S Nakajin

Summary

Affiliation: Hoshi University
Country: Japan

Publications

  1. ncbi request reprint Deletion of 12 carboxyl-terminal residues from pig 3alpha/beta,20beta-hydroxysteroid dehydrogenase affects steroid metabolism
    S Nakajin
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2 4 41, Ebara, Shinagawa ku, Tokyo 142, Japan
    Biochim Biophys Acta 1550:175-82. 2001
  2. ncbi request reprint 20 beta-hydroxysteroid dehydrogenase of neonatal pig testis: 3 alpha/beta-hydroxysteroid dehydrogenase activities catalyzed by highly purified enzyme
    S Ohno
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    J Steroid Biochem Mol Biol 38:787-94. 1991
  3. ncbi request reprint [Purification and some properties of 3 alpha-hydroxysteroid dehydrogenase from pig adrenal cytosol]
    S Nakajin
    Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Yakugaku Zasshi 111:775-82. 1991
  4. ncbi request reprint Ontogeny of testicular steroid dehydrogenase enzymes in pig (3 alpha/beta-, 20 alpha- and 20 beta-): evidence for two forms of 3 alpha/beta-hydroxysteroid dehydrogenase
    S Ohno
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    J Steroid Biochem Mol Biol 42:17-21. 1992
  5. ncbi request reprint 20 beta-hydroxysteroid dehydrogenase of neonatal pig testis: reverse catalytic (oxidation) reaction
    S Ohno
    Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Chem Pharm Bull (Tokyo) 39:972-5. 1991
  6. ncbi request reprint Purification and characterization of 3 alpha/beta-hydroxysteroid dehydrogenase from mature porcine testicular cytosol
    S Nakajin
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    J Steroid Biochem Mol Biol 48:249-56. 1994
  7. ncbi request reprint Immunochemical distribution and immunohistochemical localization of 20beta-hydroxysteroid dehydrogenase in neonatal pig tissues
    K Kobayashi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Ebara, Shinagawa ku, Tokyo, Japan
    J Steroid Biochem Mol Biol 59:485-93. 1996
  8. ncbi request reprint An NADPH-dependent reductase in neonatal pig testes that metabolizes androgens and xenobiotics
    S Nakajin
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Biol Pharm Bull 21:1356-60. 1998
  9. ncbi request reprint [Direct expression of the pig testicular 3 alpha/beta(20 beta)- hydroxysteroid dehydrogenase cDNA using baculovirus expression system]
    S Nakajin
    Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Yakugaku Zasshi 115:344-50. 1995
  10. ncbi request reprint Leydig cells from neonatal pig testis abundantly express 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 2 and effectively inactivate cortisol to cortisone
    Y Honda
    Department of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa, Tokyo, Japan
    J Steroid Biochem Mol Biol 108:91-101. 2008

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Deletion of 12 carboxyl-terminal residues from pig 3alpha/beta,20beta-hydroxysteroid dehydrogenase affects steroid metabolism
    S Nakajin
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2 4 41, Ebara, Shinagawa ku, Tokyo 142, Japan
    Biochim Biophys Acta 1550:175-82. 2001
    ....
  2. ncbi request reprint 20 beta-hydroxysteroid dehydrogenase of neonatal pig testis: 3 alpha/beta-hydroxysteroid dehydrogenase activities catalyzed by highly purified enzyme
    S Ohno
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    J Steroid Biochem Mol Biol 38:787-94. 1991
    ....
  3. ncbi request reprint [Purification and some properties of 3 alpha-hydroxysteroid dehydrogenase from pig adrenal cytosol]
    S Nakajin
    Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Yakugaku Zasshi 111:775-82. 1991
    ..These results strongly suggest that 3 alpha-HSD purified from pig adrenal cytosol is a bi-functional enzyme catalyzing 3 alpha- and 20 alpha-HSD activities...
  4. ncbi request reprint Ontogeny of testicular steroid dehydrogenase enzymes in pig (3 alpha/beta-, 20 alpha- and 20 beta-): evidence for two forms of 3 alpha/beta-hydroxysteroid dehydrogenase
    S Ohno
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    J Steroid Biochem Mol Biol 42:17-21. 1992
    ..The possible functional significance of these changes is considered...
  5. ncbi request reprint 20 beta-hydroxysteroid dehydrogenase of neonatal pig testis: reverse catalytic (oxidation) reaction
    S Ohno
    Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Chem Pharm Bull (Tokyo) 39:972-5. 1991
    ..8, 27.0 and 20.0 kcal/mol, respectively...
  6. ncbi request reprint Purification and characterization of 3 alpha/beta-hydroxysteroid dehydrogenase from mature porcine testicular cytosol
    S Nakajin
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    J Steroid Biochem Mol Biol 48:249-56. 1994
    ..g. pI, effects of various inhibitors and greater enzyme activity towards steroids (neonatal form) or prostaglandins (mature form). Reasons are give for suggesting that these enzymes are closely related to carbonyl reductase...
  7. ncbi request reprint Immunochemical distribution and immunohistochemical localization of 20beta-hydroxysteroid dehydrogenase in neonatal pig tissues
    K Kobayashi
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Ebara, Shinagawa ku, Tokyo, Japan
    J Steroid Biochem Mol Biol 59:485-93. 1996
    ....
  8. ncbi request reprint An NADPH-dependent reductase in neonatal pig testes that metabolizes androgens and xenobiotics
    S Nakajin
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Biol Pharm Bull 21:1356-60. 1998
    ..Interestingly, we could not find multiple isoforms of 3alpha/beta,20beta-hydroxysteroid dehydrogenase/carbonyl reductase in pig testes unlike human and rat testes and other organs in which multiple isoforms are expressed...
  9. ncbi request reprint [Direct expression of the pig testicular 3 alpha/beta(20 beta)- hydroxysteroid dehydrogenase cDNA using baculovirus expression system]
    S Nakajin
    Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
    Yakugaku Zasshi 115:344-50. 1995
    ..Furthermore, it had 3-keto reductase activity of 3 alpha/beta(20 beta)-HSD for 5 alpha-dihydrotestosterone and 20-keto reductase activity for 17 alpha-hydroxyprogesterone in the presence of NADPH...
  10. ncbi request reprint Leydig cells from neonatal pig testis abundantly express 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 2 and effectively inactivate cortisol to cortisone
    Y Honda
    Department of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa, Tokyo, Japan
    J Steroid Biochem Mol Biol 108:91-101. 2008
    ....
  11. ncbi request reprint Forskolin up-regulates aromatase (CYP19) activity and gene transcripts in the human adrenocortical carcinoma cell line H295R
    M Watanabe
    Department of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa, Tokyo 142 8501, Japan
    J Endocrinol 180:125-33. 2004
    ..The results of this work should provide the basis for the molecular analysis of aromatase expression in adrenocortical cells...
  12. ncbi request reprint Aromatase expression in a human osteoblastic cell line increases in response to prostaglandin E(2) in a dexamethasone-dependent fashion
    M Watanabe
    Department of Biochemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa, Tokyo 142 8501, Japan
    Steroids 72:686-92. 2007
    ..4 to drive aromatase expression. These findings also suggest that endogenous PGE(2) produced in bone mainly may synergistically support local estrogen production in osteoblastic cells in the presence of glucocorticoid...
  13. ncbi request reprint Amino terminal sequence analysis of human placenta aromatase
    S Chen
    Biochem Biophys Res Commun 135:713-9. 1986
    ..The results indicate that aromatase is a cytochrome p-450 protein, probably derived from a new cytochrome p-450 family...
  14. ncbi request reprint Aromatase expression in the human fetal osteoblastic cell line SV-HFO
    M Watanabe
    Prince Henry s Institute of Medical Research, PO Box 5152, Clayton, VIC 3168, Australia
    J Mol Endocrinol 32:533-45. 2004
    ..These data suggest that the SV-HFO cell line is a valuable model with which to elucidate the mechanisms regulating local estrogen synthesis in osteoblasts...