Mutsumi Takagi

Summary

Affiliation: Hokkaido University
Country: Japan

Publications

  1. doi request reprint Correlation between actin content and laser phase shift of adhesive normal and malignant prostate epithelial cells
    Mutsumi Takagi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13 W8 Kita ku, Sapporo, Japan
    J Biosci Bioeng 115:310-3. 2013
  2. doi request reprint Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells
    Mutsumi Takagi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 114:556-9. 2012
  3. pmc In vitro Proliferation of Human Bone Marrow Mesenchymal Stem Cells Employing Donor Serum and Basic Fibroblast Growth Factor
    Mutsumi Takagi
    International Center for Biotechnology, Osaka University, 2 1, Yamada oka, Suita, Osaka, 565 0871, Japan
    Cytotechnology 43:89-96. 2003
  4. doi request reprint Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes
    Mutsumi Takagi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, 060 8628, Japan
    Biotechnol Lett 30:1189-95. 2008
  5. ncbi request reprint Noninvasive measurement of three-dimensional morphology of adhered animal cells employing phase-shifting laser microscope
    Mutsumi Takagi
    Hokkaido University, Graduate School of Engineering, Division of Biotechnology and Macromolecular Chemistry, Sapporo 060 8628, Japan
    J Biomed Opt 12:054010. 2007
  6. ncbi request reprint Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells
    Mutsumi Takagi
    International Center for Biotechnology, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biosci Bioeng 101:223-6. 2006
  7. doi request reprint Shrinkage-free preparation of scaffold-free cartilage-like disk-shaped cell sheet using human bone marrow mesenchymal stem cells
    Satoshi Maeda
    Hokkaido University, Kita ku N13W8, Sapporo 060 8628, Japan
    J Biosci Bioeng 111:489-92. 2011
  8. ncbi request reprint Cell processing engineering for ex-vivo expansion of hematopoietic cells
    Mutsumi Takagi
    Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 99:189-96. 2005
  9. doi request reprint A mass separation of chondrocytes from cartilage tissue utilizing an automatic crushing device
    Mutsumi Takagi
    Hokkaido University, Sapporo, Japan
    J Biosci Bioeng 109:73-4. 2010
  10. ncbi request reprint Xeno-free and shrinkage-free preparation of scaffold-free cartilage-like disc-shaped cell sheet using human bone marrow mesenchymal stem cells
    Yasushi Sato
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita ku, N13W8, Sapporo 060 8628, Japan
    J Biosci Bioeng 116:734-9. 2013

Collaborators

Detail Information

Publications30

  1. doi request reprint Correlation between actin content and laser phase shift of adhesive normal and malignant prostate epithelial cells
    Mutsumi Takagi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13 W8 Kita ku, Sapporo, Japan
    J Biosci Bioeng 115:310-3. 2013
    ..Consequently, the smaller height of PC-3 cells caused by lower actin content than of PRECs might be the reason for the lower phase shift of PC-3 cells...
  2. doi request reprint Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells
    Mutsumi Takagi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 114:556-9. 2012
    ..In conclusion, it was proposed that adherent PC-3 cancer cells could be noninvasively discriminated from normal adherent PRECs by the periodical measurement of phase shift using PLM during culture...
  3. pmc In vitro Proliferation of Human Bone Marrow Mesenchymal Stem Cells Employing Donor Serum and Basic Fibroblast Growth Factor
    Mutsumi Takagi
    International Center for Biotechnology, Osaka University, 2 1, Yamada oka, Suita, Osaka, 565 0871, Japan
    Cytotechnology 43:89-96. 2003
    ..Consequently, this method might be applicable to in vitro proliferation of MSCs for the regeneration of cartilage...
  4. doi request reprint Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes
    Mutsumi Takagi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, 060 8628, Japan
    Biotechnol Lett 30:1189-95. 2008
    ..Approximately 80% of the large polygonal cells were negative for MSC marker (CD90, CD166) expression and the aggrecan mRNA expression level of the large polygonal cells was markedly higher than that of cells with other morphologies...
  5. ncbi request reprint Noninvasive measurement of three-dimensional morphology of adhered animal cells employing phase-shifting laser microscope
    Mutsumi Takagi
    Hokkaido University, Graduate School of Engineering, Division of Biotechnology and Macromolecular Chemistry, Sapporo 060 8628, Japan
    J Biomed Opt 12:054010. 2007
    ..Laser irradiation, which is necessary for the observation under PLM, did not affect 3-D cell morphology. In conclusion, 3-D morphology of adhered animal cells can be noninvasively measured under PLM...
  6. ncbi request reprint Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells
    Mutsumi Takagi
    International Center for Biotechnology, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biosci Bioeng 101:223-6. 2006
    ..5 M. Addition of 0.5 or 1.0 M NaCl at mid-log phase or the end of log phase during cultivation with initial NaCl concentration of 1.0 M further increased the lipid content (70%)...
  7. doi request reprint Shrinkage-free preparation of scaffold-free cartilage-like disk-shaped cell sheet using human bone marrow mesenchymal stem cells
    Satoshi Maeda
    Hokkaido University, Kita ku N13W8, Sapporo 060 8628, Japan
    J Biosci Bioeng 111:489-92. 2011
    ..In conclusion, the usage of CCI having 12% porosity and 18.6 × 10(5)MSCs/well could avoid the shrinkage from the formation of the scaffold-free cartilage-like disk-shaped cell sheet...
  8. ncbi request reprint Cell processing engineering for ex-vivo expansion of hematopoietic cells
    Mutsumi Takagi
    Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 99:189-96. 2005
    ..Heparin addition to the 3-D coculture and coating the nonwoven fabrics with N-(O-beta-(6-O-sulfogalactopyranosyl)-6-oxyhexyl)-3,5-bis(dodecyloxy)-benzamide further increased the number of progenitors...
  9. doi request reprint A mass separation of chondrocytes from cartilage tissue utilizing an automatic crushing device
    Mutsumi Takagi
    Hokkaido University, Sapporo, Japan
    J Biosci Bioeng 109:73-4. 2010
    ..Chondrocyte cell yield was maximized by automatic crushing for 10 s, which was 4 times that by manual dicing with a surgical blade and might be useful for the automation of the cell processing...
  10. ncbi request reprint Xeno-free and shrinkage-free preparation of scaffold-free cartilage-like disc-shaped cell sheet using human bone marrow mesenchymal stem cells
    Yasushi Sato
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita ku, N13W8, Sapporo 060 8628, Japan
    J Biosci Bioeng 116:734-9. 2013
    ..The scaffold-free and xeno-free cartilage-like cell sheet was successfully formed without shrinkage using human bone marrow MSCs and the chondrogenic differentiation medium containing human serum...
  11. ncbi request reprint High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells
    Mutsumi Takagi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 103:98-100. 2007
    ..7-fold that in the case of the culture with an initial MSC concentration of 0.05 x 10(4) cells/cm(2). Thus, a high MSC inoculum density might be appropriate for the rapid differentiation of MSCs to chondrocytes...
  12. doi request reprint Ex vivo cartilage defect model for the evaluation of cartilage regeneration using mesenchymal stem cells
    Ryosuke Iwai
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita ku, N13W8, Sapporo 060 8628, Japan
    J Biosci Bioeng 111:357-64. 2011
    ..In conclusion, a chondral disc with defects is useful for evaluating cartilage regeneration using MSCs...
  13. ncbi request reprint Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes
    Shohei Nishimoto
    International Center for Biotechnology, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Biosci Bioeng 100:123-6. 2005
    ..Hyaluronic acid of high molecular weight markedly decreased the mRNA expression of both aggrecan and type 2 collagen, although hyaluronic acid of low molecular weight showed no apparent effect...
  14. doi request reprint Correlation between cell cycle phase of adherent Chinese hamster ovary cells and laser phase shift determined by phase-shifting laser microscopy
    Syunsuke Ito
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo 060 8628, Japan
    Biotechnol Lett 31:39-42. 2009
    ....
  15. doi request reprint Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum
    Masashi Fujiwara
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 109:307-9. 2010
    ..An increase in FS concentration from 1% to 4% markedly increased cell density. On the other hand, heat treatment of FS showed nearly no effect on cell density...
  16. doi request reprint Influence of fetal calf serum on differentiation of mesenchymal stem cells to chondrocytes during expansion
    Mari Yokoyama
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita ku N13W8, Sapporo 060 8628, Japan
    J Biosci Bioeng 106:46-50. 2008
    ..Consequently, FCS components could stimulate MSC differentiation to chondrocytes and a lower concentration could decrease this differentiation...
  17. doi request reprint Construction of an osteochondral-like tissue graft combining β-tricalcium phosphate block and scaffold-free mesenchymal stem cell sheet
    Shigeharu Miyagi
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita ku, Sapporo, Hokkaido, 060 8628, Japan
    J Orthop Sci 18:471-7. 2013
    ..Aiming to construct an osteochondral-like structure, the combination of a β-tricalcium phosphate (βTCP) block with a scaffold-free sheet formed using mesenchymal stem cells (MSCs) was investigated...
  18. doi request reprint Noninvasive discrimination of human normal cells and malignant tumor cells by phase-shifting laser microscopy
    Ayako Tokumitsu
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 109:499-503. 2010
    ..In conclusion, these adherent cancer cells can be noninvasively discriminated from normal adherent cells by phase shift measurement using PLM, and the sensitivity of the method of detecting cancer cell contamination reached 10%...
  19. doi request reprint Construction of osteochondral-like tissue graft combining β-tricalcium phosphate block and scaffold-free centrifuged chondrocyte cell sheet
    Kouhei Niyama
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita ku N13W8, Sapporo, 060 8628, Japan
    J Orthop Sci 16:613-21. 2011
    ..The combination of a β-tricalcium phosphate (βTCP) block with a scaffold-free chondrocyte sheet formed by the centrifugation of chondrocytes in a well was investigated with the aim of constructing an osteochondral-like structure...
  20. doi request reprint Quantitative correlation between production rate of melanoma inhibitory activity and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes and redifferentiation of chondrocytes
    Kaori Onoue
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita ku, Sapporo, Japan
    J Biosci Bioeng 111:594-6. 2011
    ....
  21. doi request reprint Combination of cytomegalovirus enhancer with human cellular promoters for gene-induced chondrogenesis of human bone marrow mesenchymal stem cells
    Ryosuke Iwai
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 110:593-6. 2010
    ..MSCs transfected with the vector showed higher transgene production of a cytokine, which increased the differentiation level to chondrocytes...
  22. doi request reprint Effect of monosaccharides composing glycosaminoglycans on type 2 collagen accumulation in a three-dimensional culture of chondrocytes
    Erina Kagita
    Hokkaido University, Sapporo, Japan
    J Biosci Bioeng 109:51-4. 2010
    ..In conclusion, the addition of monosaccharides composing GAGs might be valuable for increasing COL(II) accumulation in the 3D culture of chondrocytes...
  23. ncbi request reprint Effect of heparin addition on expansion of cord blood hematopoietic progenitor cells in three-dimensional coculture with stromal cells in nonwoven fabrics
    Toru Okamoto
    International Center for Biotechnology, Osaka University, Osaka, Japan
    J Artif Organs 7:194-202. 2004
    ..Heparin addition to the 3-D coculture further increased the number of progenitor cells, which may result from a synergistic effect of soluble cytokines produced by stromal cells with the sulfur group of heparin...
  24. ncbi request reprint Effect of static pressure on intracellular pH of adhesive Chinese hamster ovary cells
    Masashi Fujiwara
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita ku, Sapporo 060 8628, Japan
    J Biosci Bioeng 104:510-2. 2007
    ..1 MPa. The intracellular pH of the major population at 0.9 MPa was markedly lower than that of the single population at 0.1 MPa...
  25. ncbi request reprint Effect of pore diameter of a porous membrane on progenitor cell content during membrane-separated coculture of hematopoietic cells and a stromal cell line
    Mutsumi Takagi
    International Center for Biotechnology, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Artif Organs 6:130-7. 2003
    ....
  26. pmc Effect of the distance between adherent mesenchymal stem cell and the focus of irradiation of femtosecond laser on cell replication capacity
    Jun Sakai
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita ku, Sapporo, 060 8628, Japan
    Cytotechnology 64:323-9. 2012
    ..Finally, a novel equation of laser cell damage as a function of laser pulse energy and laser shot number is proposed...
  27. pmc Xeno-free proliferation of human bone marrow mesenchymal stem cells
    Hiroto Miwa
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita ku N13W8, Sapporo, 060 8628, Japan
    Cytotechnology 64:301-8. 2012
    ....
  28. pmc Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells
    Masashi Fujiwara
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita ku, Sapporo, 060 8628, Japan
    Cytotechnology 59:135-41. 2009
    ..25% FS media markedly decreased cell density. Consequently, the addition of heat-treated FS at low concentrations to medium may be useful for the growth of CHO cells without FCS...
  29. pmc Noninvasive estimation of cell cycle phase and proliferation rate of human mesenchymal stem cells by phase-shifting laser microscopy
    Ayako Tokumitsu
    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita ku, Sapporo, 060 8628, Japan
    Cytotechnology 59:161-7. 2009
    ..In conclusion, it is possible to estimate noninvasively the proliferation activity of MSCs population by measuring the phase shift using PLM...
  30. ncbi request reprint Hydrodynamically stable adhesion of endothelial cells onto a polypropylene hollow fiber membrane by modification with adhesive protein
    Mutsumi Takagi
    International Center for Biotechnology, Osaka University, 2 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Artif Organs 6:222-6. 2003
    ..Long-term partial cardiopulmonary bypass employing the hybrid artificial lung model should be studied further...