H Okuyama

Summary

Affiliation: Hokkaido University
Country: Japan

Publications

  1. doi request reprint Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation.
    Akio Ueno
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo, 060 0810, Japan
    World J Microbiol Biotechnol 23:1739-45. 2007
  2. pmc Hydrophilic and Hydrophobic Compounds Antithetically Affect the Growth of Eicosapentaenoic Acid-Synthesizing Escherichia coli Recombinants
    Ryuji Hori
    Course in Environmental Molecular Biology and Microbial Ecology, Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    Open Microbiol J 5:114-8. 2011
  3. pmc The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids
    Shinji Sugihara
    Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University, Kita ku, Sapporo, Japan
    FEMS Microbiol Lett 307:207-11. 2010
  4. ncbi request reprint Glycosylphosphatidylinositol-anchored proteins found in the moss Marchantia polymorpha
    H Okuyama
    Graduate School Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochem Soc Trans 28:589-90. 2000
  5. pmc Significance of antioxidative functions of eicosapentaenoic and docosahexaenoic acids in marine microorganisms
    Hidetoshi Okuyama
    Faculty of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    Appl Environ Microbiol 74:570-4. 2008
  6. ncbi request reprint In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B
    Hidetoshi Okuyama
    Faculty of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo, 060 0810, Japan
    Biotechnol Lett 29:1977-81. 2007
  7. pmc Bacterial genes responsible for the biosynthesis of eicosapentaenoic and docosahexaenoic acids and their heterologous expression
    Hidetoshi Okuyama
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Appl Environ Microbiol 73:665-70. 2007
  8. pmc The glycosylphosphatidylinositol-anchored phosphatase from Spirodela oligorrhiza is a purple acid phosphatase
    H Nakazato
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    Plant Physiol 118:1015-20. 1998
  9. ncbi request reprint Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium
    I Yumoto
    Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, Sapporo, Japan
    Int J Syst Bacteriol 48:1357-62. 1998
  10. ncbi request reprint Characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. strain SCRC-2738
    Y Orikasa
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Cell Mol Biol (Noisy-le-grand) 50:625-30. 2004

Collaborators

  • I Yumoto
  • T Okamoto
  • N Morita
  • M Tanaka
  • K Washio
  • K Watanabe
  • Y Ito
  • H Matsuyama
  • K Kato
  • H Iwata
  • R Yu
  • Hidenori Hayashi
  • Yoshitake Orikasa
  • Takanori Nishida
  • Shinji Sugihara
  • Akio Ueno
  • Ryuji Hori
  • Akiko Yamada
  • Akira Hase
  • Zakia Perveen
  • Kikue Hirota
  • Yoshinobu Nodasaka
  • Mohammad Hasanuzzaman
  • Seiji Yamauchi
  • Y Orikasa
  • Takuji Ohwada
  • Yutaka Yano
  • Yohko Yamada
  • Yusuke Yamamoto
  • Tomoko Takagi
  • Takako Kaneko
  • H Nakazato
  • Kazuhiro Kogame
  • Hitomi Ando
  • A Yamada
  • Szilvia M Zsiros
  • Kathryn M Umadhay-Briones
  • Yoshitaka Nishiyama
  • T Nishida
  • K Haraguchi
  • G A Thompson
  • M Nishikoori

Detail Information

Publications24

  1. doi request reprint Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation.
    Akio Ueno
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo, 060 0810, Japan
    World J Microbiol Biotechnol 23:1739-45. 2007
    ..The bioaugmentation capacity of isolate J in soil microcosms contaminated with diesel oil was much higher than that of P. aeruginosa strain WatG. The possibility of using isolate J for autochthonous bioaugmentation is discussed...
  2. pmc Hydrophilic and Hydrophobic Compounds Antithetically Affect the Growth of Eicosapentaenoic Acid-Synthesizing Escherichia coli Recombinants
    Ryuji Hori
    Course in Environmental Molecular Biology and Microbial Ecology, Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    Open Microbiol J 5:114-8. 2011
    ..The cell surface hydrophobicity of DH5αEPA+ was higher than that of DH5αEPA-, suggesting that EPA may operate as a structural constituent in the cell membrane to affect the entry and efflux of hydrophilic and hydrophobic inhibitors...
  3. pmc The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids
    Shinji Sugihara
    Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University, Kita ku, Sapporo, Japan
    FEMS Microbiol Lett 307:207-11. 2010
    ..Here, we showed that all Sfp-type PPTases may have the potential to promote the biosynthesis of long-chain n-3 polyunsaturated fatty acids...
  4. ncbi request reprint Glycosylphosphatidylinositol-anchored proteins found in the moss Marchantia polymorpha
    H Okuyama
    Graduate School Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Biochem Soc Trans 28:589-90. 2000
    ..Thus the secretory 47-kDa protein of M. polymorpha was concluded to be a GPI-anchored protein...
  5. pmc Significance of antioxidative functions of eicosapentaenoic and docosahexaenoic acids in marine microorganisms
    Hidetoshi Okuyama
    Faculty of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    Appl Environ Microbiol 74:570-4. 2008
  6. ncbi request reprint In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B
    Hidetoshi Okuyama
    Faculty of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo, 060 0810, Japan
    Biotechnol Lett 29:1977-81. 2007
    ..At the same time, the amount of phospholipid (5 mg) per whole culture also increased 3-fold. Hence, phospholipid/total lipid increased from 13% to 67%. High levels of DHA (more than 50% of total) were maintained in phosphatidylcholine...
  7. pmc Bacterial genes responsible for the biosynthesis of eicosapentaenoic and docosahexaenoic acids and their heterologous expression
    Hidetoshi Okuyama
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Appl Environ Microbiol 73:665-70. 2007
  8. pmc The glycosylphosphatidylinositol-anchored phosphatase from Spirodela oligorrhiza is a purple acid phosphatase
    H Nakazato
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    Plant Physiol 118:1015-20. 1998
    ..These results, together with previous findings, strongly indicate that the GPI-anchored phosphatase of S. oligorrhiza is a PAP...
  9. ncbi request reprint Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium
    I Yumoto
    Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, Sapporo, Japan
    Int J Syst Bacteriol 48:1357-62. 1998
    ..Accordingly, the name Colwellia maris is proposed. The type strain is ABE-1T (= JCM 10085T)...
  10. ncbi request reprint Characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. strain SCRC-2738
    Y Orikasa
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    Cell Mol Biol (Noisy-le-grand) 50:625-30. 2004
    ..There was a strict upper temperature limit for EPA biosynthesis: no EPA was synthesized at 30 degrees C in E. coli transformants carrying any gene construct for EPA biosynthesis...
  11. ncbi request reprint Characterization of the gene encoding the beta-lactamase of the psychrophilic marine bacterium Moritella marina strain MP-1
    M Tanaka
    Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, AIST/MITI, Sapporo, Japan
    Biosci Biotechnol Biochem 65:666-9. 2001
    ..E. coli transformed with the plasmid containing mbla grew on an ampicillin-containing plate at 37 degrees C but not at 42 degrees C, suggesting that the beta-lactamase of this bacterium is heat-labile...
  12. ncbi request reprint Shewanella pneumatophori sp. nov., an eicosapentaenoic acid-producing marine bacterium isolated from the intestines of Pacific mackerel (Pneumatophorus japonicus)
    Kikue Hirota
    Research Institute of Genome Based Biofactory, National Institute of Advanced Industrial Science and Technology, Tsukisamu Higashi, Toyohira ku, Sapporo 062 8517, Japan
    Int J Syst Evol Microbiol 55:2355-9. 2005
    ..nov. is proposed. The type strain is SCRC-2738T (=JCM 13187T=NCIMB 14060T)...
  13. ncbi request reprint Escherichia coli engineered to produce eicosapentaenoic acid becomes resistant against oxidative damages
    Takanori Nishida
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    FEBS Lett 580:2731-5. 2006
    ..We discuss the possible role of EPA in partially blocking the penetration of H(2)O(2) into cells through membranes containing EPA...
  14. ncbi request reprint A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1
    Yoshitake Orikasa
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060 0810, Japan
    FEBS Lett 580:4423-9. 2006
    ..This suggests that pfaE encodes a PPTase required for producing n-3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1...
  15. ncbi request reprint Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli
    Yoshitake Orikasa
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, 060 0810, Japan
    Biotechnol Lett 28:1841-7. 2006
    ..The maximum production of DHA (5% of total fatty acids) was observed at 15 degrees C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode...
  16. ncbi request reprint Cultivation of microorganisms in the cultural medium made from squid internal organs and accumulation of polyunsaturated fatty acids in the cells
    Naoki Morita
    Research Institute of Genome Based Biofactory, National Institute of Advanced Industrial Science and Technology, 062 8517 Toyohira ku, Sapporo, Japan
    Biotechnol Lett 27:933-41. 2005
    ....
  17. ncbi request reprint Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that co-express eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA
    Yoshitake Orikasa
    Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo, 060 0810, Japan
    Biotechnol Lett 29:803-9. 2007
    ..This suggests that the retention of a large DNA insert in the vector and the accumulation of the resulting protein, but not the catalytic activity of VktA catalase, would potentially be able to increase the content of EPA...
  18. ncbi request reprint Enhancement of polyunsaturated fatty acid production by cerulenin treatment in polyunsaturated fatty acid-producing bacteria
    Naoki Morita
    Research Institute of Genome Based Biofactory, National Institute of Advanced Industrial Science and Technology AIST, Toyohira ku, Sapporo, 062 8517, Japan
    Biotechnol Lett 27:389-93. 2005
    ..6 to 8 mg l-1. The use of cerulenin is, therefore, advantageous to increase the content of intracellular polyunsaturated fatty acids (PUFA) in particular PUFA-containing phospholipids in bacterial cells...
  19. ncbi request reprint The antioxidative function of eicosapentaenoic acid in a marine bacterium, Shewanella marinintestina IK-1
    Takanori Nishida
    Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    FEBS Lett 581:4212-6. 2007
    ..These results suggest that cellular EPA can directly protect cells against oxidative damage by shielding the entry of exogenously added H(2)O(2) in S. marinintestina IK-1...
  20. ncbi request reprint An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid
    Shinji Sugihara
    Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, Japan
    Biotechnol Lett 30:411-4. 2008
    ..This is the first report showing that the EntD-like PPTase is involved in producing n-3 polyunsaturated fatty acids...
  21. ncbi request reprint Isolation and characterization of a novel thraustochytrid-like microorganism that efficiently produces docosahexaenoic acid
    Zakia Perveen
    Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Kita ku, 060 0810, Japan
    Biotechnol Lett 28:197-202. 2006
    ..It had a growth rate of 0.38 h(-1). The DHA production rate of 2.8 +/- 0.7 g l(-1) day(-1) is the highest value reported for any microorganism...
  22. ncbi request reprint The cell membrane-shielding function of eicosapentaenoic acid for Escherichia coli against exogenously added hydrogen peroxide
    Takanori Nishida
    Graduate School of Environmental Earth Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    FEBS Lett 580:6690-4. 2006
    ..These results suggest that cellular EPA can directly protect cells against oxidative damage by shielding the entry of exogenously added H(2)O(2)...
  23. ncbi request reprint Isolation, identification, and characterization of a novel, oil-degrading bacterium, Pseudomonas aeruginosa T1
    Mohammad Hasanuzzaman
    Graduate School of Environmental Earth Sciencee, Hokkaido University, Sapporo 064 0804, Japan
    Curr Microbiol 49:108-14. 2004
    ..The 16S rRNA gene sequence analysis and DNA-DNA hybridization revealed it as a new strain of Pseudomonas aeruginosa. The type strain was T1...
  24. ncbi request reprint Gene structure and transcriptional regulation of dnaK and dnaJ genes from a psychrophilic bacterium, Colwellia maris
    Seiji Yamauchi
    Graduate School of Science and Engineering, Ehime University, Bunkyo cho, Matsuyama 790 8577, Japan
    Extremophiles 8:283-90. 2004
    ..In addition, the level of the induction of the dnaJ gene was much lower than that of the dnaK gene. These findings together revealed several specific features of the heat shock response at a relatively low temperature in psychrophiles...