Affiliation: Jikei University School of Medicine
- Development of a real-time PCR assay for the detection and identification of Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus warneriTadayuki Iwase
Department of Microbiology II, Jikei University School of Medicine, 3 25 8 Nishi shinbashi, Minato ku, Tokyo 105 8461, Japan
J Med Microbiol 56:1346-9. 2007..These results indicate that this method is useful for the identification and quantitative analysis of S. capitis, S. haemolyticus and S. warneri...
- Rapid identification and specific quantification of Staphylococcus epidermidis by 5' nuclease real-time polymerase chain reaction with a minor groove binder probeTadayuki Iwase
Department of Bacteriology, The Jikei University School of Medicine, Tokyo 105 8461, Japan
Diagn Microbiol Infect Dis 60:217-9. 2008..This method distinguished S. epidermidis from other staphylococci and specifically quantified the bacterium. This study shows that the method is useful for the identification and quantitative detection of S. epidermidis...
- Isolation and identification of ATP-secreting bacteria from mice and humansTadayuki Iwase
Department of Bacteriology, The Jikei University School of Medicine, and Central Clinical Laboratory, Jikei University Hospital, 3 25 8, Nishi Shimbashi, Minato ku, Tokyo, 105 8461 Japan
J Clin Microbiol 48:1949-51. 2010..However, the ATP-secreting bacteria have not been isolated and identified. In the present study, we report that Enterococcus gallinarum, which is a vancomycin-resistant Gram-positive coccus isolated from mice and humans, secretes ATP...
- Staphylococcus epidermidis Esp inhibits Staphylococcus aureus biofilm formation and nasal colonizationTadayuki Iwase
Department of Bacteriology, The Jikei University School of Medicine, Tokyo, 105 8461 Japan
Nature 465:346-9. 2010..These findings indicate that Esp hinders S. aureus colonization in vivo through a novel mechanism of bacterial interference, which could lead to the development of novel therapeutics to prevent S. aureus colonization and infection...
- Staphylococcus epidermidis Esp degrades specific proteins associated with Staphylococcus aureus biofilm formation and host-pathogen interactionShinya Sugimoto
Department of Bacteriology, The Jikei University School of Medicine, Tokyo, Japan
J Bacteriol 195:1645-55. 2013..These results suggest that Esp inhibits S. aureus colonization and biofilm formation by degrading specific proteins that are crucial for biofilm construction and host-pathogen interaction...
- Role of fibronectin-binding proteins A and B in in vitro cellular infections and in vivo septic infections by Staphylococcus aureusHitomi Shinji
Department of Bacteriology, The Jikei University School of Medicine, 3 25 8 Nishi shinbashi Minato ku, Tokyo 105 8461, Japan
Infect Immun 79:2215-23. 2011..The results showed that FnBPA is more important for in vitro and in vivo infections; however, cooperation between FnBPA and FnBPB is indispensable for the induction of severe infection resulting in septic death...
- Glucose triggers ATP secretion from bacteria in a growth-phase-dependent mannerIppei Hironaka
Department of Bacteriology, The Jikei University School of Medicine, Minato ku, Japan
Appl Environ Microbiol 79:2328-35. 2013..These results suggest that various gut bacteria, including commensals and pathogens, might secrete ATP at any growth phase and modulate immune cell function...
- Inhibition of endothelial interleukin-8 production and neutrophil transmigration by Staphylococcus aureus beta-hemolysinAkiko Tajima
Department of Bacteriology, Jikei University School of Medicine, 3 25 8 Nishi shinbashi, Minato ku, Tokyo 105 8461, Japan
Infect Immun 77:327-34. 2009..These results showed that beta-hemolysin produced by S. aureus interferes with inflammatory signaling in endothelial cells and may help S. aureus evade the host immune response...
- Expression and distribution of very late antigen-5 in mouse peritoneal macrophages upon ingestion of fibronectin-bound Staphylococcus aureusHitomi Shinji
Department of Microbiology II, The Jikei University School of Medicine, Tokyo, Japan
Microbiol Immunol 51:63-71. 2007..Together with our previous work, these results indicate that, upon ingestion of fibronectin-bound S. aureus, VLA-5 accumulates in the area of phagocytosis in inflammatory macrophages, where it forms adhesion complexes...