Research Topics
Genomes and GenesSpecies
| JCR Pharmaceuticals CoSummaryOrganization: JCR Pharmaceuticals Co Country: Japan Top Publications
| Scientific Experts
|
Detail Information
Publications
The cleavage site specificity of human prostate specific antigen for insulin-like growth factor binding protein-3E Okabe
Development and Research Laboratories, JCR Pharmaceuticals, Kobe, Japan
FEBS Lett 447:87-90. 1999..In contrast, our purified urinary prostate specific antigen had only a chymotrypsin-like activity, demonstrating that prostate specific antigen has the high substrate specificity for human insulin-like growth factor binding protein-3...- Efficient production of active Vibrio proteolyticus aminopeptidase in Escherichia coli by co-expression with engineered vibriolysinHiroyuki Sonoda
Research Center, Research Division, JCR Pharmaceuticals Co, Ltd, Kobe 651 2241, Japan
Appl Microbiol Biotechnol 84:191-8. 2009..This purified aminopeptidase effectively converted methionyl-human growth hormone (Met-hGH) to hGH. Thus, this co-expression system provides an efficient method for producing active recombinant V. proteolyticus aminopeptidase... - Improved solubilization of recombinant human growth hormone inclusion body produced in Escherichia coliHiroyuki Sonoda
Medical Technology Research Center, Research Division, JCR Pharmaceuticals Co, Ltd, Kobe, Japan
Biosci Biotechnol Biochem 72:2675-80. 2008..The biological activity of the resulting r-hGH was comparable with commercially available r-hGH in in vitro cell proliferation assay using the hGH-dependent cell line... - Extracellular production of active vibriolysin engineered by random mutagenesis in Escherichia coliHiroyuki Sonoda
Research Division, Medical Technology Research Center, JCR Pharmaceuticals Co, Ltd, Kobe, Japan
Protein Expr Purif 62:153-9. 2008..2mg/L of purified protein. This suggests that the novel engineered vibriolysin is useful for overproduction in an E. coli expression system... - Characterization of group a streptococcal M23 protein and comparison of the M3 and M23 protein's ligand-binding domainsKyongsu Hong
Laboratories for Bioengineering and Research, JCR Pharmaceuticals Co, Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
Curr Microbiol 55:427-34. 2007.... - Characterization of the arginine deiminase of Streptococcus equi subsp. zooepidemicusKyongsu Hong
Laboratories for Bioengineering and Research, JCR Pharmaceuticals Co, Ltd, Kobe, Japan
Can J Microbiol 52:868-76. 2006..The recombinant protein also possessed AD activity. By immunoblot analysis using both anti-SzSAGP-SfbI(H8) and anti-SfbI(H8) sera, I was able to demonstrate that the SzSAGP protein is expressed on the streptococcal surface... - Identification and characterization of a novel fibronectin-binding protein gene from Streptococcus equi subspecies zooepidemicus strain VTU211Kyongsu Hong
Laboratories for Bioengineering and Research, JCR Pharmaceuticals Co, Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
FEMS Immunol Med Microbiol 45:231-7. 2005..Purified FNZ2(2-4) protein was also shown to display collagen-binding activity to collagen-coated microtiter wells. These results show that recombinant FNZ2 has fibronectin- and collagen-binding activities... - Protein C inhibitor as an anti-disseminated intravascular coagulation agent--mechanism and modificationM Fujita
Pharmacological Science, Research Center, Research and Development Division, JCR Pharmaceuticals Co Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
Curr Med Chem Cardiovasc Hematol Agents 2:21-7. 2004..This review introduces the anti-DIC action of PCI and about the modification of N-glycosylation site(s) of PCI to heighten the value of PCI as an anti-DIC agent... - Development of a high-throughput bioassay to screen melatonin receptor agonists using human melatonin receptor expressing CHO cellsTetsuo Yokoyama
JCR Pharmaceuticals Co, Ltd, Pharmaceutical Science, Research Center, Research and Development Division, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
Neurosci Lett 344:45-8. 2003..Therefore, this FLIPR assay, using melatonin receptor and Gqi5 expressing CHO cells, is available for clinical bioassay of melatonin and for the screening of specific ligands of melatonin... - Role of each Asn-linked glycan in the anticoagulant activity of human protein C inhibitorMitsugu Fujita
Development and Research Laboratories, JCR Pharmaceuticals Co, Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
Thromb Res 105:95-102. 2002..These results suggest that the Asn243-linked glycan in PCI molecule possesses critical roles for its anticoagulant activity in the circulation, and the Asn230-linked glycan down-regulates the activity of PCI... - The trimannosyl cores of N-glycans are important for the procoagulant protease-inhibitory activity of urinary protein C inhibitorW Izutani
Development and Research Laboratories, JCR Pharmaceuticals Co, Ltd, 2 2 10 Murotani, Nishi ku, Kobe 651 2241, Japan
Thromb Res 104:65-74. 2001..These results suggest that the trimannosyl cores of N-glycans are important for uPCI to inhibit the procoagulant protease... - Amino-terminal fragment of urokinase-type plasminogen activator inhibits HIV-1 replicationM Wada
Laboratories for Bioengineering and Research, JCR Pharmaceuticals Company, Ltd, 2 2 10 Murotani, Nishi ku, Kobe, 651 2241, Japan
Biochem Biophys Res Commun 284:346-51. 2001..These findings suggest that ATF may inhibit the assembly and budding of HIV-1, which provides a novel therapeutic strategy for AIDS... - Production of a recombinant hybrid molecule of cholera toxin-B-subunit and proteolipid-protein-peptide for the treatment of experimental encephalomyelitisY Yuki
JCR Pharmaceuticals Co, 2 2 10 Murotani, Nishi ku, Kobe 651 2241 Japan
Biotechnol Bioeng 74:62-9. 2001..These results suggest that nasal treatment with the recombinant B. brevis-derived hybrid protein of CTB and autoantigen peptide could prove useful in the control of multiple sclerosis... - Effect of urinary protein C inhibitor on lipopolysaccharide-induced disseminated intravascular coagulation in ratsM Fujita
Development and Research Laboratories, JCR Pharmaceuticals Co, Ltd, Kobe, Japan
Thromb Haemost 84:54-8. 2000..These results show that uPCI may prevent hypercoagulation, the induction of secondary fibrinolysis and organ failure in the DIC model. Therefore, uPCI may be a useful agent for the clinical treatment of DIC... - Characterization of group A streptococcal strains Sv and Su: determination of emm gene typing and presence of small vir regulonK Hong
Laboratories for Bioengineering and Research, ICR Pharmaceuticals Co, Ltd, Kobe, Japan
Res Microbiol 151:29-36. 2000..Taken together, the data suggest that M protein could be expressed on the surface of the Sv bacteria, but not on the Su bacteria... - Isolation of a fission yeast mutant cell affected in MAP kinase signaling and sterol biosynthesisKiwamu Imagawa
Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan
Kobe J Med Sci 55:E30-5. 2009....