Takashi Okada

Summary

Affiliation: Jichi Medical School
Country: Japan

Publications

  1. ncbi request reprint Large-scale production of recombinant viruses by use of a large culture vessel with active gassing
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minami Kawachi, Tochigi 329 0498, Japan
    Hum Gene Ther 16:1212-8. 2005
  2. ncbi request reprint In situ generation of pseudotyped retroviral progeny by adenovirus-mediated transduction of tumor cells enhances the killing effect of HSV-tk suicide gene therapy in vitro and in vivo
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    J Gene Med 6:288-99. 2004
  3. ncbi request reprint A histone deacetylase inhibitor enhances recombinant adeno-associated virus-mediated gene expression in tumor cells
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minami Kawachi, Tochigi 329 0498, Japan
    Mol Ther 13:738-46. 2006
  4. ncbi request reprint Intramuscular injection of AAV-GDNF results in sustained expression of transgenic GDNF, and its delivery to spinal motoneurons by retrograde transport
    Yan Yan Lu
    Division of Neurology, Department of Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Tochigi 329 0498, Japan
    Neurosci Res 45:33-40. 2003
  5. ncbi request reprint Utility of intraperitoneal administration as a route of AAV serotype 5 vector-mediated neonatal gene transfer
    Tsuyoshi Ogura
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    J Gene Med 8:990-7. 2006
  6. doi request reprint Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy
    Ryosuke Uchibori
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan
    J Gene Med 11:373-81. 2009
  7. ncbi request reprint Suppression of ovarian cancer by muscle-mediated expression of soluble VEGFR-1/Flt-1 using adeno-associated virus serotype 1-derived vector
    Yuji Takei
    Division of Genetics Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    Int J Cancer 120:278-84. 2007
  8. ncbi request reprint Specific and efficient transduction of Cochlear inner hair cells with recombinant adeno-associated virus type 3 vector
    Yuhe Liu
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minami Kawachi, Kawachi, Tochigi, Japan
    Mol Ther 12:725-33. 2005
  9. ncbi request reprint Interleukin-10 expression mediated by an adeno-associated virus vector prevents monocrotaline-induced pulmonary arterial hypertension in rats
    Takayuki Ito
    Division of Genetic Therapeutics, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke, Tochigi 329 0498, Japan
    Circ Res 101:734-41. 2007
  10. doi request reprint Protection against aminoglycoside-induced ototoxicity by regulated AAV vector-mediated GDNF gene transfer into the cochlea
    Yuhe Liu
    Division of Genetic Therapeutics, Jichi Medical University, Tochigi, Japan
    Mol Ther 16:474-80. 2008

Collaborators

Detail Information

Publications55

  1. ncbi request reprint Large-scale production of recombinant viruses by use of a large culture vessel with active gassing
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minami Kawachi, Tochigi 329 0498, Japan
    Hum Gene Ther 16:1212-8. 2005
    ..Viral production with active gassing is particularly promising, as it can be used with existing incubators and the large culture vessel can readily be converted for use with the active gassing system...
  2. ncbi request reprint In situ generation of pseudotyped retroviral progeny by adenovirus-mediated transduction of tumor cells enhances the killing effect of HSV-tk suicide gene therapy in vitro and in vivo
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    J Gene Med 6:288-99. 2004
    ....
  3. ncbi request reprint A histone deacetylase inhibitor enhances recombinant adeno-associated virus-mediated gene expression in tumor cells
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minami Kawachi, Tochigi 329 0498, Japan
    Mol Ther 13:738-46. 2006
    ..The use of this HDAC inhibitor may enhance the utility of rAAV-mediated transduction strategies for cancer gene therapy...
  4. ncbi request reprint Intramuscular injection of AAV-GDNF results in sustained expression of transgenic GDNF, and its delivery to spinal motoneurons by retrograde transport
    Yan Yan Lu
    Division of Neurology, Department of Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Tochigi 329 0498, Japan
    Neurosci Res 45:33-40. 2003
    ..This study provides a basis for a strategy of intramuscular AAV-GDNF delivery to protect motoneurons as a possible means of ALS treatment...
  5. ncbi request reprint Utility of intraperitoneal administration as a route of AAV serotype 5 vector-mediated neonatal gene transfer
    Tsuyoshi Ogura
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    J Gene Med 8:990-7. 2006
    ..Here, we tested the usefulness of neonatal gene transfer using adeno-associated virus (AAV) vectors by comparing the administration routes and vector doses...
  6. doi request reprint Retroviral vector-producing mesenchymal stem cells for targeted suicide cancer gene therapy
    Ryosuke Uchibori
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan
    J Gene Med 11:373-81. 2009
    ..For efficient therapeutic application, we developed retroviral vector-producing MSCs that enhance tumor transduction via progeny vector production...
  7. ncbi request reprint Suppression of ovarian cancer by muscle-mediated expression of soluble VEGFR-1/Flt-1 using adeno-associated virus serotype 1-derived vector
    Yuji Takei
    Division of Genetics Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan
    Int J Cancer 120:278-84. 2007
    ..These results indicate that sFlt-1 suppresses growth and peritoneal dissemination of ovarian cancer by the inhibition of angiogenesis, and thus suggest the usefulness of gene therapy for ovarian cancer...
  8. ncbi request reprint Specific and efficient transduction of Cochlear inner hair cells with recombinant adeno-associated virus type 3 vector
    Yuhe Liu
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minami Kawachi, Kawachi, Tochigi, Japan
    Mol Ther 12:725-33. 2005
    ..These findings are of value for further molecular studies of cochlear inner hair cells and for gene replacement strategies to correct recessive genetic hearing loss due to monogenic mutations in these cells...
  9. ncbi request reprint Interleukin-10 expression mediated by an adeno-associated virus vector prevents monocrotaline-induced pulmonary arterial hypertension in rats
    Takayuki Ito
    Division of Genetic Therapeutics, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke, Tochigi 329 0498, Japan
    Circ Res 101:734-41. 2007
    ..Thus, IL-10 prevented the development of monocrotaline-induced PAH, and these results provide new insights into the molecular mechanisms of human PAH...
  10. doi request reprint Protection against aminoglycoside-induced ototoxicity by regulated AAV vector-mediated GDNF gene transfer into the cochlea
    Yuhe Liu
    Division of Genetic Therapeutics, Jichi Medical University, Tochigi, Japan
    Mol Ther 16:474-80. 2008
    ..Our results suggest that AAV1-mediated expression of GDNF using a regulated expression system in the cochlea is a promising strategy to protect the cochlea from aminoglycoside-induced damage...
  11. ncbi request reprint Tissue-targeted in vivo gene transfer coupled with histone deacetylase inhibitor depsipeptide (FK228) enhances adenoviral infection in rat renal cancer allograft model systems
    Minoru Kobayashi
    Department of Urology, Jichi Medical University, Tochigi, Japan
    Urology 70:1230-6. 2007
    ..To overcome this disadvantage, we performed in vivo local gene transfer, in combination with the histone deacetylase inhibitor, depsipeptide (FK228), in a rat renal cancer model...
  12. ncbi request reprint Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells
    Takeharu Kanazawa
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji Minamikawachi, Tochigi 329 0498, Japan
    Int J Oncol 25:729-35. 2004
    ..These findings suggest that the combination of AAV-mediated suicide gene therapy and treatment with topoisomerase inhibitors may have synergistic therapeutic effects in the treatment of cancers...
  13. ncbi request reprint Distinct patterns of gene transfer to gerbil hippocampus with recombinant adeno-associated virus type 2 and 5
    Tatsuya Nomoto
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School 3311 1 Yakushiji, Minami Kawachi, Kawachi, Tochigi 329 0498, Japan
    Neurosci Lett 340:153-7. 2003
    ..These findings would be valuable in optimizing rAAV-mediated gene transfer to the gerbil hippocampus...
  14. ncbi request reprint Adenoassociated virus-mediated prostacyclin synthase expression prevents pulmonary arterial hypertension in rats
    Takayuki Ito
    Division of Genetic Therapeutics, Jichi Medical University, Tochigi, Japan
    Hypertension 50:531-6. 2007
    ..An intramuscular injection of AAV-PGIS prevents monocrotaline-pulmonary arterial hypertension in rats and provides a new therapeutic alternative for preventing pulmonary arterial hypertension in humans...
  15. ncbi request reprint Gene transfer to the rat kidney in vivo and ex vivo using an adenovirus vector: factors influencing transgene expression
    Jun Fujishiro
    Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minamikawachi Machi, Tochigi 329 0498, Japan
    Nephrol Dial Transplant 20:1385-91. 2005
    ..We studied the effects of contact time and temperature on adenovirus-mediated transgene expression in rat kidneys, using catheter-based in vivo gene transfer and a rat renal transplant model ex vivo...
  16. doi request reprint Adeno-associated virus vector-mediated systemic interleukin-10 expression ameliorates hypertensive organ damage in Dahl salt-sensitive rats
    Mutsuko Nonaka-Sarukawa
    Division of Genetic Therapeutics, Jichi Medical University, Tochigi, Japan
    J Gene Med 10:368-74. 2008
    ..In the present study, we examined the preventive effects of adeno-associated virus (AAV) vector-mediated sustained IL-10 expression against hypertensive heart disease and renal dysfunction in Dahl salt-sensitive rats...
  17. ncbi request reprint Viral-mediated temporally controlled dopamine production in a rat model of Parkinson disease
    Xiao gang Li
    Division of Neurology, Department of Medicine, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Mol Ther 13:160-6. 2006
    ..Our data demonstrate that viral vector-mediated inducible Cre recombinase can serve as an in vivo molecular switch, allowing spatial and temporal control of transgene expression, thereby potentially increasing the safety of gene therapy...
  18. ncbi request reprint Adipose tissue as a novel target for in vivo gene transfer by adeno-associated viral vectors
    Hiroaki Mizukami
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi 329 0498, Japan
    Hum Gene Ther 17:921-8. 2006
    ..No damage was observed in the transduced tissue. Our results indicate that the proposed method is safe and efficient for gene transfer into adipose tissues, thus providing an alternative for supplemental gene therapy...
  19. ncbi request reprint A soluble CAR-SCF fusion protein improves adenoviral vector-mediated gene transfer to c-Kit-positive hematopoietic cells
    Akira Itoh
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Japan
    J Gene Med 5:929-40. 2003
    ....
  20. ncbi request reprint Development of new inbred transgenic strains of rats with LacZ or GFP
    Hirokazu Inoue
    Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Biochem Biophys Res Commun 329:288-95. 2005
    ..Our Tg-rat system may provide great benefits for the elucidation of the cellular process of regenerative medicine, including cell and tissue transplantation...
  21. ncbi request reprint Adeno-associated virus vectors for gene transfer to the brain
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minami Kawachi, Kawachi, Tochigi 329 0498, Japan
    Methods 28:237-47. 2002
    ....
  22. ncbi request reprint Separate control of Rep and Cap expression using mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production
    Hiroaki Mizukami
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1, Yakushiji, Minamikawachi Machi, Kawachi gun, Tochigi, 329 0498, Japan
    Mol Biotechnol 27:7-14. 2004
    ..1 x 1010 genome copies per 10-cm dish was obtained. These results indicate the importance of controlling Cap expression, in addition to Rep, to achieve an optimum production rate for AAV vectors...
  23. ncbi request reprint Establishment of Cre/LoxP recombination system in transgenic rats
    Yuki Sato
    Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Biochem Biophys Res Commun 319:1197-202. 2004
    ..Taking advantage of the larger body size of the rat than the mouse, this rat system provides a potential value to evaluate biomedical and therapeutic significance for gene therapy and regenerative medicine...
  24. ncbi request reprint Adeno-associated viral vectors for Parkinson's disease
    Shin Ichi Muramatsu
    Division of Neurology, Jichi Medical School, Tochigi 329 0498, Japan
    Int Rev Neurobiol 55:205-22. 2003
  25. ncbi request reprint Prevention of diabetic retinopathy by intraocular soluble flt-1 gene transfer in a spontaneously diabetic rat model
    Junichi Ideno
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi 329 0498, Japan
    Int J Mol Med 19:75-9. 2007
    ..No adverse effects were observed throughout the study. These results indicate that local sflt-1 gene transfer inhibits DR progression in SDT rats and offers powerful therapeutic potential for the management of human DR...
  26. ncbi request reprint Neuroprotective effects of glial cell line-derived neurotrophic factor mediated by an adeno-associated virus vector in a transgenic animal model of amyotrophic lateral sclerosis
    Li Jun Wang
    Division of Neurology, Department of Medicine, Center for Molecular Medicine, Jichi Medical School, Minamikawachi Machi, Tochigi 329 0498, Japan
    J Neurosci 22:6920-8. 2002
    ..Our finding thus indicates that AAV-mediated GDNF delivery to the muscle is a promising means of gene therapy for ALS...
  27. ncbi request reprint Persistent phenotypic correction of central diabetes insipidus using adeno-associated virus vector expressing arginine-vasopressin in Brattleboro rats
    Junichi Ideno
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Mol Ther 8:895-902. 2003
    ....
  28. ncbi request reprint In vivo expansion of transduced murine hematopoietic cells with a selective amplifier gene
    Akihiro Kume
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    J Gene Med 5:175-81. 2003
    ..Prototype SAGs conferred estrogen-responsive growth on murine hematopoietic progenitors...
  29. ncbi request reprint Selective expansion of transduced cells for hematopoietic stem cell gene therapy
    Akihiro Kume
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Minamikawachi, Tochigi, Japan
    Int J Hematol 76:299-304. 2002
    ..This review aims at an overview of recent advances involving these strategies, with some details of "selective amplifier genes," a novel system that we have developed for specific expansion of genetically modified hematopoietic cells...
  30. ncbi request reprint Reduction of CTLL-2 cytotoxicity by induction of apoptosis with a Fas-estrogen receptor chimera
    Minako Kametaka
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi 329 0498, Japan
    Cancer Sci 94:639-43. 2003
    ..The feasibility of using the MfasER-estrogen system to control graft-versus-host disease was demonstrated...
  31. ncbi request reprint Resistin induces insulin resistance in pancreatic islets to impair glucose-induced insulin release
    Masanori Nakata
    Division of Integrative Physiology, Department of Physiology, Jichi Medical University, School of Medicine, Shimotsuke, Tochigi 329 0498, Japan
    Biochem Biophys Res Commun 353:1046-51. 2007
    ..In conclusion, resistin induces insulin resistance in islet beta-cells at least partly via induction of SOCS-3 expression and reduction of Akt phosphorylation and impairs glucose-induced insulin secretion...
  32. doi request reprint Cell and gene therapy using mesenchymal stem cells (MSCs)
    Keiya Ozawa
    Division of Hematology, Department of Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke shi, Tochigi 329 0498, Japan
    J Autoimmun 30:121-7. 2008
    ..4) by using the integration machinery of adeno-associated virus (AAV) would be particularly valuable. There will be wide-ranging applications of MSCs to frontier medical treatments in the near future...
  33. ncbi request reprint Interleukin-10-mediated inhibition of angiogenesis and tumor growth in mice bearing VEGF-producing ovarian cancer
    Takahiro Kohno
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi, 329 0498 Japan
    Cancer Res 63:5091-4. 2003
    ..Although the mechanisms of the antiangiogenic effect of IL-10 are still unclear, the potential usefulness of IL-10-mediated gene therapy of ovarian cancer was suggested...
  34. ncbi request reprint Phenotype correction of hemophilia A mice with adeno-associated virus vectors carrying the B domain-deleted canine factor VIII gene
    Akira Ishiwata
    Division of Cell and Molecular Medicine, The Center for Molecular Medicine, Jichi Medical School, Tochigi ken, Japan
    Thromb Res 118:627-35. 2006
    ..These data suggested that both AAV1 and AAV8 vectors carrying the FVIII gene utilizing a minimum promoter have a potential for hemophilia A gene therapy...
  35. ncbi request reprint Adeno-associated viral vector-mediated gene therapy of ischemia-induced neuronal death
    Takashi Okada
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Kawachi, Tochigi 329 0498, Japan
    Methods Enzymol 346:378-93. 2002
  36. ncbi request reprint The adenovirus E1A and E1B19K genes provide a helper function for transfection-based adeno-associated virus vector production
    Takashi Matsushita
    Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, 3311 1 Yakushiji, Minami Kawachi, Kawachi, Tochigi 329 0489, Japan
    J Gen Virol 85:2209-14. 2004
    ..These findings may be valuable for the future development of packaging cell lines for AAV vector production...
  37. ncbi request reprint Antiapoptotic effect of endothelin-1 in rat cardiomyocytes in vitro
    Yukiyo Ogata
    Division of Cardiovascular Medicine, Department of Medicine, Jichi Medical School, Tochigi, Japan
    Hypertension 41:1156-63. 2003
    ..These findings suggest that ET-1 prevents apoptosis in cardiac myocytes through the ETA receptor and the subsequent c-Src/Bcl-xL-dependent pathway...
  38. ncbi request reprint Vector-producing tumor-tracking multipotent mesenchymal stromal cells for suicide cancer gene therapy
    Takashi Okada
    Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan
    Front Biosci 13:1887-91. 2008
    ..While this strategy remains to be tested in various orthotopic or metastatic tumor models, it has the potential to greatly improve the outcome of suicide gene therapy...
  39. doi request reprint Recombinant adeno-associated virus type 8-mediated extensive therapeutic gene delivery into skeletal muscle of alpha-sarcoglycan-deficient mice
    Akiyo Nishiyama
    Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187 8502, Japan
    Hum Gene Ther 19:719-30. 2008
    ..This extensive rAAV8-mediated alpha-SG transduction in LGMD 2D model animals paves the way for future clinical application...
  40. ncbi request reprint Long-term potentiation in the hippocampal CA1 area and dentate gyrus plays different roles in spatial learning
    Takashi Okada
    Department of Molecular Neuroscience, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Eur J Neurosci 17:341-9. 2003
    ..Thus, it was demonstrated that CA1 and dentate gyrus synapses play different functional roles in spatial learning despite their similar mechanism for LTP induction...
  41. ncbi request reprint Promoter effects of adeno-associated viral vector for transgene expression in the cochlea in vivo
    Yuhe Liu
    Department of Otolaryngology, Head and Neck Surgery, Peking University First Hospital, Beijing 100034, China
    Exp Mol Med 39:170-5. 2007
    ..Our results might provide important information with regard to the role of promoters in regulating transgene expression and for the proper design of vectors for gene expression and gene therapy...
  42. ncbi request reprint Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells
    Aki Mizutani
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Cancer Res 64:3144-7. 2004
    ..These data suggest a contribution of TLS to the prevention of chromosomal breaks by TAM and estrogen, and they therefore indicate that such error-prone DNA synthesis underlies mutagenesis induced by these agents...
  43. pmc Multiple roles of vertebrate REV genes in DNA repair and recombination
    Takashi Okada
    Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Kyoto 606 8501, Japan
    Mol Cell Biol 25:6103-11. 2005
    ..The present study gives us a new insight into cooperative function of three Rev molecules and the Polzeta (Rev3-Rev7)-independent role of Rev1 in vertebrate cells...
  44. ncbi request reprint Involvement of vertebrate polkappa in Rad18-independent postreplication repair of UV damage
    Takashi Okada
    Department of Radiation Genetics, Kyoto University Graduate School of Medicine, Japan
    J Biol Chem 277:48690-5. 2002
    ..This implies functional redundancy between HR and Polkappa in maintaining chromosomal DNA. In conclusion, vertebrate Polkappa is involved in Rad18-independent TLS of UV damage and plays a role in maintaining genomic stability...
  45. pmc RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells
    Yukiko M Yamashita
    Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    EMBO J 21:5558-66. 2002
    ..These data suggest that RAD18 plays an essential role in maintaining chromosomal DNA in cooperation with the RAD54-dependent DNA repair pathway...
  46. ncbi request reprint A new noninvasive measurement system for wave intensity: evaluation of carotid arterial wave intensity and reproducibility
    Kiyomi Niki
    Department of Cardiovascular Sciences, The Heart Institute of Japan, Tokyo Women s Medical University School of Medicine, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Heart Vessels 17:12-21. 2002
    ..However, two sessions by the same observer and two sessions by different observers were not biased. Wave intensity measurements with this system are clinically acceptable...
  47. ncbi request reprint On-line noninvasive one-point measurements of pulse wave velocity
    Akimitsu Harada
    Research Laboratory, Aloka Co, Ltd, 3 7 19 Imai, Ome, Tokyo 198 8577, Japan
    Heart Vessels 17:61-8. 2002
    ..899 (R(2) = 0.83). Although regional PWV in the human carotid artery has not been reported so far, the correlation between the PWVs obtained by the present two methods was so high that we are convinced of the validity of these methods...
  48. ncbi request reprint [Development of automatic verification system for portal image in radiotherapy]
    Hiroyuki Akazawa
    Clinical Radiology Service, Kyoto University Hospital
    Nihon Hoshasen Gijutsu Gakkai Zasshi 60:101-10. 2004
    ..Using this software, the direction of shift was detected correctly, and the distance of shift was detected correctly to within less than 3 mm. We consider that this software could be a useful method of verification...
  49. ncbi request reprint rAAV-mediated shRNA ameliorated neuropathology in Huntington disease model mouse
    Yoko Machida
    Laboratory for Structural Neuropathology, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 343:190-7. 2006
    ..These results suggest that the direct inhibition of mutant gene expression by rAVV would be promising for post-symptomatic HD therapy...
  50. ncbi request reprint Regional differences in GABAergic modulation for TEA-induced synaptic plasticity in rat hippocampal CA1, CA3 and dentate gyrus
    Etsuko Suzuki
    Department of Psychology, Graduate School of the Humanities, Senshu University, 2 1 1 Higashimita, Tama, Kawasaki, Kanagawa 214 8580, Japan
    Neurosci Res 59:183-90. 2007
    ..This series of results demonstrates that TEA-induced DG and CA3 plasticity displays afferent specificity and is exposed to GABAergic modulation in an opposite manner...
  51. ncbi request reprint Histone H3 variants in male gametic cells of lily and H3 methylation in mature pollen
    Takashi Okada
    Plant Molecular Biology and Biotechnology Laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, The University of Melbourne, Parkville, Victoria 3010, Australia
    Plant Mol Biol 62:503-12. 2006
    ..Taken together, these results suggest that male gametic cells of Lilium have unique chromatin state and histone H3 variants and their methylation might be involved in gene regulation of male gametic cells...
  52. pmc Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage
    Michio Yoshimura
    Department of Radiation Genetics, CREST, Japan Science and Technology Laboratory, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Mol Cell 24:115-25. 2006
    ..Accordingly, POLQ and POLbeta share an overlapping function in the repair of oxidative base damage. Taken together, these results suggest a role for vertebrate POLQ in BER...
  53. pmc Multiple roles of Rev3, the catalytic subunit of polzeta in maintaining genome stability in vertebrates
    Eiichiro Sonoda
    CREST Research Project, Japan Science and Technology, Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    EMBO J 22:3188-97. 2003
    ..In conclusion, Rev3 plays critical roles in PRR, which accounts for survival on naturally occurring endogenous as well as induced damages during replication...
  54. ncbi request reprint Clinical usefulness of carotid arterial wave intensity in assessing left ventricular systolic and early diastolic performance
    Nobuyuki Ohte
    Department of Internal Medicine and Pathophysiology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho cho, Mizuho ku, Nagoya 467 8601, Japan
    Heart Vessels 18:107-11. 2003
    ..WI is a noninvasively obtained, clinically useful parameter for the evaluation of LV systolic and early diastolic performance at the same time...
  55. ncbi request reprint Involvement of vertebrate Polkappa in translesion DNA synthesis across DNA monoalkylation damage
    Katsuya Takenaka
    Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Biol Chem 281:2000-4. 2006
    ..These data imply that Polkappa has a function in TLS past alkylated base adducts as well as UV radiation DNA damage in vertebrates...