Hirokazu Kouguchi

Summary

Affiliation: Hokkaido Institute of Public Health
Country: Japan

Publications

  1. ncbi Characterization of various recombinant antigens from Echinococcus multilocularis for use in the immunodiagnosis
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, Kita ku, Sapporo 060 0819, Japan
    Protein J 24:57-64. 2005
  2. ncbi Echinococcus multilocularis: two-dimensional Western blotting method for the identification and expression analysis of immunogenic proteins in infected dogs
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19 W12, Kita ku, Sapporo 060 0819, Japan
    Exp Parasitol 124:238-43. 2010
  3. ncbi Echinococcus multilocularis: purification and characterization of glycoprotein antigens with serodiagnostic potential for canine infection
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19 W12, Kita ku, Sapporo, Japan
    Exp Parasitol 128:50-6. 2011
  4. ncbi Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis
    Yoshinobu Katoh
    Department of Biological Science, Center for Infectious Disease Prevention, Hokkaido Institute of Public Health, Sapporo, Hokkaido 060 0819, Japan
    Biochim Biophys Acta 1780:1-6. 2008
  5. ncbi Identification of genetic loci affecting the establishment and development of Echinococcus multilocularis larvae in mice
    Ryo Nakao
    Department of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita 20, Nishi 10, Kita ku, Sapporo, Hokkaido 001 0020, Japan
    Int J Parasitol 41:1121-8. 2011
  6. ncbi Early and presymptomatic detection of Wilson's disease at the mandatory 3-year-old medical health care examination in Hokkaido Prefecture with the use of a novel automated urinary ceruloplasmin assay
    Kenji Nakayama
    Hokkaido Institute of Public Health, North 19 West 12, Kita ku, Sapporo 060 0819, Hokkaido, Japan
    Mol Genet Metab 94:363-7. 2008
  7. ncbi The vaccination potential of EMY162 antigen against Echinococcus multilocularis infection
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19, W12, Kita ku, Sapporo 060 0819, Japan
    Biochem Biophys Res Commun 363:915-20. 2007
  8. ncbi Quantitative detection of gene expression and toxin complex produced by Clostridium botulinum serotype D strain 4947
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19, W12, Kita-ku, Sapporo 060-0819, Japan
    J Microbiol Methods 67:416-23. 2006
  9. ncbi Characterization of the interaction between subunits of the botulinum toxin complex produced by serotype D through tryptic susceptibility of the isolated components and complex forms
    Tomonori Suzuki
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan
    Microbiology 151:1475-83. 2005
  10. ncbi Characterization of toxin complex produced by a unique strain of Clostridium botulinum serotype D 4947
    Kimiko Hasegawa
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493, Japan
    Protein J 23:371-8. 2004

Collaborators

  • Jun Matsumoto
  • Akiko Saito
  • Tadashi Ariga
  • Norikazu Shimizu
  • Kunihiko Kobayashi
  • K Yamano
  • Yoshimasa Sagane
  • Tomonori Suzuki
  • Kimiko Hasegawa
  • Shingo Mutoh
  • Tohru Ohyama
  • Yoshinobu Katoh
  • Toshihiro Watanabe
  • Ryo Nakao
  • Keiji Oguma
  • Yuzaburo Oku
  • Kinpei Yagi
  • Kenji Nakayama
  • Tomohiro Suzuki
  • Yukako Fujinaga
  • Chihiro Sugimoto
  • Yayoi Kameda
  • Daisuke Torigoe
  • Nobuya Sasaki
  • Takashi Agui
  • Zhisheng Dang
  • Ayo Yila Simon
  • Kenji Fujieda
  • Yukiharu Sawada
  • Setsuo Kanzaki
  • Akihiko Ohtake
  • Hitoshi Itami
  • Mitsuru Kubota
  • Masahide Tamura
  • Naoshi Ichihara
  • Tsugutoshi Aoki
  • Akiko Goto
  • Hiroshi Honma
  • Kazuhiko Nishimura
  • Eiji Katsura
  • Kouichi Takeshi
  • Arihide Kamaguchi
  • Akihito Yamano
  • Hiroaki Sato
  • Hiroyuki Sunagawa
  • Shigehiro Obata

Detail Information

Publications13

  1. ncbi Characterization of various recombinant antigens from Echinococcus multilocularis for use in the immunodiagnosis
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, Kita ku, Sapporo 060 0819, Japan
    Protein J 24:57-64. 2005
    ..In addition, the specific antibodies against #7 and #8s reacted with specific antigens in crude extract of E. multilocularis cyst, indicating that these antigens retained antigenicity common to native EmII/3 and AFFP, respectively...
  2. ncbi Echinococcus multilocularis: two-dimensional Western blotting method for the identification and expression analysis of immunogenic proteins in infected dogs
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19 W12, Kita ku, Sapporo 060 0819, Japan
    Exp Parasitol 124:238-43. 2010
    ..Furthermore, recombinant hsp20 showed specific reactivity to the sera from infected dogs, suggesting that this molecule may facilitate the development of a practical vaccine...
  3. ncbi Echinococcus multilocularis: purification and characterization of glycoprotein antigens with serodiagnostic potential for canine infection
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19 W12, Kita ku, Sapporo, Japan
    Exp Parasitol 128:50-6. 2011
    ....
  4. ncbi Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis
    Yoshinobu Katoh
    Department of Biological Science, Center for Infectious Disease Prevention, Hokkaido Institute of Public Health, Sapporo, Hokkaido 060 0819, Japan
    Biochim Biophys Acta 1780:1-6. 2008
    ..Immune responses to the recombinant EMY162 were studied by using serum from dogs infected with E. multilocularis. Strong IgG immune responses were detected in Western blots...
  5. ncbi Identification of genetic loci affecting the establishment and development of Echinococcus multilocularis larvae in mice
    Ryo Nakao
    Department of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita 20, Nishi 10, Kita ku, Sapporo, Hokkaido 001 0020, Japan
    Int J Parasitol 41:1121-8. 2011
    ....
  6. ncbi Early and presymptomatic detection of Wilson's disease at the mandatory 3-year-old medical health care examination in Hokkaido Prefecture with the use of a novel automated urinary ceruloplasmin assay
    Kenji Nakayama
    Hokkaido Institute of Public Health, North 19 West 12, Kita ku, Sapporo 060 0819, Hokkaido, Japan
    Mol Genet Metab 94:363-7. 2008
    ....
  7. ncbi The vaccination potential of EMY162 antigen against Echinococcus multilocularis infection
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19, W12, Kita ku, Sapporo 060 0819, Japan
    Biochem Biophys Res Commun 363:915-20. 2007
    ..These results may help in the development of a practical vaccine to reduce the level of alveolar echinococcosis in humans...
  8. ncbi Quantitative detection of gene expression and toxin complex produced by Clostridium botulinum serotype D strain 4947
    Hirokazu Kouguchi
    Hokkaido Institute of Public Health, N19, W12, Kita-ku, Sapporo 060-0819, Japan
    J Microbiol Methods 67:416-23. 2006
    ..Western blot analysis demonstrated that TC species in cell lysate were initially observed in the mid-exponential phase, while extracellular TCs were detected subsequently in the early stationary phase...
  9. ncbi Characterization of the interaction between subunits of the botulinum toxin complex produced by serotype D through tryptic susceptibility of the isolated components and complex forms
    Tomonori Suzuki
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan
    Microbiology 151:1475-83. 2005
    ....
  10. ncbi Characterization of toxin complex produced by a unique strain of Clostridium botulinum serotype D 4947
    Kimiko Hasegawa
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493, Japan
    Protein J 23:371-8. 2004
    ..We have established a new method to separate the unique D-4947 NT from the complex, which will yield valuable information on structure of botulinum toxin...
  11. ncbi Complete subunit structure of the Clostridium botulinum type D toxin complex via intermediate assembly with nontoxic components
    Shingo Mutoh
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan
    Biochemistry 42:10991-7. 2003
    ..In conclusion, the complete subunit composition of mature L-TC is deduced to be a dodecamer assembled by a single NT, a single NTNHA, two HA-70, four HA-33, and four HA-17 molecules...
  12. ncbi Spontaneous nicking in the nontoxic-nonhemagglutinin component of the Clostridium botulinum toxin complex
    Yoshimasa Sagane
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan
    Biochem Biophys Res Commun 292:434-40. 2002
    ....
  13. ncbi In vitro reconstitution of the Clostridium botulinum type D progenitor toxin
    Hirokazu Kouguchi
    Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan
    J Biol Chem 277:2650-6. 2002
    ..We conclude that the M toxin forms first by assembly of NT with NTNHA and is subsequently converted to the L toxin by assembly with HA-70 and HA-33/17...