K Hiramatsu

Summary

Affiliation: Juntendo University School of Medicine
Country: Japan

Publications

  1. ncbi Vancomycin-resistant Staphylococcus aureus: a new model of antibiotic resistance
    K Hiramatsu
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Lancet Infect Dis 1:147-55. 2001
  2. ncbi The emergence and evolution of methicillin-resistant Staphylococcus aureus
    K Hiramatsu
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo Bunkyo ku, Tokyo 113 8421, Japan
    Trends Microbiol 9:486-93. 2001
  3. ncbi New trends in Staphylococcus aureus infections: glycopeptide resistance in hospital and methicillin resistance in the community
    Keiichi Hiramatsu
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Curr Opin Infect Dis 15:407-13. 2002
  4. ncbi Molecular genetics of methicillin-resistant Staphylococcus aureus
    Keiichi Hiramatsu
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    Int J Med Microbiol 292:67-74. 2002
  5. pmc Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community
    Keiko Okuma
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    J Clin Microbiol 40:4289-94. 2002
  6. ncbi Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin
    K Hiramatsu
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Lancet 350:1670-3. 1997
  7. ncbi Has vancomycin-resistant Staphylococcus aureus started going it alone?
    Keiichi Hiramatsu
    Department of Microbiology and Infection Control Science, Juntendo University Post Graduate School of Medicine, Tokyo 113 8421, Japan
    Lancet 364:565-6. 2004
  8. pmc Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus
    T Ito
    Department of Bacteriology, Juntendo University, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 45:1323-36. 2001
  9. ncbi DNA vaccination by mecA sequence evokes an antibacterial immune response against methicillin-resistant Staphylococcus aureus
    A Ohwada
    Department of Respiratory Medicine, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421 Japan
    J Antimicrob Chemother 44:767-74. 1999
  10. ncbi Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance
    Reiko Yoshida
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113, Japan
    J Antimicrob Chemother 51:247-55. 2003

Detail Information

Publications99

  1. ncbi Vancomycin-resistant Staphylococcus aureus: a new model of antibiotic resistance
    K Hiramatsu
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Lancet Infect Dis 1:147-55. 2001
    ..Vancomycin resistance is acquired by mutation and thickening of cell wall due to accumulation of excess amounts of peptidoglycan. This seems to be a common resistance mechanism for all VRSA strains isolated in the world so far...
  2. ncbi The emergence and evolution of methicillin-resistant Staphylococcus aureus
    K Hiramatsu
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo Bunkyo ku, Tokyo 113 8421, Japan
    Trends Microbiol 9:486-93. 2001
    ..aureus strains from the normal human flora into MRSA...
  3. ncbi New trends in Staphylococcus aureus infections: glycopeptide resistance in hospital and methicillin resistance in the community
    Keiichi Hiramatsu
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Curr Opin Infect Dis 15:407-13. 2002
    ..aureus evolution: the acquisition of multiple antibiotic resistance in the hospital and the trend towards methicillin-resistant S. aureus as a community pathogen...
  4. ncbi Molecular genetics of methicillin-resistant Staphylococcus aureus
    Keiichi Hiramatsu
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    Int J Med Microbiol 292:67-74. 2002
    ..Community-acquired MRSA (C-MRSA), may become a new threat to humans, and international cooperation of researchers and clinicians will be of cardinal importance in addressing this problem...
  5. pmc Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community
    Keiko Okuma
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    J Clin Microbiol 40:4289-94. 2002
    ..They multiplied much faster than health-care-associated MRSA and were resistant to fewer non-beta-lactam antibiotics. They seem to have been derived from more diverse S. aureus populations than health-care-associated MRSA strains...
  6. ncbi Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin
    K Hiramatsu
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Lancet 350:1670-3. 1997
    ..Two important questions need to be answered: (1) what is the prevalence of VRSA, and (2) by what mechanism does vancomycin resistance occur...
  7. ncbi Has vancomycin-resistant Staphylococcus aureus started going it alone?
    Keiichi Hiramatsu
    Department of Microbiology and Infection Control Science, Juntendo University Post Graduate School of Medicine, Tokyo 113 8421, Japan
    Lancet 364:565-6. 2004
  8. pmc Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus
    T Ito
    Department of Bacteriology, Juntendo University, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 45:1323-36. 2001
    ..Our findings indicated that there are at least three distinct MRSA clones in the world with different types of SCCmec in their chromosome...
  9. ncbi DNA vaccination by mecA sequence evokes an antibacterial immune response against methicillin-resistant Staphylococcus aureus
    A Ohwada
    Department of Respiratory Medicine, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421 Japan
    J Antimicrob Chemother 44:767-74. 1999
    ..Together these observations indicate that PBP2' or the mecA sequence may be eligible as a candidate molecule for vaccination against MRSA...
  10. ncbi Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance
    Reiko Yoshida
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113, Japan
    J Antimicrob Chemother 51:247-55. 2003
    ..The strain was characteristically a non-producer of autolytic enzyme, though the phenotype, which was lost easily, was not directly correlated with methicillin resistance...
  11. pmc Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains
    Xiao Xue Ma
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 46:1147-52. 2002
    ..Consistent with the strains' susceptibilities to various non-beta-lactam antibiotics, the type IV SCCmec was devoid of any antibiotic resistance genes other than the mecA gene...
  12. ncbi Molecular cloning and nucleotide sequence determination of the regulator region of mecA gene in methicillin-resistant Staphylococcus aureus (MRSA)
    K Hiramatsu
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    FEBS Lett 298:133-6. 1992
    ..These orfs are considered to encode putative co-inducer and repressor proteins specific for the regulation of methicillin resistance in MRSA...
  13. ncbi [MRSA (methicillin-resistant Staphylococcus aureus)]
    Teruyo Ito
    Department of Bacteriology, School of Medicine, Juntendo University
    Nihon Rinsho 61:164-70. 2003
  14. ncbi Genome and virulence determinants of high virulence community-acquired MRSA
    Tadashi Baba
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Lancet 359:1819-27. 2002
    ..By contrast with hospital-acquired MRSA, community-acquired MRSA is more susceptible to non b-lactam antibiotics. We investigated the high virulence potential of certain strains of this bacterium...
  15. pmc Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus
    Y Katayama
    Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 45:1955-63. 2001
    ..haemolyticus clinical strains and that the IS431-mediated mecI gene deletion causes the expression of methicillin resistance through the derepression of mecA gene transcription...
  16. ncbi Susceptibilities of healthcare- and community-associated methicillin-resistant staphylococci to the novel des-F(6)-quinolone DX-619
    Shinya Watanabe
    Department of Infection Control Science, Graduate School of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    J Antimicrob Chemother 60:1384-7. 2007
    ....
  17. pmc Methicillin-resistant Staphylococcus pseudintermedius in a veterinary teaching hospital
    Takashi Sasaki
    Department of Infection Control Science, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Clin Microbiol 45:1118-25. 2007
    ..To our knowledge, this is the first report of MRSP in dogs and humans in a veterinary institution...
  18. pmc Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions
    Yoko Kondo
    Department of Infection Control Science, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 51:264-74. 2007
    ..The SCCmec type assignments were identical to those made with a PCR system that uses numerous primer pairs to identify genes or gene alleles. Our system of six M-PCRs is thus a convenient and reliable method for typing SCCmec elements...
  19. doi Nationwide surveillance of bacterial respiratory pathogens conducted by the Japanese Society of Chemotherapy in 2007: general view of the pathogens' antibacterial susceptibility
    Y Niki
    The JSC Surveillance Committee, Japanese Society of Chemotherapy, Nichinai Kaikan B1, 3 28 8 Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    J Infect Chemother 15:156-67. 2009
    ..Continued surveillance is required to prevent the further spread of these antimicrobial resistances...
  20. pmc Combination effect of vancomycin and beta-lactams against a Staphylococcus aureus strain, Mu3, with heterogeneous resistance to vancomycin
    N Aritaka
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 45:1292-4. 2001
    ..This study implicated the unpredictable nature of combination therapy of beta-lactams and vancomycin against S. aureus with reduced susceptibility to vancomycin...
  21. pmc Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance
    Hui min Neoh
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan 113 8421
    Antimicrob Agents Chemother 52:45-53. 2008
    ..Interestingly, the overexpression of graR* increased the daptomycin MICs in both Mu3 and N315 and decreased the oxacillin MIC in N315...
  22. pmc Extreme genetic diversity of methicillin-resistant Staphylococcus epidermidis strains disseminated among healthy Japanese children
    Tengku Zetty Maztura Tengku Jamaluddin
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Clin Microbiol 46:3778-83. 2008
    ..Au contraire, with the case of CA-MRSE, there was no single genotype of CA-MRSE disseminated among children even in the same institution or class...
  23. pmc Staphylococcal cassette chromosome mec-like element in Macrococcus caseolyticus
    Sae Tsubakishita
    Department of Infection Control Science, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 54:1469-75. 2010
    ..However, its origin has been unknown. Our observation revealed a potential mechanism of the generation of a new SCCmec-like element in M. caseolyticus, a commensal bacterium of food animals...
  24. pmc Whole-genome sequencing of staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species
    Fumihiko Takeuchi
    Department of Microbiology and Infection Control Science, Juntendo University, Tokyo, Japan
    J Bacteriol 187:7292-308. 2005
    ..Such rearrangements could have brought genomic plasticity to this species and contributed to its acquisition of antibiotic resistance...
  25. ncbi Staphylococcal cassette chromosome mec(SCC mec) analysis of MRSA
    Teruyo Ito
    Department of Infection Control Science, Graduate School of Medicine, Juntendo University, Tokyo, Japan
    Methods Mol Biol 391:87-102. 2007
    ..In this chapter, we describe polymerase chain reaction methods to type SCCmec elements by first identifying the mec and ccr type, and subsequently identifying genes in the J regions...
  26. pmc Predominance of clones carrying Panton-Valentine leukocidin genes among methicillin-resistant Staphylococcus aureus strains isolated in Japanese hospitals from 1979 to 1985
    Xiao Xue Ma
    Juntendo University, Department of Bacteriology, Hongo 1 1 1 Bunkyo ku, Tokyo 113 8421, Japan
    J Clin Microbiol 44:4515-27. 2006
    ..The results also suggested that MRSA strains that predominated between 1979 and 1985 were generated from PVL-positive methicillin-susceptible S. aureus strains through the integration of SCCmec elements...
  27. pmc Reclassification of phenotypically identified staphylococcus intermedius strains
    Takashi Sasaki
    Department of Infection Control Science, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Clin Microbiol 45:2770-8. 2007
    ..delphini group A, S. delphini group B, and S. pseudintermedius were not found. In conclusion, SIG strains were reclassified into four clusters with three established and one probably novel species...
  28. ncbi Whole genome sequencing of meticillin-resistant Staphylococcus aureus
    M Kuroda
    Hiramatsu, Department of Bacteriology, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, 113-8421, Tokyo, Japan
    Lancet 357:1225-40. 2001
    ..Investigation of many newly identified gene products, including the 70 putative virulence factors, will greatly improve our understanding of the biology of staphylococci and the processes of infectious diseases caused by S aureus...
  29. ncbi Improvement of water-soluble cephalosporin derivatives having antibacterial activity against methicillin-resistant Staphylococcus aureus
    H Hanaki
    Department of Bacteriology, School of Medicine, Juntendo University, Tokyo, Japan
    Chemotherapy 47:170-6. 2001
    ..56, 12.5-25, 6.25, 1.56 and 1.56 microg/ml, respectively. These results indicated that the ammonioethyl and cyclic groups yield the maximum anti-MRSA and anti-Enterococcus faecalis activity, and also good water solubility...
  30. pmc Smqnr, a new chromosome-carried quinolone resistance gene in Stenotrophomonas maltophilia
    Kenichiro Shimizu
    Department of Infection Control Science, Faculty of Medicine, Juntendo University, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 52:3823-5. 2008
    ..The expression of Smqnr in Escherichia coli decreased the susceptibilities of the E. coli isolates to several fluoroquinolones...
  31. pmc Genetic diversity of staphylocoagulase genes (coa): insight into the evolution of variable chromosomal virulence factors in Staphylococcus aureus
    Shinya Watanabe
    Department of Infection Control Science, Graduate School of Medicine, Juntendo University, Bunkyo ku, Tokyo, Japan
    PLoS ONE 4:e5714. 2009
    ..Although SCs have been classified into 10 serotypes based on the differences in the antigenicity, genetic bases for their diversities and relatedness to chromosome types are poorly understood...
  32. pmc Dissemination of methicillin-resistant staphylococci among healthy Japanese children
    Ken Hisata
    Department of Bacteriology, School of Medicine, Juntendo University, Tokyo, Japan
    J Clin Microbiol 43:3364-72. 2005
    ..The fact that MRC-NS strains were prevalent in the community suggested that they might serve as a reservoir for the SCCmec element carried by MRSA strains disseminated in the community...
  33. ncbi Identification of the up- and down-regulated genes in vancomycin-resistant Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential hybridization method
    M Kuroda
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    Biochem Biophys Res Commun 269:485-90. 2000
    ..aureus. Presumed increased uptake of fructose and altered fatty acid metabolism may also contribute to vancomycin resistance by supplying more precursor metabolites for cell-wall synthesis...
  34. pmc Serial daptomycin selection generates daptomycin-nonsusceptible Staphylococcus aureus strains with a heterogeneous vancomycin-intermediate phenotype
    Ilana Lopes Baratella da Cunha Camargo
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 52:4289-99. 2008
    ..aureus and a transcription profile that partially overlapped that of heterogeneous vancomycin-intermediate S. aureus...
  35. ncbi [Community-associated methicillin-resistant Staphylococcus aureus: current status and molecular epidemiological perspective]
    Teruyo Ito
    Department of Bacteriology, Faculty of Medicine, Juntendo University
    Kansenshogaku Zasshi 78:459-69. 2004
    ..By using the SCCmec typing, strong evidence was provided for the independent derivation of healthcare-associated MRSA and C-MRSA clones...
  36. pmc Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315
    T Ito
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 43:1449-58. 1999
    ..However, there was no ORF that might encode mec DNA-specific transposase or integrase proteins, indicating that the mec DNA is not a transposon or a bacteriophage in nature...
  37. pmc Community-acquired methicillin-resistant Staphylococcus aureus, Uruguay
    Xiao Xue Ma
    Juntendo University, Tokyo, Japan
    Emerg Infect Dis 11:973-6. 2005
    ..The clone was distinct from the highly virulent community clone represented by strain MW2, although both clones carried Panton-Valentine leukocidin gene and cna gene...
  38. pmc Cell wall thickening is a common feature of vancomycin resistance in Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, Bunkyo ku, Tokyo 113 8421, Japan
    J Clin Microbiol 41:5-14. 2003
    ..aureus...
  39. ncbi Subinhibitory concentrations of beta-lactam induce haemolytic activity in Staphylococcus aureus through the SaeRS two-component system
    Hiroko Kuroda
    Department of Bacteriology, Juntendo University, Hongo, Tokyo, Japan
    FEMS Microbiol Lett 268:98-105. 2007
    ..These observations suggest that beta-lactam is able to induce the SaeRS system, which acts as a crucial signal transduction system for S. aureus pathogenicity rather than antimicrobial resistance...
  40. ncbi Augmentation of the bactericidal activities of human cathelicidin CAP18/LL-37-derived antimicrobial peptides by amino acid substitutions
    I Nagaoka
    Department of Host Defense and Biochemical Research, School of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    Inflamm Res 54:66-73. 2005
    ..In this study, to develop peptide derivatives with improved bactericidal actions, we utilized the amphipathic 18-mer peptide (K15-V32) of LL-37 as a template, and evaluated the activities of modified peptides...
  41. pmc Emergence of quinolone-resistant Bordetella pertussis in Japan
    Masayuki Ohtsuka
    Department of Infection Control Science, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    Antimicrob Agents Chemother 53:3147-9. 2009
    ..All of the NAL-resistant strains had the same D87G mutation in gyrA...
  42. pmc Postantibiotic effects and bactericidal activities of levofloxacin and gatifloxacin at concentrations simulating those of topical ophthalmic administration against fluoroquinolone-resistant and fluoroquinolone-sensitive methicillin-resistant Staphylococcu
    Saichi Hoshi
    Department of Infection Control Science, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 52:2970-3. 2008
    ..Levofloxacin and gatifloxacin at concentrations simulating those in eye drops showed lower bactericidal activities and shorter PAEs against fluoroquinolone-resistant strains than against fluoroquinolone-sensitive strains...
  43. ncbi Comparative analysis of MRSA
    Fumihiko Takeuchi
    Department of Infection Control Science, Juntendo University, Tokyo, Japan
    Methods Mol Biol 391:153-68. 2007
    ..We also introduce how to compare the pathways between strains that possess different sets of genes...
  44. pmc Selection of heterogeneous vancomycin-intermediate Staphylococcus aureus by imipenem
    Yuki Katayama
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    Antimicrob Agents Chemother 53:3190-6. 2009
    ..We conclude that exposure not only to VAN but also to beta-lactams may select for reduced glycopeptide susceptibility in S. aureus...
  45. ncbi Use of a sensitive chemiluminescence-based assay to evaluate the metabolic suppression activity of linezolid on methicillin-resistant Staphylococcus aureus showing reduced susceptibility to vancomycin
    Mitsutaka Komatsu
    Department of Pediatrics, Juntendo University, Hongo, Bunkyo ku, Japan
    J Microbiol Biotechnol 19:734-42. 2009
    ..The chemiluminescence assay facilitated sensitive and discriminative susceptibility testing within a relatively short time...
  46. pmc Correlation between Reduced Daptomycin Susceptibility and Vancomycin Resistance in Vancomycin-Intermediate Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan 113 8421
    Antimicrob Agents Chemother 50:1079-82. 2006
    ..This correlation is related to cell wall thickening, suggesting that, similar to the case with vancomycin resistance in VISA, the physical barrier of a thickened cell wall may contribute to daptomycin resistance in S. aureus...
  47. pmc Impact of reduced vancomycin susceptibility on the therapeutic outcome of MRSA bloodstream infections
    Hui min Neoh
    Infection Control Science, Faculty of Medicine, Juntendo University, Hongo 2 1 1, Bunkyo ku, Tokyo, 113 8421 Japan
    Ann Clin Microbiol Antimicrob 6:13. 2007
    ..The aim of this study was to determine whether clinical outcome of patients with methicillin-resistant Staphylococcus aureus (MRSA) bacteraemia was correlated with vancomycin susceptibility of the corresponding strains...
  48. pmc Contribution of vraSR and graSR point mutations to vancomycin resistance in vancomycin-intermediate Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo, Japan
    Antimicrob Agents Chemother 53:1231-4. 2009
    ..Using a combination of Mu50Omega whole-genome sequencing and genome engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50Omega...
  49. ncbi Rapid detection of Staphylococcus aureus strains having reduced susceptibility to vancomycin using a chemiluminescence-based drug-susceptibility test
    Yutaka Tajima
    Infection Control Sciences, Postgraduate School of Medicine, Juntendo University, Tokyo 113 8421, Japan
    J Microbiol Methods 70:434-41. 2007
    ..This rapid and reliable method appears to be promising for detection of vancomycin-intermediate S. aureus strains in clinical laboratories, and may supersede classical susceptibility testing...
  50. pmc Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, [corrected] reflecting the ancestral genome of the human-pathogenic staphylococci
    Tadashi Baba
    Department of Microbiology and Infection Control Science, Juntendo University, 2 1 1 Hongo, Bunkyo, Tokyo 113 8421, Japan
    J Bacteriol 191:1180-90. 2009
    ..aureus...
  51. pmc Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands
    Tadashi Baba
    Department of Microbiology and Infection Control Science, Juntendo University, Tokyo 113 8421, Japan
    J Bacteriol 190:300-10. 2008
    ..Prophages and pathogenicity islands play central roles in S. aureus virulence and evolution...
  52. pmc Multiplex-PCR method for species identification of coagulase-positive staphylococci
    Takashi Sasaki
    Department of Infection Control Science, Faculty of Medicine, Juntendo University, Tokyo 113 8421, Japan
    J Clin Microbiol 48:765-9. 2010
    ..delphini groups A and B. The present method was both sensitive (99.8%) and specific (100%). Our M-PCR assay will allow the routine species identification of CoPS isolates from various animal species for clinical veterinary diagnosis...
  53. pmc Two different Panton-Valentine leukocidin phage lineages predominate in Japan
    Xiao Xue Ma
    Department of Bacteriology, Juntendo University, Hongo 2 1 1, Bunkyo ku, Tokyo 113 8421, Japan
    J Clin Microbiol 46:3246-58. 2008
    ..aureus. That various SCCmec elements were carried by different strains of the same phage type suggests that S. aureus strains might independently acquire PVL phages before they acquire various SCCmec elements...
  54. ncbi Is Histoplasma capsulatum a native inhabitant of Japan?
    Ken Kikuchi
    Department of Infection Control Science, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Microbiol Immunol 52:455-9. 2008
    ..capsulatum by culture and Histoplasma-specific PCR in three independent laboratories. No H. capsulatum was detected by either method, therefore H. capsulatum is unlikely to be present in bat guano in Japanese caves...
  55. doi Recurrence of heterogeneous methicillin-resistant Staphylococcus aureus (MRSA) among the MRSA clinical isolates in a Japanese university hospital
    Kozue Kishii
    Department of Infection Control Science, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    J Antimicrob Chemother 62:324-8. 2008
    ..Since 2000, however, we started observing an increase in MRSA strains with low cefazolin MICs (MRCLSA). This study was performed to understand the phenomenon by characterization of the 'cefazolin-susceptible' MRSA strains...
  56. pmc Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements
    Piriyaporn Chongtrakool
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan 113 8421
    Antimicrob Agents Chemother 50:1001-12. 2006
    ..These data suggest that numerous MRSA clones are disseminated in Asian hospitals, and these consist of minor clones that are presumed to have arisen locally and major clones that are presumed to have been introduced from other countries...
  57. pmc Novel mechanism of antibiotic resistance originating in vancomycin-intermediate Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    Antimicrob Agents Chemother 50:428-38. 2006
    ....
  58. ncbi Two-component system VraSR positively modulates the regulation of cell-wall biosynthesis pathway in Staphylococcus aureus
    Makoto Kuroda
    Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Mol Microbiol 49:807-21. 2003
    ..The observation strongly indicates that VraSR constitutes a positive regulator of cell-wall peptidoglycan synthesis, and that is deeply involved in the expression of beta-lactam and glycopeptide resistance in S. aureus...
  59. pmc Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC
    Teruyo Ito
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    Antimicrob Agents Chemother 48:2637-51. 2004
    ..Unlike the extant SCCmec types, it carries a set of foreign genes encoding a restriction-modification system that might play a role in the stabilization of the element on the chromosome...
  60. pmc DNA microarray-based identification of genes associated with glycopeptide resistance in Staphylococcus aureus
    Longzhu Cui
    Department of Bacteriology, Faculty of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 49:3404-13. 2005
    ..We propose that the genes which raise glycopeptide resistance in S. aureus function toward altering the cell wall metabolic pathway...
  61. ncbi Genetic characterization of methicillin-resistant Staphylococcus aureus
    Keiichi Hiramatsu
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan 113 8421
    Vaccine 22:S5-8. 2004
    ..The latter domain constitutes the genetic information that makes S. aureus a notorious hospital pathogen...
  62. ncbi Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC
    Teruyo Ito
    Department of Bacteriology, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Drug Resist Updat 6:41-52. 2003
    ..aureus (MRSA) strains has provided us a bird's-eye view of the distribution of the mobile genetic elements in the bacterial chromosome that encode antibiotic resistance as well as pathogenicity in S. aureus...
  63. doi Investigation of nasal meticillin-resistant Staphylococcus aureus carriage in a haemodialysis clinic in Japan
    Y Uehara
    Department of Infection Control Science, Juntendo University, Faculty of Medicine, Bunkyo ku, Tokyo, Japan
    J Hosp Infect 84:81-4. 2013
    ..Infection control measures based on the US Centers for Disease Control and Prevention's recommendation showed limited spread of MRSA in a haemodialysis room...
  64. ncbi [Evaluation of antibiotics used for enterohemorrhagic Escherichia coli O157 enteritis--effect of various antibiotics on extracellular release of verotoxin]
    T Ito
    Department of Bacteriology, Faculty of Medicine, Juntendo University
    Kansenshogaku Zasshi 71:130-5. 1997
    ....
  65. pmc Identification of a novel variant of staphylococcal cassette chromosome mec, type II.5, and Its truncated form by insertion of putative conjugative transposon Tn6012
    Xiao Han
    Department of Infection Control Science, Graduate School of Medicine, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Antimicrob Agents Chemother 53:2616-9. 2009
    ..2 SCCmec of strain PL72 (previously isolated in Poland), and its J1 region variant caused by the deletion/insertion of putative conjugative transposon Tn6012, identified in four S. aureus genomes...
  66. pmc A novel gene, fudoh, in the SCCmec region suppresses the colony spreading ability and virulence of Staphylococcus aureus
    Chikara Kaito
    Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo ku, Tokyo, Japan
    PLoS ONE 3:e3921. 2008
    ..These findings suggest that fudoh in the SCCmec type-II region suppresses colony spreading and exotoxin production, and is involved in S. aureus pathogenesis...
  67. pmc Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus
    Yuki Katayama
    Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo, Japan
    J Bacteriol 185:2711-22. 2003
    ..The existence of an SCC without a mec determinant is indicative of a staphylococcal site-specific mobile genetic element that serves as a vehicle of transfer for various genetic markers between staphylococcal species...
  68. pmc Precise excision and self-integration of a composite transposon as a model for spontaneous large-scale chromosome inversion/deletion of the Staphylococcus haemolyticus clinical strain JCSC1435
    Shinya Watanabe
    Department of Infection Control Science, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    J Bacteriol 189:2921-5. 2007
    ..Identification and sequencing of the rearranged chromosomal loci revealed that ISSha1 of S. haemolyticus is responsible for the chromosome rearrangements...
  69. pmc Structural comparison of ten serotypes of staphylocoagulases in Staphylococcus aureus
    S Watanabe
    Department of Infection Control Science, Juntendo University, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan 113 8421
    J Bacteriol 187:3698-707. 2005
    ..These data indicate that coa could be transmitted to S. aureus, while the less homologous regions in coa presumed to be responsible for different antigenicities might have evolved independently...
  70. doi Staphylococcus aureus membrane and diacylated lipopeptide induce thymic stromal lymphopoietin in keratinocytes through the Toll-like receptor 2-Toll-like receptor 6 pathway
    Anh Tuan Vu
    Atopy Allergy Research Center, Juntendo University Graduate School of Medicine, Tokyo, Japan
    J Allergy Clin Immunol 126:985-93, 993.e1-3. 2010
    ....
  71. ncbi Antibacterial activity of dipropofol and related compounds
    Masahiro Ogata
    Kyoritsu University of Pharmacy, Tokyo, Japan
    Biol Pharm Bull 28:1120-2. 2005
    ..The results indicated that the dimeric structure of 2,6-diisopropylphenol moiety may play an important role in the antibacterial activity...
  72. ncbi Prospective study of antibiotic protocols for managing surgical site infections in children
    Satoko Ichikawa
    Department of Pediatric General and Urogenital Surgery, Juntendo University School of Medicine, Tokyo 113 8421, Japan
    J Pediatr Surg 42:1002-7; discussion 1007. 2007
    ..We adopted antibiotic (Ab) protocols for managing surgical site infections in children and assessed their effectiveness...
  73. doi The first nationwide surveillance of bacterial respiratory pathogens conducted by the Japanese Society of Chemotherapy. Part 1: a general view of antibacterial susceptibility
    Y Niki
    The JSC Surveillance Committee, Japan Japanese Society of Chemotherapy Nichinai Kaikan B1, 3 28 8 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Infect Chemother 14:279-90. 2008
    ..These data will be a useful reference for future periodic surveillance studies, as well as for investigations to control antimicrobial-resistant pathogens...
  74. ncbi Impaired suppression of processing in schizophrenic patients suggested by ERPs obtained in a selective attention task
    S Kamio
    Department of Psychiatry, Tokyo Metropolitan Matsuzawa Hospital, 2-1-1 Kamikitazawa, Setagaya-ku, 156-0057, Tokyo, Japan
    Schizophr Res 49:213-21. 2001
    ..Significant between-group differences were obtained specifically in the right ear attended condition, suggesting impaired suppression of processing in schizophrenics mediated in the left hemisphere...
  75. ncbi A point mutation in norA gene is responsible for quinolone resistance in Staphylococcus aureus
    Y Ohshita
    Department of Microbiology, Faculty of Medicine, Juntendo University, Tokyo, Japan
    Biochem Biophys Res Commun 172:1028-34. 1990
    ....
  76. ncbi Genome sequencing and annotation: an overview
    Makoto Kuroda
    Department of Bacteriology, Juntendo University, Tokyo, Japan
    Methods Mol Biol 266:29-45. 2004
    ..Function of genes is predicted by homology searches performed against either public or well-established protein databases. This chapter discusses each of these stages in a genome-sequencing project...
  77. ncbi Time-suppression test using a colorimetric probe (alamarBlue) that measures bacterial metabolic activity
    Mitsutaka Komatsu
    Department of Pediatrics, Juntendo University, Tokyo, Japan
    Biomed Res 29:171-3. 2008
    ..As an example, the effects of vancomycin, linezolid, and daptomycin on a representative strain of Staphylococcus aureus were estimated...
  78. doi Phenol-soluble modulin alpha 3 enhances the human neutrophil lysis mediated by Panton-Valentine leukocidin
    Isamu Hongo
    Department of Infection Control Science, Graduate School of Medicine, Juntendo University, Tokyo, Japan
    J Infect Dis 200:715-23. 2009
    ..Phenol-soluble modulin alpha 3 did, however, enhance PVL-mediated lysis of human PMNs...
  79. ncbi Prophages of Staphylococcus aureus Newman and their contribution to virulence
    Taeok Bae
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 62:1035-47. 2006
    ..S. aureus Newman lacking all four prophages was unable to cause disease, thereby revealing essential contributions of prophages to the pathogenesis of staphylococcal infections...
  80. ncbi Genetic differentiation of methicillin-resistant Staphylococcus aureus strains from Korea and Japan
    Kwan Soo Ko
    Asian Pacific Research Foundation for Infectious Diseases ARFID, Seoul, Korea
    Microb Drug Resist 11:279-86. 2005
    ..A further comprehensive study should be performed to document the hypotheses from this study...
  81. ncbi Two heterogeneously vancomycin-intermediate clinical isolates of methicillin-sensitive and methicillin-resistant Staphylococcus aureus in a Turkish university hospital: brief report of a surveillance study
    Muzeyyen Mamal Torun
    Int J Antimicrob Agents 26:508-10. 2005
  82. ncbi [beta-lactamase-producing activity and antimicrobial susceptibility of major pathogenic bacteria isolated from clinical samples. Japan beta-lactamase Research Group]
    Toyoko Oguri
    Juntendo University
    Jpn J Antibiot 55:1-28. 2002
    ..Sulbactam/cefoperazone, cefepime, and imipenem still have excellent antimicrobial activity. Rates of resistance to each antimicrobial agent differed more among institutions than among geographical regions...
  83. ncbi A novel methicillin-resistance cassette in community-acquired methicillin-resistant Staphylococcus aureus isolates of diverse genetic backgrounds
    Robert S Daum
    Department of Pediatrics, University of Chicago, Chicago, Illinois 60637, USA
    J Infect Dis 186:1344-7. 2002
    ..SCCmec type IV is present on the genome of CA-MRSA isolates. Its relatively small size and presence in isolates of diverse genetic backgrounds suggest that it may spread among S. aureus isolates...
  84. ncbi [Nucleic acid-based testing for methicillin-resistant Staphylococcus aureus infection]
    Teruyo Ito
    Department of Infection Control Science, Graduate School of Medicine, Juntendo University
    Nihon Rinsho 63:378-86. 2005
  85. pmc Nosocomial spread of a Staphylococcus capitis strain with heteroresistance to vancomycin in a neonatal intensive care unit
    Wil C Van Der Zwet
    Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands
    J Clin Microbiol 40:2520-5. 2002
    ..We conclude that heteroresistance to vancomycin occurs in S. capitis and might be the cause of therapeutic failures in NICUs. Moreover, heteroresistant strains can become endemic in such units...
  86. ncbi Nucleotide substitutions in Staphylococcus aureus strains, Mu50, Mu3, and N315
    Toshiko Ohta
    Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennohdai, Tukuba, Ibaraki 305 8575, Japan
    DNA Res 11:51-6. 2004
    ..All of the diverse genes and the high quality sequence of Mu50 can be viewed at the web site (http://133.5.48.239/VRSA/)...
  87. ncbi [Staphylococcus]
    Ken Hisata
    Department of Bacteriology, Juntendo University School of Medicine
    Nihon Rinsho 61:659-64. 2003
  88. ncbi Susceptibilities to antiseptic agents and distribution of antiseptic-resistance genes qacA/B and smr of methicillin-resistant Staphylococcus aureus isolated in Asia during 1998 and 1999
    Norihisa Noguchi
    Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192 0392, Japan
    J Med Microbiol 54:557-65. 2005
    ....
  89. ncbi Heterogeneous vancomycin-resistant Staphylococcus aureus (hetero-VISA) in Singapore
    Li Hwei Sng
    Int J Antimicrob Agents 25:177-9. 2005
  90. ncbi Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage phi MR11
    Mohammad Rashel
    Department of Biochemistry, Kochi Medical School, Nankoku 783 8505, Japan
    J Infect Dis 196:1237-47. 2007
    ..These results indicate that MV-L might be useful as a powerful therapeutic agent against multidrug-resistant S. aureus infections...
  91. pmc Emergence in Asian countries of Staphylococcus aureus with reduced susceptibility to vancomycin
    Jae Hoon Song
    Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University, Seoul, South Korea
    Antimicrob Agents Chemother 48:4926-8. 2004
    ..However, neither vancomycin-intermediate S. aureus nor vancomycin-resistant S. aureus isolates were found among MRSA isolates from Asian countries in this survey...
  92. pmc Vancomycin heteroresistance in methicillin-resistant Staphylococcus aureus, Taiwan
    Jiun Ling Wang
    Emerg Infect Dis 10:1702-4. 2004
  93. ncbi Development and validation of microarray-based assay for epidemiological study of MRSA
    Junnosuke Otsuka
    Probing Technology Laboratory, RIKEN, Wako, Saitama, Japan
    Mol Cell Probes 22:1-13. 2008
    ..Our data showed the usefulness of the oligonucleotide microarray based assay in identifying important marker sets, such as toxin genes, resistance genes, SCCmec elements, and coagulase genes, for the molecular epidemiology of S. aureus...
  94. ncbi Antibacterial activity of 2,4-diacetylphloroglucinol produced by Pseudomonas sp. AMSN isolated from a marine alga, against vancomycin-resistant Staphylococcus aureus
    Alim Isnansetyo
    Marine and Highland Bioscience Center, Saga University, 152 1 Shonan cho, Karatsu, Saga 847 0021, Japan
    Int J Antimicrob Agents 22:545-7. 2003
    ..The activity of DAPG did not directly correlate with levels of vancomycin resistance in VRSA and VRE. These results suggest that DAPG might be useful in developing new antibiotics against VRSA...
  95. ncbi [Evolution and spread of SCCmec among staphylococci]
    Teruyo Ito
    Department of Bacteriology, Juntendo University School of Medicine
    Nihon Rinsho 65:415-22. 2007
  96. ncbi [Staphylococcal infections]
    Keiko Okuma
    Department of Bacteriology, Juntendo University School of Medicine
    Nihon Rinsho 61:311-6. 2003
  97. ncbi Emergence of vancomycin resistance during therapy against methicillin-resistant Staphylococcus aureus in a burn patient--importance of low-level resistance to vancomycin
    Isao Haraga
    Department of Microbiology, School of Medicine, Fukuoka University, Nanakuma, Jonan ku, Fukuoka, Japan
    Int J Infect Dis 6:302-8. 2002
    ..aureus (VRSA) is most important. However, VLSA is difficult to detect by standard laboratory methods. We describe here improved methods to detect VLSA...
  98. pmc Enumeration of Legionella CFU by colony hybridization using specific DNA probes
    Wakako Satoh
    Technology and Development Laboratory, Kurita Water Industries Ltd, Kanagawa 243 0124, Japan
    Appl Environ Microbiol 68:6466-70. 2002
    ..The CH counts indicated almost the same results as CFU counts in cultivated samples derived from the water. It was concluded that it is possible to substitute the CH procedure for the conventional one...
  99. ncbi Standard nomenclature for the superantigens expressed by Staphylococcus
    Gerard Lina
    Centre Nationale de Reference des Staphylocoques, INSERM E0230, IFR Laennec, Lyon, France
    J Infect Dis 189:2334-6. 2004
    ....