S Aono

Summary

Affiliation: Japan Advanced Institute of Science and Technology
Country: Japan

Publications

  1. ncbi A novel heme protein that acts as a carbon monoxide-dependent transcriptional activator in Rhodospirillum rubrum
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan
    Biochem Biophys Res Commun 228:752-6. 1996
  2. ncbi Resonance Raman and ligand binding studies of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis
    Shigetoshi Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1 1 Asahidai, Tatsunokuchi, Ishikawa, Japan
    J Biol Chem 277:13528-38. 2002
  3. ncbi CO sensing and regulation of gene expression by the transcriptional activator CooA
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi gun, Ishikawa
    J Inorg Biochem 82:51-6. 2000
  4. ncbi Recognition of target DNA and transcription activation by the CO-sensing transcriptional activator CooA
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1 1 Asahidai, Tatsunokuchi, Ishikawa, Nomi gun, 923 1292, Japan
    Biochem Biophys Res Commun 261:270-5. 1999
  5. ncbi Single transduction in the transcriptional activator CooA containing a heme-based CO sensor: isolation of a dominant positive mutant which is active as the transcriptional activator even in the absence of CO
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan
    Biochem Biophys Res Commun 240:783-6. 1997
  6. ncbi Dissociation and recombination between ligands and heme in a CO-sensing transcriptional activator CooA. A flash photolysis study
    S Kumazaki
    School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa 923 1292, Japan
    J Biol Chem 275:38378-83. 2000
  7. ncbi Redox-controlled ligand exchange of the heme in the CO-sensing transcriptional activator CooA
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1 1 Asahidai, Tatsunokuchi, Nomi gun, Ishikawa 923 1292, Japan
    J Biol Chem 273:25757-64. 1998
  8. ncbi Redox properties and coordination structure of the heme in the co-sensing transcriptional activator CooA
    H Nakajima
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatsunokuchi, Nomi-gun, Ishikawa 923-1292, Japan
    J Biol Chem 276:7055-61. 2001
  9. ncbi Conformational dynamics of the transcriptional regulator CooA protein studied by subpicosecond mid-infrared vibrational spectroscopy
    I V Rubtsov
    School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa 923 1292, Japan
    J Am Chem Soc 123:10056-62. 2001
  10. ncbi Ligand-switching intermediates for the CO-sensing transcriptional activator CooA measured by pulse radiolysis
    H Nakajima
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatsunokuchi, Nomi-gun, Ishikawa 923-1292, Japan
    J Biol Chem 276:37895-9. 2001

Collaborators

Detail Information

Publications29

  1. ncbi A novel heme protein that acts as a carbon monoxide-dependent transcriptional activator in Rhodospirillum rubrum
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan
    Biochem Biophys Res Commun 228:752-6. 1996
    ..CO-bound CooA was formed when reduced CooA was reacted with CO, but not in the case of oxidized CooA. CooA is the first example of the heme protein acting as a DNA-binding transcriptional activator...
  2. ncbi Resonance Raman and ligand binding studies of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis
    Shigetoshi Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1 1 Asahidai, Tatsunokuchi, Ishikawa, Japan
    J Biol Chem 277:13528-38. 2002
    ..Based on these results, the structural and functional properties of HemAT-Bs are discussed...
  3. ncbi CO sensing and regulation of gene expression by the transcriptional activator CooA
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi gun, Ishikawa
    J Inorg Biochem 82:51-6. 2000
    ....
  4. ncbi Recognition of target DNA and transcription activation by the CO-sensing transcriptional activator CooA
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1 1 Asahidai, Tatsunokuchi, Ishikawa, Nomi gun, 923 1292, Japan
    Biochem Biophys Res Commun 261:270-5. 1999
    ..The properties of the CRP/CooA chimeric proteins constructed in this work suggest that CooA activates transcription by a similar mechanism to that of CRP at Class II CRP-dependent promoters...
  5. ncbi Single transduction in the transcriptional activator CooA containing a heme-based CO sensor: isolation of a dominant positive mutant which is active as the transcriptional activator even in the absence of CO
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan
    Biochem Biophys Res Commun 240:783-6. 1997
    ..Methionine at position 131 was the carboxyl-terminal end of the heme-binding domain of CooA, which would be adjacent to the hinge region connecting the heme-binding domain and the DNA-binding domain...
  6. ncbi Dissociation and recombination between ligands and heme in a CO-sensing transcriptional activator CooA. A flash photolysis study
    S Kumazaki
    School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa 923 1292, Japan
    J Biol Chem 275:38378-83. 2000
    ..9 ns. The dynamics of rebinding of CO thus will help us to understand how the physiologically relevant diatomic molecule approaches the heme binding site in CooA with picosecond resolution...
  7. ncbi Redox-controlled ligand exchange of the heme in the CO-sensing transcriptional activator CooA
    S Aono
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1 1 Asahidai, Tatsunokuchi, Nomi gun, Ishikawa 923 1292, Japan
    J Biol Chem 273:25757-64. 1998
    ..NMR spectra reveal that the conformational change around the heme, which will trigger the activation of CooA by CO, takes place upon the binding of CO to the heme...
  8. ncbi Redox properties and coordination structure of the heme in the co-sensing transcriptional activator CooA
    H Nakajima
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatsunokuchi, Nomi-gun, Ishikawa 923-1292, Japan
    J Biol Chem 276:7055-61. 2001
    ..The redox-controlled ligand exchange of the heme between Cys(75) and His(77) is thought to cause the difference between the reduction and oxidation midpoint potentials...
  9. ncbi Conformational dynamics of the transcriptional regulator CooA protein studied by subpicosecond mid-infrared vibrational spectroscopy
    I V Rubtsov
    School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa 923 1292, Japan
    J Am Chem Soc 123:10056-62. 2001
    ..Thus, the biologically relevant conformation change in CooA was traced. Possible assignment of the observed conformation change is discussed...
  10. ncbi Ligand-switching intermediates for the CO-sensing transcriptional activator CooA measured by pulse radiolysis
    H Nakajima
    School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatsunokuchi, Nomi-gun, Ishikawa 923-1292, Japan
    J Biol Chem 276:37895-9. 2001
    ..9 ms (k = 3.5 x 10(2) x s(-1)) for wild-type CooA. The ligand exchange between Cys75 and His77 took place during the conversion of the second intermediate into the final reduced form...
  11. ncbi Recognition and discrimination of gases by the oxygen-sensing signal transducer protein HemAT as revealed by FTIR spectroscopy
    Eftychia Pinakoulaki
    Department of Chemistry, University of Crete, 71409 Heraklion, Crete, Greece
    Biochemistry 45:7763-6. 2006
    ....
  12. ncbi Specific hydrogen-bonding networks responsible for selective O2 sensing of the oxygen sensor protein HemAT from Bacillus subtilis
    Hideaki Yoshimura
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji, Okazaki, Aichi 444 8787, Japan
    Biochemistry 45:8301-7. 2006
    ..This stepwise conformational change would be essential to selective O2 sensing and signal transduction by HemAT-Bs...
  13. ncbi Effect of mutation on the dissociation and recombination dynamics of CO in transcriptional regulator CooA: a picosecond infrared transient absorption study
    Tieqiao Zhang
    School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa 923 1292, Japan
    Biochemistry 45:9246-53. 2006
    ....
  14. pmc Two ligand-binding sites in the O2-sensing signal transducer HemAT: implications for ligand recognition/discrimination and signaling
    Eftychia Pinakoulaki
    Department of Chemistry, University of Crete, Heraklion, 710 03 Voutes, Crete, Greece
    Proc Natl Acad Sci U S A 103:14796-801. 2006
    ....
  15. ncbi Systematic regulation of the enzymatic activity of phenylacetaldoxime dehydratase by exogenous ligands
    Katsuaki Kobayashi
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji, Okazaki 444 8787, Japan
    Chembiochem 7:2004-9. 2006
    ..Kinetic analyses revealed that the proximal His regulated not only the affinity of substrate binding to the heme but also the elimination of the OH group from the substrate...
  16. ncbi Crystal structure of CO-sensing transcription activator CooA bound to exogenous ligand imidazole
    Hirofumi Komori
    Department of Life Science, Graduate School of Life Science, University of Hyogo and Himeji Institute of Technology, 3 2 1 Koto, Kamigori cho, Ako gun, Hyogo 678 1297, Japan
    J Mol Biol 367:864-71. 2007
    ..These results indicate that the release of the N terminus resulting from imidazole binding is not sufficient to activate CooA. The structure provides new insights into the structural changes required to achieve activation...
  17. ncbi The formation of hydrogen bond in the proximal heme pocket of HemAT-Bs upon ligand binding
    Hideaki Yoshimura
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji, Okazaki, Aichi 444 8787, Japan
    Biochem Biophys Res Commun 357:1053-7. 2007
    ..We discuss a functional role of this hydrogen bond formation for the signal transduction in HemAT-Bs...
  18. ncbi Evidence for displacements of the C-helix by CO ligation and DNA binding to CooA revealed by UV resonance Raman spectroscopy
    Minoru Kubo
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444 8787, Japan
    J Biol Chem 281:11271-8. 2006
    ..Moreover, CO-geminate recombination was more efficient in the DNA-bound state. These results suggest that the C-helix displacement in the DNA-bound form causes the CO binding pocket to narrow and become more negative...
  19. ncbi Protein conformation changes of HemAT-Bs upon ligand binding probed by ultraviolet resonance Raman spectroscopy
    Samir F El-Mashtoly
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444 8787, Japan
    J Biol Chem 283:6942-9. 2008
    ..Accordingly, the present UVRR results suggest that O2 binding to heme causes displacement of the G-helix, which would be important for transduction of the conformational changes from the sensor domain to the signaling domain...
  20. ncbi Biochemical and biophysical properties of the CO-sensing transcriptional activator CooA
    Shigetoshi Aono
    Center for Integrative Bioscience, Okazaki National Research Institutes, 38 Nishigo naka, Myodaiji, Okazaki 444 8585, Japan
    Acc Chem Res 36:825-31. 2003
    ..The mechanisms of CO sensing by CooA and CO-dependent activation of CooA have now been analyzed by both molecular biological and spectroscopic studies and are discussed in this Account...
  21. ncbi Resonance Raman and ligand-binding analysis of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis
    Shigetoshi Aono
    Center for Integrative Bioscience, Okazaki National Research Institutes, Japan
    Methods Enzymol 381:618-28. 2004
  22. ncbi Activation mechanisms of transcriptional regulator CooA revealed by small-angle X-ray scattering
    Shuji Akiyama
    RIKEN Harima Institute SPring 8, Structural Biochemistry Laboratory, 1 1 1 Kouto, Mikazuki, Sayo, Hyogo 679 5148, Japan
    J Mol Biol 341:651-68. 2004
    ..The CO-dependent structural change observed by SAXS corresponds to a slight swing of the DNA-binding domains away from the heme domains coupled with their rotation by about 8 degrees around the axis of 2-fold symmetry...
  23. ncbi Spectroscopic and redox properties of a CooA homologue from Carboxydothermus hydrogenoformans
    Sayaka Inagaki
    Department of Structural Molecule Science, The Graduate University for Advanced Studies, 38 Nishigo naka, Myodaiji, Okazaki 444 8585, Japan
    J Biol Chem 280:3269-74. 2005
    ....
  24. ncbi Oxygen-sensing mechanism of HemAT from Bacillus subtilis: a resonance Raman spectroscopic study
    Takehiro Ohta
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444 8787, Japan
    J Am Chem Soc 126:15000-1. 2004
    ..A scheme for specific O2 sensing and signaling mechanism is discussed...
  25. ncbi Biophysical properties of a c-type heme in chemotaxis signal transducer protein DcrA
    Shiro Yoshioka
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5 1, Higashiyama, Myodaiji, Okazaki 444 8787, Japan
    Biochemistry 44:15406-13. 2005
    ..While the affinity of CO to DcrA-N (Kd = 138 microM) is significantly weak compared to those of other heme proteins, we suggest that CO might be another physiological effector molecule...
  26. ncbi Regulation of aldoxime dehydratase activity by redox-dependent change in the coordination structure of the aldoxime-heme complex
    Katsuaki Kobayashi
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji, Okazaki 444 8787, Japan
    J Biol Chem 280:5486-90. 2005
    ..The pH dependence of OxdB activity suggested that another amino acid residue that assists the elimination of the OH group of PAOx would work as a catalytic residue along with His-306...
  27. pmc Crystallization and preliminary X-ray analysis of CooA from Carboxydothermus hydrogenoformans
    Hirofumi Komori
    Department of Life Science, Graduate School of Life Science, University of Hyogo, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:471-3. 2006
    ..7, c = 92.8 angstroms, beta = 104.8 degrees. The native and anomalous difference Patterson maps indicated that two CooA dimers are contained in the asymmetric unit and are related by a translational symmetry almost parallel to the c axis...
  28. ncbi Characterization of the heme environmental structure of cytoglobin, a fourth globin in humans
    Hitomi Sawai
    Department of Life Science, Graduate School of Science, Himeji Institute of Technology, 3 2 1 Kouto, Kamigori cho, Ako, Hyogo 678 1297, Japan
    Biochemistry 42:5133-42. 2003
    ..These structural properties of the heme pocket of Cgb are discussed with respect to its proposed in vivo oxygen storage function...
  29. ncbi Spectroscopic and substrate binding properties of heme-containing aldoxime dehydratases, OxdB and OxdRE
    Katsuaki Kobayashi
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5 1 Higashiyama, Myodaiji, Okazaki 444 8787, Japan
    J Inorg Biochem 100:1069-74. 2006
    ..Electronic paramagnetic resonance (EPR) and rapid scanning spectroscopy revealed that the flexibility of the heme pocket was different between OxdB and OxdRE, which might affect their substrate specificity...