Masayuki Okuyama

Summary

Affiliation: Hokkaido University
Country: Japan

Publications

  1. ncbi request reprint Alpha-glucosidase mutant catalyzes "alpha-glycosynthase"-type reaction
    Masayuki Okuyama
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 66:928-33. 2002
  2. ncbi request reprint Multiple forms of alpha-glucosidase in rice seeds (Oryza sativa L., var Nipponbare)
    Hiroyuki Nakai
    Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Sapporo 060 8589, Japan
    Biochimie 89:49-62. 2007
  3. doi request reprint Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity
    Young Min Kim
    Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita ku, Sapporo 060 8589, Japan
    Appl Microbiol Biotechnol 91:329-39. 2011
  4. doi request reprint Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase
    Masayuki Okuyama
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060 8589, Japan Electronic address
    FEBS Lett 588:3213-7. 2014
  5. pmc Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand
    Atip Chantarudee
    Program of Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok10330, Thailand
    BMC Complement Altern Med 12:45. 2012
  6. pmc In vitro antiproliferative/cytotoxic activity on cancer cell lines of a cardanol and a cardol enriched from Thai Apis mellifera propolis
    Dungporn Teerasripreecha
    Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
    BMC Complement Altern Med 12:27. 2012
  7. ncbi request reprint Function and structure studies of GH family 31 and 97 α-glycosidases
    Masayuki Okuyama
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 75:2269-77. 2011
  8. ncbi request reprint Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli
    Masayuki Okuyama
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Protein Expr Purif 37:170-9. 2004
  9. ncbi request reprint Aglycone specificity of Escherichia coli alpha-xylosidase investigated by transxylosylation
    Min Sun Kang
    Division of Applied Bioscience, Hokkaido University, Sapporo, Japan
    FEBS J 274:6074-84. 2007
  10. ncbi request reprint Purification and characterization of alpha-glucosidase I from Japanese honeybee (Apis cerana japonica) and molecular cloning of its cDNA
    Jintanart Wongchawalit
    Graduate School of Agriculture, Hokkaido University, Sapporo
    Biosci Biotechnol Biochem 70:2889-98. 2006

Collaborators

  • Atsuo Kimura
  • Seiya Chiba
  • Hiroyuki Nakai
  • Hironori Hondoh
  • Min Yao
  • Kazumi Funane
  • Zui Fujimoto
  • Takeshi Yamamoto
  • Mitsuru Momma
  • Hiroshi Hara
  • Yoshiki Matsuura
  • Chanpen Chanchao
  • Yoshiyuki Tanaka
  • Nobuhiro Suzuki
  • Shukun Yu
  • Yoshiaki Kiso
  • Katsuro Yaoi
  • Nobuhisa Watanabe
  • Haruhide Mori
  • Wataru Saburi
  • Weeranuch Lang
  • Young Min Kim
  • Min Sun Kang
  • Doman Kim
  • Takayoshi Tagami
  • Momoko Kobayashi
  • Mamoru Nishimoto
  • Yuya Kumagai
  • Kyung Mo Song
  • Lukana Ngiwsara
  • Jintanart Wongchawalit
  • Juri Sadahiro
  • Aki Shinoki
  • Hee Kwon Kang
  • Jin Ha Lee
  • Momoyo Kitamura
  • Nobuo Sakairi
  • Rudee Surarit
  • Ligia O Martins
  • Jisnuson Svasti
  • Sarote Sirisansaneeyakul
  • Janjira Maneesan
  • Satoshi Ishizuka
  • Charin Thawornkuno
  • Dungporn Teerasripreecha
  • Mikihiko Kobayashi
  • Atip Chantarudee
  • Kiyoshi Kimura
  • Preecha Phuwapraisirisan
  • Hitoshi Iwaya
  • Gaku Iwai
  • Natsuko Sato
  • Kenji Fukuda
  • Saori Saito
  • Isao Tanaka
  • Fumiaki Sato
  • Masaki Kubota
  • San San Mar
  • Daichi Nakatsuka
  • Koji Kato
  • Hiroaki Rachi-Otsuka
  • Wasu Pathom-aree
  • Mariko Nishimura
  • Kazuyuki Kobayashi
  • Sónia Mendes
  • Keitaro Yamashita
  • Shinya Yamagishi
  • Eiji Yamamoto
  • Jae Sung Lee
  • Shiho Suzuki
  • Ryuichiro Suzuki
  • Tetsuya Oguma
  • Songchan Puthong
  • Shinichi Kitamura
  • Tomoe Muraki
  • Ryoko Shimizu
  • Masao Ohguchi
  • Yu Kitago
  • Fumiko Tanzawa
  • Toshitaka Nakada
  • Yu Miyaguchi
  • Yasushi Mitsuishi
  • Yukiko Takamura
  • Tsuneharu Moteki
  • Hideaki Unno
  • Siriwat Wongsiri
  • Osamu Saji
  • Hiromi Watanabe
  • Toyoyuki Ose
  • Hirokazu Matsui

Detail Information

Publications46

  1. ncbi request reprint Alpha-glucosidase mutant catalyzes "alpha-glycosynthase"-type reaction
    Masayuki Okuyama
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 66:928-33. 2002
    ..No detectable product was observed in the combination of alpha-glucosyl fluoride and PNP alpha-glucoside. This study is the first report on an "alpha-glycosynthase"-type reaction to form an alpha-glycosidic linkage...
  2. ncbi request reprint Multiple forms of alpha-glucosidase in rice seeds (Oryza sativa L., var Nipponbare)
    Hiroyuki Nakai
    Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Sapporo 060 8589, Japan
    Biochimie 89:49-62. 2007
    ..The transcripts encoding ONG2 and ONG3 were generated by alternative splicing. Members of alpha-glucosidase multigene family are differentially expressed during ripening and germinating stages in rice...
  3. doi request reprint Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity
    Young Min Kim
    Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita ku, Sapporo 060 8589, Japan
    Appl Microbiol Biotechnol 91:329-39. 2011
    ..TM-ΔCGΔ did not accept any further protease-degradation during long storage. TM-NCGΔ and TM-ΔCGΔ enhanced substrate hydrolysis, suggesting that N-VR and C-VR induce hindered substrate binding to the active site...
  4. doi request reprint Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase
    Masayuki Okuyama
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060 8589, Japan Electronic address
    FEBS Lett 588:3213-7. 2014
    ..The significant decreases in kcat/Km for hydrolysis of substrates with basic leaving groups in the absence of calcium ion confirmed that the calcium ion facilitated the leaving group departure. ..
  5. pmc Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand
    Atip Chantarudee
    Program of Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok10330, Thailand
    BMC Complement Altern Med 12:45. 2012
    ..This research aimed at a preliminary investigation of the chemical constituents and free radical scavenging activity in A. mellifera bee pollen...
  6. pmc In vitro antiproliferative/cytotoxic activity on cancer cell lines of a cardanol and a cardol enriched from Thai Apis mellifera propolis
    Dungporn Teerasripreecha
    Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
    BMC Complement Altern Med 12:27. 2012
    ..The diversity of bioactive compounds depends on the geography and climate, since these factors affect the floral diversity. Here, Apis mellifera propolis from Nan province, Thailand, was evaluated for potential anti-cancer activity...
  7. ncbi request reprint Function and structure studies of GH family 31 and 97 α-glycosidases
    Masayuki Okuyama
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 75:2269-77. 2011
    ..It appears that a catalytic nucleophile has been eliminated during the molecular evolution in the same way as a man-made nucleophile mutant enzyme, which catalyzes the inverting reaction, as in glycosynthase and chemical rescue...
  8. ncbi request reprint Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli
    Masayuki Okuyama
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Protein Expr Purif 37:170-9. 2004
    ..alpha-Glucosidase has been found for the first time in E. coli...
  9. ncbi request reprint Aglycone specificity of Escherichia coli alpha-xylosidase investigated by transxylosylation
    Min Sun Kang
    Division of Applied Bioscience, Hokkaido University, Sapporo, Japan
    FEBS J 274:6074-84. 2007
    ..Of the transxylosylation products, alpha-d-Xylp-(1-->6)-D-Manp and alpha-d-Xylp-(1-->6)-D-Fruf inhibited intestinal alpha-glucosidases...
  10. ncbi request reprint Purification and characterization of alpha-glucosidase I from Japanese honeybee (Apis cerana japonica) and molecular cloning of its cDNA
    Jintanart Wongchawalit
    Graduate School of Agriculture, Hokkaido University, Sapporo
    Biosci Biotechnol Biochem 70:2889-98. 2006
    ..Western honeybees (Apis mellifera) had three alpha-glucosidase isoenzymes (WHGase I, II, and III), in which JHGase I was considered to correspond to WHGase I...
  11. ncbi request reprint Calcium ion-dependent increase in thermostability of dextran glucosidase from Streptococcus mutans
    Momoko Kobayashi
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 75:1557-63. 2011
    ..Because similar structures and sequences are widespread in GH13, these GH13 enzymes might have been stabilized by calcium ions...
  12. pmc A novel metabolic pathway for glucose production mediated by α-glucosidase-catalyzed conversion of 1,5-anhydrofructose
    Young Min Kim
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    J Biol Chem 287:22441-4. 2012
    ..The findings indicate that α-glucosidases catalyze trans-addition. This is the first report of α-glucosidase-associated glucose formation from AF, possibly contributing to the salvage pathway of unutilized AF...
  13. pmc Structural and functional analysis of a glycoside hydrolase family 97 enzyme from Bacteroides thetaiotaomicron
    Momoyo Kitamura
    Faculty of Advanced Life Science, Graduate School of Agriculture, Hokkaido University, Sapporo 060 0810, Japan
    J Biol Chem 283:36328-37. 2008
    ..Furthermore, sequence comparison of the active site based on the structure of SusB indicated that GH97 included both retaining and inverting enzymes...
  14. doi request reprint Catalytic mechanism of retaining alpha-galactosidase belonging to glycoside hydrolase family 97
    Masayuki Okuyama
    Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita ku, Sapporo 060 8589, Japan
    J Mol Biol 392:1232-41. 2009
    ..The different positions of functional groups on the beta-face of the substrate, which seem to be due to "hopping of the functional group" during evolution, have led to divergence of catalytic mechanism within the same family...
  15. ncbi request reprint Molecular cloning of cDNA for trehalase from the European honeybee, Apis mellifera L., and its heterologous expression in Pichia pastoris
    Jin Ha Lee
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo, 060 8589, Japan
    Biosci Biotechnol Biochem 71:2256-65. 2007
    ..This is the first report of heterologous expression of insect trehalase...
  16. ncbi request reprint Function-unknown glycoside hydrolase family 31 proteins, mRNAs of which were expressed in rice ripening and germinating stages, are alpha-glucosidase and alpha-xylosidase
    Hiroyuki Nakai
    Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589 National Agricultural Research Center for Kyushu Okinawa Region, Chikugo 833 0041, Japan
    J Biochem 142:491-500. 2007
    ..The enzyme hardly hydrolysed alpha-glucosidic linkage, so that ONGX-H4 was an alpha-xylosidase. Alpha-xylosidase encoded in rice genome was found for the first time...
  17. doi request reprint Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions
    Young Min Kim
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
    FEBS J 279:3185-91. 2012
    ....
  18. doi request reprint Substrate recognition mechanism of alpha-1,6-glucosidic linkage hydrolyzing enzyme, dextran glucosidase from Streptococcus mutans
    Hironori Hondoh
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    J Mol Biol 378:913-22. 2008
    ..The comparison with the alpha-amylase suggests that Val195 of the dextran glucosidase and the corresponding residues of alpha-1,6-hydrolyzing enzymes participate in the determination of the substrate specificity of these enzymes...
  19. pmc Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase
    Wataru Saburi
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060 8589, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:774-6. 2007
    ..The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 72.72, b = 86.47, c = 104.30 A. A native data set was collected to 2.2 A resolution from a single crystal...
  20. ncbi request reprint Catalytic reaction mechanism based on alpha-secondary deuterium isotope effects in hydrolysis of trehalose by European honeybee trehalase
    Haruhide Mori
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 73:2466-73. 2009
    ....
  21. ncbi request reprint Aromatic residue on β→α loop 1 in the catalytic domain is important to the transglycosylation specificity of glycoside hydrolase family 31 α-glucosidase
    Kyung Mo Song
    Research Faculty of Agriculture, Hokkaido University
    Biosci Biotechnol Biochem 77:1759-65. 2013
    ..The mutant enzyme was found to catalyze the production of the branched oligosaccharide, 2,4-di-O-(α-D-glucopyranosyl)-D-glucopyranose, more efficiently than the wild-type enzyme. ..
  22. pmc Replacement of the catalytic nucleophile aspartyl residue of dextran glucosidase by cysteine sulfinate enhances transglycosylation activity
    Wataru Saburi
    From the Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8689, Japan
    J Biol Chem 288:31670-7. 2013
    ..This is the first study indicating that a more acidic nucleophile (-SOO(-)) enhances transglycosylation. The introduction of cysteine sulfinate as a catalytic nucleophile could be a novel approach to enhance transglycosylation. ..
  23. ncbi request reprint Amino acids in conserved region II are crucial to substrate specificity, reaction velocity, and regioselectivity in the transglucosylation of honeybee GH-13 α-glucosidases
    Lukana Ngiwsara
    Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 76:1967-74. 2012
    ..These findings indicate that the two residues are determinants of the enzymatic properties of glycoside hydrolase family 13 (GH-13) α-glucosidases and related enzymes...
  24. pmc Novel dextranase catalyzing cycloisomaltooligosaccharide formation and identification of catalytic amino acids and their functions using chemical rescue approach
    Young Min Kim
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    J Biol Chem 287:19927-35. 2012
    ..Interestingly, D189A synthesized small sized dextran from α-isomaltotetraosyl fluoride in the presence of NaN(3)...
  25. ncbi request reprint Molecular cloning of cDNAs and genes for three alpha-glucosidases from European honeybees, Apis mellifera L., and heterologous production of recombinant enzymes in Pichia pastoris
    Mamoru Nishimoto
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 71:1703-16. 2007
    ..The gene of HBGase II was not divided by intron. The gene of HBGase III was confirmed to be made up of 9 exons and 8 introns, and to be located in the region upstream the gene of HBGase I...
  26. ncbi request reprint Structural elements to convert Escherichia coli alpha-xylosidase (YicI) into alpha-glucosidase
    Masayuki Okuyama
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9 Nishi 9, Sapporo 060 8589, Japan
    FEBS Lett 580:2707-11. 2006
    ..Two mutated YicI, short loop1-enzyme and C307I/F308D, showed higher alpha-glucosidase activity than wild-type YicI. C307I/F308D, which lost alpha-xylosidase activity, was converted into alpha-glucosidase...
  27. pmc Molecular basis for the recognition of long-chain substrates by plant α-glucosidases
    Takayoshi Tagami
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    J Biol Chem 288:19296-303. 2013
    ..Subdomain b2 of SBG includes Ser-497, which was identified as the residue at subsite +4 by site-directed mutagenesis. ..
  28. ncbi request reprint Identification of essential ionizable groups and evaluation of subsite affinities in the active site of beta-D-glucosidase F1 from a Streptomyces sp
    Kenji Fukuda
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Biosci Biotechnol Biochem 66:2060-7. 2002
    ..10 kcal/mol and A2 was 4.98 kcal/mol) were considered to probably facilitate the binding of the substrate to the active site. However, the subsites 3 and 4 showed negative Ai values (A3 was -0.21 kcal/mol and A4 was -2.8 kcal/mol)...
  29. ncbi request reprint Purification, characterization, and sequence analysis of two alpha-amylase isoforms from azuki bean, Vigna angularis, showing different affinity towards beta-cyclodextrin sepharose
    San San Mar
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Biosci Biotechnol Biochem 67:1080-93. 2003
    ..The results indicated that the low affinity of VAAmy1 towards beta-cyclodextrin Sepharose was due to some modification on/near carbohydrate binding site in the limited sequence regions, resulting in higher molecular weight...
  30. doi request reprint The first alpha-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 31
    Min Sun Kang
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita ku, Sapporo 060 8589, Japan
    Biochimie 91:1434-42. 2009
    ..These hydrolysis and transglucosylation properties of LJAG31 are different from those of mold (Acremonium implicatum) alpha-1,3-glucosidase of glycoside hydrolase family 31...
  31. doi request reprint Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus TISTR1911: toward dye decolorization using a packed-bed metal affinity reactor
    Weeranuch Lang
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Bioresour Technol 150:298-306. 2013
    ..Coupling the immobilized BrAzo with glucose dehydrogenase for NADH regeneration yielded a shorter 1.5 h-degradation period that was maintained throughout 16 cycles...
  32. pmc Crystallization and preliminary X-ray analysis of alpha-xylosidase from Escherichia coli
    Momoyo Kitamura
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060 0810, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:178-9. 2005
    ..75 A. Crystals of selenomethionyl-substituted alpha-xylosidase were also obtained, which diffracted to at least 3.0 A. Based on the value of VM, the asymmetric unit in these crystals was assumed to contain six molecules...
  33. ncbi request reprint Characterization of a glycoside hydrolase family 31 α-glucosidase involved in starch utilization in Podospora anserina
    Kyung Mo Song
    Research Faculty of Agriculture, Hokkaido University
    Biosci Biotechnol Biochem 77:2117-24. 2013
    ..A site-directed mutagenesis study indicated that proteolysis had no effect on enzyme characteristics. A kinetic study indicated that the PAG possessed significant transglycosylation activity. ..
  34. ncbi request reprint Localization of alpha-glucosidases I, II, and III in organs of European honeybees, Apis mellifera L., and the origin of alpha-glucosidase in honey
    Masaki Kubota
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 68:2346-52. 2004
    ..Honey may be finally made up through the process whereby sucrose in nectar, in which glucose and fructose also are naturally contained, is hydrolyzed by secreted alpha-glucosidase III...
  35. ncbi request reprint Enzymatic synthesis of Acarviosyl-maltooligosaccharides using disproportionating enzyme 1
    Takayoshi Tagami
    Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 77:312-9. 2013
    ..ACn can be useful tools as new inhibitors of glycoside hydrolases...
  36. ncbi request reprint Glucoamylase originating from Schwanniomyces occidentalis is a typical alpha-glucosidase
    Fumiaki Sato
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
    Biosci Biotechnol Biochem 69:1905-13. 2005
    ..These were no similarities to the so-called glucoamylases. It was concluded that the enzyme and also S. occidentalis glucoamylase, had been already reported, were typical alpha-glucosidases, and not glucoamylase...
  37. doi request reprint Different molecular complexity of linear-isomaltomegalosaccharides and β-cyclodextrin on enhancing solubility of azo dye ethyl red: towards dye biodegradation
    Weeranuch Lang
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Bioresour Technol 169:518-24. 2014
    ..The dye intermission in series exhibited successful removal with at least 5 cycles was economically feasible...
  38. doi request reprint Structural insights into the catalytic reaction that is involved in the reorientation of Trp238 at the substrate-binding site in GH13 dextran glucosidase
    Momoko Kobayashi
    Graduate School of Life Science, Hokkaido University, Kita 10, Nishi 8, Kita ku, Sapporo 060 0810, Japan
    FEBS Lett 589:484-9. 2015
    ..Different conformations of Trp238 in each reaction stage indicated its flexibility. Considering the results of kinetic analyses, such flexibility may reflect a requirement for the reaction mechanism of SmDG...
  39. doi request reprint Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench
    Weeranuch Lang
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    J Environ Manage 132:155-64. 2014
    ..0% NaCl in reaction media. Therefore, this bacterial system has potential for dye decolorization bioprocesses occurring at high temperature and salt concentrations e.g. for cleaning dye-containing saline wastewaters. ..
  40. pmc The delay in the development of experimental colitis from isomaltosyloligosaccharides in rats is dependent on the degree of polymerization
    Hitoshi Iwaya
    Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
    PLoS ONE 7:e50658. 2012
    ..There is wide variation in the degree of polymerization (DP) in Dex and IMO (short-sized IMO, S-IMO; long-sized IMO, L-IMO), and the physiological influence of these compounds may be dependent on their DP...
  41. doi request reprint A novel mechanism for the promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine
    Aki Shinoki
    Hokkaido University, Kita 9 Nishi 9, Kita ku, Sapporo 060 8589, Japan
    Food Chem 136:293-6. 2013
    ..These results suggest that the ingestion of α-1,6-glucosaccharides promotes Q3G bioavailability...
  42. ncbi request reprint Structural elements in dextran glucosidase responsible for high specificity to long chain substrate
    Wataru Saburi
    Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Biochim Biophys Acta 1764:688-98. 2006
    ..subtilis oligo-1,6-glucosidase showed lower preference for long-chain substrates, indicating that both Trp238 and short beta-->alpha loop 4 were important for high activity to long-chain substrates...
  43. doi request reprint Biochemical properties and substrate recognition mechanism of GH31 α-glucosidase from Bacillus sp. AHU 2001 with broad substrate specificity
    Wataru Saburi
    Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita ku, Sapporo 060 8589, Japan
    Biochimie 108:140-8. 2015
    ..V543A showed 1.8-3.5-fold higher hydrolytic activities toward all substrates other than neotrehalose compared with wild type, although its preference for isomaltose was unchanged. ..
  44. doi request reprint Extracellular and cell-associated forms of Gluconobacter oxydans dextran dextrinase change their localization depending on the cell growth
    Juri Sadahiro
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Biochem Biophys Res Commun 456:500-5. 2015
    ..During the early growth stage, DDase was exclusively liberated into the medium (DDext), and the cell-associated form (DDint) appeared after depletion of glucose from the medium...
  45. doi request reprint Structural elements responsible for the glucosidic linkage-selectivity of a glycoside hydrolase family 13 exo-glucosidase
    Wataru Saburi
    Research Faculty of Agriculture, Hokkaido University, Sapporo 060 8689, Japan
    FEBS Lett 589:865-9. 2015
    ..Consequently, the three residues are structural elements for recognition of the α-(1→6)-glucosidic linkage. ..
  46. ncbi request reprint Purification and characterization of Acremonium implicatum alpha-glucosidase having regioselectivity for alpha-1,3-glucosidic linkage
    Takeshi Yamamoto
    Nihon Shokuhin Kako Co Ltd, 30 Tajima Fuji Shizuoka 417 8530, Japan
    Biochim Biophys Acta 1700:189-98. 2004
    ..Eight kinds of transglucosylation products were synthesized from maltose, in which 3(2)-O-alpha-nigerosyl-maltose and 3(2)-O-alpha-maltosyl-maltose were novel saccharides...