrow cell implantation together with allogeneic cultured dermal substitute for intractable ulcers in critical limb ischaemia
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Tokyo 113 8603, Japan
    J Plast Reconstr Aesthet Surg 63:1875-82. 2010
    ..Since the incidence of this condition might increase in the future, this therapeutic approach should play an important role in the preservation of ischaemic limbs...
  22. ncbi request reprint Neural differentiation of adipose-derived stem cells isolated from GFP transgenic mice
    Juri Fujimura
    Department of Pharmacology, Nippon Medical School, Tokyo, Japan
    Biochem Biophys Res Commun 333:116-21. 2005
    ....
  23. doi request reprint Acellular dermal matrix seeded with adipose-derived stem cells as a subcutaneous implant
    Hakan Orbay
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Tokyo, 113 8603, Japan
    Aesthetic Plast Surg 35:756-63. 2011
    ..Soft tissue augmentation using autologous materials usually is associated with high resorption rates. To obtain more durable soft tissue filler, acellular dermal matrix (ADM) was seeded with adipose-derived stem cells (ASCs) in this study...
  24. doi request reprint Clinical and vascular anatomical study of distally based sural flap
    Shimpo Aoki
    Departments of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Ann Plast Surg 61:73-8. 2008
    ..Subsequently, tissue specimens were harvested from the upper, middle, and lower sites of the flap for histologic analyses...
  25. doi request reprint Fat grafting to the breast and adipose-derived stem cells: recent scientific consensus and controversy
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, 1 1 5 Sendagi Bunkyo ku, Tokyo, Japan
    Aesthet Surg J 30:381-7. 2010
    ....
  26. doi request reprint Cylinder syringe suction: a simple and efficient technique for the evacuation of subcutaneous hematoma
    Ayato Hayashi
    Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan
    J Craniofac Surg 24:777-80. 2013
    ..The present paper introduces a simple technique of hematoma evacuation, called cylinder syringe suction (CSS). Experiments were also performed to determine the detailed mechanism underlying its effectiveness...
  27. ncbi request reprint Lower leg reconstruction using three free flaps
    Rei Ogawa
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Plast Reconstr Surg 120:2130-1. 2007
  28. ncbi request reprint The potential for treatment of skeletal muscle disorders with adipose-derived stem cells
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, 1 1 5 Sendagi Bunkyo ku, Tokyo 1138603, Japan
    Curr Stem Cell Res Ther 5:133-6. 2010
    ....
  29. doi request reprint Preclinical efficacy of slow-release bFGF in ischemia-reperfusion injury in a Dorsal Island skin flap model
    Yang Wang
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    J Reconstr Microsurg 29:341-6. 2013
    ..01). In conclusion, slow-release bFGF prevents ischemia-reperfusion injury by upregulating the secretion of vasculogenic growth factors...
  30. doi request reprint Adipose-derived stem cells enhance primary tendon repair: biomechanical and immunohistochemical evaluation
    Cagri A Uysal
    Department of Plastic and Reconstructive Surgery, Baskent University Faculty of Medicine, Ankara, Turkey
    J Plast Reconstr Aesthet Surg 65:1712-9. 2012
    ..In this study, we sought to determine whether ASCs would be beneficial in primary tendon healing...
  31. ncbi request reprint Enhanced healing of mitomycin C-treated wounds in rats using inbred adipose tissue-derived stromal cells within an atelocollagen matrix
    Masaki Nambu
    Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan
    Wound Repair Regen 15:505-10. 2007
    ..Thus, this study suggested that transplantation of inbred ATSC-containing ACMS is effective for repairing healing-impaired wounds...
  32. ncbi request reprint Frontal musculocutaneous V-Y island flap for coverage of forehead defect with a dural exposure after craniotomy
    Hiroshi Mizuno
    Department of Plastic, Reconstructive and Regenerative Surgery, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan
    J Nippon Med Sch 76:19-22. 2009
    ....
  33. ncbi request reprint Periodontal disease and periodontal tissue regeneration
    Morikuni Tobita
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Curr Stem Cell Res Ther 5:168-74. 2010
    ..In particular, adipose-derived stem cells are presented as an agent for restoring periodontal tissue defects...
  34. ncbi request reprint Accelerated and safe proliferation of human adipose-derived stem cells in medium supplemented with human serum
    Fonny Josh
    Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Hongo, Tokyo, Japan
    J Nippon Med Sch 79:444-52. 2012
    ..These results support the establishment of a safe and rapid expansion protocol with autologous serum for cell-based therapies, such as tissue engineering and regenerative medicine, using hASCs...
  35. ncbi request reprint Osteogenic and chondrogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice
    Rei Ogawa
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Toky 113 8603, Japan
    Biochem Biophys Res Commun 313:871-7. 2004
    ..Since this cell population can be easily identified through fluorescence microscopy, it may be an ideal source of ASCs for further experiments on stem cell biology and tissue engineering...
  36. pmc Mesenchymal stem cells isolated from adipose and other tissues: basic biological properties and clinical applications
    Hakan Orbay
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo 113 0022, Japan
    Stem Cells Int 2012:461718. 2012
    ..This paper summarizes data on the biological properties of MSCs and discusses current and potential clinical applications...
  37. pmc Prediction of limb salvage after therapeutic angiogenesis by autologous bone marrow cell implantation in patients with critical limb ischemia
    Shuhei Tara
    Division of Cardiology, Department of Internal Medicine, Nippon Medical School, Tokyo, Japan
    Ann Vasc Dis 4:24-31. 2011
    ..Conclusion: Maximum TcPO2 and (99m)Tc-TF perfusion index in the foot are promising predictors of limb salvage after BMCI in CLI...
  38. ncbi request reprint Adipose-derived stem cells and platelet-rich plasma: the keys to functional periodontal tissue engineering
    Morikuni Tobita
    Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 1138421, Japan
    Curr Stem Cell Res Ther 8:400-6. 2013
    ..In this review, the potential of adipose-derived stem cells and platelet-rich plasma is introduced. Of particular interest, the usefulness in periodontal tissue regeneration and future perspective is discussed. ..
  39. doi request reprint The medial plantar flap vascularized by the reverse flow lateral plantar artery: a novel variation through the case of aggressive digital papillary adenocarcinoma of the sole
    Ayato Hayashi
    Department of Plastic and Reconstructive Surgery, Juntendo University School of Medicine, Tokyo, Japan
    J Reconstr Microsurg 28:427-30. 2012
    ..However, the pedicle crossed the donor site somewhat tightly and the flap became congested for a while. Therefore, it is important to ensure careful handling of the donor site when performing this procedure...
  40. ncbi request reprint Simultaneous bilateral breast reconstruction with autologous tissue transfer after the removal of injectable artificial materials: a 12-year experience
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    Plast Reconstr Surg 116:450-8. 2005
    ..The purpose of this study was to review the clinical outcome of the authors' reconstruction procedures retrospectively...
  41. ncbi request reprint [Versatility of adipose tissue as a source of stem cells]
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Japan
    J Nippon Med Sch 70:428-31. 2003
    ..Since human adipose tissue is ubiquitous, easily obtainable in large quantity under local anesthesia with little patient discomfort, it may be an alternative stem cell source for mesenchymal tissue regeneration and engineering...
  42. ncbi request reprint Squamous cell carcinoma of the auricle arising from keloid after radium needle therapy
    Hiroshi Mizuno
    Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
    J Craniofac Surg 17:360-2. 2006
    ..Surgical interventions should always be the first choice in the treatment of not only the carcinomas but also in keloids in selected cases...