M Fujita

Summary

Affiliation: Institute for Developmental Research
Country: Japan

Publications

  1. ncbi Cell cycle regulation of DNA replication initiation proteins in mammalian cells
    M Fujita
    Masatoshi Fujita, Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Kanokoden 1 1, Chikusa ku, Nagoya 464 8681, Japan
    Front Biosci 4:D816-23. 1999
  2. ncbi Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinase
    M Fujita
    Laboratories of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya, 464, Japan
    J Biol Chem 273:17095-101. 1998
  3. ncbi Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation
    M Fujita
    Laboratories of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464 8681, Japan
    J Biol Chem 274:25927-32. 1999
  4. ncbi In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP
    M Fujita
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
    J Biol Chem 272:10928-35. 1997
  5. ncbi hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase
    M Fujita
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
    J Biol Chem 271:4349-54. 1996
  6. ncbi cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cells
    Y Hayashi
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
    J Biol Chem 272:18082-6. 1997
  7. ncbi Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family
    T Kiyono
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Centre, Nagoya, Japan
    Biochim Biophys Acta 1307:31-4. 1996
  8. pmc Binding of high-risk human papillomavirus E6 oncoproteins to the human homologue of the Drosophila discs large tumor suppressor protein
    T Kiyono
    Laboratory of Viral Oncology, Aichi Cancer Center, Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464, Japan
    Proc Natl Acad Sci U S A 94:11612-6. 1997
  9. ncbi Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells
    Y Hayashi
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya, Japan
    Biochim Biophys Acta 1352:145-50. 1997
  10. ncbi Overexpression, purification and helix-destabilizing properties of Epstein-Barr virus ssDNA-binding protein
    T Tsurumi
    Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Nagoya, Japan
    J Gen Virol 79:1257-64. 1998

Collaborators

Detail Information

Publications31

  1. ncbi Cell cycle regulation of DNA replication initiation proteins in mammalian cells
    M Fujita
    Masatoshi Fujita, Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Kanokoden 1 1, Chikusa ku, Nagoya 464 8681, Japan
    Front Biosci 4:D816-23. 1999
    ..A hypothetical model for the state of prereplication chromatin in mammalian cell nuclei and regulation during the cell cycle is also proposed...
  2. ncbi Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinase
    M Fujita
    Laboratories of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya, 464, Japan
    J Biol Chem 273:17095-101. 1998
    ....
  3. ncbi Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation
    M Fujita
    Laboratories of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464 8681, Japan
    J Biol Chem 274:25927-32. 1999
    ..However, co-immunoprecipitation assays with chemical cross-linking indicated that a small part of the chromatin-bound MCM is present close to the bound CDC6...
  4. ncbi In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP
    M Fujita
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
    J Biol Chem 272:10928-35. 1997
    ..Nuclease digestion-immunoprecipitation techniques of the type described here should facilitate further elucidation of the mode of interaction between hMCMs and chromatin...
  5. ncbi hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase
    M Fujita
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
    J Biol Chem 271:4349-54. 1996
    ..Together, these findings indicate that hCDC47 protein together with other MCM proteins participates in the regulation of mammalian DNA replication...
  6. ncbi cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cells
    Y Hayashi
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya 464, Japan
    J Biol Chem 272:18082-6. 1997
    ..The results allow us to conclude that cca1 is required but not sufficient for formation of growth-arrested confluent monolayer of 3Y1 cells...
  7. ncbi Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family
    T Kiyono
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Centre, Nagoya, Japan
    Biochim Biophys Acta 1307:31-4. 1996
    ..These results indicate that the cDNA covers the entire coding region of a human homologue of CDC47...
  8. pmc Binding of high-risk human papillomavirus E6 oncoproteins to the human homologue of the Drosophila discs large tumor suppressor protein
    T Kiyono
    Laboratory of Viral Oncology, Aichi Cancer Center, Research Institute, 1 1 Kanokoden, Chikusa ku, Nagoya 464, Japan
    Proc Natl Acad Sci U S A 94:11612-6. 1997
    ..These results suggest an intriguing possibility that interaction between the E6 protein and hDLG or other PDZ domain-containing proteins could be an underlying mechanism in the development of HPV-associated cancers...
  9. ncbi Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells
    Y Hayashi
    Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa ku, Nagoya, Japan
    Biochim Biophys Acta 1352:145-50. 1997
    ..The CCA2 protein, with a molecular weight of 38 kDa, was accumulated in 3Y1 cells under growth-arrest conditions...
  10. ncbi Overexpression, purification and helix-destabilizing properties of Epstein-Barr virus ssDNA-binding protein
    T Tsurumi
    Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Nagoya, Japan
    J Gen Virol 79:1257-64. 1998
    ..The helix-destabilizing property of the EBV SSB may function to melt out secondary structures on the ssDNA template, thereby facilitating the movement of the EBV DNA polymerase...
  11. pmc Increased frequency of antigen-specific CD8(+) cytotoxic T lymphocytes infiltrating an Epstein-Barr virus-associated gastric carcinoma
    K Kuzushima
    Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Nagoya 464 0021, Japan
    J Clin Invest 104:163-71. 1999
    ..In addition, our data suggest that class I-restricted, antigen-specific CD8(+) CTLs are specifically expanded within EBV(+) gastric carcinoma tissue...
  12. ncbi Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation
    H Goto
    Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
    J Biol Chem 274:25543-9. 1999
    ..These observations suggest that H3 phosphorylation at Ser(28), together with Ser(10), is a conserved event and is likely to be involved in mitotic chromosome condensation...
  13. ncbi Acceleration of wound contraction and healing with a photocrosslinkable chitosan hydrogel
    M Ishihara
    Research Institute, and Departments of Surgery II, Orthopedic Surgery, and Medical Engineering, National Defense Medical College, and NeTech Inc, Tokorozawa, Saitama, Japan
    Wound Repair Regen 9:513-21. 2001
    ..Due to its ability to accelerate wound contraction and healing, chitosan hydrogel may become accepted as an occlusive dressing for wound management...
  14. ncbi Cell cycle dependent topological changes of chromosomal replication origins in Saccharomyces cerevisiae
    M Fujita
    Nara Institute of Science and Technology, Japan
    Genes Cells 3:737-49. 1998
    ..Further studies on topological changes of replication origin in vivo caused by ORC association are crucial for an understanding of chromosomal DNA replication in S. cerevisiae...
  15. ncbi Immunolocalization of hCDC47 protein in normal and neoplastic human tissues and its relation to growth
    A Hiraiwa
    Department of Dermatology, Nagoya University School of Medicine, Japan
    Int J Cancer 74:180-4. 1997
    ..Our findings indicate that hCDC47 plays a role in normal and neoplastic cell growth in vivo and that hCDC47 immunolocalization could be used as an index of cell proliferation in tissue sections...
  16. pmc Interactions and nuclear import of the N and P proteins of sonchus yellow net virus, a plant nucleorhabdovirus
    M M Goodin
    Department of Plant and Microbial Biology, University of California, Berkeley, 94720, USA
    J Virol 75:9393-406. 2001
    ..Taken together, our results support a model for independent entry of N and P into the nucleus followed by associations that mediate subnuclear localization...
  17. ncbi Up-regulation of the ectodermal-neural cortex 1 (ENC1) gene, a downstream target of the beta-catenin/T-cell factor complex, in colorectal carcinomas
    M Fujita
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108 8639, Japan
    Cancer Res 61:7722-6. 2001
    ..These data suggest that ENC1 is regulated by the beta-catenin/Tcf pathway and that its altered expression may contribute to colorectal carcinogenesis by suppressing differentiation of colonic cells...
  18. ncbi Cloning and overproduction of biodegradative threonine deaminase from Escherichia coli W strain
    K Hirose
    Department of Chemistry, Faculty of Science, Kyoto University, Japan
    Biotechnol Appl Biochem 15:134-41. 1992
    ..Amino acid sequence analysis of this recombinant enzyme showed that the amino acid sequence is identical to the nucleotide-deduced sequence of biodegradative threonine deaminase from E. coli K-12...
  19. ncbi Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid
    H Okamura
    Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 3 18 15, Kuramoto, Tokushima 770 8504, Japan
    Oral Oncol 38:779-84. 2002
    ..The present results indicate that the alteration of phosphorylation states in SCCKN cells regulates Egr-1 binding to its consensus sequence and its expression at the transcriptional level...
  20. ncbi Effect of active immunization against inhibin on hormonal concentrations and semen characteristics in Shiba bucks
    M S Medan
    Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo 183 8509, Japan
    Theriogenology 65:691-702. 2006
    ..Active immunization against inhibin could be used to improve fertility in Shiba bucks...
  21. ncbi HPV16 E6-mediated stabilization of ErbB2 in neoplastic transformation of human cervical keratinocytes
    M Narisawa-Saito
    Virology Division, National Cancer Center Research Institute, Tokyo, Japan
    Oncogene 26:2988-96. 2007
    ..Taken together, these data indicate an important role of ErbB2 regulation by HPV16 E6 in oncogenic transformation of human cervical keratinocytes...
  22. ncbi Identification of AF17 as a downstream gene of the beta-catenin/T-cell factor pathway and its involvement in colorectal carcinogenesis
    Y M Lin
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan
    Cancer Res 61:6345-9. 2001
    ..These findings should aid development of new strategies for diagnosis, treatment, and prevention of colon cancers and acute leukemias by clarifying the pathogenesis of these conditions...
  23. ncbi Isolation and characterization of human NBL4, a gene involved in the beta-catenin/tcf signaling pathway
    H Ishiguro
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Jpn J Cancer Res 91:597-603. 2000
    ..The results support the view that NBL4 is an important component of the beta-catenin / Tcf pathway and is probably related to determination of cell polarity or proliferation...
  24. ncbi AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1
    S Satoh
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Nat Genet 24:245-50. 2000
    ....
  25. ncbi Cloning and sequencing of rpoH and identification of ftsE-ftsX in Pseudomonas putida PpG1
    H Aramaki
    Department of Molecular Biology, Daiichi College of Pharmaceutical Sciences, Fukuoka, Fukuoka, Japan
    DNA Res 6:241-5. 1999
    ..aeruginosa and E. coli. Moreover, homologs to the cell division genes ftsX and ftsE were found immediately upstream of the rpoH gene...
  26. ncbi Isolation and characterization of a human cDNA homologous to the Xenopus laevis XCAP-C gene belonging to the structural maintenance of chromosomes (SMC) family
    T Nishiwaki
    Laboratory of Molecular Medicine, Institute of Medical Science, University of Tokyo, Japan
    J Hum Genet 44:197-202. 1999
    ..We also determined its chromosomal location at 3q26.1 by fluorescence in situ hybridization...
  27. ncbi Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan
    M Fujita
    Biotechnology Research Laboratories, JCR Pharmaceuticals Co, Ltd, Kobe, Japan
    Biochem Biophys Res Commun 197:1340-7. 1993
    ..PMSF inhibited both the fibrinolytic activity and the amidolytic activity. The results indicate that nattokinase is a subtilisin-like serine protease...
  28. ncbi Gene structure of CYP3A4, an adult-specific form of cytochrome P450 in human livers, and its transcriptional control
    H Hashimoto
    Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Eur J Biochem 218:585-95. 1993
    ....
  29. ncbi Novel zinc chelators with dual activity in the inhibition of the kappa B site-binding proteins HIV-EP1 and NF-kappa B
    M Otsuka
    Institute for Chemical Research, Kyoto University, Japan
    J Med Chem 38:3264-70. 1995
    ..Thus, it became possible to inhibit either one of the two kappa B site-binding proteins without inhibiting the other.(ABSTRACT TRUNCATED AT 250 WORDS)..
  30. ncbi Phosphorylation of beta-glucuronidases from human normal liver and hepatoma by cAMP-dependent protein kinase
    M Ono
    Biochemistry Laboratory, Hokkaido University School of Medicine, Sapporo, Japan
    J Biol Chem 263:5884-9. 1988
    ..32P-labeled beta-glucuronidase indicated that the 64 kDa subunit was phosphorylated both in hepatoma and normal liver beta-glucuronidases...
  31. doi Mitochondrial association of alpha-synuclein causes oxidative stress
    M S Parihar
    Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University, 460 West 12th Avenue, Columbus, 43210 OH, USA
    Cell Mol Life Sci 65:1272-84. 2008
    ..These findings suggest a pivotal role for mitochondria in oxidative stress and apoptosis induced by alpha-synuclein...