Hirokazu Kouguchi

Summary

Affiliation: Hokkaido Institute of Public Health
Country: Japan

Publications

  1. ncbi request reprint Characterization of various recombinant antigens from Echinococcus multilocularis for use in the immunodiagnosis
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, Kita ku, Sapporo 060 0819, Japan
    Protein J 24:57-64. 2005
  2. doi request reprint Echinococcus multilocularis: two-dimensional Western blotting method for the identification and expression analysis of immunogenic proteins in infected dogs
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19 W12, Kita ku, Sapporo 060 0819, Japan
    Exp Parasitol 124:238-43. 2010
  3. ncbi request reprint Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis
    Yoshinobu Katoh
    Department of Biological Science, Center for Infectious Disease Prevention, Hokkaido Institute of Public Health, Sapporo, Hokkaido 060 0819, Japan
    Biochim Biophys Acta 1780:1-6. 2008
  4. doi request reprint Echinococcus multilocularis: purification and characterization of glycoprotein antigens with serodiagnostic potential for canine infection
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19 W12, Kita ku, Sapporo, Japan
    Exp Parasitol 128:50-6. 2011
  5. doi request reprint Molecular cloning and characterization of major vault protein of Echinococcus multilocularis
    Akiko Goto
    Hokkaido Institute of Public Health, Sapporo 060 0819, Japan
    Exp Parasitol 134:102-8. 2013
  6. pmc Characterization of a surface glycoprotein from Echinococcus multilocularis and its mucosal vaccine potential in dogs
    Hirokazu Kouguchi
    Department of Infectious Diseases, Hokkaido Institute of Public Health, Sapporo, Japan
    PLoS ONE 8:e69821. 2013
  7. doi request reprint Identification of genetic loci affecting the establishment and development of Echinococcus multilocularis larvae in mice
    Ryo Nakao
    Department of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita 20, Nishi 10, Kita ku, Sapporo, Hokkaido 001 0020, Japan
    Int J Parasitol 41:1121-8. 2011
  8. ncbi request reprint The vaccination potential of EMY162 antigen against Echinococcus multilocularis infection
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19, W12, Kita ku, Sapporo 060 0819, Japan
    Biochem Biophys Res Commun 363:915-20. 2007
  9. ncbi request reprint Early and presymptomatic detection of Wilson's disease at the mandatory 3-year-old medical health care examination in Hokkaido Prefecture with the use of a novel automated urinary ceruloplasmin assay
    Kenji Nakayama
    Hokkaido Institute of Public Health, North 19 West 12, Kita ku, Sapporo 060 0819, Hokkaido, Japan
    Mol Genet Metab 94:363-7. 2008
  10. ncbi request reprint First detection of Echinococcus multilocularis infection in two species of nonhuman primates raised in a zoo: a fatal case in Cercopithecus diana and a strongly suspected case of spontaneous recovery in Macaca nigra
    Kimiaki Yamano
    Department of Infectious Diseases, Hokkaido Institute of Public Health, North 19, West 12, Kitaku, Sapporo 060 0819, Hokkaido, Japan Electronic address
    Parasitol Int 63:621-6. 2014

Collaborators

  • Jun Matsumoto
  • Akiko Goto
  • Nobuya Sasaki
  • Chihiro Sugimoto
  • Kunihiko Kobayashi
  • Norikazu Shimizu
  • Tadashi Ariga
  • Akiko Saito
  • K Yamano
  • Yoshimasa Sagane
  • Tomonori Suzuki
  • Kimiko Hasegawa
  • Shingo Mutoh
  • Yoshinobu Katoh
  • Toshihiro Watanabe
  • Tohru Ohyama
  • Ryo Nakao
  • Keiji Oguma
  • Kinpei Yagi
  • Yuzaburo Oku
  • Tomohiro Suzuki
  • Kenji Nakayama
  • Yukako Fujinaga
  • Daisuke Torigoe
  • Zhisheng Dang
  • Takashi Agui
  • Ayo Yila Simon
  • Yayoi Kameda
  • Setsuo Kanzaki
  • Mitsuru Kubota
  • Hiroshi Honma
  • Akihiko Ohtake
  • Hitoshi Itami
  • Masahide Tamura
  • Tsugutoshi Aoki
  • Kenji Fujieda
  • Yukiharu Sawada
  • Naoshi Ichihara
  • Eiji Katsura
  • Kazuhiko Nishimura
  • Kouichi Takeshi
  • Hiroaki Sato
  • Akihito Yamano
  • Arihide Kamaguchi
  • Hiroyuki Sunagawa
  • Shigehiro Obata

Detail Information

Publications16

  1. ncbi request reprint Characterization of various recombinant antigens from Echinococcus multilocularis for use in the immunodiagnosis
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, Kita ku, Sapporo 060 0819, Japan
    Protein J 24:57-64. 2005
    ..In addition, the specific antibodies against #7 and #8s reacted with specific antigens in crude extract of E. multilocularis cyst, indicating that these antigens retained antigenicity common to native EmII/3 and AFFP, respectively...
  2. doi request reprint Echinococcus multilocularis: two-dimensional Western blotting method for the identification and expression analysis of immunogenic proteins in infected dogs
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19 W12, Kita ku, Sapporo 060 0819, Japan
    Exp Parasitol 124:238-43. 2010
    ..Furthermore, recombinant hsp20 showed specific reactivity to the sera from infected dogs, suggesting that this molecule may facilitate the development of a practical vaccine...
  3. ncbi request reprint Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis
    Yoshinobu Katoh
    Department of Biological Science, Center for Infectious Disease Prevention, Hokkaido Institute of Public Health, Sapporo, Hokkaido 060 0819, Japan
    Biochim Biophys Acta 1780:1-6. 2008
    ..Immune responses to the recombinant EMY162 were studied by using serum from dogs infected with E. multilocularis. Strong IgG immune responses were detected in Western blots...
  4. doi request reprint Echinococcus multilocularis: purification and characterization of glycoprotein antigens with serodiagnostic potential for canine infection
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19 W12, Kita ku, Sapporo, Japan
    Exp Parasitol 128:50-6. 2011
    ....
  5. doi request reprint Molecular cloning and characterization of major vault protein of Echinococcus multilocularis
    Akiko Goto
    Hokkaido Institute of Public Health, Sapporo 060 0819, Japan
    Exp Parasitol 134:102-8. 2013
    ....
  6. pmc Characterization of a surface glycoprotein from Echinococcus multilocularis and its mucosal vaccine potential in dogs
    Hirokazu Kouguchi
    Department of Infectious Diseases, Hokkaido Institute of Public Health, Sapporo, Japan
    PLoS ONE 8:e69821. 2013
    ..multilocularis infection in dogs. Thus, our data indicated that mucosal immunization using surface antigens will be an important tool to facilitate the development of practical vaccines for definitive hosts...
  7. doi request reprint Identification of genetic loci affecting the establishment and development of Echinococcus multilocularis larvae in mice
    Ryo Nakao
    Department of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita 20, Nishi 10, Kita ku, Sapporo, Hokkaido 001 0020, Japan
    Int J Parasitol 41:1121-8. 2011
    ....
  8. ncbi request reprint The vaccination potential of EMY162 antigen against Echinococcus multilocularis infection
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19, W12, Kita ku, Sapporo 060 0819, Japan
    Biochem Biophys Res Commun 363:915-20. 2007
    ..These results may help in the development of a practical vaccine to reduce the level of alveolar echinococcosis in humans...
  9. ncbi request reprint Early and presymptomatic detection of Wilson's disease at the mandatory 3-year-old medical health care examination in Hokkaido Prefecture with the use of a novel automated urinary ceruloplasmin assay
    Kenji Nakayama
    Hokkaido Institute of Public Health, North 19 West 12, Kita ku, Sapporo 060 0819, Hokkaido, Japan
    Mol Genet Metab 94:363-7. 2008
    ....
  10. ncbi request reprint First detection of Echinococcus multilocularis infection in two species of nonhuman primates raised in a zoo: a fatal case in Cercopithecus diana and a strongly suspected case of spontaneous recovery in Macaca nigra
    Kimiaki Yamano
    Department of Infectious Diseases, Hokkaido Institute of Public Health, North 19, West 12, Kitaku, Sapporo 060 0819, Hokkaido, Japan Electronic address
    Parasitol Int 63:621-6. 2014
    ..These findings strongly suggest that the animal had recovered spontaneously. Until this study, spontaneous recovery from E. multilocularis infection in a nonhuman primate had never been reported. ..
  11. ncbi request reprint Quantitative detection of gene expression and toxin complex produced by Clostridium botulinum serotype D strain 4947
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19, W12, Kita ku, Sapporo 060 0819, Japan
    J Microbiol Methods 67:416-23. 2006
    ..Western blot analysis demonstrated that TC species in cell lysate were initially observed in the mid-exponential phase, while extracellular TCs were detected subsequently in the early stationary phase...
  12. ncbi request reprint Characterization of the interaction between subunits of the botulinum toxin complex produced by serotype D through tryptic susceptibility of the isolated components and complex forms
    Tomonori Suzuki
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099 2493, Japan
    Microbiology 151:1475-83. 2005
    ....
  13. ncbi request reprint Characterization of toxin complex produced by a unique strain of Clostridium botulinum serotype D 4947
    Kimiko Hasegawa
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099 2493, Japan
    Protein J 23:371-8. 2004
    ..We have established a new method to separate the unique D-4947 NT from the complex, which will yield valuable information on structure of botulinum toxin...
  14. ncbi request reprint Complete subunit structure of the Clostridium botulinum type D toxin complex via intermediate assembly with nontoxic components
    Shingo Mutoh
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099 2493, Japan
    Biochemistry 42:10991-7. 2003
    ..In conclusion, the complete subunit composition of mature L-TC is deduced to be a dodecamer assembled by a single NT, a single NTNHA, two HA-70, four HA-33, and four HA-17 molecules...
  15. ncbi request reprint Spontaneous nicking in the nontoxic-nonhemagglutinin component of the Clostridium botulinum toxin complex
    Yoshimasa Sagane
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099 2493, Japan
    Biochem Biophys Res Commun 292:434-40. 2002
    ....
  16. ncbi request reprint In vitro reconstitution of the Clostridium botulinum type D progenitor toxin
    Hirokazu Kouguchi
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099 2493, Japan
    J Biol Chem 277:2650-6. 2002
    ..We conclude that the M toxin forms first by assembly of NT with NTNHA and is subsequently converted to the L toxin by assembly with HA-70 and HA-33/17...