Toshifumi Ohkusa

Summary

Affiliation: Jikei University School of Medicine
Country: Japan

Publications

  1. doi request reprint Newly developed antibiotic combination therapy for ulcerative colitis: a double-blind placebo-controlled multicenter trial
    Toshifumi Ohkusa
    Department of Gastroenterology and Hepatology, Jikei University, Tokyo, Japan
    Am J Gastroenterol 105:1820-9. 2010
  2. pmc Commensal bacteria can enter colonic epithelial cells and induce proinflammatory cytokine secretion: a possible pathogenic mechanism of ulcerative colitis
    Toshifumi Ohkusa
    Department of Gastroenterology and Hepatology, The Jikei University School of Medicine, Kashiwa, Chiba 277 8567, Japan
    J Med Microbiol 58:535-45. 2009
  3. ncbi request reprint Effectiveness of antibiotic combination therapy in patients with active ulcerative colitis: a randomized, controlled pilot trial with long-term follow-up
    Toshifumi Ohkusa
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Scand J Gastroenterol 40:1334-42. 2005
  4. pmc Combined TLR2/4-activated dendritic/tumor cell fusions induce augmented cytotoxic T lymphocytes
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    PLoS ONE 8:e59280. 2013
  5. pmc Regulation of tumor immunity by tumor/dendritic cell fusions
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    Clin Dev Immunol 2010:516768. 2010
  6. doi request reprint Comparison of several activity indices for the evaluation of endoscopic activity in UC: inter- and intraobserver consistency
    Taro Osada
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Inflamm Bowel Dis 16:192-7. 2010
  7. doi request reprint Colonic mucosal lesions associated with low-dose aspirin: a case control study
    Tomoyoshi Shibuya
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Scand J Gastroenterol 46:810-7. 2011
  8. doi request reprint Efficacy of antibiotic combination therapy in patients with active ulcerative colitis, including refractory or steroid-dependent cases
    Toshiki Uehara
    Department of Gastroenterology and Hepatology, Nihon University School of Medicine, Tokyo, Japan
    J Gastroenterol Hepatol 25:S62-6. 2010
  9. doi request reprint Correlations among total colonoscopic findings, clinical symptoms, and laboratory markers in ulcerative colitis
    Taro Osada
    Department of Gastroenterology, Jutendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    J Gastroenterol Hepatol 23:S262-7. 2008
  10. pmc Immunologic monitoring of cellular responses by dendritic/tumor cell fusion vaccines
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    J Biomed Biotechnol 2011:910836. 2011

Detail Information

Publications45

  1. doi request reprint Newly developed antibiotic combination therapy for ulcerative colitis: a double-blind placebo-controlled multicenter trial
    Toshifumi Ohkusa
    Department of Gastroenterology and Hepatology, Jikei University, Tokyo, Japan
    Am J Gastroenterol 105:1820-9. 2010
    ..Fusobacterium varium may contribute to ulcerative colitis (UC). We conducted a double-blind placebo-controlled multicenter trial to determine whether antibiotic combination therapy induces and/or maintains remission of active UC...
  2. pmc Commensal bacteria can enter colonic epithelial cells and induce proinflammatory cytokine secretion: a possible pathogenic mechanism of ulcerative colitis
    Toshifumi Ohkusa
    Department of Gastroenterology and Hepatology, The Jikei University School of Medicine, Kashiwa, Chiba 277 8567, Japan
    J Med Microbiol 58:535-45. 2009
    ..Certain commensal bacteria can invade colonic epithelial cells, activating early intracellular signalling systems to trigger host inflammatory reactions...
  3. ncbi request reprint Effectiveness of antibiotic combination therapy in patients with active ulcerative colitis: a randomized, controlled pilot trial with long-term follow-up
    Toshifumi Ohkusa
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Scand J Gastroenterol 40:1334-42. 2005
    ..Our goal was to assess whether an antibiotic combination therapy against F. varium is effective for induction and maintenance of remission of UC...
  4. pmc Combined TLR2/4-activated dendritic/tumor cell fusions induce augmented cytotoxic T lymphocytes
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    PLoS ONE 8:e59280. 2013
    ..Incorporating combined TLRs-activation and TGF-β1-blockade of DC/tumor may enhance the effectiveness of DC/tumor-based cancer vaccines and have the potential applicability to the field of adoptive immunotherapy...
  5. pmc Regulation of tumor immunity by tumor/dendritic cell fusions
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    Clin Dev Immunol 2010:516768. 2010
    ..This paper reviews tumor immune escape and current strategies employed in the field of tumor/DC fusions vaccine aimed at enhancing activation of TAAs-specific cytotoxic T cells in tumor microenvironment...
  6. doi request reprint Comparison of several activity indices for the evaluation of endoscopic activity in UC: inter- and intraobserver consistency
    Taro Osada
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Inflamm Bowel Dis 16:192-7. 2010
    ..This study evaluated inter- and intraobserver agreement in the assessment of ulcerative colitis (UC) activity using 4 established indices and a newly designed Modified 6-point Activity Index...
  7. doi request reprint Colonic mucosal lesions associated with low-dose aspirin: a case control study
    Tomoyoshi Shibuya
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Scand J Gastroenterol 46:810-7. 2011
    ..Recently, there has been a substantial increase in prescriptions for LDA. We analyzed the risk of colonic mucosal lesions associated with the long-term use of LDA...
  8. doi request reprint Efficacy of antibiotic combination therapy in patients with active ulcerative colitis, including refractory or steroid-dependent cases
    Toshiki Uehara
    Department of Gastroenterology and Hepatology, Nihon University School of Medicine, Tokyo, Japan
    J Gastroenterol Hepatol 25:S62-6. 2010
    ..Herein, we assessed whether antibiotic combination therapy is effective for active UC, including cases with steroid refractory or dependent disease...
  9. doi request reprint Correlations among total colonoscopic findings, clinical symptoms, and laboratory markers in ulcerative colitis
    Taro Osada
    Department of Gastroenterology, Jutendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    J Gastroenterol Hepatol 23:S262-7. 2008
    ..In the present study we assessed the relationship between endoscopic and histological findings, clinical symptoms, and laboratory data...
  10. pmc Immunologic monitoring of cellular responses by dendritic/tumor cell fusion vaccines
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    J Biomed Biotechnol 2011:910836. 2011
    ..In this review, we will discuss immunologic monitoring of cellular responses for the evaluation of cancer vaccines including fusions of DC and whole tumor cell...
  11. ncbi request reprint Nested culture method improves detection of Fusobacterium from stool in patients with ulcerative colitis
    Toyokazu Yukawa
    Department of Gastroenterology and Hepatology, The Jikei University Kashiwa Hospital, Kashiwa 277 8567, Chiba, Japan
    Jpn J Infect Dis 66:109-14. 2013
    ..This study suggests that the recovery of F. varium is related to UC, which aligns with results from previous studies that used mucosal culture, immunostaining, real-time PCR, and serological studies...
  12. doi request reprint Dendritic/pancreatic carcinoma fusions for clinical use: Comparative functional analysis of healthy- versus patient-derived fusions
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    Clin Immunol 135:384-400. 2010
    ..However, fusions from patients with metastatic pancreatic cancer induced increased expression levels of TGF-beta1 in CD4+ CD25high T cells and low levels of CTLs with decreased IFN-gamma production...
  13. ncbi request reprint Increased esophageal sensitivity to acid and saline in patients with nonerosive gastro-esophageal reflux disease
    Akihito Nagahara
    Department of Gastroenterology, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, Japan
    J Clin Gastroenterol 40:891-5. 2006
    ..To investigate the features of nonerosive reflux disease (NERD)...
  14. pmc Current immunotherapeutic approaches in pancreatic cancer
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    Clin Dev Immunol 2011:267539. 2011
    ..We offer our perspective on how to increase the clinical efficacy of immunotherapies for pancreatic cancer...
  15. ncbi request reprint Endoscopic, histological and serologic findings of gastric hyperplastic polyps after eradication of Helicobacter pylori: comparison between responder and non-responder cases
    Toshifumi Ohkusa
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Digestion 68:57-62. 2003
    ..We have compared endoscopic and serologic findings of responder and non-responder cases with hyperplastic polyps to try to identify the cause(s), other than H. pylori infection, of the formation or growth of gastric hyperplastic polyps...
  16. doi request reprint Colonic mucosal lesions associated with long-term or short-term administration of nonsteroidal anti-inflammatory drugs
    Tomoyoshi Shibuya
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Colorectal Dis 12:1113-21. 2010
    ..We assessed the risk of developing colonic mucosal lesions according to the duration of exposure to NSAIDs: short-term and/or long-term use...
  17. doi request reprint Cancer immunotherapy by fusions of dendritic cells and tumor cells
    Shigeo Koido
    Department of Internal Medicine, The Jikei University, Tokyo, Japan
    Immunotherapy 1:49-62. 2009
    ..This review deals with recent progress in the immunotherapy of cancer with fusions of DCs and tumor cells...
  18. pmc Augmentation of antitumor immunity by fusions of ethanol-treated tumor cells and dendritic cells stimulated via dual TLRs through TGF-β1 blockade and IL-12p70 production
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    PLoS ONE 8:e63498. 2013
    ..The alternative system is simple and may provide a platform for adoptive immunotherapy...
  19. pmc Antigen-specific polyclonal cytotoxic T lymphocytes induced by fusions of dendritic cells and tumor cells
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105 8461, Japan
    J Biomed Biotechnol 2010:752381. 2010
    ..We will discuss perspectives for future development of DCs/tumor fusions for CTL induction...
  20. pmc Seasonal variations in the onset of ulcerative colitis in Japan
    Shigeo Koido
    Shigeo Koido, Toshifumi Ohkusa, Kan Uchiyama, Hiroshi Arakawa, Hisao Tajiri, Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Chiba 277 8567, Japan
    World J Gastroenterol 19:9063-8. 2013
    ..To investigate seasonal variations in the onset and relapse of ulcerative colitis (UC) in Japanese patients...
  21. ncbi request reprint Double-balloon endoscopy for treatment of long-term abdominal discomfort due to small bowel penetration by an eel bone
    Tomoyoshi Shibuya
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Med Sci Monit 14:CS107-9. 2008
    ..Penetration of the GI tract by a fish bone was treated by laparotomy. DBE is a useful procedure for removal of a foreign body from the small bowel...
  22. ncbi request reprint Obstructing small bowel bezoars due to an agar diet: diagnosis using double balloon enteroscopy
    Taro Osada
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Intern Med 47:617-20. 2008
    ..The bezoars were elastic hard, crystallized objects. These bezoars were considered to have formed from highly-concentrated, dissolvable agar...
  23. pmc Clinical significance of serum procalcitonin in patients with ulcerative colitis
    Shigeo Koido
    Shigeo Koido, Toshifumi Ohkusa, Kazuki Takakura, Shunichi Odahara, Shintaro Tsukinaga, Toyokazu Yukawa, Jimi Mitobe, Mikio Kajihara, Kan Uchiyama, Hiroshi Arakawa, Hisao Tajiri, Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Chiba 277 8567, Japan
    World J Gastroenterol 19:8335-41. 2013
    ..To investigate the association of procalcitonin (PCT) with ulcerative colitis (UC) activity...
  24. doi request reprint The fatty acid profile of the erythrocyte membrane in initial-onset inflammatory bowel disease patients
    Kan Uchiyama
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University Kashiwa Hospital, 163 1 Kashiwa shita, Kashiwa, Chiba, 277 0004, Japan
    Dig Dis Sci 58:1235-43. 2013
    ..Dietary fatty acid intake influences the fatty acid profiles of vital cell membranes, which might be a source of inflammatory mediators...
  25. ncbi request reprint Overlap/switch to adefovir monotherapy for lamivudine-resistant patients who responded to combination therapy: a pilot controlled study
    Mashu Aizawa
    Department of Gastroenterology and Hepatology, Kashiwa Hospital, Kashiwa, Japan
    Intern Med 49:1067-72. 2010
    ..The aim of this study was to investigate the outcome of overlap/switch to adefovir dipivoxil (ADV) monotherapy for chronic hepatitis B (CHB) patients with lamivudine (LAM)-resistant HBV, who responded to LAM plus ADV combination therapy...
  26. ncbi request reprint Altered localization and expression of tight-junction proteins in a rat model with chronic acid reflux esophagitis
    Daisuke Asaoka
    Department of Gastroenterology, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo, 113 8421, Japan
    J Gastroenterol 40:781-90. 2005
    ..The aim of this study was to investigate the distribution and expression pattern of tight-junction proteins in the esophageal mucosa of control rats and rats with reflux esophagitis...
  27. ncbi request reprint Helicobacter pylori is a fragile bacteria when stored at low and ultra-low temperatures
    Toshifumi Ohkusa
    Department of Gastroenterology, Juntendo University School of Medicine, Division of Animal Center, Tokyo Medical and Dental University, Tokyo, Japan
    J Gastroenterol Hepatol 19:200-4. 2004
    ..A study was done on seven H. pylori strains and other intestinal bacteria to compare different temperatures for storage of organisms in saline solution...
  28. ncbi request reprint [Diagnosis of Helicobacter pylori infection: comparison with gold standard]
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Kashiwa Hospital, Kashiwa 277 8567, Japan
    Rinsho Byori 56:1007-13. 2008
    ..pylori infection. This review provides a general overview of the diagnostic methods and tests the characteristics (sensitivity and specificity) for H. pylori infection...
  29. ncbi request reprint Location of early colorectal cancers at fold-top may reduce the risk of lymph node metastasis
    Wensheng Pan
    Department of Gastroenterology, Juntendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    Dis Colon Rectum 49:579-87. 2006
    ..This study was designed to look for significant correlations between location of early colorectal cancer, distance from muscularis mucosae to muscularis propria, and the frequency of lymph node metastasis...
  30. ncbi request reprint Highly active antiretroviral therapy improved persistent lamivudine-resistant viremia in acute hepatitis B virus genotype Ae infection with coinfection of human immunodeficiency virus
    Mashu Aizawa
    Department of Gastroenterology and Hepatology, Kashiwa Hospital, Tokyo, Japan
    Hepatol Res 40:229-35. 2010
    ....
  31. ncbi request reprint [A long-term survival case of liver epithelioid hemangioendothelioma with multiple lung metastases that regressed by long-term administration of interleukin-2]
    Kei Otani
    Department of Emergency Medicine, The Jikei University School of Medicine, Japan
    Nihon Shokakibyo Gakkai Zasshi 109:2097-102. 2012
    ..This case suggests that rIL-2 may become a new therapy for EHE. We think that the report of the long-term survival of a case of EHE in which rIL-2 treatment was effective is extremely valuable...
  32. ncbi request reprint Fusobacterium varium localized in the colonic mucosa of patients with ulcerative colitis stimulates species-specific antibody
    Toshifumi Ohkusa
    Department of Gastroenterology, Juntendo University, School of Medicine, Tokyo, Japan
    J Gastroenterol Hepatol 17:849-53. 2002
    ..We have isolated bacteria from inflamed colonic mucosa, examined the localization of the species in the mucosa, and assayed for serum antibodies to the bacteria...
  33. doi request reprint Identification of bacteria from blood in febrile patients with ulcerative colitis by terminal restriction fragment length polymorphism profile analysis of 16S rRNA gene
    Akira Harada
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Scand J Gastroenterol 43:423-30. 2008
    ..The aim of this study was to detect bacteremia in febrile patients with ulcerative colitis (UC) using a new molecular approach...
  34. doi request reprint Peroxisome proliferator-activated receptor γ 2 mutation may cause a subset of ulcerative colitis
    Yo Aoyagi
    Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan
    Pediatr Int 52:729-34. 2010
    ..We attempted to demonstrate whether the functional imbalance between TLRs and PPARγ could lead to the onset and some polymorphisms of those genes could contribute to susceptibility to IBD...
  35. doi request reprint N-3 polyunsaturated fatty acid diet therapy for patients with inflammatory bowel disease
    Kan Uchiyama
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, Jikei University School of Medicine Kashiwa Hospital, Chiba, Japan
    Inflamm Bowel Dis 16:1696-707. 2010
    ..We investigated the influence of diet therapy involving the use of an "n-3 PUFA food exchange table" (n-3DP) on the fatty acid composition of the erythrocyte membranes of IBD patients and its remission-maintaining effects...
  36. pmc Cancer vaccine by fusions of dendritic and cancer cells
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 277 8567, Japan shigeo
    Clin Dev Immunol 2009:657369. 2009
    ..This review summarizes recent advances in concepts and techniques that are providing new impulses to DCs/tumor fusions-based cancer vaccination...
  37. ncbi request reprint Recurrent peptic ulcers in patients following successful Helicobacter pylori eradication: a multicenter study of 4940 patients
    Hiroto Miwa
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Helicobacter 9:9-16. 2004
    ..The aim of this study is to describe the recurrence rate and specific features of peptic ulcers after cure of H. pylori infection...
  38. ncbi request reprint Improvement of reflux symptoms 3 years after cure of Helicobacter pylori infection: a case-controlled study in the Japanese population
    Hiroto Miwa
    Department of Gastroenterology, Juntendo University, School of Medicine, Tokyo, Japan
    Helicobacter 7:219-24. 2002
    ..Therefore, this issue has remained controversial. Accordingly, we investigated the long-term adverse effects of H. pylori eradication treatment in special reference to development of reflux symptoms...
  39. ncbi request reprint High prevalence of Helicobacter pylori detected by PCR in the oral cavities of periodontitis patients
    Makoto Umeda
    Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    J Periodontol 74:129-34. 2003
    ..The purpose of this study was to use nested polymerase chain reaction (PCR) to clarify whether the oral cavity acts as a reservoir for H. pylori...
  40. pmc Four-week pegylated interferon alpha-2a monotherapy for chronic hepatitis C with genotype 2 and low viral load: a pilot, randomized study
    Akihito Tsubota
    Institute of Clinical Medicine and Research, Jikei University School of Medicine, 163 1 Kashiwa shita, Kashiwa, Chiba 277 8567, Japan
    World J Gastroenterol 14:7220-4. 2008
    ..To assess the efficacy and advantages of 4-wk pegylated interferon alpha-2a (peg-IFN-alpha 2a) monotherapy for chronic hepatitis C patients with strong predictors of sustained virologic response (SVR)...
  41. ncbi request reprint Antibacterial and antimycobacterial treatment for inflammatory bowel disease
    Toshifumi Ohkusa
    Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    J Gastroenterol Hepatol 20:340-51. 2005
    ..Recently, treatments have focused on Gram-negative anaerobes and mycobacteria. The present paper briefly reviews antimicrobial and antimycobacterial treatments in inflammatory bowel disease...
  42. ncbi request reprint Cytotoxicity of organic acids produced by anaerobic intestinal bacteria on cultured epithelial cells
    Toshiaki Sakurazawa
    First Department of Internal Medicine, Tokyo Medical and Dental University School of Medicine, Tokyo, Japan
    J Gastroenterol 40:600-9. 2005
    ..Anaerobic intestinal bacteria produce various organic acids. We identified the concentrations of various organic acids produced by intestinal bacteria needed to kill cultured cells...
  43. doi request reprint Trends of second-line eradication therapy for Helicobacter pylori in Japan: a multicenter study in the Tokyo metropolitan area
    Daisuke Asaoka
    Tokyo Hp Study Group, Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
    Helicobacter 18:468-72. 2013
    ..A possible decrease of the eradication rate for second-line therapy with a PPI, AMPC and metronidazole (MNZ) is of concern. The aim of this study is to assess the trends in second-line eradication therapy for H. pylori in Japan...
  44. doi request reprint Hepatic stellate cells that coexpress LRAT and CRBP-1 partially contribute to portal fibrogenesis in patients with human viral hepatitis
    Keisuke Nagatsuma
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan Department of Pathology, The Jikei University School of Medicine, Tokyo, Japan
    Liver Int 34:243-52. 2014
    ..This study was undertaken to examine whether LRAT+/CRBP-1+ HSCs contribute to portal fibrosis on viral hepatitis...
  45. pmc In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells
    Shigeo Koido
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan
    J Transl Med 6:51. 2008
    ..In this study, generation of cytotoxic T lymphocytes (CTL) and regulatory T cells (Treg) by fusions of dendritic cells (DCs) and HCC cells was examined...