Research Topics
Genomes and Genes
| M FujitaSummaryAffiliation: Institute for Developmental Research Country: Japan Publications
| Collaborators
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Detail Information
Publications
Cell cycle regulation of DNA replication initiation proteins in mammalian cellsM Fujita
Masatoshi Fujita, Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Kanokoden 1 1, Chikusa ku, Nagoya 464 8681, Japan
Front Biosci 4:D816-23. 1999..A hypothetical model for the state of prereplication chromatin in mammalian cell nuclei and regulation during the cell cycle is also proposed...- Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinaseM Fujita
Laboratories of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya, 464, Japan
J Biol Chem 273:17095-101. 1998.... - Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylationM Fujita
Laboratories of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464 8681, Japan
J Biol Chem 274:25927-32. 1999..However, co-immunoprecipitation assays with chemical cross-linking indicated that a small part of the chromatin-bound MCM is present close to the bound CDC6... - In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATPM Fujita
Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
J Biol Chem 272:10928-35. 1997..Nuclease digestion-immunoprecipitation techniques of the type described here should facilitate further elucidation of the mode of interaction between hMCMs and chromatin... - hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phaseM Fujita
Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
J Biol Chem 271:4349-54. 1996..Together, these findings indicate that hCDC47 protein together with other MCM proteins participates in the regulation of mammalian DNA replication... - cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cellsY Hayashi
Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
J Biol Chem 272:18082-6. 1997..The results allow us to conclude that cca1 is required but not sufficient for formation of growth-arrested confluent monolayer of 3Y1 cells... - Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM familyT Kiyono
Laboratory of Viral Oncology, Research Institute, Aichi Cancer Centre, Nagoya, Japan
Biochim Biophys Acta 1307:31-4. 1996..These results indicate that the cDNA covers the entire coding region of a human homologue of CDC47... 
Binding of high-risk human papillomavirus E6 oncoproteins to the human homologue of the Drosophila discs large tumor suppressor proteinT Kiyono
Laboratory of Viral Oncology, Aichi Cancer Center, Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464, Japan
Proc Natl Acad Sci U S A 94:11612-6. 1997..These results suggest an intriguing possibility that interaction between the E6 protein and hDLG or other PDZ domain-containing proteins could be an underlying mechanism in the development of HPV-associated cancers...- Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cellsY Hayashi
Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya, Japan
Biochim Biophys Acta 1352:145-50. 1997..The CCA2 protein, with a molecular weight of 38 kDa, was accumulated in 3Y1 cells under growth-arrest conditions... - Overexpression, purification and helix-destabilizing properties of Epstein-Barr virus ssDNA-binding proteinT Tsurumi
Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Nagoya, Japan
J Gen Virol 79:1257-64. 1998..The helix-destabilizing property of the EBV SSB may function to melt out secondary structures on the ssDNA template, thereby facilitating the movement of the EBV DNA polymerase... - Increased frequency of antigen-specific CD8(+) cytotoxic T lymphocytes infiltrating an Epstein-Barr virus-associated gastric carcinomaK Kuzushima
Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Nagoya 464 0021, Japan
J Clin Invest 104:163-71. 1999..In addition, our data suggest that class I-restricted, antigen-specific CD8(+) CTLs are specifically expanded within EBV(+) gastric carcinoma tissue... - Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensationH Goto
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
J Biol Chem 274:25543-9. 1999..These observations suggest that H3 phosphorylation at Ser(28), together with Ser(10), is a conserved event and is likely to be involved in mitotic chromosome condensation... - Acceleration of wound contraction and healing with a photocrosslinkable chitosan hydrogelM Ishihara
Research Institute, and Departments of Surgery II, Orthopedic Surgery, and Medical Engineering, National Defense Medical College, and NeTech Inc, Tokorozawa, Saitama, Japan
Wound Repair Regen 9:513-21. 2001..Due to its ability to accelerate wound contraction and healing, chitosan hydrogel may become accepted as an occlusive dressing for wound management... - Cell cycle dependent topological changes of chromosomal replication origins in Saccharomyces cerevisiaeM Fujita
Nara Institute of Science and Technology, Japan
Genes Cells 3:737-49. 1998..Further studies on topological changes of replication origin in vivo caused by ORC association are crucial for an understanding of chromosomal DNA replication in S. cerevisiae... - Immunolocalization of hCDC47 protein in normal and neoplastic human tissues and its relation to growthA Hiraiwa
Department of Dermatology, Nagoya University School of Medicine, Japan
Int J Cancer 74:180-4. 1997..Our findings indicate that hCDC47 plays a role in normal and neoplastic cell growth in vivo and that hCDC47 immunolocalization could be used as an index of cell proliferation in tissue sections... - Interactions and nuclear import of the N and P proteins of sonchus yellow net virus, a plant nucleorhabdovirusM M Goodin
Department of Plant and Microbial Biology, University of California, Berkeley, 94720, USA
J Virol 75:9393-406. 2001..Taken together, our results support a model for independent entry of N and P into the nucleus followed by associations that mediate subnuclear localization... - Up-regulation of the ectodermal-neural cortex 1 (ENC1) gene, a downstream target of the beta-catenin/T-cell factor complex, in colorectal carcinomasM Fujita
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, Japan
Cancer Res 61:7722-6. 2001..These data suggest that ENC1 is regulated by the beta-catenin/Tcf pathway and that its altered expression may contribute to colorectal carcinogenesis by suppressing differentiation of colonic cells... - Cloning and overproduction of biodegradative threonine deaminase from Escherichia coli W strainK Hirose
Department of Chemistry, Faculty of Science, Kyoto University, Japan
Biotechnol Appl Biochem 15:134-41. 1992..Amino acid sequence analysis of this recombinant enzyme showed that the amino acid sequence is identical to the nucleotide-deduced sequence of biodegradative threonine deaminase from E. coli K-12... - Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acidH Okamura
Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 3-18-15, Kuramoto, Tokushima 770-8504, Japan
Oral Oncol 38:779-84. 2002..The present results indicate that the alteration of phosphorylation states in SCCKN cells regulates Egr-1 binding to its consensus sequence and its expression at the transcriptional level... - Effect of active immunization against inhibin on hormonal concentrations and semen characteristics in Shiba bucksM S Medan
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan
Theriogenology 65:691-702. 2006..Active immunization against inhibin could be used to improve fertility in Shiba bucks... - HPV16 E6-mediated stabilization of ErbB2 in neoplastic transformation of human cervical keratinocytesM Narisawa-Saito
Virology Division, National Cancer Center Research Institute, Tokyo, Japan
Oncogene 26:2988-96. 2007..Taken together, these data indicate an important role of ErbB2 regulation by HPV16 E6 in oncogenic transformation of human cervical keratinocytes... - Identification of AF17 as a downstream gene of the beta-catenin/T-cell factor pathway and its involvement in colorectal carcinogenesisY M Lin
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
Cancer Res 61:6345-9. 2001..These findings should aid development of new strategies for diagnosis, treatment, and prevention of colon cancers and acute leukemias by clarifying the pathogenesis of these conditions... - Isolation and characterization of human NBL4, a gene involved in the beta-catenin/tcf signaling pathwayH Ishiguro
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
Jpn J Cancer Res 91:597-603. 2000..The results support the view that NBL4 is an important component of the beta-catenin / Tcf pathway and is probably related to determination of cell polarity or proliferation... - AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1S Satoh
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
Nat Genet 24:245-50. 2000.... - Cloning and sequencing of rpoH and identification of ftsE-ftsX in Pseudomonas putida PpG1H Aramaki
Department of Molecular Biology, Daiichi College of Pharmaceutical Sciences, Fukuoka, Fukuoka, Japan
DNA Res 6:241-5. 1999..aeruginosa and E. coli. Moreover, homologs to the cell division genes ftsX and ftsE were found immediately upstream of the rpoH gene... - Isolation and characterization of a human cDNA homologous to the Xenopus laevis XCAP-C gene belonging to the structural maintenance of chromosomes (SMC) familyT Nishiwaki
Laboratory of Molecular Medicine, Institute of Medical Science, University of Tokyo, Japan
J Hum Genet 44:197-202. 1999..We also determined its chromosomal location at 3q26.1 by fluorescence in situ hybridization... - Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in JapanM Fujita
Biotechnology Research Laboratories, JCR Pharmaceuticals Co, Ltd, Kobe, Japan
Biochem Biophys Res Commun 197:1340-7. 1993..PMSF inhibited both the fibrinolytic activity and the amidolytic activity. The results indicate that nattokinase is a subtilisin-like serine protease... - Gene structure of CYP3A4, an adult-specific form of cytochrome P450 in human livers, and its transcriptional controlH Hashimoto
Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
Eur J Biochem 218:585-95. 1993.... - Novel zinc chelators with dual activity in the inhibition of the kappa B site-binding proteins HIV-EP1 and NF-kappa BM Otsuka
Institute for Chemical Research, Kyoto University, Japan
J Med Chem 38:3264-70. 1995..Thus, it became possible to inhibit either one of the two kappa B site-binding proteins without inhibiting the other.(ABSTRACT TRUNCATED AT 250 WORDS).. - Phosphorylation of beta-glucuronidases from human normal liver and hepatoma by cAMP-dependent protein kinaseM Ono
Biochemistry Laboratory, Hokkaido University School of Medicine, Sapporo, Japan
J Biol Chem 263:5884-9. 1988..32P-labeled beta-glucuronidase indicated that the 64 kDa subunit was phosphorylated both in hepatoma and normal liver beta-glucuronidases... 
Mitochondrial association of alpha-synuclein causes oxidative stressM S Parihar
Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University, 460 West 12th Avenue, Columbus, 43210 OH, USA
Cell Mol Life Sci 65:1272-84. 2008..These findings suggest a pivotal role for mitochondria in oxidative stress and apoptosis induced by alpha-synuclein...